DOCSLIB.ORG

A Holobiont Characterization of Reproduction in a Live-Bearing

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
A Holobiont Characterization of Reproduction in a Live-Bearing A holobiont characterization of reproduction in a live-bearing cockroach, Diploptera punctata A dissertation submitted to the Graduate School of the University of Cincinnati in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Biological Sciences of the McMicken College of Arts and Sciences By Emily C. Jennings B.S. Neurobiology, University of Cincinnati, April 2013 Committee Chair: Joshua B. Benoit, Ph.D. Abstract Viviparous reproduction is characterized by maternal retention of developing offspring within the reproductive tract during gestation, culminating in live birth. In some cases, a mother will provide nutrition beyond that present in the yolk; this is known as matrotrophic viviparity. While this phenomenon is best associated with mammals, it is observed in insects such as the viviparous cockroach, Diploptera punctata. Female D. punctata carry developing embryos in the brood sac, a reproductive organ that acts as both a uterus and placenta by protecting and providing a nutritive secretion to the intrauterine developing progeny. While the basic physiology and hormonal changes of D. punctata pregnancy have been characterized, little else is known about this phenomenon. This study attempts to broaden the understanding of D. punctata reproduction by using a multi-omics approach to characterize multiple aspects of the holobiome. First, I utilized RNA-seq analysis to characterize molecular changes associated with D. punctata reproduction and provides the most complete gene set to date for this species. A comparison of four stages of the female reproductive cycle revealed unique gene expression profiles corresponding to each stage. Differentially regulated transcripts of interest include the previously identified family of milk proteins, transcripts associated with juvenile hormone metabolism, and other reproduction-associated transcripts. I next utilized 16S rRNA gene sequencing to characterize the microbiome of Diploptera punctata during development, from embryonic development to female adulthood. We identified 50 phyla and 121 classes overall and found that mothers and their developing embryos had significantly different microbial communities, with embryos harboring only a single endosymbiont known as Blattabacteria. Our analysis of postnatal development reveals i that significant amounts of non-Blattabacteria species are not able to colonize newborn D. punctata until melanization in the first instar, after which the microbial community rapidly and dynamically diversifies. This rapid change of the microbial community appears to stabilize after the second juvenile stage with no major changes even through the last molt to adulthood for females. Lastly, I sought to better understand the role of the cockroach specific endosymbiont Blattabacteria as the sole member of the D. punctata embryonic microbial community. We leveraged next generation genome sequencing to computationally characterize the metabolic capacity of Blattabacteria isolated from D. punctata. Our analysis produced a 670,273 base pair chromosome with a high degree of similarity to previously sequenced genomes of Blattabacteria isolated from other cockroach species. Our genome assembly contained 593 putatively protein coding DNA sequences, which included proteins required for nearly all key metabolic processes including DNA replication, transcription, and translation. Additionally, the D. punctata strain of Blattabacteria also holds the ability to synthesize nearly all 10 essential amino acids and process nitrogenous waste to do so. This suggests that Blattabacteria are likely providing nutritional supplementation to intrauterine developing embryos to accommodate the previously reported lack of methionine and tryptophan in maternally provided nutrition. Together, the research presented in this dissertation contributes to a more holistic understanding of this human-like reproductive mode in the cockroach Diploptera punctata, broadening our understanding of viviparity throughout the animal kingdom. ii © Copyright Emily C Jennings 2019 All Rights Reserved iii Acknowledgements First and foremost, I want to thank my advisor, Dr. Joshua Benoit, for going out on a limb and taking me on as one of two graduate students the same year he started as a new faculty member at UC. He provided constant support and exhibited unfathomable patience as I stumbled through the last six years in his lab. He encouraged me to think not only critically as a scientist but creatively, encouraging me to take risks on wild experiments like modeling fetal alcohol syndrome in cockroaches. When some of those risks resulted in failed experiments Josh was always there to help me bounce back, just as he was always there to casually offer kudos when something did manage to work out. Josh helped keep me on track when I started to lose focus and dealt with my constant questions. He never questioned my decisions to enroll in courses outside of traditional biology curriculum, whether it was a course about birth defects, or an undergraduate level python programming course. When I realized that I was interested in at least trying a career outside of academia after graduating, he never wavered in his support even when I felt like a quitter. Josh was always open to and encouraged discussions about my project, which sometimes turned into fun debates about where the experiment was going or needed to go. What wasn’t always fun was when one of us had to admit we were wrong, but Josh was a good sport and never gloated too much when I was wrong even though I wasn’t always so kind when he was wrong. It’s been a wonderful six years in the lab, and I know I’ll miss it when I’m gone. I’d also like to acknowledge and thank Dr. Elke Buschbeck, Dr. Joshua Gross, Dr. Mike Polak, and Dr. Matthew Weirauch, for being part of this crazy journey as members of my research advisory committee. They have offered invaluable guidance and support, helping me iv to navigate and focus my disjointed project ideas, overcome the roadblocks of failed experiments and helping to know when it was time to let go of a stalled-out project. Through it all, I’ve always been able to count on them to be in my corner of the ring. In addition to serving on my advisory committee, Dr. Elke Buschbeck has played an especially important role in this journey. I’d like to thank her for gambling on hiring me, a weird bug fanatic in her neuroscience course, as a work study back in 2011. She has consistently served as a mentor and role model, helping me grow as a scientist. I cannot begin to describe the value of her constant encouragement and support, letting me know when I was too hard on myself but also making sure to ask if I had something better to do when she noticed I was slacking. I want to extend my gratitude to all of the current and former members of the Benoit lab for their constant support, listening to me whine when I needed to, dealing with me talking to myself at the lab bench or in our shared office space, and keeping things fun in and out of lab. This work would not be possible without the undergraduate students who have been part of our lab throughout the last 6 years. LaVeta Burke, Luke Bernhardt, John Cavanaugh, Jake Hendershot, Matthew Korthauer, Erica McDonald, and Sophie Shemas have all made contributions to my research, running countless experiments. Working with them has taught me so much about mentoring, communication, and leadership. It has been an honor to have worked with them. Christopher Holmes and Elise Didion deserve special recognition. I cannot thank them enough for being wonderful lab mates and two of my best friends. Without them this process would have been much harder and very lonely. v I am so grateful for the patience and support from my family over the last six years. They never complained when I missed countless birthdays and holidays because I was working or studying, instead welcoming me home with open arms every chance they got. My parents and step-parents have been supportive, kind, and forgiving beyond any reasonable expectation. Without them, my education would have been impossible. They believed in me when I couldn’t believe in myself, pushed me to constantly be better and strive for more than I thought I could. I would like to recognize the immense support of my running family. Not only have they been there for me when I needed a friend, but they have listened to me talk about all aspects of graduate school ad nauseum (sometimes for 26.2 miles). I am grateful for the unique perspective and advice that each one of them has provided me over the last two years. In addition to coaching me through three full marathons, countless training runs, and smaller races, they have also coached me throughout the last two years of my PhD, a very different kind of marathon. Samantha Isler deserves more recognition than I can give her here, meeting her dancing in the rain back in high school might be one of the greatest things that has ever happened to me. I cannot imagine my life without her, and I cannot thank her enough for being my best friend for all of these years. She has been there for me through every high and every low, my light in the dark. Lastly, I need to thank my husband, Justin. He has loved me when I was a nightmare, always understood how much my research means to me, and forgiven me when I put him second to science. He has always supported all of my wildest dreams, even when I changed my mind constantly about what those were. Justin is constantly lifting me up, assuring me that I am vi enough, and that I deserve to be where I am. I’m fairly certain that I wouldn’t be able to accomplish anything without him, including this degree.
Load more

Recommended publications
  • New Aspects About Supella Longipalpa (Blattaria: Blattellidae)
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Asian Pac J Trop Biomed 2016; 6(12): 1065–1075 1065 HOSTED BY Contents lists available at ScienceDirect Asian Pacific Journal of Tropical Biomedicine journal homepage: www.elsevier.com/locate/apjtb Review article http://dx.doi.org/10.1016/j.apjtb.2016.08.017 New aspects about Supella longipalpa (Blattaria: Blattellidae) Hassan Nasirian* Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran ARTICLE INFO ABSTRACT Article history: The brown-banded cockroach, Supella longipalpa (Blattaria: Blattellidae) (S. longipalpa), Received 16 Jun 2015 recently has infested the buildings and hospitals in wide areas of Iran, and this review was Received in revised form 3 Jul 2015, prepared to identify current knowledge and knowledge gaps about the brown-banded 2nd revised form 7 Jun, 3rd revised cockroach. Scientific reports and peer-reviewed papers concerning S. longipalpa and form 18 Jul 2016 relevant topics were collected and synthesized with the objective of learning more about Accepted 10 Aug 2016 health-related impacts and possible management of S. longipalpa in Iran. Like the Available online 15 Oct 2016 German cockroach, the brown-banded cockroach is a known vector for food-borne dis- eases and drug resistant bacteria, contaminated by infectious disease agents, involved in human intestinal parasites and is the intermediate host of Trichospirura leptostoma and Keywords: Moniliformis moniliformis. Because its habitat is widespread, distributed throughout Brown-banded cockroach different areas of homes and buildings, it is difficult to control.
    [Show full text]
  • Cockroach Marion Copeland
    Cockroach Marion Copeland Animal series Cockroach Animal Series editor: Jonathan Burt Already published Crow Boria Sax Tortoise Peter Young Ant Charlotte Sleigh Forthcoming Wolf Falcon Garry Marvin Helen Macdonald Bear Parrot Robert E. Bieder Paul Carter Horse Whale Sarah Wintle Joseph Roman Spider Rat Leslie Dick Jonathan Burt Dog Hare Susan McHugh Simon Carnell Snake Bee Drake Stutesman Claire Preston Oyster Rebecca Stott Cockroach Marion Copeland reaktion books Published by reaktion books ltd 79 Farringdon Road London ec1m 3ju, uk www.reaktionbooks.co.uk First published 2003 Copyright © Marion Copeland All rights reserved No part of this publication may be reproduced, stored in a retrieval system or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise without the prior permission of the publishers. Printed and bound in Hong Kong British Library Cataloguing in Publication Data Copeland, Marion Cockroach. – (Animal) 1. Cockroaches 2. Animals and civilization I. Title 595.7’28 isbn 1 86189 192 x Contents Introduction 7 1 A Living Fossil 15 2 What’s in a Name? 44 3 Fellow Traveller 60 4 In the Mind of Man: Myth, Folklore and the Arts 79 5 Tales from the Underside 107 6 Robo-roach 130 7 The Golden Cockroach 148 Timeline 170 Appendix: ‘La Cucaracha’ 172 References 174 Bibliography 186 Associations 189 Websites 190 Acknowledgements 191 Photo Acknowledgements 193 Index 196 Two types of cockroach, from the first major work of American natural history, published in 1747. Introduction The cockroach could not have scuttled along, almost unchanged, for over three hundred million years – some two hundred and ninety-nine million before man evolved – unless it was doing something right.
    [Show full text]
  • New Species of Hammerschmidtiella Chitwood, 1932, and Blattophila
    Zootaxa 4226 (3): 429–441 ISSN 1175-5326 (print edition) http://www.mapress.com/j/zt/ Article ZOOTAXA Copyright © 2017 Magnolia Press ISSN 1175-5334 (online edition) https://doi.org/10.11646/zootaxa.4226.3.6 http://zoobank.org/urn:lsid:zoobank.org:pub:77877607-ECE7-455E-A76C-353B16F92296 New species of Hammerschmidtiella Chitwood, 1932, and Blattophila Cobb, 1920, and new geographical records for Severianoia annamensis Van Luc & Spiridonov, 1993 (Nematoda: Oxyurida: Thelastomatoidea) from Cockroaches (Insecta: Blattaria) in Ohio and Florida, U.S.A. RAMON A. CARRENO Department of Zoology, Ohio Wesleyan University, Delaware, Ohio, 43015, USA. E-mail: [email protected] Abstract Two new species of thelastomatid nematodes parasitic in the hindgut of cockroaches are described. Hammerschmidtiella keeneyi n. sp. is described from a laboratory colony of Diploptera punctata (Eschscholtz, 1822) from a facility in Ohio, U. S. A. This species is characterized by having females with a short tail and males smaller than those described from other species. The new species also differs from others in the genus by a number of differing measurements that indicate a distinct identity, including esophageal, tail, and egg lengths as well as the relative position of the excretory pore. Blat- tophila peregrinata n. sp. is described from Periplaneta australasiae (Fabricius, 1775) and Pycnoscelus surinamensis (Linnaeus, 1758) in a greenhouse from Ohio, U.S.A. and from wild P. surinamensis in southern Florida, U.S.A. This spe- cies differs from others in the genus by having a posteriorly directed vagina, vulva in the anterior third of the body, no lateral alae in females, and eggs with an operculum.
    [Show full text]
  • Serial Horizontal Transfer of Vitamin-Biosynthetic Genes Enables the Establishment of New Nutritional Symbionts in Aphids’ Di-Symbiotic Systems
    The ISME Journal (2020) 14:259–273 https://doi.org/10.1038/s41396-019-0533-6 ARTICLE Serial horizontal transfer of vitamin-biosynthetic genes enables the establishment of new nutritional symbionts in aphids’ di-symbiotic systems 1 1 1 2 2 Alejandro Manzano-Marıń ● Armelle Coeur d’acier ● Anne-Laure Clamens ● Céline Orvain ● Corinne Cruaud ● 2 1 Valérie Barbe ● Emmanuelle Jousselin Received: 25 February 2019 / Revised: 24 August 2019 / Accepted: 7 September 2019 / Published online: 17 October 2019 © The Author(s) 2019. This article is published with open access Abstract Many insects depend on obligate mutualistic bacteria to provide essential nutrients lacking from their diet. Most aphids, whose diet consists of phloem, rely on the bacterial endosymbiont Buchnera aphidicola to supply essential amino acids and B vitamins. However, in some aphid species, provision of these nutrients is partitioned between Buchnera and a younger bacterial partner, whose identity varies across aphid lineages. Little is known about the origin and the evolutionary stability of these di-symbiotic systems. It is also unclear whether the novel symbionts merely compensate for losses in Buchnera or 1234567890();,: 1234567890();,: carry new nutritional functions. Using whole-genome endosymbiont sequences of nine Cinara aphids that harbour an Erwinia-related symbiont to complement Buchnera, we show that the Erwinia association arose from a single event of symbiont lifestyle shift, from a free-living to an obligate intracellular one. This event resulted in drastic genome reduction, long-term genome stasis, and co-divergence with aphids. Fluorescence in situ hybridisation reveals that Erwinia inhabits its own bacteriocytes near Buchnera’s. Altogether these results depict a scenario for the establishment of Erwinia as an obligate symbiont that mirrors Buchnera’s.
    [Show full text]
  • Evolution of Oviductal Gestation in Amphibians MARVALEE H
    THE JOURNAL OF EXPERIMENTAL ZOOLOGY 266394-413 (1993) Evolution of Oviductal Gestation in Amphibians MARVALEE H. WAKE Department of Integrative Biology and Museum of Vertebrate Zoology, University of California,Berkeley, California 94720 ABSTRACT Oviductal retention of developing embryos, with provision for maternal nutrition after yolk is exhausted (viviparity) and maintenance through metamorphosis, has evolved indepen- dently in each of the three living orders of amphibians, the Anura (frogs and toads), the Urodela (salamanders and newts), and the Gymnophiona (caecilians). In anurans and urodeles obligate vivi- parity is very rare (less than 1%of species); a few additional species retain the developing young, but nutrition is yolk-dependent (ovoviviparity) and, at least in salamanders, the young may be born be- fore metamorphosis is complete. However, in caecilians probably the majority of the approximately 170 species are viviparous, and none are ovoviviparous. All of the amphibians that retain their young oviductally practice internal fertilization; the mechanism is cloaca1 apposition in frogs, spermato- phore reception in salamanders, and intromission in caecilians. Internal fertilization is a necessary but not sufficient exaptation (sensu Gould and Vrba: Paleobiology 8:4-15, ’82) for viviparity. The sala- manders and all but one of the frogs that are oviductal developers live at high altitudes and are subject to rigorous climatic variables; hence, it has been suggested that cold might be a “selection pressure” for the evolution of egg retention. However, one frog and all the live-bearing caecilians are tropical low to middle elevation inhabitants, so factors other than cold are implicated in the evolu- tion of live-bearing.
    [Show full text]
  • Genetically Modified Baculoviruses for Pest
    INSECT CONTROL BIOLOGICAL AND SYNTHETIC AGENTS This page intentionally left blank INSECT CONTROL BIOLOGICAL AND SYNTHETIC AGENTS EDITED BY LAWRENCE I. GILBERT SARJEET S. GILL Amsterdam • Boston • Heidelberg • London • New York • Oxford Paris • San Diego • San Francisco • Singapore • Sydney • Tokyo Academic Press is an imprint of Elsevier Academic Press, 32 Jamestown Road, London, NW1 7BU, UK 30 Corporate Drive, Suite 400, Burlington, MA 01803, USA 525 B Street, Suite 1800, San Diego, CA 92101-4495, USA ª 2010 Elsevier B.V. All rights reserved The chapters first appeared in Comprehensive Molecular Insect Science, edited by Lawrence I. Gilbert, Kostas Iatrou, and Sarjeet S. Gill (Elsevier, B.V. 2005). All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publishers. Permissions may be sought directly from Elsevier’s Rights Department in Oxford, UK: phone (þ44) 1865 843830, fax (þ44) 1865 853333, e-mail [email protected]. Requests may also be completed on-line via the homepage (http://www.elsevier.com/locate/permissions). Library of Congress Cataloging-in-Publication Data Insect control : biological and synthetic agents / editors-in-chief: Lawrence I. Gilbert, Sarjeet S. Gill. – 1st ed. p. cm. Includes bibliographical references and index. ISBN 978-0-12-381449-4 (alk. paper) 1. Insect pests–Control. 2. Insecticides. I. Gilbert, Lawrence I. (Lawrence Irwin), 1929- II. Gill, Sarjeet S. SB931.I42 2010 632’.7–dc22 2010010547 A catalogue record for this book is available from the British Library ISBN 978-0-12-381449-4 Cover Images: (Top Left) Important pest insect targeted by neonicotinoid insecticides: Sweet-potato whitefly, Bemisia tabaci; (Top Right) Control (bottom) and tebufenozide intoxicated by ingestion (top) larvae of the white tussock moth, from Chapter 4; (Bottom) Mode of action of Cry1A toxins, from Addendum A7.
    [Show full text]
  • Table S4. Phylogenetic Distribution of Bacterial and Archaea Genomes in Groups A, B, C, D, and X
    Table S4. Phylogenetic distribution of bacterial and archaea genomes in groups A, B, C, D, and X. Group A a: Total number of genomes in the taxon b: Number of group A genomes in the taxon c: Percentage of group A genomes in the taxon a b c cellular organisms 5007 2974 59.4 |__ Bacteria 4769 2935 61.5 | |__ Proteobacteria 1854 1570 84.7 | | |__ Gammaproteobacteria 711 631 88.7 | | | |__ Enterobacterales 112 97 86.6 | | | | |__ Enterobacteriaceae 41 32 78.0 | | | | | |__ unclassified Enterobacteriaceae 13 7 53.8 | | | | |__ Erwiniaceae 30 28 93.3 | | | | | |__ Erwinia 10 10 100.0 | | | | | |__ Buchnera 8 8 100.0 | | | | | | |__ Buchnera aphidicola 8 8 100.0 | | | | | |__ Pantoea 8 8 100.0 | | | | |__ Yersiniaceae 14 14 100.0 | | | | | |__ Serratia 8 8 100.0 | | | | |__ Morganellaceae 13 10 76.9 | | | | |__ Pectobacteriaceae 8 8 100.0 | | | |__ Alteromonadales 94 94 100.0 | | | | |__ Alteromonadaceae 34 34 100.0 | | | | | |__ Marinobacter 12 12 100.0 | | | | |__ Shewanellaceae 17 17 100.0 | | | | | |__ Shewanella 17 17 100.0 | | | | |__ Pseudoalteromonadaceae 16 16 100.0 | | | | | |__ Pseudoalteromonas 15 15 100.0 | | | | |__ Idiomarinaceae 9 9 100.0 | | | | | |__ Idiomarina 9 9 100.0 | | | | |__ Colwelliaceae 6 6 100.0 | | | |__ Pseudomonadales 81 81 100.0 | | | | |__ Moraxellaceae 41 41 100.0 | | | | | |__ Acinetobacter 25 25 100.0 | | | | | |__ Psychrobacter 8 8 100.0 | | | | | |__ Moraxella 6 6 100.0 | | | | |__ Pseudomonadaceae 40 40 100.0 | | | | | |__ Pseudomonas 38 38 100.0 | | | |__ Oceanospirillales 73 72 98.6 | | | | |__ Oceanospirillaceae
    [Show full text]
  • Blattabacterium Genome: Structure, Function, and Evolution Austin Alleman
    University of Texas at Tyler Scholar Works at UT Tyler Biology Theses Biology Spring 6-5-2014 Blattabacterium Genome: Structure, Function, and Evolution Austin Alleman Follow this and additional works at: https://scholarworks.uttyler.edu/biology_grad Part of the Biology Commons Recommended Citation Alleman, Austin, "Blattabacterium Genome: Structure, Function, and Evolution" (2014). Biology Theses. Paper 20. http://hdl.handle.net/10950/215 This Thesis is brought to you for free and open access by the Biology at Scholar Works at UT Tyler. It has been accepted for inclusion in Biology Theses by an authorized administrator of Scholar Works at UT Tyler. For more information, please contact [email protected]. BLATTABACTERIUM GENOME: STRUCTURE, FUNCTION, AND EVOLUTION by AUSTIN ALLEMAN A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science Department of Biology Srini Kambhampati, Ph. D., Committee Chair College of Arts and Sciences The University of Texas at Tyler May 2014 © Copyright by Austin Alleman 2014 All rights reserved Acknowledgements I would like to thank the Sam A. Lindsey Endowment, without whose support this research would not have been possible. I would also like to thank my research advisor, Dr. Srini Kambhampati, for his knowledge, insight, and patience; and my committee, Dr. John S. Placyk, Jr. and Dr. Blake Bextine, for their assistance and feedback. “It is the supreme art of the teacher to awaken joy in creative expression and knowledge.” --Albert Einstein Table of Contents Chapter
    [Show full text]
  • Moniliformis Moniliformis Infection Has No Effect on Some Behaviors of the Cockroach Diploptera Punctata Author(S): Zachary Allely, Janice Moore and Nicholas J
    Moniliformis moniliformis Infection Has No Effect on Some Behaviors of the Cockroach Diploptera punctata Author(s): Zachary Allely, Janice Moore and Nicholas J. Gotelli Source: The Journal of Parasitology, Vol. 78, No. 3 (Jun., 1992), pp. 524-526 Published by: The American Society of Parasitologists Stable URL: http://www.jstor.org/stable/3283658 . Accessed: 04/05/2013 10:55 Your use of the JSTOR archive indicates your acceptance of the Terms & Conditions of Use, available at . http://www.jstor.org/page/info/about/policies/terms.jsp . JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact [email protected]. The American Society of Parasitologists is collaborating with JSTOR to digitize, preserve and extend access to The Journal of Parasitology. http://www.jstor.org This content downloaded from 132.198.40.214 on Sat, 4 May 2013 10:55:35 AM All use subject to JSTOR Terms and Conditions RESEARCH NOTES J. Parasitol., 78(3), 1992, p. 524-526 ? American Society of Parasitologists 1992 Moniliformismoniliformis Infection Has No Effect on Some Behaviorsof the CockroachDiploptera punctata Zachary Allely, Janice Moore, and Nicholas J. Gotelli*, Departmentof Biology,Colorado State University,Fort Collins,Colorado 80523; and *Departmentof Zoology,University of Oklahoma,Norman, Oklahoma 73019 ABSTRACT: The behaviorof the cockroachDiploptera Experiments were conducted under 2 light punctataparasitized with the acanthocephalanMonil- conditions: white light and red light (Carmichael was examined for iformis moniliformis parasite-in- and Moore, 1991).
    [Show full text]
  • Parallel and Gradual Genome Erosion in the Blattabacterium Endosymbionts of Mastotermes Darwiniensis and Cryptocercus Wood Roaches
    GBE Parallel and Gradual Genome Erosion in the Blattabacterium Endosymbionts of Mastotermes darwiniensis and Cryptocercus Wood Roaches Yukihiro Kinjo1,2,3,4, Thomas Bourguignon3,5,KweiJunTong6, Hirokazu Kuwahara2,SangJinLim7, Kwang Bae Yoon7, Shuji Shigenobu8, Yung Chul Park7, Christine A. Nalepa9, Yuichi Hongoh1,2, Moriya Ohkuma1,NathanLo6,*, and Gaku Tokuda4,* 1Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Japan 2Department of Life Science and Technology, Tokyo Institute of Technology, Tokyo, Japan 3Okinawa Institute of Science and Technology, Graduate University, Okinawa, Japan 4Tropical Biosphere Research Center, University of the Ryukyus, Okinawa, Japan 5Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Prague, Czech Republic 6School of Life and Environmental Sciences, University of Sydney, NSW, Australia 7Division of Forest Science, Kangwon National University, Chuncheon, Republic of Korea 8National Institute for Basic Biology, NIBB Core Research Facilities, Okazaki, Japan 9Department of Entomology, North Carolina State University, Raleigh, North Carolina, USA *Corresponding authors: E-mails: [email protected]; [email protected]. Accepted: May 29, 2018 Data deposition: This project has been deposited in the International Nucleotide Sequence Database (GenBank/ENA/DDBJ) under the accession numbers given in Table 1. Abstract Almost all examined cockroaches harbor an obligate intracellular endosymbiont, Blattabacterium cuenoti.Onthebasisof genome content, Blattabacterium has been inferred to recycle nitrogen wastes and provide amino acids and cofactors for its hosts. Most Blattabacterium strains sequenced to date harbor a genome of 630 kbp, with the exception of the termite Mastotermes darwiniensis (590 kbp) and Cryptocercus punctulatus (614 kbp), a representative of the sister group of termites. Such genome reduction may have led to the ultimate loss of Blattabacterium in all termites other than Mastotermes.
    [Show full text]
  • Identifying Lethal Temperatures Targeting Immature Life Stage Control of Spotted Wing Drosophila
    AGRICULTURAL RESEARCH FOUNDATION FINAL REPORT FUNDING CYCLE 2015 – 2017 TITLE: Identifying Lethal Temperatures Targeting Immature Life Stage Control of Spotted Wing Drosophila RESEARCH LEADER: Vaughn Walton COOPERATORS: Daniel Dalton, Riki York SUMMARY: Survival of spotted wing drosophila (D. suzukii, SWD) larvae to adulthood was evaluated after exposure to temperatures ranging 29-49°C in a laboratory thermal gradient bench for 60 minutes. Vials filled with pre-made fly diet up to approximately 1.5 cm from the bottom were used as oviposition media inside the gradient wells. Forty-three vials were prepared each day for 4 consecutive days. Five adult females were placed in each of 36 vials for 24 hours for oviposition to occur, seven vials were held as controls without flies. Upon removal, adult flies were placed in ethanol. Four ages were tested between 1-4-day old egg/larvae (i.e. 1 day old, 2 days old, 3 days old, and four days old). Sample vials were examined for oviposition success. Immature flies exposed temperatures of 32-35°C had the highest survival to adulthood. Above 38°C survival to adulthood with no survival at 41°C and 49°C, and a few surviving to adulthood under 45°C. An additional trial was conducted to evaluate the potential for thermal conditioning to improve larval survival at higher temperatures. For this study, four-day-old larvae were subject to 30, 60, and 90 minutes at 35°C, then allowed to cool to room temperature (22°C) for 60 minutes prior to placement in the thermal bench. These trials were used to determine how heat may reduce successful development to adulthood, leading to better management practices in the field.
    [Show full text]
  • Running Speed and Food Intake of the Matrotrophic Viviparous Cockroach Diploptera Punctata (Blattodea: Blaberidae) During Gestation
    ZOBODAT - www.zobodat.at Zoologisch-Botanische Datenbank/Zoological-Botanical Database Digitale Literatur/Digital Literature Zeitschrift/Journal: Entomologie heute Jahr/Year: 2014 Band/Volume: 26 Autor(en)/Author(s): Greven Hartmut, Floßdorf David, Köthe Janine, List Fabian, Zwanzig Nadine Artikel/Article: Running Speed and Food Intake of the Matrotrophic Viviparous Cockroach Diploptera punctata (Blattodea: Blaberidae) during Gestation. Laufgeschwindigkeit und Nahrungsaufnahme der matrotroph viviparen Schabe Diploptera punctata (Blattodea: Blaberidae) während der Trächtigkeit 53-72 Running speed and food intake of Diploptera punctata during gestation 53 Entomologie heute 26 (2014): 53-72 Running Speed and Food Intake of the Matrotrophic Viviparous Cockroach Diploptera punctata (Blattodea: Blaberidae) during Gestation Laufgeschwindigkeit und Nahrungsaufnahme der matrotroph viviparen Schabe Diploptera punctata (Blattodea: Blaberidae) während der Trächtigkeit HARTMUT GREVEN, DAVID FLOSSDORF, JANINE KÖTHE, FABIAN LIST & NADINE ZWANZIG Summary: Diploptera punctata is the only cockroach, which has been clearly characterized as matro- trophic viviparous. Our observations on courtship and mating generally confi rm the data from the literature. Courtship and mating correspond to type I (male offers himself under wing fl uttering, female mounts the male, nibbles on his tergal glands, dismounts, turns to achieve the fi nal mating position, i.e. abdomen to abdomen, heads in the opposite direction. We document photographic ally mating and courtship of fully sklerotized, sexually experienced males with teneral females imme- diately after the last moult, and with fully sclerotized females several hours after the fi nal moult. Effects of sexual dimorphism (females are larger than males) and pregnancy (females gain weight) became apparent from the running speed cockroaches reached, when disturbed. Males and females ran signifi cantly faster during daytime than at night, but males ran always faster than females.
    [Show full text]