DOCSLIB.ORG

Towards a Better Understanding of the Molecular Mechanisms Underlying Plant Development and Stress Response

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Towards a Better Understanding of the Molecular Mechanisms Underlying Plant Development and Stress Response Clemson University TigerPrints All Dissertations Dissertations December 2017 Towards a Better Understanding of the Molecular Mechanisms Underlying Plant Development and Stress Response Peipei Wu Clemson University, [email protected] Follow this and additional works at: https://tigerprints.clemson.edu/all_dissertations Recommended Citation Wu, Peipei, "Towards a Better Understanding of the Molecular Mechanisms Underlying Plant Development and Stress Response" (2017). All Dissertations. 2548. https://tigerprints.clemson.edu/all_dissertations/2548 This Dissertation is brought to you for free and open access by the Dissertations at TigerPrints. It has been accepted for inclusion in All Dissertations by an authorized administrator of TigerPrints. For more information, please contact [email protected]. TOWARDS A BETTER UNDERSTANDING OF THE MOLECULAR MECHANISMS UNDERLYING PLANT DEVELOPMENT AND STRESS RESPONSE A Dissertation Presented to the Graduate School of Clemson University In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy Biochemistry and Molecular Biology by Peipei Wu December 2017 Accepted by: Dr. Hong Luo, Committee Chair Dr. Julia Frugoli Dr. Rajandeep Sekhon Dr. Liangjiang Wang ` ABSTRACT The spectacular array of diverse plant forms as well as the predominantly sessile life style of plants raises two questions that have been fascinating to scientists in the field of plant biology for many years: 1) how do plants develop to a specific size and shape? 2) how do plants respond to environmental stresses given its immobility? Plant organ development to a specific size and shape is controlled by cell proliferation and cell expansion. While the cell proliferation process is extensively studied, the cell expansion process remains largely unknown, and can be affected by several factors, such as cell wall remodeling and the incorporation of new wall materials. To better understand the genetic basis of plant development, we identified an Arabidopsis T-DNA insertion mutant named development related Myb-like 1 (drmy1), which showed altered size and shape in both vegetative and reproductive organs due to defective cell expansion. We further demonstrated that the defective cell expansion in the drmy1 mutant is linked to the change in cell wall composition. Complementation testing by introduction of DRMY1 into the mutant background rescued the phenotype, indicating that DRMY1 is a functional regulator of plant organ development. The DRMY1 protein contains a single Myb-like DNA binding domain and is localized in the nucleus, and may cooperate with other transcription factors to regulate downstream gene expression as DRMY1 itself does not possess transactivation ability. DRMY1 expression analysis revealed that its expression is reduced by the plant hormone ethylene (a negative regulator of cell expansion) while induced by ABA (a positive regulator of cell expansion). Furthermore, whole transcriptome profiling suggested that DRMY1 might control cell expansion directly by regulating genes ii ` related to cell wall biosynthesis/remodeling and ribosome biogenesis or indirectly through regulating genes involved in ethylene and ABA signaling pathways. Plants cannot “escape” from salinity stress but have evolved different mechanisms for salt tolerance over the time of adaptation to salinity. About 1% of plant species named halophytes can survive and thrive in environments containing high salt concentrations, which makes it important to understand their salt tolerance mechanisms and the responsible genes. Here, we investigated salt tolerance mechanisms in Supreme, the most salt-tolerant cultivar of a halophytic warm-seasoned perennial grass, Seashore paspalum (Paspalum vaginatum) at the physiological and transcriptomic levels by comparative study with another cultivar Parish, which possesses moderate salinity tolerance. Our results suggest that Na+ accumulation under normal conditions and further increased accumulation under high salinity conditions (400 mM NaCl), possibly by vacuolar sequestration is a crucial mechanism for salinity tolerance in Supreme. Our data suggests that Na+ accumulation in Supreme under normal conditions might trigger the secondary messenger Ca2+ for signal transduction and the resulting upregulation of salt stress related transcription factors in addition to serving as cheap osmolytes to facilitate water uptake. Moreover, the retention of K+ under salt treatment, which can counteract the toxicity of Na+, is a protective mechanism for both cultivars. A strong oxidation-reduction process and nucleic acid binding activity under high salinity conditions are two other contributors to the salinity tolerance in both cultivars. We also identified ion transporters including NHXs and H+- PPases for Na+ sequestration and K+ uptake transporters, which can be used as candidate iii ` genes for functional studies and potential targets to engineer plants for enhanced salinity tolerance, opening new avenues for future research. iv ` DEDICATION I dedicate this dissertation to my parents, Jinlin Lu and Xiuyun Liu, who give me their best love and support. This work is also dedicated to my husband Yijian Qiu, who helped me get through tough times during my PhD study with encouragement and support. v ` ACKNOWLEDGMENTS Firstly, I would like to express my sincere gratitude to my advisor Dr. Hong Luo for his invaluable guidance and enormous support for my PhD study. I also greatly appreciate my committee members Dr. Julia Frugoli, Dr. Liangjiang Wang, and Dr. Rajandeep Sekhon for their insightful and helpful suggestions on my projects. I would also like to thank my current and previous lab members, Qian Hu, Dr. Zhigang Li, Dr. Ning Yuan and Dr. Zhihui Chang for their generous help and support. I’m also very grateful for the following people: Dr. Steven Cogill (Stanford University), Yijian Qiu (Clemson University), Dr. Christopher Saski (CUGI), Dr. Rooksana Noorai (CUGI), Xiaoxia Xia (CUGI), Dr. Vijay Shankar (CUGI), Wei Li (Clemson University) for their help in RNAseq data analysis; Computing experts Dr. Marcin Ziolkowski, Dr. Linh Ngo, Ashwin Srinath, Jeff Denton from Clemson University Computing and Information Technology (CCIT) for their help in programming and troubleshooting; Dr. Rhonda Reigers Powell from Clemson Light Imaging Facility (CLIF) for her guidance in fluorescence imaging; Dr. Cliff Foster from Michigan State University for his help with cell wall composition analysis; Dr. Cora MacAlister from University of Michigan and Dr. David Jackson from Cold Spring Harbor for providing Lat52B-GUS transgenic Arabidopsis seeds and protocols; Dr. Dayong Li from Chinese Academy of Sciences for providing yeast systems for transactivation assay; Dr. Paul Raymer from University of Georgia for providing Seashore paspalum cultivars. vi ` TABLE OF CONTENTS Page TITLE PAGE .................................................................................................................... i ABSTRACT ..................................................................................................................... ii DEDICATION ................................................................................................................. v ACKNOWLEDGMENTS .............................................................................................. vi LIST OF TABLES ......................................................................................................... ix LIST OF FIGURES ......................................................................................................... x CHAPTER ONE LITERATURE REVIEW AND RESEARCH OBJECTIVES ...................... 1 Part I: The Regulation of Plant Development Cell Proliferation ........................................................................................ 2 Cytoplasmic Growth .................................................................................. 7 Endocycle ................................................................................................... 8 Cell Expansion ......................................................................................... 11 Compensation Mechanism: Organ-Wide Regulation .............................. 15 Part II: The Regulation of Plant Salt Stress Response Na+ Transporters and Plant Salt Tolerance .............................................. 18 Compatible Solutes and Osmotic Adjustment ......................................... 21 Antioxidative Defense Mechanism .......................................................... 22 Salt Signaling and Regulatory Pathways ................................................. 22 Salt Tolerance Mechanisms in Halophytes .............................................. 28 Part III: The Objectives of the Current Research ..................................... 30 TWO DRMY1, A NOVEL MYB-LIKE TRANSCRIPTION FACTOR REGULATES CELL EXPANSION DURING PLANT DEVELOPMENT AND AFFECTS SEED PRODUCTION IN ARABIDOPSIS ............................................................................... 31 Abstract .................................................................................................... 32 Introduction .............................................................................................. 32 Methods.................................................................................................... 37 vii ` Results ...................................................................................................... 45 Discussion ...............................................................................................
Load more

Recommended publications
  • Transforming Undergraduate Education for Future Research Biologists
    THE NATIONAL ACADEMIES PRESS This PDF is available at http://nap.edu/10497 SHARE BIO2010: Transforming Undergraduate Education for Future Research Biologists DETAILS 208 pages | 6 x 9 | PAPERBACK ISBN 978-0-309-08535-9 | DOI 10.17226/10497 AUTHORS BUY THIS BOOK Committee on Undergraduate Biology Education to Prepare Research Scientists for the 21st Century, National Research Council FIND RELATED TITLES Visit the National Academies Press at NAP.edu and login or register to get: – Access to free PDF downloads of thousands of scientific reports – 10% off the price of print titles – Email or social media notifications of new titles related to your interests – Special offers and discounts Distribution, posting, or copying of this PDF is strictly prohibited without written permission of the National Academies Press. (Request Permission) Unless otherwise indicated, all materials in this PDF are copyrighted by the National Academy of Sciences. Copyright © National Academy of Sciences. All rights reserved. BIO2010: Transforming Undergraduate Education for Future Research Biologists Committee on Undergraduate Biology Education to Prepare Research Scientists for the 21st Century Board on Life Sciences Division on Earth and Life Studies THE NATIONAL ACADEMIES PRESS Washington, D.C. www.nap.edu Copyright National Academy of Sciences. All rights reserved. BIO2010: Transforming Undergraduate Education for Future Research Biologists THE NATIONAL ACADEMIES PRESS 500 Fifth Street, N.W. Washington, DC 20001 NOTICE: The project that is the subject of this report was approved by the Governing Board of the National Research Council, whose members are drawn from the councils of the National Academy of Sciences, the National Academy of Engineering, and the Institute of Medicine.
    [Show full text]
  • Looking at Earth: an Astronaut's Journey Induction Ceremony 2017
    american academy of arts & sciences winter 2018 www.amacad.org Bulletin vol. lxxi, no. 2 Induction Ceremony 2017 Class Speakers: Jane Mayer, Ursula Burns, James P. Allison, Heather K. Gerken, and Gerald Chan Annual David M. Rubenstein Lecture Looking at Earth: An Astronaut’s Journey David M. Rubenstein and Kathryn D. Sullivan ALSO: How Are Humans Different from Other Great Apes?–Ajit Varki, Pascal Gagneux, and Fred H. Gage Advancing Higher Education in America–Monica Lozano, Robert J. Birgeneau, Bob Jacobsen, and Michael S. McPherson Redistricting and Representation–Patti B. Saris, Gary King, Jamal Greene, and Moon Duchin noteworthy Select Prizes and Andrea Bertozzi (University of James R. Downing (St. Jude Chil- Barbara Grosz (Harvard Univer- California, Los Angeles) was se- dren’s Research Hospital) was sity) is the recipient of the Life- Awards to Members lected as a 2017 Simons Investi- awarded the 2017 E. Donnall time Achievement Award of the gator by the Simons Foundation. Thomas Lecture and Prize by the Association for Computational American Society of Hematology. Linguistics. Nobel Prize in Chemistry, Clara D. Bloomfield (Ohio State 2017 University) is the recipient of the Carol Dweck (Stanford Univer- Christopher Hacon (University 2017 Robert A. Kyle Award for sity) was awarded the inaugural of Utah) was awarded the Break- Joachim Frank (Columbia Univer- Outstanding Clinician-Scientist, Yidan Prize. through Prize in Mathematics. sity) presented by the Mayo Clinic Di- vision of Hematology. Felton Earls (Harvard Univer- Naomi Halas (Rice University) sity) is the recipient of the 2018 was awarded the 2018 Julius Ed- Nobel Prize in Economic Emmanuel J.
    [Show full text]
  • Medical Advisory Board September 1, 2006–August 31, 2007
    hoWard hughes medical iNstitute 2007 annual report What’s Next h o W ard hughes medical i 4000 oNes Bridge road chevy chase, marylaNd 20815-6789 www.hhmi.org N stitute 2007 a nn ual report What’s Next Letter from the president 2 The primary purpose and objective of the conversation: wiLLiam r. Lummis 6 Howard Hughes Medical Institute shall be the promotion of human knowledge within the CREDITS thiNkiNg field of the basic sciences (principally the field of like medical research and education) and the a scieNtist 8 effective application thereof for the benefit of mankind. Page 1 Page 25 Page 43 Page 50 seeiNg Illustration by Riccardo Vecchio Südhof: Paul Fetters; Fuchs: Janelia Farm lab: © Photography Neurotoxin (Brunger & Chapman): Page 3 Matthew Septimus; SCNT images: by Brad Feinknopf; First level of Rongsheng Jin and Axel Brunger; iN Bruce Weller Blake Porch and Chris Vargas/HHMI lab building: © Photography by Shadlen: Paul Fetters; Mouse Page 6 Page 26 Brad Feinknopf (Tsai): Li-Huei Tsai; Zoghbi: Agapito NeW Illustration by Riccardo Vecchio Arabidopsis: Laboratory of Joanne Page 44 Sanchez/Baylor College 14 Page 8 Chory; Chory: Courtesy of Salk Janelia Farm guest housing: © Jeff Page 51 Ways Illustration by Riccardo Vecchio Institute Goldberg/Esto; Dudman: Matthew Szostak: Mark Wilson; Evans: Fred Page 10 Page 27 Septimus; Lee: Oliver Wien; Greaves/PR Newswire, © HHMI; Mello: Erika Larsen; Hannon: Zack Rosenthal: Paul Fetters; Students: Leonardo: Paul Fetters; Riddiford: Steitz: Harold Shapiro; Lefkowitz: capacity Seckler/AP, © HHMI; Lowe: Zack Paul Fetters; Map: Reprinted by Paul Fetters; Truman: Paul Fetters Stewart Waller/PR Newswire, Seckler/AP, © HHMI permission from Macmillan Page 46 © HHMI for Page 12 Publishers, Ltd.: Nature vol.
    [Show full text]
  • Ingredients for Success 18Th HFSP Awardees Meeting
    Ingredients for success In 2018, we yet again embarked on a new era in the HFSP by Guntram Bauer Fellowship program. After careful internal discussion and HFSPO Director of Scientific Affairs and Communications preparations, the review committee was presided over by a non- reviewing chair. Peter Koopman from the Institute of Molecular Bioscience of the University of Queensland took center stage as chair of this committee. Peter finished a four year tenure as a member of the fellowship review committee in 2016 and arrived back in Strasbourg after a three month sailing trip in the southern Pacific. Peer review at HFSP is not reading tea leaves but serious and demanding work for all involved. Thanks to Peter Koopman we successfully completed the first fellowship review committee run by a non-reviewing chair. We are still not sure what the magic ingredient was that made this fellowship committee stand out. Whether it was a truly committed international review panel, or the drive and oversight of its non-reviewing chair, or simply because of the HFSP Sauce which arrived in the chair's suitcase in Strasbourg. Probably all three, though be assured that we will get to the bottom of the HFSP Sauce! I don’t think we are boasting about HFSP when we say that our Program always tries to synchronize its procedures with the dynamics at the frontiers of science. This is particularly true for our peer review process. Over many years we have made more than just subtle changes to our procedures in response to feedback from the scientific community.
    [Show full text]
  • Annual Report Fy 2018 Human Frontier Science Program Organization
    APRIL 2017 APRIL 2018 — MARCH 2019 ANNUAL REPORT FY 2018 HUMAN FRONTIER SCIENCE PROGRAM ORGANIZATION The Human Frontier Science Program Organization (HFSPO) is unique, supporting international collaboration to undertake innovative, risky, basic research at the frontier of the life sciences. Special emphasis is given to the support and training of independent young investigators, beginning at the postdoctoral level. The Program is implemented by an international organisation, supported financially by Australia, Canada, France, Germany, India, Italy, Japan, the Republic of Korea, New Zealand, Norway, Singapore, Switzerland, the United Kingdom of Great Britain and Nothern Ireland, the United States of America, and the European Commission. Since 1990, over 7000 researchers from more than 70 countries have been supported. Of these, 28 HFSP awardees have gone on to receive the Nobel Prize. 2 The following documents are available on the HFSP website www.hfsp.org: Joint Communiqués (Tokyo 1992, Washington 1997, Berlin 2002, Bern 2004, Ottawa 2007, Canberra 2010, Brussels 2013, London 2016): https://www.hfsp.org/about/governance/membership Statutes of the International Human Frontier Science Program Organization: https://www.hfsp.org/about/governance/hfspo-statutes Guidelines for the participation of new members in HFSPO: https://www.hfsp.org/about/governance/membership General reviews of the HFSP (1996, 2001, 2006-2007, 2010, 2018): https://www.hfsp.org/about/strategy/reviews Updated and previous lists of awards, including titles and abstracts:
    [Show full text]
  • 2013 Photosynthetic Systems Research Meeting
    2013 Photosynthetic Systems Research Meeting Westin Annapolis Hotel Annapolis, MD November 3-6, 2013 Office of Basic Energy Sciences Chemical Sciences, Geosciences & Biosciences Division 2013 Photosynthetic Systems Research Meeting Program and Abstracts Westin Annapolis Hotel Annapolis, MD November 3-6, 2013 Chemical Sciences, Geosciences, and Biosciences Division Office of Basic Energy Sciences Office of Science U.S. Department of Energy i Cover art is taken from the public domain and can be found at: http://all-free-download.com/free-photos/leaves_green_back_light_230944.html The research grants and contracts described in this document are, unless specifically labeled otherwise, supported by the U.S. DOE Office of Science, Office of Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division. DISCLAIMER This report is a compilation of accounts of work sponsored by an agency of the United States Government. Neither the United States government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise, does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. ii Foreword This volume provides a record of the third biennial meeting of the Principal Investigators (PIs) funded by the Photosynthetic Systems program and is sponsored by the Chemical Sciences, Geosciences, and Biosciences Division of the Office of Basic Energy Sciences (BES) in the U.S.
    [Show full text]
  • Pnas11052ackreviewers 5098..5136
    Acknowledgment of Reviewers, 2013 The PNAS editors would like to thank all the individuals who dedicated their considerable time and expertise to the journal by serving as reviewers in 2013. Their generous contribution is deeply appreciated. A Harald Ade Takaaki Akaike Heather Allen Ariel Amir Scott Aaronson Karen Adelman Katerina Akassoglou Icarus Allen Ido Amit Stuart Aaronson Zach Adelman Arne Akbar John Allen Angelika Amon Adam Abate Pia Adelroth Erol Akcay Karen Allen Hubert Amrein Abul Abbas David Adelson Mark Akeson Lisa Allen Serge Amselem Tarek Abbas Alan Aderem Anna Akhmanova Nicola Allen Derk Amsen Jonathan Abbatt Neil Adger Shizuo Akira Paul Allen Esther Amstad Shahal Abbo Noam Adir Ramesh Akkina Philip Allen I. Jonathan Amster Patrick Abbot Jess Adkins Klaus Aktories Toby Allen Ronald Amundson Albert Abbott Elizabeth Adkins-Regan Muhammad Alam James Allison Katrin Amunts Geoff Abbott Roee Admon Eric Alani Mead Allison Myron Amusia Larry Abbott Walter Adriani Pietro Alano Isabel Allona Gynheung An Nicholas Abbott Ruedi Aebersold Cedric Alaux Robin Allshire Zhiqiang An Rasha Abdel Rahman Ueli Aebi Maher Alayyoubi Abigail Allwood Ranjit Anand Zalfa Abdel-Malek Martin Aeschlimann Richard Alba Julian Allwood Beau Ances Minori Abe Ruslan Afasizhev Salim Al-Babili Eric Alm David Andelman Kathryn Abel Markus Affolter Salvatore Albani Benjamin Alman John Anderies Asa Abeliovich Dritan Agalliu Silas Alben Steven Almo Gregor Anderluh John Aber David Agard Mark Alber Douglas Almond Bogi Andersen Geoff Abers Aneel Aggarwal Reka Albert Genevieve Almouzni George Andersen Rohan Abeyaratne Anurag Agrawal R. Craig Albertson Noga Alon Gregers Andersen Susan Abmayr Arun Agrawal Roy Alcalay Uri Alon Ken Andersen Ehab Abouheif Paul Agris Antonio Alcami Claudio Alonso Olaf Andersen Soman Abraham H.
    [Show full text]
  • 2011 Photosynthetic Systems Research Meeting
    2011 Photosynthetic Systems Research Meeting Baltimore Marriott Waterfront Hotel Baltimore, MD November 6-9, 2011 Office of Basic Energy Sciences Chemical Sciences, Geosciences & Biosciences Division 2011 Photosynthetic Systems Research Meeting Program and Abstracts Baltimore Marriott Waterfront Hotel Baltimore, MD November 6-9, 2011 Chemical Sciences, Geosciences, and Biosciences Division Office of Basic Energy Sciences Office of Science U.S. Department of Energy i Cover art is taken from the public domain and can be found at: http://www.publicdomainpictures.net/view-image.php?image=16623&picture=backlit-leaves. This document was produced under contract number DE-AC05-060R23100 between the U.S. Department of Energy and Oak Ridge Associated Universities. The research grants and contracts described in this document are, unless specifically labeled otherwise, supported by the U.S. DOE Office of Science, Office of Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division. ii Foreword This volume provides a record of the second biennial meeting of the Principal Investigators (PIs) funded by the Photosynthetic Systems program and is sponsored by the Chemical Sciences, Geosciences, and Biosciences Division of the Office of Basic Energy Sciences (BES) in the U.S. Department of Energy (DOE). Within DOE BES, there are two programs that fund basic research in energy-relevant biological sciences, Photosynthetic Systems and Physical Biosciences. These two Biosciences programs, along with a strong program in Solar Photochemistry, comprise the current Photochemistry and Biochemistry Team. This meeting specifically brings together under one roof all of the PIs funded by the Photosynthetic Systems program, along with Program Managers and staff not only from DOE BES but also other offices within DOE, the national labs, and other federal funding agencies.
    [Show full text]
  • Office of Basic Energy Sciences
    DRAFT Office of Basic Energy Sciences Accomplishments FY 1994 DRAFT Major Facilities Construction Highlights Advanced Photon Source Semiannual Review of Advanced Photon Source Proiect (APS) A Construction Project Management Review of the APS Project was conducted at Argonne National Laboratory on November 15-17, 1994 by the Office of Basic Energy Sciences and the Office of Management. The Review Committee consisted of Mr. James Carney, Construction Management Support Division, Chairman; Robert A. Zich, Acquisition and Assistance Management Division; Office of Basic Energy Sciences' Drs. Albert E. Evans, Frederick A. Koomanoff, William T. Oosterhuis, and Walter M. Polansky, together with eleven peer reviewers from DOE Laboratories. Also present were Mr. Matthew Cole from the Office of Environment, Safety, and Health Technical Support, Dr. Bal Mahajan of the Office of Performance Assessment, and Edward F. Koch from the Office of Field Management. The Committee is pleased to report that the APS Project is meeting budget ($811.9 Million Total Project Cost) and time schedules. Construction of the accelerator should be complete by January 1995. All construction, now mostly office and laboratory space, should be completed by February 1996. Commissioning, now underway, should-be complete well in advance of the December 1996 date scheduled for the start of routine operation. Development of Non-Polluting Process for Cleaning Metal Receives Award by the State of Illinois The Advanced Photon Source (APS) scientists, engineers, and technicians have combined their efforts and ideas to develop an alternate process for chemically cleaning aluminum-alloy vacuum chamber sections prior to their installation into the APS. The result of their efforts was-the elimination of 3,000 gallons of hazardous chemical waste per month and an estimated cost savings for waste removal of $300,000 in the first year.
    [Show full text]
  • Download the 2017-18 Annual Report
    Molecular Biology Institute ANNUAL REPORT 2017-2018 (310) 825-1018 mbi.ucla.edu UCLA Boyer Hall [email protected] MOLECULAR BIOLOGY INSTITUTE REMEMBERING PAUL BOYER MBI DIRECTOR’S REPORT 1 MBI COMMITTEES 3 MBI ADMINISTRATIVE SUPPORT STAFF ACTIVITIES Administrative Services 4 Building Management 5 MBI PROGRAMS & EVENTS The David S. Sigman Memorial Lectureship & Symposium 7 The Audree Fowler Fellows in Protein Science 8 The Jules Brenner Achievement Fellowship in Molecular Biology 9 Boyer/Parvin Postdoctoral Research Awards 10 Thursday Seminar Series 11 MBI Interdisciplinary Faculty Seminars 14 MBI Annual Retreat & Research Conference 18 Biotechnology Edge Workshop 16 Focus on Imaging Workshop 17 Mouse Genome Informatics Workshop 18 MBI FACULTY RESEARCH & PROFESSIONAL ACTIVITIES Newly Appointed Members 19 Honors & Professional Awards 22 Service on UCLA Committees 23 Service on External Committees 27 Patents Issued 32 Visiting Faculty & Scholars 33 MBI PARTNERS IN BOYER HALL Institute for Quantitative & Computational Biology (QCBio) 35 The QCBio Collaboratory 35 UCLA-DOE Institute 35 Fermentation Core Facility 36 GRADUATE PROGRAMS Molecular Biology Interdepartmental Ph.D. Program (MBIDP) 37 Whitcome Pre-doctoral Training Program 54 MEMBER PUBLICATIONS MBI Faculty Publications 55 PAUL D. BOYER 1918 - 2018 REMEMBERING WITH GRATITUDE PAUL D. BOYER 1918 - 2018 Dear Colleagues, This year will be forever memorable in the history of our Institute because it marks the passing of our beloved Paul Boyer. His many scientific and academic accomplishments have been highlighted in several journals and it has been wonderful to hear so many colleagues speak about how much Dr. Boyer meant to them. We are grateful for his vision, his collegiality, his commitment to scientific excellence and his optimistic spirit, all of which became part of the culture of our institute.
    [Show full text]
  • UNIVERSITY of CALIFORNIA SAN DIEGO High
    UNIVERSITY OF CALIFORNIA SAN DIEGO High-resolution moleculAr networks from novel ‘omics’ ApproAches elucidAte survival strAtegies in orgAnisms from lAnd to seA A dissertAtion submitted in pArtiAl satisfAction of the requirements for the degree Doctor of Philosophy in Biology by Shelly Ann Trigg Committee in chArge: Professor Joseph Ecker, ChAir Professor JoAnne Chory Professor Theresa GAAsterlAnd Professor Trey Ideker Professor Jose PrunedA-Paz 2018 Copyright Shelly Ann Trigg, 2018 All rights reserved. The DissertAtion of Shelly Ann Trigg is Approved, And is AcceptAble in quAlity And form for publication on microfilm And electronically: _________________________________________________________ _________________________________________________________ ChAir University of CAliforniA San Diego 2018 iii DEDICATION To my brother, Scott MArk WAnAmAker. To my husbAnd Austin Engel Trigg. To my pArents MArk And TwilA WAnAmAker. To my fAmily: BFS, JAS, AC, GEP, DMT, KET, And RMT To my undergrAduAte Advisor JRC, And former professors. To my students. To the American people, that they mAy AlwAys hAve eAsy Access to current And pAst publicly funded scientific reseArch reports. iv EPIGRAPH Study the science of Art. Study the Art of science. Develop your senses - especiAlly leArn how to see. ReAlize thAt everything connects to everything else. Principles for the Development of a Complete Mind v TABLE OF CONTENTS SignAture PAge………………………………………………… iii DedicAtion………………………………………………………. iv EpigrAph………………………………………………………… v Table of Contents……………………………………………… vi List of SupplementAl Files………………..…………………… vii List of Figures And TAbles…………………………………….. viii Acknowledgements……………………………………………. x VitA………………………………………………………………. xii AbstrAct of the DissertAtion…………………………………… xvii ChApter 1: Next-generAtion modifications of the yeAst two-hybrid system to Advance protein-protein interAction dAtA collection………………………………………………….. 1 ChApter 2: CrY2H-seq: A mAssively multiplexed Assay for deep-coverAge interActome mApping…………………….…..
    [Show full text]
  • CV Joanne Chory
    Curriculum Vitae Prof. Dr. Joanne Chory Name: Joanne Chory Born: 19 March 1955 Major Scientific Interests: Adaptation processes, plant growth, genetic diversity, natural variations, light conditions, shade avoidance syndrome, plant biology, climate change, growth hormone auxin, phytochromes, brassinosteroids Joanne Chory is an American plant biologist whose work has contributed to the identification of plant hormones. She investigates the natural variations in plant species and plant reactions to different light and temperature conditions. Her studies have helped us understand how plants adapt to climate change. Academic and Professional Career since 1999 Adjunct Professor, Biology Department, University of California, San Diego, USA since 1998 Director, Plant Biology Laboratory, The Salk Institute, La Jolla, USA 1997 Investigator, The Howard Hughes Medical Institute. USA 1994 ‐ 1998 Associate Professor, Plant Biology Laboratory, The Salk Institute, La Jolla, USA 1992 ‐ 1994 Adjunct Assistant Professor, Biology Department, University of California, San Diego, USA 1988 ‐ 1994 Assistant Professor, Plant Biology Laboratory, The Salk Institute, USA 1984 ‐ 1988 Postdoctoral Fellow, Harvard Medical School, USA Ph.D. in microbiology, University of Illinois, USA B.A. in biology, Oberlin College, Ohio, USA Nationale Akademie der Wissenschaften Leopoldina www.leopoldina.org 1 Honours and Awarded Memberships 2018 Breakthrough Prize in Life Sciences 2012 Genetics Society of America Medal 2011 Foreign Member of the Royal Society 2009 Foreign Member
    [Show full text]