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Prunus Cerasus 1 CPVO-TP/230/1 Date: 15/11/2006 European Union Community Plant Variety Office PROTOCOL FOR DISTINCTNESS, UNIFORMITY AND STABILITY TESTS Prunus cerasus L. and Prunus x gondouinii (Poit. & Turpin) Rehder SOUR CHERRY and DUKE CHERRY UPOV Species Code: PRUNU_CSS, PRUNU_GON Adopted on 15/11/2006 CPVO-TP/230/1 Date: 15/11/2006 I SUBJECT OF THE PROTOCOL The protocol describes the technical procedures to be followed in order to meet the Council Regulation 2100/94 on Community Plant Variety Rights. The technical procedures have been agreed by the Administrative Council and are based on general UPOV Document TG/1/3 and UPOV Guideline TG/230/1 dated 05/04/2006 for the conduct of tests for Distinctness, Uniformity and Stability. This protocol applies for all varieties of Prunus cerasus L. and Prunus x gondouinii (Poit. & Turpin) Rehder . II SUBMISSION OF SEED AND OTHER PLANT MATERIAL 1. The Community Plant Variety Office (CPVO) is responsible for informing the applicant of • the closing date for the receipt of plant material; • the minimum amount and quality of plant material required; • the examination office to which material is to be sent. A sub-sample of the material submitted for test will be held in the variety collection as the definitive sample of the candidate variety. The applicant is responsible for ensuring compliance with any customs and plant health requirements. 2. Final dates for receipt of documentation and material by the Examination Office The final dates for receipt of requests, technical questionnaires and the final date or submission period for plant material will be decided by the CPVO and each Examination Office chosen. The Examination Office is responsible for immediately acknowledging the receipt of requests for testing, and technical questionnaires. Immediately after the closing date for the receipt of plant material the Examination Office should inform the CPVO whether acceptable plant material has been received or not. However if unsatisfactory plant material is submitted the CPVO should be informed as soon as possible. 3. Plant material requirements The current quality and quantity requirements as well as the final dates for submission of the plant material are available on the CPVO website (www.cpvo.europa.eu ) and are published in the CPVO gazette ‘S2’. 2 CPVO-TP/230/1 Final English Date: 15/11/2006 Quality of plants: Should not be less than the standards laid down in Council 2000/29/EC and its amendments concerning quarantine organisms, and Council Directive 92/34/EEC and Commission Directive 93/48/EEC and their amendments concerning organisms impairing quality, at the date of adoption of this protocol; please refer to “Eur-Lex” for the full text and in case of any subsequent amendments to the four aforesaid Directives . Quarantine organism (Directive 2000/29/EC). The plant material must be free from: Insects, mites and nematodes at all stages of their development - Acleris spp. (non-European) - Amauromyza maculosa - Anomala orientalis - Anoplophora chinensis - Anoplophora malasiaca - Arrhenodes minutus - Bemisia tabaci (non-European populations) - Carposina niponensis - Choristoneura spp. (non-European) - Cicadellidae (non-European) - Conotrachelus nenuphar - Enarmonia prunivora - Enarmonia packardi - Globodera pallida - Globodera rostochiensis - Grapholita inopinata - Heliothis armigera - Heliothis zea - Liriomyza bryoniae - Liriomyza huidobrensis - Liriomyza trifolii - Liriomyza sativae - Longidorus diadecturus - Monochamus spp. (non-European) - Myndus crudus - Nacobbus aberrans - Opogona sacchari - Popilia japonica - Premnotrypes spp. (non-European) - Pseudopityophthorus minutissimus - Pseudopityophthorus pruinosus - Scaphoideus luteolus - Spodoptera eridania - Spodoptera frugiperda - Spodoptera littoralis - Spodoptera litura 3 CPVO-TP/230/1 Final English Date: 15/11/2006 - Tephritidae (non-European) - Trachypterellus quadrigibbus - Thrips palmi - Xiphinema americanum (non-European populations) - Xiphinema californicum Bacteria - Clavibacter michiganensis - Erwinia amylovora - Pseudonomas solanacearum - Xylella fastidiosa Fungi - Alternaria alternata - Ceratocystis fagacearum - Chrysomixa arctostaphyli - Cronartium spp. (non-European) - Endocronartium spp. (non-European) - Guignardia laricina - Guignardia piricola - Gymnosporangium spp. (non-European) - Inonotus weirii - Melampsora medusae - Melampsora farlowii - Monilinia fructicola - Mycosphaerella larici-leptolepis - Mycosphaerella populorum - Phoma andina - Phyllosticta solitaria - Septoria lycopersici - Synchytrium endobioticum - Thecaphora solani - Trechispora brinkmannii Viruses and virus-like organisms 1. Elm phlöem mycoplasm 2. Potato viruses and virus-like organisms such as: - Andean potato latent virus - Andean potato mottle virus - Arracha virus B, oca strain - Potato black ringspot virus - Potato spindle tuber viroid - Potato virus T - Non-European isolates of potato viruses A, M, S, V, X and Y (including Yo, Yn and Yc) and Potato leafroll virus 3. Tobacco ringspot virus 4. Tomato ringspot virus 4 CPVO-TP/230/1 Final English Date: 15/11/2006 5. Viruses and virus-like organisms of Cydonia Mill., Fragaria L., Malus Mill., Prunus L., Pyrus L., Ribes L., Rubus L., and Vitis L., such as: - Apple proliferation mycoplasm - Apricot chlorotic leafroll mycoplasm - Blueberry leaf mottle virus - Cherry rasp leaf mottle virus (American) - Peach mosaic virus (American) - Peach phony rickettsia - Peach rosette mosaic virus - Peach rosette mycoplasm - Peach-X disease mycoplasm - Peach yellows mycoplasm - Pear decline mycoplasm - Plum line pattern virus (American) - Raspberry leaf curl virus (American) - Strawberry latent “C” virus - Strawberry vein banding virus - Strawberry witches’ broom mycoplasm - Non-European viruses and virus-like organisms of Cydonia Mill., Fragaria L., Malus Mill., Prunus L., Pyrus L., Ribes L., Rubus L., and Vitis L., 6. Viruses transmitted by Bemisia tabaci Genn, such as: - Bean golden mosaic virus - Cowpea mild mottle virus - Lettuce infectious yellows virus - Pepper mild tigré virus - Squash leaf curl virus - Euphorbia mosaic virus - Florida tomato virus Parasitic plants - Arceuthobium spp. (non-European) Organisms impairing quality (Directive 92/34/EEC and 93/48/EEC. The plant material must, at least on visual inspection, be substantially free from any harmful organisms and diseases impairing quality or any signs or symptoms thereof and in particular be free from: Insects, mites and nematodes at all stages of their development - Capnodis tenebrionis - Meloidogyne spp . - Scale insects, in particular: Epidiaspis leperii, Pseudaulacaspis pentagona, Quadraspidiotus perniciosus 5 CPVO-TP/230/1 Final English Date: 15/11/2006 Bacteria - Agrobacterium tumefaciens - Pseudomonas syringae pv . mors prunorum - Pseudomonas syringae pv . syringae Fungi - Armillariella mellea - Chondrostereum purpureum - Nectria galligena - Rosellinia necatrix - Verticillium spp. Viruses and virus-like organisms - Prune dwarf virus - Prunus necrotic ringspot virus Chemical treatment: The plant material must not have undergone any treatment unless the CPVO and the examination office allow or request such treatment. If it has been treated, full details of the treatment must be given. Labelling of individual - Species plants in sample: - File number of the application allocated by the CPVO - Breeder's reference - Examination office’s reference (if known) - Name of applicant - The phrase “On request of the CPVO” III CONDUCT OF TESTS 1. Variety collection A variety collection will be maintained for the purpose of establishing distinctness of the candidate varieties in test. A variety collection may contain both living material and descriptive information. A variety will be included in a variety collection only if plant material is available to make a technical examination. Pursuant to Article 7 of Council Regulation No. 2100/94, the basis for a collection should be the following: • varieties listed or protected at the EU level or at least in one of the EEA Member States; • varieties protected in other UPOV Member States; • any other variety in common knowledge. 6 CPVO-TP/230/1 Final English Date: 15/11/2006 The composition of the variety collection in each Examination Office depends on the environmental conditions in which the Examination Office is located. Variety collections will be held under conditions which ensure the long term maintenance of each accession. It is the responsibility of Examination Offices to replace reference material which has deteriorated or become depleted. Replacement material can only be introduced if appropriate tests confirm conformity with the existing reference material. If any difficulties arise for the replacement of reference material, Examination Offices must inform the CPVO. If authentic plant material of a variety cannot be supplied to an Examination Office the variety will be removed from the variety collection. 2. Material to be examined Candidate varieties will be directly compared with other candidates for Community plant variety rights tested at the same Examination Office, and with appropriate varieties in the variety collection. When necessary an Examination Office may also include other candidates and varieties. Examination Offices should therefore make efforts to co-ordinate the work with other Offices involved in DUS testing of sour cherry. There should be at least an exchange of technical questionnaires for each candidate variety, and during the test period, Examination Offices should notify each other and
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