Thermo Scientific DXR Raman Microscope

Product Specifications

for conﬁdence in sample identiﬁcation true point and shoot Raman spectroscopy depth resolution reproducible laser power at sample Olympus microscope components Rapid, automated, multi-point calibration Patented autoexposure and autofocus for 1 µm x, y spatial resolution and 2 µm Laser Power Regulator to assure confocal design True Superb visual quality Compatible with a full range of high-quality • • • • • • •

Designed for versatility, the DXR Raman Designed for versatility, optimal results with demanding samples highest optimal performance non-restricted environments Class I laser-safe design for use in Class I laser-safe Up to three excitation wavelengths for Patented autoalignment system for required in demanding analytical environments. microscope is designed to produce designed is microscope The DXR Raman microscope delivers excep- tional spatial resolution, together with the and reproducibility performance reliability, microscope makes it as easy as possible to get high quality results. • • • The Thermo Scientific DXR Raman DXR Scientific The Thermo Raman by demanded the results the ease required experts with laboratories. by busy analytical optical components SmartLock autoalignment and a patented fast and easy, system make set-up calibration, precise. Automated ensure quality focus and exposure measurement. results with every

the 1-micron level For sample microanalysis at or below at microanalysis sample For DXR Raman Microscope Microscope Raman DXR Thermo Scientific Part of Thermo Fisher Scientific molecular spectroscopy molecular -1 FWHM 3 -1 -1 3300 cm 50 cm serial number, serial number, ™ standard deviation with -1 -1 -1 -1 < 1 µm ± 2 cm full-range grating FWHM 6.0 cm FWHM -1 -1 -1 -1 cessory specifications. 50 cm (high brightness)3300 cm (high power) 50 cm 1800 cm andwidth. Lasers -1 -1 FWHM 5.0 cm FWHM 2 cm -1 -1 -1 -1 50 cm 3500 cm 50 cm 1800 cm . -1 -1 -1 -1 FWHM 5.0 cm FWHM FWHM 2 cm -1 -1 -1 -1 -1 -1 50 cm 50 cm Resolution with low-resolution motorized stageResolution with high-precision motorized stage532 nm laser 3 µm 633 nm laser µm, diffraction-limited 1 780 nm high brightness laser axisWavenumber 750:1 3000:1 900:1 1.8 cm 532 nm 50 cm 633 nm 780 nm 780 nm 11 cm 5.0 cm 2 cm Smallest sample that can be analyzed (independent of stage) Class 1 standard. Class IIIb when fiber optic interface installed and on some specialized accessories. interface installed and on Class IIIb when fiber optic Class 1 standard. Facilitates laser-to-laser and system-to-system reproducibility. and Facilitates laser-to-laser system alignment and calibration upon installation place and automatically optimize Through single USB 2.0 connector. Some accessories may require additional USB or serial ports. Some Through single USB 2.0 connector. 7 2 2 2 1 ) ) ) )) cm 3500 ) cm 1800 cm 6000 -1 -1 -1 -1 -1 -1 5 4 Spectral Range and Resolution Upper cutoff (cm Lower cutoff (cm Upper cutoff (cm Lower cutoff (cm Upper cutoff (cm Lower cutoff (cm Laser safety Laser Power Regulatorabsolute laser power delivered to the sample. Active feedback system to control SmartLock components lock into components (lasers, filters, gratings, fiber port) system Pre-aligned, user-exchangeable Multiple excitation lasersMultiple excitation 532 nm wavelengths 780 nm, 633 nm, Supported 6 Accuracy is based on measurement of polystyrene using peaks at 1001.4, 1602.3, and 3054.3 cm 15-sec measurement time, 5 mW laser power at sample, 25 µm slit aperture. 6. Wavenumber accuracy is measured with full-range grating and without changing lasers or gratings. The spatial resolution achieved also depends on the resolution of the motorized stage. The DXR Raman microscope achieves these specifications with a 100X objective and with all three standard lasers. The difference between system spectral resolution and spectrograph resolution is primarily determined by the excitation laser b 3. 780 nm high-power laser recommended for fiber optic measurements only. Not recommended for microscope work. 5. Average signal-to-noise ratio (peak height/RMS noise) measured using standard polystyrene, full-range grating. 2. 50% maximum transmitted power. The use of some accessories such as fiber optic probes may reduce the spectral range. Check ac 7. Over 10 numbers of exchanges, without recalibration or realignment. Wavenumber accuracy Wavenumber Reproducibility after changing excitation lasers 4. Spatial resolution depends on the wavelength of the excitation laser and the objective used. Spatial Resolution (x, y axes) Sensitivity (signal to noise ratio) 1. The system spectral resolution is measured using ASTM Method E 2529 – 06 and a 100X objective. Additional Performance Specifications High-Resolution Grating Spectral resolution Extended-Range Grating Spectral resolution System Performance – Full-Range Grating Spectral resolution Replaceable Components Smart components Computer Interface Fiber Optic Port to OMNIC Components (lasers, filters, gratings, fiber port) store and report (gratings only) lifetime usage (lasers only) and parameters for calibration component identity, user-installable Optional and General System Features General System Lasers path Single laser beam

DXR Raman Microscope Lasers Gratings General Multiple lasers Optional General Installation Fixed position, pre-aligned, Laser exchange Lasers are pre-aligned and user- user exchangeable without the need exchangeable, no tools required. for tools Laser may be exchanged and shared SmartLock installation Grating is precision-locked into place between any DXR Raman instruments. Smart technology Grating stores serial number, System alignment Automatically optimized upon wavelength, spectral resolution exchange and calibration parameters Smart lasers Laser stores wavelength, serial Grating options Full-range grating and high-resolution number, lifetime usage gratings available for each standard SmartLock installation Precision-locked into place excitation wavelength Laser power regulator Power regulated in 0.1 mW increments. Power at sample is Filters controlled by Laser Power Regulator General Installation Pre-aligned, user-exchangeable and reported in mW. without the need for tools Filtering All lasers include laser line ﬁlters to SmartLock installation Filter block is precision-locked prevent laser artifacts from showing into place up in measured spectra Smart technology Filters store serial number and Depolarization All lasers are depolarized. wavelength Minimizes orientation dependence in measurements. Rayleigh ﬁlters Stokes only 532 nm Laser Laser type Diode-pumped, solid state (DPSS) Laser output power Maximum power at sample 10 mW Fiber Optic Port Lifetime Warranty for 12 months General Installation Pre-aligned, user-installable/ High brightness Yes removable without the need for tools Center wavelength 532 ± 1 nm SmartLock installation Fiber optic port is precision-locked into place Transverse mode TEM 00 Compatibility Compatible with all three standard 2 Beam quality (M )< 1.3 excitation laser frequencies 633 nm Laser Laser type HeNe gas Accepts probes with standard Laser output power Maximum power at sample 8 mW FC connectors Lifetime Warranty for 12 months Smart technology Fiber optic port stores serial number High brightness Yes and identity Center wavelength 632.8 nm

Transverse mode TEM00 Spectrograph Beam quality (M2)< 1.2 Design Triplet Spectrograph No moving parts 780 nm Laser type Frequency-stabilized single mode Spectral Range Absolute 400–1050 nm (high brightness) diode laser Spectral Full-range grating Average 2 cm-1 per CCD pixel Laser output power Maximum power at sample 24 mW Dispersion element Lifetime Warranty for 12 months High-resolution grating Average 1 cm-1 per CCD pixel High brightness Yes element Center wavelength 780 ± 0.2 nm Aperture Four software-selectable 25 and 50 µm pinhole confocal apertures apertures; 25 and 50 µm slit apertures Transverse mode TEM00 Beam quality (M2)< 1.5 780 nm Laser Laser type Multiple transverse mode, (high power) narrow-spectrum diode Laser output power Maximum power at sample 150 mW High brightness No Lifetime Warranty for 12 months Center wavelength 780 ± 0.5 nm Spectral bandwidth < 0.2 nm Beam quality (M2) N/A

DXR Raman microscope is patented by US Patents 6,661,509 B2, 7,460,229 B2, 7,345,760 B2, 7,471,390 B2, 7,233,870 B1 and 7,605,918 B2. Other patents pending. Product Specifications

Microscope Instrument Alignment and Calibration Microscope Infinity-corrected confocal optics Alignment Entirely software- Patented autoalignment technique Stages Manual stage 2" x 3" travel X and Y dimensions controlled aligns laser, Raman emission and Manual Z focus control visual beam paths to microscope Standard motorized stage 5" x 3" travel X and Y dimensions cross-hairs Step size 1 µm Calibration9 Wavelength Software-controlled calibration Software-controlled Z focus calibration using multiple neon emission lines Joystick controller with focus control knob Laser frequency Software-controlled calibration High-precision motorized stage 4" x 3" travel X and Y dimensions calibration using multiple polystyrene Raman Step size 0.1 µm peaks Software-controlled Z focus Intensity calibration Software-controlled calibration using Joystick controller with focus control knob standardized white light sources Stage well-plate adapter Accepts up to 1,536-well microtiter plates 9. Standards incorporated into patented Alignment/Calibration Tool Illuminator options Brightfield microscope Reflection illumination. illuminator, plus nosepiece Accepts brightfield objectives Optional System Validation Brightfield/Darkfield microscope Reflection illumination. Accepts bright- illuminator, plus nosepiece field/darkfield objectives, plus brightfield- ValPro System Available for the DXR Raman microscope only objectives, with adaptor. Supports Qualification optional transmission illumination DQ documentation for hardware and software Objectives Standard working distance objectives 4X, 10X, 20X, 50X, 100X Comprehensive IQ and OQ procedures in the industry- Long working distance objectives 10X, 20X, 50X, 100X standard format Oil immersion objectives 50X, 100X Full system qualification Macro sampling adapter Includes 4X objective, accepts brightfield objectives OQ tests based on industry-standard methods User-supplied objective Must be compatible with Olympus Serialized and traceable standard BF or BD nosepieces8 Software for performance verification tests Microscopy Options Reflected Light Polarized Light Kit/ Support for user-specific OQ or PQ tests Fixed Analyzer Reflected Light DIC Illumination Kit 8. Note that some objectives may be Raman-active and may contribute artifacts to the sample Raman spectrum. Objectives Instrument Serviceability offered by Thermo Scientific have been tested to ensure there is no significant interference with sample measurement. Replacement lasers User-installable OMNIC Software Suite and User Interface Laser Lifetime Laser monitors number of hours of use Smart Components OMNIC checks for laser, grating, filter Laser Power Regulator Reports when laser power at sample compatibility has fallen below laser specification OMNIC restores alignment and calibra- Additional laser, filter, grating sets User-installable tion settings when lasers exchanged User interface Autoexposure Patented. Optimizes exposure time and number of exposures to deliver spectra Physical Dimensions with user-determined signal-to-noise ratio Spatial Dimensions Width: 97 cm Autofocus Optimizes signal from sample Depth: 69 cm Smart background Patented. Collected when instrument is not in use. Eliminates the need for the Height: 61 cm user to collect backgrounds. Weight: 56.7 kg Automated intensity correction Consistent instrument response with all excitation lasers Laser Power Regulator Absolute excitation laser power at the Other Specifications sample controlled by OMNIC Environmental Minimum temperature: 16 °C Laser power at sample reported in mW Maximum temperature: 27 °C Automatic fluorescence correction Compensates for fluorescence prior to data analysis; available for 532, 633 Humidity range: 20–80% and 780 nm excitation wavelengths Power Requirements: 100–250 VAC, 48–63 Hz, 3.2 A max. Excitation laser spot size at sample Reported by OMNIC Regulatory Approval CE, UL/CSA/ETL, 21CFR1040.10 Specialty Software OMNIC Array Automation Automated data collection and post Warranty Information 12-month warranty standard on the collection data analysis from multi-well plates and similar array formats complete DXR Raman Microscope. Extended warranties are available OMNIC Atlµs™ software Provides software-controlled hyper- spectral mapping and image analysis OMNIC Series Software Supports time-evolved data collection OMNIC Macros\Pro Interface for advanced Visual Basic The DXR Raman microscope is a Class IIIb laser-safe product, unless installed with the Class I Laser Safety Enclosure. Installation of a fiber optic probe launcher and programming fiber probe will convert all microscopes to Class IIIb laser-safe, even with the Laser Safety Enclosure installed. Thermo Electron Scientific Instruments LLC, www.thermoscientific.com Madison, WI USA is ISO Certified. ©2008-2010 Thermo Fisher Scientific Inc. All rights reserved. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. PS51549_E 09/10M

Africa-Other +27 11 570 1840 Europe-Other +43 1 333 50 34 0 Japan +81 45 453 9100 Spain +34 914 845 965 Australia +61 3 9757 4300 Finland/Norway/Sweden Latin America +1 561 688 8700 Switzerland +41 61 716 77 00 Austria +43 1 333 50 34 0 +46 8 556 468 00 Middle East +43 1 333 50 34 0 UK +44 1442 233555 Belgium +32 53 73 42 41 France +33 1 60 92 48 00 Netherlands +31 76 579 55 55 USA +1 800 532 4752 Canada +1 800 530 8447 Germany +49 6103 408 1014 New Zealand +64 9 980 6700 China +86 10 8419 3588 India +91 22 6742 9434 Russia/CIS +43 1 333 50 34 0 Denmark +45 70 23 62 60 Italy +39 02 950 591 South Africa +27 11 570 1840