54 Cucumeropsis Mannii Reverses High-Fat Diet Induced Metabolic
[Frontiers in Bioscience, Elite, 13, 54-76, Jan 1, 2021] Cucumeropsis mannii reverses high-fat diet induced metabolic derangement and oxidative stress Anthony T Olofinnade1, Adejoke Y Onaolapo2, Azurra Stefanucci3, Adriano Mollica3, Olugbenga A Olowe4, Olakunle J Onaolapo5 1Department of Pharmacology, Therapeutics and Toxicology, Faculty of Basic Clinical Sciences, College of Medicine, Lagos State University, Ikeja, Lagos State, Nigeria, 2Behavioral Neuroscience and Neurobiology Unit, Department of Anatomy, Ladoke Akintola University of Technology, Ogbomosho, Oyo State, Nigeria, 3Department of Pharmacy, University “G. d’Annunzio” of Chieti-Pescara, Chieti, Italy, 4Molecular Bacteriology Unit, Department of Microbiology and Parasitology, Ladoke Akintola University of Technology, Ogbomosho, Oyo State, Nigeria, 5Behavioral Neuroscience and Neuropharmacology Unit, Department of Pharmacology, Ladoke Akintola University of Technology, Osogbo, Osun State, Nigeria TABLE OF CONTENTS 1. Abstract 2. Introduction 3. Materials and methods 3.1. Chemicals 3.2. Cucumeropsis mannii 3.3. Phytochemical screening 3.3.1. Determination of total phenol contents 3.3.2. Determination of total flavonoid content 3.3.3. Determination of ascorbic acid content 3.3.4. Antioxidant activity 3.4. Animals 3.5. Diet 3.6. Experimental method 3.7. Behavioural tests 3.7.1. Open field 3.7.2. Memory tests 3.7.3. Anxiety-related behaviour in the elevated plus maze 3.8. Blood collection 3.9. Tissue homogenisation 3.10. Biochemical tests 3.10.1. Liver and renal function tests 3.10.2. Lipid profile 3.10.3. Superoxide dismutase 3.10.4. Lipid peroxidation (Malondialdehyde) 3.10.5 Acetylcholinesterase activity 3.10.6. γ-amino-butyric acid (GABA) levels 3.11.
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