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Practical Applications of Digital Pathology Molecular Technologies in the Clinical Diagnostic Laboratory Molecular Assays In cancercontroljournal.org Vol. 22, No. 2, April 2015 H. LEE MOFFITT CANCER CENTER & RESEARCH INSTITUTE, AN NCI COMPREHENSIVE CANCER CENTER Practical Applications of Digital Pathology Molecular Technologies in the Clinical Diagnostic Laboratory Molecular Assays in Cytopathology for Thyroid Cancer Biomarkers in Hematological Malignancies: A Review of Molecular Testing in Hematopathology Circulating Tumor Cells: A Window Into Tumor Development and Therapeutic Effectiveness Surgical and Molecular Pathology of Barrett Esophagus Molecular Pathology of Soft-Tissue Neoplasms and Its Role in Clinical Practice Advances in EGFR as a Predictive Marker in Lung Adenocarcinoma Impending Impact of Molecular Pathology on Classifying Adult Diffuse Gliomas Overexpression of Vascular Endothelial Growth Factor A in Invasive Micropapillary Colorectal Carcinoma Advances in the Molecular Analysis of Breast Cancer: Pathway Toward Personalized Medicine Use of Immunohistochemical Stains in Epithelial Lesions of the Breast Special Technologies for Ex Vivo Analysis of Cancer Cancer Control is included in Index Medicus/MEDLINE Permit No. 4390 No. Permit Jacksonville, FL Jacksonville, Tampa FL 33612-9416 FL Tampa PAID 12902 Magnolia Drive Magnolia 12902 & RESEARCH INSTITUTE INC INSTITUTE RESEARCH & Nonprofit Org. Nonprofit CENTER CANCER MOFFITT LEE H MOFFITT PERFORMS THE MOST BLOOD & MARROW TRANSPLANTS IN FLORIDA TOGETHER WE CAN OFFER YOUR PATIENTS: • Transplant as an option. With lower treatment toxicities and better donor matching, candidacy is no longer limited solely by chronological age • Expertise in donor selection and state-of-the-art transplant strategies • The ability to have post-transplant care, including immunosuppression suppression, managed close to home. Our commitment to you is close communication regarding our shared patients • Transplant at the optimal time. Timing of referral for transplant may impact outcomes TO REFER A PATIENT OR DISCUSS OPTIMAL REFERRAL TIMING, SPEAK TO ONE OF OUR TRANSPLANT PHYSICIANS. CALL 1-888-MOFFITT MOFFITT PERFORMS THE Editorial Board Members Editor: Rami Komrokji, MD Production Staff: Associate Member Lodovico Balducci, MD Veronica Nemeth Malignant Hematology MOST BLOOD & MARROW Senior Member Editorial Coordinator Conor C. Lynch, PhD Program Leader, Senior Adult Oncology Program Don Buchanan Assistant Member Moffitt Cancer Center Graphic Designer TRANSPLANTS IN FLORIDA Tumor Biology Deputy Editor: Amit Mahipal, MD Consultants: Assistant Member Julio M. Pow-Sang, MD Sherri Damlo Senior Member Clinical Research Unit Gastrointestinal Oncology Medical Copy Editor Chair, Department of Genitourinary Oncology and Art Coordinator Director of Moffitt Robotics Program Kristen J. Otto, MD Kelly Young Moffitt Cancer Center Assistant Member Graphic Designer Head & Neck Oncology Editor Emeritus: Michael A. Poch, MD Associate Editor of Assistant Member John Horton, MB, ChB Educational Projects Professor Emeritus of Medicine & Oncology Genitourinary Oncology John M. York, PharmD Jeffery S. Russell, MD, PhD Akita Biomedical Consulting Moffitt Cancer Center Assistant Member 1111 Bailey Drive Endocrine Tumor Oncology Journal Advisory Committee: Paso Robles, CA 93446 Aliyah Baluch, MD Elizabeth M. Sagatys, MD Phone: 805-238-2485 Assistant Member Assistant Member Fax: 949-203-6115 Infectious Diseases Pathology - Clinical E-mail: [email protected] Dung-Tsa Chen, PhD Jose E. Sarria, MD Associate Member Assistant Member For Consumer and Biostatistics Anesthesiology General Advertising Hey Sook Chon, MD Saïd M. Sebti, PhD Information: Assistant Member Senior Member Veronica Nemeth Gynecological Oncology Drug Discovery Editorial Coordinator Jasreman Dhillon, MD Bijal D. Shah, MD Cancer Control: Assistant Member Assistant Member Journal of the Moffitt Cancer Center Pathology - Anatomic Malignant Hematology 12902 Magnolia Drive – MBC-JRNL Tampa, FL 33612 Jennifer S. Drukteinis, MD Lubomir Sokol, MD, PhD Phone: 813-745-1348 Associate Member Senior Member Fax: 813-449-8680 Diagnostic Radiology Hematology/Oncology E-mail: [email protected] Timothy J. George, PharmD Hatem H. Soliman, MD Pharmacy Residency Director Assistant Member Clinical Pharmacist - Malignant Hematology Breast Oncology Clement K. Gwede, PhD Jonathan R. Strosberg, MD Associate Member Assistant Member Health Outcomes & Behavior Gastrointestinal Oncology Sarah E. Hoffe, MD Sarah W. Thirlwell, RN Associate Member Nurse Director Radiation Oncology Supportive Care Medicine Program Cancer Control is a member of John V. Kiluk, MD Eric M. Toloza, MD, PhD the Medscape Publishers’ Circle®, Associate Member Assistant Member an alliance of leading medical Breast Oncology Thoracic Oncology publishers whose content is Richard D. Kim, MD Nam D. Tran, MD featured on Medscape Associate Member Assistant Member (www.medscape.com). Gastrointestinal Oncology Neuro-Oncology Bela Kis, MD, PhD Jonathan S. Zager, MD Most issues and supplements of Assistant Member Associate Member Cancer Control are available at Diagnostic Radiology Sarcoma and Cutaneous Oncology cancercontroljournal.org CANCER CONTROL: JOURNAL OF THE MOFFITT CANCER CENTER (ISSN 1073-2748) is published by H. Lee Moffitt Cancer Center & Research Institute, 12902 Magnolia Drive, Tampa, FL 33612. Telephone: 813-745-1348. Fax: 813-449-8680. E-mail: [email protected]. Internet address: cancercontroljournal.org. Cancer Control is included in Index Medicus ®/MEDLINE® and EMBASE®/ Excerpta Medica, Thomson Reuters Science Citation Index Expanded (SciSearch®) and Journal Citation Reports/Science Edition. Copyright 2015 by H. Lee Moffitt Cancer Center & Research Institute. All rights reserved. Cancer Control: Journal of the Moffitt Cancer Center is a peer-reviewed journal that is published to enhance the knowledge needed by professionals in oncology to help them minimize the impact of human malignancy. Each issue emphasizes a specific theme relating to the detection or management of cancer. The objectives of Cancer Control are to define the current state of cancer care, to integrate recently generated information with historical practice patterns, and to enlighten readers through critical reviews, commentaries, and analyses of recent research studies. DISCLAIMER: All articles published in this journal, including editorials and letters, represent the opinions of the author(s) and do not necessarily reflect the opinions of the editorial board, the H. Lee Moffitt Cancer Center & Research Institute, Inc, or the institutions with which the authors are affiliated unless clearly specified. The reader is advised to independently verify the effectiveness of all methods of treatment and the accuracy of all drug names, dosages, and schedules. Dosages and methods of administration of pharmaceutical products may not be those listed in the package insert and solely reflect the experience of the author(s) and/or clinical investigator(s). April 2015, Vol. 22, No. 2 Cancer Control 129 Table of Contents Editorial Personalizing the Attack on Cancer: Zeroing in on Targets 133 Anthony M. Magliocco, MD Articles Practical Applications of Digital Pathology 137 Daryoush Saeed-Vafa, MD, and Anthony M. Magliocco, MD Molecular Technologies in the Clinical Diagnostic Laboratory 142 Victor E. Zota, MD, and Anthony M. Magliocco, MD Molecular Assays in Cytopathology for Thyroid Cancer 152 Pablo Valderrabano, MD, Victor E. Zota, MD, Bryan McIver, MD, PhD, Domenico Coppola, MD, and Marino E. Leon, MD Biomarkers in Hematological Malignancies: 158 A Review of Molecular Testing in Hematopathology Mohammad Hussaini, MD Circulating Tumor Cells: A Window Into Tumor Development 167 and Therapeutic Effectiveness Gisela Cáceres, PhD, John A. Puskas, PhD, and Anthony M. Magliocco, MD Surgical and Molecular Pathology of Barrett Esophagus 177 Sherma Zibadi, MD, PhD, and Domenico Coppola, MD Molecular Pathology of Soft-Tissue Neoplasms and Its Role in Clinical Practice 186 Evita B. Henderson-Jackson, MD, and Marilyn M. Bui, MD, PhD 130 Cancer Control April 2015, Vol. 22, No. 2 Table of Contents Advances in EGFR as a Predictive Marker in Lung Adenocarcinoma 193 Farah K. Khalil, MD, and Soner Altiok, MD, PhD Impending Impact of Molecular Pathology on Classifying Adult Diffuse Gliomas 200 Robert J. Macaulay, MD Overexpression of Vascular Endothelial Growth Factor A in 206 Invasive Micropapillary Colorectal Carcinoma Marilin Rosa, MD, Maisoun Abdelbaqi, MD, Katherine M. Bui, Aejaz Nasir, MD, MPhil, Marilyn M. Bui, MD, PhD, David Shibata, MD, and Domenico Coppola, MD Advances in the Molecular Analysis of Breast Cancer: 211 Pathway Toward Personalized Medicine Marilin Rosa, MD Use of Immunohistochemical Stains in Epithelial Lesions of the Breast 220 Laila Khazai, MD, and Marilin Rosa, MD Special Technologies for Ex Vivo Analysis of Cancer 226 Jenny M. Kreahling, PhD, and Soner Altiok, MD, PhD Departments Editorial: Should We Preclude Phase 1 Clinical Trials From Enrolling Elderly Individuals? 232 Lodovico Balducci, MD Original Research: Effect of Age on Clinical Outcomes in Phase 1 Trial Participants 235 Amit Mahipal, MD, Aaron C. Denson, MD, Benjamin Djulbegovic, MD, PhD, Richard Lush, PhD, Ambuj Kumar, MD, Tzu-Hua Juan, MS, Michael J. Schell, PhD, and Daniel M. Sullivan, MD Case Report: Primary Enteropathy-Associated T-Cell Lymphoma Type 2: An Emerging Entity? 242 Nicole M. Grigg-Gutierrez, MD, Rodolfo Estremera-Marcial, MD, William W. Cáceres, MD, and Doris H. Toro, MD April 2015, Vol. 22, No. 2 Cancer Control 131 Table of Contents Special Report: Cost Effectiveness
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