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Biocontrol Science and Technology Publication details, including instructions for authors and subscription information: http://www.informaworld.com/smpp/title~content=t713409232 No-choice host range tests for Allotropa sp. near mecrida, a parasitoid of the pink hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae) William J. Roltsch a; Lawrence R. Ertle b; Dale E. Meyerdirk c a California Department of Food & Agriculture, Biological Control Program, Sacramento, CA, USA b US Department of Agriculture, Agricultural Research Service, Beneficial Insects Research Laboratory, Newark, DE, USA c US Department of Agriculture, Animal and Plant Health Inspection Service, Plant Protection and Quarantine, Pest Detection and Management Programs, MD, USA

Online Publication Date: 01 January 2007 To cite this Article: Roltsch, William J., Ertle, Lawrence R. and Meyerdirk, Dale E. (2007) 'No-choice host range tests for Allotropa sp. near mecrida, a parasitoid of the pink hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae)', Biocontrol Science and Technology, 17:9, 977 - 981 To link to this article: DOI: 10.1080/09583150701661661 URL: http://dx.doi.org/10.1080/09583150701661661

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TX, Agricultural conducted Agriculture, Edinburg, Plant were in (USDA-APHIS), Center evaluations Service Protection The Plant Inspection Mission Health Quarantine, Plant and Protection and Animal commonly Agriculture, of a represents Valley. mealybug Imperial solenopsis in The species that California. native mealybug, papaya found in the species of exception resident the with are genera, several represent they because mealybug, solenopsis and 978 diin eea rs oaosruswr de ossanmayusoe the 20 over limited, replicate. mealybugs was each in material sustain used host were to parasitoids when male instances added five and some were female In Five sprouts trial. the potato of duration fresh several addition, by attacked mealybug long-tailed range the host included of mealybug, test level papaya this (Signoret), parasitoid’s in this longispinus used characterize Pseudococcus species host to process, Mealybug conducted approval specificity. permit were 2006). release al. studies and et importation (Roltsch range USDA-APHIS 1999 of the August in in assist recognized To first was States United continental elbg nptt pot eeepsdt hsprsti o 0dy.Bsdon Based days. 50 10 for each, parasitoid To this other to applied. for exposed clear was results a were study with sprouts honey cup potato which cardboard on 473-mL mealybugs to a within lid prepared plastic was replicate each mealybug, h ikhbsu elbgclueuo which upon culture mealybug hibiscus pink The ragmnswr lomd osn additional send to made also were Arrangements Department US the at quarantine in held was parasitoid the of culture primary The .J otc tal. et Roltsch J. W. A p near sp. . A Allotropa Trin-ozti,osuemealybug, obscure (Targioni-Tozzetti), p near sp. . mecrida Á hnccu solenopsis Phenacoccus 22 X 8 aiiyt eue ntsig stersac utr was culture research the as testing, in used be to facility .Wt h xeto ftssivligtesolenopsis the involving tests of exception the With C. aaocsmarginatus Paracoccus pce,teelf tgswr h otlkl obe to likely most the were stages life these species, Cac 94 ai&Kihaorh 99.In 1989). Krishnamoorthy & Mani 1944; (Clancy mecrida eepeiul etb h quarantine the by sent previously were ise.Teeseiswr selected were species These Tinsley. A p near sp. . ilas&Gaaad Willink, de Granara & Williams A Á 0mayuswr sdper used were mealybugs 30 p near sp. . Á 0scn otidinstar third to second 70 mecrida suoocsviburni Pseudococcus A mecrida p near sp. . a maintained was rmthe from mecrida , Downloaded By: [USDA National Agricultural Library] At: 17:12 27 March 2008 nlddi ahts,cnitn fpn iicsmayu ypssmlryexposed similarly nymphs was mealybug parasitoid, control hibiscus A candidate pink available. the of became consisting to available mealybugs test, not as pre- each were setup in species were and test included replicates all time, status the Because same for counted. the examined health 5 were at parasitoids every replicates emerged apparent all examined and 35, the time were day On last exposure replicates regarding present. all stages designated life recorded 25, and mealybug the to dead) dominant were or of 10 alive day Observations completion as From (designated days. After cartons. for day. the accounted each from were parasitoids removed on of all min days), observations observations (10 setup, Subsequently, 10 period min. initial 90 for of for time made lids the cup were through mealybug At made solenopsis I). were the (Table activity for mealybug parasitoid 20 long-tailed from for ranged replications 46 tested of were to number parasitoids The male two replicate. and a female in two circumstances, these Under replicate. o-epniet h rsneo w ftemayu pce;teppy mealybug papaya the species; mealybug the of mealybug. were two parasitoids solenopsis that of and I). indicated presence (Table exposure under the of species nymphs to mealybug time non-responsive mealybug initial other the the hibiscus of at any Observations pink among evaluation. occur contained not that did reproduction replicates Parasitoid control the of tgs h oqeswr lcdi ador uswt tm xedn hog the waterpiks. through floral extending stems a solenopsis with into cups mealybug and mixed cardboard The with in bottom terminals placed fruit. three were of bouquets consisting The squash hibiscus stages. of ( or bouquets on tested hibiscus sprouts were on potato solenopsis on reared the for reared was except evaluation, been mealybug under species had mealybug All mealybug, 2002. April to 2001 aaiod eetse nmayusmitie nptt sprouts, potato on maintained mealybugs on tested were parasitoids a h nymayu ntesuyta a utbefrtecmlto of completion the for suitable was that study the in mealybug hibiscus mealybug pink of The only development encapsulation. of or Specimens the level species. eggs higher test parasitoid was the this for for of examined replicates account not in may were Female observed This frequently feeding observed. occasions. or mealybugs numerous was feeding moribund host on feeding observed honeydew were host host and mealybug on or obscure the probing probe did ovipositor parasitoids no however, mealybugs; otrnesuisb uhmasgetycmlctsadlmt h niepoesof process entire the limits and complicates western conduct greatly to and means experience, southwestern such our the by From Delaware. studies in in range insectaries facility host pursued quarantine off-site was the from to effort process of species States coordinating costly United of a a cultures time), shipment (typically personnel multiple the available and in not maintain space was facility evaluation simultaneously including for when to location one opportunity at Murray & mealybugs the Driesche (Van Because approaches include presentation & testing 2004). other range Drieshe several host Van and for 2001; choice evaluation Methodologies (Hopper no-choice, I). the (Table published on 2005) been Delfosse and have 2004; testing, effects Reardon monitoring range non-target field of host analysis control 2006). 2004, and biological al. of insect et for (Roltsch fall methodologies occur the not did over through establishment of 2003 that release indicated the spring a Following 2005 from at through released. reared and parasitoids been California subsequently 000 Centro, has 300 El parasitoid in The insectary 2002. of CDFA November on granted was naeaeo 75frtgnrto (F1) generation first 17.5 of average An nteyassneorsuiswr odce,rvesaddsusoson discussions and reviews conducted, were studies our since years the In A. p near sp. Allotropa mecrida. A p near sp. . p near sp. SAAHSiprainadrlaepermit release and importation USDA-APHIS A iicsrosa-sinensis Hibiscus mecrida A. mecrida p near sp. l et eernfo December from run were tests All . otrnetssfor tests range Host aaiod retdt longtailed to oriented parasitoids mecrida ..Weesalother all Whereas L.). eepoue neach in produced were lorp 979 Allotropa P . solenopsis Downloaded By: [USDA National Agricultural Library] At: 17:12 27 March 2008 * Phenacoccus Paracoccus Pseudococcus ofe E an M ete J ilR,MlkrKee .20.Mayusi aionavineyards. California in Mealybugs 2002. R. Malakar-Kuenen RJ, Gill RJ, Bentley KM, Daane KE, Godfrey Pseudococcus efseE.20.Rs n tisi ilgclcnrl ilgclCnrl35:319 Control Biological control. biological in ethics and Risk 2005. ES. Delfosse Mealybug Maconellicoccus lnyD.14.Booyof Biology 1944. DW. Clancy the of maintaining efforts in The TX, testing. Edinburg, USDA-APHIS, for of USDA-APHIS, Wood, culture species parent Leeda Andress, mealybug and Earl providing Sheetz and repeatedly Russel California, for of CA, University Brawley, Daane, Kent thank We Acknowledgements tests such where facility the at evaluation, conducted. under cultures, be highly species multiple will is maintaining enemy financial It simultaneously natural to which present. the devoted in including simultaneously be tests, circumstances be can under choice resources to conducted facility host hosts be and conduct potential testing to another. host the one that opportunity of with recommended successfully no most structure) one or essentially age to For all and experienced densities requiring testing. we difficult regarding result, therefore for (i.e. and was a phase time individuals one As of desired different it out at by the be reared to anticipate, species were at likely locations species cultured remote could at mealybug the cultures one of reason, of arrival that most Despite the tests. cooperation or coordinate necessary completing all best in bottleneck maintain the impassible higher to nearly significantly a a support creates for location, approach financial this of substituting words, level other In testing. range host 980 References policy. Agriculture of employees. Department Federal US US reflect by necessarily co-authored not was does article it The However, appreciated. greatly also was O,dvlpetaae,anra ubro oiudo ednmh n adults. and nymphs dead or moribund of number abnormal abated, development DOA, solenopsis marginatus viburni longispinus species hirsutus nvriyo aiona iiino giutr n aua eore,Pbiain262Okad CA. Oakland, 21612 Publication Resources, Natural and Agriculture of Division California, of University giutrlRsac 69:159 Research Agricultural .J otc tal. et Roltsch J. W. o of No. reps. 0100 20 95 22 50 29 120 31 620 46 75 22 50 27 Allotropa al .Hs ag etifrainadresults. and information test range Host I. Table o fmealybugs of No. lorp burrelli Allotropa Á e rep. per p near sp. 167. terminals) hibiscus (on Á Á Á 022 50 022 50 200 mecrida rgrosprst of parasite gregarious a , eprtr elbglf stages life Mealybug Temperature 65 65 65 65 65 20 nclueadmkn hpet sneeded as shipments making and culture in Á Á Á Á Á Á Á Á Á Á 0 RH 80% 0 RH 80% 0 RH 80% 0 RH 80% 0 RH 80% 22 24 24 24 24 8 8 8 8 8 C C C C C dlspresent adults to nymphs Variable: as itemortality little sacs, ovi- Predominantly radults or nymphs with reps. remaining DOA*, many with reps. 13 itemortality little ovisacs, and Adults mortality little ovisacs, and adults Nymphs, suoocscomstocki Pseudococcus tdy25 day at Á 329. SD 17.5 Mean Rne2 (Range 1parasitoids F1 9 e rep. per ora of Journal . 7.8 0 0 0 0 Á 33) Downloaded By: [USDA National Agricultural Library] At: 17:12 27 March 2008 a receR,RadnR dtr.20.Assighs agsfrprstisadpeaosue for used predators and parasitoids for ranges host Assessing 2004. editors. R, Reardon RG, Driesche Van a receR,Mra J 04 vriwo etn cee n ein sdt siaehs ranges. host estimate to used designs and schemes testing of Overview 2004. TJ. Murray RG, Driesche Van otc J eedr E akni ,AdesE,CreaK 06 ilgclcnrlo h pink the of control Biological 2006. K. Carrera ER, Andress R, Warkentin DE, Meyerdirk WJ, Roltsch ilrD ilR,Wlim J 94 aooi nlssof analysis Taxonomic 1984. grape of DJ. susceptibility Williams pesticide RJ, and suitability Gill stage D, host Miller cycle, Life 1989. A. Krishnamoorthy M, Mani oprK.20.Rsac ed ocrignntre mat fbooia oto nrdcin.In: introductions. control biological of impacts non-target concerning needs Research 2001. KR. Hopper Gonza lsia ilgclcnrl ud obs rcie HE-040.Mratw,W:UD Forest 243. USDA p WV: Team. Morgantown, Enterprise FHTET-2004-03. Technology practice. Health best Forest to Service, guide a control: biological classical n a receR,RadnR dtr.Assighs agsfrprstisadpeaosue for used Forest 68 predators USDA pp WV: Team. and Morgantown, parasitoids Enterprise FHTET-2004-03. Technology practice. for Health best ranges Forest to host Service, guide Assessing a editors. control: R, biological Reardon classical RG, Driesche Van In: oto 37:155 Control mealybug, 86:703 Washington hibiscus of Society Entomological the of Proceedings Pseudococcidae). (Homoptera yoy of synonym parasitoid, mealybug anegE ct K umyP,eios vlaigidrc clgclefcso ilgclcontrol. biological of effects ecological 39 indirect pp Evaluating Publishing. editors. CABI PC, UK: Quimby Wallingford, JK, Scott E, Wajnberg uvyfrpn iicsmealybug, hibiscus pink for survey ooc.Eyta ora fBooia etCnrl13:1 Control Pest Biological of Journal Egyptian Morocco. lzD lHniyA,MuaS,Tipty V dyD raiznV,MyrikD.20.A 2003. DE. Meyerdirk VA, Trjapitzin D, Adly SV, Triapitsyn SM, Mousa AH, El-Heneidy D, ´lez suoocsobscurus Pseudococcus Á 166. aoelccu hirsutus Maconellicoccus lorp japonica Allotropa aoelccu hirsutus Maconellicoccus si,adacmaio with comparison a and Essig, pn ora fBooia oto 3:7 Control Biological of Journal sp.n. Á 56. Gen,i h o-eetrgo fClfri.Biological California. of region low-desert the in (Green), Gen n t aaiod nEyt pi and Spain Egypt, in parasitoids its and (Green) Á Á 5. 89. otrnetssfor tests range Host suoocsaffinis Pseudococcus suoocsmaritimus Pseudococcus Á 9. Msel.Asenior A (Maskell). lorp 981 Allotropa (Ehrhorn) Á 713.