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United States Patent (19) 11 Patent Number: 6,127,166 Bayley Et Al

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United States Patent (19) 11 Patent Number: 6,127,166 Bayley Et Al USOO6127166A United States Patent (19) 11 Patent Number: 6,127,166 Bayley et al. (45) Date of Patent: Oct. 3, 2000 54 MOLLUSCAN LIGAMENT POLYPEPTIDES Dickinson et al., (1995), Muscle Efficiency and Elastic AND GENES ENCODING THEM Storage in the Flight Motor of Drosophila, Science, 268:87-90. 76 Inventors: Hagan Bayley, 1800 Springbrook Engel (1997), Versatile Collagens in Invertebrates, Science Estates Dr., College Station, Tex. 277: 1785-1786. 77845; Qiuping Cao, 15 Sandpiper Dr., Erickson (1997), Stretching Single Protein Molecules: Titin Shrewsbury, Mass. 01545; Yunjuan is a Weird Spring, Science 276:1090–1092. Wang, 4300 Boyett St., Bryan, Tex. Guerette et al. (1996), Silk Properties Determined by Gland 778O1 Specific Expression of a Spider Fibroin Gene Family, Sci ence 272:112-114. 21 Appl. No.: 08/963,168 Hare (1963), Amino Acids in the Proteins from Aragonite and Calcite in the Shells of Mytilus Californianus, Science 22 Filed: Nov. 3, 1997 139:216-217. 51 Int. Cl." ............................. C12N 1/20; C12N 15/00; Hinman et al. (1992), Isolation of a Clone Encoding a C12P 21/06; CO7H 21/02 Second Dragline Silk Fibroin, J. Biol. Chem. 52 U.S. Cl. .................. 435/252.3; 435/69.1; 435/320.1; 267:19320-19324. 435/325; 536/23.1; 536/23.5 Kahler et al. (1976), The Chemical Composition and 58 Field of Search ................................ 435/69.1, 320.1, Mechanical Properties of the Hinge Ligament in Bivalve 435/325, 252.3; 536/23.1, 23.5 Molluscs, Biol. Bull. 151:161-181. Keller (1997), Muscle Structure: Molecular Bungees, 56) References Cited Nature 387:233-235. U.S. PATENT DOCUMENTS Kellermeyer et al. 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Wrigley (1996), Giant Proteins with Flour Power, Nature News Dec. 19. 1994, 40–51. 381:738-739. 6,127,166 1 2 MOLLUSCAN LIGAMENT POLYPEPTIDES includes, for example, a recombinant DNA which is incor AND GENES ENCODING THEM porated into a vector, into an autonomously replicating plasmid or virus, or into the genomic DNA of a prokaryote This invention was made in part with government Sup or eukaryote, or which exists as a separate molecule (e.g., a port under grant number DAAHO4-94-2-0002 awarded by cDNA or a genomic or cDNA fragment produced by poly the Army Research Office. The United States government merase chain reaction (PCR) or restriction endonuclease may have certain rights in the invention. digestion) independent
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