Materia Medica
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Behavior at Spawning of the Trumpet Sea Urchin Toxopneustes Pileolus
“Uncovering” Behavior at Spawning of the Trumpet Sea Urchin Toxopneustes pileolus Andy Chen1 and Keryea Soong2,* 1No. 79-44, Da-Guan Road, Hengchun, Pingtung 946, Taiwan 2Institute of Marine Biology and Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan (Accepted July 29, 2009) The trumpet sea urchin Toxopneustes pileolus (Lamarck, 1816), distributed in shallow reefs of the Indo-West Pacific, is known to possess distinctive globiferous and venomous pedicellariae. The aboral surface of individuals is usually almost fully covered with fragments of dead coral (Fig. 1a) at Hobihu, southern Taiwan (21°56'57"N, 120°44'53"E). The coral fragments may serve as ballast to stabilize the urchins in moving waters, or as shade in well-lit habitats (James 2000, Dumont et al. 2007). Although spawning of many echinoids was reported (Pearse and Cameraon 1991), no information is available for this species or genus. The species was first seen spawning in nature (Fig. 1b) at low tide of a spring tide (1 d after the new moon) on the afternoon of 18 May 2007. In total, 12 individuals were seen to be “naked”, i.e., their aboral surface was almost devoid of coral fragments, and were moving around and waving their tube feet while releasing gametes. The 2nd spawning event was observed under almost the same conditions, i.e., an afternoon low tide of a spring tide (2 d after the new moon) in spring, but 2 yr later, on 26 May 200. Spawning individuals shed the coral fragments before spawning, while non-spawning ones remained covered. -
Tool Use by Four Species of Indo-Pacific Sea Urchins Glyn Barrett1,2, Dominic Revell1, Lucy Harding1, Ian Mills1, Axelle Jorcin1, Klaus M
bioRxiv preprint doi: https://doi.org/10.1101/347914; this version posted June 15, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. Tool use by four species of Indo-Pacific sea urchins Glyn Barrett1,2, Dominic Revell1, Lucy Harding1, Ian Mills1, Axelle Jorcin1, Klaus M. Stiefel1,3,4* 1. People and the Sea, Malapascua Island, Daanbantayan, Cebu, Philippines 2. School of Biological Sciences, University of Reading, UK. 3. Neurolinx Research Institute, La Jolla, CA, USA 4. Marine Science Institute, University of the Philippines, Dilliman, Quezon City, Philippines. *Corresponding author, [email protected] Abstract We compared the covering behavior of four sea urchin species, Tripneustes gratilla, Pseudoboletia maculata, Toxopneutes pileolus, and Salmacis sphaeroides found in the waters of Malapascua Island, Cebu Province and Bolinao, Panagsinan Province, Philippines. Specifically, we measured the amount and type of covering material on each urchin, and, in several cases, the recovery of debris cover after stripping the animal of its cover. We found that Tripneustes gratilla and Salmacis sphaeroides have a higher preference for plant material, especially sea-grass, compared to Pseudoboletia maculata and Toxopneutes pileolus, which prefer to cover themselves with coral rubble and other calcified material. Only for Toxopneutes pileolus did we find a decrease in cover with depth, confirming previous work that the covering behavior serves UV protection. We found no dependence of particle size on either species or urchin size, but we observed that larger urchins carried more and heavier debris. -
The Shallow-Water Macro Echinoderm Fauna of Nha Trang Bay (Vietnam): Status at the Onset of Protection of Habitats
The Shallow-water Macro Echinoderm Fauna of Nha Trang Bay (Vietnam): Status at the Onset of Protection of Habitats Master Thesis in Marine Biology for the degree Candidatus scientiarum Øyvind Fjukmoen Institute of Biology University of Bergen Spring 2006 ABSTRACT Hon Mun Marine Protected Area, in Nha Trang Bay (South Central Vietnam) was established in 2002. In the first period after protection had been initiated, a baseline survey on the shallow-water macro echinoderm fauna was conducted. Reefs in the bay were surveyed by transects and free-swimming observations, over an area of about 6450 m2. The main area focused on was the core zone of the marine reserve, where fishing and harvesting is prohibited. Abundances, body sizes, microhabitat preferences and spatial patterns in distribution for the different species were analysed. A total of 32 different macro echinoderm taxa was recorded (7 crinoids, 9 asteroids, 7 echinoids and 8 holothurians). Reefs surveyed were dominated by the locally very abundant and widely distributed sea urchin Diadema setosum (Leske), which comprised 74% of all specimens counted. Most species were low in numbers, and showed high degree of small- scale spatial variation. Commercially valuable species of sea cucumbers and sea urchins were nearly absent from the reefs. Species inventories of shallow-water asteroids and echinoids in the South China Sea were analysed. The results indicate that the waters of Nha Trang have echinoid and asteroid fauna quite similar to that of the Spratly archipelago. Comparable pristine areas can thus be expected to be found around the offshore islands in the open parts of the South China Sea. -
A Note on the Obligate Symbiotic Association Between Crab Zebrida
Journal of Threatened Taxa | www.threatenedtaxa.org | 26 August 2015 | 7(10): 7726–7728 Note The Toxopneustes pileolus A note on the obligate symbiotic (Image 1) is one of the most association between crab Zebrida adamsii venomous sea urchins. Venom White, 1847 (Decapoda: Pilumnidae) ISSN 0974-7907 (Online) comes from the disc-shaped and Flower Urchin Toxopneustes ISSN 0974-7893 (Print) pedicellariae, which is pale-pink pileolus (Lamarck, 1816) (Camarodonta: with a white rim, but not from the OPEN ACCESS white tip spines. Contact of the Toxopneustidae) from the Gulf of pedicellarae with the human body Mannar, India can lead to numbness and even respiratory difficulties. R. Saravanan 1, N. Ramamoorthy 2, I. Syed Sadiq 3, This species of sea urchin comes under the family K. Shanmuganathan 4 & G. Gopakumar 5 Taxopneustidae which includes 11 other genera and 38 species. The general distribution of the flower urchin 1,2,3,4,5 Marine Biodiversity Division, Mandapam Regional Centre of is Indo-Pacific in a depth range of 0–90 m (Suzuki & Central Marine Fisheries Research Institute (CMFRI), Mandapam Takeda 1974). The genus Toxopneustes has four species Fisheries, Tamil Nadu 623520, India 1 [email protected] (corresponding author), viz., T. elegans Döderlein, 1885, T. maculatus (Lamarck, 2 [email protected], 3 [email protected], 1816), T. pileolus (Lamarck, 1816), T. roseus (A. Agassiz, 5 [email protected] 1863). James (1982, 1983, 1986, 1988, 1989, 2010) and Venkataraman et al. (2013) reported the occurrence of Members of five genera of eumedonid crabs T. pileolus from the Andamans and the Gulf of Mannar, (Echinoecus, Eumedonus, Gonatonotus, Zebridonus and but did not mention the association of Zebrida adamsii Zebrida) are known obligate symbionts on sea urchins with this species. -
Genomics of the Globally Distributed Echinoid Genus Tripneustes
GENOMICS OF THE GLOBALLY DISTRIBUTED ECHINOID GENUS Tripneustes A DISSERTATION SUBMITTED TO THE GRADUATE DIVISION OF THE UNIVERSITY OF HAWAI‘I AT MANOA¯ IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY IN ZOOLOGY (ECOLOGY,EVOLUTION,&CONSERVATION BIOLOGY) May 2018 BY Áki Jarl LÁRUSON DISSERTATION COMMITTEE: Floyd A. Reed, Chairperson Robert C. Thomson Robert J. Toonen Daniel Rubinoff David B. Carlon Keywords: Marine Invertebrate, Phylogenomics, Transcriptomics, Population Genomics © Copyright 2018 – Áki Jarl Láruson All rights reserved i DEDICATION I dedicate this dissertation to my grandfather, Marteinn Jónsson (née Donald L. Martin). ii Acknowledgements Every step towards the completion of this dissertation has been made possible by more people than I could possibly recount. I am profoundly grateful to my teachers, in all their forms, and especially my undergraduate advisor, Dr. Sean Craig, of Humboldt State Uni- versity, for all the opportunities he afforded me in experiencing biological research. My dissertation committee deserves special mention, for perpetually affording me pressing encouragement, but also providing an attitude of support and positivity that has been formative beyond measure. My mentor and committee chair, Dr. Floyd Reed, has pro- vided me with perspectives, insights, and advise that I will carry with me for the rest of my life. My family, although far from the tropical shores of Hawai‘i, have been with me in so many ways throughout this endeavor, and I am so profoundly grateful for their love and support. iii Abstract Understanding genomic divergence can be a key to understanding population dynam- ics. As global climate change continues it becomes especially important to understand how and why populations form and dissipate, and how they may be better protected. -
Reef Monitoring Training
Community Coral Reef Monitoring Training Location: Adelup Point Marybelle Quinata Community Monitoring Coordinator NOAA NOAA Hafa Adai, my name is Agenda • Marine Preserves • Coral Reefs & Their Threats • Overview of Piti-Asan watershed • Ridge-to-Reef Conservation • Survey Methods • Monitoring Exercises • In-Water Training §63116.1. Purpose of Marine Preserves The purpose of the marine preserve is to protect, preserve, manage, and conserve aquatic life, habitat, and marine communities and ecosystems, and to ensure the health, welfare and integrity of marine resources for current and future generations by managing, regulating, restricting, or prohibiting activities to include, but not limited to, fishing, development, human uses.” History of MPAs 1986 1993 1997 Decline in 1st hearing of 3 Legislation passed on Fisheries 5 permanent preserves 1990 1995 2001 Proposal for Marine Full Enforcement 2nd hearing of 3 Preserves Begins NOAA Habitats • Reef Flat • Seagrass • Mixed Coral Stands • Staghorn Thickets • Soft Coral • Sand • Coral Rubble • Pavement/Algae • Reef Margin • Coral • Channels • Fore Reef • Coral • Pavement/Algae • Sand • Channels Burdick 2006 CORALS! What are corals? How do they survive and grow? Why are coral reefs important? Individual Corals Coral Polyp Colonies People Can Disturb The Balance… Fish, Recreational Invertebrates, Overfishing Impacts Turtles, etc. Corals Algae Lack of Public Coral Land-based Bleaching Sources of Awareness & Disease Pollution ©guamreeflife.com ©guamreeflife.com ©guamreeflife.com Burdick et al. -
Equinatoxins: a Review
Toxinology DOI 10.1007/978-94-007-6650-1_1-1 # Springer Science+Business Media Dordrecht 2014 Equinatoxins: A Review Dušan Šuput* Faculty of Medicine, Institute of Pathophysiology and Centre for Clinical Physiology, University of Ljubljana, Ljubljana, Slovenia Abstract Equinatoxins are basic pore forming proteins isolated from the sea anemone Actinia equina. Pore formation is the underlying mechanism of their hemolytic and cytolytic effect. Equinatoxin con- centrations required for pore formation are higher than those causing significant effects in heart and skeletal muscle. This means that other mechanisms must also be involved in the toxic and lethal effects of equinatoxins. Effects of equinatoxins have been studied on lipid bilayers, several cells and cell lines, on isolated organs and in vivo. Different cells have distinct susceptibilities to the toxin, ranging from <1pMupto>100 nM. The cells are swollen after a prolonged treatment with low concentrations of equinatoxin II, or rapidly when 100 nM or higher concentrations of the toxin are used. Equinatoxins increase cation-specific membrane conductance and leakage current, affect the function of potassium and sodium channels in nerve, muscle and erythrocytes, increase intracellular Ca2+ activity, and cause a significant increase of cell volume. In smooth muscle cells and in neuroblastoma NG108-15 cells, an increase in intracellular Ca2+ activity is observed after exposure to 100 nM equinatoxin II. The large difference in toxin concentrations needed for the pore formation and other effects suggest that equinatoxins exert their effects through at least two different mech- anisms. It is well known that lipid environment is important for the proper functioning of membrane channels and other membrane proteins. -
Jo Evans Sea Remedies Leseprobe Sea Remedies Von Jo Evans Herausgeber: Emryss Publisher
Jo Evans Sea Remedies Leseprobe Sea Remedies von Jo Evans Herausgeber: Emryss Publisher http://www.narayana-verlag.de/b7346 Im Narayana Webshop finden Sie alle deutschen und englischen Bücher zu Homöopathie, Alternativmedizin und gesunder Lebensweise. Das Kopieren der Leseproben ist nicht gestattet. Narayana Verlag GmbH, Blumenplatz 2, D-79400 Kandern Tel. +49 7626 9749 700 Email [email protected] http://www.narayana-verlag.de "This book offers an invitation: a subtle and simply irresistible one. From myth to neuroscience, from provings to cases, it offers the reader every possible approach. Diving for our antecedents in evolution, we rediscover senses that we have long forgotten. Emerging from the depths of this work, sea remedies cease to be just remedies. Future homeopathic books will be measured against this one." Franz Swoboda, MD, Austria. Editor of Documenta Homoeopathica, Homoeopathic Physician "A beautifully illustrated and meticulously researched book on the sea remedies that stands to become a classic. Sea Remedies, Evolution of the Senses expands one's understanding of these valuable remedies by providing a vast amount of empirical data on provings and medical application, as well as stimulating the imagination through an intimate understanding of the substance and its role in mythology." Jane Cicchetti, RSHom(NA), CCH, Homeopath,international teacher and author of Dreams, Symbols and Homeopathy, publ. North Atlantic books, USA "This book is the most extensive collection of remedies from the realm of the oceans, and takes homeopathic materia medica to a deeper level of understanding. The author's sensitivity of perception and ability to extract the vital information from natural science, qualitative science, literature and the homeo- pathic knowledge-base, comes together in a coherent presentation of sensations and functions, valuable polarities, and common themes of groups and sub- groups. -
Tool Use by Four Species of Indo-Pacific Sea Urchins
Journal of Marine Science and Engineering Article Tool Use by Four Species of Indo-Pacific Sea Urchins Glyn A. Barrett 1,2,* , Dominic Revell 2, Lucy Harding 2, Ian Mills 2, Axelle Jorcin 2 and Klaus M. Stiefel 2,3,4 1 School of Biological Sciences, University of Reading, Reading RG6 6UR, UK 2 People and The Sea, Logon, Daanbantayan, Cebu 6000, Philippines; [email protected] (D.R.); lucy@peopleandthesea (L.H.); [email protected] (I.M.); [email protected] (A.J.); [email protected] (K.M.S.) 3 Neurolinx Research Institute, La Jolla, CA 92039, USA 4 Marine Science Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines * Correspondence: [email protected] Received: 5 February 2019; Accepted: 14 March 2019; Published: 18 March 2019 Abstract: We compared the covering behavior of four sea urchin species, Tripneustes gratilla, Pseudoboletia maculata, Toxopneustes pileolus, and Salmacis sphaeroides found in the waters of Malapascua Island, Cebu Province and Bolinao, Panagsinan Province, Philippines. Specifically, we measured the amount and type of covering material on each sea urchin, and in several cases, the recovery of debris material after stripping the animal of its cover. We found that Tripneustes gratilla and Salmacis sphaeroides have a higher affinity for plant material, especially seagrass, compared to Pseudoboletia maculata and Toxopneustes pileolus, which prefer to cover themselves with coral rubble and other calcified material. Only in Toxopneustes pileolus did we find a significant corresponding depth-dependent decrease in total cover area, confirming previous work that covering behavior serves as a protection mechanism against UV radiation. We found no dependence of particle size on either species or size of sea urchin, but we observed that larger sea urchins generally carried more and heavier debris. -
Marine Toxins: an Overview
Marine Toxins: An Overview Nobuhiro Fusetani and William Kem 1 Introduction . 2 2 Cyanobacteria . 3 2.1 Microcystins . 3 2.2 Antilatoxin and Kalkitoxin . 4 2.3 Alkaloids. 5 2.4 Polyketides . 6 3 Dinofl agellate (Pyrrophyta) Toxins . 7 3.1 Saxitoxins . 7 3.2 Polyethers . 8 3.3 Long-Chain Polyketides . 11 3.4 Macrolides . 12 4 Macroalgal Toxins . 12 4.1 Kainic and Domoic Acids. 13 4.2 Polycavernosides . 13 4.3 Other Macroalgal Toxins . 13 5 Sponge Toxins . 14 5.1 Polyalkylpyridiniums . 14 5.2 Kainate Receptor Agonists . 15 5.3 Other Sponge Toxins . 15 6 Cnidarian (Coelenterate) Toxins . 15 6.1 Palytoxins . 16 6.2 Cnidarian Peptides . 17 6.3 Cnidarian Protein Toxins . 20 7 Worm Toxins . 22 7.1 Annelid Alkaloids. 22 7.2 Annelid Peptides and Proteins . 22 7.3 Nemertine Alkaloids. 23 7.4 Nemertine Peptide Toxins. 24 7.5 Other Worm Toxins . 26 N. Fusetani () Graduate School of Fisheries Sciences, Hokkaido University, Minato-cho, Hakodate 041-8611, Japan e-mail: [email protected] W. Kem Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL 32610-0267, USA e-mail: [email protected] N. Fusetani and W. Kem (eds.), Marine Toxins as Research Tools, 1 Progress in Molecular and Subcellular Biology, Marine Molecular Biotechnology 46, DOI: 10.1007/978-3-540-87895-7, © Springer-Verlag Berlin Heidelberg 2009 2 N. Fusetani and W. Kem 8 Mollusks. 26 8.1 Paralytic Shellfi sh Poison (PSP) . 26 8.2 Diarrhetic Shellfi sh Poison (DSP) . 26 8.3 Azaspiracid Shellfi sh Poison (AZP) . 28 8.4 Neurotoxic Shellfi sh Poison (NSP). -
Partial Purification and Characterization of a Toxic Substance from Pedicellariae of the Sea Urchin Toxopneustes Pileolus
PARTIAL PURIFICATION AND CHARACTERIZATION OF A TOXIC SUBSTANCE FROM PEDICELLARIAE OF THE SEA URCHIN TOXOPNEUSTES PILEOLUS Hideyuki NAKAGAWA and Akira KIM U RA Departmentof HealthScience, Facultyof Education, Universityof Tokushima,Tokushima 770, Japan Accepted June 26, 1982 A substance in the pedicellariae of the sea 7.1). The mast cells were obtained from the urchin Toxopneustes pileolus induces severe fluid by centrifugation and counted by breath shortening and dizziness in man (1). toluidine blue staining (5) and then resus Kimura et al. (2) partially purified the toxic pended in the buffer at approximately 106 substances from the pedicellariae of T.pileolus cells/ml. The reaction mixture was pre and studied their pharmacological actions. incubated at 37°C in a metabolic shaker for Recently, we found that the ammonium 5 min and the incubation was continued for sulfate fraction of the crude extract from the 10 min with or without the test compound in pedicellariae of T. pileolus caused a release of 2 ml final volume. The reaction was stopped histamine from isolated smooth muscle (3). by chilling the tubes in an ice-bath. The cells Therefore, in this study we have further were then removed from the medium by attempted to purify the ammonium sulfate centrifugation, and 0.5 ml of 2 N perchloric fraction with gel chromatography and acid was added. Histamine was measured characterized the toxic substance. fluorometrically by the method of Shore The crude extract from the pedicellariae of et al. (6). T. pileo/us was partially purified by am Two protein peaks were obtained at the monium sulfate precipitation (30-65% void volume (120 ml, measured by blue saturation) as previously described (3). -
Aquatic Antagonists: Sea Urchin Dermatitis
CLOSE ENCOUNTERS WITH THE ENVIRONMENT Aquatic Antagonists: Sea Urchin Dermatitis Richard Burroughs, MD; Lenard Kerr, DO; Brian Zimmerman, PA; Dirk M. Elston, MD ea urchins can be found in all oceans, and the urchin has no eyes or brain and only a few sensory urchins usually cause human injuries on the organs. Its body is round with a radially symmetri- S foot when stepped on. There are approxi- cal calcite skeleton that is usually made up of mately 800 different species of sea urchins, and tightly interlocking plates that form a rigid struc- most of them are capable of causing human disease ture called the test. The test is covered with a thin when spines are broken off on contact. Some ciliated epithelium. urchins also have stinging pedicellariae containing Urchins have multiple spines that connect at neurotoxins. Injuries from sea urchins often are ball-and-socket–like joints. Muscle fibers wrap misdiagnosed because most clinicians lack familiar- around each base to facilitate movement when the ity with the common clinical, radiological, and his- associated nerve ring is stimulated. Spines are nor- tologic findings.1 For example, sea urchin mally solid and blunt but may be hollow and con- granulomas may mimic infectious granulomas; pen- tain toxins in some species. Spines may be sharp etration injury may result in chronic joint symp- enough to penetrate neoprene suits and gloves.2 toms that may be misdiagnosed as arthritis; and Pedicellariae are small fanglike organs used to neurologic symptoms may be attributed to another inject venom.2,3 The pedicellariae are scattered marine envenomation because the stinging pedi- among the spines and deliver venom even after cellariae of sea urchins do not leave the telltale separation from the sea urchin.