Prevalence, Intensity and Factors Associated with Soil
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PREVALENCE, INTENSITY AND FACTORS ASSOCIATED WITH SOIL TRANSMITTED HELMINTHS INFECTION AMONG CHILDREN IN CHILILABOMBWE DISTRICT, COPPERBELT PROVINCE, ZAMBIA-CROSS SECTIONAL STUDY By Sibongile Tembo A dissertation submitted in partial fulfilment of the requirements for the award of Master of Science in Epidemiology THE UNIVERSITY OF ZAMBIA LUSAKA April, 2019 COPYRIGHT No part of this study may be reproduced or stored in any form either electronically, mechanically, photocopying, recording or otherwise without prior written permission from the author or the University of Zambia. ii DECLARATION I hereby declare that works presented in this study for the Master of Science in Epidemiology has not been presented whether wholly or in part for any other study programme and is not being submitted for any other Masters programme. The result is entirely the results of my own independent investigation. The various resources to which I am indebted have been acknowledged. Signed: ………………………………………………. Sibongile Tembo (Candidate) iii APPROVAL This dissertation of Sibongile Tembo has been approved as fulfilling the requirements for the award of Master of Science in Epidemiology by the University of Zambia. Examiner 1: Name: ……………………… Signature……………………… Date: …………………… Examiner 2: Name: ……………………… Signature……………………… Date: …………………… . Examiner 3: Name: ……………………… Signature……………………… Date: …………………… Chairperson Board of Examiners: Name: ……………………… Signature……………………… Date: ……………………. Supervisor: Ms. Jessy Zgambo Signature……………………… Date: …………………… iv ABSTRACT Soil transmitted helminths (STH) are among the Neglected Tropical Diseases (NTDs) of Poverty. They are a common type of parasitic infections in the world, caused by three main species commonly known as roundworms, whipworms and hookworms. This study investigated the prevalence, intensity and factors associated with soil transmitted helminths among children in Chililabombwe district of Zambia. The study was conducted between October and December, 2017. A cross sectional design was used, consisting of 411 guardian – child pairs. A questionnaire and checklist was used to collect data on factors associated with helminth infection. Stool samples were collected and examined for presence of parasites using formal-ether concentration and Kato Katz techniques. Geometric mean was used to report intensity of infection. Bivariate and multivariate logistic regression was used to determine factors associated with worm infestations. An investigator led stepwise regression was used to identify factors associated with developing STH infection and level of significance was set at 0.05. Prevalence of soil transmitted helminth infection was 14.4% and the most dominant parasite was round worms (14.1%). The overall intensity of infection was light (<5000epg) with a few heavy infections (>50000epg). Factors independently associated with soil transmitted helminth infection after adjusting for other variables were residence (AOR=0.26; 95% CI [0.09-0.73]), household income (AOR=2.49; 95% CI [1.01-6.12]) and overcrowding (AOR =1.33; 95% CI [1.09-1.62]). This indicates that reinfection is common even after deworming. Hence policy makers should advocate for improved living conditions in urban areas coupled with reduced household overcrowding. Key Words: Soil transmitted helminths, Ascaris lubricoides, Trichuris trichiura, Hookworms, Intensity, Neglected tropical diseases of poverty, Zambia v DEDICATION I dedicate this dissertation to God Almighty for being the source of my strength throughout this programme. Thank you God for your unending love and guidance, may your name be praised. I also dedicate this work to my parents Edward Tembo and Margarete Chola Musebe for their values and morals that they handed down to me during the course of their lives. Continue resting in peace. Not forgetting my siblings and friends for the support during the course of my programme. Thank you! My love for you can never be quantified. God bless you. vi ACKNOWLEDGEMENT This research would not have been possible without the help and support of numerous people. Precisely, I would like to thank my supervisors Ms. Jessy Zgambo, Ms. Lungowe Sitali and Ms. Patricia Mubita who provided valuable guidance and mentorship throughout the course of my research. Special tributes also goes to Mr. Zimba Japhet, Dr Jeremiah Banda, Dr Chongwe, Dr Mweene, Dr Halwindi, Mr. Banda, Mr. Mumbi and the entire school of public health for providing the necessary skills to enable the success of this research project. I also wish to extend my gratitude to Ministry of Higher Education for sponsoring my Master’s Degree Programme and for providing a financial grant to support this research. Furthermore, my sincere gratitude goes to Chililabombwe District Health Office under the leadership of Dr M Chakulimba (District Health Director) for granting permission to conduct research in the district and availing the district laboratory staff, equipment and infrastructure to undertake the study. To Mrs. Mwale, Mr. Sianongo and the team, your efforts and contributions can never go without being recognized. Finally, I would say thank you to my data collectors, family and friends. Prominent among these are Mr. Mabvuto Tembo, Mr. Chella Musonda, and Mr. Francis Katayi Pande for your unwavering support rendered to me, this was emotional, financial and spiritual support. Mr. Bwalya, Mr. Chella Makumba and Mr. Kamayani Mapeesho and the MPH class of 2016 for the support and encouragements. Thank you for making my journey easy. God bless you all. vii TABLE OF CONTENTS COPYRIGHT .............................................................................................................................................. ii APPROVAL ............................................................................................................................................... iv ABSTRACT ................................................................................................................................................. v DEDICATION............................................................................................................................................ vi ACKNOWLEDGEMENT ........................................................................................................................ vii LIST OF APPENDICES ......................................................................................................................... xiii ABBREVIATIONS AND ACRONYMS ................................................................................................ xiv DEFINITION OF TERMS ....................................................................................................................... xv CHAPTER 1: INTRODUCTION .............................................................................................................. 1 1.1 Background ......................................................................................................................................... 1 1.2 Problem Statement .............................................................................................................................. 3 2.3 General Objectivities .......................................................................................................................... 4 2.3.1 Specific Objectives ...................................................................................................................... 4 2.4 Research Questions ............................................................................................................................. 4 1.5 Justification ......................................................................................................................................... 5 1.6 Organization of Dissertation ............................................................................................................... 5 CHAPTER 2: LITERATURE REVIEW .................................................................................................. 7 2.1 Overview ............................................................................................................................................. 7 2.2 Risk Factors associated with Soil Transmitted Helminths .................................................................. 8 2.2.1 Age group and sex........................................................................................................................ 8 2.2.2 Water, Sanitation and Hygiene (WASH) ..................................................................................... 9 2.2.3 Exposed bare feet ....................................................................................................................... 12 viii 2.2.4 Literacy of Parents ..................................................................................................................... 12 2.2.5 Climate factors ........................................................................................................................... 13 2.2.6 Urbanization and Settlement Patterns ........................................................................................ 13 CHAPTER 3: METHODOLOGY ........................................................................................................... 15 3.1 Study Design ..................................................................................................................................... 15 3.2 Study Setting ....................................................................................................................................