Morphological and Genetic Evidence for Multiple Evolutionary Distinct
Morphological and Genetic Evidence for Multiple Evolutionary Distinct Lineages in the Endangered and Commercially Exploited Red Lined Torpedo Barbs Endemic to the Western Ghats of India Lijo John1,2., Siby Philip3,4,5., Neelesh Dahanukar6,7, Palakkaparambil Hamsa Anvar Ali5, Josin Tharian8, Rajeev Raghavan5,7,9,10*, Agostinho Antunes3,4* 1 Marine Biotechnology Division, Central Marine Fisheries Research Institute (CMFRI), Kochi, India, 2 Export Inspection Agency (EIA), Kochi, India, 3 CIMAR/CIIMAR, Centro Interdisciplinar de Investigac¸a˜o Marinha e Ambiental, Rua dos Bragas, Porto, Portugal, 4 Departamento de Biologia, Faculdade de Cieˆncias, Universidade do Porto, Rua do Campo Alegre, Porto, Portugal, 5 Conservation Research Group (CRG), St. Albert’s College, Kochi, India, 6 Indian Institute of Science Education and Research (IISER), Pune, India, 7 Zoo Outreach Organization (ZOO), Coimbatore, India, 8 Department of Zoology, St. John’s College, Anchal, Kerala, India, 9 Durrell Institute of Conservation and Ecology (DICE), University of Kent, Canterbury, United Kingdom, 10 Research Group Zoology: Biodiversity & Toxicology, Center for Environmental Sciences, University of Hasselt, Diepenbeek, Belgium Abstract Red lined torpedo barbs (RLTBs) (Cyprinidae: Puntius) endemic to the Western Ghats Hotspot of India, are popular and highly priced freshwater aquarium fishes. Two decades of indiscriminate exploitation for the pet trade, restricted range, fragmented populations and continuing decline in quality of habitats has resulted in their ‘Endangered’ listing. Here, we tested whether the isolated RLTB populations demonstrated considerable variation qualifying to be considered as distinct conservation targets. Multivariate morphometric analysis using 24 size-adjusted characters delineated all allopatric populations. Similarly, the species-tree highlighted a phylogeny with 12 distinct RLTB lineages corresponding to each of the different riverine populations.
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