Herpesviral Replication Compartments Move and Coalesce at Nuclear
Herpesviral replication compartments move and PNAS PLUS coalesce at nuclear speckles to enhance export of viral late mRNA Lynne Changa, William J. Godinezb, Il-Han Kimb, Marco Tektonidisb, Primal de Lanerollec, Roland Eilsb, Karl Rohrb, and David M. Knipea,1 aDepartment of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115; bDepartment of Bioinformatics and Functional Genomics, Biomedical Computer Vision Group, BIOQUANT, Institute of Pharmacy and Molecular Biotechnology (IPMB), University of Heidelberg and German Cancer Research Center (DKFZ) Heidelberg, 69120 Heidelberg, Germany; and cDepartment of Physiology and Biophysics, College of Medicine, University of Illinois, Chicago, IL 60612 Edited* by John J. Mekalanos, Harvard Medical School, Boston, MA, and approved April 11, 2011 (received for review March 1, 2011) The role of the intranuclear movement of chromatin in gene Viruses are simple genetic entities and have often provided expression is not well-understood. Herpes simplex virus forms precise probes of cellular molecular mechanisms. Viral chro- replication compartments (RCs) in infected cell nuclei as sites of mosomes of herpes simplex virus (HSV) are replicated and un- viral DNA replication and late gene transcription. These structures dergo late transcription in intranuclear structures called replica- develop from small compartments that grow in size, move, and tion compartments (RCs) (15), which are known to move and coalesce. Quantitative analysis of RC trajectories, derived from 4D coalesce in nuclei of infected cells during the course of infection images, shows that most RCs move by directed motion. Directed (16, 17). The function and mechanism of RC movement are movement is impaired in the presence of actin and myosin inhibitors unknown.
[Show full text]