Materialsuplementario

MATERIAL SUPLEMENTARIO Figura suplementaria 1. Alineamiento de la secuencia nucleotídica del primer exón de Catsper1. Las regiones que contienen inserciones o deleciones (indels) son indicadas. Cricetulus griseus se incluye en el alineamiento porque fue utilizado como linaje externo en los análisis evolutivos.

1 2 3

4 5 6 7

8 9 10 11 12

13 14 15 16 17 18 19 20 21

22 23 24 25 Figura suplementaria 2. Diagnósticos de regresión entre la longitud de Catsper1 y la masa relativa de testículo. El punto número 4 se corresponde con la especie Mastomys natalensis, la cual se consideró un outlier por mostrar una alta desviación respecto a la distribución de los datos. Aunque aquí no se muestra, se observaron los mismos resultados cuando se utilizaron los parámetros de velocidad como variables predictoras.

Residuales vs Ajustados Normal Q-Q

Residuales Residuales estandarizados Residuales

Valores ajustados Cuantiles teóricos

Escala - localización Diagrama de dispersión

terminal de de Catsper1 terminal

Raíz cuadrada de los los de Raíz cuadrada

Residuales estandarizados Residuales Tamaño de la región Tamaño la región de N

Valores ajustados Masa relativa de testículos Rectitud (%) Velocidad curvilinea (μm/s) espermática. Figura suplementaria 3. Gráficas representando la asociación entre la longitud de la región N Longitud del N Longituddel Longitud del N Longituddel - - terminal determinal Catsper1

terminal determinal Catsper1

Amplitud del desplazamiento lateral de la cabeza (%) Velocidad rectilinea (μm/s) Longitud del N Longituddel Longitud del N Longituddel - terminal determinal Catsper1 - terminal determinal Catsper1

Velocidad de la trayectoria Frecuencia de batido (Hz) media (μm/s) Longitud del N Longituddel N Longituddel - terminal terminal de CatSper1 ylos parámet - - terminal determinal Catsper1 determinal Catsper1

Linearidad (%) Longitud del N Longituddel ros de velocidad - terminal determinal Catsper1

Figura suplementaria 4. Alineamiento de la secuencia nucleotídica de Pkdrej. En la primera página se muestra el alineamiento para las especies de múridos, y en la segunda página el alineamiento de las especies de cricétidos.

Figura suplementaria 5. Los cladogramas de roedores que se usaron para los análisis evolutivos. Los análisis se llevaron a cabo considerando las dos posibles topologías derivadas de la politomía de las subespecies de Mus musculus.

Muridae

Cricetidae

Figura suplementaria 6. Alineamiento de la secuencia nucleotídica de Izumo1 en roedores múridos.

Figura suplementaria 7. Alineamiento de la secuencia nucleotídica de Cd9 en roedores múridos.

Figura suplementaria 8. Distribución de los valores de APEX para las tres especies analizadas.

Densidad

Valor de APEX Figura suplementaria 9. Análisis de robustez para péptidos con detección exclusiva en las espcies.

Número de casos

Especie Mus musculus Mus spretus Compartidos Exclusivos Péptidos No significativos Significativos Total No significativos Significativos Total TOTAL Compartidos 44149 3940 48089 54406 3970 58376 106465

Densidad Exclusivos 3570 666 4236 4546 561 5107 9343 TOTAL 47719 4606 52325 58952 4531 63483 115808

Número de casos

Compartidos Especie Mus musculus Mus spicilegus Exclusivos Péptidos No significativos Significativos Total No significativos Significativos Total TOTAL Compartidos 43344 4887 48231 49811 1860 51671 99902

Densidad Exclusivos 3424 497 3921 4860 621 5481 9402 TOTAL 46768 5384 52152 54671 2481 57152 109304

Número de casos

Número de casos Compartidos

Exclusivos Especie Mus spretus Mus spicilegus Péptidos No significativos Significativos Total No significativos Significativos Total TOTAL Compartidos 72158 8333 80491 38004 4567 42571 123062

Densidad Exclusivos 3941 262 4203 3808 299 4107 8310 TOTAL 76099 8595 84694 41812 4866 46678 131372

Número de casos Tabla suplementaria 1. Lista de proteínas analizadas y procesos reproductivos a los que se asocian. Proteína Nombre de la proteína Proceso reproductivo Acrbp Acrosin binding protein Reacción acrosómica Afaf Acrosome formation- associated factor Reacción acrosómica Akt1 RAC-alpha serine/threonine-protein kinase Reacción acrosómica Cacna1h Voltage-dependent T-type calcium channel subunit alpha-1H Reacción acrosómica Cd46 CD46 antigen, complement regulatory protein Reacción acrosómica Enkur Enkurin Reacción acrosómica Glra1 Glycine receptor subunit alpha 1 Reacción acrosómica Itpr1 Inositol 1,4,5-trisphosphate receptor type 1 Reacción acrosómica Pdpk1 3-phosphoinositide dependent protein kinase 1 Reacción acrosómica Pik3c3 Phosphoinositide-3-kinase, class 3 Reacción acrosómica Pik3r3 Phosphatidil inositol 3 kinasa regulatory subunit 3 Reacción acrosómica Pla2g1b Phospholipase A2 Reacción acrosómica Plcd4 Phospholipase C delta 4 Reacción acrosómica Prkcz Protein kinase C zeta type Reacción acrosómica Ptpn1 Tyrosine-protein phosphatase non-receptor type 1 Reacción acrosómica Syt6 Synaptotagmin-6 Reacción acrosómica Syt8 Synaptotagmin-8 Reacción acrosómica Trim36 E3 ubiquitin-protein ligase Trim36 Reacción acrosómica Trpc2 Short transient receptor potential channel 2 Reacción acrosómica Vcp Transitional endoplasmic reticulum ATPase Reacción acrosómica Adcy2 Adenylate cyclase type 2 Capacitación Adcy8 Adenylate cyclase type 8 Capacitación Cabyr Calcium-binding tyrosine phosphorilation-regulated protein Capacitación Cacna1c Voltage-dependent L-type calcium channel subunit alpha-1C Capacitación Calca Calcitonin-related polypeptide, alpha Capacitación Calm1 Calmodulin Capacitación Dld Dihydrolipoamide dehydrogenase Capacitación Fsip2 Fibrous sheath-interacting protein 2 Capacitación Pcsk4 Proprotein convertase subtilisin/kexin type 4 Capacitación Pde4d Phospodiesterase 4d Capacitación Plscr2 Phospholipid scramblase 2 Capacitación Ppp3r2 Calcineurin subunit B type 2 Capacitación Prkaca Sperm -specific protein kinase A catalytic subunit Capacitación Pyk2 Protein-tyrosine kinase 2-beta Capacitación Rab14 Ras-related protein Rab-14 Capacitación Sacy Soluble adenylate cyclase Capacitación Slo3 Potassium channel subfamily U member 1 Capacitación Tex101 Testis-expressed protein 101 Capacitación Trpc3 Short transient receptor potential channel 3 Capacitación Pebp1 Phosphatidylethanolamine-binding protein 1 Capacitación Svs2 Semenogelin-1 Capacitación Aldoa1 Fructosa bisphosphate aldolase Metabolismo Atp5b ATP synthetase beta subunit, mitochondrial Metabolismo Cox6b2 Cytochrome c oxidase subunit 6B2 Metabolismo Csl Cytrate synthase-like gene Metabolismo Cyct Cytochrome c, testis-specific Metabolismo Dbil5 Diazepam-binding inhibitor-like 5 Metabolismo Dlat Dihydrolipoyllysine-residue acetyltransferase Metabolismo Eno1 Enolase 1 Metabolismo G6pd2 Glucose-6-phosphate 1-dehydrogenase 2 Metabolismo Gapdhs Glyceraldehyde-3-phosphate dehydrogenase, spermatogenic Metabolismo Glut3 Solute carrier family 2, facilitated glucose transporter member 3 Metabolismo Gpd2 Glycerol phospate deshydrogenase 2 Metabolismo Gpi1 Glucose phosphate isomerase Metabolismo Hk1s Hexokinase-1, spermatogenic cell-specific Metabolismo Idh1 Isocitrate dehydrogenase [NADP] cytoplasmic Metabolismo Ldhc L-lactate dehydrogenase C chain Metabolismo Mpi Mannose-6-phosphate isomerase Metabolismo Oxct2a Succinyl-CoA:3-ketoacid-coenzyme A transferase 2A, mitochondrial Metabolismo Pdha2 Piruvate dehydrogenase A2 Metabolismo Pfkfb4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 Metabolismo Pgam2 Phosphoglycerate mutase 2 Metabolismo Proteína Nombre de la proteína Proceso reproductivo Pgk2 Phosphoglycerate kinase 2 Metabolismo Pkm2 Pyruvate kinase isozymes M1/M2 Metabolismo Ak1 Adenylate kinase 1 Motilidad Akap110 A- kinase anchor protein 3 Motilidad Akap82 A- kinase anchor protein 82 Motilidad Atp1a4 Sodium/potassium-transporting ATPase subunit alpha-4 Motilidad Car4 Carbonic anhydrase-4 Motilidad Catsper1 Cation channel sperm associated protein 1 Motilidad CatSper2 Cation channel sperm associated protein 2 Motilidad Catsper3 Cation channel sperm associated protein 3 Motilidad Catsper4 Cation channel sperm associated protein 4 Motilidad CatsperG Cation channel sperm associated protein G Motilidad Chdh Choline dehydrogenase Motilidad Chrna7 Neuronal acetylcholine receptor subunit alpha-7 Motilidad Dnah12 Dynein heavy chain 12 Motilidad Gas8 Growth arrest-specific protein 8 Motilidad Ift88 Intraflagellar transport protein 88 homolog Motilidad Neurl1a Neuralized-like protein 1A Motilidad Nhe1 Sodium/hydrogen exchanger 1 Motilidad Nhe10 Sodium/hydrogen exchanger 10 Motilidad Nhe5 Sodium/hydrogen exchanger 5 Motilidad Odf4 Outer dense fiber protein-4 Motilidad Pmca4 Plasma membrane calcium/calmodulin-dependent calcium ATPase, isoform 4 Motilidad Rhpn1 Rhophilin-1 Motilidad Ropn1 Ropporin-1 Motilidad Slc25a1 Solute carrier family 25 member 1 Motilidad Smcp Sperm mitochondria-associated cysteine-rich protein. Motilidad Smky Sperm motility Kinase Y Motilidad Smok2a Sperm motility kinase 2A Motilidad Smok2b Sperm motility kinase 2B Motilidad Smok4a Sperm motility kinase 4A Motilidad Spag6 Sperm associated antigen 6 Motilidad Tctex1 Dynein light chain Tctex-type 1 Motilidad Tctex5 Dynein light chain Tctex-type 5 Motilidad Tekt3 Tektin-3 Motilidad Tekt4 Tektin-4 Motilidad Boll Boule-like protein Espermatogénesis Ccna1 Cyclin A1 Espermatogénesis Celf3 CUGBP Elav-like family member 3 Espermatogénesis Cttn Cotractine Espermatogénesis Dazla Deleted in azoospermia-like Espermatogénesis Ddx4 Probable ATP-dependent RNA helicase Espermatogénesis Dnmt3l DNA (cytosine-5)-methyltransferase 3-like Espermatogénesis Etv5 ETS translocation variant 5 Espermatogénesis Fkbp6 Peptidyl-prolyl cis-trans isomerase FKBP6 Espermatogénesis Fscb Fibrous sheat CABYR-binding protein Espermatogénesis H1fnt Testis-specific H1 histone Espermatogénesis Hils1 Histone H1-like protein in spermatids 1 Espermatogénesis Krt9 Keratin-9 Espermatogénesis Prm1 Protamine 1 Espermatogénesis Prm2 Protamine 2 Espermatogénesis Pvrl2 Nectin 2 Espermatogénesis Rsph1 Radial spoke head 1 homolog Espermatogénesis Sept4 Septin-4 Espermatogénesis Spata16 Spermatogenesis-associated protein 16 Espermatogénesis Spata24 Spermatogenesis-associated protein 24 Espermatogénesis Spatc1 Speriolin Espermatogénesis Sptrx1 Thioredoxin domain-containing protein 2 Espermatogénesis Stx2 Sintaxin-2 Espermatogénesis Taf7l Transcription initiation factor TFIID subunit 7-like Espermatogénesis Tekt2 Tektin-2 Espermatogénesis Tnp1 Transition protein-1 Espermatogénesis Tnp2 Transition protein-2 Espermatogénesis Proteína Nombre de la proteína Proceso reproductivo Tssk1 Testis-specific serine/threonine-protein kinase-1 Espermatogénesis Tssk2 Testis-specific serine/threonine-protein kinase-2 Espermatogénesis Tssk6 Testis-specific serine/threonine-protein kinase-6 Espermatogénesis Vdac3 Voltage-dependent-anion channel mitochondrial Espermatogénesis Dpep3 Dipeptidase 3 Espermatogénesis Pdia3 Disulfide isomerase A3 Espermatogénesis Ace Angiotensin-converting enzyme Interacción espermatozoide-óvulo Acrv1 Acrosomal protein SP-10 Interacción espermatozoide-óvulo Adam18 A disintegrin and metallopeptidase domain 18 Interacción espermatozoide-óvulo Adam1a A disintegrin and metallopeptidase domain 1a Interacción espermatozoide-óvulo Adam1b A disintegrin and metallopeptidase domain 1b Interacción espermatozoide-óvulo Adam2 A disintegrin and metallopeptidase domain 2 Interacción espermatozoide-óvulo Adam24 A disintegrin and metallopeptidase domain 24 Interacción espermatozoide-óvulo Adam3 A disintegrin and metallopeptidase domain 3 Interacción espermatozoide-óvulo Adam32 A disintegrin and metallopeptidase domain 32 Interacción espermatozoide-óvulo Arsa Arysulfatase A Interacción espermatozoide-óvulo Atp8b3 ATPase class I type 8B member 3 Interacción espermatozoide-óvulo B4galt1 Beta-1,4 galactosyltransferase 1 Interacción espermatozoide-óvulo Bsg Basigin Interacción espermatozoide-óvulo Clgn Calmegin Interacción espermatozoide-óvulo Crisp1 Cysteine-rich secretory protein 1 Interacción espermatozoide-óvulo Crisp2 Cysteine-rich secretory protein 2 Interacción espermatozoide-óvulo Crisp4 Cysteine-rich secretory protein 4 Interacción espermatozoide-óvulo Ctsl1 Cysteine-specific cathepsin Interacción espermatozoide-óvulo Dkkl1 Dickkofp-like 1 Interacción espermatozoide-óvulo Hyal5 Hyaluronidase-5 Interacción espermatozoide-óvulo Izumo1 Izumo sperm-egg fusion protein 1 Interacción espermatozoide-óvulo Mgea5 Beta-N-acetylhexosaminidase Interacción espermatozoide-óvulo Ph20 Hyaluronidase PH-20 Interacción espermatozoide-óvulo Pkdrej Polycystic kidney disease and receptor for egg jelly-related protein Interacción espermatozoide-óvulo Sed1 Bimotif EGF Repeat and Discoidin-Domain Protein-1 Interacción espermatozoide-óvulo Slxl1 Putative novel protein similar to Xlr-related, meiosis regulated Xmr Interacción espermatozoide-óvulo Sp56 Zona pellucida sperm-binding protein 3 receptor Interacción espermatozoide-óvulo Spaca3 Sperm acrosome membrane-associated protein 3 Interacción espermatozoide-óvulo Spesp1 Sperm equatorial segment protein 1 Interacción espermatozoide-óvulo Tcte1 T-complex-associated testis-expressed protein 1 Interacción espermatozoide-óvulo Tmem190 Transmembrane protein 190 Interacción espermatozoide-óvulo Zan Zonadhesin Interacción espermatozoide-óvulo Zpbp1 Zona pellucida binding protein-1 Interacción espermatozoide-óvulo Zpbp2 Zona pellucida binding protein-2 Interacción espermatozoide-óvulo Tabla suplementaria 2. Valores para las tasas de cambios no sinónimos (dN), sinónimos (dS) y ω (dN/dS) estimados para las proteínas del espermatozoide. Las proteínas con ω > 0.5 son representadas en negrita. Proteína Nombre de la proteína Proceso reproductivo dN dS ω Crisp1 Cysteine-rich secretory protein 1 Interacción espermatozoide-óvulo 0,553 0,477 1,165 Fscb Fibrous sheat CABYR-binding protein Espermatogénesis 0,032 0,028 1,151 Slxl1 Putative novel protein similar to Xlr-related, meiosis regulated Xmr Interacción espermatozoide-óvulo 0,065 0,057 1,133 Smcp Sperm mitochondria-associated cysteine-rich protein. Motilidad 0,489 0,433 1,130 Hils1 Histone H1-like protein in spermatids 1 Espermatogénesis 0,218 0,198 1,103 Tmem190 Transmembrane protein 190 Interacción espermatozoide-óvulo 0,087 0,081 1,074 Izumo1 Izumo sperm-egg fusion protein 1 Interacción espermatozoide-óvulo 0,226 0,298 0,758 Sp56 Zona pellucida sperm-binding protein 3 receptor Interacción espermatozoide-óvulo 0,145 0,193 0,752 Cd46 CD46 antigen, complement regulatory protein Reacción acrosómica 0,130 0,181 0,719 Adam24 A disintegrin and metallopeptidase domain 24 Interacción espermatozoide-óvulo 0,150 0,211 0,712 CatSper2 Cation channel sperm associated protein 2 Motilidad 0,149 0,216 0,690 Spesp1 Sperm equatorial segment protein 1 Interacción espermatozoide-óvulo 0,141 0,206 0,683 Krt9 Keratin-9 Espermatogénesis 0,131 0,197 0,665 Sptrx1 Thioredoxin domain-containing protein 2 Espermatogénesis 0,203 0,323 0,630 Ph20 Hyaluronidase PH-20 Interacción espermatozoide-óvulo 0,137 0,221 0,623 Acrv1 Acrosomal protein SP-10 Interacción espermatozoide-óvulo 0,094 0,151 0,622 Svs2 Semenogelin-1 Capacitación 0,186 0,308 0,604 Car4 Carbonic anhydrase-4 Motilidad 0,176 0,302 0,582 H1fnt Testis-specific H1 histone Espermatogénesis 0,136 0,237 0,572 G6pd2 Glucose-6-phosphate 1-dehydrogenase 2 Metabolismo 0,074 0,139 0,537 Afaf Acrosome formation- associated factor Reacción acrosómica 0,132 0,250 0,528 Taf7l Transcription initiation factor TFIID subunit 7-like Espermatogénesis 0,145 0,277 0,524 Smky Motilidad Kinase Y Motilidad 0,146 0,282 0,516 Smok2b Motilidad kinase 2B Motilidad 0,495 0,982 0,504 Mpi Mannose-6-phosphate isomerase Metabolismo 0,067 0,133 0,500 Smok4a Motilidad kinase 4A Motilidad 0,413 0,828 0,498 Smok2a Motilidad kinase 2A Motilidad 0,471 0,945 0,498 CatsperG Cation channel sperm associated protein G Motilidad 0,140 0,294 0,476 Hyal5 Hyaluronidase-5 Interacción espermatozoide-óvulo 0,103 0,218 0,473 Adam32 A disintegrin and metallopeptidase domain 32 Interacción espermatozoide-óvulo 0,121 0,259 0,466 Catsper4 Cation channel sperm associated protein 4 Motilidad 0,095 0,461 0,461 Fsip2 Fibrous sheath-interacting protein 2 Capacitación 0,010 0,022 0,457 Proteína Nombre de la proteína Proceso reproductivo dN dS ω Tex101 Testis-expressed protein 101 Capacitación 0,112 0,251 0,447 Cabyr Calcium-binding tyrosine phosphorilation-regulated protein Capacitación 0,064 0,147 0,435 Tctex5 Dynein light chain Tctex-type 5 Motilidad 0,055 0,128 0,432 Prm1 Protamine 1 Espermatogénesis 0,055 0,128 0,431 Odf4 Outer dense fiber protein-4 Motilidad 0,115 0,284 0,404 Catsper1 Cation channel sperm associated protein 1 Motilidad 0,175 0,434 0,404 Adam3 A disintegrin and metallopeptidase domain 3 Interacción espermatozoide-óvulo 0,118 0,297 0,399 Zan Zonadhesin Interacción espermatozoide-óvulo 0,018 0,046 0,392 Adam18 A disintegrin and metallopeptidase domain 18 Interacción espermatozoide-óvulo 0,107 0,275 0,389 Spatc1 Speriolin Espermatogénesis 0,012 0,032 0,380 Cox6b2 Cytochrome c oxidase subunit 6B2 Metabolismo 0,040 0,108 0,374 Crisp2 Cysteine-rich secretory protein 2 Interacción espermatozoide-óvulo 0,089 0,246 0,359 Adam2 A disintegrin and metallopeptidase domain 2 Interacción espermatozoide-óvulo 0,089 0,249 0,357 Pkdrej Polycystic kidney disease and receptor for egg jelly-related protein Interacción espermatozoide-óvulo 0,102 0,286 0,355 Adam1a A disintegrin and metallopeptidase domain 1a Interacción espermatozoide-óvulo 0,104 0,304 0,343 Slo3 Potassium channel subfamily U member 1 Capacitación 0,006 0,018 0,336 Pik3c3 Phosphoinositide-3-kinase, class 3 Reacción acrosómica 0,012 0,004 0,334 Tctex1 Dynein light chain Tctex-type 1 Motilidad 0,056 0,168 0,332 Spaca3 Sperm acrosome membrane-associated protein 3 Interacción espermatozoide-óvulo 0,057 0,176 0,326 Prm2 Protamine 2 Espermatogénesis 0,045 0,137 0,325 Syt8 Synaptotagmin-8 Reacción acrosómica 0,056 0,189 0,295 Pgk2 Phosphoglycerate kinase 2 Metabolismo 0,058 0,199 0,293 Plcd4 Phospholipase C delta 4 Reacción acrosómica 0,062 0,215 0,290 Pla2g1b Phospholipase A2 Reacción acrosómica 0,069 0,253 0,271 Aldoa1 Fructosa bisphosphate aldolase Metabolismo 0,007 0,025 0,264 Ldhc L-lactate dehydrogenase C chain Metabolismo 0,056 0,213 0,263 Adam1b A disintegrin and metallopeptidase domain 1b Interacción espermatozoide-óvulo 0,011 0,043 0,261 Dpep3 Dipeptidase 3 Espermatogénesis 0,054 0,219 0,245 Zpbp1 Zona pellucida binding protein-1 Interacción espermatozoide-óvulo 0,030 0,123 0,245 Zpbp2 Zona pellucida binding protein-2 Interacción espermatozoide-óvulo 0,079 0,323 0,245 Rsph1 Radial spoke head 1 homolog Espermatogénesis 0,066 0,273 0,242 Ppp3r2 Calcineurin subunit B type 2 Capacitación 0,048 0,202 0,239 Tnp2 Transition protein-2 Espermatogénesis 0,052 0,220 0,238 Dnmt3l DNA (cytosine-5)-methyltransferase 3-like Espermatogénesis 0,069 0,291 0,236 Proteína Nombre de la proteína Proceso reproductivo dN dS ω Itpr1 Inositol 1,4,5-trisphosphate receptor type 1 Reacción acrosómica 0,132 0,582 0,228 Tcte1 T-complex-associated testis-expressed protein 1 Interacción espermatozoide-óvulo 0,058 0,255 0,226 Tekt3 Tektin-3 Motilidad 0,055 0,253 0,216 Pmca4 Plasma membrane calcium/calmodulin-dependent calcium ATPase, isoform 4 Motilidad 0,050 0,233 0,216

Pde4d Phospodiesterase 4d Capacitación 0,018 0,004 0,202 Bsg Basigin Interacción espermatozoide-óvulo 0,061 0,300 0,202 Tnp1 Transition protein-1 Espermatogénesis 0,019 0,098 0,189 Atp8b3 ATPase class I type 8B member 3 Interacción espermatozoide-óvulo 0,064 0,341 0,189 Enkur Enkurin Reacción acrosómica 0,061 0,333 0,183 Plscr2 Phospholipid scramblase 2 Capacitación 0,056 0,307 0,182 B4galt1 Beta-1,4 galactosyltransferase 1 Interacción espermatozoide-óvulo 0,034 0,190 0,181 Oxct2a Succinyl-CoA:3-ketoacid-coenzyme A transferase 2A, mitochondrial Metabolismo 0,070 0,387 0,181 Vdac3 Voltage-dependent-anion channel mitochondrial Espermatogénesis 0,022 0,123 0,180 Catsper3 Cation channel sperm associated protein 3 Motilidad 0,078 0,435 0,179 Dkkl1 Dickkofp-like 1 Interacción espermatozoide-óvulo 0,059 0,339 0,175 Acrbp Acrosin binding protein Reacción acrosómica 0,042 0,242 0,172 Akap110 A- kinase anchor protein 3 Motilidad 0,040 0,237 0,170 Spata16 Espermatogénesis-associated protein 16 Espermatogénesis 0,041 0,257 0,161 Clgn Calmegin Interacción espermatozoide-óvulo 0,046 0,290 0,160 Sed1 Bimotif EGF Repeat and Discoidin-Domain Protein-1 Interacción espermatozoide-óvulo 0,035 0,222 0,159 Trpc2 Short transient receptor potential channel 2 Reacción acrosómica 0,039 0,260 0,151 Tssk1 Testis-specific serine/threonine-protein kinase-1 Espermatogénesis 0,017 0,113 0,151 Crisp4 Cysteine-rich secretory protein 4 Interacción espermatozoide-óvulo 0,059 0,399 0,149 Tekt4 Tektin-4 Motilidad 0,049 0,337 0,145 Dlat Dihydrolipoyllysine-residue acetyltransferase Metabolismo 0,031 0,215 0,143 Chdh Choline dehydrogenase Motilidad 0,018 0,127 0,141 Gapdhs Glyceraldehyde-3-phosphate dehydrogenase, spermatogenic Metabolismo 0,046 0,332 0,140 Calca Calcitonin-related polypeptide, alpha Capacitación 0,020 0,104 0,139 Dnah12 Dynein heavy chain 12 Motilidad 0,033 0,242 0,136 Akap82 A- kinase anchor protein 82 Motilidad 0,030 0,223 0,133 Tekt2 Tektin-2 Espermatogénesis 0,033 0,249 0,131 Ctsl1 Cysteine-specific cathepsin Interacción espermatozoide-óvulo 0,033 0,253 0,130 Atp1a4 Sodium/potassium-transporting ATPase subunit alpha-4 Motilidad 0,035 0,269 0,130 Proteína Nombre de la proteína Proceso reproductivo dN dS ω Rhpn1 Rhophilin-1 Motilidad 0,040 0,312 0,126 Ropn1 Ropporin-1 Motilidad 0,023 0,186 0,125 Arsa Arysulfatase A Interacción espermatozoide-óvulo 0,023 0,190 0,122 Ptpn1 Tyrosine-protein phosphatase non-receptor type 1 Reacción acrosómica 0,036 0,296 0,121 Dbil5 Diazepam-binding inhibitor-like 5 Metabolismo 0,029 0,239 0,120 Nhe10 Sodium/hydrogen exchanger 10 Motilidad 0,054 0,451 0,119 Pdha2 Piruvate dehydrogenase A2 Metabolismo 0,036 0,306 0,119 Ace Angiotensin-converting enzyme Interacción espermatozoide-óvulo 0,033 0,299 0,111 Ccna1 Cyclin A1 Espermatogénesis 0,022 0,199 0,111 Pdpk1 3-phosphoinositide dependent protein kinase 1 Reacción acrosómica 0,011 0,103 0,106 Glut3 Solute carrier family 2, facilitated glucose transporter member 3 Metabolismo 0,029 0,287 0,102 Gpi1 Glucose phosphate isomerase Metabolismo 0,031 0,306 0,100 Pdia3 Disulfide isomerase A3 Espermatogénesis 0,016 0,160 0,098 Ak1 Adenylate kinase 1 Motilidad 0,030 0,316 0,096 Sacy Soluble adenylate cyclase Capacitación 0,028 0,288 0,095 Cttn Cotractine Espermatogénesis 0,015 0,157 0,095 Pvrl2 Nectin 2 Espermatogénesis 0,021 0,231 0,090 Gpd2 Glycerol phospate deshydrogenase 2 Metabolismo 0,024 0,277 0,088 Boll Boule-like protein Espermatogénesis 0,013 0,152 0,086 Pcsk4 Proprotein convertase subtilisin/kexin type 4 Capacitación 0,020 0,229 0,085 Stx2 Sintaxin-2 Espermatogénesis 0,005 0,058 0,085 Pik3r3 Phosphatidil inositol 3 kinasa regulatory subunit 3 Reacción acrosómica 0,020 0,236 0,084 Dld Dihydrolipoamide dehydrogenase Capacitación 0,018 0,227 0,079 Etv5 ETS translocation variant 5 Espermatogénesis 0,016 0,202 0,077 Slc25a1 Solute carrier family 25 member 1 Motilidad 0,016 0,215 0,076 Idh1 Isocitrate dehydrogenase [NADP] cytoplasmic Metabolismo 0,015 0,212 0,072 Pebp1 Phosphatidylethanolamine-binding protein 1 Capacitación 0,036 0,516 0,070 Trpc3 Short transient receptor potential channel 3 Capacitación 0,024 0,344 0,069 Ddx4 Probable ATP-dependent RNA helicase Espermatogénesis 0,020 0,293 0,068 Pkm2 Pyruvate kinase isozymes M1/M2 Metabolismo 0,033 0,492 0,067 Tssk2 Testis-specific serine/threonine-protein kinase-2 Espermatogénesis 0,008 0,134 0,063 Cacna1h Voltage-dependent T-type calcium channel subunit alpha-1H Reacción acrosómica 0,017 0,279 0,062 Fkbp6 Peptidyl-prolyl cis-trans isomerase FKBP6 Espermatogénesis 0,013 0,213 0,062 Pgam2 Phosphoglycerate mutase 2 Metabolismo 0,016 0,259 0,061 Proteína Nombre de la proteína Proceso reproductivo dN dS ω Cacna1c Voltage-dependent L-type calcium channel subunit alpha-1C Capacitación 0,013 0,226 0,059 Csl Cytrate synthase-like gene Metabolismo 0,004 0,073 0,058 Mgea5 Beta-N-acetylhexosaminidase Interacción espermatozoide-óvulo 0,013 0,229 0,056 Hk1s Hexokinase-1, spermatogenic cell-specific Metabolismo 0,016 0,294 0,055 Eno1 Enolase 1 Metabolismo 0,016 0,285 0,054 Spata24 Espermatogénesis-associated protein 24 Espermatogénesis 0,014 0,271 0,053 Gas8 Growth arrest-specific protein 8 Motilidad 0,025 0,490 0,050 Cyct Cytochrome c, testis-specific Metabolismo 0,017 0,339 0,050 Sept4 Septin-4 Espermatogénesis 0,010 0,208 0,050 Nhe5 Sodium/hydrogen exchanger 5 Motilidad 0,010 0,205 0,047 Atp5b ATP synthetase beta subunit, mitochondrial Metabolismo 0,008 0,168 0,046 Pyk2 Protein-tyrosine kinase 2-beta Capacitación 0,002 0,046 0,043 Syt6 Synaptotagmin-6 Reacción acrosómica 0,010 0,259 0,037 Celf3 CUGBP Elav-like family member 3 Espermatogénesis 0,004 0,110 0,036 Prkcz Protein kinase C zeta type Reacción acrosómica 0,000 0,006 0,035 Trim36 E3 ubiquitin-protein ligase Trim36 Reacción acrosómica 0,007 0,202 0,035 Adcy8 Adenylate cyclase type 8 Capacitación 0,005 0,193 0,027 Chrna7 Neuronal acetylcholine receptor subunit alpha-7 Motilidad 0,004 0,153 0,025 Pfkfb4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 Metabolismo 0,005 0,189 0,025 Ift88 Intraflagellar transport protein 88 homolog Motilidad 0,005 0,189 0,025 Neurl1a Neuralized-like protein 1A Motilidad 0,002 0,094 0,025 Adcy2 Adenylate cyclase type 2 Capacitación 0,005 0,224 0,022 Rab14 Ras-related protein Rab-14 Capacitación 0,002 0,098 0,021 Spag6 Sperm associated antigen 6 Motilidad 0,005 0,234 0,021 Prkaca Sperm -specific protein kinase A catalytic subunit Capacitación 0,002 0,148 0,017 Dazla Deleted in azoospermia-like Espermatogénesis 0,002 0,148 0,012 Tssk6 Testis-specific serine/threonine-protein kinase-6 Espermatogénesis 0,002 0,149 0,011 Nhe1 Sodium/hydrogen exchanger 1 Motilidad 0,025 3,366 0,007 Glra1 Glycine receptor subunit alpha 1 Reacción acrosómica 0,002 0,355 0,005 Vcp Transitional endoplasmic reticulum ATPase Reacción acrosómica 0,001 0,165 0,003 Akt1 RAC-alpha serine/threonine-protein kinase Reacción acrosómica 0,032 1,155 0,003 Calm1 Calmodulin Capacitación 0,000 0,006 0,000 Tabla suplementaria 3. Análisis de selección positiva en proteínas del espermatozoide de ratón. Las proteínas bajo selección positiva se indican en negrita. La evidencia de selección positiva se detecta cuando el valor del LRT, que compara las probabilidades obtenidas de los modelos M8a (neutro) y M8a (selección), es mayor que el de un estadístico chi-cuadrado con un valor crítico de 5,41 en un nivel de significancia del 1%. Los aminoácidos bajo selección positiva con probabilidades posteriores > 0,95 (*) y > 0,99 (**) son mostrados. Proteína Nombre de la proteína Proceso reproductivo LRT M8vsM8a Aminoácidos bajo selección positiva

G6pd2 Glucose-6-phosphate 1-dehydrogenase 2 Metabolismo 104,84 231A**, 294R** Fscb Fibrous sheat CABYR-binding protein Espermatogénesis 85,52 105I*, 459S**, 551P**, 572F**, 857G**, 878M*, 978S** Smky Motilidad Kinase Y Motilidad 85,18 29K*, 34L**, 46K**, 60L**, 65C**, 69A**, 75I**, 91F**, 115S*, 140G*, 141L**, 156D*, 179H*, 186G**, 199T**, 222I**, 243N**, 246K*, 273R*, 276C**, 280T**, 287V**, 298A*, 330E**, 359E**, 372S*, 381P*, 390V**, 402S**, 407L**, 409E**, 410P**, 415P**, 461R**, 494Q** Chdh Choline dehydrogenase Motilidad 48,80 4V**, 12W**, 24Q** Adam24 A disintegrin and metallopeptidase domain 24 Interacción espermatozoide-óvulo 44,03 453H**, 620D**, 677N*, 720E**

Smok2a Motilidad kinase 2A Motilidad 40,73 7Q*, 36S*, 38K**, 98R*, 109A**, 174G*, 181T*, 224T*, 240M* Idh1 Isocitrate dehydrogenase [NADP] cytoplasmic Metabolismo 38,24 229K*

Ropn1 Ropporin-1 Motilidad 34,38 149N* Smok2b Motilidad kinase 2B Motilidad 33,33 89R*, 101A*, 173G*, 180T*, 204V**, 374S* Gapdhs Glyceraldehyde-3-phosphate dehydrogenase, Metabolismo 31,18 spermatogenic Pgk2 Phosphoglycerate kinase 2 Metabolismo 27,31 151Q**, 176T** Krt9 Keratin-9 Espermatogénesis 26,95 154A*, 168P*, 499H*, 532H*, 548G*, 572H*, 586G*, 587S*, 589G*, 591Y*, 592G*, 594G*, 595S* Fsip2 Fibrous sheath-interacting protein 2 Capacitación 24,44 1247R*, 2310T*, 2627N*, 2903P*, 3311R*, 3547I*, 5639S*, 5755T*, 5844R*, 5903E*, 6823Y* Crisp1 Cysteine-rich secretory protein 1 Interacción espermatozoide-óvulo 24,08 112K**, 125V*, 201K*, 208S*, 212E*

Atp1a4 Sodium/potassium-transporting ATPase subunit Motilidad 24,07 alpha-4 Afaf Acrosome formation- associated factor Reacción acrosómica 22,45 50A*, 123T** Adam18 A disintegrin and metallopeptidase domain 18 Interacción espermatozoide-óvulo 21,93 35H*, 145N*, 159T*, 327R*

Sp56 Zona pellucida sperm-binding protein 3 receptor Interacción espermatozoide-óvulo 21,48 7I*, 8E**, 228L**, 519A**

Prm1 Protamine 1 Espermatogénesis 21,40 46I** Adam32 A disintegrin and metallopeptidase domain 32 Interacción espermatozoide-óvulo 21,01 154I*, 342S*, 422R*, 427A*, 456E*

Car4 Carbonic anhydrase-4 Motilidad 19,31 97V** Nhe5 Sodium/hydrogen exchanger 5 Motilidad 19,26 Tekt4 Tektin-4 Motilidad 18,75 4T*, 145T*, 210S**, 262Q** Pdia3 Disulfide isomerase A3 Espermatogénesis 18,72 20R* Smcp Sperm mitochondria-associated cysteine-rich Motilidad 15,36 51P*, 69P*, 74Q*, 109T**, 130N* protein. Proteína Nombre de la proteína Proceso reproductivo LRT M8vsM8a Aminoácidos bajo selección positiva

CatsperG Cation channel sperm associated protein G Motilidad 13,77 323H* Plscr2 Phospholipid scramblase 2 Capacitación 13,69 177K (**) Tctex5 Dynein light chain Tctex-type 5 Motilidad 13,26 23T*, 79S** Slxl1 Putative novel protein similar to Xlr-related, Interacción espermatozoide-óvulo 12,56 34I**, 76E** meiosis regulated Xmr Pkm2 Pyruvate kinase isozymes M1/M2 Metabolismo 12,38 385L*, 388I**, 393T* Catsper3 Cation channel sperm associated protein 3 Motilidad 12,31 Tnp2 Transition protein-2 Espermatogénesis 11,98 Zan Zonadhesin Interacción espermatozoide-óvulo 11,97 104Q**, 692A**, 703Y*, 779Y*, 868T**, 878L**, 956V*, 959V**, 997S**, 1032E*, 1042V*, 1645V**, 1767M**, 2714A**, 2765D**, 2854T*, 2889M**, 3109T**, 3203*, 3214**, 3587T**, 3671P**, 3690S**, 3720D**, 3761V**, 3853K**, 3897H**, 3898N*, 4032G**, 4132T**, 4300G**, 4307S**, 4358T*, 4363N*, 4490E**, 4533D*, 4674I**, 5216A* Celf3 CUGBP Elav-like family member 3 Espermatogénesis 11,91 Ph20 Hyaluronidase PH-20 Interacción espermatozoide-óvulo 11,65 8H*, 97L*, 123R*

Tmem190 Transmembrane protein 190 Interacción espermatozoide-óvulo 10,44 117H*, 143V*, 144L*

CatSper2 Cation channel sperm associated protein 2 Motilidad 10,15 49A*, 490I** Spatc1 Speriolin Espermatogénesis 9,76 Rsph1 Radial spoke head 1 homolog Espermatogénesis 9,59 189T*, 238P*, 260L* Sptrx1 Thioredoxin domain-containing protein 2 Espermatogénesis 9,39 266S*, 272Q*, 323E*, 387G* Dlat Dihydrolipoyllysine-residue acetyltransferase Metabolismo 8,82 172D**

Acrv1 Acrosomal protein SP-10 Interacción espermatozoide-óvulo 8,45

Clgn Calmegin Interacción espermatozoide-óvulo 8,29 2R**, 291K*

Cacna1c Voltage-dependent L-type calcium channel Capacitación 7,23 subunit alpha-1C Spata16 Espermatogénesis-associated protein 16 Espermatogénesis 6,76 Zpbp2 Zona pellucida binding protein-2 Interacción espermatozoide-óvulo 5,94

Mgea5 Beta-N-acetylhexosaminidase Interacción espermatozoide-óvulo 5,78

Trpc3 Short transient receptor potential channel 3 Capacitación 5,47 Oxct2a Succinyl-CoA:3-ketoacid-coenzyme A transferase Metabolismo 5,12 2A, mitochondrial Zpbp1 Zona pellucida binding protein-1 Interacción espermatozoide-óvulo 5,04 Cabyr Calcium-binding tyrosine phosphorilation-regulated Capacitación 5,04 protein Prm2 Protamine 2 Espermatogénesis 4,45 Proteína Nombre de la proteína Proceso reproductivo LRT M8vsM8a Aminoácidos bajo selección positiva

Hyal5 Hyaluronidase-5 Interacción espermatozoide-óvulo 4,30 Gpd2 Glycerol phospate deshydrogenase 2 Metabolismo 4,30 Tnp1 Transition protein-1 Espermatogénesis 3,55 Taf7l Transcription initiation factor TFIID subunit 7-like Espermatogénesis 3,39

Plcd4 Phospholipase C delta 4 Reacción acrosómica 3,39 Mpi Mannose-6-phosphate isomerase Metabolismo 3,34 Stx2 Sintaxin-2 Espermatogénesis 3,28 Pde4d Phospodiesterase 4d Capacitación 3,25 Pla2g1b Phospholipase A2 Reacción acrosómica 2,93 Ak1 Adenylate kinase 1 Motilidad 2,64 Eno1 Enolase 1 Metabolismo 2,35 Rhpn1 Rhophilin-1 Motilidad 2,13 Ppp3r2 Calcineurin subunit B type 2 Capacitación 2,02 Adam1b A disintegrin and metallopeptidase domain 1b Interacción espermatozoide-óvulo 1,93 Atp8b3 ATPase class I type 8B member 3 Interacción espermatozoide-óvulo 1,80 Chrna7 Neuronal acetylcholine receptor subunit alpha-7 Motilidad 1,77 Boll Boule-like protein Espermatogénesis 1,74 Cacna1h Voltage-dependent T-type calcium channel subunit Reacción acrosómica 1,70 alpha-1H Tssk6 Testis-specific serine/threonine-protein kinase-6 Espermatogénesis 1,65 Bsg Basigin Interacción espermatozoide-óvulo 1,36 Sed1 Bimotif EGF Repeat and Discoidin-Domain Protein-1 Interacción espermatozoide-óvulo 1,02

Pvrl2 Nectin 2 Espermatogénesis 0,98 Hils1 Histone H1-like protein in spermatids 1 Espermatogénesis 0,90 Tekt3 Tektin-3 Motilidad 0,89 Ldhc L-lactate dehydrogenase C chain Metabolismo 0,80 Ptpn1 Tyrosine-protein phosphatase non-receptor type 1 Reacción acrosómica 0,73

Dbil5 Diazepam-binding inhibitor-like 5 Metabolismo 0,65 Cttn Cotractine Espermatogénesis 0,58 Dpep3 Dipeptidase 3 Espermatogénesis 0,56 Adcy2 Adenylate cyclase type 2 Capacitación 0,34 Dnmt3l DNA (cytosine-5)-methyltransferase 3-like Espermatogénesis 0,32 Acrbp Acrosin binding protein Reacción acrosómica 0,30 Tctex1 Dynein light chain Tctex-type 1 Motilidad 0,20 Tcte1 T-complex-associated testis-expressed protein 1 Interacción espermatozoide-óvulo 0,20 Sept4 Septin-4 Espermatogénesis 0,18 Pebp1 Phosphatidylethanolamine-binding protein 1 Capacitación 0,15 Gpi1 Glucose phosphate isomerase Metabolismo 0,07 Pgam2 Phosphoglycerate mutase 2 Metabolismo 0,07 Proteína Nombre de la proteína Proceso reproductivo LRT M8vsM8a Aminoácidos bajo selección positiva

Slc25a1 Solute carrier family 25 member 1 Motilidad 0,07 Ctsl1 Cysteine-specific cathepsin Interacción espermatozoide-óvulo 0,04 Aldoa1 Fructosa bisphosphate aldolase Metabolismo 0,01 Prkaca Sperm -specific protein kinase A catalytic subunit Capacitación 0,01

Akt1 RAC-alpha serine/threonine-protein kinase Reacción acrosómica 0,00 Tssk1 Testis-specific serine/threonine-protein kinase-1 Espermatogénesis 0,00 Pcsk4 Proprotein convertase subtilisin/kexin type 4 Capacitación 0,00 B4galt1 Beta-1,4 galactosyltransferase 1 Interacción espermatozoide-óvulo 0,00 Hk1s Hexokinase-1, spermatogenic cell-specific Metabolismo 0,00 Spesp1 Sperm equatorial segment protein 1 Interacción espermatozoide-óvulo 0,00 Calm1 Calmodulin Capacitación 0,00 Vdac3 Voltage-dependent-anion channel mitochondrial Espermatogénesis 0,00 Vcp Transitional endoplasmic reticulum ATPase Reacción acrosómica 0,00 Prkcz Protein kinase C zeta type Reacción acrosómica 0,00 Arsa Arysulfatase A Interacción espermatozoide-óvulo 0,00 Calca Calcitonin-related polypeptide, alpha Capacitación 0,00 Spaca3 Sperm acrosome membrane-associated protein 3 Interacción espermatozoide-óvulo 0,00 Enkur Enkurin Reacción acrosómica 0,00 Spata24 Espermatogénesis-associated protein 24 Espermatogénesis 0,00 Pik3c3 Phosphoinositide-3-kinase, class 3 Reacción acrosómica 0,00 Cox6b2 Cytochrome c oxidase subunit 6B2 Metabolismo 0,00 Crisp2 Cysteine-rich secretory protein 2 Interacción espermatozoide-óvulo 0,00 Itpr1 Inositol 1,4,5-trisphosphate receptor type 1 Reacción acrosómica 0,00 Dkkl1 Dickkofp-like 1 Interacción espermatozoide-óvulo 0,00 Ccna1 Cyclin A1 Espermatogénesis 0,00 Pdpk1 3-phosphoinositide dependent protein kinase 1 Reacción acrosómica 0,00 Atp5b ATP synthetase beta subunit, mitochondrial Metabolismo 0,00 Pfkfb4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase Metabolismo 0,00 4 Ift88 Intraflagellar transport protein 88 homolog Motilidad 0,00 Rab14 Ras-related protein Rab-14 Capacitación 0,00 Dazla Deleted in azoospermia-like Espermatogénesis 0,00 Glra1 Glycine receptor subunit alpha 1 Reacción acrosómica 0,00 Adcy8 Adenylate cyclase type 8 Capacitación 0,00 Cyct Cytochrome c, testis-specific Metabolismo 0,00 Tex101 Testis-expressed protein 101 Capacitación 0,00 Fkbp6 Peptidyl-prolyl cis-trans isomerase FKBP6 Espermatogénesis 0,00 Csl Cytrate synthase-like gene Metabolismo 0,00 Ace Angiotensin-converting enzyme Interacción espermatozoide-óvulo 0,00 Syt6 Synaptotagmin-6 Reacción acrosómica 0,00 Pik3r3 Phosphatidil inositol 3 kinasa regulatory subunit 3 Reacción acrosómica 0,00 Proteína Nombre de la proteína Proceso reproductivo LRT M8vsM8a Aminoácidos bajo selección positiva

Tssk2 Testis-specific serine/threonine-protein kinase-2 Espermatogénesis 0,00 Akap110 A- kinase anchor protein 3 Motilidad 0,00 Glut3 Solute carrier family 2, facilitated glucose transporter Metabolismo -0,01 member 3 Etv5 ETS translocation variant 5 Espermatogénesis -0,01 Akap82 A- kinase anchor protein 82 Motilidad -0,01 Sacy Soluble adenylate cyclase Capacitación -0,03 Gas8 Growth arrest-specific protein 8 Motilidad -0,05 Pyk2 Protein-tyrosine kinase 2-beta Capacitación -0,06 Dnah12 Dynein heavy chain 12 Motilidad -0,10 Ddx4 Probable ATP-dependent RNA helicase Espermatogénesis -0,87 Trim36 E3 ubiquitin-protein ligase Trim36 Reacción acrosómica -1,41 Odf4 Outer dense fiber protein-4 Motilidad -1,49 Izumo1 Izumo sperm-egg fusion protein 1 Interacción espermatozoide-óvulo -2,06 Crisp4 Cysteine-rich secretory protein 4 Interacción espermatozoide-óvulo -2,99 Neurl1a Neuralized-like protein 1A Motilidad -3,15 Adam3 A disintegrin and metallopeptidase domain 3 Interacción espermatozoide-óvulo -3,80 Syt8 Synaptotagmin-8 Reacción acrosómica -4,08 Dld Dihydrolipoamide dehydrogenase Capacitación -6,55 Adam2 A disintegrin and metallopeptidase domain 2 Interacción espermatozoide-óvulo -8,19 H1fnt Testis-specific H1 histone Espermatogénesis -9,84 Pkdrej Polycystic kidney disease and receptor for egg jelly- Interacción espermatozoide-óvulo -11,25 related protein Tekt2 Tektin-2 Espermatogénesis -15,23 Pmca4 Plasma membrane calcium/calmodulin-dependent Motilidad -22,61 calcium ATPase, isoform 4 Trpc2 Short transient receptor potential channel 2 Reacción acrosómica -23,05 Svs2 Semenogelin-1 Capacitación -24,43 Catsper4 Cation channel sperm associated protein 4 Motilidad -27,34 Catsper1 Cation channel sperm associated protein 1 Motilidad -27,89 Slo3 Potassium channel subfamily U member 1 Capacitación -28,12 Spag6 Sperm associated antigen 6 Motilidad -34,96 Adam1a A disintegrin and metallopeptidase domain 1a Interacción espermatozoide-óvulo -40,16 Smok4a Motilidad kinase 4A Motilidad -45,89 Cd46 CD46 antigen, complement regulatory protein Reacción acrosómica -56,32 Pdha2 Piruvate dehydrogenase A2 Metabolismo -65,57 Nhe1 Sodium/hydrogen exchanger 1 Motilidad -179,12 Nhe10 Sodium/hydrogen exchanger 10 Motilidad -190,45 Tabla suplementaria 4. Datos usados para los análisis de Catsper1.

Número de Masa Masa Masa relativa de Valor ω de Proporción de Especie amino ácidos de corporal testicular testículo Catsper1 histidinas Catsper1 Mus m. musculus 30,08 0,12 0,277 1,671 309 17,80 Mus m. castaneus 23,67 0,31 0,861 1,671 307 17,59 Mus m. bactrianus 16,36 0,14 0,506 1,467 313 16,61 Mus m. domesticus 19,50 0,10 0,328 1,671 307 17,59 Mus macedonicus 18,40 0,28 0,966 1,674 306 16,67 Mus spicilegus 15,14 0,41 1,612 1,674 306 16,67 Mus spretus 17,01 0,30 1,070 1,688 308 18,51 Mus famulus 27,40 0,05 0,134 1,628 304 17,76 Mus cokii 18,60 0,18 0,610 1,634 306 16,99 Mus caroli 7,95 0,11 0,700 1,633 313 18,21 Mus pahari 20,09 0,13 0,407 1,698 315 13,97 Mus minutoides 5,54 0,10 0,900 1,763 305 15,08 Mastomys natalensis 78,25 0,92 1,030 1,676 299 15,38 Apodemus sylvaticus 30,43 0,96 2,236 1,201 293 15,36 Lemniscomys barbarus 44,62 0,67 1,150 0,929 319 18,18 Rattus norvegicus 500,00 4,00 1,302 1,799 299 11,71 Tabla suplementaria 5, Tasas evolutivas específicas de PKDREJ en roedores, Los parámetros evolutivos de cada especie se calcularon desde la raíz de la filogenia a la respectiva rama terminal,

Familia Especie dN dS ω Muridae Mus spicilegus 0,0829 0,1654 0,5013 Mus spretus 0,0794 0,1786 0,4449 Mus macedonicus 0,0829 0,1654 0,5015 Mus cookii 0,0844 0,1617 0,5217 Mus musculus bactrianus 0,0818 0,1654 0,4944 Mus musculus domesticus 0,0806 0,1654 0,4873 Mus musculus musculus 0,0806 0,1654 0,4873 Mus musculus castaneus 0,0829 0,1680 0,4937 Mus pahari 0,0775 0,1661 0,4663 Mus famulus 0,0806 0,1641 0,4910 Apodemus sylvaticus 0,0627 0,1478 0,4242

Cricetidae Arvicola sapidus 0,1070 0,2850 0,3754 Arvicola terrestris 0,1050 0,2930 0,3584 Clethrionomys glareolus 0,1210 0,3010 0,4020 Chionomys nivalis 0,1120 0,2810 0,3986 Microtus arvalis 0,1190 0,2910 0,4089 Microtus cabrerae 0,1210 0,2910 0,4158 Pitimys duodecimostatus 0,1210 0,2910 0,4158 Pitimys lusitanicus 0,1220 0,2940 0,4150 Mesocricetus auratus 0,0990 0,2230 0,4439 Cricetulus griseus 0,1210 0,2320 0,5216 Phodopus sungorus 0,1080 0,2620 0,4122 Phodopus campbelli 0,1080 0,2620 0,4122 Phodopus roborovskii 0,1060 0,2880 0,3681 Tabla suplementaria 6. Masa corporal, masa testicular y masa testicular residual de primates. Los valores de masa testicular residual se transformaron a escala logarítmica porque no se ajustaban a una distribución normal. Las especies aparecen ordenadas por los valores logarítmicos de la masa testicular residual. Las referencias de los trabajos de donde se extrajeron los datos son citadas.

Masa Masa Masa Log-Masa Especie corporal testicular testicular testicular Referencia bibliográfica (g) (g) residual residual Macaca mulatta 10430 76.0 27.527 0.569 Kenagy y Trombulak (1986) Macaca nemestrina 9980 66.7 18.252 0.525 Kenagy y Trombulak (1986) Ateles geoffroyi 9800 64.2 15.792 0.514 Anderson y Dixson (2004) Pan panyscus 39100 135.2 85.136 0.456 Anderson y Dixson (2004) Pan troglodytes 44340 118.8 68.445 0.366 Kenagy y Trombulak (1986) Homo sapiens 63540 50.2 -1.221 -0.107 Kenagy y Trombulak (1986) Callithrix jacchus 320 1.3 -46.611 -0.24 Kenagy y Trombulak (1986) Pongo pygmaeus 74640 36.3 -15.738 -0.292 Kenagy y Trombulak (1986) Saguinus labiatus 501 1.5 -46.441 -0.307 Kenagy y Trombulak (1986) Lemur catta 27000 17.8 -31.593 -0.322 Anderson y Dixson (2004) Erythrocebus patas 13000 7.2 -41.415 -0.515 Anderson y Dixson (2004) Gorilla gorilla 134000 23.2 -32.133 -0.648 Kenagy y Trombulak (1986)

Tabla suplementaria 7. Datos de MS para las proteínas identificadas en la especie Mus musculus.

Réplica 1 Réplica 2 Réplica 3 Datos combinados

114326522 Cd55 complement decay-accelerating factor, GPI-anchored precursor 1 9,7 3 5 0,9995 7,7 2 2 1 19,5 6 6 1 14,1 5 14 0,000434 disintegrin and metalloproteinase domain-containing protein 1b 114842412 Adam1b precursor 1 5,5 4 4 1 4,6 3 5 1 10,8 7 7 1 9,3 6 17 0,000265 11496255 LOC100048331 dnaJ homolog subfamily A member 4 1 8,6 3 4 0,9994 5,8 2 4 1 25,9 7 7 1 25,9 7 16 0,0003 115334671 ALDH9A1 4-trimethylaminobutyraldehyde dehydrogenase precursor 1 6,4 3 5 1 10,8 5 6 1 34 13 13 1 35,9 14 25 0,000381 116014342 bsg basigin isoform 2 precursor 1 17,6 5 31 1 24,2 4 23 1 17,9 6 6 1 27,8 7 95 0,00347664 116089322 lonp1 lon protease homolog, mitochondrial precursor 1 13,1 10 10 1 4,8 4 7 1 16,4 11 11 1 21,5 16 35 0,000338 116805338 3110056O03Rik signal peptide peptidase-like 2B precursor 1 7,6 3 3 - 0 0 0 1 10,6 4 4 1 10,6 4 6 0,000152 117606270 NME2 nucleoside diphosphate kinase B 1 25 3 6 - 0 0 0 0,9992 40,1 5 5 0,9987 40,1 5 2 0,00011 117935034 1700061G19Rik uncharacterized protein LOC78625 1 8,2 5 7 1 6,5 4 5 1 8,2 5 5 1 10,2 6 18 0,000252 117938289 TSKS testis-specific serine kinase substrate isoform 1 1 7,2 3 4 - 0 0 0 1 18,3 7 7 1 16,2 6 15 0,000199 117956379 tekt1 tektin-1 1 34,4 13 23 1 11,7 4 5 1 34,2 13 13 1 37,8 15 60 0,0010279 118026915 EFCAB5 EF-hand calcium-binding domain-containing protein 5 1 2,4 3 4 - 0 0 0 1 4,3 5 5 1 4,3 5 11 0,0000697 118150641 ropn1l ropporin-1-like protein 1 35,3 7 8 1 16,5 3 4 1 35,8 8 8 1 35,8 8 25 0,000828 118403322 acad8 isobutyryl-CoA dehydrogenase, mitochondrial precursor 1 11,4 4 4 1 10,2 4 4 1 24,7 10 10 1 30,3 12 22 0,000362 succinyl-CoA:3-ketoacid coenzyme A transferase 2A, 11968160 Oxct2a mitochondrial precursor 1 69,4 26 249 1 45 16 153 1 75,6 28 28 1 76 30 768 0,00775306 119709839 Adam6a a disintegrin and metalloprotease domain 6 1 10,1 6 12 1 11,3 7 14 1 11,1 7 7 1 20,2 13 35 0,00044 120300928 KCNU1 potassium channel subfamily U member 1 1 3,8 4 4 - 0 0 0 - 1 1 1 1 3,8 4 5 0,0000612 120407035 Spam1 hyaluronidase PH-20 precursor 1 15,4 7 10 1 11,5 5 7 1 30,7 14 14 1 28,5 13 51 0,000875 120587011 SYNGR4 synaptogyrin-4 1 15 3 12 1 15 3 12 1 15 3 3 1 15 3 40 0,00325349 12331398 SCAMP2 secretory carrier-associated membrane protein 2 1 9,7 2 8 1 9,7 2 10 1 9,7 2 2 1 12,2 4 28 0,00276046 124001554 CATSPER2 cation channel sperm-associated protein 2 1 6,6 4 5 0,9994 3,7 2 2 1 8 4 4 1 7,5 4 10 0,000251 124244035 PPEF1 serine/threonine-protein phosphatase with EF-hands 1 1 7,4 4 4 - 0 0 0 1 6,9 4 4 1 8,9 5 6 0,0000908 124301182 9230107M04Rik binder of sperm protein homolog 2 precursor 1 22,9 3 3 - 6,9 1 1 - 0 0 0 1 22,9 3 4 0,0003 124339838 Hspa1l heat shock 70 kDa protein 1-like 1 37,1 18 26 1 25,3 12 15 1 43,4 21 21 1 43,2 22 94 0,00111864 124358957 TTC25 tetratricopeptide repeat protein 25 1 6,7 4 5 1 5,3 3 3 1 17,5 8 8 1 21,2 10 35 0,000432 124486773 Dnahc2 dynein heavy chain 2, axonemal 1 2,1 8 8 - 0 0 0 1 3,3 12 12 1 3,7 14 18 0,0000372 Réplica 1 Réplica 2 Réplica 3 Datos combinados

12963571 Ndufa7 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 1 27,4 3 3 - 8 1 1 1 28,3 4 4 1 28,3 4 9 0,000432 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12963633 Ndufa13 13 1 48,6 7 17 1 38,2 5 9 1 48,6 7 7 1 48,6 7 40 0,00188275 131889222 SDR39U1 epimerase family protein SDR39U1 1 19,5 6 10 1 11,9 4 9 1 19,1 5 5 1 19,5 6 29 0,0010301 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13195624 NDUFA10 10, mitochondrial precursor 1 35,8 9 14 1 12,1 4 5 1 56,6 16 16 1 56,6 15 51 0,0011763 13195690 Gm5928 ubiquitin-40S ribosomal protein S27a precursor 1 44,6 3 18 1 56,4 4 15 1 44,6 3 3 1 56,4 4 67 0,00315567 13249349 RANBP17 ran-binding protein 17 1 7,3 8 12 1 7,2 7 16 1 22,7 20 20 1 23,5 21 54 0,000506 CUB and zona pellucida-like domain-containing protein 1 133778951 CUZD1 precursor 1 5,9 3 5 1 8,6 5 12 1 25 11 11 1 23,4 10 45 0,000961 13384638 Rsph1 radial spoke head 1 homolog 1 35,2 8 18 1 27,9 6 15 1 48,2 11 11 1 48,2 11 71 0,00268621 13384778 pgls 6-phosphogluconolactonase 1 21 4 4 0,9989 9,7 2 2 1 19,1 4 4 1 33,5 7 12 0,000432 13384810 ACYP1 acylphosphatase-1 1 55,6 5 5 - 13,1 1 1 1 43,4 4 4 1 55,6 5 8 0,000484 13385006 cyc1 cytochrome c1, heme protein, mitochondrial 1 28 6 17 1 28 6 18 1 35,7 8 8 1 40,3 8 70 0,00275102

13385012 Gm9803 mitochondrial import inner membrane translocase subunit TIM16 1 52,8 4 4 - 10,4 1 1 1 31,2 3 3 1 52,8 4 9 0,000475

13385054 NDUFB3 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 1 33,7 3 4 - 10,6 1 1 0,9996 17,3 2 2 1 33,7 3 9 0,000608 13385124 ribC1 RIB43A-like with coiled-coils protein 1 1 16,4 5 7 1 10 3 6 1 20,1 7 7 1 18,2 6 25 0,000479 13385168 uqcrfs1 cytochrome b-c1 complex subunit Rieske, mitochondrial 1 42,7 8 30 1 29,9 5 13 1 54,7 11 11 1 59,5 12 88 0,00299653 13385180 Spesp1 sperm equatorial segment protein 1 precursor 1 50,1 13 83 1 42,6 11 79 1 55,6 16 16 1 54,6 15 315 0,0080014 13385188 KLHL10 kelch-like protein 10 1 9,5 5 5 1 7,7 4 7 1 11,2 6 6 1 11,2 6 20 0,000244 13385274 LOC100045163 phospholipase B-like 1 precursor 1 9,6 3 5 1 14,2 6 10 1 23,1 9 9 1 23,5 9 28 0,000538

13385322 Ndufb7 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 1 58,4 6 17 1 58,4 6 14 1 70,8 8 8 1 70,8 8 42 0,00187314 13385328 FANK1 fibronectin type 3 and ankyrin repeat domains 1 protein 1 17,2 5 5 1 8,7 3 3 1 17,7 5 5 1 22,7 7 14 0,00028

13385492 ndufa6 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 1 45,8 5 8 1 19,1 2 3 1 45,8 5 5 1 45,8 5 19 0,00112088 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, 13385558 Ndufb8 mitochondrial precursor 1 37,6 5 13 1 37,6 5 11 1 46,2 6 6 1 46,2 6 37 0,0014404 13385700 1700028P14Rik uncharacterized protein C9orf135 homolog 1 25,9 5 5 - 0 0 0 1 41,7 8 8 1 37,3 7 11 0,000268 13385716 1700019N19Rik uncharacterized protein C10orf82 homolog 1 25,3 5 9 - 3,8 1 4 1 40,5 8 8 1 40,5 8 30 0,00145685 13385726 Gm2962 cytochrome b-c1 complex subunit 7 1 27,9 4 8 1 18,9 3 5 1 34,2 5 5 1 27,9 4 20 0,00104433 13385730 GLIPR1L2 GLIPR1-like protein 2 1 6 2 6 0,9993 6 2 8 1 19,3 4 4 1 22,3 5 20 0,000743 13385762 Lrrc18 leucine-rich repeat-containing protein 18 isoform 1 1 16,1 3 8 - 4,3 1 2 - 4,3 1 1 1 16,1 3 13 0,000336 13385766 Ubxn11 UBX domain-containing protein 11 1 9,3 3 4 1 14 5 13 1 22,9 8 8 1 22,9 8 29 0,000523 13385800 Tceb2 transcription elongation factor B polypeptide 2 1 42,4 4 4 - 7,6 1 2 1 42,4 4 4 1 50 5 11 0,000754 13385842 4921517D21Rik uncharacterized protein LOC67722 1 4,6 3 3 - 0 0 0 1 7,5 6 6 1 5,4 4 8 0,0000788 13385942 CS citrate synthase, mitochondrial precursor 1 39 13 71 1 30,6 10 17 1 42,2 14 14 1 42,2 14 216 0,00419333 13385998 Trap1 heat shock protein 75 kDa, mitochondrial precursor 1 9,2 5 5 1 7,5 4 4 1 12,6 8 8 1 18,3 11 17 0,000185 13386040 atp5s ATP synthase subunit s, mitochondrial precursor 1 15 3 5 0,999 9,5 2 2 1 27 5 5 1 27 5 21 0,000775 13386062 APOOL apolipoprotein O-like precursor 1 24,9 5 8 - 0 0 0 1 41,1 10 10 1 32,5 7 14 0,000363 13386070 OTUB2 ubiquitin thioesterase OTUB2 isoform 2 1 13,2 3 3 - 4,3 1 1 0,9998 9,8 2 2 1 13,2 3 6 0,000196

13386100 NDUFA5 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 1 45,7 6 9 1 28,4 4 4 1 45,7 6 6 1 45,7 6 21 0,00148638 13386110 Efcab2 EF-hand calcium-binding domain-containing protein 2 1 24,4 3 3 0,9986 12,2 2 2 1 14 2 2 1 31,1 4 7 0,000434 13386316 Actrt2 actin-related protein T2 1 7,7 3 3 - 0 0 0 1 16,4 6 6 1 11,4 4 9 0,000206 134031980 Irgc1 interferon-inducible GTPase 5 1 45,6 16 66 1 27,4 10 40 1 43,8 15 15 1 47,5 17 193 0,003178 134031994 psmA2 proteasome subunit alpha type-2 1 29,1 5 5 0,999 12 2 2 1 29,1 6 6 1 29,1 6 13 0,000599 134053905 Psmd11 26S proteasome non-ATPase regulatory subunit 11 1 15,9 6 6 0,9992 4,5 2 3 1 21,8 8 8 1 21,8 9 16 0,00028 134053952 wdr16 WD repeat-containing protein 16 1 18,2 10 12 1 6,3 3 4 1 46,6 24 24 1 48,2 25 64 0,000844 134053957 SLC44A5 choline transporter-like protein 5 1 15,2 9 10 1 6,9 3 4 1 18,2 10 10 1 21,5 12 28 0,000484 13507710 Ropn1 ropporin-1 1 60,8 8 48 1 39,6 6 31 1 74,1 10 10 1 74,1 10 135 0,00389016 139948802 Uchl3 ubiquitin carboxyl-terminal hydrolase isozyme L3 1 37,8 6 7 0,9986 14,3 2 2 1 38,3 7 7 1 38,3 7 20 0,000756 14030781 TSSK6 testis-specific serine/threonine-protein kinase 6 1 18,7 5 7 0,9994 8,8 2 3 1 22 6 6 1 22 6 16 0,000442 Réplica 1 Réplica 2 Réplica 3 Datos combinados

145587094 9430016H08Rik uncharacterized protein C2orf47 homolog, mitochondrial precursor 1 15,1 4 7 - 3,8 1 2 1 13,1 4 4 1 15,1 4 12 0,000311 145966885 SPACA5 sperm acrosome-associated protein 5 precursor 1 13,1 3 4 - 6,2 1 1 1 13,1 3 3 1 13,1 3 9 0,000562 146262012 DDI1 protein DDI1 homolog 1 1 10,8 3 3 0,9995 7,6 2 6 0,9999 7,6 2 2 1 10,8 3 12 0,00038 148225693 TEKT2 tektin-2 1 48,1 16 37 1 35,8 12 18 1 58,6 22 22 1 58,6 22 117 0,00174858

148230380 Pebp4 phosphatidylethanolamine-binding protein 4 isoform a precursor 1 24,8 5 7 1 16,9 3 7 1 31,4 7 7 1 28,5 6 28 0,00110591 149251053 Gm5436 PREDICTED: ATP synthase subunit O, mitochondrial-like 1 61,5 10 30 1 46,5 7 19 1 61,5 10 10 1 61,5 10 90 0,00278754 PREDICTED: phosphate carrier protein, mitochondrial-like 149258501 SLC25A3 isoform 1 1 31,1 10 19 - 3,4 1 3 1 30,8 10 10 1 31,1 10 47 0,00112964 149258833 Gm16486 PREDICTED: uncharacterized protein KIAA1683 1 11,7 11 21 1 4,4 5 8 1 17 17 17 1 17 17 59 0,000508 PREDICTED: NADH dehydrogenase [ubiquinone] 1 beta 149266669 Gm3244 subcomplex subunit 4-like 1 46,5 5 8 1 46,5 5 8 1 46,5 5 5 1 46,5 5 21 0,00159484 PREDICTED: glyceraldehyde-3-phosphate dehydrogenase-like 149273202 Gm4335 isoform 2 1 24,9 7 8 1 23,4 6 8 1 55 13 13 1 52,6 12 62 0,00152 149944431 TEKT5 tektin-5 1 42,2 16 30 1 15,4 5 5 1 55,2 20 20 1 57,1 21 92 0,00166709 152963553 1190002A17Rik adenylate kinase 8 1 21,9 9 13 1 11,9 5 12 1 35,9 16 16 1 41,3 19 49 0,000697 153791341 Rsph3b radial spoke head protein 3 homolog B 1 19,5 8 29 1 14,9 5 15 1 17,5 7 7 1 25,2 9 74 0,00172531 153791370 odf3 outer dense fiber protein 3 1 44,9 9 31 1 23,6 5 21 1 54,3 12 12 1 59,1 12 99 0,00290354 153792273 LOC100046510 dynein heavy chain 8, axonemal 1 12,3 50 78 1 1,1 5 10 1 19,9 81 81 1 20 79 198 0,000421 154091013 CCDC135 coiled-coil domain-containing protein lobo homolog 1 8,7 6 6 1 5,3 4 6 1 8,6 6 6 1 13,4 11 22 0,00022 154146209 Usp7 ubiquitin carboxyl-terminal hydrolase 7 1 9,6 9 9 1 5,1 5 5 1 6,3 6 6 1 13,3 13 24 0,000193 154426268 Acot10 acyl-coenzyme A thioesterase 10, mitochondrial precursor 1 39,9 17 5 1 18,7 8 18 1 29,2 13 13 1 39 18 12 0,000251 156255157 Acad10 acyl-CoA dehydrogenase family member 10 1 5,5 5 5 0,9991 1,7 2 2 1 7 6 6 1 8,4 7 13 0,000105 157057142 Abca14 ATP-binding cassette, sub-family A (ABC1), member 14 1 2,7 4 4 - 0 0 0 - 0,8 1 1 1 2,1 3 4 0,0000262 157057150 Abca17 ATP-binding cassette, sub-family A (ABC1), member 17 1 3,4 5 5 - 0 0 0 1 3,8 5 5 1 3,9 5 7 0,0000536 157785665 dtymk thymidylate kinase isoform 1 1 16 3 3 - 0 0 0 1 29,7 5 5 1 29,7 6 9 0,000276 157951596 Car2 carbonic anhydrase 2 1 24,2 5 5 1 44,6 7 8 1 62,3 11 11 1 62,3 11 35 0,00139332 157951639 4930404N11Rik uncharacterized protein C19orf71 homolog 1 40,3 6 13 1 31,1 5 13 1 33 6 6 1 43,7 7 45 0,00189041 157951659 CRISP1 cysteine-rich secretory protein 1 precursor 1 12,3 3 3 0,9986 8,6 2 2 1 23 5 5 1 27,5 6 17 0,000613 157951670 CAPZA3 F-actin-capping protein subunit alpha-3 1 30,8 9 15 1 12,7 4 8 1 23,4 6 6 1 36,1 9 31 0,000842 157951743 H2-Ke6 estradiol 17-beta-dehydrogenase 8 1 49,8 9 15 1 63,3 10 18 1 68 11 11 1 68 11 54 0,0019918 cation channel sperm-associated protein subunit gamma 2 157951745 Catsperg2 precursor 1 10,7 10 13 1 6,2 6 7 1 7,7 7 7 1 10,9 11 27 0,000284 158187507 Fam71a uncharacterized protein LOC619288 1 9,6 5 12 1 5,5 3 6 1 20,2 9 9 1 20,2 9 35 0,000577 158341656 ODF1 outer dense fiber protein 1 1 22,6 5 26 1 30,2 5 19 1 54 10 10 1 54 10 105 0,00285628 158853992 Gm5506 alpha-enolase 1 50,7 16 64 1 49,1 14 65 1 52,5 16 16 1 54,1 17 318 0,00505518 160333212 Canx calnexin precursor 1 8,3 4 4 0,9987 4,2 2 2 1 20,1 10 10 1 20 9 22 0,000496 160333353 trim69 E3 ubiquitin-protein ligase TRIM69 1 12,6 5 5 0,9989 4,2 2 2 1 29,6 12 12 1 23,6 9 19 0,000303 160333553 rpl12 60S ribosomal protein L12 1 33,9 4 4 1 49,1 5 6 1 54,5 6 6 1 54,5 6 21 0,000858 160358791 AKAP3 A-kinase anchor protein 3 1 36,9 24 121 1 12,6 9 44 1 52,4 35 35 1 50,6 33 385 0,00316404 succinyl-CoA:3-ketoacid coenzyme A transferase 2B, 160358827 LOC100046138 mitochondrial precursor 1 69,4 26 245 - 0 0 0 1 75,6 28 28 1 76 30 762 0,00771333 160420308 Hist1h2bp histone H2B type 1-P 1 27,8 3 8 - 0 0 0 - 0 0 0 1 27,8 3 11 0,000482 161016820 DNPEP aspartyl aminopeptidase isoform b 1 35,5 12 31 1 20,1 7 27 1 34,9 12 12 1 35,5 12 96 0,00207052 161016824 LOC100045796 cysteine desulfurase, mitochondrial 1 17,4 6 7 1 11,5 4 10 1 27,7 10 10 1 29,4 11 30 0,000491 161484642 LOC100044663 apolipoprotein O isoform 1 1 41,5 5 16 0,9994 15,9 2 9 1 47,6 6 6 1 41,5 5 39 0,00201878 162138915 cpt2 carnitine O-palmitoyltransferase 2, mitochondrial precursor 1 26 13 15 1 11,1 5 8 1 33,4 15 15 1 36,2 17 46 0,000661 162139827 ME1 NADP-dependent malic enzyme isoform 1 1 23 9 15 0,9988 2,9 2 2 1 37,4 15 15 1 38,6 16 41 0,000676 162287165 Cpt1b carnitine O-palmitoyltransferase 1, muscle isoform 1 22,5 15 16 1 4,9 3 4 1 16,5 13 13 1 22,2 15 29 0,000392 162287182 SPAG17 sperm-associated antigen 17 1 1,9 3 3 0,9987 0,7 2 3 1 4,7 8 8 1 5,8 11 17 0,0000819 162461907 HSPA9 stress-70 protein, mitochondrial 1 35,8 18 43 1 33 17 38 1 42,7 22 22 1 42,3 21 167 0,00172423 163838641 Atp5c1 ATP synthase subunit gamma, mitochondrial isoform a 1 26,3 7 17 1 16,8 4 11 1 47,1 10 10 1 43,1 9 59 0,0018635 164519120 CCDC42 coiled-coil domain-containing protein 42A 1 11,7 3 3 0,999 7,9 2 2 1 15,8 4 4 1 24,7 6 10 0,000235 164565394 DNAJC11 dnaJ homolog subfamily C member 11 1 24,7 12 20 0,9999 5,9 3 4 1 25,9 12 12 1 30,4 14 41 0,000574 164607162 Dnalc1 dynein light chain 1, axonemal 1 17,4 3 6 1 18,9 3 3 1 29,5 5 5 1 29,5 5 19 0,000684 165932339 cutC copper homeostasis protein cutC homolog isoform 2 1 24 4 6 1 17,2 3 6 1 36,3 6 6 1 36,3 6 25 0,00069 165932389 NUP155 nuclear pore complex protein Nup155 1 8,2 9 13 1 4,5 4 5 1 9,3 9 9 1 10,4 11 28 0,000272 166851828 Atp5d ATP synthase subunit delta, mitochondrial precursor 1 13,7 2 5 0,9989 8,3 1 5 1 13,7 2 2 1 13,7 2 17 0,0012504 167000069 TXNDC3 thioredoxin domain-containing protein 3 isoform 1 1 16 10 22 1 9,9 6 16 1 23,7 14 14 1 23,7 14 64 0,00109976 16716343 Gm16399 cytochrome c oxidase subunit 6C 1 46,1 5 20 1 46,1 5 15 1 47,4 6 6 1 47,4 6 57 0,00555615 167234369 NHEDC1 sodium/hydrogen exchanger 9B1 1 14,2 7 9 1 6,4 4 4 1 16,3 8 8 1 15,9 9 25 0,000587 167716840 COX2 cytochrome c oxidase subunit II [Mus musculus musculus] 1 27,8 5 36 1 11,5 2 14 - 0 0 0 0 0 5 0 - 168229152 Gm13570 brain protein 44-like protein 2 1 63 5 17 0,9999 26,1 3 9 1 31,9 3 3 1 63 5 46 0,00351489

170014720 BDH1 D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor 1 33,5 9 30 1 19,2 5 19 1 43,4 11 11 1 45,5 12 86 0,00172523

170763467 Timm44 mitochondrial import inner membrane translocase subunit TIM44 1 27,9 11 17 1 13,7 5 5 1 38,3 15 15 1 36,1 16 39 0,000609 171543846 GPX5 epididymal secretory glutathione peroxidase precursor 1 25,3 5 7 - 6,3 1 1 1 42,5 8 8 1 42,5 8 20 0,000728 18079339 ACO2 aconitate hydratase, mitochondrial precursor 1 48,5 28 112 1 31,9 19 107 1 52,3 30 30 1 52,3 30 495 0,00540919 Réplica 1 Réplica 2 Réplica 3 Datos combinados

21312012 Ndufa8 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 1 43,6 6 14 1 23,8 3 6 1 59,3 7 7 1 64 8 37 0,00205499 21312276 IMMP1L mitochondrial inner membrane protease subunit 1 1 31,3 4 5 0,9989 11,4 2 3 1 31,3 4 4 1 31,3 4 13 0,00062 21312546 Fam162a protein FAM162A 1 18,7 3 7 - 7,1 1 4 1 31,6 5 5 1 27,1 4 21 0,00139619 21312594 Gm3982 mitochondrial pyruvate carrier 2 1 51,2 5 28 - 6,3 1 10 1 51,2 5 5 1 51,2 5 61 0,005065 21312734 sept12 septin-12 isoform 1 1 17,3 4 4 0,9983 7,9 2 2 1 27,3 7 7 1 27,3 7 17 0,000467 21312794 Pfn3 profilin-3 1 47,4 6 8 1 35,8 4 4 1 48,2 6 6 1 54,7 7 22 0,0010318 21312834 TSSK4 testis-specific serine/threonine-protein kinase 4 isoform 1 1 35,4 11 19 1 14,3 4 5 1 36,9 10 10 1 45,1 13 41 0,000921 21312844 lrrc48 leucine-rich repeat-containing protein 48 1 11,9 5 5 0,9994 5,4 2 2 1 21,8 9 9 1 24,1 10 17 0,000314 21312846 SPACA1 sperm acrosome membrane-associated protein 1 precursor 1 38,4 7 69 1 30,2 6 86 1 30,2 6 6 1 38,4 7 294 0,01577152 21312922 CCDC39 coiled-coil domain-containing protein 39 1 10,1 8 10 1 8 6 6 1 13,4 11 11 1 12,5 10 32 0,000256 NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, 21312950 NDUFS7 mitochondrial precursor 1 21,4 5 15 1 18,3 4 13 1 21,9 6 6 1 32,1 7 50 0,00197104 21312974 RSPH9 radial spoke head protein 9 homolog 1 48,6 11 16 1 35,5 8 15 1 58,7 13 13 1 58,7 13 77 0,00207699 21312994 Slc25a11 mitochondrial 2-oxoglutarate/malate carrier protein 1 23,2 6 6 - 3,2 1 1 1 9,6 3 3 1 23,2 6 10 0,000226 21313012 RAB4B ras-related protein Rab-4B 1 28,2 5 7 0,9995 11,3 2 2 1 28,2 5 5 1 28,2 5 17 0,00048 21313146 ENKUR enkurin 1 22 5 5 - 0 0 0 1 43,1 11 11 1 43,1 11 35 0,000985 21313338 4933411K16Rik uncharacterized protein C10orf62 homolog 1 10,5 3 4 - 0 0 0 1 24 6 6 1 24 6 21 0,000905 dihydrolipoyllysine-residue succinyltransferase component of 2- 21313536 DLST oxoglutarate dehydrogenase complex, mitochondrial 1 20,9 7 19 1 10,1 3 13 1 38,8 10 10 1 38,8 10 57 0,00123024 21313544 BCS1L mitochondrial chaperone BCS1 1 16,7 5 5 0,9996 6,7 2 2 1 29,4 9 9 1 27,5 9 17 0,000361 coiled-coil-helix-coiled-coil-helix domain-containing protein 3, 21313618 CHCHD3 mitochondrial precursor 1 32,2 9 14 1 19,8 6 9 1 28,6 7 7 1 32,2 9 36 0,00107943 21313679 LOC674469 ATP synthase subunit d, mitochondrial 1 52,8 7 17 1 32,3 4 10 1 54 9 9 1 54 9 50 0,00267337 21313683 DPEP3 dipeptidase 3 precursor 1 29,8 10 33 1 22,9 8 33 1 32,7 12 12 1 31,2 11 120 0,00255772 21314832 UGP2 UTP--glucose-1-phosphate uridylyltransferase 1 7,7 3 3 - 0 0 0 - 0 0 0 1 7,7 3 3 0,0000464 21361209 Hist1h4c histone H4 1 43,7 5 6 0,9993 19,4 2 2 1 50,5 5 5 1 50,5 5 15 0,000877 214010170 LOC100046120 clusterin precursor 1 21,2 9 50 1 13,2 5 33 1 31,7 13 13 1 29,7 12 160 0,00377218 21450129 ACAT1 acetyl-CoA acetyltransferase, mitochondrial precursor 1 37,5 10 25 1 24,5 7 18 1 38,9 11 11 1 46 12 84 0,00188672 21450187 CECR5 cat eye syndrome critical region protein 5 homolog precursor 1 23,9 8 10 1 16,9 6 9 1 30,3 8 8 1 28,9 9 33 0,000818 21450625 EIF4A1 eukaryotic initiation factor 4A-I isoform 1 1 9,6 3 3 - 0 0 0 - 0 0 0 1 14 4 7 0,000151 21536248 GSTT3 glutathione S-transferase, theta 3 1 22,8 4 4 0,9993 11,6 2 2 1 17 3 3 1 25,7 5 9 0,000376 21539585 UQCRQ cytochrome b-c1 complex subunit 8 1 26,8 2 4 1 28 3 11 1 28 3 3 1 37,8 4 22 0,00193988

21539587 NDUFA3 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 1 50 3 3 - 0 0 0 0,9987 21,4 2 2 0,999 21,4 2 4 0,000452 21539599 Uqcrh cytochrome b-c1 complex subunit 6, mitochondrial 1 37,1 2 6 0,9989 37,1 2 2 0,9997 20,2 1 1 1 37,1 2 11 0,000926 21553309 APOA1BP NAD(P)H-hydrate epimerase precursor 1 16,3 3 3 0,9985 12,1 2 2 1 30,9 7 7 1 30,9 7 18 0,000623 21699068 PITRM1 presequence protease, mitochondrial precursor 1 16,1 12 13 1 3,5 3 3 1 22,5 18 18 1 25,9 20 37 0,000334 Réplica 1 Réplica 2 Réplica 3 Datos combinados

22550094 PRKAR2A cAMP-dependent protein kinase type II-alpha regulatory subunit 1 41,8 13 35 1 27,6 8 23 1 48 15 15 1 49,5 15 148 0,00310684 225543319 LOC675857 transitional endoplasmic reticulum ATPase 1 36,5 21 25 1 27,2 15 27 1 53,8 34 34 1 54,7 34 119 0,00118146 225543354 Zmynd12 zinc finger, MYND domain containing 12 1 18,2 5 5 1 19,3 5 9 1 27,5 9 9 1 25,1 8 24 0,000568 225543576 gtl3 UPF0468 protein C16orf80 homolog 1 32,1 7 11 1 20,7 5 6 1 36,3 8 8 1 36,3 8 34 0,00143979 225735582 HK1 hexokinase-1 isoform HK1-sb 1 47,3 39 220 1 38,1 30 148 1 52,7 44 44 1 51,5 41 725 0,00457733 225735584 HK1 hexokinase-1 isoform HK1 1 46,9 38 189 - 0 0 0 - 54,7 44 44 1 51,3 40 632 0,00408614 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, 225903452 Gm9050 mitochondrial-like 1 27,3 2 4 0,9982 27,3 2 2 0,9996 27,3 2 2 1 27,3 2 12 0,000863 226246531 PGK2 phosphoglycerate kinase 2 1 38,4 13 60 1 23,3 6 26 1 59,5 17 17 1 59,5 17 179 0,00335947 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 226437665 NDUFA11 11 1 35 3 5 - 11,2 1 1 0,9996 23,8 2 2 1 35 3 10 0,000859 226442740 sept10 septin-10 isoform 1 1 15 4 5 - 2,8 1 3 1 19,2 6 6 1 23,7 7 19 0,000374 226442909 NIPSNAP3A nipsnap homolog 3A 1 26,5 5 19 1 31,8 6 25 1 31,8 6 6 1 31,8 6 70 0,00364281 226442916 spata18 mitochondria-eating protein 1 18,4 9 37 1 15,3 7 23 1 28,1 11 11 1 30 12 111 0,00239952 226442984 PTCHD3 patched domain-containing protein 3 1 16,3 11 17 1 12,7 9 11 1 17,4 12 12 1 18 12 47 0,00089 226443038 Glipr1l3 GLI pathogenesis-related 1 like 1 precursor 1 30,5 7 15 1 10,6 2 6 1 38,1 9 9 1 38,1 9 44 0,00174918 226530884 pcmt1 protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 13,3 3 3 - 0 0 0 1 14,7 3 3 1 18,2 4 8 0,000243 226530999 DNAJB3 dnaJ homolog subfamily B member 3 1 16,9 4 4 - 0 0 0 1 12,4 3 3 1 16,9 4 7 0,000229 226693361 CCT6B T-complex protein 1 subunit zeta-2 1 11,9 6 3 - 0 0 0 1 19,8 8 8 1 21,5 9 8 0,00012 226823367 Fh1 fumarate hydratase, mitochondrial precursor 1 32,5 9 13 1 10,7 4 7 1 26,8 9 9 1 34,7 10 37 0,000691 226958349 TPI1 triosephosphate isomerase 1 61,2 14 41 1 37,1 10 19 1 61,2 14 14 1 61,2 14 116 0,00263342 226958351 ATP1A4 sodium/potassium-transporting ATPase subunit alpha-4 1 38,9 34 76 1 30,4 24 42 1 42,3 38 38 1 41,6 37 198 0,00196211 227116345 psmb2 proteasome subunit beta type-2 1 21,9 4 5 1 16,4 3 4 1 21,9 4 4 1 21,9 4 17 0,000715 227330593 Dnaic1 dynein intermediate chain 1, axonemal 1 26,8 15 29 1 11,4 6 11 1 33,5 17 17 1 38,9 20 78 0,000993 227330625 MAN2B2 epididymis-specific alpha-mannosidase precursor 1 9,8 8 9 1 12,5 9 13 1 11,8 10 10 1 18,5 13 36 0,000529 227430395 Lrrc57 leucine-rich repeat-containing protein 57 isoform b 1 18,4 4 5 - 0 0 0 1 23,4 5 5 1 18,4 4 14 0,000437 227908775 RSPH6A radial spoke head protein 6 homolog A isoform 1 1 10,3 5 8 1 13,4 6 17 1 19,4 8 8 1 19,8 9 53 0,00139986 228480241 MTX2 metaxin-2 1 20,2 4 8 1 20,2 4 7 1 27,4 5 5 1 28,5 5 23 0,00107309 228480264 Spaca4 sperm acrosome membrane-associated protein 4 precursor 1 35,4 3 4 1 33,1 3 6 1 33,1 3 3 1 40,9 4 17 0,00144693 229577240 Zp3r zona pellucida sperm-binding protein 3 receptor precursor 1 11,2 5 13 1 9,7 4 13 1 17,6 8 8 1 15,9 7 58 0,00120879 229608935 NME7 nucleoside diphosphate kinase 7 isoform 1 1 17,7 6 9 1 9,4 3 3 1 33,9 11 11 1 35,9 12 28 0,000585 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial 229892316 NDUFS1 precursor 1 48,1 26 70 1 26,3 15 32 1 57,5 31 31 1 54,3 28 216 0,00230251 NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, 23346461 ndufs2 mitochondrial precursor 1 29,6 11 17 1 14,9 6 18 1 30,9 13 13 1 39,5 15 58 0,00125012 23346525 CD109 CD109 antigen precursor 1 5,5 5 6 0,999 2,1 2 3 1 11,4 13 13 1 12,3 13 29 0,000236 23346533 PHOSPHO1 phosphoethanolamine/phosphocholine phosphatase 1 20,6 6 6 1 23,6 6 13 1 27 7 7 1 27 8 30 0,000846 23346557 oplaH 5-oxoprolinase 1 12,3 11 11 0,999 1,6 2 3 1 13 13 13 1 16,4 16 27 0,000244 23346595 2410015M20Rik protein QIL1 precursor 1 41,2 3 4 - 0 0 0 1 41,2 3 3 1 41,2 3 8 0,000653 amyotrophic lateral sclerosis 2 (juvenile) chromosome region, 237649035 Als2cr11 candidate 11 1 6,8 3 5 0,9993 4,3 2 9 0,9993 3,6 2 2 1 6,8 3 16 0,000262 237874196 Dnahc7a dynein, axonemal, heavy chain 7B 1 4,6 17 21 1 0,8 3 6 1 11,3 38 38 1 10,9 38 21 0,0000464 238231384 PSMB6 proteasome subunit beta type-6 precursor 1 12,6 3 6 0,999 8,8 2 6 0,9998 8,8 2 2 1 12,6 3 14 0,000519 238814391 Cct7 T-complex protein 1 subunit eta 1 17,5 8 15 0,999 3,9 2 7 1 31,2 14 14 1 31,2 14 48 0,000672

239052655 EFCAB6 EF-hand calcium-binding domain-containing protein 6 isoform 1 1 16,8 10 16 1 8,4 5 11 1 28,6 19 19 1 27,7 18 71 0,000353 23943818 ccdc65 coiled-coil domain-containing protein 65 1 23,9 10 10 1 9,5 4 4 1 18,7 8 8 1 28 11 22 0,000338 23943838 slc25a1 solute carrier family 25, member 1 1 18 6 6 0,9999 9,3 3 3 1 13,2 4 4 1 15,8 5 13 0,000348 Réplica 1 Réplica 2 Réplica 3 Datos combinados

23956162 AGPAT2 1-acyl-sn-glycerol-3-phosphate acyltransferase beta precursor 1 6,8 2 3 0,9993 6,8 2 2 0,9995 6,8 2 2 1 6,8 2 7 0,00024 23956396 Erlin2 erlin-2 1 10,6 3 7 0,9993 7,1 2 3 1 40 11 11 1 37,1 10 27 0,000624 240120054 Akr7a5 aflatoxin B1 aldehyde reductase member 2 1 24 7 11 1 23,4 7 11 1 22,3 8 8 1 33,2 11 33 0,0010769 241666436 Ribc2 RIB43A-like with coiled-coils protein 2 1 13 4 5 0,9991 6,6 2 3 1 23,3 8 8 1 30 10 20 0,000384 241982800 1700080O16Rik uncharacterized protein LOC74279 1 15,8 3 4 0,999 12,3 2 7 1 9,5 3 3 1 21,5 5 15 0,00043 242247109 CYLC2 cylicin, basic protein of sperm head cytoskeleton 2 1 11,1 3 12 1 11,4 4 14 1 11,1 3 3 1 11,4 4 37 0,000692 242397497 1700012P22Rik uncharacterized protein C1orf158 homolog 1 41,8 7 9 1 16,3 3 3 1 45,4 8 8 1 45,4 8 27 0,00107324 244790045 SQRDL sulfide:quinone oxidoreductase, mitochondrial 1 27,3 11 14 1 13,6 6 10 1 34,7 14 14 1 38 15 42 0,000633 247270151 ftmt ferritin, mitochondrial precursor 1 13,9 3 6 1 13,9 3 7 1 24,9 4 4 1 24,9 4 18 0,000606 25072207 Dynlrb2 dynein light chain roadblock-type 2 1 60,4 4 6 0,9992 21,9 2 2 1 60,4 4 4 1 60,4 4 13 0,000923 25092662 vps25 vacuolar protein-sorting-associated protein 25 1 33,5 3 4 0,999 11,4 2 2 1 24,4 4 4 1 24,4 4 9 0,000393 251823978 Pcx pyruvate carboxylase, mitochondrial isoform 2 1 16,8 16 21 1 13,2 12 14 1 20,7 19 19 1 27,4 25 64 0,00045 253795525 CCDC147 coiled-coil domain containing 147 1 23,7 20 42 1 16,3 12 17 1 25,9 20 20 1 28,2 23 95 0,000746 253795534 FSCB fibrous sheath CABYR-binding protein 1 33,6 22 61 1 18,4 12 29 1 36,8 23 23 1 36,8 23 187 0,00332302 254281222 HTRA2 serine protease HTRA2, mitochondrial 1 25,1 8 9 1 11,6 4 6 1 37,1 11 11 1 37,3 11 29 0,000588 254540082 PSMB4 proteasome subunit beta type-4 precursor 1 33 5 6 1 25,4 4 6 1 42 6 6 1 42 6 23 0,000863 254553287 Tmem89 transmembrane protein 89 precursor 1 21,3 3 4 - 0 0 0 - 7,1 1 1 1 21,3 3 4 0,000404 254553303 DYDC1 DPY30 domain-containing protein 1 1 21,7 4 4 1 32,6 5 9 1 25,7 4 4 1 45,7 7 21 0,000881 254553344 PFKM 6-phosphofructokinase, muscle type 1 31 19 31 1 19,7 11 20 1 25,4 15 15 1 34,9 20 86 0,00103122 254553376 CCDC105 coiled-coil domain-containing protein 105 1 25,3 12 27 1 12,4 6 23 1 33,7 16 16 1 30,3 14 79 0,00115089 254553418 1700019O17Rik uncharacterized protein C2orf57 homolog 1 10,8 3 7 1 10,8 3 6 1 12,3 4 4 1 15,7 5 20 0,000709 254553422 EFHC1 EF-hand domain-containing protein 1 1 32,9 15 21 1 11,9 7 7 1 36,4 18 18 1 47,8 23 70 0,000832 254553434 IL4I1 L-amino-acid oxidase precursor 1 23 11 21 1 15,2 7 16 1 27,5 13 13 1 28,7 14 82 0,00151463 254553458 gpi1 glucose-6-phosphate isomerase 1 36,6 16 31 1 10,4 5 12 1 40,5 18 18 1 40,5 18 95 0,00185057 serine/threonine-protein phosphatase PGAM5, mitochondrial 254587960 pgam5 isoform 2 1 28,2 8 10 1 27,9 7 10 1 37,3 8 8 1 41,5 10 31 0,000775 254588114 Ttc39d tetratricopeptide repeat domain 39D 1 7,5 3 3 - 1,8 1 1 1 7,6 4 4 1 11,1 5 8 0,000131 254675102 RTDR1 rhabdoid tumor deletion region gene 1 isoform 1 1 32,3 9 13 1 14,1 4 5 1 32,3 9 9 1 34,6 10 30 0,000662 254675150 hspb9 heat shock protein beta-9 1 31,7 6 22 1 27,1 5 18 1 52,3 9 9 1 52,3 9 64 0,00224865

254675160 4921530D09Rik uncharacterized coiled-coil domain-containing protein KIAA1984 1 15,9 7 22 1 6,9 3 10 1 28,3 13 13 1 31,1 15 59 0,000791 254675162 NUP210L nuclear pore membrane glycoprotein 210-like precursor 1 7,1 12 16 1 5,4 9 9 1 11,3 17 17 1 11,9 19 43 0,000231 254675166 ak7 adenylate kinase 7 1 22,4 12 17 1 12,9 7 15 1 25,9 14 14 1 29,2 16 59 0,000794 254692831 PSMA8 proteasome subunit alpha type-7-like 1 27,6 5 9 1 27,6 5 6 1 38,8 8 8 1 35,6 7 25 0,000701 254692843 Dnahc10 dynein heavy chain 10, axonemal 1 2,7 11 15 0,9999 0,7 3 3 1 4,9 20 20 1 5,9 24 36 0,0000662 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, 254692859 NDUFA9 mitochondrial precursor 1 23,3 7 13 1 14,9 6 9 1 26 9 9 1 28,1 10 40 0,000851 254692892 Slc25a31 ADP/ATP translocase 4 1 43,4 15 74 1 34,1 10 55 1 43,1 14 14 1 43,4 15 211 0,0034718 254826790 slc25a10 mitochondrial dicarboxylate carrier 1 29,6 8 14 1 15,7 4 9 1 27,2 7 7 1 34,8 9 31 0,00086 254826802 4930511I11Rik armadillo repeat-containing protein 12 1 12,6 3 9 1 15,3 3 4 1 31,2 7 7 1 31,2 7 30 0,00106443 254910995 Naglu alpha-N-acetylglucosaminidase precursor 1 24,2 14 18 1 16,1 10 20 1 36 21 21 1 32,6 19 68 0,000987 254911018 ATP6V1E2 V-type proton ATPase subunit E 2 1 11,9 3 3 0,9999 12,4 3 3 1 18,1 4 4 1 25,7 6 10 0,000304 255069715 LAP3 cytosol aminopeptidase 1 36 14 22 1 22 9 20 1 43,7 18 18 1 42,4 17 100 0,00137688 255069795 vars valine--tRNA ligase 1 8,9 9 9 1 2,8 3 3 1 13,3 13 13 1 16,5 16 27 0,000188 255683347 CCDC151 coiled-coil domain-containing protein 151 isoform 2 1 23,1 11 13 1 8,4 4 8 1 17,9 9 9 1 24,8 12 38 0,00044 255683447 LOC100046727 IQ and ubiquitin-like domain-containing protein 1 6,9 4 5 - 0 0 0 1 8,5 6 6 1 11,9 8 14 0,000158 succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, 255958286 suclg1 mitochondrial precursor 1 32,7 9 43 1 27,5 6 17 1 38,2 13 13 1 35,5 12 157 0,0060514 256000803 1700006E09Rik uncharacterized protein C17orf105 homolog 1 23,2 3 6 1 29,3 4 12 1 29,3 4 4 1 29,3 4 27 0,00103471 256220343 Dhrs4 dehydrogenase/reductase SDR family member 4 isoform 1 1 31,2 6 10 1 30,8 6 13 1 43,7 10 10 1 44,1 11 47 0,00123623 256665241 zan zonadhesin precursor 1 6,3 25 50 1 4,3 18 35 1 17,3 68 68 1 16,9 65 200 0,000437 256773234 Dnahc1 dynein, axonemal, heavy chain 1 1 2,1 7 6 - 0,3 1 2 1 6,4 20 20 1 6,2 19 28 0,0000636 256818776 Wdr52 WD repeat-containing protein 52 1 5,8 8 13 1 4 6 9 1 12 17 17 1 11,5 17 48 0,000274 257467659 Dnahc6 axonemal dynein heavy chain 1 3,6 13 14 0,9991 0,5 2 2 1 7,5 26 26 1 7 24 45 0,0000939 257743039 LdhA L-lactate dehydrogenase A chain isoform 2 1 36 11 46 1 15,7 5 12 1 48,5 15 15 1 48,5 15 108 0,00183448 dihydrolipoyllysine-residue acetyltransferase component of pyruvate 257796245 dlaT dehydrogenase complex, mitochondrial 1 40,8 18 153 1 25,7 11 52 1 47 21 21 1 42,1 20 425 0,00580259

257900479 CATSPERB cation channel sperm-associated protein subunit beta precursor 1 14,4 13 23 1 3,9 4 6 1 11,8 12 12 1 16,3 15 44 0,000407 260064069 WDR65 WD repeat-containing protein 65 1 16,7 16 26 1 8,4 8 13 1 25,5 24 24 1 27,9 26 75 0,000481 260593706 Heatr7b2 maestro heat-like repeat-containing protein family member 2B 1 12 17 26 1 4,6 6 8 1 20,2 27 27 1 18,6 24 68 0,000391 261245029 CCDC146 coiled-coil domain containing 146 1 8 8 9 0,9987 2,1 2 2 - 1,1 1 1 1 8,2 8 12 0,000098

261245039 TMEM146 cation channel sperm-associated protein subunit delta precursor 1 9,9 5 6 - 1,6 1 1 1 14,2 8 8 1 11,2 6 14 0,000257 261824000 Psma3 proteasome subunit alpha type-3 1 25,1 5 10 1 21,2 4 8 1 23,9 6 6 1 28,6 6 26 0,000692 Réplica 1 Réplica 2 Réplica 3 Datos combinados

261862282 SLC2A3 solute carrier family 2, facilitated glucose transporter member 3 1 18,1 9 56 1 14,4 6 21 1 14 7 7 1 18,1 9 137 0,00542572 261862311 CCDC11 coiled-coil domain-containing protein 11 1 19,5 8 11 1 7,2 3 8 1 23,9 10 10 1 23,9 10 33 0,000435 262050574 EFHC2 EF-hand domain-containing family member C2 1 17,6 10 22 0,9994 3,2 2 2 1 41,9 24 24 1 38,7 22 70 0,000824 262072996 ZPBP2 zona pellucida-binding protein 2 isoform 1 precursor 1 23,5 5 8 0,9999 14,3 3 4 1 27 8 8 1 35,3 10 37 0,00108516 262231811 Dynlt1b dynein light chain Tctex-type 1F isoform 1 1 24,5 2 3 - 15,1 1 1 0,9995 24,5 2 2 1 24,5 2 6 0,000568 262359961 1700101E01Rik uncharacterized protein LOC329375 isoform 1 1 19,6 4 6 0,9979 7,7 2 2 1 18,1 4 4 1 23,2 5 20 0,000552 coiled-coil-helix-coiled-coil-helix domain-containing protein 6, 267844804 CHCHD6 mitochondrial isoform 1 1 36,7 8 17 1 27,3 6 12 1 45,7 11 11 1 45,7 11 46 0,001404 268370090 WDR64 WD repeat-containing protein 64 1 3 3 3 0,9983 2,4 2 2 1 9,5 9 9 1 11,7 11 18 0,000146 268370161 1700026D08Rik uncharacterized protein C15orf26 homolog 1 29,4 7 9 1 15,2 4 10 1 40,3 9 9 1 40,3 9 38 0,00121906 268370191 NT5C1B cytosolic 5'-nucleotidase 1B 1 43,8 19 76 1 25 12 56 1 53,2 24 24 1 48,5 22 292 0,00463314 268370211 Rnls renalase isoform 1 1 35 7 12 1 21,7 5 13 1 60,7 12 12 1 60,7 12 94 0,00424702 268370275 Gm884 uncharacterized protein LOC380730 1 8,9 13 27 1 6,3 9 14 1 13,6 19 19 1 13,1 18 104 0,000807 268839833 TTC29 tetratricopeptide repeat protein 29 1 7,6 3 5 1 6,2 2 5 0,9989 5,1 2 2 1 7,6 3 11 0,000186 269784615 atp2b4 plasma membrane calcium ATPase 4 isoform a 1 29,8 28 59 1 18,4 16 29 1 29,8 29 29 1 29,2 27 179 0,00160778 269784687 Slc25a21 mitochondrial 2-oxodicarboxylate carrier isoform 1 1 27,2 8 12 1 13,8 4 8 1 24,5 7 7 1 27,2 8 29 0,000681 mycophenolic acid acyl-glucuronide esterase, mitochondrial 269784760 ABHD10 isoform 1 precursor 1 24,7 8 8 1 16,8 5 7 1 38 10 10 1 40,1 11 30 0,000712 269784762 cbr4 carbonyl reductase family member 4 1 32,6 6 14 0,9999 15,3 3 4 1 43,2 8 8 1 43,2 8 38 0,00120132 269973935 Csl citrate synthase-like protein 1 44,8 15 81 1 32,8 11 28 1 50,2 18 18 1 50,2 18 244 0,00437562 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 27228985 NDUFA12 12 1 32,9 4 10 1 56,4 6 14 1 70,5 9 9 1 70,5 9 45 0,00219103 27229101 5730469M10Rik redox-regulatory protein FAM213A 1 26,1 7 9 1 13,8 3 3 1 21,6 5 5 1 26,1 7 20 0,000641 27369533 LOC100039128 nuclear pore complex protein Nup93 1 5,5 4 4 1 4 3 4 1 5,5 5 5 1 8,7 7 13 0,000119

27370380 - probable isocitrate dehydrogenase [NAD] gamma 2, mitochondrial 1 17,4 6 7 1 9,6 3 5 1 28,8 8 8 1 28,8 8 34 0,000868 27502349 PMPCA mitochondrial-processing peptidase subunit alpha precursor 1 11,6 6 7 1 7,4 4 6 1 5,2 3 3 1 14,1 8 16 0,000279 ATP-binding cassette sub-family B member 8, mitochondrial 27753995 abcb8 precursor 1 11,2 7 7 0,9985 4,3 2 2 1 14,1 8 8 1 17,7 10 19 0,000248 27754103 Psmc6 26S protease regulatory subunit 10B 1 15,2 3 3 0,9992 3,1 1 2 1 20,8 7 7 1 28,5 8 12 0,000214 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, 27754144 Ndufb5 mitochondrial precursor 1 23,8 4 11 1 19,6 3 4 1 28 5 5 1 28 5 26 0,00109781 27923590 Adam4 a disintegrin and metallopeptidase domain 4 precursor 1 6,3 4 5 - 3,5 2 2 1 11,9 8 8 1 13,4 8 19 0,000234 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, 281485615 NDUFS4 mitochondrial 1 37,1 6 8 - 0 0 0 1 37,1 6 6 1 37,1 6 20 0,000677 28173550 sept7 septin-7 isoform 1 1 23,6 9 9 1 11,5 3 3 1 28,2 9 9 1 28,4 10 25 0,000409 28201978 LOC100044117 pyruvate dehydrogenase protein X component, mitochondrial 1 29,9 11 36 1 23,6 7 20 1 35,5 12 12 1 35,5 12 139 0,00248087 283046757 HEATR7A HEAT repeat containing 7A isoform 1 1 9,5 14 16 1 3,9 5 8 1 6,3 9 9 1 10,9 16 34 0,000208 28316764 ANKRD5 ankyrin repeat and EF-hand domain-containing protein 1 1 11,1 7 9 1 7,5 5 8 1 6,5 4 4 1 13,8 9 27 0,00026 283837762 Dnahc17 dynein heavy chain 17, axonemal 1 13,2 47 82 1 2,4 10 14 1 17,8 64 64 1 19,5 70 185 0,000403 285401645 1700001C19Rik uncharacterized protein LOC75462 isoform 2 1 40 4 4 - 0 0 0 1 40 4 4 1 40 4 8 0,000469 29243946 Thnsl1 threonine synthase-like 1 isoform a 1 28,8 16 19 1 11,2 6 9 1 30,8 17 17 1 40,4 22 47 0,000547 29244338 4933400C05Rik spermatogenesis-associated protein 32 1 15 3 3 - 0 0 0 1 15,9 4 4 1 26,3 6 8 0,000259 293597567 Aldoa fructose-bisphosphate aldolase A isoform 1 precursor 1 63,4 19 39 1 60,5 18 31 1 76,8 24 24 1 81,8 25 110 0,00138208 29366814 Fahd2a fumarylacetoacetate hydrolase domain-containing protein 2A 1 59,7 12 20 1 29,1 7 9 1 64,9 14 14 1 64,9 14 61 0,00193565 294460010 LOC69439 uncharacterized protein LOC69439 1 3,6 3 3 - 0 0 0 0,9989 2,6 2 2 1 3,6 3 4 0,0000453 295317325 snap23 synaptosomal-associated protein 23 isoform a 1 18,6 3 3 0,999 11,9 2 3 - 6,7 1 1 1 18,6 3 6 0,000181 295444814 Prss46 serine protease 46 1 20,4 6 18 1 26,4 7 22 1 20,4 6 6 1 26,4 7 70 0,00295875 295789144 - UPF0704 protein C6orf165 homolog 1 10,3 5 5 - 0 0 0 1 5,2 3 3 1 9,7 5 6 0,000108 29648322 DNAJB13 dnaJ homolog subfamily B member 13 1 18,4 4 5 - 3,2 1 1 1 35,4 9 9 1 33,2 8 30 0,000697 297747362 zpbp1 zona pellucida-binding protein 1 isoform 2 1 35,9 11 34 1 34,5 10 30 1 53,8 15 15 1 55,8 16 151 0,00444936

29789148 NDUFB9 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 1 51,4 6 9 1 51,4 6 8 1 56,4 7 7 1 65,4 8 34 0,0017087 29789217 TSNAXIP1 translin-associated factor X-interacting protein 1 1 4,7 3 4 1 5,4 3 6 1 7,7 5 5 1 7,7 5 15 0,000166 29789289 Echs1 enoyl-CoA hydratase, mitochondrial precursor 1 22,1 5 6 1 22,1 5 5 1 29 7 7 1 33,1 9 23 0,000649 298231198 Nup53 nucleoporin NUP53 isoform 2 1 15,3 3 3 1 15,3 3 5 1 13 3 3 1 19,2 4 11 0,000413

298676450 Carkd ATP-dependent (S)-NAD(P)H-hydrate dehydratase isoform 1 1 30 6 6 0,9978 3,7 1 2 1 29,1 6 6 1 33,6 7 15 0,000349 30172570 DNALI1 axonemal dynein light intermediate polypeptide 1 1 40,3 9 24 1 18,6 5 11 1 55,4 14 14 1 55,4 14 81 0,00210678 30410018 Iqcg IQ domain-containing protein G 1 7,6 3 6 0,9991 5 2 5 1 7,6 3 3 1 7,6 3 20 0,000444 30424705 Tmem177 transmembrane protein 177 1 8,4 3 4 - 0 0 0 1 8,4 3 3 1 14,5 5 8 0,000244 30425048 LDHAL6B L-lactate dehydrogenase A-like 6B 1 46,3 13 126 1 47,6 12 92 1 61 15 15 1 65,2 17 391 0,00619643 30425148 CCDC40 coiled-coil domain-containing protein 40 1 12,2 11 19 1 6,2 6 11 1 16 16 16 1 18,9 18 61 0,000505 30425270 FAM78A protein FAM78A 1 14,1 3 4 - 6,7 1 1 0,9997 9,9 2 2 1 14,1 3 9 0,000388 30519943 Samm50 sorting and assembly machinery component 50 homolog 1 46,7 15 17 1 16,8 6 7 1 34,8 12 12 1 46,7 15 42 0,000755 30520019 sccpdh saccharopine dehydrogenase-like oxidoreductase 1 49,7 13 54 1 17,9 6 15 1 46,9 14 14 1 51,3 14 153 0,00338101 307344688 Wdr96 WD repeat domain 96 1 2,1 3 5 0,9988 1,1 2 3 1 6,4 8 8 1 7,4 10 17 0,0000996 30794150 man2c1 alpha-mannosidase 2C1 1 6,6 5 5 0,9992 2,4 2 2 1 14,1 12 12 1 16,7 13 19 0,000211

30794476 PRKAR1A cAMP-dependent protein kinase type I-alpha regulatory subunit 1 24,1 9 13 1 16,8 6 9 1 24,7 8 8 1 29,4 11 40 0,000914 309262133 Akd1 PREDICTED: adenylate kinase domain-containing protein 1 1 4,7 8 9 1 2,3 4 4 1 4 7 7 1 6,5 11 26 0,000127 Réplica 1 Réplica 2 Réplica 3 Datos combinados

31543728 SLC2A5 solute carrier family 2, facilitated glucose transporter member 5 1 10,6 6 18 1 7,4 3 5 1 10,8 6 6 1 10,8 7 51 0,00215798 31560239 ASRGL1 isoaspartyl peptidase/L-asparaginase 1 43,6 11 33 1 31,9 9 15 1 47,5 12 12 1 47,5 12 81 0,00163761 31560590 Fhl4 four and a half LIM domains 4 1 15,8 4 5 0,9991 7,5 2 2 1 50,5 11 11 1 46,6 10 23 0,000669 31560686 Hspa2 heat shock-related 70 kDa protein 2 1 35,7 17 35 1 28,3 14 15 1 42,5 21 21 1 42,5 21 127 0,00150317 31560705 ACSL1 long-chain-fatty-acid--CoA ligase 1 1 32,6 21 60 1 18,5 13 25 1 43,9 25 25 1 43,3 25 175 0,00215752 31560731 Atp6v1a V-type proton ATPase catalytic subunit A 1 8,4 4 4 1 8,6 4 4 1 8,4 4 4 1 11,8 6 14 0,000194 31980648 ATP5B ATP synthase subunit beta, mitochondrial precursor 1 66,2 22 198 1 58,4 19 185 1 71,6 23 23 1 71,6 23 668 0,00822562 31980744 Gm4045 ATP synthase subunit g, mitochondrial 1 40,8 3 7 1 33 2 3 1 49,5 4 4 1 40,8 3 15 0,00132187 31980762 Sod2 superoxide dismutase [Mn], mitochondrial precursor 1 25,7 5 10 0,9991 9,5 2 4 1 25,7 5 5 1 25,7 5 29 0,00132393 31980939 1700020C11Rik mitochondrial fission process protein 1 1 21,1 3 8 0,9995 14,5 2 4 1 21,7 4 4 1 21,7 4 16 0,000852 31980942 IMPA1 inositol monophosphatase 1 1 37,2 9 11 1 12,6 3 6 1 44 11 11 1 48,7 12 31 0,000896 31980998 acot9 acyl-coenzyme A thioesterase 9, mitochondrial 1 43,5 18 48 1 19,1 8 18 1 43,7 17 17 1 50,8 21 149 0,00267039

31981600 Ndufa2 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 1 41,4 4 4 1 33,3 3 3 1 33,3 3 3 1 41,4 4 11 0,00066 31981810 dci enoyl-CoA delta isomerase 1, mitochondrial precursor 1 21,5 5 7 1 11,8 3 5 1 24,9 6 6 1 24,9 6 21 0,000628 31982091 Gm12906 mitochondrial import receptor subunit TOM22 homolog 1 22,5 2 3 1 33,1 3 6 1 31 3 3 1 47,9 4 14 0,00108981 31982186 mdh2 malate dehydrogenase, mitochondrial precursor 1 58,3 15 38 1 47,6 12 35 1 58,3 15 15 1 58,3 15 189 0,00401475 31982332 GLUL glutamine synthetase 1 14,7 6 7 0,9999 8,3 3 4 1 32,4 11 11 1 32,4 11 43 0,00111421 31982710 FSCN3 fascin-3 1 13,5 5 7 1 13,7 5 7 1 7 3 3 1 13,7 5 18 0,000413 31982856 dld dihydrolipoyl dehydrogenase, mitochondrial precursor 1 40,7 13 72 1 25 8 33 1 47 16 16 1 47 16 219 0,00411416 33147082 bat3 large proline-rich protein BAG6 isoform 1 1 15,5 12 15 1 9,8 7 12 1 7,8 5 5 1 18,2 14 37 0,000606 33354251 Lypd4 ly6/PLAUR domain-containing protein 4 precursor 1 39 7 16 1 26 4 10 1 37,8 7 7 1 43,9 8 52 0,00232552 33468873 ACE angiotensin-converting enzyme isoform 2 precursor 1 29,8 19 47 1 18,3 11 32 1 35,4 22 22 1 35,8 22 150 0,0022125

33468937 TIMM17B mitochondrial import inner membrane translocase subunit Tim17-B 1 24,4 3 4 1 21,5 3 4 0,9995 12,8 2 2 1 33,1 4 10 0,000532 33469015 prcp lysosomal Pro-X carboxypeptidase precursor 1 6,3 3 3 1 4,9 2 6 1 17,5 7 7 1 16,1 6 18 0,000481 33563282 PSMA1 proteasome subunit alpha type-1 1 25,1 6 6 1 23,6 5 6 1 44,5 11 11 1 56,7 14 26 0,000915 336088630 Nit1 nitrilase homolog 1 isoform 2 1 25,9 6 9 1 21 5 11 1 34,5 8 8 1 42,4 10 27 0,000801 33859482 Gm13050 elongation factor 2 1 5,2 4 4 - 1,2 1 1 1 9,1 7 7 1 10,3 8 12 0,000107 33859604 PSMC2 26S protease regulatory subunit 7 1 15,4 6 6 1 12,2 5 11 1 9,9 4 4 1 24 10 25 0,000319 33859638 THEG testicular haploid expressed gene protein isoform 1 1 32,5 11 30 1 13,4 5 25 1 40,2 13 13 1 38,2 13 92 0,0020675 33859811 HADHA trifunctional enzyme subunit alpha, mitochondrial precursor 1 37,9 22 52 1 17,6 12 23 1 46 28 28 1 43,5 26 180 0,0018836 33942057 SPINT4 kunitz-type protease inhibitor 4 precursor 1 25,2 3 5 - 0 0 0 0,9995 17,6 2 2 1 25,2 3 8 0,000402 34303985 CCDC63 coiled-coil domain-containing protein 63 1 26,1 13 28 0,9999 4,6 3 3 1 23,9 13 13 1 27,7 16 76 0,000973 34328230 Ak2 adenylate kinase 2, mitochondrial isoform b 1 37,5 7 20 1 36,2 7 17 1 38,8 8 8 1 42,2 9 74 0,00192146 succinate dehydrogenase [ubiquinone] iron-sulfur subunit, 34328286 LOC638710 mitochondrial precursor 1 49,3 12 26 1 19,1 5 12 1 46,8 11 11 1 49,3 12 69 0,0014289 34536823 Cox6b2 cytochrome c oxidase subunit 6B2 isoform 2 1 47,7 4 5 1 38,6 3 4 1 47,7 4 4 1 47,7 4 18 0,00166168 359339012 Smrp1 spermatid-specific manchette-related protein 1 isoform 3 1 47,3 13 31 0,9992 28,7 7 11 1 67,6 17 17 1 67,6 17 6 0,000121 359385696 Ywhaz 14-3-3 protein zeta/delta isoform 1 1 43,7 9 18 1 35,3 5 11 1 49 12 12 1 48,6 11 58 0,00170883 359807367 Pkm pyruvate kinase, muscle isoform M1 1 69,1 30 198 1 43,7 19 97 1 69,1 29 29 1 69,1 30 627 0,00639532 37574048 Gm6293 cytochrome b-c1 complex subunit 9 1 37,5 2 4 0,999 37,5 2 2 1 37,5 2 2 1 37,5 2 11 0,0014838 377833423 C8orfk29 PREDICTED: putative transmembrane protein C8orfK29 1 15,9 3 4 1 14,3 3 3 - 6,1 1 1 1 20,4 4 8 0,000242 38142460 LOC100045699 electron transfer flavoprotein subunit beta 1 15,7 3 3 0,9991 11,4 2 2 1 52,2 11 11 1 48,6 10 18 0,000426 pyruvate dehydrogenase phosphatase regulatory subunit, 38142488 4930402E16Rik mitochondrial precursor 1 11,4 9 9 1 4 4 4 1 5 4 4 1 13,4 11 19 0,000216 38348482 CCT8L1 chaperonin containing TCP1, subunit 8 (theta)-like 1 1 14,9 7 8 1 5,3 3 3 1 13,3 7 7 1 21,5 11 22 0,000395 384871588 Ace3 angiotensin I converting enzyme 3 precursor 1 16,3 11 22 1 9 6 16 1 30 18 18 1 24,8 15 66 0,000922 38490690 iars2 isoleucine--tRNA ligase, mitochondrial precursor 1 18,8 16 32 1 9 7 12 1 29 24 24 1 27,2 23 84 0,000797 40254361 Hspa4l heat shock 70 kDa protein 4L 1 13,8 9 10 0,9987 3,3 2 2 1 14 9 9 1 18,7 12 23 0,000211 40254507 PGP phosphoglycolate phosphatase 1 41,1 11 12 1 26,5 7 9 1 40,2 11 11 1 44,2 12 35 0,000818 40254574 FABP9 fatty acid-binding protein 9 1 77,3 10 57 1 56,1 7 39 1 77,3 10 10 1 77,3 10 145 0,00445014 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, 406855423 Ndufs8 mitochondrial 1 26,4 5 11 1 13,7 2 5 1 29,7 6 6 1 29,7 6 29 0,00107062 407027856 Capzb F-actin-capping protein subunit beta isoform c 1 47,2 11 22 1 29,2 7 14 1 43,9 11 11 1 47,2 12 62 0,00140794 407260963 C1orf101 PREDICTED: uncharacterized protein C1orf101-like 1 6,7 4 4 - 3,7 2 3 1 7,5 5 5 1 6,7 4 10 0,000129 407261206 Nsun4 PREDICTED: sperm head and tail associated protein-like 1 12,7 7 7 0,9993 3,1 2 2 1 13,9 8 8 1 13,9 8 21 0,000396 407261678 LOC77908 PREDICTED: uncharacterized protein LOC77908 1 54,8 4 9 1 47,3 4 7 1 54,8 4 4 1 55,9 5 34 0,00324911 407261996 Serpina1 PREDICTED: alpha-1-antiproteinase-like 1 16 5 12 1 6,5 2 14 1 26,6 10 10 1 24,6 9 57 0,00146545 Réplica 1 Réplica 2 Réplica 3 Datos combinados

483968030 Prkaca cAMP-dependent protein kinase catalytic subunit alpha isoform 2 1 29,4 9 13 1 17,2 6 9 1 39,1 12 12 1 44 15 46 0,00111135 49355801 Gykl1 glycerol kinase-like 1 1 48,3 18 48 1 16,8 6 12 1 48,1 18 18 1 51,9 20 115 0,00210232 50582595 CCIN calicin 1 32,5 16 36 1 12,9 7 16 1 42,7 21 21 1 42,3 21 104 0,00150565 50582603 Defb22 beta-defensin 22 precursor 1 9,5 2 10 1 5,6 2 10 1 10,1 3 3 1 10,1 3 38 0,00193442 51491845 CLTC clathrin heavy chain 1 1 5,4 6 6 1 3,4 4 4 1 5,1 5 5 1 11,3 13 15 0,0000858 51556221 Ces7 carboxylesterase 5A precursor 1 8,3 3 4 - 0 0 0 1 15,7 7 7 1 15,7 7 18 0,000522 51591899 PPP3R2 calcineurin subunit B type 2 1 19 3 4 0,999 12,8 2 4 0,9999 12,8 2 2 1 19 3 12 0,000481 51921341 AA467197 normal mucosa of esophagus-specific gene 1 protein 1 48,2 5 12 1 48,2 4 7 1 49,4 6 6 1 49,4 7 36 0,00432518 52138534 BC048562 uncharacterized protein LOC434439 1 50 5 6 - 0 0 0 1 44,6 5 5 1 50 5 16 0,00108912 succinate dehydrogenase [ubiquinone] flavoprotein subunit, 54607098 SDHA mitochondrial precursor 1 52,9 23 48 1 45,9 19 48 1 58,7 25 25 1 62,2 27 213 0,00281903 55741460 PARK7 protein DJ-1 1 29,6 3 4 1 19,6 3 4 1 53,4 8 8 1 53,4 8 17 0,000628 57222276 Adam6b a disintegrin and metalloproteinase domain 6-like 1 8,2 5 12 1 7,3 4 6 1 9,5 6 6 1 14,4 9 28 0,000367

58037109 NDUFB10 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 1 51,7 8 11 1 28,4 4 6 1 51,7 8 8 1 51,7 8 29 0,00100555 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, 58037117 Gm12251 mitochondrial precursor 1 40,7 12 29 1 28,1 7 18 1 46 13 13 1 46 14 79 0,00223837 58037189 1700026L06Rik uncharacterized protein C9orf9 homolog 1 16,1 2 9 1 30,4 3 7 1 52,4 6 6 1 38,7 4 30 0,00169254 58037223 Lpcat2b lysophosphatidylcholine acyltransferase 2B 1 7,2 3 3 - 1,7 1 1 0,9998 5,6 2 2 1 8,9 4 7 0,000114

58037235 OXSM 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial precursor 1 35,7 9 11 1 20,3 5 8 1 37,3 10 10 1 43,4 11 37 0,00079 58037359 1700011F03Rik testis-expressed sequence 37 protein 1 34,4 5 10 0,9994 6,1 1 4 1 34,4 5 5 1 34,4 5 22 0,00121422 58037363 SPATC1 speriolin 1 14,2 5 19 1 13,8 4 17 1 18,1 7 7 1 20,2 7 71 0,0019912 58037395 SCAMP1 secretory carrier-associated membrane protein 1 1 13 3 8 0,9989 10,7 2 2 1 10,7 2 2 1 16 4 22 0,000961 58037409 Phpt1 14 kDa phosphohistidine phosphatase 1 19,4 2 4 - 9,7 1 1 0,9998 9,7 1 1 1 19,4 2 8 0,000414 58037413 morn5 MORN repeat-containing protein 5 1 27,6 3 4 1 35,9 4 6 1 55,9 6 6 1 66,5 7 17 0,000741 58037457 Arpm1 actin-related protein T3 1 10,8 3 3 1 13,8 4 6 1 33,1 7 7 1 36,3 8 18 0,000461 58037499 LCN12 epididymal-specific lipocalin-12 precursor 1 10,4 2 5 0,9991 5,7 1 4 0,9998 5,7 1 1 1 10,4 2 14 0,00112252 59709445 4930579C15Rik equatorin precursor 1 19,3 5 20 1 16,6 4 17 1 27,6 6 6 1 27,6 6 68 0,00243414 61316404 Odf3b outer dense fiber protein 3B 1 27,3 5 7 1 19,7 4 12 1 33,2 6 6 1 31,1 6 29 0,000923 61888838 HSD17B10 3-hydroxyacyl-CoA dehydrogenase type-2 1 29,5 4 6 1 29,5 4 7 1 54,4 10 10 1 45,2 8 28 0,0007 62000656 Fam71b protein FAM71B 1 23,3 14 38 1 18 9 29 1 27,7 16 16 1 27,5 15 143 0,00259935 62078759 tekt3 tektin-3 1 46,7 20 45 1 28,6 12 27 1 50,6 23 23 1 50,6 23 162 0,00241248 65301151 Izumo1 izumo sperm-egg fusion protein 1 precursor 1 37 12 45 1 14,9 5 27 1 44,1 16 16 1 38,8 13 140 0,00343718 66392180 Pacrg parkin coregulated gene protein homolog 1 37,8 8 24 1 39,4 7 19 1 46,5 9 9 1 48,1 9 74 0,00246645 6671509 ACTB actin, cytoplasmic 1 1 41,3 11 27 1 36,5 9 37 1 69,1 20 20 1 69,1 20 80 0,00125081 6671549 Prdx6 peroxiredoxin-6 1 39,3 6 4 - 0 0 0 1 50 8 8 1 50 8 12 0,000324 6671569 Gm9093 60S acidic ribosomal protein P0 1 14,5 4 4 - 3,5 1 1 1 34,4 7 7 1 37,9 8 14 0,000392 6671678 Car4 carbonic anhydrase 4 precursor 1 39,3 9 23 1 31,1 6 16 1 34,1 7 7 1 36,7 8 58 0,00191375 6671702 cct5 T-complex protein 1 subunit epsilon 1 9,6 5 7 - 0 0 0 1 17,9 8 8 1 19,8 9 20 0,000302 6678293 Prss39 inactive serine protease 39 precursor 1 17,2 6 16 1 10,6 3 9 1 32,2 9 9 1 32,2 9 40 0,00131269 6678419 tpp2 tripeptidyl-peptidase 2 1 25,3 24 26 1 7,1 8 14 1 30,1 29 29 1 34,2 31 74 0,000527 6678465 Tuba3a tubulin alpha-3 chain 1 52,4 19 42 1 50 15 49 1 66,4 23 23 1 66,4 23 136 0,00187254 6678553 vamp3 vesicle-associated membrane protein 3 1 55,3 5 9 1 47,6 3 11 1 54,4 4 4 1 54,4 4 32 0,0030369 6678908 MNS1 meiosis-specific nuclear structural protein 1 1 12,2 7 10 1 11,6 6 10 1 17,3 9 9 1 22,4 12 34 0,000443 high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic 6679249 PDE8A phosphodiesterase 8A 1 9 4 4 - 0 0 0 - 0 0 0 1 6,7 3 3 0,0000351 pyruvate dehydrogenase E1 component subunit alpha, somatic 6679261 Pdha1 form, mitochondrial precursor 1 6,9 3 5 1 10,5 4 13 1 15,4 6 6 1 19,7 8 33 0,000585 pyruvate dehydrogenase E1 component subunit alpha, testis- 6679263 pdhA2 specific form, mitochondrial precursor 1 63,2 21 103 1 40,7 13 95 1 70,3 21 21 1 63,2 21 377 0,00758522 Réplica 1 Réplica 2 Réplica 3 Datos combinados

6753498 COX4I1 cytochrome c oxidase subunit 4 isoform 1, mitochondrial precursor 1 43,8 7 66 1 39,1 6 78 1 43,8 8 8 1 43,8 8 215 0,00842119 6753540 csnk2a2 casein kinase II subunit alpha' 1 52 14 21 1 33,1 9 11 1 56,6 14 14 1 59,7 16 68 0,00181251 6753560 Cyct cytochrome c, testis-specific 1 41,9 5 5 0,9987 14,3 2 2 1 41,9 5 5 1 41,9 5 14 0,00082 6753618 ddt D-dopachrome decarboxylase 1 60,2 5 6 0,9995 22,9 2 2 1 76,3 7 7 1 66,9 6 17 0,000946 6754000 Gk2 glycerol kinase 2 1 36,6 15 24 0,9992 4,2 2 6 1 38,1 16 16 1 38,1 16 82 0,00131217 6754036 Got2 aspartate aminotransferase, mitochondrial 1 17,9 7 8 1 12,3 5 20 1 36 12 12 1 38,1 13 50 0,000996 6754084 Gm5562 glutathione S-transferase Mu 1 1 22 4 4 1 10,6 2 4 1 34,9 7 7 1 36,2 7 11 0,000309 6754086 GSTM5 glutathione S-transferase Mu 5 1 56,7 11 67 1 39,3 8 46 1 62,9 15 15 1 66,1 15 205 0,00397402 6754092 Gstz1 maleylacetoacetate isomerase isoform 1 1 22,2 4 4 - 0 0 0 1 35,2 6 6 1 45,8 8 12 0,000453 6754254 HSP90AA1 heat shock protein HSP 90-alpha 1 13,2 10 10 1 9,1 7 7 1 22,6 17 17 1 22,9 16 40 0,000467 6754696 Gm16379 macrophage migration inhibitory factor 1 23,5 3 3 - 7,8 1 1 0,9992 15,7 2 2 0,9996 17,4 2 4 0,000457 6755000 pdcd6 programmed cell death protein 6 1 17,8 3 3 - 5,8 1 1 0,9994 12,6 2 2 1 17,8 3 4 0,000193 6755114 Prdx5 peroxiredoxin-5, mitochondrial precursor 1 45,2 8 14 1 25,2 4 7 1 32,4 8 8 1 45,7 10 42 0,00117855 6755120 38231 septin-4 1 15,7 6 11 - 1,7 1 1 1 22,8 9 9 1 22,8 9 38 0,000643 6755196 Psma4 proteasome subunit alpha type-4 1 13,8 4 6 0,9999 15,7 3 3 1 29,1 6 6 1 29,1 6 15 0,000475 6755198 Psma6 proteasome subunit alpha type-6 1 24 5 8 1 19,1 4 7 1 43,5 9 9 1 43,5 9 27 0,000784 6755202 PSMB3 proteasome subunit beta type-3 1 32,2 4 7 1 22 3 4 1 34,1 5 5 1 42 6 16 0,000787 6755204 PSMB5 proteasome subunit beta type-5 1 43,6 8 16 1 43,2 9 21 1 37,9 8 8 1 52,3 10 53 0,00155853 6755368 LOC100047329 40S ribosomal protein S18 1 19,1 3 5 0,9993 13,2 2 4 0,9995 11,2 2 2 1 24,3 4 11 0,000423 6755372 rps3 40S ribosomal protein S3 1 23 5 5 1 18,1 4 5 1 31,7 7 7 1 28,4 6 17 0,000371 6755536 slc16a7 monocarboxylate transporter 2 1 3,9 2 5 0,9988 3,9 2 2 - 1,9 1 1 0,9995 3,9 2 8 0,000495 6755560 SLC4A1 band 3 anion transport protein 1 9,9 7 8 1 11,1 8 10 1 16,5 12 12 1 16,5 13 51 0,0010201 6755614 Spa17 sperm surface protein Sp17 1 34,2 4 15 1 24,8 3 9 1 34,2 4 4 1 34,2 4 36 0,00161908 6755863 Hsp90b1 endoplasmin precursor 1 8,4 6 6 1 8,7 6 6 1 15,5 11 11 1 23,2 15 31 0,00031 6755963 VDAC1 voltage-dependent anion-selective channel protein 1 1 12,4 3 4 - 3,9 1 1 1 41,3 8 8 1 45,2 9 17 0,000391 6755965 vdac2 voltage-dependent anion-selective channel protein 2 1 49,5 10 30 1 28,8 6 8 1 59,7 13 13 1 56,9 12 108 0,00250379 6755967 Gm7591 voltage-dependent anion-selective channel protein 3 isoform 2 1 45,9 8 18 - 0 0 0 1 50,2 10 10 1 50,2 10 62 0,0015403 67625729 Gm128 protein MENT precursor 1 45,1 10 59 1 39,7 9 76 1 45,1 11 11 1 45,1 11 220 0,00812237 67846103 SHMT1 serine hydroxymethyltransferase, cytosolic 1 9,6 4 6 - 1,9 1 1 1 18,2 8 8 1 18,8 8 13 0,000227 70778976 Pgk1-rs7 phosphoglycerate kinase 1 1 15,1 5 5 1 11 3 9 1 21,1 7 7 1 21,1 7 25 0,000396 7106277 CSNK2B casein kinase II subunit beta 1 41,4 6 10 1 16,3 3 5 1 34,9 6 6 1 45,6 7 24 0,00130153 7106335 KRT17 keratin, type I cytoskeletal 17 1 17,3 8 8 - 0 0 0 - 0 0 0 1 17,3 8 8 0,000176 7106387 psma5 proteasome subunit alpha type-5 1 22,4 4 6 0,9989 10,4 2 2 1 25,7 5 5 1 22,4 4 15 0,000614 7110703 PSMC5 26S protease regulatory subunit 8 1 12,1 4 5 0,999 6,2 2 10 1 8,4 3 3 1 14,3 5 19 0,000306 7242197 psmb1 proteasome subunit beta type-1 precursor 1 31,7 6 7 1 27,5 5 7 1 46,7 8 8 1 46,7 8 23 0,000658 7305229 Ldhc L-lactate dehydrogenase C chain 1 71,4 19 242 1 63,9 16 109 1 75 21 21 1 75 21 716 0,01037002 74315965 Hyal5 hyaluronidase PH-20 1 11,2 6 7 1 4,9 2 3 1 19,2 9 9 1 23,4 11 22 0,000488 74315975 PSMD1 26S proteasome non-ATPase regulatory subunit 1 1 9,4 7 7 0,999 2,8 2 2 1 16,2 13 13 1 16,2 13 26 0,000261 75992911 Acsl6 long-chain-fatty-acid--CoA ligase 6 isoform 1 1 5,6 3 3 - 1,3 1 1 - 2,6 1 1 1 5,6 3 5 0,0000588 mitochondrial translocator assembly and maintenance protein 41 76559944 1500001M20Rik homolog precursor 1 13,6 3 4 - 3,9 1 1 1 12,5 4 4 1 18,4 5 9 0,000294 7656912 ATP8A2 probable phospholipid-transporting ATPase IB 1 3,3 3 3 0,9991 1,8 2 2 - 1 1 1 1 4 4 6 0,0000522 7657607 SPAG6 sperm-associated antigen 6 1 23,3 9 21 1 14,4 6 12 1 22,7 10 10 1 22,7 10 64 0,00124562 76781476 BC089491 selenoprotein V 1 14 3 3 1 14 3 3 1 14 3 3 1 14 3 9 0,000226 7710014 cul3 cullin-3 1 21,4 13 13 1 15,4 9 12 1 31,2 20 20 1 32,4 22 47 0,000399 7710018 stom erythrocyte band 7 integral membrane protein 1 35,2 8 23 1 20,1 4 15 1 32 7 7 1 35,2 8 57 0,00161192 78214312 Gm12231 ATP synthase subunit b, mitochondrial precursor 1 31,6 10 27 1 11,7 3 19 1 32,8 9 9 1 33,2 10 62 0,00168058 82904142 Gm1096 PREDICTED: CD177 antigen isoform 2 1 14,4 3 8 1 17,2 4 9 1 11,2 2 2 1 17,2 4 29 0,00115369 83627689 SLC22A14 solute carrier family 22 member 14 1 5,1 3 3 - 0 0 0 1 6,8 4 4 1 6,8 4 9 0,000218 83715998 Gm2972 ATP synthase subunit e, mitochondrial 1 60,6 4 6 0,9993 26,8 2 4 1 60,6 4 4 1 60,6 4 16 0,00129122 83816907 Gm15785 dnaJ homolog subfamily B member 6 isoform a 1 25,2 9 15 1 9,6 4 9 1 30,7 10 10 1 30,4 10 49 0,00123399 Réplica 1 Réplica 2 Réplica 3 Datos combinados

Probabilidad Números de Probabilidad Porcentaje Números de Probabilidad Números de Probabilidad Números de de Porcentaje de péptidos Número de de de péptidos Número de de Porcentaje de péptidos Número de de Porcentaje de péptidos Número de Valor Referencia GI Proteína Nombre de la proteína coincidencia cobertura únicos espectros coincidencia cobertura únicos espectros coincidencia cobertura únicos espectros coincidencia cobertura únicos espectros APEX putative ATP-dependent Clp protease proteolytic subunit, 8393156 clpP mitochondrial precursor 1 18 4 8 1 24,3 5 5 1 28,7 6 6 1 28,7 6 23 0,000739 8393988 PMM2 phosphomannomutase 2 1 12,8 3 3 - 5,4 1 1 1 18,2 4 4 1 21,1 5 8 0,000198 serine/threonine-protein phosphatase 2A catalytic subunit beta 8394024 PPP2CB isoform 1 12,6 3 3 - 4,5 1 1 1 30,7 7 7 1 30,7 7 12 0,00033 84794552 PEBP1 phosphatidylethanolamine-binding protein 1 1 70,6 8 25 1 34,2 4 11 1 82,9 10 10 1 88,8 11 81 0,00457182 84993736 acsbg2 long-chain-fatty-acid--CoA ligase ACSBG2 1 16,8 8 10 1 10 5 9 1 22,2 10 10 1 22,2 10 37 0,000614 85662408 Crat carnitine O-acetyltransferase 1 24,6 13 72 1 25,7 14 48 1 38,5 20 20 1 36,3 18 258 0,00377826 85701754 Ccdc114 coiled-coil domain-containing protein 114 1 11,7 6 8 1 12 6 12 1 5,6 3 3 1 18,2 9 37 0,0005 85701762 LOC100046792 uncharacterized protein C6orf163 homolog 1 15,2 4 4 0,999 7,9 2 3 1 33,8 9 9 1 30,2 8 21 0,000413 85701852 Gm614 uncharacterized protein CXorf65 homolog 1 20,4 3 4 1 16,8 3 5 1 23 4 4 1 29,8 5 17 0,000611 85701985 Gm1060 dynein regulatory complex protein 1 1 10,8 8 12 0,9988 2,7 2 3 1 9 7 7 1 12 9 22 0,000246 85702063 1700071K01Rik prohibitin-like 1 32,1 7 4 1 36,9 8 8 1 44,3 9 9 1 47,6 10 21 0,000537 87080819 Slco6b1 solute carrier organic anion transporter family, member 6b1 1 7,9 4 4 - 1,3 1 1 0,9997 5,2 2 2 1 13,1 6 7 0,000143 87162476 ccdc108 coiled-coil domain-containing protein 108 1 1,5 3 3 - 0 0 0 1 1,5 3 3 1 2,5 5 7 0,0000502 87239970 lipe hormone-sensitive lipase isoform 1 1 7,1 4 6 1 12,1 7 11 1 16,6 10 10 1 17,9 10 42 0,000538 88196767 MLF1 myeloid leukemia factor 1 isoform a 1 9,7 3 6 1 10,1 3 6 1 27 7 7 1 30,7 8 25 0,000698 89886473 9030607L17Rik izumo sperm-egg fusion protein 4 precursor 1 34,8 8 42 1 39,6 8 24 1 37,9 9 9 1 40,1 9 107 0,00336115 9055178 Gm8219 mitochondrial pyruvate carrier 1 1 21,1 3 3 - 0 0 0 0,999 14,7 2 2 0,9999 21,1 3 6 0,000546 phospholipid hydroperoxide glutathione peroxidase, nuclear 90903233 Gpx4 isoform 1 1 38,6 7 38 1 26,4 5 28 1 48,7 10 10 1 48,2 9 147 0,00344686 91206392 MPI mannose-6-phosphate isomerase 1 7,6 2 3 1 7,6 2 4 1 25,3 7 7 1 25,3 7 17 0,000418 91206446 Gm14316 serine protease inhibitor, Kunitz type 5 precursor 1 20 2 3 - 0 0 0 - 0 0 0 1 20 2 4 0,000288 9256624 PGAM2 phosphoglycerate mutase 2 1 53 13 25 1 38,3 10 48 1 51 14 14 1 53,4 15 120 0,0040415 94536844 rpiA ribose-5-phosphate isomerase 1 11,6 3 3 - 4,3 1 2 1 11,6 3 3 1 15,8 4 9 0,000227 9506589 Fbp1 fructose-1,6-bisphosphatase 1 1 31,1 7 12 1 21,9 5 8 1 42,6 13 13 1 40,2 11 34 0,000906 9506739 nagK N-acetyl-D-glucosamine kinase isoform 1 1 16,6 4 5 1 12,2 3 7 1 16,9 5 5 1 25,7 7 18 0,00055 95772156 1700019N12Rik testis-expressed sequence 40 protein 1 26,8 5 9 0,999 12,4 2 4 1 17,5 3 3 1 26,8 5 19 0,000984 9789881 Arl3 ADP-ribosylation factor-like protein 3 1 36,8 5 6 - 0 0 0 1 28 4 4 1 36,8 5 12 0,000512 LETM1 and EF-hand domain-containing protein 1, mitochondrial 9789997 LETM1 precursor 1 20,3 10 11 1 10,3 6 6 1 29,5 15 15 1 27,9 14 31 0,000367 9790051 pfkp 6-phosphofructokinase type C 1 38,4 21 33 1 15,3 10 21 1 40,8 24 24 1 43,8 26 103 0,00137501 9790055 Gm2199 mitochondrial carrier homolog 2 1 25,4 6 21 1 10,9 3 6 1 32,3 8 8 1 32,3 8 63 0,00232098 9790123 NME4 nucleoside diphosphate kinase, mitochondrial precursor 1 25,8 4 5 1 32,3 5 7 1 35,5 5 5 1 39,8 6 20 0,00117722 9790131 Slc22a21 solute carrier family 22 member 21 1 5,3 2 4 1 5,3 2 4 1 8,9 4 4 1 7,4 3 15 0,000461 9910416 DNAJB8 dnaJ homolog subfamily B member 8 1 18,9 3 4 - 4,4 1 3 1 22,5 4 4 1 27,8 4 11 0,000421 9910582 Tex101 testis-expressed sequence 101 protein precursor 1 36 7 15 1 28 5 9 1 30,4 6 6 1 36 7 55 0,00254418 118130874 1700001P01Rik uncharacterized protein C17orf98 homolog 0,9999 9,7 2 4 1 24,5 3 5 1 33,5 3 3 1 24,5 3 14 0,00077 124487327 ARMC3 armadillo repeat-containing protein 3 isoform 1 0,9999 2,8 2 3 0,9988 3,9 2 2 1 20,6 15 15 1 17,7 12 21 0,000189 149261181 Glipr1l3 PREDICTED: GLIPR1-like protein 1-like 0,9999 16,5 4 9 - 0 0 0 1 16,5 5 5 1 16,5 5 27 0,000967 15011842 Smcp sperm mitochondrial-associated cysteine-rich protein 0,9999 16,2 2 5 - 7,1 1 5 1 23,4 3 3 1 23,4 3 37 0,00361201 163644329 APOE apolipoprotein E precursor 0,9999 7,1 2 2 1 14,8 4 6 1 15,1 4 4 1 22,2 6 13 0,000317 18087731 Dynll2 dynein light chain 2, cytoplasmic 0,9999 49,4 3 4 - 0 0 0 0,9996 58,4 4 4 1 58,4 4 9 0,000876 213688406 Gm11582 dynein light chain 1, cytoplasmic 0,9999 49,4 3 3 0,9999 49,4 3 3 1 58,4 4 4 1 58,4 4 11 0,00109698 407262105 Dnhc12 PREDICTED: dynein heavy chain 12, axonemal 0,9999 1,1 4 3 - 0,2 1 1 1 6 20 20 1 6,7 23 26 0,0000566 6680712 aqp7 aquaporin-7 0,9999 9,2 2 3 - 5,3 1 1 1 11,6 3 3 0,9998 9,2 2 8 0,000465 histidine triad nucleotide-binding protein 2, mitochondrial 110625719 HINT2 precursor 0,9998 21,5 2 4 - 9,2 1 2 1 29,4 3 3 1 29,4 3 9 0,000398 110625843 LRRC34 leucine-rich repeat-containing protein 34 0,9998 6 2 2 - 0 0 0 1 25,1 9 9 1 25,1 9 12 0,000242 112293264 PDIA3 protein disulfide-isomerase A3 precursor 0,9998 4,6 2 3 1 8,5 4 12 1 38,6 16 16 1 37,6 16 37 0,000492 114158691 Cops8 COP9 signalosome complex subunit 8 0,9998 13,9 2 2 - 6,7 1 2 1 30,1 4 4 1 30,1 4 9 0,000541 116734870 LTA4H leukotriene A-4 hydrolase 0,9998 4,4 2 2 - 2 1 1 0,9997 3,6 2 2 1 6,1 3 5 0,000072 117606409 wbp2nl postacrosomal sheath WW domain-binding protein 0,9998 6,1 2 6 0,9995 6,1 2 14 0,9998 6,1 2 2 0,9998 6,1 2 22 0,0008 12963511 Gm9646 40S ribosomal protein S19 0,9998 13,1 2 3 - 0 0 0 1 19,3 3 3 1 19,3 3 6 0,000231 12963569 Ppp1r7 protein phosphatase 1 regulatory subunit 7 0,9998 5,5 2 3 1 13,3 3 3 1 16,9 5 5 1 27,4 7 12 0,00025 145966883 PSMD14 26S proteasome non-ATPase regulatory subunit 14 0,9998 12,6 2 2 0,999 8,7 2 3 0,9998 6,5 2 2 1 19,4 4 7 0,000289 157909797 tomm40 mitochondrial import receptor subunit TOM40 homolog 0,9998 7,5 2 2 - 0 0 0 1 11,6 3 3 1 14,7 4 5 0,000168 176866044 allC probable allantoicase 0,9998 6,5 2 2 - 0 0 0 1 11,1 3 3 1 15 4 5 0,00012 19745150 cyb5r3 NADH-cytochrome b5 reductase 3 0,9998 7 2 2 - 4,3 1 1 0,9998 6,3 2 2 1 10,6 3 5 0,000132 205361112 DNAJA3 dnaJ homolog subfamily A member 3, mitochondrial isoform 1 0,9998 5 2 2 0,9995 6,2 2 2 1 12,1 3 3 1 14,6 4 8 0,000128 20799907 HIST2H3B histone H2A type 2-A 0,9998 14,8 1 4 - 0 0 0 0,9999 20,3 2 2 0,9996 14,8 1 6 0,000395 21489937 lancl1 lanC-like protein 1 0,9998 5,8 2 2 - 2,8 1 1 1 7,8 3 3 1 7,8 3 9 0,000247 21746161 Tubb2a-ps2 tubulin beta-2B chain 0,9998 58,7 19 6 0,9992 36 12 5 1 65,2 21 21 1 61,3 20 23 0,000314 254675236 KIF9 kinesin-like protein KIF9 isoform 2 0,9998 2,9 2 2 1 8,2 5 6 1 6,2 4 4 1 8,2 5 11 0,00013 256000799 Gm5617 uncharacterized protein C11orf71 homolog 0,9998 22,2 2 2 1 30,8 3 3 - 12,8 1 1 1 30,8 3 6 0,000401 284172505 1700007I08Rik actin-like protein 10 0,9998 5,8 1 2 - 0 0 0 1 13,6 3 3 1 13,6 3 8 0,000233 29244434 BPI bactericidal permeability-increasing protein precursor 0,9998 5,3 2 2 - 2,3 1 1 1 12,3 4 4 1 12,3 4 7 0,000157 31560116 1110032A03Rik UPF0686 protein C11orf1 homolog 0,9998 12,7 2 3 - 6 1 2 0,9999 12,7 2 2 0,9998 12,7 2 10 0,000655 31981468 IMMP2L mitochondrial inner membrane protease subunit 2 0,9998 11,4 2 6 1 16,6 3 7 1 16,6 3 3 1 32 4 18 0,000735 PREDICTED: orphan sodium- and chloride-dependent 377834273 Ntt5 neurotransmitter transporter NTT5 0,9998 3,3 2 2 - 0 0 0 1 5,4 3 3 0,9998 3,3 2 4 0,000115 58037161 LYZL6 lysozyme-like protein 6 precursor 0,9998 12,8 1 3 0,9988 12,8 1 3 1 18,9 2 2 1 18,9 2 12 0,00164241 Réplica 1 Réplica 2 Réplica 3 Datos combinados

9506911 NDUFA1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 0,9998 14,3 1 3 0,9991 14,3 1 2 - 14,3 1 1 0,9997 14,3 1 6 0,000593 110625690 RECQL ATP-dependent DNA helicase Q1 isoform 1 0,9997 3,5 2 2 - 0 0 0 - 1,7 1 1 0 0 2 0 - 113931146 1700019L03Rik uncharacterized protein C9orf117 homolog 0,9997 2,9 1 3 - 0 0 0 0,9998 4,6 2 2 1 4,6 2 5 0,0000806 116063531 CAR1 carbonic anhydrase 1 0,9997 9,2 2 2 1 26,1 5 5 1 52,5 10 10 1 52,5 10 20 0,000787 119392079 LOC100044248 uncharacterized protein C12orf60 homolog 0,9997 16,6 2 2 - 0 0 0 1 13,8 3 3 1 24,7 4 6 0,000183 121674797 PPT1 palmitoyl-protein thioesterase 1 precursor 0,9997 10,8 2 2 - 4,9 1 1 0,9999 10,8 2 2 0,9998 10,8 2 6 0,00022 12963687 SSNA1 Sjoegren syndrome nuclear autoantigen 1 homolog 0,9997 20,2 2 2 - 0 0 0 1 33,6 3 3 1 33,6 3 5 0,000293 13277354 ubxn6 UBX domain-containing protein 6 0,9997 5 2 2 - 0 0 0 - 2 1 1 0,9997 5 2 3 0,0000588 13385384 PSMD12 26S proteasome non-ATPase regulatory subunit 12 0,9997 4,4 2 2 0,9993 3,7 2 2 1 17,1 7 7 1 17,1 7 11 0,000186 15011841 gpX3 glutathione peroxidase 3 precursor 0,9997 18,1 3 2 - 0 0 0 1 21,7 4 4 1 21,7 4 5 0,0002 154090941 Krt42 keratin, type I cytoskeletal 42 0,9997 10,2 5 2 - 6,2 3 1 - 0 0 0 0,9997 10,2 5 3 0,0000642 21312872 LRP2BP LRP2-binding protein 0,9997 7,5 2 2 - 0 0 0 1 11,3 3 3 1 10,7 3 6 0,000153 21313516 Gm15683 cytochrome c oxidase protein 20 homolog 0,9997 21,4 2 2 - 0 0 0 - 0 0 0 0,9997 21,4 2 3 0,000253 226958374 Sypl synaptophysin-like protein 1 isoform 1 0,9997 8,6 2 5 - 10,3 1 2 0,9994 14 2 2 1 18,9 3 15 0,000793 237649068 Wfdc6b WAP four-disulfide core domain protein 6B precursor 0,9997 10,1 2 2 - 0 0 0 - 6,8 1 1 1 16,9 3 4 0,000244 251823722 1700023D19Rik izumo sperm-egg fusion protein 2 precursor 0,9997 11,4 2 5 1 21,2 4 5 1 16,6 3 3 1 21,2 4 14 0,000686 254587947 ppp2r4 serine/threonine-protein phosphatase 2A activator 0,9997 7,1 2 2 1 11,1 3 3 1 16,4 4 4 1 20,4 5 10 0,00025 electron transfer flavoprotein-ubiquinone oxidoreductase, 254588014 Etfdh mitochondrial precursor 0,9997 3,7 2 2 0,9986 3,7 2 3 1 18,3 9 9 1 18,3 9 15 0,000212 294459930 Gpr64 epididymis-specific protein isoform 1 precursor 0,9997 12,7 2 6 0,9989 12,7 2 3 0,9998 12,7 2 2 0,9997 12,7 2 16 0,000622 30350218 CATSPER3 cation channel sperm-associated protein 3 isoform 2 0,9997 6,7 2 2 - 0 0 0 - 4,4 1 1 0,9996 6,7 2 3 0,000131 30424589 dusp18 dual specificity protein phosphatase 18 0,9997 5,9 1 2 0,9989 5,9 1 4 - 0 0 0 0 0 1 0 - putative transferase CAF17 homolog, mitochondrial isoform 1 30424663 A230051G13Rik precursor 0,9997 7 2 2 0,9988 7 2 2 0,9996 7 2 2 0,9996 7 2 6 0,000171 30841008 RAB18 ras-related protein Rab-18 isoform 2 precursor 0,9997 10,7 2 3 0,9992 11,7 2 3 1 35,4 6 6 1 35,4 6 12 0,000336 31981100 Gm6204 40S ribosomal protein S14 0,9997 15,9 2 2 - 8,6 1 2 0,9999 15,9 2 2 0,9998 15,9 2 8 0,000423 31981890 B2M beta-2-microglobulin precursor 0,9997 16 2 2 - 0 0 0 0,9997 16 2 2 0,9996 16 2 5 0,000441 NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, 56711244 NDUFS6 mitochondrial precursor 0,9997 31,9 2 2 0,998 31,9 2 2 1 41,4 3 3 1 41,4 3 7 0,000375 6753624 Defb2 beta-defensin 2 precursor 0,9997 39,4 2 2 - 0 0 0 - 0 0 0 0,9996 39,4 2 4 0,000821 84871978 MYCBP C-Myc-binding protein 0,9997 28,2 2 2 - 0 0 0 1 54,4 4 4 1 54,4 4 7 0,000456 94721328 vapa vesicle-associated membrane protein-associated protein A 0,9997 8 2 3 - 0 0 0 0,9998 10,4 2 2 1 13,7 3 8 0,000322 9790191 Gm9840 E3 ubiquitin-protein ligase RBX1 0,9997 24,1 2 2 0,9988 24,1 2 2 0,9997 24,1 2 2 0,9996 24,1 2 6 0,000626 10946854 Gm5081 ribose-phosphate pyrophosphokinase 1 0,9996 9,1 2 2 - 5,3 1 1 - 3,8 1 1 0,9995 9,1 2 4 0,000106 125660458 SLC30A9 zinc transporter 9 0,9996 3,4 2 3 - 2,1 1 1 1 6,7 4 4 1 6,7 4 8 0,000127 [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, 148277602 Pdp1 mitochondrial isoform b 0,9996 5,9 2 2 0,9986 4,5 2 3 0,9997 4,5 2 2 1 11,9 5 8 0,000139 162417975 IDH1 isocitrate dehydrogenase [NADP] cytoplasmic 0,9996 4,8 2 2 1 5,1 2 6 1 14,5 5 5 1 16,7 6 14 0,000245 16716569 PRSS1 protease, serine, 1 precursor 0,9996 8,1 1 12 0,9989 8,1 1 16 0,9997 8,1 1 1 0,9996 8,1 1 39 0,0024237 194353962 Gm8230 ADP-ribosylation factor 1 0,9996 13,3 2 2 - 0 0 0 1 53,6 7 7 1 53,6 7 11 0,000489

19526759 SPATA19 spermatogenesis-associated protein 19, mitochondrial precursor 0,9996 20,1 2 5 1 20,1 2 6 1 31,2 3 3 1 31,2 3 22 0,00122386 21312181 LYZL4 lysozyme-like protein 4 precursor 0,9996 22,8 2 2 - 6,9 1 1 1 40 4 4 1 40 4 8 0,000489 21312812 STARD6 stAR-related lipid transfer protein 6 0,9996 14,2 2 2 - 0 0 0 1 23,2 3 3 1 26,6 4 6 0,000215 21314830 REEP6 receptor expression-enhancing protein 6 isoform 1 0,9996 10,4 2 3 - 0 0 0 1 15,4 3 3 1 15,4 3 9 0,000605

22550102 PLCZ1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 0,9996 4,2 2 2 1 6,3 3 3 1 7,6 3 3 1 12,1 5 8 0,000147 226874906 YWHAE 14-3-3 protein epsilon 0,9996 12,5 3 2 - 3,5 1 1 1 25,9 6 6 1 30,6 7 8 0,000209 229093139 HTRA1 serine protease HTRA1 precursor 0,9996 5,4 2 2 - 2,9 1 6 1 12,9 4 4 1 14,8 5 17 0,000328 G-protein coupled receptor family C group 5 member B isoform 2 23956118 Gprc5b precursor 0,9996 5,9 2 2 - 0 0 0 - 2,9 1 1 0,9992 5,9 2 3 0,000185 240120058 4930544G11Rik ras homolog gene family, member A-like 0,9996 11,4 2 3 - 0 0 0 0,9985 24,9 4 4 0,9921 24,9 4 6 0,000269

254675168 Gm10171 mitochondrial import inner membrane translocase subunit Tim23 0,9996 12,9 2 3 0,9989 12,9 2 2 0,9996 12,9 2 2 1 12,9 2 8 0,000486 312222726 Angel1 protein angel homolog 2 isoform 2 0,9996 4 2 2 - 0 0 0 1 12,6 5 5 1 14,4 6 9 0,000158 319655563 Ccdc136 coiled-coil domain-containing protein 136 isoform 2 0,9996 2,8 2 2 - 0 0 0 1 6,8 5 5 1 7,4 5 8 0,000083 31980844 dhrs1 dehydrogenase/reductase SDR family member 1 0,9996 7 2 2 0,9991 7 2 3 1 10,5 3 3 1 16,3 5 10 0,000227 32469507 Cd59b CD59B glycoprotein precursor 0,9996 16,3 2 2 0,9989 16,3 2 2 0,9994 16,3 2 2 0,9996 16,3 2 7 0,000319 58037419 1700018L24Rik testis-expressed sequence 29 protein 0,9996 11,8 2 4 0,999 11,8 2 10 1 26,3 4 4 1 18,8 3 23 0,00116219 6680019 GCLM glutamate--cysteine ligase regulatory subunit 0,9996 10,2 2 2 - 0 0 0 0,9999 10,2 2 2 0,9998 10,2 2 4 0,000206 76253942 cetn1 centrin-1 0,9996 17,4 2 2 - 0 0 0 1 23,3 3 3 1 22,7 3 5 0,000223 113462019 LOC100047516 casein kinase I isoform gamma-3 0,9995 5,1 2 2 - 0 0 0 1 8,4 3 3 0 0 4 0 - 13385408 rpl11 60S ribosomal protein L11 0,9995 12,9 2 3 - 7,9 1 1 - 5,1 1 1 0,9995 12,9 2 6 0,000249 149363634 ptrh2 peptidyl-tRNA hydrolase 2, mitochondrial isoform b 0,9995 16 2 3 - 0 0 0 - 8,3 1 1 0,9993 16 2 3 0,00013 158854020 echdc1 ethylmalonyl-CoA decarboxylase isoform a 0,9995 7,7 2 2 0,9995 7,4 2 2 1 13,7 4 4 1 11 3 7 0,00018 161086957 4932412H11Rik leucine-rich repeat and death domain-containing protein 1 0,9995 2,8 2 2 - 1,5 1 2 1 10,9 7 7 1 9,5 6 11 0,00013 Réplica 1 Réplica 2 Réplica 3 Datos combinados

161484668 PPP1CB serine/threonine-protein phosphatase PP1-beta catalytic subunit 0,9995 22,9 6 8 1 19 5 8 0,9992 29,7 8 8 0,9776 25,7 7 3 - 162329596 PPIL6 peptidyl-prolyl cis-trans isomerase-like 6 0,9995 9,9 2 2 0,9995 10,9 2 3 1 31,3 8 8 1 26,2 6 10 0,000381 19705424 psmd3 26S proteasome non-ATPase regulatory subunit 3 0,9995 5,7 2 2 - 0 0 0 1 12,1 5 5 1 10 4 7 0,0000975 21312550 SLC25A35 solute carrier family 25 member 35 0,9995 8,3 2 2 - 0 0 0 - 0 0 0 0,9994 8,3 2 4 0,000111 21314844 CATSPER1 cation channel sperm-associated protein 1 0,9995 1,9 1 2 0,9994 3,2 2 2 0,9999 1,9 1 1 1 3,2 2 7 0,000211 254675124 Ccdc19 nasopharyngeal epithelium specific protein 1 0,9995 4,2 2 3 0,9989 3,6 2 5 1 12 5 5 1 13,6 6 29 0,000362 254692940 TTC18 tetratricopeptide repeat domain 18 isoform 3 0,9995 1,5 2 2 - 1,2 1 2 1 8,2 8 8 1 8,7 8 17 0,000167 255003729 EFHB EF-hand domain-containing family member B 0,9995 2,9 2 2 - 0 0 0 1 14,5 10 10 1 11,6 7 12 0,000124 307691260 LOC627607 uncharacterized protein LOC627607 0,9995 4,3 2 2 0,9991 5,7 2 3 1 6,8 3 3 1 7,6 3 6 0,000107 33504483 rps9 40S ribosomal protein S9 0,9995 8,8 2 2 - 4,6 1 3 0,9992 8,8 2 2 1 12,9 3 7 0,000235 54607171 KRT6A keratin, type II cytoskeletal 6A 0,9995 9,9 5 2 - 0 0 0 - 0 0 0 0,9991 12,8 7 2 0,0000365 58037521 CST11 cystatin-11 precursor 0,9995 17,3 2 2 - 8,6 1 1 0,9998 14,4 2 2 1 23 3 5 0,000249 6681095 Gm10108 cytochrome c, somatic 0,9995 23,8 2 2 - 0 0 0 1 49,5 5 5 1 49,5 5 9 0,000517 85701967 CCDC27 coiled-coil domain-containing protein 27 0,9995 3,3 2 2 1 6,9 4 7 1 8,1 4 4 1 11,7 6 16 0,00018 110625761 LOC100048880 AFG3-like protein 2 0,9994 2 2 2 0,9988 2,4 2 2 1 17,3 11 11 1 17,3 11 16 0,000147 11230802 actn4 alpha-actinin-4 0,9994 2,2 2 2 - 0 0 0 - 0 0 0 0,9993 2,2 2 3 0,0000256 116268115 AUH methylglutaconyl-CoA hydratase, mitochondrial precursor 0,9994 9,2 2 2 - 2,9 1 2 1 21 5 5 1 21 5 9 0,000194 13385084 NIPSNAP3B protein NipSnap homolog 3B 0,9994 8,9 2 2 1 12,1 2 3 1 18,2 3 3 1 25,1 4 8 0,000298

149274885 LOC100046684 PREDICTED: ES1 protein homolog, mitochondrial-like isoform 1 0,9994 6,8 2 3 - 0 0 0 1 39,8 7 7 1 41 7 14 0,000403 227500281 etfA electron transfer flavoprotein subunit alpha, mitochondrial 0,9994 9 2 2 1 15 3 3 1 28,5 7 7 1 34,5 8 12 0,000258 254675164 Tmem190 transmembrane protein 190 precursor 0,9994 15,1 2 5 1 15,1 2 14 1 23,5 3 3 1 15,1 2 30 0,00283156 NFU1 iron-sulfur cluster scaffold homolog, mitochondrial isoform 282154801 Gm7859 2 precursor 0,9994 10,6 2 2 - 5,9 1 1 0,9995 10,6 2 2 0,9992 10,6 2 5 0,000198 294610650 Gstt4 glutathione S-transferase theta-4 0,9994 10 2 2 - 6,2 1 1 1 12,9 3 3 0,9994 10 2 6 0,000217 29789199 LOC100044315 importin-5 0,9994 2,1 2 2 - 0 0 0 1 8,2 7 7 1 7,4 6 8 0,000083 307691211 - regulator of G-protein signaling 22 0,9994 1,4 2 2 - 0 0 0 1 2,1 3 3 1 2,7 4 7 0,0000539 377834275 LOC100862214 PREDICTED: uncharacterized protein LOC100862214 0,9994 8,7 2 3 - 0 0 0 1 23,5 6 6 1 23,5 6 13 0,000397 50979268 1700065I17Rik uncharacterized protein C5orf48 homolog 0,9994 16,3 2 3 0,9992 13,5 2 3 1 27,7 4 4 1 27,7 4 18 0,00133879 6679803 FKBP1A peptidyl-prolyl cis-trans isomerase FKBP1A 0,9994 25 2 2 0,9989 13 1 2 1 25 2 2 1 25 2 7 0,000508 66841374 1700007G11Rik uncharacterized protein C4orf22 homolog 0,9994 6,9 2 3 - 3,4 1 1 1 9,9 3 3 1 10,3 3 8 0,000346 95113671 PMPCB mitochondrial-processing peptidase subunit beta precursor 0,9994 3,7 2 2 0,999 4,5 2 4 1 6,1 3 3 1 6,1 3 9 0,000153 9789907 STUB1 STIP1 homology and U box-containing protein 1 0,9994 7,2 2 2 - 0 0 0 - 0 0 0 1 10,2 3 3 0,0000598 9910550 SEC11A signal peptidase complex catalytic subunit SEC11A 0,9994 9,5 2 4 - 4,5 1 3 0,9996 9,5 2 2 1 13,4 3 11 0,000418 13386112 H2afb1 histone H2A-Bbd type 1 0,9993 18,9 2 2 - 11,7 1 1 0,9995 19,8 2 2 0,9995 18,9 2 5 0,000351 21312758 PPP6C serine/threonine-protein phosphatase 6 catalytic subunit 0,9993 10,5 2 2 - 6,2 1 1 1 14,4 4 4 1 20,7 5 8 0,000203 244790085 CCDC81 coiled-coil domain-containing protein 81 0,9993 3,8 2 3 1 5,4 3 5 1 11,5 6 6 1 9 5 18 0,000201 24762230 Gm14166 40S ribosomal protein S15a 0,9993 16,2 2 2 - 0 0 0 - 0 0 0 0,9988 16,2 2 3 0,000151 255003735 Gm15451 60S ribosomal protein L10a 0,9993 9,2 2 2 - 0 0 0 0,9996 9,7 2 2 1 9,7 3 4 0,000154 94394748 1700016G14Rik PREDICTED: uncharacterized protein LOC666145 0,9993 8,8 2 2 - 0 0 0 0,9991 9,7 2 2 0,9987 8,8 2 3 0,000141 9845257 Hist1h1c histone H1.2 0,9993 12,7 2 2 - 0 0 0 1 22,6 4 4 0,9995 12,7 2 4 0,000167 120407060 Antxrl anthrax toxin receptor-like precursor 0,9992 3,3 2 2 - 0 0 0 1 7,6 4 4 1 6,4 3 5 0,0000919 149260690 LOC100045901 PREDICTED: ubiquitin-conjugating enzyme E2 L3-like 0,9992 20,1 2 2 - 0 0 0 - 0 0 0 0,9993 20,1 2 3 0,000174 156713423 PSMD8 26S proteasome non-ATPase regulatory subunit 8 0,9992 4,5 2 2 - 2,8 1 1 1 11 4 4 1 11,9 4 7 0,000184 238550131 Efcab9 EF-hand calcium-binding domain-containing protein 9 0,9992 9,7 2 2 - 0 0 0 0,9996 16,2 2 2 1 21,8 3 5 0,000247 34328471 RALA ras-related protein Ral-A precursor 0,9992 10,2 2 2 - 6,8 1 1 - 0 0 0 0,9993 10,2 2 3 0,00012 56797739 MLYCD malonyl-CoA decarboxylase, mitochondrial 0,9992 6,9 2 2 - 2,2 1 1 - 0 0 0 0,9994 6,9 2 3 0,0000482 158749545 SLC25A40 solute carrier family 25 member 40 0,9991 5 2 3 - 0 0 0 - 3 1 1 0,9991 5 2 5 0,000133 26080429 Aldh16a1 aldehyde dehydrogenase family 16 member A1 0,9991 2,9 2 2 0,9995 3,1 2 2 1 4,9 3 3 1 8 5 8 0,000132 27754007 NDUFAB1 acyl carrier protein, mitochondrial precursor 0,9991 15,4 2 2 - 0 0 0 - 5,8 1 1 0,9988 15,4 2 3 0,000202 76781472 Gm711 probable inactive protein kinase-like protein SgK071 homolog 0,9991 2,6 2 2 - 0 0 0 1 8,2 5 5 1 8,2 5 8 0,000177 7949005 LOC674583 ATP synthase-coupling factor 6, mitochondrial precursor 0,9991 25,9 2 2 - 17,6 1 1 0,9996 25,9 2 2 1 25,9 2 5 0,000291 113930767 Wfdc15b WAP four-disulfide core domain protein 15B precursor 0,999 27,5 2 2 - 0 0 0 - 16,2 1 1 0,9985 27,5 2 3 0,00026 225690614 ACOT7 cytosolic acyl coenzyme A thioester hydrolase isoform 2 0,999 6,6 2 2 - 0 0 0 0,9996 6,6 2 2 1 9,7 3 5 0,000134 28916703 Prdx6-rs1 peroxiredoxin 6, related sequence 1 0,999 23,2 4 2 - 5,4 1 1 1 32,6 5 5 1 32,6 5 5 0,000133 61656190 Lrrc52 leucine-rich repeat-containing protein 52 precursor 0,999 6,7 2 2 - 0 0 0 - 0 0 0 0,9988 6,7 2 2 0,0000917 112734843 Tssk2 testis-specific serine/threonine-protein kinase 2 0,9989 6,7 3 2 - 2,5 1 1 0,9999 13,7 4 4 1 15,9 6 6 0,000123 12963675 ociad1 OCIA domain-containing protein 1 isoform 1 0,9989 7,7 2 2 0,9987 7,7 2 2 0,9995 17,5 2 2 1 16,6 3 6 0,000218 158966704 LOC100047501 40S ribosomal protein S16 0,9989 12,3 2 4 - 0 0 0 0,9991 12,3 2 2 0,9991 12,3 2 6 0,000244 27370404 CCDC113 coiled-coil domain-containing protein 113 0,9989 7,4 2 2 - 0 0 0 0,9997 5,3 2 2 1 11,9 4 5 0,0000972 PREDICTED: mitochondrial import inner membrane translocase 309268906 Tim14 subunit TIM14-like 0,9989 15,5 2 2 0,9991 19,8 2 2 - 12,1 1 1 1 27,6 3 5 0,000274 58037191 RAB28 ras-related protein Rab-28 0,9989 14 2 2 - 0 0 0 0,9999 14 2 2 0,9997 14 2 4 0,000116 85702045 LOC100048051 multidrug and toxin extrusion protein 2 0,9989 2,8 2 2 - 0 0 0 - 2,4 1 1 1 5,2 3 3 0,000096 161760667 lmna prelamin-A/C isoform C 0,9988 3,7 2 2 1 13,6 7 17 1 12,2 7 7 1 18,3 10 28 0,000318 21362321 RNF126 RING finger protein 126 0,9988 6,1 2 2 - 2,9 1 1 - 0 0 0 0,9988 8,3 2 3 0,000156 31543918 ube2v2 ubiquitin-conjugating enzyme E2 variant 2 isoform 1 0,9988 13,1 2 3 - 0 0 0 - 0 0 0 0 0 4 0 - 6752944 Abcg2 ATP-binding cassette sub-family G member 2 0,9988 3 2 2 - 0 0 0 0,9994 3,3 2 2 1 4,9 3 3 0,0000604 27229278 AGPAT3 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma 0,9987 5,1 2 2 - 0 0 0 1 15,4 5 5 1 15,4 5 7 0,000193 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 282398108 RPN1 subunit 1 precursor 0,9987 3,3 2 2 0,9991 3,9 2 2 1 24,3 11 11 1 24,7 11 18 0,000232 Réplica 1 Réplica 2 Réplica 3 Datos combinados

84781779 NDUFB6 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 0,998 19,5 2 2 - 14,1 1 2 1 17,2 3 3 1 32 5 9 0,000514 94366539 Gm13570 PREDICTED: mitochondrial pyruvate carrier-like protein-like 0,998 44,3 4 10 - 0 0 0 - 13,9 2 2 0,9979 44,3 4 23 0,00173952 CDP-diacylglycerol--glycerol-3-phosphate 3- 110626163 PGS1 phosphatidyltransferase, mitochondrial 0,9979 5,1 2 2 0,9991 5,1 2 2 - 0 0 0 0,9996 5,1 2 3 0,000057 14780884 POLDIP2 polymerase delta-interacting protein 2 0,9979 7,6 2 2 0,9989 7,6 2 2 1 13,6 4 4 1 13,6 4 5 0,000107 7305339 Odf2 outer dense fiber protein 2 isoform c 0,996 45,6 27 6 - 0 0 0 - 48,7 29 29 0,9819 49,8 30 6 - 83999999 Gnpda2 glucosamine-6-phosphate isomerase 2 0,9945 10,1 2 2 - 0 0 0 1 13,4 3 3 1 17,8 4 5 0,000136 6680924 CFL1 cofilin-1 0,993 13,9 2 4 - 13,9 2 1 - 0 0 0 0,9848 13,9 2 3 - 20911031 KRT5 keratin, type II cytoskeletal 5 0,9924 5,7 3 15 0,9994 8,6 5 5 0,9997 7,2 4 4 0,9826 10 6 7 - 295054193 Odf2 outer dense fiber protein 2 isoform e 0,992 42,2 27 4 1 31,7 19 76 1 48,1 31 31 1 47,9 31 559 0,00435 114145561 Gm5604 keratin, type II cytoskeletal 8 - 0 0 0 1 13,5 6 12 1 10,6 5 5 1 20,4 10 26 0,000408 124301188 4732415M23Rik uncharacterized protein C16orf55 homolog - 7,6 1 2 1 31,8 3 5 0,9998 18,2 2 2 1 31,8 3 10 0,00062 13385518 Arl8b ADP-ribosylation factor-like protein 8B - 3,8 1 1 1 18,8 4 5 1 14 3 3 1 18,8 4 9 0,000351 147905740 MUC5B mucin 5, subtype B, tracheobronchial precursor - 0 0 0 1 3,9 7 8 - 0 0 0 1 4,1 8 11 0,0000354 160415213 RHOC rho-related GTP-binding protein RhoC precursor - 0 0 0 1 13 2 4 - 23,3 4 4 0,9999 23,3 4 10 0,000403 163310765 alb serum albumin precursor - 2,1 1 15 1 15,8 8 13 1 31,4 17 17 1 37,8 20 65 0,000737 19527224 CSNK1G2 casein kinase I isoform gamma-2 isoform 1 - 0 0 0 1 6 2 3 - 0 0 0 1 14,5 5 8 0,000175 198278494 4933433C11Rik uncharacterized protein LOC74472 - 2,6 1 2 1 12,1 3 4 - 0 0 0 1 16 4 8 0,000319 227496048 Gm5347 a disintegrin and metallopeptidase domain 34 - 1,7 1 2 1 4,8 3 4 1 9,5 6 6 1 11,2 7 12 0,000214 227500026 LOC100047179 protein-glutamine gamma-glutamyltransferase 4 - 0 0 0 1 6,4 4 9 - 0 0 0 1 8,4 5 11 0,000159 254540027 Mdh1 malate dehydrogenase, cytoplasmic - 3 1 1 1 9,3 3 3 1 22,5 6 6 1 28,7 8 12 0,000287 254540166 HSPA5 78 kDa glucose-regulated protein precursor - 6,3 3 1 1 18,3 10 10 1 33,4 17 17 1 35 19 31 0,000368 257153450 Semg1 semenogelin I precursor - 0 0 0 1 12,3 4 12 - 0 0 0 1 12,3 4 12 0,000234 257467625 FCGBP Fc fragment of IgG binding protein-like precursor - 0 0 0 1 14,3 28 61 - 0 0 0 1 16,3 32 91 0,000459 27734998 Tmem11 transmembrane protein 11, mitochondrial isoform 1 - 4,5 1 1 1 14,2 2 3 0,9995 9,7 2 2 1 18,8 3 6 0,000342 282721066 4931409K22Rik IQ and AAA domain-containing protein 1-like - 1,5 1 4 1 5,5 4 5 1 5,6 4 4 1 6,9 5 17 0,000147 monofunctional C1-tetrahydrofolate synthase, mitochondrial 283135110 MTHFD1L precursor - 0 0 0 1 8,3 6 6 1 7,9 6 6 1 14,5 11 15 0,000125 312222784 Vdac3 voltage-dependent anion-selective channel protein 3 isoform 1 - 0 0 0 1 25,4 5 10 - 0 0 0 0 0 9 0 - 31560090 PLBD2 putative phospholipase B-like 2 precursor - 2 1 1 1 5,7 3 4 1 11,8 6 6 1 14,8 7 10 0,0002 31980806 PGRMC1 membrane-associated progesterone receptor component 1 - 4,1 1 1 1 19 3 3 1 21,5 3 3 1 26,2 4 12 0,000452 31982133 AMDHD2 putative N-acetylglucosamine-6-phosphate deacetylase - 3,4 1 1 1 10,3 3 3 1 16,6 5 5 1 20,8 6 9 0,000237 356582477 Ogdh 2-oxoglutarate dehydrogenase, mitochondrial isoform 1 - 45,6 36 1 1 24,2 17 36 - 0 0 0 1 52,5 41 184 0,0018471 358439483 Immt mitochondrial inner membrane protein isoform 2 - 59,8 38 12 1 40,8 25 72 1 57,6 38 38 0 0 40 0 - 37700232 Nme1 nucleoside diphosphate kinase A - 0 0 0 1 31,6 4 5 1 56,6 7 7 1 56,6 7 13 0,000743 42741690 Gm7181 ubiquitin-conjugating enzyme E2 variant 1 - 0 0 0 1 30,6 4 4 1 38,1 4 4 1 44,9 5 12 0,000539 6680606 KRT19 keratin, type I cytoskeletal 19 - 0 0 0 1 25,3 9 12 1 35,7 13 13 1 42,2 15 24 0,000362 67972647 arsA arylsulfatase A precursor - 3 1 1 1 6,9 3 3 1 18,2 6 6 1 20,4 7 12 0,000423 6996913 LOC100048867 annexin A2 - 0 0 0 1 10,9 3 3 1 30,4 9 9 1 33,3 10 12 0,000224 84490394 Spnb1 spectrin beta chain, erythrocytic - 0 0 0 1 3,3 6 9 1 13,1 24 24 1 13,1 24 45 0,000146 93102411 GALC galactocerebrosidase precursor - 2,3 1 1 1 5,1 3 3 - 0 0 0 1 5,1 3 4 0,0000883 9789933 NUDT3 diphosphoinositol polyphosphate phosphohydrolase 1 - 8,3 1 1 1 18,5 3 3 - 6 1 1 1 18,5 3 5 0,000262 13385654 4921536K21Rik uncharacterized protein C5orf52 homolog - 7,7 1 1 0,9999 25,6 3 3 1 25,6 4 4 1 38,7 5 10 0,000444 160415215 Akr1b7 aldose reductase-related protein 1 - 0 0 0 0,9999 8,5 3 3 1 22,2 6 6 1 22,2 6 10 0,000279 357933645 Ptgr2 prostaglandin reductase 2 isoform 1 - 5,1 1 1 0,9999 10,5 3 3 1 10,5 3 3 1 10,5 3 7 0,000204 6753912 FTH1 ferritin heavy chain - 7,1 1 1 0,9999 17,6 3 4 1 23,6 4 4 1 23,6 4 10 0,000436 124486624 4932425I24Rik protein MAATS1 - 1,3 1 2 0,9995 3,1 2 3 0,9996 3,8 2 2 1 5,6 3 8 0,0000918 188219589 LMNB1 lamin-B1 - 1,4 1 1 0,9995 6,5 3 8 1 5,6 3 3 1 11,4 6 11 0,000125 21735489 C030006K11Rik UPF0598 protein C8orf82 homolog isoform 1 - 0 0 0 0,9995 13,8 2 2 1 22,9 3 3 1 22,9 3 6 0,000211

262050625 Ssr4 translocon-associated protein subunit delta isoform 1 precursor - 0 0 0 0,9995 14 2 4 1 19,8 3 3 1 19,8 3 10 0,000689 294460012 Aldh1b2 aldehyde dehydrogenase 3 family, member B2 - 4,3 1 1 0,9995 9,2 2 3 - 0 0 0 0,9998 9,2 2 4 0,0000868 31982030 ARHGDIA rho GDP-dissociation inhibitor 1 - 7,4 1 1 0,9995 15,2 2 3 1 30,9 3 3 0,9998 15,2 2 7 0,000301 34850748 MORN2 MORN repeat-containing protein 2 - 11,4 1 1 0,9995 29,1 2 2 - 11,4 1 1 0,9998 29,1 2 5 0,000433 6755152 prep prolyl endopeptidase - 0 0 0 0,9995 4,2 2 2 - 0 0 0 0,9998 4,2 2 2 0,000022 112293266 HSPA4 heat shock 70 kDa protein 4 - 0 0 0 0,9994 3,3 2 2 - 1,5 1 1 1 4,9 3 3 0,000028 148232950 Pgcp carboxypeptidase Q precursor - 2,3 1 2 0,9994 4,7 2 3 1 16 6 6 1 16 6 11 0,000246 247269607 Dguok deoxyguanosine kinase, mitochondrial isoform 1 - 0 0 0 0,9994 9,7 2 2 1 20,6 4 4 1 13 3 5 0,000162 low molecular weight phosphotyrosine protein phosphatase isoform 31542070 acp1 2 - 0 0 0 0,9994 17,1 2 4 0,9996 18,4 2 2 1 24,1 3 7 0,000324 39652626 LOC633860 3'(2'),5'-bisphosphate nucleotidase 1 - 0 0 0 0,9994 7,5 2 2 1 18,2 5 5 1 18,8 5 6 0,000141 40556608 HSP90AB1 heat shock protein HSP 90-beta - 0 0 0 0,9994 8,1 5 2 1 12,2 8 8 1 13,7 9 5 0,0000553 disintegrin and metalloproteinase domain-containing protein 5 440546413 Adam5 isoform 1 precursor - 1,9 1 1 0,9994 4,2 2 3 1 6,9 4 4 1 6,9 4 10 0,000106 68226731 npepps puromycin-sensitive aminopeptidase - 1,3 1 1 0,9994 2,4 2 2 - 0 0 0 1 3,7 3 3 0,00003 13384642 synj2bp synaptojanin-2-binding protein - 9,7 1 1 0,9993 15,9 2 4 0,9996 14,5 2 2 1 24,1 3 7 0,000421 Réplica 1 Réplica 2 Réplica 3 Datos combinados

6679767 fdxr NADPH:adrenodoxin oxidoreductase, mitochondrial precursor - 0 0 0 0,9992 5,5 2 2 - 0 0 0 1 8,1 3 3 0,0000495 110835737 Lcn5 epididymal-specific lipocalin-5 isoform a precursor - 0 0 0 0,9991 14,6 2 2 1 37 5 5 1 37 5 10 0,000446 158508560 COL18A1 collagen alpha-1(XVIII) chain isoform 1 precursor - 1,3 1 1 0,9991 2,1 2 3 1 2,7 3 3 1 4 5 12 0,0001 264681530 - predicted gene, 17727 precursor - 0 0 0 0,9991 15,6 1 2 - 15,6 1 1 0,9996 15,6 1 4 0,000295 27501448 eIF6 eukaryotic translation initiation factor 6 - 4,1 1 1 0,9991 11,4 2 2 1 17,1 3 3 1 17,1 3 7 0,000366 29789229 SPATA17 spermatogenesis-associated protein 17 isoform 1 - 0 0 0 0,9991 5,3 2 2 - 2,4 1 1 0,9997 5,3 2 3 0,0000575 114326466 Gm12164 ferritin light chain 1 - 0 0 0 0,999 7,1 1 2 0,9993 15,8 2 2 1 15,8 2 4 0,000168 disintegrin and metalloproteinase domain-containing protein 24 157012013 Adam24 precursor - 1,7 1 1 0,999 3,4 2 4 1 4,7 3 3 0,9997 3,4 2 7 0,000124 157951602 Wfdc16 WAP four-disulfide core domain 16 - 14,4 1 2 0,999 26,1 2 4 1 26,1 2 2 1 26,1 2 16 0,00231207 170650601 macrod1 O-acetyl-ADP-ribose deacetylase MACROD1 - 3,4 1 1 0,999 10,5 2 3 1 14,6 3 3 1 21,7 4 7 0,000162 19072792 ERP44 endoplasmic reticulum resident protein 44 precursor - 0 0 0 0,999 3 1 2 - 0 0 0 0,9996 3 1 3 0,0000725 21450325 BLVRB flavin reductase (NADPH) - 0 0 0 0,999 13,1 2 3 1 20,9 4 4 1 17,5 3 8 0,000328 254587970 Cst12 cystatin-12 - 12,1 1 1 0,999 12,1 1 3 1 18,4 2 2 1 18,4 2 10 0,000492 13386452 Hist3h2ba histone H2B type 3-A - 0 0 0 0,9989 20,6 2 2 0,9999 20,6 2 2 0 0 2 0 - 214010153 APEH acylamino-acid-releasing enzyme - 1,5 1 1 0,9989 5,3 2 2 1 7,2 4 4 1 12,6 6 7 0,00011 226528838 9530003J23Rik lysozyme C-like precursor - 0 0 0 0,9989 11,9 1 3 - 0 0 0 0,9996 11,9 1 3 0,000145 von Willebrand factor A domain-containing protein 8 isoform 1 226958579 1300010F03Rik precursor - 0 0 0 0,9989 1,9 2 2 - 1,1 1 1 0,9995 1,9 2 3 0,0000121 318043086 LOC73863 uncharacterized protein LOC73863 - 9,9 1 2 0,9989 25,2 2 2 0,9999 25,2 2 2 1 25,2 2 6 0,000419 31981922 LOC100048601 probable threonine protease PRSS50 precursor - 2,3 1 2 0,9989 5,5 2 6 0,9996 5,5 2 2 1 5,5 2 12 0,000373 31982437 nsdhl sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating - 2,8 1 1 0,9989 5,5 2 2 1 14,1 4 4 1 16,6 5 7 0,000213 85701774 Qrich2 glutamine rich 2 - 2,4 1 1 0,9989 4,6 2 4 - 0 0 0 1 6,9 3 7 0,000156 13384870 Tmem126a transmembrane protein 126A - 4,6 1 2 0,9988 8,7 2 6 1 19,9 4 4 1 13,8 3 13 0,000836 227500514 1700080E11Rik uncharacterized protein LOC73532 - 5,3 1 2 0,9988 10,2 2 2 0,9996 10,2 2 2 0,9995 10,2 2 5 0,000172 244790071 Lrrc51 leucine-rich repeat-containing protein 51 isoform 1 - 5,7 1 2 0,9988 10,4 2 5 1 26,6 4 4 1 26,6 4 13 0,000606 6755154 CIB1 calcium and integrin-binding protein 1 - 0 0 0 0,9988 12,6 2 2 - 4,7 1 1 0,9996 12,6 2 3 0,000107 85701842 Gm216 uncharacterized protein C2orf62 homolog - 2,6 1 1 0,9988 2,6 1 2 1 9,6 4 4 1 7 3 6 0,000155 158937279 mmel1 membrane metallo-endopeptidase-like 1 - 0 0 0 0,9987 2,3 2 2 1 6,5 4 4 1 10,2 6 6 0,0000761 6680988 Gm7795 cytochrome c oxidase subunit 6A1, mitochondrial - 14,3 1 1 0,9987 14,3 1 3 0,9994 14,3 1 1 1 40,2 2 9 0,00149459 7305035 Epb4.2 erythrocyte membrane protein band 4.2 - 0 0 0 0,9987 3,5 2 2 1 8,4 4 4 1 10,1 5 6 0,0000917 16716499 SFXN3 sideroflexin-3 isoform 1 - 0 0 0 0,9986 10,7 2 2 - 5,3 1 1 0,9994 10,7 2 3 0,0000863 326368226 Recql1 ATP-dependent DNA helicase Q1 isoform 3 - 0 0 0 0,9986 4,4 2 2 - 0 0 0 1 6,2 3 5 0,0000665 13385186 CCDC96 coiled-coil domain-containing protein 96 - 2,4 1 1 0,9985 4,8 2 2 - 2,4 1 1 1 12 4 6 0,0000816 34304087 SPINK2 serine protease inhibitor Kazal-type 2 precursor - 16,3 1 1 0,9985 16,3 1 2 - 16,3 1 1 0,9995 16,3 1 4 0,000424 N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase isoform 1 54292135 aga precursor - 0 0 0 0,9985 12,4 2 2 - 0 0 0 0,9994 12,4 2 2 0,0000608 70887605 SPAG16 sperm-associated antigen 16 protein isoform 1 - 0 0 0 0,9985 3,8 2 2 1 12,2 7 7 1 11 6 11 0,000166 9625018 GDE1 glycerophosphodiester phosphodiesterase 1 - 0 0 0 0,9984 7,6 2 2 - 0 0 0 0,9993 7,6 2 2 0,0000617 377834422 Prdx1 PREDICTED: peroxiredoxin-1 - 8 2 4 0,9982 8 2 2 - 0 0 0 0 0 4 0 - 6680722 LOC100046958 ADP-ribosylation factor 5 - 0 0 0 0,9981 15,6 2 3 - 0 0 0 0,9719 38,9 5 1 - 7305185 INSL6 insulin-like peptide INSL6 precursor - 4,2 1 1 0,9981 13,6 2 2 1 15,2 3 3 1 24,6 4 6 0,000252 13385268 CYB5 cytochrome b5 - 15,7 1 1 0,9979 24,6 2 3 1 34,3 3 3 1 24,6 2 8 0,000577 21312298 SHMT2 serine hydroxymethyltransferase, mitochondrial isoform 1 - 0 0 0 0,9979 3,6 2 2 - 2,2 1 1 1 5,8 3 4 0,0000586 28916693 Gsn gelsolin isoform 1 precursor - 0 0 0 0,9979 3 2 2 - 0 0 0 0,9991 3 2 2 0,0000284 58037271 1700023E05Rik testicular haploid expressed gene protein-like - 0 0 0 0,9979 5,9 2 2 1 17,2 6 6 1 13,3 5 10 0,000159 148747558 PRDX2 peroxiredoxin-2 - 5,6 1 1 0,9978 10,1 2 2 1 36,4 6 6 1 36,4 6 20 0,000699 188219614 Uchl1 ubiquitin carboxyl-terminal hydrolase isozyme L1 - 9,4 1 1 0,9975 11,7 2 2 1 16,6 3 3 1 21,1 3 5 0,000208 calcium-binding tyrosine phosphorylation-regulated protein isoform 109255218 Cabyr c - 0 0 0 - 0 0 0 1 31 10 10 1 31 10 3 0,0000929 109948267 4930578I06Rik uncharacterized protein C8orf74 homolog - 3,7 1 1 - 0 0 0 1 19,5 4 4 1 19,5 4 5 0,000159 110625742 1700007K13Rik UPF0691 protein C9orf116 homolog - 5,4 1 2 - 0 0 0 1 44,9 5 5 1 50,3 6 8 0,000549 110625796 4931432M23Rik inactive serine protease 54 precursor - 0 0 0 - 0 0 0 1 10,4 4 4 1 10,4 3 3 0,0000955 110626034 4930519F16Rik uncharacterized protein LOC75106 precursor - 4,3 1 2 - 0 0 0 1 18,6 3 3 0,9998 9,1 2 5 0,000299 113195690 Man2b1 lysosomal alpha-mannosidase precursor - 0 0 0 - 0,9 1 1 1 4,4 3 3 1 4,4 3 4 0,0000457 114326546 pgam1 phosphoglycerate mutase 1 - 0 0 0 - 16,5 4 1 1 37,8 8 8 1 37,8 8 5 0,000175 115430101 C4bp C4b-binding protein precursor - 0 0 0 - 0 0 0 1 9 4 4 1 9 4 4 0,0000855 disintegrin and metalloproteinase domain-containing protein 2 116089327 ADAM2 precursor - 0 0 0 - 2 1 1 1 6,4 3 3 1 6,4 3 5 0,0000713 Réplica 1 Réplica 2 Réplica 3 Datos combinados

121949804 Adam3 a disintegrin and metalloprotease domain 3 (cyritestin) precursor - 0 0 0 - 0 0 0 1 4,7 3 3 1 7,1 4 4 0,0000531 124248577 psmc4 26S protease regulatory subunit 6B - 2,2 1 1 - 2,2 1 1 1 7,7 3 3 1 9,8 4 5 0,000113 124430564 TCTE1 T-complex-associated testis-expressed protein 1 - 4,2 1 1 - 0 0 0 1 9,8 3 3 1 9,8 3 4 0,0000871 124486704 4933401F05Rik serine protease 55 precursor - 3,4 1 2 - 0 0 0 1 13,1 3 3 1 13,1 3 5 0,000331 124486893 1700009P17Rik UPF0740 protein C1orf192 homolog - 4,8 1 1 - 4,8 1 1 1 27,5 4 4 1 27,5 4 10 0,000793 124486895 PGD 6-phosphogluconate dehydrogenase, decarboxylating - 0 0 0 - 2,3 1 1 1 9,3 4 4 1 9,7 4 4 0,0000739 125988403 SPCS3 signal peptidase complex subunit 3 - 5 1 1 - 0 0 0 1 17,8 3 3 1 17,8 3 5 0,000261 12963591 Stoml2 stomatin-like protein 2, mitochondrial - 0 0 0 - 0 0 0 1 19 5 5 1 14,2 4 4 0,0000908 12963697 Fahd1 acylpyruvase FAHD1, mitochondrial - 7 1 1 - 0 0 0 1 22,5 4 4 1 22,5 4 5 0,000198 12963757 isyna1 inositol-3-phosphate synthase 1 - 1,6 1 1 - 0 0 0 1 8,4 5 5 1 6,8 4 6 0,000137 13277394 GRPEL1 grpE protein homolog 1, mitochondrial precursor - 4,1 1 2 - 4,1 1 1 1 13,8 3 3 1 13,8 3 7 0,00025 13384742 Gm10078 28S ribosomal protein S36, mitochondrial isoform 1 - 0 0 0 - 0 0 0 1 44,1 2 2 1 44,1 2 3 0,000427 13385680 DECR1 2,4-dienoyl-CoA reductase, mitochondrial precursor - 0 0 0 - 0 0 0 1 12,2 3 3 0,9989 9,3 2 2 0,0000448 13386034 rps13 40S ribosomal protein S13 - 7,9 1 2 - 6 1 1 1 19,2 3 3 1 20,5 3 6 0,000256 13447394 CLCA2 chloride channel calcium activated 2 precursor - 0 0 0 - 0 0 0 1 11,9 7 7 1 11,9 7 10 0,000136 14149635 FABP4 fatty acid-binding protein, adipocyte - 0 0 0 - 0 0 0 1 37,9 5 5 1 37,9 5 6 0,000244 14192922 Actc1 actin, alpha cardiac muscle 1 - 0 0 0 - 0 0 0 1 39,8 14 14 1 39,8 14 5 0,0000798 149257598 Gm7246 PREDICTED: 40S ribosomal protein S8 isoform 1 - 0 0 0 - 0 0 0 1 21,4 3 3 0,977 7,7 1 1 - 149261044 Gm5428 PREDICTED: 60S ribosomal protein L6-like - 0 0 0 - 0 0 0 1 11,5 4 4 0,9989 5,7 2 2 0,0000412 15277319 Gm6776 leucine zipper transcription factor-like protein 1 - 3,7 1 1 - 3,7 1 1 1 13 3 3 1 22,7 5 5 0,000143 157041252 PLIN4 perilipin-4 - 0 0 0 - 0 0 0 1 21,9 5 5 1 21,9 5 10 0,0000461 157951741 cat catalase - 0 0 0 - 0 0 0 1 16,5 7 7 1 10,6 5 5 0,0000762 158262037 2310061I04Rik uncharacterized protein LOC69662 - 0 0 0 - 0 0 0 1 12,1 4 4 1 9,5 3 5 0,000195 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, 158631246 NDUFB11 mitochondrial - 6,6 1 3 - 6,6 1 3 1 30,5 3 3 0,9992 19,2 2 8 0,000446 160298168 Gbas protein NipSnap homolog 2 - 0 0 0 - 0 0 0 1 13,9 4 4 0,9999 11 3 5 0,0002 160298207 GNPAT dihydroxyacetone phosphate acyltransferase - 0 0 0 - 0 0 0 1 4,4 3 3 1 5,9 4 4 0,0000485 160298209 GOT1 aspartate aminotransferase, cytoplasmic - 0 0 0 - 3,4 1 1 1 9 3 3 1 9 3 4 0,0000663 160298213 LOC630729 glutathione reductase, mitochondrial precursor - 0 0 0 - 0 0 0 1 13,8 4 4 1 13,8 4 4 0,0000814 160333304 APOA1 apolipoprotein A-I preproprotein - 0 0 0 - 4,9 1 1 1 23,1 6 6 1 23,1 6 13 0,000357 160358825 Apoh beta-2-glycoprotein 1 precursor - 0 0 0 - 2,9 1 1 1 22,9 7 7 1 22,9 7 10 0,000221 160707894 Akr1b3 aldose reductase - 0 0 0 - 0 0 0 1 18,7 5 5 1 18,7 5 5 0,000147 160707911 ank1 ankyrin-1 isoform 2 - 0 0 0 - 0 0 0 1 9 12 12 1 7,8 10 16 0,0000792 167234411 NUBPL iron-sulfur protein NUBPL precursor - 0 0 0 - 2,8 1 1 1 17,6 4 4 1 17,6 4 5 0,000145 167716837 ND1 NADH dehydrogenase subunit 1 [Mus musculus musculus] - 0 0 0 - 0 0 0 1 8,8 3 3 0,9994 6,3 2 2 0,000211 170650630 Sirt2 NAD-dependent protein deacetylase sirtuin-2 isoform 1 - 3,4 1 1 - 0 0 0 1 10,5 3 3 1 10,5 3 4 0,0000923 176865892 EIF4A2 eukaryotic initiation factor 4A-II isoform a - 0 0 0 - 0 0 0 1 11 3 3 0 0 3 0 - 188035926 NDUFAF2 mimitin, mitochondrial - 8,3 1 1 - 0 0 0 1 19,6 3 3 1 19,6 3 6 0,000192 19526481 Spna1 spectrin alpha chain, erythrocytic 1 - 0 0 0 - 0,4 1 1 1 11,4 22 22 1 12,2 22 38 0,000122 19526848 MOSC2 MOSC domain-containing protein 2, mitochondrial precursor - 0 0 0 - 2,1 1 1 1 14,2 5 5 1 9,2 3 4 0,0000945 19526858 4930579J09Rik protein phosphatase 1 regulatory subunit 32 - 0 0 0 - 0 0 0 1 45,9 13 13 1 43,3 12 16 0,000292 19526912 st13 hsc70-interacting protein - 2,7 1 1 - 0 0 0 1 14,3 5 5 1 14,3 5 6 0,000156 19527310 PPIF peptidyl-prolyl cis-trans isomerase F, mitochondrial precursor - 0 0 0 - 0 0 0 1 28,2 5 5 1 24,8 4 4 0,000125 205361116 Mdh1b putative malate dehydrogenase 1B - 0 0 0 - 0 0 0 1 11,2 5 5 1 6,6 3 3 0,0000652 20846986 RPS25 PREDICTED: 40S ribosomal protein S25-like - 7,2 1 1 - 0 0 0 1 20,8 3 3 1 20,8 3 5 0,000201

21312062 tmed10 transmembrane emp24 domain-containing protein 10 precursor - 0 0 0 - 0 0 0 1 18,3 3 3 0,9999 18,3 3 3 0,000143

21312204 HDHD3 haloacid dehalogenase-like hydrolase domain-containing protein 3 - 0 0 0 - 0 0 0 1 12,4 3 3 0,9985 8,4 2 2 0,0000991 21313054 IQCD IQ domain-containing protein D - 2 1 1 - 2 1 2 1 12,2 5 5 1 7,6 3 5 0,0000724

21313128 1700034H14Rik mitochondrial import inner membrane translocase subunit Tim21 - 4,1 1 1 - 4,1 1 2 1 11,5 3 3 0,9795 4,1 1 4 - 21313314 EFCAB1 EF-hand calcium-binding domain-containing protein 1 - 0 0 0 - 0 0 0 1 15,1 3 3 0,999 11,8 2 4 0,000148

21450277 ATP1A1 sodium/potassium-transporting ATPase subunit alpha-1 precursor - 0 0 0 - 0 0 0 1 11,9 11 11 1 11,1 10 4 0,0000363 21644577 abhd11 alpha/beta hydrolase domain-containing protein 11 isoform 1 - 5,7 1 2 - 0 0 0 1 32,2 5 5 1 28,6 5 8 0,000342 22094075 LOC631229 ADP/ATP translocase 2 - 11,1 3 1 - 5,7 2 1 1 32,6 9 9 1 32,6 9 11 0,000241 22164770 Oit1 protein FAM3D precursor - 0 0 0 - 0 0 0 1 13 3 3 1 13 3 3 0,000102 delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial 225543103 ALDH4A1 precursor - 0 0 0 - 1,6 1 1 1 13,3 6 6 1 11,9 5 6 0,0000978 iron-sulfur cluster co-chaperone protein HscB, mitochondrial 225703114 hscB precursor - 0 0 0 - 0 0 0 1 27,4 5 5 1 25,6 5 8 0,000221 225703118 Ado 2-aminoethanethiol dioxygenase - 6,6 1 1 - 3,5 1 2 1 20,7 4 4 1 20,7 4 7 0,000223 225735569 ALDH1A2 retinal dehydrogenase 2 - 1,9 1 1 - 0 0 0 1 8,3 3 3 0,9994 4,6 2 3 0,0000511 226453478 COX2 cytochrome c oxidase subunit II [Mus musculus castaneus] - 0 0 0 - 0 0 0 1 40,5 5 5 1 44,9 6 98 0,00974733 227116292 DPP4 dipeptidyl peptidase 4 isoform 2 - 1,4 1 1 - 1,4 1 1 1 8,4 6 6 1 7,4 5 7 0,0000882 227116358 MECR trans-2-enoyl-CoA reductase, mitochondrial precursor - 0 0 0 - 0 0 0 1 20,6 6 6 1 16,4 5 7 0,000172 hydroxyacylglutathione hydrolase, mitochondrial isoform 1 227499238 HAGH precursor - 0 0 0 - 4,6 1 1 1 13,8 3 3 1 13,8 3 7 0,000206 Réplica 1 Réplica 2 Réplica 3 Datos combinados

309262734 Samd15 PREDICTED: sterile alpha motif domain-containing protein 15 - 1,9 1 4 - 2,4 1 1 1 5,6 3 3 0,9998 4,4 2 9 0,000124 309267418 Dnah7 PREDICTED: dynein heavy chain 7, axonemal - 0 0 0 - 0 0 0 1 9,7 33 33 1 9,4 33 14 0,0000302 31543952 WARS2 tryptophan--tRNA ligase, mitochondrial precursor - 0 0 0 - 0 0 0 1 12,8 5 5 1 12,8 5 5 0,000114 31559918 NRD1 nardilysin precursor - 0 0 0 - 0 0 0 1 4,1 4 4 1 5,1 5 5 0,0000466 31560255 Fam82b regulator of microtubule dynamics protein 1 - 5,2 1 1 - 0 0 0 1 17,7 4 4 1 17,7 4 6 0,000169 31560677 LTF lactotransferrin precursor - 0 0 0 - 1,4 1 1 1 5,4 4 4 1 5,4 4 5 0,0000475

31980772 LOC627816 serine/threonine-protein phosphatase PP1-gamma catalytic subunit - 23,2 6 2 - 0 0 0 1 34,4 9 9 1 30,3 8 31 0,000806 31981690 LOC641192 heat shock cognate 71 kDa protein - 0 0 0 - 0 0 0 1 20,9 11 11 1 20,9 11 54 0,00067 31981830 COX7A2 cytochrome c oxidase subunit 7A2, mitochondrial precursor - 12 1 2 - 12 1 2 1 27,7 2 2 1 27,7 2 7 0,000714 31982273 hsd17b4 peroxisomal multifunctional enzyme type 2 - 0 0 0 - 0 0 0 1 6,5 4 4 1 8,2 5 5 0,0000592 long-chain specific acyl-CoA dehydrogenase, mitochondrial 31982520 acadl precursor - 2,8 1 1 - 0 0 0 1 13,7 5 5 1 11,4 4 5 0,0000966 323276641 Crisp2 cysteine-rich secretory protein 2 precursor - 0 0 0 - 0 0 0 1 16 3 3 1 16 3 3 0,00014 334688818 Wdr78 WD repeat-containing protein 78 - 1,4 1 1 - 1,1 1 1 1 8,3 6 6 1 8,3 6 9 0,0000989 34328278 Lman2 vesicular integral-membrane protein VIP36 precursor - 0 0 0 - 0 0 0 1 16,2 3 3 1 16,2 3 4 0,0000902 34328334 Acox3 peroxisomal acyl-coenzyme A oxidase 3 - 0 0 0 - 1,7 1 1 1 13,7 7 7 1 13,7 7 8 0,0000963 34610211 Sort1 sortilin isoform 2 precursor - 0 0 0 - 0 0 0 1 3,8 3 3 0,9987 2,7 2 2 0,0000265 356582489 Ogdh 2-oxoglutarate dehydrogenase, mitochondrial isoform 3 - 0 0 0 - 0 0 0 1 47,8 40 40 #N/A #N/A #N/A #N/A - 363543275 Adgb androglobin - 0 0 0 - 0 0 0 1 3 4 4 1 2,1 3 3 0,0000179 38348576 Odf3l1 outer dense fiber protein 3-like protein 1 - 4,4 1 1 - 4,4 1 1 1 16,4 4 4 1 16,4 4 9 0,000289 38604071 xpo1 exportin-1 - 0 0 0 - 0 0 0 1 3,3 3 3 0,9994 2,1 2 2 0,0000211 39841061 4922502D21Rik C-type lectin-like receptor 2m - 4,2 1 3 - 4,2 1 1 1 14,3 3 3 1 14,3 3 8 0,000347 39930543 NUP54 nuclear pore complex protein Nup54 - 3,3 1 1 - 0 0 0 1 11,4 4 4 1 11,4 4 6 0,000108 40254263 Ubtd2 ubiquitin domain-containing protein 2 - 0 0 0 - 5,6 1 1 1 16,2 4 4 1 20,9 4 7 0,000292 41056093 Nif3l1 NIF3-like protein 1 - 3,5 1 1 - 3,5 1 1 1 21 5 5 1 18,1 4 6 0,000163 42415475 p4hb protein disulfide-isomerase precursor - 0 0 0 - 0 0 0 1 21,6 9 9 1 21,6 9 10 0,000136 45476581 SCP2 non-specific lipid-transfer protein - 0 0 0 - 0 0 0 1 5,3 3 3 0,9989 3,3 2 2 0,0000355 46049022 PSMD6 26S proteasome non-ATPase regulatory subunit 6 - 0 0 0 - 3,3 1 1 1 18 6 6 1 20,6 7 9 0,000157 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 46195798 DDOST kDa subunit precursor - 2,5 1 2 - 2,5 1 1 1 11,6 3 3 1 14,1 4 6 0,000161 46430514 4930504O13Rik uncharacterized protein LOC403200 precursor - 0 0 0 - 7,6 1 2 1 23,4 4 4 1 23,4 4 10 0,000598 55742711 Ehd2 EH domain-containing protein 2 - 0 0 0 - 0 0 0 1 6,6 4 4 1 6,6 4 5 0,0000722 55925585 Spag8 sperm-associated antigen 8 - 0 0 0 - 0 0 0 1 11,7 3 3 1 11,7 3 3 0,0000871 58037095 Spryd4 SPRY domain-containing protein 4 - 4,8 1 1 - 4,8 1 1 1 14,5 3 3 1 14,5 3 5 0,000126 58037325 Dusp21 dual specificity phosphatase 21 - 0 0 0 - 0 0 0 1 13,2 2 2 1 13,2 2 8 0,000871 66392160 VPS13A vacuolar protein sorting-associated protein 13A - 0 0 0 - 0 0 0 1 7,4 18 18 1 7,2 17 17 0,0000629 66773165 acp6 lysophosphatidic acid phosphatase type 6 precursor - 0 0 0 - 0 0 0 1 10 4 4 1 10 4 5 0,000107 6678950 Nudt1 7,8-dihydro-8-oxoguanine triphosphatase precursor - 0 0 0 - 0 0 0 1 25,6 3 3 1 25,6 3 3 0,000196 6679537 Ptgs1 prostaglandin G/H synthase 1 precursor - 2,3 1 1 - 2,3 1 1 1 10,8 6 6 1 9,5 5 7 0,000146 6679567 PTRF polymerase I and transcript release factor - 0 0 0 - 3,8 1 1 1 13 3 3 1 13 3 12 0,000275

6680690 prdx3 thioredoxin-dependent peroxide reductase, mitochondrial precursor - 0 0 0 - 0 0 0 1 17,5 4 4 1 17,5 4 4 0,000149 6680724 Arf6 ADP-ribosylation factor 6 - 6,3 1 1 - 0 0 0 1 24,6 3 3 1 24,6 3 5 0,000249 6680836 CALR calreticulin precursor - 0 0 0 - 0 0 0 1 10,8 4 4 1 8,7 3 3 0,000072 6680854 cav1 caveolin-1 isoform 1 - 0 0 0 - 0 0 0 1 24,2 3 3 0,9984 12,4 2 3 0,000159 6753036 ALDH2 aldehyde dehydrogenase, mitochondrial precursor - 0 0 0 - 1,7 1 1 1 19,3 7 7 1 16,6 6 7 0,000114 6753374 CDH1 cadherin-1 precursor - 0,9 1 1 - 0 0 0 1 3,1 3 3 0,9997 1,9 2 4 0,0000601 6754660 Cd46 membrane cofactor protein precursor - 2,2 1 2 - 0 0 0 1 16,7 5 5 1 16,7 5 14 0,000498 6754724 psmd7 26S proteasome non-ATPase regulatory subunit 7 - 5,3 1 1 - 0 0 0 1 23,7 5 5 1 23,7 5 6 0,000164 6754976 Gm7204 peroxiredoxin-1 - 0 0 0 - 0 0 0 1 38,2 7 7 1 43,2 8 17 0,000567 6755210 PSMD13 26S proteasome non-ATPase regulatory subunit 13 - 2,7 1 1 - 2,7 1 1 1 22,6 8 8 1 18,1 6 8 0,000161 Réplica 1 Réplica 2 Réplica 3 Datos combinados

9910194 dhodh dihydroorotate dehydrogenase (quinone), mitochondrial precursor - 0 0 0 - 0 0 0 1 7,3 3 3 1 7,3 3 4 0,0000898

111038118 hadh hydroxyacyl-coenzyme A dehydrogenase, mitochondrial precursor - 3,5 1 1 - 0 0 0 0,9999 6,7 2 2 0,9998 6,7 2 3 0,0000947 13385904 1700021F05Rik uncharacterized protein C6orf203 homolog - 0 0 0 - 6,2 1 1 0,9999 11,7 2 2 0,9998 11,7 2 3 0,0000878 NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial 140969946 NDUFV3 isoform 2 - 10,6 1 1 - 0 0 0 0,9999 30,8 2 2 0,9998 30,8 2 3 0,000191 149260287 LOC100045999 PREDICTED: GTP-binding nuclear protein Ran-like - 0 0 0 - 8,8 2 2 0,9999 31 6 6 0,9999 36,1 7 7 0,000248 19526900 TMEM30A cell cycle control protein 50A - 2,3 1 1 - 2,3 1 1 0,9999 9,1 2 2 1 11,3 3 5 0,000156 227116343 CD36 platelet glycoprotein 4 - 0 0 0 - 2,5 1 1 0,9999 4,2 2 2 0,9997 4,2 2 3 0,0000828 30425280 4930412F15Rik protein FAM221B - 0 0 0 - 0 0 0 0,9999 6,2 2 2 1 9,9 3 3 0,0000628 31044463 CWH43 PGAP2-interacting protein - 0 0 0 - 0 0 0 0,9999 4,7 2 2 0,9998 4,7 2 2 0,0000324 31542819 FMO2 dimethylaniline monooxygenase [N-oxide-forming] 2 - 0 0 0 - 1,9 1 1 0,9999 5,4 2 2 0,9998 5,4 2 3 0,0000529 31560433 snx3 sorting nexin-3 - 8,6 1 1 - 0 0 0 0,9999 13,6 2 2 1 18,5 3 5 0,000207 58037205 BAG5 BAG family molecular chaperone regulator 5 - 0 0 0 - 0 0 0 0,9999 6 2 2 0,9998 6 2 2 0,0000362 58743353 RNASE13 probable inactive ribonuclease-like protein 13 precursor - 0 0 0 - 0 0 0 0,9999 22,2 3 3 0,9688 8,5 1 1 - 83699424 RPL18 60S ribosomal protein L18 - 0 0 0 - 0 0 0 0,9999 13,8 2 2 0,9997 13,8 2 2 0,0000794 9790219 DSTN destrin - 0 0 0 - 0 0 0 0,9999 13,9 2 2 0,9998 13,9 2 4 0,000127 117606366 CMPK2 UMP-CMP kinase 2, mitochondrial precursor - 0 0 0 - 0 0 0 0,9998 4,7 2 2 0,9996 4,7 2 2 0,0000385 12963555 NIT2 omega-amidase NIT2 - 0 0 0 - 5,4 1 1 0,9998 9,8 2 2 0,9996 9,8 2 3 0,0000766 13385294 PCYOX1 prenylcysteine oxidase precursor - 0 0 0 - 0 0 0 0,9998 7,7 2 2 0,9996 7,7 2 2 0,0000462 146260278 GPX6 glutathione peroxidase 6 precursor - 5,4 1 2 - 5,4 1 2 0,9998 5,4 1 1 0,9997 5,4 1 8 0,000408 22507343 LY6G5B lymphocyte antigen 6 complex locus protein G5b precursor - 0 0 0 - 5,7 1 1 0,9998 16 2 2 0,9996 16 2 3 0,000187 255760052 COPS7A COP9 signalosome complex subunit 7a isoform 2 - 4,4 1 1 - 4,4 1 1 0,9998 8,4 2 2 1 12,7 3 4 0,000121 257196215 Ccdc116 coiled-coil domain-containing protein 116 - 1,5 1 1 - 0 0 0 0,9998 5,2 2 2 0,9995 5,2 2 8 0,000188 28893247 cyb5r2 NADH-cytochrome b5 reductase 2 isoform 2 - 2,9 1 1 - 0 0 0 0,9998 7,2 2 2 0,9996 7,2 2 3 0,000106 31981106 mogs mannosyl-oligosaccharide glucosidase - 0 0 0 - 0 0 0 0,9998 3,6 2 2 0,9996 3,6 2 2 0,0000302 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 34996495 Rpn2 subunit 2 precursor - 0 0 0 - 1,4 1 1 0,9998 4,4 2 2 1 5,9 3 3 0,0000591 377833208 Pdia6 PREDICTED: protein disulfide-isomerase A6 - 3,6 1 1 - 2,8 1 1 0,9998 22 7 7 0,9997 22 7 11 0,000275 59933292 BC049730 uncharacterized protein LOC232972 precursor - 0 0 0 - 0 0 0 0,9998 6,9 2 2 0,9996 6,9 2 3 0,000157 6678099 SERPINE2 glia-derived nexin precursor - 0 0 0 - 0 0 0 0,9998 5,5 2 2 0,9996 5,5 2 2 0,0000493 6679587 Rab1 ras-related protein Rab-1A - 0 0 0 - 0 0 0 0,9998 14,1 3 3 0,9996 14,1 3 4 0,000123 6753670 DPM1 dolichol-phosphate mannosyltransferase - 0 0 0 - 5,4 1 1 0,9998 9,6 2 2 0,9997 9,6 2 3 0,0000792 10946622 fhl5 four and a half LIM domains protein 5 - 0 0 0 - 0 0 0 0,9997 7,4 2 2 0,9994 7,4 2 2 0,0000502 114520613 1110017D15Rik spermatid-specific manchette-related protein 1 isoform 1 - 48,1 12 2 - 0 0 0 0,9997 66,9 16 16 1 66,9 16 83 0,00173035 117676359 Prss52 serine protease 52 precursor - 4,7 1 1 - 4,7 1 1 0,9997 8,7 2 2 0,9996 8,7 2 5 0,000159 13385290 carhsp1 calcium-regulated heat stable protein 1 - 0 0 0 - 0 0 0 0,9997 18,2 2 2 0,9994 18,2 2 5 0,000299 158631221 1700093K21Rik uncharacterized protein C2orf74 homolog - 7,7 1 1 - 0 0 0 0,9997 12,2 2 2 0,9995 12,2 2 3 0,000165

158635979 ATP2A2 sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform a - 0 0 0 - 0 0 0 0,9997 2,6 2 2 0,9994 2,6 2 3 0,0000263 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 164518942 DAD1 subunit DAD1 - 0 0 0 - 0 0 0 0,9997 19,5 2 2 0,9995 19,5 2 2 0,00021 19482172 rdh11 retinol dehydrogenase 11 precursor - 0 0 0 - 4,4 1 1 0,9997 6,6 2 2 1 11,1 3 3 0,0000939 19882207 LOC674674 farnesyl pyrophosphate synthase isoform 2 - 2,3 1 1 - 0 0 0 0,9997 4,8 2 2 0,9995 4,8 2 3 0,000085 21311849 Ggct gamma-glutamylcyclotransferase - 5,9 1 1 - 0 0 0 0,9997 12,8 2 2 0,9995 12,8 2 3 0,000109 21624609 BPHL valacyclovir hydrolase precursor - 0 0 0 - 0 0 0 0,9997 7,2 2 2 0,9994 7,2 2 2 0,0000478 21703344 sar1a GTP-binding protein SAR1a - 0 0 0 - 0 0 0 0,9997 11,6 2 2 0,9995 11,6 2 2 0,0000886 carcinoembryonic antigen-related cell adhesion molecule 10 229092064 Ceacam10 precursor - 4,5 1 1 - 4,5 1 1 0,9997 4,5 1 1 0,9994 4,5 1 4 0,000243 238624186 LOC100047608 glypican-4 precursor - 0 0 0 - 1,8 1 2 0,9997 4,7 2 2 0,9996 4,7 2 5 0,000104 241982740 Mtx1 metaxin-1 isoform 1 - 0 0 0 - 3 1 1 0,9997 6,5 2 2 1 8,5 3 8 0,000147 244791124 DPP3 dipeptidyl peptidase 3 - 0 0 0 - 0 0 0 0,9997 5,8 2 2 0,9995 5,8 2 4 0,0000468

254039596 iscA2 iron-sulfur cluster assembly 2 homolog, mitochondrial precursor - 7,8 1 1 - 0 0 0 0,9997 15,6 2 2 0,9995 15,6 2 3 0,000105 254587975 SATL1 spermidine/spermine N(1)-acetyltransferase-like protein 1 - 1,3 1 1 - 0 0 0 0,9997 3,6 2 2 1 5 3 9 0,000173

27369748 ALDH5A1 succinate-semialdehyde dehydrogenase, mitochondrial precursor - 0 0 0 - 0 0 0 0,9997 4,2 2 2 0,9995 4,2 2 2 0,0000299 293651573 - predicted gene 6760 - 10,3 1 1 - 0 0 0 0,9997 25,3 2 2 0,9995 25,3 2 4 0,000259 306966148 Smpd5 sphingomyelin phosphodiesterase 5 - 2,3 1 2 - 0 0 0 0,9997 4,3 2 2 0,9996 4,3 2 5 0,000138 309265792 Rpl22l1 PREDICTED: 60S ribosomal protein L22-like - 14,6 1 1 - 0 0 0 0,9997 27 2 2 0,9995 27 2 3 0,000201 47059480 SRPK2 SRSF protein kinase 2 - 1,8 1 2 - 1,8 1 1 0,9997 2,9 2 2 0,9994 2,9 2 6 0,000106 Réplica 1 Réplica 2 Réplica 3 Datos combinados

124486652 COQ10A coenzyme Q-binding protein COQ10 homolog A, mitochondrial - 0 0 0 - 0 0 0 0,9996 6,6 2 2 0,9992 6,6 2 2 0,0000575 13385592 LYZL1 lysozyme-like protein 1 precursor - 8,1 1 1 - 8,1 1 2 0,9996 8,1 1 1 0,9995 8,1 1 6 0,000452 148225760 Gm595 uncharacterized protein LOC209005 - 0 0 0 - 0 0 0 0,9996 3,4 2 2 0,9847 2,1 1 1 - 188497650 Mlec malectin precursor - 0 0 0 - 4,5 1 1 0,9996 10,7 2 2 0,9994 10,7 2 3 0,0000766 19527378 TMEM109 transmembrane protein 109 precursor - 0 0 0 - 0 0 0 0,9996 8,6 2 2 0,9993 8,6 2 4 0,000222 21539639 0610037L13Rik UPF0587 protein C1orf123 homolog - 6,1 1 1 - 0 0 0 0,9996 11,2 2 2 0,9992 11,2 2 3 0,00011 21704066 LOC100046373 ras-related protein Rap-1A precursor - 0 0 0 - 5,4 1 1 0,9996 11,4 2 2 0,9994 11,4 2 4 0,000159 23956058 PLP1 myelin proteolipid protein - 4 1 1 - 0 0 0 0,9996 6,9 2 2 0,9992 6,9 2 3 0,000137 28916687 rab5b ras-related protein Rab-5B isoform 1 - 15,8 3 1 - 0 0 0 0,9996 22,3 4 4 0,9993 27 5 3 0,000113 31543773 SSSCA1 Sjoegren syndrome/scleroderma autoantigen 1 homolog - 0 0 0 - 0 0 0 0,9996 17,6 2 2 1 17,6 2 3 0,000163 31981304 atp6v0d1 V-type proton ATPase subunit d 1 - 0 0 0 - 0 0 0 0,9996 6 2 2 0,9993 6 2 2 0,0000506 406855427 Xpnpep1 xaa-Pro aminopeptidase 1 - 0 0 0 - 0 0 0 0,9996 3,3 2 2 0,9993 3,3 2 2 0,0000239 PREDICTED: cytochrome c oxidase subunit 7C, mitochondrial- 407261032 Cox7c like - 14,3 1 1 - 0 0 0 0,9996 28,6 2 2 0,9992 28,6 2 4 0,000492 6677805 Gm8729 40S ribosomal protein S4, X isoform - 3,4 1 1 - 0 0 0 0,9996 6,1 2 2 0,9993 6,1 2 3 0,0000727 111955131 Gm4787 a disintegrin and metalloprotease domain 4b precursor - 0 0 0 - 2,5 2 3 0,9995 7,1 5 5 1 6,7 5 3 0,0000379 13195674 Gm13082 ras-related protein Rab-6A isoform 2 - 0 0 0 - 0 0 0 0,9995 9,6 2 2 0,9991 9,6 2 3 0,0000905 15011843 SEPHS2 selenide, water dikinase 2 - 0 0 0 - 0 0 0 0,9995 4,9 2 2 0,9992 4,9 2 2 0,0000822 157952206 bag1 BAG family molecular chaperone regulator 1 isoform 1L - 0 0 0 - 0 0 0 0,9995 13,2 2 2 1 17,8 3 3 0,0000814 160420337 ENO4 enolase-like protein ENO4 - 1,8 1 1 - 1,8 1 1 0,9995 3,9 2 2 0,9837 1,8 1 3 - 165377271 Dnajb4 dnaJ homolog subfamily B member 4 - 0 0 0 - 0 0 0 0,9995 5,9 2 2 0,9991 5,9 2 2 0,0000433

21312189 iscA1 iron-sulfur cluster assembly 1 homolog, mitochondrial precursor - 10,1 1 1 - 0 0 0 0,9995 19,4 2 2 1 29,5 3 4 0,000189 21312286 1700013G24Rik uncharacterized protein LOC69380 - 0 0 0 - 0 0 0 0,9995 9,8 2 2 0,9992 9,8 2 2 0,0000836 21313138 gstk1 glutathione S-transferase kappa 1 - 0 0 0 - 0 0 0 0,9995 9,3 2 2 0,9841 4,4 1 1 - 226958354 PPM1B protein phosphatase 1B isoform 1 - 2,3 1 1 - 0 0 0 0,9995 10 3 3 0,9955 10,3 4 8 0,000144 succinate dehydrogenase cytochrome b560 subunit, mitochondrial 228008307 sdhC precursor - 4,7 1 2 - 0 0 0 0,9995 12,4 2 2 0,9991 12,4 2 9 0,000644 29126205 ACAA2 3-ketoacyl-CoA thiolase, mitochondrial - 0 0 0 - 0 0 0 0,9995 8,3 2 2 1 10,3 3 3 0,000065 29244306 LOC100044344 transmembrane protein ENSP00000340100 homolog - 5,9 1 1 - 3 1 1 0,9995 10 2 2 1 13 3 4 0,00011

31543108 lamp2 lysosome-associated membrane glycoprotein 2 isoform 2 precursor - 0 0 0 - 0 0 0 0,9995 4,3 2 2 0,999 4,3 2 3 0,0000799 6755040 pfn1 profilin-1 - 0 0 0 - 0 0 0 0,9995 20 2 2 0,999 20 2 2 0,0000866 86198335 NUDT2 bis(5'-nucleosyl)-tetraphosphatase [asymmetrical] - 0 0 0 - 0 0 0 0,9995 15,6 2 2 0,9991 15,6 2 2 0,0000921 9790019 ASAH1 acid ceramidase precursor - 0 0 0 - 2,5 1 1 0,9995 4,8 2 2 0,9997 4,8 2 3 0,0000635 18017605 UBE2N ubiquitin-conjugating enzyme E2 N - 0 0 0 - 0 0 0 0,9994 13,8 2 2 0,9815 7,2 1 2 - 21703810 Ubl4 ubiquitin-like protein 4A - 0 0 0 - 0 0 0 0,9994 12,1 2 2 0,9988 12,1 2 2 0,0000893 242247071 EFCAB10 EF-hand calcium-binding domain-containing protein 10 - 6,8 1 1 - 0 0 0 0,9994 12,1 2 2 0,9989 12,1 2 3 0,000232 27229283 Rexo2 oligoribonuclease, mitochondrial precursor - 0 0 0 - 4,2 1 1 0,9994 8,9 2 2 0,9993 7,6 2 3 0,0000998 31543974 YWHAB 14-3-3 protein beta/alpha - 0 0 0 - 0 0 0 0,9994 18,3 5 5 0,9989 17,9 4 2 0,0000681 47059013 KRT73 keratin, type II cytoskeletal 73 - 0 0 0 - 0 0 0 0,9994 4,3 2 2 0 0 3 0 - 52138554 LOC100045772 multidrug resistance-associated protein 9 - 0 0 0 - 1 1 1 0,9994 2,5 2 2 1 3,4 3 3 0,0000247 110224442 TXNRD1 thioredoxin reductase 1, cytoplasmic isoform 2 - 0 0 0 - 0 0 0 0,9993 4,6 2 2 0,9746 2,4 1 1 - serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit 47059051 Gm13169 alpha isoform - 0 0 0 - 0 0 0 0,9993 4,3 2 2 0,981 2,7 1 1 - 7948999 Prdx4 peroxiredoxin-4 precursor - 6,6 2 4 - 0 0 0 0,9993 9,5 3 3 0,9747 6,6 2 3 - delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial 7949037 ech1 precursor - 0 0 0 - 0 0 0 0,9993 5,5 2 2 0,9988 5,5 2 2 0,0000505 114205406 Mcpt3 mast cell protease 4 precursor - 0 0 0 - 0 0 0 0,9992 13 2 2 0,9987 13 2 2 0,0000888 21362335 CPA5 carboxypeptidase A5 precursor - 0 0 0 - 1,8 1 1 0,9992 3,4 2 2 0,9786 1,8 1 2 - 225579132 Txndc2 thioredoxin domain-containing protein 2 isoform 2 - 6,6 1 1 - 0 0 0 0,9992 9,6 2 2 0,9987 9,6 2 5 0,0000829 29243988 LOC100046898 ubiquitin-conjugating enzyme E2 R1 - 4,7 1 1 - 0 0 0 0,9992 8,1 2 2 0,9741 4,7 1 2 - 6755592 SNCG gamma-synuclein - 0 0 0 - 0 0 0 0,9992 23,6 2 2 0,9987 23,6 2 2 0,0000791 86198316 Pdia4 protein disulfide-isomerase A4 precursor - 0 0 0 - 1,7 1 1 0,9992 2,5 2 2 1 4,2 3 3 0,000035 110347525 spata6 spermatogenesis-associated protein 6 precursor - 0 0 0 - 0 0 0 0,9991 4,1 2 2 0,9983 4,1 2 2 0,0000325 118150478 4930578N16Rik coiled-coil domain-containing protein 173 - 0 0 0 - 0 0 0 0,9991 4,8 2 2 0,9723 2,9 1 3 - 256773297 Gm7684 E3 ubiquitin-protein ligase MARCH5 isoform 1 - 0 0 0 - 0 0 0 0,9991 11,9 2 2 0,9985 11,9 2 2 0,0000581 83776583 Defb23 beta-defensin 23 precursor ------0,9991 12,7 2 2 1 22,3 3 4 0,000194

Proteínas identificadas tras tener en cuenta mutaciones puntuales 6679066 Nipsnap1 protein NipSnap homolog 1 1 0,998 8,1 2 0 0 0 0 1 0,995 8,1 2 0,9995 8,1 2 3 8,65E-05 7305027 Eno2 gamma-enolase 0 0 0 0 0 0 0 0 1 0,9942 14,1 13 1 0 3 0 - Tabla suplementaria 8. Datos de MS para las proteínas identificadas en la especie Mus spretus.

Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 10048462 SLC25A20 mitochondrial carnitine/acylcarnitine carrier protein 1 23,9 6 13 1 45,8 11 14 1 21,9 7 15 1 48,5 12 43 0,000951 100816764 CLMN calmin isoform a 1 5,6 5 8 1 4,1 3 3 1 4,1 4 6 1 8,6 8 20 0,000165 acyl-CoA dehydrogenase family member 9, 100817933 ACAD9 mitochondrial 1 21,9 11 18 1 36,3 18 32 1 25,1 13 24 1 36,6 19 72 0,000742 102468565 LOC100044101thioredoxin reductase 2, mitochondrial precursor 1 10,1 3 3 1 10,2 4 5 1 9,7 3 4 1 12,1 5 12 0,000196 calcium-binding tyrosine phosphorylation-regulated 109255220 Cabyr protein isoform a 1 22,5 8 42 1 27,2 10 64 0,9868 24,7 9 4 1 27,2 10 16 0,000426 10946592 Dbil5 diazepam-binding inhibitor-like 5 1 44,8 3 6 1 54 4 15 1 54 4 14 1 54 4 34 0,001811525 10946936 ak1 adenylate kinase isoenzyme 1 isoform 1 1 52,6 9 22 1 68 12 44 1 72,2 14 57 1 72,7 15 121 0,002974642 10946940 RAB2A ras-related protein Rab-2A 1 70,8 15 121 1 75 16 97 1 75 18 121 1 75 18 352 0,008857603 109948267 4930578I06Rik uncharacterized protein C8orf74 homolog 1 15,1 3 3 1 13,4 3 3 1 22,5 5 5 1 22,5 5 10 0,000286 110347483 AKAP4 A-kinase anchor protein 4 isoform b 1 20,7 14 27 1 48,5 35 400 1 47,4 33 387 1 47,6 34 788 0,005202227 tetratricopeptide repeat protein 19, mitochondrial 110347537 TTC19 isoform 1 1 9 3 3 1 11,2 4 4 1 14 4 4 1 11,2 4 10 0,000212 11055972 PRSS21 testisin precursor 1 23,8 6 21 1 20,4 5 19 1 23,8 6 23 1 23,8 6 63 0,003042901 110625624 TCP1 T-complex protein 1 subunit alpha 1 45,7 21 33 1 33,1 15 25 1 39 18 36 1 47,7 22 95 0,001056226 110625692 BC061194 uncharacterized protein LOC381350 1 16,3 7 9 1 16,9 8 4 1 23,8 9 7 1 23,8 9 18 0,000314 110625839 NME5 nucleoside diphosphate kinase homolog 5 1 23,2 4 5 1 35,5 6 8 1 27 5 7 1 35,5 6 18 0,000717 110625871 TEKT4 tektin-4 1 22,8 8 10 1 43 17 36 1 36,7 14 27 1 43 17 72 0,000878 NADH dehydrogenase [ubiquinone] flavoprotein 2, 110625954 Ndufv2 mitochondrial isoform 1 precursor 1 34,3 8 28 1 39,9 9 26 1 41,1 9 34 1 50,8 11 90 0,002342213 110626050 them4 acyl-coenzyme A thioesterase THEM4 1 30,4 7 9 1 21,3 5 11 1 33,9 8 13 1 33,9 8 33 0,001125174 sperm acrosome membrane-associated protein 3 110626054 SPACA3 precursor 1 30,1 4 5 1 36,8 5 11 1 30,1 4 6 1 36,8 5 22 0,001056748 110626086 Arrdc5 arrestin domain-containing protein 5 1 21,5 7 8 1 16 5 5 1 16 5 6 1 24,3 8 20 0,000411

111955131 Gm4787 a disintegrin and metalloprotease domain 4b precursor 1 11,5 9 12 1 13,5 9 12 1 15,1 10 14 1 15,2 11 37 0,0004 complement component 1 Q subcomponent-binding 112181167 c1qbp protein, mitochondrial 1 17,2 3 7 1 26,9 4 6 1 21,5 3 8 1 26,9 4 21 0,000732 cytochrome c oxidase subunit 5A, mitochondrial 112181182 COX5A precursor 1 28,8 6 18 1 53,4 8 21 1 53,4 8 23 1 53,4 8 64 0,003235071 112293264 PDIA3 protein disulfide-isomerase A3 precursor 1 6,9 3 4 1 34,1 13 13 1 32,1 14 16 1 38,8 17 34 0,000402 112734843 Tssk2 testis-specific serine/threonine-protein kinase 2 1 7,8 3 5 0,9901 11,5 3 2 1 16,2 5 5 1 18,4 6 14 0,000239 112734851 Tssk1 testis-specific serine/threonine-protein kinase 1 1 23,3 7 13 1 22,2 7 15 1 24,1 7 17 1 43 13 45 0,00073 112807195 Gm11273 cytochrome c oxidase subunit 5B, mitochondrial 1 46,5 5 11 1 53,5 7 16 1 47,3 6 14 1 47,3 6 40 0,001792688 112983636 KRT10 keratin, type I cytoskeletal 10 1 8,9 5 15 0,9998 6,6 4 11 1 8,9 5 11 1 8,9 5 27 0,000604 113679811 BANF2 barrier-to-autointegration factor-like protein 1 30 3 3 0,9711 14,4 1 1 0 0 0 0 1 44,4 4 5 0,000238 113865881 Gm6685 cytochrome b5 domain-containing protein 1 1 12,3 3 3 0,9893 5,7 1 1 0,9859 5,7 1 1 1 12,3 3 5 0,000178 113866007 Igbp1b immunoglobulin-binding protein 1b 1 16,3 5 22 1 16,3 5 15 1 18,7 6 13 1 18,7 6 50 0,001211404 113866024 rab5c ras-related protein Rab-5C 1 47,7 7 9 1 36,6 6 12 1 60,6 8 13 1 60,6 8 34 0,001053306 113931164 Fndc8 fibronectin type III domain-containing protein 8 1 19,3 4 14 1 21,8 5 19 1 50,5 11 34 1 43,3 10 63 0,001672348 114145546 Dnaic2 dynein intermediate chain 2, axonemal 1 17,5 9 10 1 20,2 11 19 1 22,2 11 17 1 25 13 46 0,000592 114158691 Cops8 COP9 signalosome complex subunit 8 1 20,1 3 3 0,9996 16,7 2 2 1 23,9 3 4 1 30,1 4 9 0,000456 114326406 Acrv1 acrosomal protein SP-10 precursor 1 17,6 3 15 1 18 4 33 1 17,6 3 30 1 18 4 76 0,006702331 complement decay-accelerating factor, GPI-anchored 114326522 Cd55 precursor 1 12,3 4 8 1 15,6 4 9 1 17,7 5 13 1 17,7 5 29 0,000786 spermatid-specific manchette-related protein 1 isoform 114520613 1110017D15Rik1 1 28,5 6 7 1 60,4 14 3 1 58,1 13 32 1 64,6 15 69 0,001230741 disintegrin and metalloproteinase domain-containing 114842412 Adam1b protein 1b precursor 1 5,6 4 5 1 6,3 4 5 1 5,6 4 5 1 7,8 5 15 0,000203 11496255 LOC100048331dnaJ homolog subfamily A member 4 1 8,6 3 5 0,9999 6 2 4 1 22,9 6 8 1 22,9 6 17 0,000273 4-trimethylaminobutyraldehyde dehydrogenase 115334671 ALDH9A1 precursor 1 9,1 4 5 1 20,5 9 11 1 23,7 9 11 1 33,4 13 31 0,000397 116014342 bsg basigin isoform 2 precursor 1 17,6 5 36 1 17,9 6 41 1 17,9 6 33 1 17,9 6 115 0,00358995 116089322 lonp1 lon protease homolog, mitochondrial precursor 1 3,9 4 4 1 11 8 9 1 15,5 10 11 1 17,6 12 24 0,000189 116734870 LTA4H leukotriene A-4 hydrolase 1 9 4 4 0,9997 3,6 2 2 0,9995 4,3 2 2 1 11,3 5 9 0,000105 116805338 3110056O03Riksignal peptide peptidase-like 2B precursor 1 8 3 3 1 7,8 3 3 1 8 3 3 1 10,6 4 9 0,000214 117606409 wbp2nl postacrosomal sheath WW domain-binding protein 1 8,6 3 9 0,9898 3,3 1 6 1 13,6 4 9 1 13,6 4 24 0,000715 117935034 1700061G19Rikuncharacterized protein LOC78625 1 5,7 4 6 1 21,8 11 14 1 15 8 11 1 21,8 11 31 0,000361 117956379 tekt1 tektin-1 1 38,5 14 40 1 36,8 14 32 1 32,5 12 23 1 46,4 17 95 0,001421598

118026915 EFCAB5 EF-hand calcium-binding domain-containing protein 5 1 2,8 4 6 1 4,3 6 8 1 10 9 12 1 11,2 11 26 0,000134 118150641 ropn1l ropporin-1-like protein 1 16,1 4 4 1 15,1 4 4 1 15,6 3 6 1 20,2 5 14 0,000388 119709839 Adam6a a disintegrin and metalloprotease domain 6 1 14,3 9 16 1 8,2 5 3 0,9996 10,1 6 3 1 17,6 11 13 0,000143 120407035 Spam1 hyaluronidase PH-20 precursor 1 16,8 8 23 1 19,7 10 17 1 17,8 8 12 1 26,6 12 54 0,000796 120587011 SYNGR4 synaptogyrin-4 1 15 3 22 1 15 3 17 1 34,3 4 17 1 34,3 4 56 0,004050254 12331398 SCAMP2 secretory carrier-associated membrane protein 2 1 9,7 2 11 1 11,9 3 17 1 9,7 2 8 1 14 4 36 0,002927815

124244035 PPEF1 serine/threonine-protein phosphatase with EF-hands 1 1 8,2 5 6 1 12,2 7 8 1 10,3 6 6 1 18,6 10 20 0,000256 124248577 psmc4 26S protease regulatory subunit 6B 1 9,8 4 6 1 7,9 3 4 1 13,9 6 7 1 16,7 7 18 0,000352 124339838 Hspa1l heat shock 70 kDa protein 1-like 1 39,5 18 20 1 45,6 23 46 1 44,9 22 45 1 49,9 25 107 0,001093426 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 124358957 TTC25 tetratricopeptide repeat protein 25 1 11,5 5 8 1 15,4 7 26 1 16,7 7 27 1 19,1 8 59 0,00064 transmembrane and coiled-coil domain-containing 124486795 Tmco2 protein 2 1 13,9 2 4 1 24,6 4 6 1 23,5 4 8 1 33,7 5 18 0,001048317

124486895 PGD 6-phosphogluconate dehydrogenase, decarboxylating 1 7,7 3 5 1 16,6 7 7 1 9,9 4 4 1 15,9 7 15 0,00024 124487093 ARMC4 armadillo repeat-containing protein 4 1 9 7 16 1 24,9 18 35 1 23,5 17 42 1 27,1 20 93 0,000647 phospholipase B1, membrane-associated isoform 1 124487227 PLB1 precursor 1 7,4 9 9 1 10,8 10 13 1 13,3 14 16 1 16,6 16 40 0,000217 124487327 ARMC3 armadillo repeat-containing protein 3 isoform 1 1 8,4 7 8 1 17,7 15 30 1 12,6 10 17 1 20,5 15 52 0,000388 124487358 1700009N14Rikuncharacterized protein LOC75471 1 13,9 3 3 0,9971 25 5 5 0,9998 25 5 5 0,9999 36,1 7 8 0,000211 124487419 KRT2 keratin, type II cytoskeletal 2 epidermal 1 5,8 4 6 1 5,9 4 10 1 4,7 3 5 1 7,1 5 20 0,00033 1-phosphatidylinositol 4,5-bisphosphate 125347158 PLCD4 phosphodiesterase delta-4 isoform 1 1 13,9 9 11 1 5,3 3 6 1 7,1 4 4 1 15,9 11 24 0,000336 125630303 CYLC1 cylicin-1 1 8 4 7 1 14,5 8 18 1 13,6 8 19 1 16,1 9 43 0,000448 126032329 Eef1a1 elongation factor 1-alpha 1 1 10,3 3 5 0 0 0 0 0 0 0 0 0 0 9 0 - 126157525 GAS8 growth arrest-specific protein 8 1 19,7 9 12 1 9,6 4 8 1 13 6 11 1 23,2 11 30 0,000374 126521835 CCT2 T-complex protein 1 subunit beta 1 26,2 10 18 1 39,1 15 32 1 44,7 19 42 1 44,7 19 92 0,001026517 126723336 PHB2 prohibitin-2 1 51,2 13 39 1 51,2 13 47 1 50,2 13 62 1 58,2 15 147 0,002803321 126723461 CCT8 T-complex protein 1 subunit theta 1 17,9 8 14 1 37,8 17 22 1 42 19 28 1 44 20 62 0,000621 12963569 Ppp1r7 protein phosphatase 1 regulatory subunit 7 1 11,1 4 10 1 19,7 6 6 1 8,6 3 6 1 19,7 6 22 0,000386 NADH dehydrogenase [ubiquinone] 1 alpha 12963571 Ndufa7 subcomplex subunit 7 1 38,9 4 5 1 38,9 6 8 1 39,8 6 9 1 39,8 7 22 0,000897 12963591 Stoml2 stomatin-like protein 2, mitochondrial 1 22,9 6 11 1 22,9 6 6 1 22,9 6 7 1 22,9 6 24 0,000467 NADH dehydrogenase [ubiquinone] 1 alpha 12963633 Ndufa13 subcomplex subunit 13 1 21,5 3 6 1 48,6 7 21 1 48,6 7 14 1 48,6 7 41 0,001577929 131889222 SDR39U1 epimerase family protein SDR39U1 1 21,8 7 15 1 21,8 7 15 1 19,5 6 17 1 21,8 7 47 0,001403069 NADH dehydrogenase [ubiquinone] 1 alpha 13195624 NDUFA10 subcomplex subunit 10, mitochondrial precursor 1 44,2 12 25 1 42 14 29 1 47,9 12 32 1 56,3 15 89 0,001794734 13195690 Gm5928 ubiquitin-40S ribosomal protein S27a precursor 1 44,6 3 26 1 44,6 3 27 1 44,6 3 30 1 44,6 3 85 0,003168195 13249349 RANBP17 ran-binding protein 17 1 11,8 11 17 1 18,2 16 20 1 19,2 17 23 1 25,2 22 59 0,000466 CUB and zona pellucida-like domain-containing 133778951 CUZD1 protein 1 precursor 1 9,9 5 6 1 11 5 8 1 22,6 10 23 1 24,2 11 40 0,000729 13384638 Rsph1 radial spoke head 1 homolog 1 19,9 4 11 1 29,2 5 14 1 32,6 6 12 1 32,6 6 39 0,001243148 13384778 pgls 6-phosphogluconolactonase 1 15,6 3 3 0,9995 9,7 2 2 0,9996 12,1 2 2 1 15,6 3 7 0,0002 13384810 ACYP1 acylphosphatase-1 1 39,4 3 3 1 48,5 4 4 1 36,4 3 3 1 48,5 4 11 0,000542 13385006 cyc1 cytochrome c1, heme protein, mitochondrial 1 40,3 8 29 1 40,3 8 25 1 35,4 7 37 1 40,3 8 92 0,003174415 NADH dehydrogenase [ubiquinone] 1 beta subcomplex 13385054 NDUFB3 subunit 3 1 33,7 3 4 0,9999 26,9 2 3 0,9855 10,6 1 2 1 33,7 3 9 0,000497 13385124 ribC1 RIB43A-like with coiled-coils protein 1 1 10 3 9 1 14,2 4 8 1 14,2 4 11 1 14,2 4 27 0,000422 cytochrome b-c1 complex subunit Rieske, 13385168 uqcrfs1 mitochondrial 1 36,1 9 31 1 43,4 11 43 1 47,4 11 42 1 52,6 12 117 0,003311531 13385180 Spesp1 sperm equatorial segment protein 1 precursor 1 40,9 13 119 1 40,9 13 146 1 38,8 12 139 1 42,9 14 398 0,009194719 13385188 KLHL10 kelch-like protein 10 1 21,4 10 12 1 11 5 5 1 21,1 11 11 1 25,8 13 27 0,000283 13385274 LOC100045163phospholipase B-like 1 precursor 1 12 6 15 1 24,4 10 16 1 26,5 11 17 1 28,5 13 49 0,000788 13385304 WWP2 NEDD4-like E3 ubiquitin-protein ligase WWP2 1 3,7 3 3 0,9846 1,4 1 1 0,9984 2,2 2 2 1 6,1 5 6 0,0000576

13385328 FANK1 fibronectin type 3 and ankyrin repeat domains 1 protein 1 17,4 5 8 1 14,8 4 4 1 11,3 3 3 1 20,3 6 16 0,000272 13385384 PSMD12 26S proteasome non-ATPase regulatory subunit 12 1 6,6 3 4 1 17,5 8 9 1 14,5 8 8 1 22,6 11 22 0,000299 NADH dehydrogenase [ubiquinone] 1 alpha 13385492 ndufa6 subcomplex subunit 6 1 37,4 4 8 1 45,8 5 9 1 39,7 4 6 1 45,8 5 23 0,001093545 NADH dehydrogenase [ubiquinone] 1 beta subcomplex 13385558 Ndufb8 subunit 8, mitochondrial precursor 1 29,6 4 9 1 41,4 5 10 1 46,2 6 18 1 46,2 6 37 0,00126805 13385726 Gm2962 cytochrome b-c1 complex subunit 7 1 27,9 4 8 1 34,2 5 9 1 36,9 6 8 1 28,8 5 21 0,000883 13385730 GLIPR1L2 GLIPR1-like protein 2 1 19,3 5 31 1 15,7 4 15 1 22,3 5 17 1 25,6 6 64 0,002062109 13385762 Lrrc18 leucine-rich repeat-containing protein 18 isoform 1 1 13,7 3 7 0,9883 4,3 1 3 1 16,5 3 7 1 22 4 18 0,000436 13385766 Ubxn11 UBX domain-containing protein 11 1 8,3 3 7 1 10,1 4 5 1 10,1 4 5 1 10,1 4 18 0,000266 13385942 CS citrate synthase, mitochondrial precursor 1 37,5 12 34 1 42,2 14 117 1 42,2 14 125 1 44,2 15 326 0,005511836 13385998 Trap1 heat shock protein 75 kDa, mitochondrial precursor 1 15 9 9 1 20 10 10 1 9,8 6 6 1 24,2 13 25 0,000234 13386040 atp5s ATP synthase subunit s, mitochondrial precursor 1 13,5 3 4 1 21,5 4 8 1 21 4 10 1 21,5 5 24 0,00071 13386062 APOOL apolipoprotein O-like precursor 1 18,5 4 4 1 24,5 5 5 1 23 5 5 1 27,2 6 14 0,000294 NADH dehydrogenase [ubiquinone] 1 alpha 13386100 NDUFA5 subcomplex subunit 5 1 45,7 6 9 1 45,7 6 8 1 45,7 6 9 1 45,7 6 26 0,001397364

13386110 Efcab2 EF-hand calcium-binding domain-containing protein 2 1 22,6 3 3 0,9998 22,6 2 2 1 29,3 3 3 1 46,3 5 8 0,000424 13386316 Actrt2 actin-related protein T2 1 9,3 3 6 1 9,3 3 3 1 11,4 4 5 1 14,3 5 15 0,000284 134031980 Irgc1 interferon-inducible GTPase 5 1 21 8 9 1 36,9 13 31 1 35 12 42 1 36,9 13 79 0,001088223 134031994 psmA2 proteasome subunit alpha type-2 1 26,1 5 5 1 34,6 6 10 1 38,9 7 13 1 47,4 8 28 0,001033864 134053905 Psmd11 26S proteasome non-ATPase regulatory subunit 11 1 20,9 8 13 1 33,2 12 14 1 30,1 12 12 1 38,2 15 39 0,000573 134053952 wdr16 WD repeat-containing protein 16 1 19,8 11 13 1 34,7 18 40 1 30,3 17 44 1 40 20 98 0,001084457 134053957 SLC44A5 choline transporter-like protein 5 1 14,1 8 14 1 18 9 14 1 17 10 18 1 21,4 12 47 0,000672 13507710 Ropn1 ropporin-1 1 59,4 8 53 1 81,6 11 90 1 81,6 11 82 1 81,6 11 227 0,005343302 139948802 Uchl3 ubiquitin carboxyl-terminal hydrolase isozyme L3 1 34,3 6 8 1 43 7 9 1 43 7 13 1 43 7 30 0,000964 145301549 Hba-a1 hemoglobin alpha, adult chain 2 1 35,2 4 10 1 61,3 6 9 1 35,2 4 7 1 61,3 6 25 0,001116662 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 146262012 DDI1 protein DDI1 homolog 1 1 10,8 3 5 0,9996 6,6 2 2 1 9,3 3 3 1 16,7 5 10 0,000259 14780884 POLDIP2 polymerase delta-interacting protein 2 1 10,9 3 6 0,957 3,3 1 1 1 13,9 4 4 1 21,5 6 12 0,000221 148225693 TEKT2 tektin-2 1 32,1 11 31 1 40,9 15 40 1 40,2 15 36 1 44,7 17 106 0,001364028 phosphatidylethanolamine-binding protein 4 isoform a 148230380 Pebp4 precursor 1 24,8 5 13 1 27,7 6 17 1 31,4 7 22 1 31,4 7 53 0,001779406 [Pyruvate dehydrogenase [acetyl-transferring]]- 148277602 Pdp1 phosphatase 1, mitochondrial isoform b 1 9,1 4 4 0,9998 5,8 2 3 1 15,4 5 5 1 19,9 7 12 0,000173 PREDICTED: ATP synthase subunit O, mitochondrial- 149251053 Gm5436 like 1 57,7 9 32 1 61 10 39 1 57,7 9 43 1 61 10 115 0,003181558 PREDICTED: phosphate carrier protein, mitochondrial- 149258501 SLC25A3 like isoform 1 1 11,5 4 7 1 28,6 9 19 1 26,3 8 17 1 28,6 9 43 0,000944 PREDICTED: NADH dehydrogenase [ubiquinone] 1 149266669 Gm3244 beta subcomplex subunit 4-like 1 19,4 3 3 1 46,5 4 5 1 27,1 4 4 1 46,5 5 12 0,000733 PREDICTED: glyceraldehyde-3-phosphate 149273202 Gm4335 dehydrogenase-like isoform 2 1 39,3 9 18 1 50,2 12 31 1 55 14 53 1 55 14 100 0,002040427 PREDICTED: ES1 protein homolog, mitochondrial-like 149274885 LOC100046684isoform 1 1 20,7 4 6 1 39,8 7 12 1 50,4 8 12 1 50,4 8 27 0,000691 152963553 1190002A17Rikadenylate kinase 8 1 34,7 14 38 1 32,8 13 28 1 26,1 11 19 1 41,3 17 85 0,001003532 153791341 Rsph3b radial spoke head protein 3 homolog B 1 12,9 4 19 1 12,1 4 12 1 7,2 3 15 1 14,9 5 44 0,000881 153791370 odf3 outer dense fiber protein 3 1 43,7 9 39 1 69,7 14 53 1 60,6 13 60 1 69,7 14 154 0,004100466 153792273 LOC100046510dynein heavy chain 8, axonemal 1 2,5 11 14 1 19,7 80 145 1 15,4 61 105 1 22 90 249 0,000425 154091013 CCDC135 coiled-coil domain-containing protein lobo homolog 1 7,4 5 6 1 10,7 8 11 1 7,5 5 6 1 13,7 10 24 0,000207 154146209 Usp7 ubiquitin carboxyl-terminal hydrolase 7 1 7,5 7 7 1 9,7 8 10 1 16 15 16 1 20,5 18 32 0,000217 156255157 Acad10 acyl-CoA dehydrogenase family member 10 1 10,5 10 5 1 10,5 10 4 1 13,8 13 7 1 13,8 13 15 0,000102 ATP-binding cassette, sub-family A (ABC1), member 157057150 Abca17 17 1 1,9 3 3 1 3,4 5 5 1 5,6 8 8 1 5,6 8 17 0,000108 157785665 dtymk thymidylate kinase isoform 1 1 16 3 3 1 14,6 3 3 1 22,6 4 4 1 27,8 5 10 0,000267

157909797 tomm40 mitochondrial import receptor subunit TOM40 homolog 1 11,4 3 5 1 21,1 4 7 1 24,1 5 5 1 28 6 17 0,000504 157951596 Car2 carbonic anhydrase 2 1 28,1 4 5 1 42,7 7 10 1 33,1 5 9 1 42,7 7 23 0,000887 157951639 4930404N11Rikuncharacterized protein C19orf71 homolog 1 24,3 4 11 1 24,3 4 11 1 24,3 4 10 1 24,3 4 31 0,001126056 157951670 CAPZA3 F-actin-capping protein subunit alpha-3 1 12,7 4 9 1 25,1 7 10 1 21,7 6 10 1 25,1 7 30 0,000651 157951743 H2-Ke6 estradiol 17-beta-dehydrogenase 8 1 58,7 10 19 1 58,7 10 32 1 62,9 10 33 1 68 11 85 0,002737824 cation channel sperm-associated protein subunit gamma 157951745 Catsperg2 2 precursor 1 7,9 8 14 1 7,2 7 7 0,9998 2,2 2 2 1 9 9 23 0,000197 158187507 Fam71a uncharacterized protein LOC619288 1 15,6 9 23 1 21,2 12 27 1 20 11 30 1 21,4 13 78 0,001124802 158853992 Gm5506 alpha-enolase 1 50,7 16 86 1 54,1 17 127 1 52,5 16 111 1 54,1 17 320 0,004177317 158854020 echdc1 ethylmalonyl-CoA decarboxylase isoform a 1 11 3 3 0,9888 3,7 1 1 0,9856 4 1 1 1 11 3 5 0,000109 158937279 mmel1 membrane metallo-endopeptidase-like 1 1 5,7 4 4 1 11,1 7 9 1 12,5 8 9 1 17,1 11 22 0,00024 160298213 LOC630729 glutathione reductase, mitochondrial precursor 1 9,6 3 3 1 24,8 7 7 1 9,6 3 3 1 24,8 7 13 0,000233 160333212 Canx calnexin precursor 1 11,2 6 6 1 6,4 3 3 1 9,8 4 6 1 18,8 10 17 0,000306 160358791 AKAP3 A-kinase anchor protein 3 1 8 6 20 1 39 28 210 1 37,3 27 170 1 39,8 28 385 0,002906583 succinyl-CoA:3-ketoacid coenzyme A transferase 2B, 160358827 LOC100046138mitochondrial precursor 1 59,8 22 170 1 74,2 28 498 1 70,2 27 444 1 74,4 29 1097 0,009599666 160707894 Akr1b3 aldose reductase 1 10,4 3 3 1 19,6 5 5 1 32,6 9 9 1 38,3 10 17 0,00042 161016820 DNPEP aspartyl aminopeptidase isoform b 1 26,4 10 42 1 34,9 13 40 1 43,1 16 56 1 41,6 15 135 0,002349307 161016824 LOC100045796cysteine desulfurase, mitochondrial 1 19,8 7 15 1 30,5 11 14 1 36,6 13 21 1 42,9 16 52 0,000696 leucine-rich repeat and death domain-containing protein 161086957 4932412H11Rik1 1 7,5 5 7 1 17,4 11 16 1 11,1 7 8 1 18,6 12 29 0,0003 161484642 LOC100044663apolipoprotein O isoform 1 1 26,2 4 11 1 26,2 4 6 1 32,3 5 14 1 32,3 5 32 0,001398155 serine/threonine-protein phosphatase PP1-beta catalytic 161484668 PPP1CB subunit 1 26,9 7 19 1 30,6 8 3 1 38,8 10 4 1 41,3 11 12 0,000262 161760667 lmna prelamin-A/C isoform C 1 17,1 9 13 0 0 0 0 1 8,5 5 6 1 20,7 11 19 0,00018 carnitine O-palmitoyltransferase 2, mitochondrial 162138915 cpt2 precursor 1 30,4 15 22 1 29,9 16 33 1 39,2 18 29 1 42,6 21 79 0,000952 162139827 ME1 NADP-dependent malic enzyme isoform 1 1 22,6 11 15 1 28,3 13 21 1 36,6 14 20 1 39,7 17 56 0,000752 162287165 Cpt1b carnitine O-palmitoyltransferase 1, muscle isoform 1 11,7 8 8 1 27,7 18 27 1 20,3 14 18 1 30,3 21 52 0,000594 162287182 SPAG17 sperm-associated antigen 17 1 4,8 8 9 1 3,3 6 9 1 4 8 11 1 7,8 14 32 0,00013 162329596 PPIL6 peptidyl-prolyl cis-trans isomerase-like 6 1 11,8 3 3 1 19,5 4 5 1 23 4 4 1 29,7 6 13 0,000416 162461907 HSPA9 stress-70 protein, mitochondrial 1 38 19 34 1 45,5 23 93 1 45,8 24 91 1 50,7 26 217 0,001881744 163310765 alb serum albumin precursor 1 5,8 3 14 1 10,2 6 18 1 14 8 21 1 15,5 9 53 0,000534 163644329 APOE apolipoprotein E precursor 1 11,9 3 3 0,9895 2,9 1 1 1 10,3 3 4 1 19,3 5 8 0,000163

163838641 Atp5c1 ATP synthase subunit gamma, mitochondrial isoform a 1 27,4 7 22 1 46,4 10 34 1 43,4 9 36 1 46,4 10 92 0,002450581 163965436 Odf2 outer dense fiber protein 2 isoform b 1 29,6 18 89 0 0 0 0 0 0 0 0 0 0 30 0 - 164519120 CCDC42 coiled-coil domain-containing protein 42A 1 27,5 7 14 1 13 4 5 1 23,7 6 7 1 27,5 7 26 0,00051 164565394 DNAJC11 dnaJ homolog subfamily C member 11 1 13,6 6 7 1 19,1 9 16 1 19,7 9 13 1 22 10 36 0,000431 carcinoembryonic antigen-related cell adhesion 164565419 Ceacam2 molecule 2 isoform a precursor 1 19,1 4 6 1 14,7 3 3 1 19,1 4 4 1 19,1 4 13 0,000282 164607162 Dnalc1 dynein light chain 1, axonemal 1 22,6 4 7 1 22,6 4 6 1 29,5 5 13 1 29,5 5 26 0,000844 165932389 NUP155 nuclear pore complex protein Nup155 1 7,4 8 9 1 6,5 7 10 1 6,4 6 6 1 10,4 11 25 0,000198 167000069 TXNDC3 thioredoxin domain-containing protein 3 isoform 1 1 12,6 9 25 1 18,8 10 25 1 19,8 11 30 1 23,4 12 77 0,001087846 16716343 Gm16399 cytochrome c oxidase subunit 6C 1 46,1 5 15 1 69,7 6 15 1 46,1 5 15 1 69,7 6 45 0,004448522 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 167234369 NHEDC1 sodium/hydrogen exchanger 9B1 1 12,6 6 8 1 14,5 7 11 1 14,2 7 12 1 15,9 8 34 0,000667 167234411 NUBPL iron-sulfur protein NUBPL precursor 1 20,7 5 5 1 27 6 9 1 36,1 8 9 1 39,2 9 24 0,00059 cytochrome c oxidase subunit II [Mus musculus 167716840 COX2 musculus] 1 23,3 4 14 1 23,3 4 35 0 0 0 0 0 0 4 0 - 169646320 DPY19L1 probable C-mannosyltransferase DPY19L1 1 4,7 3 3 0 0 0 0 0 0 0 0 1 4,7 3 3 0,0000471 169808427 fucA1 tissue alpha-L-fucosidase precursor 1 10,6 4 5 1 11,9 5 5 1 10,6 4 5 1 13,9 6 15 0,000384 D-beta-hydroxybutyrate dehydrogenase, mitochondrial 170014720 BDH1 precursor 1 30,3 8 23 1 33,5 9 29 1 43,4 11 31 1 45,5 12 84 0,001483235 170650601 macrod1 O-acetyl-ADP-ribose deacetylase MACROD1 1 9,3 3 3 1 10,8 3 3 1 23,2 5 5 1 13,6 4 10 0,000208 mitochondrial import inner membrane translocase 170763467 Timm44 subunit TIM44 1 24,1 9 30 1 41,8 17 26 1 35,8 15 24 1 43,8 19 80 0,00103557 18079339 ACO2 aconitate hydratase, mitochondrial precursor 1 44,6 26 50 1 48,5 28 199 1 54,9 31 192 1 51,2 29 436 0,004188298 pyruvate dehydrogenase E1 component subunit beta, 18152793 Gm6123 mitochondrial precursor 1 53,2 13 97 1 52,4 13 189 1 63 16 194 1 63 16 473 0,007517306 isocitrate dehydrogenase [NAD] subunit alpha, 18250284 idh3a mitochondrial precursor 1 31,7 10 20 1 42,3 15 35 1 39,9 13 35 1 44,5 17 88 0,001598533 succinyl-CoA:3-ketoacid coenzyme A transferase 1, 18266680 oxct1 mitochondrial precursor 1 13,8 4 6 1 20,2 7 15 1 22,1 8 11 1 26 9 33 0,000431 183396771 Gm12141 60 kDa heat shock protein, mitochondrial 1 50,6 24 85 1 59,2 30 195 1 56,2 27 197 1 57,9 29 470 0,003763253 18390323 Rab14 ras-related protein Rab-14 1 78,6 13 34 1 71,2 12 30 1 82,8 16 48 1 82,8 16 112 0,002781134 18700024 IDH3B isocitrate dehydrogenase 3, beta subunit 1 36,2 12 31 1 37,8 12 17 1 47,4 14 38 1 49,7 15 88 0,001614965 188035922 ACRBP acrosin-binding protein isoform 1 precursor 1 26,3 11 34 1 30,4 12 65 1 30,4 12 57 1 30,4 12 160 0,003030314 188219602 INPP5A inositol polyphosphate-5-phosphatase A isoform b 1 12,1 5 10 1 19,9 8 8 1 21,1 8 10 1 23,8 9 27 0,000418 188528624 prmt5 protein arginine N-methyltransferase 5 1 11 5 5 1 14,1 6 7 1 15,4 7 7 1 17 8 19 0,000259 189409138 CAND1 cullin-associated NEDD8-dissociated protein 1 1 9,3 9 10 1 14,3 14 16 1 16 16 21 1 19,4 20 46 0,000298 tubulin polymerization-promoting protein family 191252810 TPPP2 member 2 1 22,9 3 6 1 28,2 4 7 1 32,9 5 10 1 32,9 5 23 0,000616

194097451 CATSPER4 cation channel sperm-associated protein 4 isoform 2 1 8,9 3 3 1 10 3 3 1 9,5 3 3 1 16,5 5 10 0,00025 NADH dehydrogenase [ubiquinone] flavoprotein 1, 19526814 Ndufv1 mitochondrial precursor 1 39,7 13 26 1 62,9 20 41 1 53 17 42 1 60,8 19 105 0,001594127 19526912 st13 hsc70-interacting protein 1 8,4 3 4 1 14,6 5 6 1 25,6 9 12 1 25,6 9 23 0,000544 19527102 STX12 syntaxin-12 1 27 6 8 1 24,8 5 7 1 20,4 4 7 1 33,2 7 24 0,000841 19527224 CSNK1G2 casein kinase I isoform gamma-2 isoform 1 1 11,1 4 9 0 0 0 0 0 0 0 0 1 18,6 7 23 0,00043 19527228 CISD1 CDGSH iron-sulfur domain-containing protein 1 1 51,9 5 8 1 52,8 6 13 1 52,8 6 12 1 52,8 6 35 0,002194542 methylmalonate-semialdehyde dehydrogenase 19527258 Aldh6a1 [acylating], mitochondrial precursor 1 12,5 5 6 1 35,3 14 24 1 28,4 11 29 1 39,3 15 58 0,000757 19705424 psmd3 26S proteasome non-ATPase regulatory subunit 3 1 12,1 5 8 1 11,3 5 6 1 15,8 7 9 1 24,2 11 23 0,000267 isochorismatase domain-containing protein 2A, 197333728 Isoc2a mitochondrial precursor 1 40,3 5 9 1 52,9 6 9 1 56,8 7 16 1 56,8 7 36 0,001910477 197927139 LOC630540 WD repeat domain 63 1 3,1 3 3 1 7 6 11 1 17,2 14 18 1 18,1 15 31 0,00031 198278567 cpvl probable serine carboxypeptidase CPVL precursor 1 14 6 11 1 13,2 6 7 1 12,3 5 9 1 16,5 7 28 0,000555 19882201 PSMD2 26S proteasome non-ATPase regulatory subunit 2 1 12,1 9 13 1 19,1 14 21 1 23,7 14 25 1 33 21 61 0,000559

209862989 Uba1 ubiquitin-like modifier-activating enzyme 1 isoform 2 1 3,5 3 4 1 9,1 7 8 1 15,7 12 15 1 15,7 12 25 0,00021 210031252 chdh choline dehydrogenase, mitochondrial 1 10,2 6 7 1 18,3 9 9 1 24,3 11 16 1 29 13 32 0,000434 21311859 Fam166a protein FAM166A 1 21 6 29 1 59,2 17 52 1 57,1 16 62 1 59,2 17 143 0,003265506 NADH dehydrogenase [ubiquinone] 1 alpha 21312012 Ndufa8 subcomplex subunit 8 1 30,8 4 7 1 64 8 11 1 59,3 7 16 1 64 8 34 0,001611923 21312181 LYZL4 lysozyme-like protein 4 precursor 1 33,8 3 4 1 33,8 3 4 1 33,8 3 4 1 33,8 3 12 0,000678 21312524 CYB5R1 NADH-cytochrome b5 reductase 1 1 11,8 3 3 1 13,4 3 3 0,9553 3,9 1 1 1 17,7 4 7 0,000196 21312594 Gm3982 mitochondrial pyruvate carrier 2 1 51,2 5 19 1 51,2 5 22 1 52 6 23 1 52 6 68 0,004985642 21312794 Pfn3 profilin-3 1 54,7 7 9 1 67,2 7 9 1 61,3 8 11 1 81 9 29 0,00113044 testis-specific serine/threonine-protein kinase 4 isoform 21312834 TSSK4 1 1 30,5 9 13 1 39,3 11 13 1 29,6 8 11 1 39,3 11 38 0,000701 21312844 lrrc48 leucine-rich repeat-containing protein 48 1 19,3 8 9 1 18 8 8 1 22,8 10 10 1 27,2 12 24 0,000374 sperm acrosome membrane-associated protein 1 21312846 SPACA1 precursor 1 23 5 87 1 23 5 89 1 32,8 7 101 1 31,1 6 278 0,011496927 21312922 CCDC39 coiled-coil domain-containing protein 39 1 11,7 10 11 1 14,5 12 16 1 17,6 14 20 1 19,1 16 47 0,000303 NADH dehydrogenase [ubiquinone] iron-sulfur protein 21312950 NDUFS7 7, mitochondrial precursor 1 21,9 6 23 1 21,9 6 20 1 21,4 5 21 1 21,9 6 64 0,002184117 21312974 RSPH9 radial spoke head protein 9 homolog 1 40,6 10 21 1 47,5 11 33 1 37 9 40 1 47,5 11 97 0,002326372 21312994 Slc25a11 mitochondrial 2-oxoglutarate/malate carrier protein 1 32,8 9 10 1 12,4 4 4 1 19,4 5 5 1 35,4 10 19 0,000379 21313012 RAB4B ras-related protein Rab-4B 1 28,2 5 7 1 17,4 3 4 1 33,3 6 8 1 33,3 6 19 0,000485 21313138 gstk1 glutathione S-transferase kappa 1 1 18,1 3 3 1 24,3 4 5 1 34,1 6 8 1 34,1 6 15 0,000392 dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, 21313536 DLST mitochondrial 1 24,4 8 34 1 22,5 8 16 1 27,1 9 26 1 27,1 9 76 0,001393699 21313544 BCS1L mitochondrial chaperone BCS1 1 17,9 6 11 0,9641 2,4 1 1 1 20,3 6 6 1 31,6 10 20 0,000353 coiled-coil-helix-coiled-coil-helix domain-containing 21313618 CHCHD3 protein 3, mitochondrial precursor 1 25,1 7 12 1 28,6 8 10 1 30,4 9 13 1 39,2 11 38 0,000947 21313679 LOC674469 ATP synthase subunit d, mitochondrial 1 74,5 11 23 1 67,7 9 19 1 73,9 10 31 1 74,5 11 70 0,003109368 21313683 DPEP3 dipeptidase 3 precursor 1 26,4 9 27 1 26,4 9 24 1 26,4 9 24 1 26,4 9 77 0,001421819 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 21314830 REEP6 receptor expression-enhancing protein 6 isoform 1 1 25,4 4 7 1 15,4 3 5 1 25,4 4 10 1 25,4 4 21 0,001100395 21314832 UGP2 UTP--glucose-1-phosphate uridylyltransferase 1 5,9 3 3 1 14,6 6 6 1 15,9 7 11 1 15,9 7 20 0,000259 21361209 Hist1h4c histone H4 1 50,5 5 5 1 40,8 4 5 1 51,5 6 8 1 51,5 6 18 0,000915 21362335 CPA5 carboxypeptidase A5 precursor 1 8,7 4 4 1 17,4 6 8 1 17,2 6 9 1 24,3 8 21 0,000481 214010170 LOC100046120clusterin precursor 1 9,8 4 17 1 7,1 3 6 1 15,8 6 15 1 15,8 6 38 0,000703

21450129 ACAT1 acetyl-CoA acetyltransferase, mitochondrial precursor 1 25 7 17 1 24,3 8 19 1 27,1 8 16 1 29 9 51 0,001027675 cat eye syndrome critical region protein 5 homolog 21450187 CECR5 precursor 1 28,9 9 27 1 40,8 12 24 1 36,3 11 19 1 39,1 12 69 0,00142685 21539585 UQCRQ cytochrome b-c1 complex subunit 8 1 28 3 5 1 28 3 7 1 28 3 6 1 28 3 19 0,001322319 21539599 Uqcrh cytochrome b-c1 complex subunit 6, mitochondrial 1 37,1 2 4 1 37,1 2 4 0,9994 20,2 1 2 1 44,9 3 11 0,000757 21553309 APOA1BP NAD(P)H-hydrate epimerase precursor 1 17,4 3 4 1 30,9 7 11 1 24,8 5 7 1 30,9 7 21 0,000583 dehydrogenase/reductase SDR family member 7B 21703854 DHRS7B isoform 2 1 25,8 6 9 1 18,5 5 5 1 23,6 5 6 1 26,1 7 19 0,000425 L-2-hydroxyglutarate dehydrogenase, mitochondrial 21703884 L2HGDH precursor 1 29,5 12 31 1 38,1 15 34 1 39,4 15 48 1 41,6 16 118 0,00153357

21703972 ME2 NAD-dependent malic enzyme, mitochondrial precursor 1 21,4 9 11 1 17 7 8 1 24,3 10 12 1 30,1 13 32 0,000431 bifunctional ATP-dependent dihydroxyacetone 21703976 dak kinase/FAD-AMP lyase (cyclizing) 1 42,2 15 43 1 37,5 16 37 1 45,5 18 61 1 49,3 19 141 0,001997272 trifunctional enzyme subunit beta, mitochondrial 21704100 Gm13910 precursor 1 42,3 16 48 1 54,1 18 72 1 45,9 18 53 1 59,2 21 173 0,003116362 3-hydroxyisobutyrate dehydrogenase, mitochondrial 21704140 hibadh precursor 1 40 9 18 1 57 11 32 1 50,1 11 32 1 50,1 11 81 0,001939088 21729773 1700001C02RikUPF0573 protein C2orf70 homolog 1 31,5 6 11 1 26 5 13 1 30,5 6 16 1 35 7 41 0,00141031 mitochondrial import inner membrane translocase 22094989 timm50 subunit TIM50 precursor 1 19,8 6 11 1 16,7 5 5 1 19,8 6 7 1 24,6 7 23 0,000559 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial 22122625 HIBCH precursor 1 15,8 5 8 1 22,9 7 7 1 22,9 7 9 1 25,5 8 25 0,000399 22128627 sorD sorbitol dehydrogenase 1 58 16 95 1 66,9 18 148 1 60,2 15 149 1 64,7 17 397 0,008220805 22165382 csnk1a1 casein kinase I isoform alpha 1 13,5 4 7 1 22,8 6 8 1 28 7 12 1 33,2 9 31 0,000627 22165384 TUBB2C tubulin beta-4B chain 1 76 25 97 1 79,8 26 120 1 76 25 103 1 76 25 303 0,003332613

22203753 PPA2 inorganic pyrophosphatase 2, mitochondrial precursor 1 11,2 3 3 1 17,6 5 8 1 19,4 5 6 1 23,3 6 17 0,00032 cytochrome b-c1 complex subunit 2, mitochondrial 22267442 Uqcrc2 precursor 1 44,6 17 145 1 49 18 120 1 49 18 143 1 49 18 402 0,007346425 223941805 Gm9047 uncharacterized protein LOC668210 1 26 5 8 1 16,4 3 10 1 17,5 3 8 1 30,5 6 25 0,000833 223972700 Fam71e2 uncharacterized protein LOC243822 1 7,8 5 8 1 6,8 4 4 1 11,5 6 12 1 12,9 7 26 0,000324 224809376 NCSTN nicastrin precursor 1 11,2 7 10 1 6,6 4 7 1 13,1 7 8 1 14,4 8 24 0,00034 glycerol-3-phosphate dehydrogenase, mitochondrial 224922803 Gpd2 precursor 1 25,2 15 17 1 59,7 35 126 1 61,3 32 138 1 65,6 35 273 0,001780131 225007547 GSTO2 glutathione S-transferase omega-2 1 15,7 3 3 1 31 8 15 1 28,2 7 11 1 31 8 32 0,001046446 22507355 SLC8A2 sodium/calcium exchanger 2 precursor 1 13,2 8 8 1 6,3 4 4 1 8,6 5 6 1 13,2 8 18 0,000231 cAMP-dependent protein kinase type II-alpha 22550094 PRKAR2A regulatory subunit 1 39,6 12 54 1 44,3 14 134 1 53 16 126 1 58,7 18 311 0,005355296 225543319 LOC675857 transitional endoplasmic reticulum ATPase 1 38,8 22 28 1 56,6 34 74 1 53,7 32 75 1 60,9 36 178 0,001460363 225543354 Zmynd12 zinc finger, MYND domain containing 12 1 24 6 10 1 20,9 5 5 1 33,1 9 13 1 37,5 10 28 0,000517 225543576 gtl3 UPF0468 protein C16orf80 homolog 1 31,6 7 15 1 48,2 9 13 1 43,5 7 16 1 48,2 9 43 0,001469589 iron-sulfur cluster co-chaperone protein HscB, 225703114 hscB mitochondrial precursor 1 29,1 5 8 1 25,2 5 8 1 32,9 6 8 1 32,9 6 24 0,000571 225703118 Ado 2-aminoethanethiol dioxygenase 1 14,5 3 3 1 23,8 5 5 1 20,7 4 4 1 23,8 5 12 0,000357 225735582 HK1 hexokinase-1 isoform HK1-sb 1 46,9 38 216 1 50,9 42 424 1 49,6 40 371 1 54,4 43 989 0,005196722 226246531 PGK2 phosphoglycerate kinase 2 1 54,9 16 112 1 67,4 20 123 1 65,7 20 131 1 69,5 22 374 0,00600817 226442909 NIPSNAP3A nipsnap homolog 3A 1 34,7 7 14 1 25,7 5 14 1 25,7 5 18 1 34,7 7 47 0,001875618 226442916 spata18 mitochondria-eating protein 1 14 6 15 1 17,1 8 54 1 19,2 8 55 1 20,7 9 124 0,002173621 226442984 PTCHD3 patched domain-containing protein 3 1 15,3 10 15 1 19,3 14 25 1 18,4 12 21 1 19,2 13 59 0,000893 226443015 Faah fatty-acid amide hydrolase 1 1 5,2 3 3 1 5,7 3 3 0,9995 3,3 2 2 1 9,3 5 8 0,000139 226443038 Glipr1l3 GLI pathogenesis-related 1 like 1 precursor 1 27,1 6 9 1 27,1 6 7 1 32,6 7 11 1 32,6 7 29 0,000998

226530884 pcmt1 protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 13,3 3 4 0,9999 9,8 2 3 1 21,4 4 5 1 24,9 5 12 0,000317 226530999 DNAJB3 dnaJ homolog subfamily B member 3 1 12,8 3 3 0,9997 9,5 2 2 1 16,9 4 4 1 16,9 4 9 0,000263 226823367 Fh1 fumarate hydratase, mitochondrial precursor 1 23,1 7 18 1 38,3 11 21 1 21,7 7 12 1 40,2 12 50 0,000815 226958349 TPI1 triosephosphate isomerase 1 29,1 7 10 1 52,5 13 29 1 52,2 12 35 1 52,2 12 72 0,001421865

226958351 ATP1A4 sodium/potassium-transporting ATPase subunit alpha-4 1 34,8 31 71 1 40,5 35 126 1 39,7 34 97 1 41,3 36 253 0,002111607 226958374 Sypl synaptophysin-like protein 1 isoform 1 1 18,9 3 4 0,9997 14 2 5 0,9991 14 2 7 1 18,9 3 16 0,000775 227116345 psmb2 proteasome subunit beta type-2 1 16,4 3 6 1 32,3 5 9 1 47,8 6 11 1 51,7 7 28 0,000936

227116358 MECR trans-2-enoyl-CoA reductase, mitochondrial precursor 1 31,1 9 20 1 48,3 12 18 1 51,5 13 17 1 51,5 13 55 0,001161125 227330582 G6pd2 glucose-6-phosphate 1-dehydrogenase 2 1 14,8 7 8 1 15,2 8 4 1 21,6 10 7 1 23,8 11 15 0,000214 227330593 Dnaic1 dynein intermediate chain 1, axonemal 1 19,1 10 18 1 30,1 17 38 1 34,1 18 31 1 39,2 22 81 0,000856 227330625 MAN2B2 epididymis-specific alpha-mannosidase precursor 1 16 13 26 1 16,1 11 37 1 25,3 18 43 1 25,3 19 107 0,001273011 227496048 Gm5347 a disintegrin and metallopeptidase domain 34 1 9,8 6 10 1 9 5 11 1 7,3 4 6 1 11,5 7 26 0,000412 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 227908775 RSPH6A radial spoke head protein 6 homolog A isoform 1 1 11,4 5 14 1 10,7 5 19 1 16,1 7 30 1 19,4 8 62 0,001295658 228480241 MTX2 metaxin-2 1 20,2 4 9 1 33,8 6 9 1 28,5 5 7 1 35 6 24 0,000991 sperm acrosome membrane-associated protein 4 228480264 Spaca4 precursor 1 40,9 4 6 1 40,9 4 10 1 33,1 3 6 1 40,9 4 23 0,001723134 zona pellucida sperm-binding protein 3 receptor 229577240 Zp3r precursor 1 12,4 5 23 1 23,3 10 49 1 19 9 48 1 22,1 10 117 0,002057406 ubiquitin carboxyl-terminal hydrolase isozyme L5 229577281 UCHL5 isoform 1 1 10,7 3 5 1 17,7 5 5 1 31,1 8 8 1 36,3 9 18 0,00042 229608935 NME7 nucleoside diphosphate kinase 7 isoform 1 1 8,6 3 4 1 33,2 10 12 1 38,5 10 16 1 43,8 12 31 0,000571 NADH-ubiquinone oxidoreductase 75 kDa subunit, 229892316 NDUFS1 mitochondrial precursor 1 38,2 21 35 1 53,4 29 113 1 49,8 27 112 1 57,8 32 264 0,002458881 NADH dehydrogenase [ubiquinone] iron-sulfur protein 23346461 ndufs2 2, mitochondrial precursor 1 30 13 29 1 33 12 21 1 33,7 14 36 1 33,7 14 84 0,001556297 23346525 CD109 CD109 antigen precursor 1 12,9 15 27 1 16,5 16 32 1 14,4 17 35 1 22,2 23 94 0,000649 23346533 PHOSPHO1 phosphoethanolamine/phosphocholine phosphatase 1 22,8 6 12 1 23,2 7 14 1 33 9 19 1 36 11 46 0,001129927 23346557 oplaH 5-oxoprolinase 1 6,4 7 7 1 3,6 4 4 1 12,9 11 11 1 15,8 14 21 0,000156 amyotrophic lateral sclerosis 2 (juvenile) chromosome 237649035 Als2cr11 region, candidate 11 1 11 5 16 0,9996 3,6 2 3 0,9996 3,6 2 4 1 11 5 23 0,000328 238231384 PSMB6 proteasome subunit beta type-6 precursor 1 16,8 4 6 1 12,6 3 3 1 16 3 5 1 23,9 5 14 0,000442

238550131 Efcab9 EF-hand calcium-binding domain-containing protein 9 1 9,7 2 5 0,9994 9,7 2 2 0 0 0 0 1 9,7 2 7 0,000295 acyl-coenzyme A thioesterase 2, mitochondrial 238624114 Acot2 precursor 1 6,9 3 4 1 10 4 4 0 0 0 0 0 0 6 0 - 238814391 Cct7 T-complex protein 1 subunit eta 1 20,4 9 12 1 22,6 11 24 1 38,2 18 29 1 38,2 18 63 0,000726 EF-hand calcium-binding domain-containing protein 6 239052655 EFCAB6 isoform 1 1 15,3 11 18 1 30 20 69 1 26,8 17 52 1 27,8 18 134 0,000564 23943818 ccdc65 coiled-coil domain-containing protein 65 1 18,9 7 7 1 8,5 4 4 1 17,4 8 9 1 24,1 10 21 0,00027 phosphatidylglycerophosphatase and protein-tyrosine 23956130 ptpmt1 phosphatase 1 1 26,1 7 11 1 23,8 5 6 1 32,6 8 13 1 32,6 8 30 0,000708 23956396 Erlin2 erlin-2 1 22,4 6 16 1 33,8 9 9 1 37,1 10 16 1 40 11 43 0,000829 240120054 Akr7a5 aflatoxin B1 aldehyde reductase member 2 1 21,5 7 12 1 21,5 7 9 1 15,8 4 10 1 24,8 8 31 0,000856 242247109 CYLC2 cylicin, basic protein of sperm head cytoskeleton 2 1 11,1 3 21 1 8,3 2 4 1 16,1 5 11 1 16,1 5 37 0,000612 242397497 1700012P22Rikuncharacterized protein C1orf158 homolog 1 21,9 4 6 1 45,4 8 11 1 51,5 9 12 1 51,5 9 28 0,00093 244790045 SQRDL sulfide:quinone oxidoreductase, mitochondrial 1 26,7 10 28 1 26,2 11 16 1 34,7 14 21 1 35,1 17 67 0,000845 244791124 DPP3 dipeptidyl peptidase 3 1 7 3 3 1 12,9 7 7 1 15,6 7 7 1 19 10 18 0,000181 25072207 Dynlrb2 dynein light chain roadblock-type 2 1 60,4 4 6 1 60,4 4 6 1 60,4 4 7 1 60,4 4 19 0,001193492 251823722 1700023D19Rikizumo sperm-egg fusion protein 2 precursor 1 21,2 4 10 1 16,6 3 6 1 16,6 3 7 1 21,2 4 23 0,000932 253795525 CCDC147 coiled-coil domain containing 147 1 27,9 23 36 1 21,4 16 25 1 27,7 21 36 1 29,9 25 100 0,000649 253795534 FSCB fibrous sheath CABYR-binding protein 1 15 11 24 1 18,5 14 61 1 19,3 15 64 1 20,1 16 147 0,00234733 254281222 HTRA2 serine protease HTRA2, mitochondrial 1 33,8 10 20 1 36,5 11 16 1 29,7 10 15 1 42,6 12 52 0,000954 254540027 Mdh1 malate dehydrogenase, cytoplasmic 1 17,1 5 5 1 25,1 7 8 1 26 7 8 1 28,1 8 20 0,000414 254540082 PSMB4 proteasome subunit beta type-4 precursor 1 25,4 4 6 1 33 5 9 1 42 6 14 1 42 6 29 0,000917 254540166 HSPA5 78 kDa glucose-regulated protein precursor 1 25,2 13 11 1 33,3 17 19 1 29,5 16 26 1 34,8 19 57 0,000556 254553303 DYDC1 DPY30 domain-containing protein 1 1 35,4 6 11 1 30,3 5 5 1 30,9 5 8 1 35,4 6 24 0,000873 254553344 PFKM 6-phosphofructokinase, muscle type 1 12,6 8 7 1 19,9 13 13 1 27,7 17 21 1 27,6 17 39 0,000401 254553376 CCDC105 coiled-coil domain-containing protein 105 1 25,5 11 28 1 40,5 19 55 1 32,1 14 55 1 37,1 17 141 0,001754358 254553422 EFHC1 EF-hand domain-containing protein 1 1 21 11 12 1 50,3 23 50 1 49,8 23 55 1 53,1 24 111 0,001108667 254553434 IL4I1 L-amino-acid oxidase precursor 1 19 10 30 1 26,2 13 47 1 36,3 17 57 1 36,3 17 133 0,001965055 254553458 gpi1 glucose-6-phosphate isomerase 1 29,9 13 29 1 40,7 18 75 1 47 19 62 1 46,1 20 170 0,002862517 254587947 ppp2r4 serine/threonine-protein phosphatase 2A activator 1 10,2 3 5 1 11,1 3 3 1 13,6 4 6 1 13,6 4 13 0,000269 serine/threonine-protein phosphatase PGAM5, 254587960 pgam5 mitochondrial isoform 2 1 44,3 11 15 1 38 9 18 1 43,9 10 23 1 44,3 11 58 0,00118842 254675102 RTDR1 rhabdoid tumor deletion region gene 1 isoform 1 1 10,3 3 4 1 9,7 3 4 1 10,3 3 5 1 14,1 4 14 0,000247 254675124 Ccdc19 nasopharyngeal epithelium specific protein 1 1 5,6 3 12 1 17,1 8 26 1 13,2 6 24 1 15,2 7 61 0,000664 254675150 hspb9 heat shock protein beta-9 1 31,7 6 11 1 36,2 7 18 1 52,3 9 24 1 52,3 9 53 0,001599544 uncharacterized coiled-coil domain-containing protein 254675160 4921530D09RikKIAA1984 1 12,9 6 14 1 22,1 10 22 1 24,5 11 25 1 29,2 13 61 0,000675 254675164 Tmem190 transmembrane protein 190 precursor 1 15,1 2 11 1 23,5 3 7 1 23,5 3 10 1 23,5 3 27 0,002222989 254675166 ak7 adenylate kinase 7 1 21,6 13 23 1 20,3 11 26 1 20,7 12 25 1 26,3 16 74 0,000794 254675236 KIF9 kinesin-like protein KIF9 isoform 2 1 9,9 5 5 0,9983 2 2 2 0,9988 3,8 2 2 1 12,3 7 8 0,0000769 254675347 glb1l beta-galactosidase-1-like protein precursor 1 5,1 3 3 1 12,1 6 7 1 17,3 9 9 1 17,3 9 18 0,000225 254692831 PSMA8 proteasome subunit alpha type-7-like 1 35,2 6 8 1 35,6 7 5 1 35,6 7 11 1 38,8 8 34 0,000812 NADH dehydrogenase [ubiquinone] 1 alpha 254692859 NDUFA9 subcomplex subunit 9, mitochondrial precursor 1 26 9 27 1 23,6 8 21 1 28,9 9 23 1 31,6 11 70 0,001325477 254692892 Slc25a31 ADP/ATP translocase 4 1 33,1 11 47 1 35,3 12 58 1 35,3 12 60 1 35,3 12 151 0,002373479 254692940 TTC18 tetratricopeptide repeat domain 18 isoform 3 1 2,6 3 4 1 7,7 7 11 1 8,4 8 14 1 10,6 10 31 0,000265 254826790 slc25a10 mitochondrial dicarboxylate carrier 1 25,8 7 14 1 29,6 7 7 1 34,8 9 14 1 37,6 10 36 0,000906 254826802 4930511I11Rik armadillo repeat-containing protein 12 1 10 2 7 1 29,4 7 17 1 17,6 4 13 1 26,8 6 37 0,001218772 254910995 Naglu alpha-N-acetylglucosaminidase precursor 1 23 13 20 1 26,5 16 31 1 24,2 15 22 1 28,1 17 73 0,000903 254911018 ATP6V1E2 V-type proton ATPase subunit E 2 1 22,1 5 6 1 11,9 3 3 1 22,6 5 5 1 26,1 6 14 0,000365 255003777 GLOD4 glyoxalase domain-containing protein 4 1 21,1 5 6 1 54 13 19 1 51,3 12 20 1 51,3 12 43 0,00077 255069715 LAP3 cytosol aminopeptidase 1 38,5 15 23 1 47 20 63 1 45,7 18 57 1 47,4 20 142 0,001714935 255683347 CCDC151 coiled-coil domain-containing protein 151 isoform 2 1 24,6 13 16 1 16,4 8 16 1 20,2 10 17 1 28 15 50 0,000494 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX succinyl-CoA ligase [ADP/GDP-forming] subunit 255958286 suclg1 alpha, mitochondrial precursor 1 35,3 10 71 1 37,9 11 102 1 38,2 12 103 1 38,2 12 276 0,00943364 256000803 1700006E09Rikuncharacterized protein C17orf105 homolog 1 29,3 4 6 1 23,2 3 6 1 29,3 4 12 1 29,3 4 23 0,000897 dehydrogenase/reductase SDR family member 4 256220343 Dhrs4 isoform 1 1 40,1 9 15 1 34,8 8 11 1 40,9 10 14 1 43,7 11 40 0,000867 256665241 zan zonadhesin precursor 1 18,5 71 146 1 23,2 88 264 1 25,8 95 240 1 26,5 98 658 0,001237238 256818776 Wdr52 WD repeat-containing protein 52 1 5,9 8 13 1 7,8 12 17 1 7,8 11 20 1 10,4 15 52 0,000256 acyl-Coenzyme A dehydrogenase family, member 10- 257467675 9330129D05Riklike 1 24,1 12 27 1 31,4 13 17 1 22,9 12 26 1 32,6 14 70 0,000901 257743039 LdhA L-lactate dehydrogenase A chain isoform 2 1 26 8 25 1 33 12 52 1 29,1 11 53 1 33 12 131 0,001959904

dihydrolipoyllysine-residue acetyltransferase component 257796245 dlaT of pyruvate dehydrogenase complex, mitochondrial 1 44,9 21 101 1 52,6 25 225 1 49,4 24 224 1 55 26 547 0,005934628 cation channel sperm-associated protein subunit beta 257900479 CATSPERB precursor 1 14,1 13 17 1 3,7 4 6 1 3,9 4 4 1 14,9 14 28 0,000217 260064069 WDR65 WD repeat-containing protein 65 1 18,1 19 25 1 28 27 58 1 28,4 26 48 1 32,4 31 129 0,000705 maestro heat-like repeat-containing protein family 260593706 Heatr7b2 member 2B 1 11,2 15 26 1 14,5 20 37 1 16,3 21 37 1 18 24 102 0,000486 cation channel sperm-associated protein subunit delta 261245039 TMEM146 precursor 1 9,1 5 9 1 9,9 5 5 1 10,3 5 5 1 13 7 19 0,000289 261399871 CCDC33 coiled-coil domain-containing protein 33 isoform 1 1 5,5 3 4 0 0 0 0 0 0 0 0 1 7,5 4 5 0,0000421 261824000 Psma3 proteasome subunit alpha type-3 1 28,6 6 10 1 28,2 6 10 1 26,7 6 10 1 31,4 8 32 0,000726 solute carrier family 2, facilitated glucose transporter 261862282 SLC2A3 member 3 1 13,8 6 29 1 14 7 64 1 14 7 54 1 14 7 148 0,004831955 261862311 CCDC11 coiled-coil domain-containing protein 11 1 9,9 4 5 1 19,1 8 8 1 21,6 9 12 1 21,6 9 27 0,000308 262050574 EFHC2 EF-hand domain-containing family member C2 1 6,4 4 7 1 27,1 17 35 1 17,6 11 27 1 29,2 18 67 0,000645

262072996 ZPBP2 zona pellucida-binding protein 2 isoform 1 precursor 1 38 10 39 1 36,2 11 48 1 34,4 11 53 1 38,7 12 144 0,003500504 262359961 1700101E01Rikuncharacterized protein LOC329375 isoform 1 1 18,1 4 5 1 21,8 5 12 1 18,1 4 9 1 21,8 5 28 0,000676 coiled-coil-helix-coiled-coil-helix domain-containing 267844804 CHCHD6 protein 6, mitochondrial isoform 1 1 29,8 7 12 1 42 8 19 1 47,8 11 23 1 49 12 52 0,00148473 268370161 1700026D08Rikuncharacterized protein C15orf26 homolog 1 28,7 6 7 1 35 8 14 1 31,7 7 15 1 35 8 36 0,000919 268370191 NT5C1B cytosolic 5'-nucleotidase 1B 1 31,8 14 75 1 49,2 22 135 1 40,7 17 140 1 49,2 22 346 0,004628911 268370211 Rnls renalase isoform 1 1 48,7 8 34 1 48,7 8 42 1 48,7 8 44 1 48,7 8 125 0,004596706 268370275 Gm884 uncharacterized protein LOC380730 1 14,7 19 53 1 12,3 17 82 1 13,4 18 76 1 14,7 19 215 0,001421706 269784615 atp2b4 plasma membrane calcium ATPase 4 isoform a 1 25 24 32 1 25,6 25 70 0,9996 25,6 25 7 1 25,6 25 18 0,000143 269784687 Slc25a21 mitochondrial 2-oxodicarboxylate carrier isoform 1 1 17,4 5 6 0 0 0 0 0,9842 4,4 1 2 1 17,4 5 8 0,000165 mycophenolic acid acyl-glucuronide esterase, 269784760 ABHD10 mitochondrial isoform 1 precursor 1 19,5 6 15 1 28,8 8 13 1 28,1 8 13 1 31,2 9 42 0,000861 269784762 cbr4 carbonyl reductase family member 4 1 30,5 6 12 1 33,5 6 17 1 46,6 8 22 1 37,3 7 50 0,001357221 269973935 Csl citrate synthase-like protein 1 47,9 17 115 1 47,9 17 140 1 47,9 17 159 1 47,9 17 411 0,006476897 27229101 5730469M10Rikredox-regulatory protein FAM213A 1 15,6 3 3 0,9805 6 1 1 0,9993 11,9 2 2 1 21,6 4 7 0,000198 27369533 LOC100039128nuclear pore complex protein Nup93 1 9,5 7 9 1 11,2 8 8 1 17,2 9 9 1 27,2 17 26 0,000205 probable isocitrate dehydrogenase [NAD] gamma 2, 27370380 4933405O20Rikmitochondrial 1 19,2 6 11 1 28,8 8 11 1 19,9 6 14 1 30,8 9 36 0,000753 27370404 CCDC113 coiled-coil domain-containing protein 113 1 12,7 4 8 0,9998 6,1 2 4 0,9859 3,7 1 2 1 12,7 4 14 0,000246 27734998 Tmem11 transmembrane protein 11, mitochondrial isoform 1 1 17,6 3 3 0,9985 13,6 2 2 1 27,3 5 7 1 27,3 5 14 0,000671 27754007 NDUFAB1 acyl carrier protein, mitochondrial precursor 1 14,7 2 3 1 15,4 3 4 0,9635 5,8 1 1 1 15,4 3 8 0,000413 27754103 Psmc6 26S protease regulatory subunit 10B 1 15,7 5 9 1 9 3 5 1 25,2 7 10 1 28,3 8 23 0,000361 NADH dehydrogenase [ubiquinone] 1 beta subcomplex 27754144 Ndufb5 subunit 5, mitochondrial precursor 1 16,9 3 6 1 23,8 4 7 1 28 5 11 1 28 5 23 0,000841

27923590 Adam4 a disintegrin and metallopeptidase domain 4 precursor 1 10,1 7 8 1 11,3 8 7 1 11,3 8 9 1 11,3 8 24 0,000261 28173550 39326 septin-7 isoform 1 1 31,4 10 15 1 22,2 8 9 1 37,8 11 16 1 39,6 12 41 0,000579 pyruvate dehydrogenase protein X component, 28201978 LOC100044117mitochondrial 1 30,3 11 30 1 30,3 11 55 1 30,3 11 60 1 30,3 11 145 0,002185123 ankyrin repeat and EF-hand domain-containing protein 28316764 ANKRD5 1 1 8 5 7 1 9,2 6 9 1 7,4 4 5 1 15,1 10 22 0,000174 283837762 Dnahc17 dynein heavy chain 17, axonemal 1 1,7 7 12 1 24 85 138 1 16,8 58 93 1 25,4 89 241 0,000404 29243946 Thnsl1 threonine synthase-like 1 isoform a 1 13,9 9 10 1 32,1 18 23 1 29,5 17 20 1 37,8 21 54 0,000532

29244434 BPI bactericidal permeability-increasing protein precursor 1 8,4 3 3 1 10,3 4 7 1 8,4 3 5 1 8,4 3 14 0,000249

293597567 Aldoa fructose-bisphosphate aldolase A isoform 1 precursor 1 63,4 19 44 1 83,5 26 57 1 74,2 22 52 1 81,8 24 148 0,001656066 fumarylacetoacetate hydrolase domain-containing 29366814 Fahd2a protein 2A 1 51,1 12 23 1 51,1 12 37 1 51,1 12 40 1 51,1 12 100 0,002582043 295317325 snap23 synaptosomal-associated protein 23 isoform a 1 15,7 3 6 0,9997 12,9 2 2 1 18,1 3 3 1 30 5 11 0,000281 295444814 Prss46 serine protease 46 1 17,2 5 22 1 17,2 5 18 1 22,3 6 21 1 22,3 6 61 0,00210585 295789144 - UPF0704 protein C6orf165 homolog 1 9,7 5 5 1 16,1 8 10 1 9,7 5 5 1 19,4 10 20 0,000305 29648322 DNAJB13 dnaJ homolog subfamily B member 13 1 29,4 6 11 1 29,4 7 19 1 29,7 8 21 1 29,7 8 51 0,000956 297747362 zpbp1 zona pellucida-binding protein 1 isoform 2 1 35,9 12 58 1 55,6 16 98 1 59,3 15 93 1 61,5 17 255 0,006304115 NADH dehydrogenase [ubiquinone] 1 beta subcomplex 29789148 NDUFB9 subunit 9 1 40,2 5 7 1 45,3 6 8 1 50,3 6 10 1 54,2 7 26 0,001179033 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 29789217 TSNAXIP1 translin-associated factor X-interacting protein 1 1 8,1 5 5 0,9998 3,6 2 3 1 11,5 7 9 1 16,2 10 19 0,000168 29789289 Echs1 enoyl-CoA hydratase, mitochondrial precursor 1 25,2 6 7 1 26,6 6 7 1 32,8 8 9 1 32,8 8 23 0,000558 298231198 Nup53 nucleoporin NUP53 isoform 2 1 19,5 4 6 0,9895 6,2 1 1 0,9998 8,8 2 2 1 23,4 5 9 0,000307 ATP-dependent (S)-NAD(P)H-hydrate dehydratase 298676450 Carkd isoform 1 1 26 5 5 1 14,7 3 4 1 17,4 4 6 1 36,4 8 16 0,000314 30172570 DNALI1 axonemal dynein light intermediate polypeptide 1 1 55 13 31 1 55,4 14 38 1 55,4 14 37 1 55,4 14 107 0,002401976 30410018 Iqcg IQ domain-containing protein G 1 7,6 3 6 1 7,6 3 9 1 10,5 4 9 1 10,5 4 25 0,000458 30424705 Tmem177 transmembrane protein 177 1 14,5 5 5 1 8,7 3 3 1 11,9 4 4 1 14,5 5 12 0,000332 30425048 LDHAL6B L-lactate dehydrogenase A-like 6B 1 44,8 11 91 1 52,9 13 139 1 58,4 14 140 1 58,4 14 370 0,005257715 30425148 CCDC40 coiled-coil domain-containing protein 40 1 12,8 12 16 1 9,8 9 19 1 13,4 12 18 1 17,2 16 55 0,000378 30425270 FAM78A protein FAM78A 1 14,1 3 4 1 14,1 3 3 0,9997 9,9 2 3 1 14,1 3 10 0,00035 sorting and assembly machinery component 50 30519943 Samm50 homolog 1 34,3 12 13 1 28,1 10 17 1 29 11 23 1 43,1 14 53 0,000806 30520019 sccpdh saccharopine dehydrogenase-like oxidoreductase 1 31,5 9 46 1 63,9 16 84 1 55,7 15 83 1 67,1 18 209 0,003928832 NAD-dependent protein deacylase sirtuin-5, 30578432 SIRT5 mitochondrial 1 13,2 3 3 1 18,1 5 5 1 24,2 5 5 1 28,7 7 14 0,000313 307344688 Wdr96 WD repeat domain 96 1 4,3 6 8 1 8 10 13 1 8,7 11 17 1 11,5 15 39 0,000197 30794150 man2c1 alpha-mannosidase 2C1 1 15,1 12 22 1 22,3 16 22 1 21,8 16 23 1 28,1 20 67 0,000638 cAMP-dependent protein kinase type I-alpha regulatory 30794476 PRKAR1A subunit 1 20,5 6 13 1 22,6 7 12 1 20,5 6 8 1 23,4 7 36 0,000713 30841008 RAB18 ras-related protein Rab-18 isoform 2 precursor 1 17 3 3 0,9856 6,3 1 1 1 24,3 4 4 1 24,3 4 8 0,000199 PREDICTED: adenylate kinase domain-containing 309262133 Akd1 protein 1 1 4 7 9 0,9999 1,2 2 4 1 4,3 7 9 1 6,7 11 24 0,0001 PREDICTED: NADH dehydrogenase [ubiquinone] iron- 309265938 Ndufs5 sulfur protein 5-like 1 34,9 3 4 1 41,5 4 7 1 34,9 3 5 1 41,5 4 16 0,00077 PREDICTED: LOW QUALITY PROTEIN: dynein 309268955 dnhc3 heavy chain 3, axonemal 1 0,5 3 3 1 12 41 47 1 12,5 38 40 1 16,1 52 89 0,000163 312222726 Angel1 protein angel homolog 2 isoform 2 1 7,5 3 5 1 16,7 6 6 1 14,4 6 6 1 20,1 7 17 0,000266 voltage-dependent anion-selective channel protein 3 312222784 Vdac3 isoform 1 1 27,2 5 6 0 0 0 0 0 0 0 0 0 0 10 0 - 312922382 aldoa1a aldolase 1 A retrogene 1 1 32,7 12 8 1 54,2 17 18 1 58,5 16 16 1 60,9 17 46 0,000618 31542427 LOC677611 casein kinase II subunit alpha 1 26,6 7 15 1 26,9 7 9 1 22,5 6 11 1 34,3 9 37 0,00087 31542438 LOC100047577cytochrome b5 type B precursor 1 45,2 4 11 1 21,9 3 6 1 63,7 7 19 1 63,7 7 35 0,002030367 solute carrier family 2, facilitated glucose transporter 31543728 SLC2A5 member 5 1 8,2 4 8 1 8,2 4 13 1 8,2 4 16 1 8,4 5 38 0,001433678

31543773 SSSCA1 Sjoegren syndrome/scleroderma autoantigen 1 homolog 1 31,2 4 6 1 31,2 4 4 1 31,2 4 5 1 31,2 4 15 0,000694 31560090 PLBD2 putative phospholipase B-like 2 precursor 1 5,7 3 6 1 20 8 10 1 21,5 9 10 1 24,1 10 26 0,000448 31560239 ASRGL1 isoaspartyl peptidase/L-asparaginase 1 40,2 10 17 1 48,2 12 38 1 44,5 11 36 1 48,2 12 89 0,001535239 31560255 Fam82b regulator of microtubule dynamics protein 1 1 17,7 4 4 1 23,9 6 6 1 23,9 6 7 1 23,9 6 18 0,000417 31560590 Fhl4 four and a half LIM domains 4 1 16,1 4 7 1 41,6 8 14 1 30,8 7 15 1 41,6 8 36 0,000894 31560686 Hspa2 heat shock-related 70 kDa protein 2 1 29,9 15 17 1 38,2 20 33 1 33,6 18 35 1 43,8 22 87 0,000839 31560705 ACSL1 long-chain-fatty-acid--CoA ligase 1 1 29,9 20 62 1 39,2 26 90 1 37,6 21 88 1 38,2 25 238 0,002442966 31560731 Atp6v1a V-type proton ATPase catalytic subunit A 1 10,4 5 6 1 20,3 10 12 1 11,5 6 7 1 20,3 10 24 0,000271

31980648 ATP5B ATP synthase subunit beta, mitochondrial precursor 1 71,6 23 292 1 68,6 22 322 1 71,6 23 325 1 71,6 23 948 0,009936516 31980744 Gm4045 ATP synthase subunit g, mitochondrial 1 40,8 3 7 1 49,5 4 7 1 40,8 3 8 1 40,8 3 21 0,001624239

31980762 Sod2 superoxide dismutase [Mn], mitochondrial precursor 1 22,5 4 12 1 43,7 6 20 1 40,5 5 17 1 43,7 6 48 0,001968067 31980844 dhrs1 dehydrogenase/reductase SDR family member 1 1 11,8 3 3 1 11,5 3 3 0,9813 3,8 1 1 1 11,8 3 7 0,000138 31980939 1700020C11Rikmitochondrial fission process protein 1 1 21,7 4 6 1 27,7 4 4 1 27,7 4 5 1 28,3 5 15 0,000655 31980942 IMPA1 inositol monophosphatase 1 1 30,7 7 9 1 31,8 9 12 1 40,1 10 12 1 48,7 12 33 0,000763 31980998 acot9 acyl-coenzyme A thioesterase 9, mitochondrial 1 37,8 16 41 1 46,5 17 58 1 44,2 16 79 1 50,6 19 180 0,002592449 NADH dehydrogenase [ubiquinone] 1 alpha 31981600 Ndufa2 subcomplex subunit 2 1 48,5 5 6 1 48,5 5 6 1 41,4 4 5 1 48,5 5 17 0,000807 31981690 LOC641192 heat shock cognate 71 kDa protein 1 15,9 9 8 1 16,9 9 13 1 14,6 8 12 1 18,7 10 35 0,000352

31981810 dci enoyl-CoA delta isomerase 1, mitochondrial precursor 1 16,6 4 8 1 19,7 5 7 1 16,6 4 6 1 19,7 5 21 0,000513 cytochrome c oxidase subunit 7A2, mitochondrial 31981830 COX7A2 precursor 1 27,7 2 5 1 27,7 2 6 1 27,7 2 4 1 27,7 2 16 0,00145053 31981922 LOC100048601probable threonine protease PRSS50 precursor 1 8,7 3 9 1 8,7 3 5 1 10,5 4 8 1 10,5 4 22 0,000591

31982091 Gm12906 mitochondrial import receptor subunit TOM22 homolog 1 33,1 3 4 1 31 3 5 1 33,1 3 7 1 47,9 4 16 0,001093137 31982186 mdh2 malate dehydrogenase, mitochondrial precursor 1 52,7 14 48 1 58,3 15 110 1 58,3 15 95 1 58,3 15 250 0,004574706 31982332 GLUL glutamine synthetase 1 16,9 6 13 1 25,5 10 21 1 28,2 10 27 1 30 11 61 0,001431574 31982710 FSCN3 fascin-3 1 9,8 4 5 0,9871 1,6 1 2 0,9994 4,2 2 2 1 9,8 4 9 0,000186

31982856 dld dihydrolipoyl dehydrogenase, mitochondrial precursor 1 39,1 12 25 1 40,7 13 90 1 58 16 104 1 59,5 17 220 0,003469746 33147082 bat3 large proline-rich protein BAG6 isoform 1 1 19,5 14 21 1 7 6 14 1 16,8 13 18 1 23,2 17 56 0,000769 33354251 Lypd4 ly6/PLAUR domain-containing protein 4 precursor 1 37,4 7 17 1 42,7 7 25 1 31,3 6 19 1 48,8 8 66 0,002375705 334688818 Wdr78 WD repeat-containing protein 78 1 7,1 5 5 1 7,7 6 7 1 9,9 7 10 1 12,8 9 21 0,000192 33469015 prcp lysosomal Pro-X carboxypeptidase precursor 1 13 7 8 1 17,7 8 12 1 17,7 8 11 1 22,2 12 30 0,000644 33563282 PSMA1 proteasome subunit alpha type-1 1 36,1 10 12 1 36,1 10 13 1 52,1 11 12 1 64,3 14 35 0,001014368 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 336088630 Nit1 nitrilase homolog 1 isoform 2 1 36,2 8 8 1 43,4 9 14 1 43,4 10 11 1 57,2 12 31 0,000764 33859530 cryz quinone oxidoreductase 1 24,8 8 20 1 31,4 10 14 1 41,7 11 14 1 41,7 11 50 0,001038221 33859604 PSMC2 26S protease regulatory subunit 7 1 16,8 7 11 1 18,1 7 7 1 19,2 8 8 1 26,5 11 25 0,000264 33859638 THEG testicular haploid expressed gene protein isoform 1 1 27,4 9 32 1 27,7 11 30 1 23,7 9 31 1 32,5 12 92 0,001899424 trifunctional enzyme subunit alpha, mitochondrial 33859811 HADHA precursor 1 31,3 19 30 1 58,5 31 112 1 55,7 30 115 1 56,6 31 253 0,002128039 34303985 CCDC63 coiled-coil domain-containing protein 63 1 23,5 14 29 1 19,1 10 34 1 19,2 12 35 1 25,1 15 100 0,001076706 34328230 Ak2 adenylate kinase 2, mitochondrial isoform b 1 38,8 8 21 1 53 10 34 1 42,2 9 35 1 53 10 87 0,001984876 succinate dehydrogenase [ubiquinone] iron-sulfur 34328286 LOC638710 subunit, mitochondrial precursor 1 31,2 8 18 1 46,8 11 31 1 46,8 11 31 1 46,8 11 80 0,001428569 34536823 Cox6b2 cytochrome c oxidase subunit 6B2 isoform 2 1 47,7 4 5 1 47,7 4 8 1 36,4 3 5 1 47,7 4 19 0,001393207

356582477 Ogdh 2-oxoglutarate dehydrogenase, mitochondrial isoform 1 1 27,6 22 44 0 0 0 0 1 46 39 119 1 54 43 200 0,001669461 358439483 Immt mitochondrial inner membrane protein isoform 2 1 48,5 31 65 1 57 36 171 0 0 0 0 0 0 41 0 - 359385696 Ywhaz 14-3-3 protein zeta/delta isoform 1 1 30,6 8 22 1 42,4 11 31 1 40 9 26 1 49 11 78 0,001972862 359807367 Pkm pyruvate kinase, muscle isoform M1 1 68,9 29 114 1 66,3 30 335 1 67,4 31 363 1 69,3 32 800 0,006761807 enoyl-CoA hydratase domain-containing protein 2, 363543337 Echdc2 mitochondrial isoform 2 1 39,8 5 6 1 39,8 5 6 1 44 6 9 1 44 6 21 0,000902 37574048 Gm6293 cytochrome b-c1 complex subunit 9 1 37,5 2 4 1 37,5 2 4 1 37,5 2 3 1 37,5 2 11 0,001375396 PREDICTED: putative transmembrane protein 377833423 C8orfk29 C8orfK29 1 20,4 4 4 0,9801 6,1 1 1 0,9706 6,1 1 2 1 20,4 4 7 0,00018 38142460 LOC100045699electron transfer flavoprotein subunit beta 1 24,7 5 5 1 15,3 4 4 1 26,7 5 6 1 38,4 8 15 0,000286 pyruvate dehydrogenase phosphatase regulatory 38142488 4930402E16Riksubunit, mitochondrial precursor 1 3,4 3 3 1 6,2 5 5 1 5,5 4 4 1 10 8 12 0,000114

38348482 CCT8L1 chaperonin containing TCP1, subunit 8 (theta)-like 1 1 12,3 6 6 1 17,1 8 8 1 25,4 12 14 1 26,9 13 28 0,000419 384871588 Ace3 angiotensin I converting enzyme 3 precursor 1 17,6 11 21 1 22,5 15 32 1 21 13 34 1 23,9 15 85 0,001051958 38490690 iars2 isoleucine--tRNA ligase, mitochondrial precursor 1 6 5 7 1 19,3 17 26 1 25,2 21 28 1 28,5 24 60 0,000481 38604071 xpo1 exportin-1 1 4 3 3 1 6,3 5 5 1 8,8 8 9 1 11,9 10 16 0,000138 39841061 4922502D21RikC-type lectin-like receptor 2m 1 14,3 3 6 0,9998 10,1 2 4 1 14,3 3 5 1 14,3 3 15 0,000547 39930543 NUP54 nuclear pore complex protein Nup54 1 7,3 3 3 0,9995 5,5 2 2 1 12,4 4 4 1 13,7 5 8 0,000123 40254263 Ubtd2 ubiquitin domain-containing protein 2 1 20,9 4 4 0,9995 14,1 2 2 1 15,8 3 3 1 34,2 6 9 0,000311 40254361 Hspa4l heat shock 70 kDa protein 4L 1 14,8 9 10 1 22,7 14 26 1 36 21 29 1 38,2 23 66 0,000507 40254574 FABP9 fatty acid-binding protein 9 1 53 7 13 1 66,7 9 29 1 66,7 9 19 1 66,7 9 62 0,00166101 NADH dehydrogenase [ubiquinone] iron-sulfur protein 406855423 Ndufs8 8, mitochondrial 1 26,4 5 8 1 29,7 6 11 1 29,7 6 13 1 29,7 6 32 0,000978 407027856 Capzb F-actin-capping protein subunit beta isoform c 1 29,9 6 10 1 49,5 12 25 1 40,9 10 32 1 49,5 13 68 0,001279883

407260963 C1orf101 PREDICTED: uncharacterized protein C1orf101-like 1 7,9 5 5 1 4,5 3 3 1 4,5 3 3 1 7,9 5 10 0,000105 407261678 LOC77908 PREDICTED: uncharacterized protein LOC77908 1 54,8 4 5 1 54,8 4 8 1 54,8 4 10 1 54,8 4 23 0,001835138 407261996 Serpina1 PREDICTED: alpha-1-antiproteinase-like 1 10 3 7 1 10 4 13 1 12,4 5 16 1 12,4 5 35 0,000767 407263255 - PREDICTED: WD repeat-containing protein 66 1 3,3 3 5 1 13,7 11 17 1 11,6 9 14 1 16,3 13 36 0,000225 PREDICTED: leucine-rich repeat-containing protein 407263724 - 37A2 1 9,5 21 57 1 10,5 23 78 1 10,6 22 66 1 11,5 25 203 0,000976

40804746 TEPP testis, prostate and placenta-expressed protein isoform 1 1 34,6 4 4 1 49,5 10 14 1 56,9 12 22 1 54,6 11 37 0,00121632 disintegrin and metalloproteinase domain-containing 440546413 Adam5 protein 5 isoform 1 precursor 1 5,5 4 10 1 4,3 3 6 0,9997 3 2 6 1 6,8 5 23 0,000201 45592928 PPAP2A lipid phosphate phosphohydrolase 1 isoform 1 1 13 3 4 1 13 3 5 0,986 3,5 1 3 1 13 3 11 0,000472 46358405 ATP8B3 probable phospholipid-transporting ATPase IK 1 9,2 9 9 1 18,2 18 20 1 16,3 16 19 1 18,7 19 48 0,000316 46361984 HYDIN hydrocephalus-inducing protein 1 0,8 3 4 1 3,5 16 21 1 4,6 17 24 1 5,6 22 47 0,0000817 46559372 4921511H03Rikuncharacterized protein C7orf62 homolog 1 16,2 4 5 1 17,8 3 4 0,9992 9,1 2 2 1 29,2 7 13 0,000429 46559389 ACE angiotensin-converting enzyme isoform 1 precursor 1 15,4 17 31 0 0 0 0 0 0 0 0 1 18,6 20 97 0,000702 46559428 Gm4763 uncharacterized protein LOC210155 1 14,3 2 9 1 14,3 2 9 1 14,3 2 10 1 14,3 2 28 0,001270718 cytochrome b-c1 complex subunit 1, mitochondrial 46593021 Uqcrc1 precursor 1 49,2 14 57 1 50,6 16 117 1 51,5 17 110 1 50,6 16 281 0,00527534 succinyl-CoA ligase [ADP-forming] subunit beta, 46849708 SUCLA2 mitochondrial precursor 1 58,7 27 189 1 57,2 26 255 1 58,7 28 265 1 58,7 27 714 0,006505705 single-stranded DNA-binding protein, mitochondrial 47059026 SSBP1 isoform 1 1 27,7 3 5 1 43,2 5 6 1 37,8 4 6 1 37,8 4 15 0,000604 471012248 Acr acrosin isoform 3 1 29,7 8 45 1 36,1 9 46 1 27,4 7 42 1 41,3 10 134 0,003268046 47271515 2810408A11Rikprotein phosphatase inhibitor 2-like 1 22,8 7 15 1 27,8 7 13 1 17,2 6 13 1 40,9 11 40 0,000938 476007882 Aldoa1b aldolase 1 A retrogene 2 1 37,9 10 21 1 55,2 17 32 1 51,1 14 28 1 55,5 16 79 0,001391983 cAMP-dependent protein kinase catalytic subunit alpha 483968030 Prkaca isoform 2 1 41,7 13 27 1 41,4 12 29 1 46,6 15 33 1 49 17 95 0,001867731 49355801 Gykl1 glycerol kinase-like 1 1 33,7 14 23 1 38,6 17 67 1 36,2 16 56 1 38,6 17 140 0,002044224 50582595 CCIN calicin 1 32,7 18 32 1 27,2 15 34 1 34,7 18 52 1 40,8 23 118 0,001413515 50582603 Defb22 beta-defensin 22 precursor 1 5,6 2 5 1 10,1 3 17 1 10,1 3 15 1 10,1 3 34 0,001538171 51491845 CLTC clathrin heavy chain 1 1 4,6 6 6 1 2,8 3 3 1 3,4 3 3 1 8 9 11 0,000052

51921341 AA467197 normal mucosa of esophagus-specific gene 1 protein 1 49,4 7 13 1 49,4 6 14 1 49,4 6 14 1 49,4 7 41 0,004605966 54020730 EFR3A protein EFR3 homolog A 1 11,6 7 8 0 0 0 0 0,9746 1,7 1 3 1 11,6 7 11 0,000101 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX succinate dehydrogenase [ubiquinone] flavoprotein 54607098 SDHA subunit, mitochondrial precursor 1 39,5 18 24 1 58,7 26 110 1 61,9 27 111 1 61,9 27 241 0,002725666 55741460 PARK7 protein DJ-1 1 28 5 6 1 40,2 6 10 1 45 7 13 1 51,9 9 32 0,000994 57222276 Adam6b a disintegrin and metalloproteinase domain 6-like 1 9,7 6 11 1 9,7 5 16 1 13,9 8 18 1 18,5 10 56 0,000653 NADH dehydrogenase [ubiquinone] 1 beta subcomplex 58037109 NDUFB10 subunit 10 1 40,9 6 12 1 44,9 6 9 1 44,9 6 10 1 51,7 8 30 0,000887 NADH dehydrogenase [ubiquinone] iron-sulfur protein 58037117 Gm12251 3, mitochondrial precursor 1 34,2 11 31 1 47,1 12 31 1 43,3 11 35 1 47,9 14 96 0,002455362 58037161 LYZL6 lysozyme-like protein 6 precursor 1 18,9 2 3 1 18,9 2 6 0,9996 12,8 1 4 1 18,9 2 13 0,001504172 58037223 Lpcat2b lysophosphatidylcholine acyltransferase 2B 1 7,2 3 3 1 9,3 4 5 0,9997 5,6 2 2 1 9,3 4 10 0,000147 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial 58037235 OXSM precursor 1 12,4 3 5 1 24,6 7 7 1 24 7 7 1 33,6 9 20 0,000361 58037395 SCAMP1 secretory carrier-associated membrane protein 1 1 19,5 6 20 1 27,2 7 37 1 27,2 7 31 1 27,2 7 85 0,003365563 58037413 morn5 MORN repeat-containing protein 5 1 24,7 3 5 1 55,9 6 7 1 57,6 7 13 1 67,1 8 26 0,000939 58037457 Arpm1 actin-related protein T3 1 21,7 5 9 1 21,1 5 6 1 29,8 7 13 1 40,1 9 28 0,000616 59709445 4930579C15Rikequatorin precursor 1 20,2 4 29 1 20,2 4 19 1 20,2 4 27 1 20,2 4 74 0,002197992 59933292 BC049730 uncharacterized protein LOC232972 precursor 1 7,7 2 8 1 14,2 3 11 1 14,2 3 14 1 14,2 3 33 0,001393365 61316404 Odf3b outer dense fiber protein 3B 1 28,2 5 7 1 27,3 5 7 1 21 4 7 1 42 8 23 0,000701 61888838 HSD17B10 3-hydroxyacyl-CoA dehydrogenase type-2 1 41,8 7 12 1 54,4 9 13 1 51 9 12 1 54,8 10 36 0,000791 62000656 Fam71b protein FAM71B 1 18 10 29 1 30,5 15 59 1 28 13 64 1 30,5 15 152 0,002278478 62078759 tekt3 tektin-3 1 24,7 12 13 1 49 23 91 1 46,1 22 92 1 49,2 24 192 0,002396197 65301151 Izumo1 izumo sperm-egg fusion protein 1 precursor 1 25,7 9 51 1 27,7 11 77 1 36 12 68 1 40,1 14 199 0,004170218 66392160 VPS13A vacuolar protein sorting-associated protein 13A 1 1,6 5 5 1 8,1 20 22 1 7,8 20 22 1 10 26 48 0,000148 66392180 Pacrg parkin coregulated gene protein homolog 1 13,7 3 5 0,9998 8,7 2 9 0,9996 8,7 2 7 1 13,7 3 21 0,000588 6671509 ACTB actin, cytoplasmic 1 1 44,3 12 40 1 65,3 16 26 1 68,5 18 42 1 68,5 18 99 0,001476232 6671549 Prdx6 peroxiredoxin-6 1 32,6 6 4 1 57,6 9 8 1 69,6 11 12 1 69,6 11 25 0,000583 6671569 Gm9093 60S acidic ribosomal protein P0 1 10,1 3 3 1 13,9 4 4 0,9993 13,9 2 2 1 23,7 5 9 0,000219 6671678 Car4 carbonic anhydrase 4 precursor 1 33,4 7 13 1 40,3 9 28 1 37,7 8 29 1 40,3 9 66 0,001791858 6671702 cct5 T-complex protein 1 subunit epsilon 1 6,3 3 9 1 18,3 7 13 1 25,1 10 15 1 25,1 10 37 0,000454 6671746 Cfl2 cofilin-2 1 18,7 3 4 1 44,6 5 5 1 44,6 5 6 1 49,4 6 15 0,000442 6678293 Prss39 inactive serine protease 39 precursor 1 21 7 17 1 15,8 6 9 1 21 7 11 1 21 7 36 0,001018452 6678419 tpp2 tripeptidyl-peptidase 2 1 9,7 9 9 1 20,4 18 23 1 18,3 17 19 1 26,6 24 50 0,00031 6678553 vamp3 vesicle-associated membrane protein 3 1 48,5 4 8 1 54,4 4 8 1 55,3 5 9 1 55,3 5 25 0,002123402 6678908 MNS1 meiosis-specific nuclear structural protein 1 1 24,4 12 18 1 17,3 8 12 1 23 10 16 1 25,1 14 45 0,000508 high affinity cAMP-specific and IBMX-insensitive 3',5'- 6679249 PDE8A cyclic phosphodiesterase 8A 1 10 6 6 0,9998 3,9 2 2 1 13,2 8 8 1 14,8 9 17 0,000167 pyruvate dehydrogenase E1 component subunit alpha, 6679261 Pdha1 somatic form, mitochondrial precursor 1 8,2 3 8 1 12,6 5 11 1 22,8 7 12 1 25,1 8 31 0,000475 pyruvate dehydrogenase E1 component subunit alpha, 6679263 pdhA2 testis-specific form, mitochondrial precursor 1 63,4 21 124 1 70,6 22 147 1 70,6 22 165 1 72,9 23 435 0,007200256 6679299 1700071K01Rikprohibitin 1 43 11 33 1 51,1 13 47 1 62,1 14 47 1 65,4 15 126 0,002595415 6679443 PPM1A protein phosphatase 1A 1 12,3 4 5 1 16 5 5 1 15,4 5 8 1 18,1 6 18 0,000315 serine/threonine-protein phosphatase 2B catalytic 6679447 PPP3CC subunit gamma isoform 1 13,3 6 6 1 11,3 5 8 1 17,3 8 17 1 19,1 9 31 0,00052 6679501 psmc1 26S protease regulatory subunit 4 1 9,3 3 3 0,9847 4,8 1 1 1 11,1 4 3 1 15,7 6 7 0,000101 6679583 RAB11B ras-related protein Rab-11B 1 49,1 9 18 1 49,1 9 25 1 52,8 10 29 1 52,8 10 73 0,001802474 6679803 FKBP1A peptidyl-prolyl cis-trans isomerase FKBP1A 1 25 2 3 1 25 2 3 1 25 2 3 1 25 2 9 0,000537 glyceraldehyde-3-phosphate dehydrogenase, testis- 6679939 GAPDHS specific 1 54,3 16 111 1 54,3 16 206 1 62,1 17 189 1 62,1 17 513 0,007178342

6680027 glud1 glutamate dehydrogenase 1, mitochondrial precursor 1 11,6 5 5 1 27,6 12 12 1 25,4 12 17 1 35,1 16 33 0,000365 6680309 Gm2903 10 kDa heat shock protein, mitochondrial 1 40,2 4 5 1 41,2 5 7 1 39,2 5 5 1 49 6 17 0,000562

6680744 Atp1b3 sodium/potassium-transporting ATPase subunit beta-3 1 14,4 3 16 1 27 6 17 1 25,9 5 19 1 29,9 7 50 0,001401258

6680748 Atp5a1 ATP synthase subunit alpha, mitochondrial precursor 1 45,9 19 190 1 48,6 21 278 1 49,4 23 280 1 48,8 22 742 0,007283678 6680806 BPGM bisphosphoglycerate mutase 1 25,9 6 9 1 23,2 5 8 1 26,6 6 9 1 37,1 9 28 0,000756 6680832 Gm7308 calmodulin 1 29,5 3 5 1 34,9 4 8 1 43 5 10 1 43 5 23 0,00093 6681095 Gm10108 cytochrome c, somatic 1 24,8 3 3 1 49,5 5 7 1 48,6 5 7 1 49,5 6 19 0,000902 6752956 ACTL7A actin-like protein 7A 1 41,4 12 18 1 56,6 15 22 1 67 18 31 1 67,3 19 67 0,00130456 6753178 BANF1 barrier-to-autointegration factor 1 40,4 2 4 1 40,4 2 4 1 40,4 2 4 1 40,4 2 12 0,000807 6753320 Cct3 T-complex protein 1 subunit gamma 1 38 19 33 1 30,6 15 44 1 37,8 18 55 1 38 19 132 0,001423076 6753322 cct4 T-complex protein 1 subunit delta 1 18,9 8 18 1 36,9 16 32 1 31,5 14 27 1 37,1 16 74 0,000864 6753324 cct6a T-complex protein 1 subunit zeta 1 19,2 9 14 1 16,4 8 16 1 21,8 11 21 1 21,8 11 50 0,000623 cytochrome c oxidase subunit 4 isoform 1, 6753498 COX4I1 mitochondrial precursor 1 39,1 6 54 1 38,5 5 56 1 39,1 7 48 1 39,1 7 154 0,005429986 6753540 csnk2a2 casein kinase II subunit alpha' 1 54,3 13 31 1 50,6 14 31 1 56,9 14 46 1 59,1 17 106 0,00233044 6753560 Cyct cytochrome c, testis-specific 1 41,9 5 5 1 41,9 5 11 1 41,9 5 12 1 41,9 5 28 0,001382033 6753618 ddt D-dopachrome decarboxylase 1 39 3 4 1 48,3 4 6 1 39 3 4 1 48,3 4 14 0,000634 6754000 Gk2 glycerol kinase 2 1 16,6 7 16 1 33 14 50 1 33,6 14 59 1 36,5 15 128 0,001827615 6754036 Got2 aspartate aminotransferase, mitochondrial 1 14,4 6 11 1 25,3 11 14 1 26,7 10 21 1 29,1 12 45 0,000736 6754086 GSTM5 glutathione S-transferase Mu 5 1 32,6 8 21 1 52,2 12 53 1 48,7 12 57 1 48,7 12 129 0,002420019 6754254 HSP90AA1 heat shock protein HSP 90-alpha 1 13,8 9 10 1 16 11 15 1 24,7 17 27 1 30,6 21 52 0,000506 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 6754660 Cd46 membrane cofactor protein precursor 1 16,7 5 14 1 29 7 17 1 23,6 6 22 1 29 7 55 0,001608731 6754724 psmd7 26S proteasome non-ATPase regulatory subunit 7 1 29,6 6 9 1 27,7 6 8 1 37,4 8 9 1 41,1 9 26 0,000607 6755000 pdcd6 programmed cell death protein 6 1 16,8 3 4 1 17,8 3 3 1 16,8 3 3 1 22,5 4 11 0,000439 6755114 Prdx5 peroxiredoxin-5, mitochondrial precursor 1 17,1 3 5 1 56,7 11 45 1 45,7 10 36 1 56,7 11 86 0,002102354 6755154 CIB1 calcium and integrin-binding protein 1 1 19,9 3 3 0,98 7,3 1 1 0,9998 12 2 2 1 27,2 4 6 0,000182 6755196 Psma4 proteasome subunit alpha type-4 1 23 5 7 1 25,7 5 7 1 36,4 7 11 1 36,4 7 25 0,000673 6755198 Psma6 proteasome subunit alpha type-6 1 24,8 5 7 1 44,3 9 14 1 37,4 7 9 1 48 10 30 0,000728 6755202 PSMB3 proteasome subunit beta type-3 1 28,3 4 5 1 35,1 5 8 1 35,6 5 8 1 35,6 6 20 0,000835 6755204 PSMB5 proteasome subunit beta type-5 1 37,9 9 21 1 49,2 9 13 1 49,2 9 20 1 52,7 11 54 0,001341194 6755210 PSMD13 26S proteasome non-ATPase regulatory subunit 13 1 13 5 7 0,9991 5,9 2 2 1 19,7 6 6 1 26,9 9 15 0,000256 6755372 rps3 40S ribosomal protein S3 1 23,5 5 5 1 12,8 3 3 1 14 3 3 1 28,8 6 11 0,000197 6755614 Spa17 sperm surface protein Sp17 1 40,3 5 11 1 23,5 3 14 1 23,5 3 13 1 40,3 5 39 0,001519277

6755963 VDAC1 voltage-dependent anion-selective channel protein 1 1 21,9 4 4 1 44,2 8 8 1 43,8 9 9 1 46,6 10 20 0,000402

6755965 vdac2 voltage-dependent anion-selective channel protein 2 1 11,9 3 4 1 55,3 13 38 1 52,5 12 30 1 55,3 13 69 0,00134069 67625729 Gm128 protein MENT precursor 1 45,1 10 94 1 56,6 12 85 1 56,9 13 99 1 56,9 13 280 0,009328123 67972647 arsA arylsulfatase A precursor 1 11,9 4 4 1 18,6 6 9 1 20,4 8 11 1 20,4 8 24 0,000711 68226731 npepps puromycin-sensitive aminopeptidase 1 7,3 6 6 1 4,7 4 4 1 16,1 11 11 1 19,1 14 22 0,000185 6996917 G6pdx glucose-6-phosphate 1-dehydrogenase X 1 14,4 7 3 1 29,5 14 14 1 29,7 13 14 1 31,8 14 31 0,000427 70778976 Pgk1-rs7 phosphoglycerate kinase 1 1 17 6 16 1 15,1 5 12 1 17 6 13 1 17 6 39 0,000547 7106277 CSNK2B casein kinase II subunit beta 1 37,7 6 9 1 30,7 5 5 1 33,5 6 6 1 37,7 6 18 0,000791 7106387 psma5 proteasome subunit alpha type-5 1 22,4 4 6 1 30,3 5 7 1 40,2 7 9 1 48,1 8 22 0,000751 7110703 PSMC5 26S protease regulatory subunit 8 1 24,4 8 13 1 22,9 7 7 1 11,1 4 5 1 34,7 11 24 0,000325 71774133 ppiB peptidyl-prolyl cis-trans isomerase B precursor 1 14,8 3 3 1 28,7 6 9 1 49,5 10 11 1 53,7 11 22 0,000558 7242197 psmb1 proteasome subunit beta type-1 precursor 1 20,8 4 4 1 33,8 6 6 1 40,8 7 9 1 40,8 7 19 0,000474 7305229 Ldhc L-lactate dehydrogenase C chain 1 75 20 226 1 81,3 22 419 1 81,9 23 331 1 81,3 22 966 0,011579181 7305241 lrrc23 leucine-rich repeat-containing protein 23 1 13,8 5 8 1 10,6 3 3 1 8,2 3 7 1 18,5 7 21 0,000442 74315965 Hyal5 hyaluronidase PH-20 1 21,7 9 15 1 18,6 8 11 1 28,5 12 18 1 28,7 13 44 0,000837 74315975 PSMD1 26S proteasome non-ATPase regulatory subunit 1 1 9,3 7 10 1 17,4 14 17 1 29 21 28 1 30,2 23 55 0,000469 75677532 1700029J07RikUPF0602 protein C4orf47 homolog 1 12,3 3 6 0,9886 4,4 1 2 0,9612 2,8 1 2 1 17,7 5 11 0,000261 mitochondrial translocator assembly and maintenance 76559944 1500001M20Rikprotein 41 homolog precursor 1 9,8 3 5 1 13,4 3 4 0,9861 3,9 1 2 1 16 4 11 0,000344 7657607 SPAG6 sperm-associated antigen 6 1 21,3 9 40 1 22,9 10 35 1 22,7 10 36 1 22,7 10 109 0,001875728 probable inactive protein kinase-like protein SgK071 76781472 Gm711 homolog 1 5,4 3 3 1 6,9 4 5 1 6,9 4 4 1 6,9 4 12 0,000212 76781476 BC089491 selenoprotein V 1 14 3 5 1 14 3 3 1 14 3 3 1 14 3 11 0,000258 7710014 cul3 cullin-3 1 26,2 17 17 1 29 18 19 1 28,3 18 20 1 36,2 24 57 0,000426 7710018 stom erythrocyte band 7 integral membrane protein 1 32,4 7 22 1 27,1 6 18 1 27,5 6 19 1 35,2 8 58 0,00137285 78214312 Gm12231 ATP synthase subunit b, mitochondrial precursor 1 22,7 8 19 1 27 8 26 1 30,5 10 27 1 30,5 10 72 0,001597778 82904142 Gm1096 PREDICTED: CD177 antigen isoform 2 1 11,2 2 4 1 11,2 2 3 1 11,2 2 7 1 11,2 2 15 0,000511 83715998 Gm2972 ATP synthase subunit e, mitochondrial 1 60,6 4 9 1 60,6 4 5 1 60,6 4 9 1 60,6 4 23 0,001611399 83816907 Gm15785 dnaJ homolog subfamily B member 6 isoform a 1 28,2 9 19 1 24,1 8 10 1 23,8 7 13 1 28,2 9 43 0,001012886 putative ATP-dependent Clp protease proteolytic 8393156 clpP subunit, mitochondrial precursor 1 22,1 5 9 1 20,6 5 7 1 24,6 5 7 1 31,2 7 24 0,0007 serine/threonine-protein phosphatase 2A catalytic 8394024 PPP2CB subunit beta isoform 1 20,1 5 5 1 22 6 7 1 10,7 3 5 1 26,9 7 17 0,000392 84794552 PEBP1 phosphatidylethanolamine-binding protein 1 1 77,5 7 18 1 82,4 9 25 1 88,8 11 38 1 88,8 11 83 0,004091267 84871978 MYCBP C-Myc-binding protein 1 53,4 4 4 0,9998 19,4 1 2 1 65 5 6 1 65 6 12 0,000669 84993736 acsbg2 long-chain-fatty-acid--CoA ligase ACSBG2 1 24,3 12 21 1 22 11 32 1 28,3 12 27 1 27,4 13 79 0,001063661 85662408 Crat carnitine O-acetyltransferase 1 28,4 16 76 1 39,1 21 146 1 35,8 18 140 1 36,9 19 356 0,004584597 85701754 Ccdc114 coiled-coil domain-containing protein 114 1 11,1 5 5 0,9997 3,6 2 6 1 10,2 5 5 1 12,9 6 16 0,000186 85701762 LOC100046792uncharacterized protein C6orf163 homolog 1 20,1 6 12 1 21,3 6 10 1 25 7 9 1 27,4 8 31 0,000526 85701842 Gm216 uncharacterized protein C2orf62 homolog 1 7 3 3 1 8,8 4 4 1 9,6 4 4 1 15,6 7 12 0,000261 85701852 Gm614 uncharacterized protein CXorf65 homolog 1 29,8 5 8 0,9999 13,6 2 5 1 21,5 4 6 1 33,5 6 19 0,000573 85701985 Gm1060 dynein regulatory complex protein 1 1 9 7 10 1 11,7 8 11 1 9,2 6 10 1 15,3 12 32 0,000309 85702063 1700071K01Rikprohibitin-like 1 41 9 8 1 38,7 10 7 1 55 11 13 1 58,3 12 29 0,000635 87239970 lipe hormone-sensitive lipase isoform 1 1 19 11 12 1 5,9 3 5 1 12 7 12 1 21,2 12 29 0,000325 88196767 MLF1 myeloid leukemia factor 1 isoform a 1 20,2 5 10 1 25,1 4 7 0 0 0 0 0 0 7 0 - 89886473 9030607L17Rikizumo sperm-egg fusion protein 4 precursor 1 31,3 7 38 1 34,4 8 42 1 34,4 8 39 1 34,4 8 118 0,003400782 phospholipid hydroperoxide glutathione peroxidase, 90903233 Gpx4 nuclear isoform 1 1 16,8 3 6 1 48,7 10 47 1 48,2 9 37 1 48,7 10 85 0,001711689 9256624 PGAM2 phosphoglycerate mutase 2 1 45,5 12 49 1 57,3 16 67 1 66,4 15 61 1 71,5 17 171 0,00470782 93102411 GALC galactocerebrosidase precursor 1 8 5 5 1 5 3 4 0,9996 3,9 2 2 1 9,1 6 11 0,000198 94536844 rpiA ribose-5-phosphate isomerase 1 19,1 5 7 0,9997 8,9 2 3 0,9995 5,3 2 3 1 19,1 5 12 0,000253 vesicle-associated membrane protein-associated protein 94721328 vapa A 1 17,3 4 6 1 11,6 3 7 1 10,4 2 5 1 17,3 4 18 0,000627 9506739 nagK N-acetyl-D-glucosamine kinase isoform 1 1 35,3 9 14 1 36,2 8 13 1 36,4 8 12 1 44,6 10 39 0,00098 95772156 1700019N12Riktestis-expressed sequence 40 protein 1 17,5 3 6 0,9997 11,9 2 2 1 16,5 3 4 1 21,6 4 12 0,000547 LETM1 and EF-hand domain-containing protein 1, 9789997 LETM1 mitochondrial precursor 1 14,9 7 8 1 27,4 13 19 1 32,9 18 24 1 31,3 18 49 0,000464 9790051 pfkp 6-phosphofructokinase type C 1 30,9 18 31 1 46,9 29 77 1 44,9 27 70 1 46 30 167 0,001817018 9790055 Gm2199 mitochondrial carrier homolog 2 1 15,8 4 6 1 38,9 9 21 1 29,7 7 12 1 38,9 9 39 0,001167426 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 9790131 Slc22a21 solute carrier family 22 member 21 1 7,4 3 5 1 6,7 3 5 1 8,9 4 8 1 11 5 18 0,000458 9910582 Tex101 testis-expressed sequence 101 protein precursor 1 26,8 5 7 1 30,4 6 14 1 30,4 6 17 1 30,4 6 38 0,001396415 NAD kinase domain-containing protein 1, 146134392 1110020G09Rikmitochondrial isoform 1 0,9999 8,4 3 3 0,9846 3,3 1 1 0,9677 3,3 1 1 1 11,6 4 7 0,0000979 168229152 Gm13570 brain protein 44-like protein 2 0,9999 26,1 3 12 1 45,4 4 12 1 45,4 4 11 1 45,4 4 37 0,002277964 18859597 LOC675851 NADH dehydrogenase [ubiquinone] 1 subunit C2 0,9999 32 3 6 1 14,4 2 4 1 33 4 7 1 40,2 5 18 0,001116575 21746161 Tubb2a-ps2 tubulin beta-2B chain 0,9999 54,6 19 10 0,9838 51,9 19 5 1 61,8 20 12 1 65,2 21 27 0,000313 dolichyl-diphosphooligosaccharide--protein 282398108 RPN1 glycosyltransferase subunit 1 precursor 0,9999 4,9 3 3 1 6,1 3 3 1 15,1 7 8 1 18,1 9 14 0,00015 6678465 Tuba3a tubulin alpha-3 chain 0,9999 53,6 19 71 1 64,9 22 70 0,9996 64,2 23 71 0,9998 74,9 24 197 0,002186908 9789907 STUB1 STIP1 homology and U box-containing protein 1 0,9999 10,9 3 3 0,9988 6,2 2 2 0,999 7,2 2 2 1 13,8 4 7 0,000121 110625843 LRRC34 leucine-rich repeat-containing protein 34 0,9998 6 2 3 1 15,9 6 6 1 15,7 6 6 1 30,6 11 16 0,000304 110626034 4930519F16Rikuncharacterized protein LOC75106 precursor 0,9998 9,1 2 5 1 30,7 4 11 1 30,7 4 16 1 30,7 4 31 0,001443312 12963687 SSNA1 Sjoegren syndrome nuclear autoantigen 1 homolog 0,9998 20,2 2 2 1 33,6 3 3 0,9996 20,2 2 2 1 34,5 4 9 0,000435 glycerophosphodiester phosphodiesterase domain- 188219547 Gdpd4 containing protein 4 0,9998 3,8 2 6 0,9998 3,8 2 2 0,9998 3,8 2 5 1 5,5 3 14 0,000306 20799907 HIST2H3B histone H2A type 2-A 0,9998 21,9 2 3 1 20,3 2 4 0,9994 14,8 1 2 0,9998 21,9 2 8 0,000478 213688406 Gm11582 dynein light chain 1, cytoplasmic 0,9998 49,4 3 5 1 50,6 4 9 0,9999 58,4 4 5 1 58,4 4 19 0,00162785 PREDICTED: up-regulated during skeletal muscle 309265053 - growth protein 5-like 0,9998 43,1 2 2 0,9999 43,1 2 4 0,9863 25,9 1 3 0,9999 43,1 2 9 0,001270842 318043086 LOC73863 uncharacterized protein LOC73863 0,9998 25,2 2 2 0,9999 25,2 2 2 0,9999 25,2 2 2 0,9999 25,2 2 6 0,00034

33469095 Pcsk4 proprotein convertase subtilisin/kexin type 4 precursor 0,9998 3,4 2 2 0 0 0 0 0,9822 2,1 1 2 0,9998 3,4 2 4 0,0000701 58037257 dcaf7 DDB1- and CUL4-associated factor 7 0,9998 8,2 2 2 0,9892 4,4 1 1 0,9996 8,2 2 2 0,9999 8,2 2 5 0,000122 PREDICTED: mitochondrial pyruvate carrier-like 94366539 Gm13570 protein-like 0,9998 42,6 4 10 1 61,5 5 9 0,997 44,3 4 7 1 61,5 5 28 0,001715179 113931146 1700019L03Rikuncharacterized protein C9orf117 homolog 0,9997 4,6 2 4 0,9896 2,9 1 3 0,9596 2,9 1 1 0,9997 4,6 2 8 0,000107 125988403 SPCS3 signal peptidase complex subunit 3 0,9997 11,7 2 2 1 17,8 3 4 0,9997 11,1 2 2 1 17,8 3 9 0,000394 194353962 Gm8230 ADP-ribosylation factor 1 0,9997 19,3 3 3 1 53,6 7 9 0,973 53,6 7 1 0,9777 53,6 7 4 - 21314844 CATSPER1 cation channel sperm-associated protein 1 0,9997 1,9 1 2 0,9999 1,9 1 2 0,9815 1,9 1 1 0,9998 1,9 1 5 0,00013 228008337 psmc3 26S protease regulatory subunit 6A 0,9997 5,7 2 3 0,9999 5,7 2 3 1 8,4 3 4 1 8,4 3 10 0,000135 electron transfer flavoprotein-ubiquinone 254588014 Etfdh oxidoreductase, mitochondrial precursor 0,9997 3,7 2 2 1 7 4 4 1 16,1 8 8 1 17,4 9 14 0,000165 255760052 COPS7A COP9 signalosome complex subunit 7a isoform 2 0,9997 7,6 2 2 0,9999 9,5 2 2 0,9998 9,5 2 2 1 12,7 3 6 0,000161 257467659 Dnahc6 axonemal dynein heavy chain 0,9997 0,6 2 2 1 10,5 37 47 1 8,5 31 37 1 12,4 43 84 0,000147 28893247 cyb5r2 NADH-cytochrome b5 reductase 2 isoform 2 0,9997 7,2 2 2 1 12,7 3 3 1 17 4 4 1 17 4 9 0,000255 29244338 4933400C05Rikspermatogenesis-associated protein 32 0,9997 9 2 2 1 24,9 6 10 1 31,4 8 19 1 31,4 8 31 0,000815 294460010 LOC69439 uncharacterized protein LOC69439 0,9997 2 2 2 1 5,8 5 8 1 4,8 4 4 1 7,4 6 14 0,000124 306966148 Smpd5 sphingomyelin phosphodiesterase 5 0,9997 6 2 2 1 6 2 5 1 6 2 5 1 6 2 10 0,00022 31981468 IMMP2L mitochondrial inner membrane protease subunit 2 0,9997 11,4 2 3 1 26,9 3 4 1 31,4 4 5 1 31,4 4 12 0,000422 41056093 Nif3l1 NIF3-like protein 1 0,9997 6,4 2 3 1 14,4 3 3 1 18,1 4 6 1 21 5 12 0,000282 51556221 Ces7 carboxylesterase 5A precursor 0,9997 2,4 1 2 1 12,3 5 7 1 13,7 6 20 1 13,7 6 28 0,000678 58037149 2200002J24Rikuncharacterized protein LOC69147 0,9997 21,8 2 2 0,9793 8,2 1 1 0,9704 8,2 1 2 1 21,8 2 6 0,000433 7305185 INSL6 insulin-like peptide INSL6 precursor 0,9997 8,9 2 2 0,9997 8,9 2 2 0,9994 8,9 2 2 0,9997 8,9 2 6 0,000234 serine/threonine-protein phosphatase 2A 65 kDa 8394027 PPP2R1A regulatory subunit A alpha isoform 0,9997 3,4 2 2 1 5,8 3 3 1 13,8 6 6 1 17,7 8 11 0,000132 91206392 MPI mannose-6-phosphate isomerase 0,9997 7,6 2 3 1 20,3 7 8 1 42,1 11 15 1 45,6 12 25 0,000529 NADH dehydrogenase [ubiquinone] 1 alpha 9506911 NDUFA1 subcomplex subunit 1 0,9997 14,3 1 3 0,9998 14,3 1 2 0,9997 14,3 1 2 0,9998 14,3 1 7 0,000595 10181184 ATP5J2 ATP synthase subunit f, mitochondrial 0,9996 26,1 2 5 1 34,1 3 8 0,9998 26,1 2 7 1 34,1 3 19 0,001238591 110625928 PRSS44 serine protease 44 precursor 0,9996 6,7 2 3 0,9879 3 1 1 0,9837 3 1 1 0,9997 6,7 2 5 0,000161 118150478 4930578N16Rikcoiled-coil domain-containing protein 173 0,9996 3,5 2 2 0,9774 1,6 1 1 0,9659 1,8 1 1 1 5,3 3 4 0,0000412 120300928 KCNU1 potassium channel subfamily U member 1 0,9996 1,8 2 2 1 2,8 3 3 1 6,2 6 7 1 6,2 6 12 0,000133 121674797 PPT1 palmitoyl-protein thioesterase 1 precursor 0,9996 10,8 2 3 1 13,7 3 3 1 18 4 7 1 20,9 5 14 0,000409 13384870 Tmem126a transmembrane protein 126A 0,9996 8,7 2 4 0,9892 4,6 1 2 0,9991 8,7 2 2 0,9997 8,7 2 8 0,000426 145966883 PSMD14 26S proteasome non-ATPase regulatory subunit 14 0,9996 6,5 2 2 0,9997 6,5 2 2 0,9996 6,5 2 2 0,9997 6,5 2 6 0,000195 158631221 1700093K21Rikuncharacterized protein C2orf74 homolog 0,9996 12,2 2 5 1 18,4 3 3 0,9997 12,2 2 5 1 18,4 3 14 0,000604 160333353 trim69 E3 ubiquitin-protein ligase TRIM69 0,9996 4,2 2 2 1 29,8 12 21 1 28,8 12 15 1 33,4 14 37 0,0005 160415213 RHOC rho-related GTP-binding protein RhoC precursor 0,9996 14,5 2 2 1 19,2 3 4 0 0 0 0 0 0 3 0 - 19482172 rdh11 retinol dehydrogenase 11 precursor 0,9996 7 2 2 0,986 4,4 1 1 1 11,1 3 3 1 11,1 3 6 0,000159 19526900 TMEM30A cell cycle control protein 50A 0,9996 6,9 2 3 0,9897 4,7 1 1 1 14,8 3 3 1 17 4 7 0,000205 19745150 cyb5r3 NADH-cytochrome b5 reductase 3 0,9996 7 2 2 1 13,6 4 4 1 21,3 5 5 1 21,3 5 11 0,000255 dnaJ homolog subfamily A member 3, mitochondrial 205361112 DNAJA3 isoform 1 0,9996 5,3 2 4 0,9996 4,2 2 2 0,9998 6,2 2 2 1 10,4 4 8 0,00011 205361116 Mdh1b putative malate dehydrogenase 1B 0,9996 4,6 2 2 0,9996 3,4 2 2 0,9737 1,8 1 2 1 8,2 4 6 0,000105 21313468 TSFM elongation factor Ts, mitochondrial precursor 0,9996 13,6 2 2 0,9998 13,6 2 3 0,9994 13,6 2 2 0,9997 13,6 2 7 0,000192 226874906 YWHAE 14-3-3 protein epsilon 0,9996 12,5 3 2 1 20,4 5 3 1 22 5 4 1 27,1 6 9 0,00019 cytochrome c oxidase subunit 7A-related protein, 226958512 Cox7a2l mitochondrial isoform 1 0,9996 40,5 2 3 0,9998 40,5 2 2 0,9995 40,5 2 2 0,9997 40,5 2 7 0,000483 247270151 ftmt ferritin, mitochondrial precursor 0,9996 9,7 2 3 1 32,9 5 13 1 28,7 4 6 1 32,9 5 21 0,000613

254675162 NUP210L nuclear pore membrane glycoprotein 210-like precursor 0,9996 1,4 2 2 1 7,5 12 13 1 3,2 4 4 1 9,4 14 19 0,0000865 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX PREDICTED: mitochondrial import inner membrane 309268906 Tim14 translocase subunit TIM14-like 0,9996 19,8 2 2 0,9552 7,8 1 1 0 0 0 0 0,9997 19,8 2 3 0,000134 31541988 CCDC38 coiled-coil domain-containing protein 38 0,9996 4,3 2 2 0 0 0 0 0 0 0 0 0,9997 4,3 2 2 0,000022 31560116 1110032A03RikUPF0686 protein C11orf1 homolog 0,9996 12,7 2 3 0,9995 12,7 2 4 0,9996 12,7 2 5 0,9997 12,7 2 11 0,000659 45597447 Gm8566 superoxide dismutase [Cu-Zn] 0,9996 16,2 2 4 1 26,6 4 6 1 31,2 4 9 1 35,7 5 19 0,000771 51591899 PPP3R2 calcineurin subunit B type 2 0,9996 12,8 2 2 0,9999 12,8 2 3 1 19 3 7 1 19 3 12 0,000412 58037499 LCN12 epididymal-specific lipocalin-12 precursor 0,9996 5,7 1 4 1 12,4 2 3 0,9997 5,7 1 5 1 12,4 2 12 0,000809 6679066 NIPSNAP1 protein NipSnap homolog 1 0,9996 7,7 2 2 0 0 0 0 0,9995 15,5 2 2 1 18,3 3 4 0,000101 6753520 CREG1 protein CREG1 precursor 0,9996 13,2 2 4 1 16,4 3 11 1 21,8 4 9 1 21,8 4 24 0,000926 6755560 SLC4A1 band 3 anion transport protein 0,9996 2,5 2 2 1 4,4 4 4 1 5,8 5 7 1 5,8 5 13 0,000201 67846103 SHMT1 serine hydroxymethyltransferase, cytosolic 0,9996 4,4 2 3 1 12,3 5 5 0,9996 4,6 2 2 1 12,3 5 10 0,000151 85662421 ACTL9 actin-like protein 9 0,9996 7,2 2 3 1 26 6 7 1 21 5 6 1 33,5 7 14 0,000364

9789985 ivd isovaleryl-CoA dehydrogenase, mitochondrial precursor 0,9996 5,4 2 4 1 8,3 3 3 1 8,3 3 3 1 10,4 4 11 0,000184 110626066 MORN3 MORN repeat-containing protein 3 0,9995 9,1 2 2 1 29,9 6 6 1 38,2 7 8 1 38,2 7 16 0,000409 124486704 4933401F05Rikserine protease 55 precursor 0,9995 9,7 2 2 0 0 0 0 0,9804 3,4 1 1 0,9996 9,7 2 3 0,000169 125991758 SPEF2 sperm flagellar protein 2 0,9995 2,6 2 2 0 0 0 0 0,9863 1,5 1 1 0,9998 2,6 2 3 0,0000262 126116585 KRT1 keratin, type II cytoskeletal 1 0,9995 3,6 2 11 1 3,8 3 16 0,9995 3,6 2 10 1 3,8 3 37 0,000683 13384756 EEF1E1 eukaryotic translation elongation factor 1 epsilon-1 0,9995 9,8 2 3 0,9876 5,7 1 1 1 14,9 3 4 1 14,9 3 8 0,000295 13385186 CCDC96 coiled-coil domain-containing protein 96 0,9995 4,8 2 4 0,9993 4,8 2 2 0,9995 4,8 2 4 0,9997 4,8 2 10 0,000121 13385518 Arl8b ADP-ribosylation factor-like protein 8B 0,9995 8,6 2 2 1 13,4 3 3 1 28,5 4 4 1 33,3 5 9 0,000287

165932339 cutC copper homeostasis protein cutC homolog isoform 2 0,9995 12,6 2 4 1 32,8 5 9 1 41,6 7 11 1 41,6 7 23 0,000555 17505218 GHITM growth hormone-inducible transmembrane protein 0,9995 6,4 2 2 0,9998 6,4 2 3 1 6,4 2 3 1 6,4 2 7 0,000237 18087731 Dynll2 dynein light chain 2, cytoplasmic 0,9995 49,4 3 4 1 50,6 4 9 1 49,4 3 5 1 49,4 3 17 0,001210594 21311849 Ggct gamma-glutamylcyclotransferase 0,9995 12,8 2 2 0,9781 6,9 1 2 1 17,6 3 3 1 17,6 3 7 0,000207 223468646 SH3GL3 endophilin-A3 isoform 1 0,9995 12,1 2 2 1 19,3 5 5 1 15 4 5 1 28,5 8 12 0,000232 251823978 Pcx pyruvate carboxylase, mitochondrial isoform 2 0,9995 2 2 2 0 0 0 0 1 3,2 3 3 1 4,1 4 5 0,0000306 spermidine/spermine N(1)-acetyltransferase-like protein 254587975 SATL1 1 0,9995 3,6 2 2 1 6,9 3 3 1 5 3 6 1 8,2 4 11 0,000185 mitochondrial import inner membrane translocase 254675168 Gm10171 subunit Tim23 0,9995 12,9 2 3 0,9899 8,1 1 1 0,9861 8,1 1 1 0,9996 12,9 2 5 0,000264 NFU1 iron-sulfur cluster scaffold homolog, 282154801 Gm7859 mitochondrial isoform 2 precursor 0,9995 10,6 2 2 0,9997 11 2 2 0,9811 4,7 1 1 1 18,8 4 6 0,000222 31980691 DKKL1 dickkopf-like protein 1 precursor 0,9995 9,6 2 5 1 13 3 3 0,9994 7,4 2 5 1 13 3 13 0,000349 membrane-associated progesterone receptor component 31980806 PGRMC1 1 0,9995 14,4 2 2 0 0 0 0 0,98 4,1 1 2 0,9996 14,4 2 4 0,000136 31981562 Gm6560 pyruvate kinase, muscle isoform M2 0,9995 66,1 27 3 1 63,5 28 6 0,9996 64,6 29 5 1 66,5 30 14 0,000117 mitochondrial import inner membrane translocase 33468937 TIMM17B subunit Tim17-B 0,9995 12,8 2 2 0,9993 12,8 2 2 1 21,5 3 4 1 21,5 3 7 0,000332 54261793 atp2b4 plasma membrane calcium ATPase 4 isoform b 0,9995 25,1 23 2 0,9997 25,7 24 2 1 25,7 24 50 1 25,7 24 142 0,001207879 6755901 Gm7172 tubulin alpha-1A chain 0,9995 54,8 19 25 0,9997 55,4 21 24 0,9997 65,4 23 24 0,9997 65,4 23 77 0,000876 76253942 cetn1 centrin-1 0,9995 10,5 2 3 0,9999 17,4 2 3 1 22,7 3 3 1 27,9 4 9 0,000327 77404227 1700024G13RikUPF0728 protein C10orf53 homolog 0,9995 24,7 2 2 0,9841 9,7 1 1 0,9996 20,4 2 2 1 35,5 3 5 0,000347 ATP synthase-coupling factor 6, mitochondrial 7949005 LOC674583 precursor 0,9995 26,9 2 2 0,9985 17,6 2 2 0 0 0 0 1 35,2 3 3 0,000138 20911031 KRT5 keratin, type II cytoskeletal 5 0,9994 7,1 4 6 0,9999 7,4 4 3 0,974 3,8 2 5 0,9998 10,3 6 4 0,000073 22164770 Oit1 protein FAM3D precursor 0,9994 9,4 2 2 1 13 3 3 1 17,5 4 6 1 17,5 4 11 0,000321 225543134 tk2 thymidine kinase 2, mitochondrial precursor 0,9994 6,7 2 2 0,9811 3,3 1 1 0,9856 3,3 1 1 0,9997 6,7 2 4 0,000109 227430395 Lrrc57 leucine-rich repeat-containing protein 57 isoform b 0,9994 9,2 2 2 1 23,4 5 8 1 26,4 6 10 1 26,4 6 21 0,00059 hydroxyacylglutathione hydrolase, mitochondrial 227499238 HAGH isoform 1 precursor 0,9994 4,6 1 2 1 19,7 5 8 1 20 4 5 1 24,9 6 14 0,000354 241666436 Ribc2 RIB43A-like with coiled-coils protein 2 0,9994 5,3 2 2 1 32,6 10 11 1 26,3 9 14 1 35,8 11 25 0,000424

242247071 EFCAB10 EF-hand calcium-binding domain-containing protein 10 0,9994 12,1 2 2 0,9996 13,6 2 2 0,9988 12,1 2 2 1 18,9 3 6 0,000373 leucine-rich repeat and guanylate kinase domain- 254588006 LRGUK containing protein 0,9994 2,4 2 2 0 0 0 0 0,9621 1,1 1 1 0,9995 2,4 2 4 0,0000439 254588114 Ttc39d tetratricopeptide repeat domain 39D 0,9994 5,1 2 3 1 23,3 10 15 1 17,1 7 11 1 23,3 10 30 0,000426 261245029 CCDC146 coiled-coil domain containing 146 0,9994 2,1 2 2 0 0 0 0 0,9816 1,1 1 1 1 3 3 4 0,0000268 282721029 CTBP2 C-terminal-binding protein 2 isoform 1 0,9994 4,3 2 4 1 13 4 4 1 8,5 3 3 1 14,8 5 11 0,00011 294610650 Gstt4 glutathione S-transferase theta-4 0,9994 12,1 2 2 0,9995 10 2 4 1 12,9 3 6 1 18,8 4 12 0,000328 58037359 1700011F03Riktestis-expressed sequence 37 protein 0,9994 10,6 2 3 1 40 5 9 1 55,6 6 12 1 60 7 24 0,001006744 61656190 Lrrc52 leucine-rich repeat-containing protein 52 precursor 0,9994 6,7 2 3 0,9997 6,7 2 2 0,9861 4,5 1 3 0,9996 6,7 2 8 0,000318 6755368 LOC10004732940S ribosomal protein S18 0,9994 13,2 2 2 0,9719 5,7 1 1 0 0 0 0 1 18,4 3 4 0,000139 6755688 STXBP2 syntaxin-binding protein 2 0,9994 4,2 2 2 0,9833 1,7 1 2 1 7,8 3 3 1 10,3 4 7 0,0000792 7305619 USP5 ubiquitin carboxyl-terminal hydrolase 5 0,9994 3,1 2 2 0,9879 1,4 1 1 0,9997 3,1 2 2 1 6,3 4 5 0,0000453 dihydroorotate dehydrogenase (quinone), mitochondrial 9910194 dhodh precursor 0,9994 4,8 2 2 0 0 0 0 0,9912 3,5 1 1 1 8,4 3 3 0,000058 110625851 Tmprss12 transmembrane protease serine 12 precursor 0,9993 7,1 2 2 0 0 0 0 0,9994 7,1 2 2 0,9995 7,1 2 4 0,000113 160298209 GOT1 aspartate aminotransferase, cytoplasmic 0,9993 6,5 2 3 1 13,8 5 5 1 19,4 7 7 1 22,5 8 15 0,000213

166851828 Atp5d ATP synthase subunit delta, mitochondrial precursor 0,9993 13,7 2 3 1 13,7 2 5 0,9994 13,7 2 3 1 13,7 2 10 0,00064 16716569 PRSS1 protease, serine, 1 precursor 0,9993 8,1 1 10 0,9996 8,1 1 9 0,9995 8,1 1 13 0,9996 8,1 1 33 0,001755553 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX

21312758 PPP6C serine/threonine-protein phosphatase 6 catalytic subunit 0,9993 8,2 2 2 0,9998 8,9 2 2 0,9996 9,2 2 2 1 13,1 3 6 0,000124 21919408 ODF4 outer dense fiber protein 4 0,9993 7,2 2 2 0 0 0 0 0 0 0 0 0,9995 7,2 2 2 0,0000902 222831630 IQCF5 IQ domain-containing protein F5 0,9993 8,1 1 2 0,989 8,1 1 1 0,9828 8,1 1 1 0,9998 8,1 1 4 0,000151 NADH dehydrogenase [ubiquinone] 1 alpha 226437665 NDUFA11 subcomplex subunit 11 0,9993 23,8 2 2 0,9996 23,8 2 2 1 35 3 5 1 35 3 10 0,000654 227116292 DPP4 dipeptidyl peptidase 4 isoform 2 0,9993 3,3 2 2 1 6 4 4 1 11 7 7 1 11 7 13 0,000142 electron transfer flavoprotein subunit alpha, 227500281 etfA mitochondrial 0,9993 7,8 2 2 0,9996 7,5 2 2 1 20,1 4 4 1 27,6 6 8 0,000151 255683447 LOC100046727IQ and ubiquitin-like domain-containing protein 0,9993 2,7 2 2 0,9996 3,4 2 3 1 7,9 5 6 1 11,2 7 11 0,000105 31560474 dgkA diacylglycerol kinase alpha 0,9993 2,2 2 2 0 0 0 0 0 0 0 0 0,9995 2,2 2 2 0,0000199 85701692 1700057G04Rikuncharacterized protein LOC78459 0,9993 7,3 2 2 0 0 0 0 0 0 0 0 0,9995 7,3 2 2 0,000119 9790191 Gm9840 E3 ubiquitin-protein ligase RBX1 0,9993 24,1 2 2 0,9997 24,1 2 2 0,986 16,7 1 1 0,9996 24,1 2 5 0,000475 13385654 4921536K21Rikuncharacterized protein C5orf52 homolog 0,9992 8,3 2 2 0,9784 7,7 1 2 1 33,3 4 5 1 33,9 5 9 0,000353 vesicle transport through interaction with t-SNAREs 13928668 vti1a homolog 1A 0,9992 8,8 2 2 0 0 0 0 0 0 0 0 0,9994 8,8 2 2 0,0000676 156713423 PSMD8 26S proteasome non-ATPase regulatory subunit 8 0,9992 5,1 2 2 0,9993 4,5 2 2 1 17,8 4 4 1 20,7 5 9 0,000211 G-protein coupled receptor family C group 5 member B 23956118 Gprc5b isoform 2 precursor 0,9992 5,9 2 3 0,9813 2,9 1 1 0,9987 5,9 2 2 0,9994 5,9 2 7 0,000372 254587916 1700094D03Rikuncharacterized protein C1orf189 homolog 0,9992 21,7 2 2 0,9619 8,5 1 1 0,9994 21,7 2 2 0,9994 21,7 2 5 0,000278 7106335 KRT17 keratin, type I cytoskeletal 17 0,9992 8,3 4 7 0,9998 12,5 6 3 0 0 0 0 0,9998 12,5 6 6 0,000111 91206446 Gm14316 serine protease inhibitor, Kunitz type 5 precursor 0,9992 20 2 2 0,9785 11,4 1 1 0,9861 11,4 1 1 1 28,6 3 5 0,00034 119392079 LOC100044248uncharacterized protein C12orf60 homolog 0,9991 10,5 2 2 1 22,3 5 6 1 22,3 5 6 1 22,3 5 14 0,000338 13277394 GRPEL1 grpE protein homolog 1, mitochondrial precursor 0,9991 8,8 2 2 0,9995 8,8 2 2 1 13,8 3 3 1 13,8 3 7 0,000199 146260278 GPX6 glutathione peroxidase 6 precursor 0,9991 5,4 1 2 0,9992 5,4 1 2 0,9997 5,4 1 2 0,9998 5,4 1 6 0,000277 16716349 Nup62 nuclear pore glycoprotein p62 0,9991 4,6 2 2 0,9845 2,7 1 1 0 0 0 0 0,9995 4,6 2 3 0,000103 carcinoembryonic antigen-related cell adhesion 229092064 Ceacam10 molecule 10 precursor 0,9991 6,8 2 2 0,9996 6,8 2 2 0,9994 6,8 2 2 1 11,3 3 7 0,00033 239985513 atad3a ATPase family AAA domain-containing protein 3 0,9991 4,9 2 6 0,9987 4,9 2 2 0 0 0 0 0,9993 4,9 2 7 0,0000767 285401645 1700001C19Rikuncharacterized protein LOC75462 isoform 2 0,9991 21,7 2 2 0,9994 21,7 2 2 0,9996 18,3 2 2 1 32,2 3 6 0,000299 PREDICTED: NADH dehydrogenase [ubiquinone] iron- 309265963 Ndufs6 sulfur protein 6, mitochondrial-like 0,9991 31,9 2 2 1 51,7 4 5 1 41,4 3 3 1 51,7 4 10 0,000451 33468857 HINT1 histidine triad nucleotide-binding protein 1 0,9991 27 2 2 1 27 2 3 1 36,5 3 4 1 71,4 5 10 0,000569 58037325 Dusp21 dual specificity phosphatase 21 0,9991 13,8 2 3 1 13,8 2 5 1 13,8 2 3 1 13,8 2 11 0,000974 66841374 1700007G11Rikuncharacterized protein C4orf22 homolog 0,9991 6,9 2 3 0,9744 3,4 1 2 0,9989 11,2 2 5 0,9994 6,9 2 9 0,000332 6755120 38231 septin-4 0,9991 3,6 2 4 1 15,7 6 22 1 18 7 21 1 18 7 46 0,000723 72534843 SVOP synaptic vesicle 2-related protein 0,9991 4,6 2 2 0 0 0 0 0 0 0 0 0,9994 4,6 2 2 0,0000446 168229259 MYCBPAP MYCBP-associated protein 0,999 2,1 2 2 0 0 0 0 0 0 0 0 0,9993 2,1 2 2 0,0000232 23943838 slc25a1 solute carrier family 25, member 1 0,999 6,4 2 2 1 12,9 4 4 1 12,9 4 4 1 12,9 4 10 0,000238 7710086 RAB10 ras-related protein Rab-10 0,999 11,5 2 2 0 0 0 0 0,9993 11,5 2 2 0,9996 11,5 2 4 0,000107 uncharacterized protein C2orf47 homolog, 145587094 9430016H08Rikmitochondrial precursor 0,9989 6,9 2 2 1 21 6 6 1 17,9 5 5 1 21 6 14 0,000324 23956058 PLP1 myelin proteolipid protein 0,9989 8,7 2 2 0 0 0 0 0,9993 6,9 2 3 1 11,6 3 6 0,000256 34304087 SPINK2 serine protease inhibitor Kazal-type 2 precursor 0,9989 16,3 1 2 0,9998 16,3 1 2 0,9715 16,3 1 1 0,9997 16,3 1 5 0,000401 377834422 Prdx1 PREDICTED: peroxiredoxin-1 0,9989 8 2 2 0 0 0 0 0 0 0 0 0 0 4 0 - calcium-binding mitochondrial carrier protein SCaMC- 49274632 SLC25A23 3 0,9989 4,5 2 2 0 0 0 0 0 0 0 0 0,9992 4,5 2 3 0,0000429 6755536 slc16a7 monocarboxylate transporter 2 0,9989 3,9 2 4 0,9995 3,9 2 4 0,9992 3,9 2 3 0,9994 3,9 2 11 0,000538 9789881 Arl3 ADP-ribosylation factor-like protein 3 0,9989 10,4 2 2 1 23,6 3 3 1 28 4 6 1 28 4 12 0,000401 244790071 Lrrc51 leucine-rich repeat-containing protein 51 isoform 1 0,9988 10,4 2 2 1 26,6 4 5 1 21,9 3 7 1 26,6 4 14 0,000585 247269607 Dguok deoxyguanosine kinase, mitochondrial isoform 1 0,9988 7,9 2 2 0,9995 9,7 2 2 1 20,6 4 4 1 20,6 4 8 0,000218 254553418 1700019O17Rikuncharacterized protein C2orf57 homolog 0,9988 5,1 2 2 0,9891 3,4 1 4 1 7,4 3 4 1 7,4 3 10 0,000313 28916703 Prdx6-rs1 peroxiredoxin 6, related sequence 1 0,9988 23,2 4 2 1 49,1 8 9 1 44,6 7 8 1 57,1 9 18 0,000433 41235733 LYRM4 LYR motif-containing protein 4 0,9988 22 2 2 0,9992 19,8 2 2 0,999 19,8 2 2 1 33 3 6 0,000396 9910550 SEC11A signal peptidase complex catalytic subunit SEC11A 0,9988 9,5 2 2 1 15,6 3 4 1 15,6 3 3 1 15,6 3 9 0,000309 407261456 Lipe PREDICTED: hormone-sensitive lipase-like 0,9987 11,1 2 2 1 14,7 3 3 0,9995 9 2 2 1 14,7 3 7 0,000239 198278455 1700029J11Rikuncharacterized protein LOC76426 precursor 0,9986 14,1 2 2 0 0 0 0 0,9993 14,1 2 2 0,9996 14,1 2 4 0,000221 268370090 WDR64 WD repeat-containing protein 64 0,9986 2,2 2 3 1 7,4 7 11 1 10,9 10 11 1 13,1 12 25 0,000175 NADH dehydrogenase [ubiquinone] 1 alpha 27228985 NDUFA12 subcomplex subunit 12 0,9986 14,1 2 2 1 53 6 13 1 52,3 6 9 1 64,4 7 22 0,000833 282165833 4930526D03Rikuncharacterized protein C20orf201 homolog 0,9986 11,1 2 2 0 0 0 0 0 0 0 0 0,999 11,1 2 2 0,0000601 283046757 HEATR7A HEAT repeat containing 7A isoform 1 0,9986 1,3 2 2 0,9996 1,3 2 2 1 2,6 4 4 1 3,2 5 8 0,0000423 70887605 SPAG16 sperm-associated antigen 16 protein isoform 1 0,9986 3,9 2 3 1 12,4 7 7 1 8,1 4 7 1 11 6 17 0,000226 85702045 LOC100048051multidrug and toxin extrusion protein 2 0,9986 2,8 2 2 0,9775 2,4 1 1 0,9861 2,4 1 1 1 5,2 3 4 0,000106 237820660 UBR4 E3 ubiquitin-protein ligase UBR4 0,9985 0,4 2 2 0,9997 0,4 2 2 0,999 0,4 2 2 1 0,6 3 6 0,0000116 31982845 FOLR1 folate receptor alpha precursor 0,9985 7,5 2 2 0,9708 4,3 1 1 0,9993 10,6 2 2 1 14,9 3 5 0,000263 110625761 LOC100048880AFG3-like protein 2 0,9984 2 2 2 1 15,8 14 14 1 15,1 11 13 1 20,3 16 30 0,000234 50979268 1700065I17Rik uncharacterized protein C5orf48 homolog 0,9984 7,8 1 3 1 18,4 3 3 1 13,5 2 5 1 13,5 2 10 0,000559 83627689 SLC22A14 solute carrier family 22 member 14 0,9984 3,8 2 2 1 6,8 4 4 0,9863 2,4 1 1 1 6,8 4 7 0,000137 377833208 Pdia6 PREDICTED: protein disulfide-isomerase A6 0,9983 12,5 4 5 0,9987 10,7 3 5 0,9998 23,8 7 9 0,9998 23,8 7 19 0,000404 NADH dehydrogenase [ubiquinone] 1 beta subcomplex 225903452 Gm9050 subunit 11, mitochondrial-like 0,9982 11,3 1 2 0,9831 11,3 1 2 0,9992 11,3 1 3 0,9993 11,3 1 7 0,00041 307691260 LOC627607 uncharacterized protein LOC627607 0,9982 4,3 2 2 0,9782 2,5 1 1 0,9992 6,6 2 2 1 10,9 4 5 0,0000729 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX alpha/beta hydrolase domain-containing protein 17B 38142456 Fam108b precursor 0,9982 8,3 2 2 0 0 0 0 0 0 0 0 0,9815 8,3 2 2 - 13385312 PLIN3 perilipin-3 0,9981 5 2 2 1 12,6 3 3 0,9857 3,7 1 1 1 17,6 5 6 0,000117 148747558 PRDX2 peroxiredoxin-2 0,9979 10,6 2 2 1 27,3 5 5 1 48,5 7 11 1 48,5 7 18 0,000574 21728376 Gm6242 myosin regulatory light chain 12B 0,9979 11,6 2 2 0 0 0 0 0,9853 5,8 1 1 1 17,4 3 3 0,000124 227500514 1700080E11Rikuncharacterized protein LOC73532 0,9978 10,2 2 2 0,9993 10,7 2 2 1 32 5 5 1 32 5 8 0,000226 coiled-coil domain-containing protein C16orf93 85986579 Gm166 homolog 0,9978 5,1 2 3 0 0 0 0 0,9533 2,7 1 1 0,9984 5,1 2 4 0,000106 8394493 Tuba8 tubulin alpha-8 chain 0,9976 33 12 11 0 0 0 0 0,9983 34,1 14 39 1 48,1 16 79 0,000966 14192922 Actc1 actin, alpha cardiac muscle 1 0,997 23,9 8 12 1 26,9 9 3 1 33,2 11 4 1 33,2 11 8 0,000121 40556608 HSP90AB1 heat shock protein HSP 90-beta 0,9936 6,6 4 2 0,9995 10,2 7 2 1 14,9 9 5 1 17,5 11 7 0,0000649 13386452 Hist3h2ba histone H2B type 3-A 0,9933 20,6 2 2 0,9936 20,6 2 3 1 27 3 5 1 27 3 9 0,000406 28316750 HIST1H2BA histone H2B type 1-A 0,9933 20,5 2 2 0,9999 27,6 3 4 0,9981 26,8 3 4 1 33,9 4 9 0,000384 100815972 GGH gamma-glutamyl hydrolase precursor 0 0 0 0 1 19,2 5 7 1 15,5 4 5 1 15,5 4 11 0,000274 110224442 TXNRD1 thioredoxin reductase 1, cytoplasmic isoform 2 0 0 0 0 1 7,6 3 3 1 6,6 3 3 1 9,6 4 6 0,0000857 histidine triad nucleotide-binding protein 2, 110625719 HINT2 mitochondrial precursor 0,9721 9,2 1 1 1 29,4 3 3 0,9712 9,2 1 1 1 29,4 3 5 0,000193 110625742 1700007K13RikUPF0691 protein C9orf116 homolog 0,9601 5,4 1 1 1 59,9 6 7 1 40,1 5 6 1 59,3 7 13 0,000796 110625796 4931432M23Rikinactive serine protease 54 precursor 0,9724 1,8 1 1 1 11,7 3 6 1 15,1 4 6 1 19,3 7 13 0,000366 110625979 LOC100047986elongation factor 1-gamma 0,9842 3 1 1 1 17,2 6 10 1 16,9 6 8 1 24,7 9 18 0,000336 110835737 Lcn5 epididymal-specific lipocalin-5 isoform a precursor 0 0 0 0 1 35,9 5 12 1 35,9 5 16 1 35,9 5 26 0,000967 113462019 LOC100047516casein kinase I isoform gamma-3 0 0 0 0 1 9,2 4 5 1 10,6 4 8 0 0 6 0 - 113680352 Gm5678 carbonyl reductase [NADPH] 1 0 0 0 0 1 49,1 10 13 1 35 8 9 1 59,2 12 24 0,000518

113930767 Wfdc15b WAP four-disulfide core domain protein 15B precursor 0,9599 16,2 1 1 1 27,5 2 4 1 27,5 2 4 1 27,5 2 9 0,000685 116063531 CAR1 carbonic anhydrase 1 0,9673 3,8 1 1 1 21,1 5 5 1 45,6 9 9 1 45,6 9 15 0,000534 117606366 CMPK2 UMP-CMP kinase 2, mitochondrial precursor 0 0 0 0 1 10,5 4 4 0,9697 2,7 1 1 1 13,2 5 5 0,000084 118130807 pnpla8 calcium-independent phospholipase A2-gamma 0,9609 1,2 1 1 1 6,1 4 4 1 4,6 3 3 1 8,6 6 8 0,0000739 119508443 DNAJC10 dnaJ homolog subfamily C member 10 precursor 0 0 0 0 1 7,4 5 5 1 5,9 4 4 1 8,7 6 8 0,0000802 124301188 4732415M23Rikuncharacterized protein C16orf55 homolog 0 0 0 0 1 24,2 3 4 1 37,9 4 5 1 37,9 4 8 0,00044 124430564 TCTE1 T-complex-associated testis-expressed protein 1 0,9804 4,2 1 1 1 11,2 4 7 0,9987 3,6 2 3 1 17,3 6 12 0,00024

124486666 EFCAB3 EF-hand calcium-binding domain-containing protein 3 0 0 0 0 1 11,3 4 4 0,9987 6,2 2 2 1 11,3 4 6 0,0000864 124486773 Dnahc2 dynein heavy chain 2, axonemal 0 0 0 0 1 6,9 26 28 1 4 15 14 1 7,8 29 43 0,0000741 124486893 1700009P17RikUPF0740 protein C1orf192 homolog 0,9828 4,8 1 2 1 28 4 6 1 18,5 3 5 1 36 5 13 0,000911 124487360 FAM154A uncharacterized protein LOC75811 0,982 2,1 1 2 1 16,4 7 30 1 20,6 8 28 1 22,9 9 55 0,000991 124487483 Ogdhl 2-oxoglutarate dehydrogenase-like, mitochondrial 0 0 0 0 1 13,2 11 3 1 14,3 12 32 1 19 15 7 0,000054 12746422 XPO7 exportin-7 0 0 0 0 1 3,2 3 3 0,9993 2 2 2 1 3,4 3 4 0,0000313 12963697 Fahd1 acylpyruvase FAHD1, mitochondrial 0,9863 7 1 1 1 28,2 4 5 1 22,9 4 4 1 34,4 5 10 0,000309 13385090 COX6B1 cytochrome c oxidase subunit 6B1 0,9695 17,5 1 1 1 34,9 3 3 1 47,7 4 4 1 47,7 4 8 0,000339 NADH dehydrogenase [ubiquinone] 1 beta subcomplex 13385322 Ndufb7 subunit 7 0 0 0 0 1 46,7 5 13 1 70,8 8 20 1 70,8 8 34 0,001313313 13385592 LYZL1 lysozyme-like protein 1 precursor 0 0 0 0 1 23,6 2 5 0,9994 8,1 1 2 1 23,6 2 7 0,000468 13385700 1700028P14Rikuncharacterized protein C9orf135 homolog 0,9752 5,3 1 1 1 31,6 6 7 1 34,6 7 8 1 39 8 14 0,000309 13385716 1700019N19Rikuncharacterized protein C10orf82 homolog 0 0 0 0 1 25,7 6 12 1 36,3 7 18 1 36,3 7 30 0,001223709 13385800 Tceb2 transcription elongation factor B polypeptide 2 0 0 0 0 1 28,8 4 6 1 22 3 3 1 28,8 4 9 0,000507 serine beta-lactamase-like protein LACTB, 13507666 LOC677144 mitochondrial precursor 0,9828 2,2 1 1 1 8,9 3 3 1 12,9 6 7 1 16,9 7 12 0,000157 14030781 TSSK6 testis-specific serine/threonine-protein kinase 6 0,9799 4 1 1 1 25,3 6 6 1 22 6 6 1 28,6 7 13 0,000303 145966885 SPACA5 sperm acrosome-associated protein 5 precursor 0,9846 6,2 1 1 1 25 4 7 1 13,1 3 4 1 25 4 12 0,000636 149944431 TEKT5 tektin-5 0 0 0 0 1 51,1 20 51 1 41,9 16 44 1 49,2 19 90 0,001379043 157951659 CRISP1 cysteine-rich secretory protein 1 precursor 0 0 0 0 1 21,7 4 7 1 26,2 5 21 1 26,2 5 28 0,000862 158341656 ODF1 outer dense fiber protein 1 0,9552 3,2 1 1 1 40,7 8 35 1 51,2 9 38 1 51,2 9 75 0,001893666 low molecular weight phosphotyrosine protein 159032062 Acp1 phosphatase isoform 1 0 0 0 0 1 20,3 3 3 0 0 0 0 0 0 3 0 - 160298207 GNPAT dihydroxyacetone phosphate acyltransferase 0,9567 1,2 1 1 1 12,5 7 7 1 6 4 4 1 11,2 7 11 0,000114 160333553 rpl12 60S ribosomal protein L12 0 0 0 0 1 43 5 6 1 54,5 6 10 1 54,5 6 17 0,000599 160358825 Apoh beta-2-glycoprotein 1 precursor 0 0 0 0 1 33,6 9 9 1 27,2 7 7 1 33,6 9 16 0,000301

161086922 camk4 calcium/calmodulin-dependent protein kinase type IV 0 0 0 0 1 14,1 5 8 1 7,5 3 8 1 11,5 4 14 0,00026 LETM1 domain-containing protein LETM2, 165905627 LETM2 mitochondrial precursor 0 0 0 0 1 8,1 3 3 0,9994 5 2 2 1 10,4 4 5 0,0000784

171543846 GPX5 epididymal secretory glutathione peroxidase precursor 0,9812 6,3 1 1 1 20,4 4 4 1 25,3 5 6 1 25,3 5 10 0,0003 19526858 4930579J09Rikprotein phosphatase 1 regulatory subunit 32 0 0 0 0 1 40,7 12 17 1 45 13 19 1 48,5 14 36 0,000599 peptidyl-prolyl cis-trans isomerase F, mitochondrial 19527310 PPIF precursor 0 0 0 0 1 20,9 3 3 1 24,8 4 5 1 24,8 4 7 0,000196 19705578 atp6v1b2 V-type proton ATPase subunit B, brain isoform 0 0 0 0 1 7,8 3 3 0,9996 5,1 2 2 1 10 4 5 0,0000687 20149730 BSPRY B box and SPRY domain-containing protein 0 0 0 0 1 21 9 17 1 22,2 10 15 1 27,4 12 33 0,000568 209862939 Hmox2 heme oxygenase 2 0,9628 7,3 1 1 1 38,7 8 8 1 34,9 7 8 1 41 8 16 0,000345 21312276 IMMP1L mitochondrial inner membrane protease subunit 1 0 0 0 0 1 31,3 4 7 1 31,3 4 5 1 31,3 4 12 0,000482 21312546 Fam162a protein FAM162A 0,9717 7,1 1 1 1 23,2 3 5 1 18,7 3 3 1 23,2 4 10 0,000587 21312712 TCEB1 transcription elongation factor B polypeptide 1 0 0 0 0 1 27 2 3 0,9992 27 2 2 1 27 2 5 0,000301 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 21312734 41153 septin-12 isoform 1 0 0 0 0 1 23,7 6 9 1 27,3 7 9 1 27,3 7 18 0,000429 21312812 STARD6 stAR-related lipid transfer protein 6 0,9816 6,4 1 1 1 19,7 3 3 1 19,7 3 3 1 19,7 3 7 0,000199 21313054 IQCD IQ domain-containing protein D 0 0 0 0 1 7,4 3 4 0,9986 4,1 2 2 1 10 4 7 0,0000903 21313146 ENKUR enkurin 0,955 4,7 1 1 1 39,6 11 22 1 39,2 10 33 1 39,6 11 56 0,001354008

21313314 EFCAB1 EF-hand calcium-binding domain-containing protein 1 0 0 0 0 1 15,1 3 5 0,9989 11,8 2 3 1 15,1 3 7 0,000219 21313338 4933411K16Rikuncharacterized protein C10orf62 homolog 0,9595 2,6 1 2 1 17,8 5 10 1 10,5 3 10 1 17,8 5 23 0,000877 small glutamine-rich tetratricopeptide repeat-containing 21313588 Sgta protein alpha 0 0 0 0 1 15,6 4 4 1 18,1 5 5 1 22,9 6 9 0,000239 21389313 4921507P07Rikuncharacterized protein C7orf31 homolog 0 0 0 0 1 18,7 9 16 1 21,4 10 12 1 23,1 12 27 0,000452 214010153 APEH acylamino-acid-releasing enzyme 0,9765 1,5 1 1 1 9 4 4 1 11,2 5 5 1 15,4 6 10 0,000129 21489937 lancl1 lanC-like protein 1 0,9863 3 1 2 1 7,8 3 3 0,9861 3 1 2 0,9995 5 2 6 0,000137 alpha/beta hydrolase domain-containing protein 11 21644577 abhd11 isoform 1 0 0 0 0 1 17,2 2 3 0,9575 11 1 1 1 28,2 3 3 0,000114 21699068 PITRM1 presequence protease, mitochondrial precursor 0 0 0 0 1 20,1 16 20 1 25,4 19 26 1 27,9 21 44 0,000343 21703810 Ubl4 ubiquitin-like protein 4A 0 0 0 0 1 24,8 3 3 0,9709 5,1 1 1 1 24,8 3 4 0,000161 CKLF-like MARVEL transmembrane domain- 21729765 Cmtm2b containing protein 2B 0 0 0 0 1 7,6 2 3 0,9633 3,8 1 2 0,9999 7,6 2 7 0,000349 22094075 LOC631229 ADP/ATP translocase 2 0 0 0 0 1 20,1 5 27 1 26,5 7 9 1 26,5 7 13 0,000263

225579120 Pdzk1 Na(+)/H(+) exchange regulatory cofactor NHE-RF3 0 0 0 0 1 10,4 5 5 1 12,7 6 6 1 19,7 9 12 0,000129

225690614 ACOT7 cytosolic acyl coenzyme A thioester hydrolase isoform 2 0,9777 3,4 1 1 1 15,6 4 4 1 14,2 4 7 1 18,5 5 12 0,000272 226442740 40422 septin-10 isoform 1 0,9851 2,8 1 2 1 27,7 8 12 1 29,3 9 12 1 40 12 26 0,000435 226693361 CCT6B T-complex protein 1 subunit zeta-2 0,9776 7,9 4 1 1 16,4 7 4 1 18,3 8 5 1 22,6 10 10 0,000129 23346595 2410015M20Rikprotein QIL1 precursor 0 0 0 0 1 41,2 3 4 1 41,2 3 4 1 41,2 3 9 0,000714 237874196 Dnahc7a dynein, axonemal, heavy chain 7B 0,9726 0,2 1 1 1 14,1 50 23 1 11,1 38 14 1 15,6 53 35 0,0000632 244790085 CCDC81 coiled-coil domain-containing protein 81 0,9586 1,7 1 1 1 7,5 4 11 1 8,7 5 14 1 8,7 5 26 0,000254 251823732 1700019B03Rikuncharacterized protein LOC76406 0 0 0 0 1 8,2 3 3 0,9994 4,8 2 2 1 8,2 3 4 0,0000585 iron-sulfur cluster assembly 2 homolog, mitochondrial 254039596 iscA2 precursor 0,9646 7,8 1 1 1 20,8 3 3 0,9858 7,8 1 1 0,9997 15,6 2 4 0,000129 254675296 PROM1 prominin-1 isoform s1 precursor 0,9706 1,4 1 1 1 11,1 7 9 1 7,3 4 6 1 14,3 9 18 0,000204 254692843 Dnahc10 dynein heavy chain 10, axonemal 0,9842 0,3 1 1 1 7,9 32 34 1 3,1 14 14 1 7,8 32 46 0,0000692 255003729 EFHB EF-hand domain-containing family member B 0 0 0 0 1 16,2 11 15 1 21,6 13 16 1 23,4 15 28 0,000249 255759902 MAOA amine oxidase [flavin-containing] A 0,9657 1,7 1 1 1 6,7 3 5 1 12 5 7 1 14,1 6 14 0,000188 255759941 Serpinb6a serpin B6 isoform a 0 0 0 0 1 11,1 3 3 1 14 5 6 1 21,2 7 10 0,000179 256773234 Dnahc1 dynein, axonemal, heavy chain 1 0 0 0 0 1 9,8 34 35 1 9,4 29 32 1 12,4 41 65 0,000124 262263372 ahcY adenosylhomocysteinase 0 0 0 0 1 26 5 5 1 24,8 8 8 1 29,4 10 14 0,000213 268370283 Gm5429 uncharacterized protein C7orf72 homolog 0 0 0 0 1 39,6 4 5 1 34,9 3 5 1 34,9 3 8 0,000482 27369597 A330021E22Rikuncharacterized protein C7orf63 homolog 0,9656 1,5 1 1 1 4,8 4 5 1 4,1 4 6 1 5,3 5 10 0,0000828 27370032 UBA6 ubiquitin-like modifier-activating enzyme 6 0,9616 1,3 1 1 1 4,5 4 4 1 9,4 7 7 1 10,4 8 13 0,0000932 28077051 NLN neurolysin, mitochondrial precursor 0 0 0 0 1 7,8 4 4 1 8,1 4 5 1 8,1 4 8 0,0000893 NADH dehydrogenase [ubiquinone] iron-sulfur protein 281485615 NDUFS4 4, mitochondrial 0,9618 5,7 1 1 1 30,9 5 9 1 30,9 5 10 1 30,9 5 21 0,000646 281604088 Gm7655 isopentenyl-diphosphate Delta-isomerase 1 0 0 0 0 1 14,1 3 3 1 11,7 3 3 1 18,4 4 7 0,000164 282721066 4931409K22RikIQ and AAA domain-containing protein 1-like 0,9815 1,5 1 5 1 4,2 3 5 1 4,2 3 7 1 4,2 3 17 0,000124 282721112 CASC1 cancer susceptibility candidate protein 1 0 0 0 0 1 11 7 13 1 8,8 6 15 1 10,3 7 27 0,000273 monofunctional C1-tetrahydrofolate synthase, 283135110 MTHFD1L mitochondrial precursor 0,9564 1 1 1 1 7,9 6 6 1 13,6 11 12 1 14,8 12 19 0,000136 284172505 1700007I08Rik actin-like protein 10 0,9823 5,8 1 1 1 17,1 4 5 1 27,7 6 8 1 27,7 6 14 0,00036 29126205 ACAA2 3-ketoacyl-CoA thiolase, mitochondrial 0,9818 3,5 1 1 1 11,8 3 3 1 19,9 6 6 1 19,9 6 10 0,000197 293597569 Aldoa2 fructose-bisphosphate aldolase A isoform 2 0 0 0 0 1 81,9 24 41 0,9999 79,9 21 44 0,9999 79,9 22 116 0,001438588 295054193 Odf2 outer dense fiber protein 2 isoform e 0 0 0 0 1 49,6 33 328 0,9726 46,2 31 1 1 49,6 33 689 0,004393161 putative transferase CAF17 homolog, mitochondrial 30424663 A230051G13Rikisoform 1 precursor 0,9838 3,6 1 1 1 10,1 3 3 0,9994 7 2 2 1 10,1 3 5 0,000125 30425280 4930412F15Rikprotein FAM221B 0,9556 2,5 1 1 1 8,6 3 4 1 9,2 3 4 1 12,3 4 9 0,000156 307691211 - regulator of G-protein signaling 22 0 0 0 0 1 5,7 7 8 1 8,9 11 11 1 10,6 13 18 0,000113 308082030 Styxl1 serine/threonine/tyrosine-interacting-like protein 1 0,9695 4,7 1 1 1 17,1 4 4 1 17,1 4 6 1 17,1 4 11 0,000241 PREDICTED: uncharacterized protein C17orf47 309262380 LOC100862214homolog 0,983 2,1 1 2 1 12,3 7 26 1 10,4 6 19 1 15,7 9 47 0,000706 309264486 fsip2 PREDICTED: fibrous sheath-interacting protein 2 0 0 0 0 1 6 35 71 1 6,3 35 79 1 7,1 40 141 0,000228 309267418 Dnah7 PREDICTED: dynein heavy chain 7, axonemal 0 0 0 0 1 12,9 45 16 1 9,8 33 8 1 15 50 23 0,0000411

31543001 TBL1XR1 F-box-like/WD repeat-containing protein TBL1XR1 0 0 0 0 1 9,9 3 3 1 9,5 3 3 1 13 4 6 0,000114

31543330 Nnt NAD(P) transhydrogenase, mitochondrial precursor 0 0 0 0 1 12 11 13 1 14,3 11 12 1 18,5 15 24 0,000247 319655563 Ccdc136 coiled-coil domain-containing protein 136 isoform 2 0 0 0 0 1 12,8 7 9 1 11,3 6 9 1 14,8 8 18 0,00016

31982133 AMDHD2 putative N-acetylglucosamine-6-phosphate deacetylase 0,986 3,4 1 2 1 21 5 6 1 21 5 6 1 24,4 6 14 0,000305 31982273 hsd17b4 peroxisomal multifunctional enzyme type 2 0 0 0 0 1 10,5 6 6 1 21,8 11 16 1 23,1 12 21 0,000212 33468873 ACE angiotensin-converting enzyme isoform 2 precursor 0 0 0 0 1 28 17 38 1 23,9 14 32 0 0 18 0 - 33859482 Gm13050 elongation factor 2 0,9769 1,3 1 1 1 4,1 3 3 0,9856 1,3 1 1 1 4,1 3 5 0,0000372 33942057 SPINT4 kunitz-type protease inhibitor 4 precursor 0,9558 4,4 1 1 1 25,2 3 4 0,9993 17,6 2 4 1 25,2 3 9 0,000401 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 34328334 Acox3 peroxisomal acyl-coenzyme A oxidase 3 0,9659 2,1 1 1 1 18,9 10 14 1 22 11 17 1 22 11 29 0,000297 354983491 Dnahc7a dynein, axonemal, heavy chain 7A 0 0 0 0 1 12,8 44 5 0,9999 9,9 33 3 1 14,5 48 8 0,0000144 363543275 Adgb androglobin 0 0 0 0 1 4,5 6 6 1 4,8 6 6 1 5,9 7 12 0,0000604 PREDICTED: orphan sodium- and chloride-dependent 377834273 Ntt5 neurotransmitter transporter NTT5 0,9849 1,7 1 1 1 9,4 4 6 0,9995 5,8 2 4 1 10,7 5 12 0,000292

377834275 - PREDICTED: uncharacterized protein LOC100862214 0 0 0 0 1 10,6 3 3 1 15,5 4 5 1 15,5 4 8 0,000234 38348576 Odf3l1 outer dense fiber protein 3-like protein 1 0,9831 4,4 1 1 1 16,4 4 7 0,9998 10,2 2 3 1 16,4 4 11 0,000326 40254507 PGP phosphoglycolate phosphatase 0 0 0 0 1 12,1 3 3 1 12,1 3 3 1 12,1 3 6 0,00012 406855427 Xpnpep1 xaa-Pro aminopeptidase 1 0 0 0 0 1 5,1 3 3 1 7,1 4 4 1 9,2 5 8 0,0000814 407262105 Dnhc12 PREDICTED: dynein heavy chain 12, axonemal 0 0 0 0 1 9,6 32 39 1 7 21 24 1 11,9 38 61 0,000111 40807498 Hrsp12 ribonuclease UK114 0,9793 7,4 1 1 1 29,6 3 3 0,9857 10,4 1 1 1 37 4 5 0,000191 42415475 p4hb protein disulfide-isomerase precursor 0,9863 3,1 1 1 1 9,8 4 5 1 16,3 7 8 1 18,3 8 13 0,000145 46049022 PSMD6 26S proteasome non-ATPase regulatory subunit 6 0 0 0 0 1 11,8 4 4 1 12,6 5 6 1 15,2 6 9 0,000127 46485467 SPATA20 spermatogenesis-associated protein 20 0 0 0 0 1 6,3 5 5 0,9998 3,2 2 2 1 6,3 5 8 0,0000834 serine/threonine-protein phosphatase 2A 56 kDa 47059051 Gm13169 regulatory subunit alpha isoform 0 0 0 0 1 10,7 4 4 0 0 0 0 1 10,7 4 4 0,0000639 47564127 BC048507 dynein light chain LC8-type 1-like 0,9816 24,7 2 1 1 34,8 4 3 0,9993 42,7 4 2 1 42,7 4 7 0,000399 52138534 BC048562 uncharacterized protein LOC434439 0,9683 6,9 1 2 1 41,7 5 7 1 30,4 4 6 1 41,7 5 15 0,000863 N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase 54292135 aga isoform 1 precursor 0 0 0 0 1 22,3 5 6 1 29,8 7 13 1 29,8 7 19 0,000481 58037189 1700026L06Rikuncharacterized protein C9orf9 homolog 0,9863 7,7 1 3 1 25 3 11 1 20,2 2 9 1 25 3 23 0,001100807 58037271 1700023E05Riktesticular haploid expressed gene protein-like 0,9723 1,5 1 1 1 16,8 6 11 1 20,9 7 12 1 24,2 9 22 0,000294 58037363 SPATC1 speriolin 0,9698 3,5 1 3 1 22,1 6 18 1 26,2 7 30 1 26,2 7 51 0,001259147 58037409 Phpt1 14 kDa phosphohistidine phosphatase 0 0 0 0 1 19,4 2 4 0,9996 9,7 1 2 1 19,4 2 6 0,000283 6679337 pitpna phosphatidylinositol transfer protein alpha isoform 0 0 0 0 1 11,8 2 3 0,9841 8,5 1 1 1 11,8 2 5 0,000116 6679439 Gm9234 peptidyl-prolyl cis-trans isomerase A 0 0 0 0 1 29,3 4 7 1 37,8 5 8 1 42,1 6 14 0,000457 6679505 PSMD4 26S proteasome non-ATPase regulatory subunit 4 0 0 0 0 1 11,4 3 3 1 11,7 3 4 1 15,4 4 7 0,000198 6680604 Krt16 keratin, type I cytoskeletal 16 0 0 0 0 1 16 7 5 0 0 0 0 1 16 7 4 0,0000614 6680724 Arf6 ADP-ribosylation factor 6 0,9674 6,3 1 1 1 33,1 4 5 1 31,4 4 7 1 43,4 5 13 0,000513 6680924 CFL1 cofilin-1 0 0 0 0 1 36,1 4 3 0,9995 22,3 3 3 1 44,6 5 7 0,000204 67514549 hexA beta-hexosaminidase subunit alpha precursor 0,961 2,1 1 1 1 6,2 3 3 1 6,2 3 3 1 8,3 4 7 0,000125 6753036 ALDH2 aldehyde dehydrogenase, mitochondrial precursor 0 0 0 0 1 12,5 5 5 1 16,8 6 6 1 21,4 8 11 0,000149 6754976 Gm7204 peroxiredoxin-1 0 0 0 0 1 27,1 6 6 1 40,2 8 10 1 40,2 8 18 0,000519 6755714 TAGLN transgelin 0 0 0 0 1 18,9 3 4 1 38,8 8 22 1 38,8 8 26 0,000758 6755863 Hsp90b1 endoplasmin precursor 0,9843 2,6 2 1 1 6,1 4 7 1 14,3 10 11 1 16,1 11 20 0,000164 voltage-dependent anion-selective channel protein 3 6755967 Gm7591 isoform 2 0 0 0 0 1 53 11 23 1 50,2 10 22 1 53 11 50 0,001063872 7106389 PSMA7 proteasome subunit alpha type-7 0,987 25 4 1 1 36,3 7 10 0,9869 25,4 5 2 1 36,3 7 6 0,000156 75992911 Acsl6 long-chain-fatty-acid--CoA ligase 6 isoform 1 0 0 0 0 1 12,2 6 6 0,9994 4 2 2 1 13,9 7 8 0,0000785 7656912 ATP8A2 probable phospholipid-transporting ATPase IB 0,9714 1,1 1 1 1 2,9 3 3 0,9996 2,2 2 2 1 4 4 7 0,0000499 83999999 Gnpda2 glucosamine-6-phosphate isomerase 2 0 0 0 0 1 18,5 4 4 1 29 6 7 1 29 6 11 0,000257 85702101 Akap14 A-kinase anchor protein 14 0 0 0 0 1 5,6 3 3 1 7,6 4 4 1 9,5 5 8 0,000106 87162476 ccdc108 coiled-coil domain-containing protein 108 0 0 0 0 1 2,5 5 5 1 3,7 7 8 1 3,7 7 11 0,0000655 88759352 glyctk glycerate kinase 0 0 0 0 1 10,3 4 4 0,9692 1,3 1 1 1 8,6 3 4 0,0000837 93102417 Gars glycine--tRNA ligase 0 0 0 0 1 5,5 3 3 0,9619 1,4 1 1 1 6,9 4 4 0,0000365 96975138 hprT hypoxanthine-guanine phosphoribosyltransferase 0 0 0 0 1 17,4 3 3 1 16,5 3 3 1 22 4 7 0,000183

9790123 NME4 nucleoside diphosphate kinase, mitochondrial precursor 0 0 0 0 1 34,9 5 5 1 23,1 3 3 1 34,9 5 7 0,000357 13384742 Gm10078 28S ribosomal protein S36, mitochondrial isoform 1 0,9636 23,5 1 1 0,9999 56,9 3 3 0,9995 36,3 2 2 1 56,9 3 5 0,000671

15011842 Smcp sperm mitochondrial-associated cysteine-rich protein 0 0 0 0 0,9999 14,2 2 9 0,9998 14,2 2 7 0,9998 14,2 2 16 0,001613096 160333218 BEST1 bestrophin-1 0 0 0 0 0,9999 5,1 2 2 0,9859 3,1 1 1 0,9998 5,1 2 4 0,0000666 spermatogenesis-associated protein 19, mitochondrial 19526759 SPATA19 precursor 0,9794 9,7 1 1 0,9999 9,7 1 5 1 31,2 3 8 1 31,2 3 14 0,000614 225579132 Txndc2 thioredoxin domain-containing protein 2 isoform 2 0 0 0 0 0,9999 3,7 2 2 0,9843 2 1 1 0,9998 3,7 2 3 0,0000394 86198335 NUDT2 bis(5'-nucleosyl)-tetraphosphatase [asymmetrical] 0 0 0 0 0,9999 21,1 2 2 1 27,2 3 3 1 36,7 4 5 0,000199 9789937 Dnaja2 dnaJ homolog subfamily A member 2 0,978 1,7 1 2 0,9999 4,9 2 2 0,9997 4,9 2 2 0,9998 4,9 2 6 0,000101 10946854 Gm5081 ribose-phosphate pyrophosphokinase 1 0,9832 3,8 1 1 0,9998 7,9 2 2 1 21,1 5 5 1 25,2 6 8 0,00018 124001554 CATSPER2 cation channel sperm-associated protein 2 0,9828 1,9 1 1 0,9998 1,9 1 2 0,9993 4,4 2 2 1 7 3 7 0,000141 guanine nucleotide-binding protein G(I)/G(S)/G(T) 13937391 gnb2 subunit beta-2 0,9807 2,9 1 1 0,9998 6,2 2 2 0,977 2,9 1 1 0,9997 6,2 2 4 0,0000931 149258833 Gm16486 PREDICTED: uncharacterized protein KIAA1683 0,9776 0,7 1 1 0,9998 2,2 2 4 1 4,7 5 8 1 5,7 6 12 0,000105 154090941 Krt42 keratin, type I cytoskeletal 42 0 0 0 0 0,9998 10,2 5 2 0 0 0 0 0,9998 10,2 5 2 0,0000339 157951602 Wfdc16 WAP four-disulfide core domain 16 0 0 0 0 0,9998 11,7 1 2 0,9832 11,7 1 1 0,9997 11,7 1 3 0,00044 160358385 ART3 ecto-ADP-ribosyltransferase 3 precursor 0 0 0 0 0,9998 8,6 2 2 0,971 6,6 1 1 1 15,2 3 3 0,0000728 165972327 Hdhd1a pseudouridine-5'-monophosphatase 0,9836 7,3 1 2 0,9998 15,4 2 5 1 26,1 3 8 1 34,2 4 15 0,000811

167716841 COX3 ATP synthase F0 subunit 8 [Mus musculus musculus] 0,9704 14,9 1 3 0,9998 34,3 2 4 0,9995 34,3 2 4 0,9997 34,3 2 11 0,001617348

19072792 ERP44 endoplasmic reticulum resident protein 44 precursor 0 0 0 0 0,9998 8,6 2 2 0 0 0 0 0,9998 8,6 2 2 0,0000403 21312153 glrx5 glutaredoxin-related protein 5, mitochondrial 0,984 9,2 1 1 0,9998 21,7 2 2 0,9844 9,2 1 1 1 21,7 2 4 0,00021 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX iron-sulfur cluster assembly 1 homolog, mitochondrial 21312189 iscA1 precursor 0 0 0 0 0,9998 19,4 2 3 0,9996 19,4 2 2 0,9998 19,4 2 5 0,000206 251823962 4930435E12Rikuncharacterized protein LOC74663 0,958 2,4 1 1 0,9998 7,1 2 4 0,9997 7,1 2 5 0,9997 7,1 2 10 0,000229 254675129 ANKRD45 ankyrin repeat domain-containing protein 45 0 0 0 0 0,9998 9,6 2 3 0,9772 3,9 1 1 0,9997 9,6 2 4 0,000107 34304065 FAM24A protein FAM24A precursor 0 0 0 0 0,9998 22,4 2 2 0,9802 10,2 1 1 0,9998 22,4 2 3 0,000241 357933645 Ptgr2 prostaglandin reductase 2 isoform 1 0 0 0 0 0,9998 8 2 2 1 10,8 3 3 1 13,7 4 5 0,000128 37700232 Nme1 nucleoside diphosphate kinase A 0 0 0 0 0,9998 31,6 4 9 1 56,6 7 8 1 56,6 7 12 0,000552 6754696 Gm16379 macrophage migration inhibitory factor 0,9712 9,6 1 1 0,9998 9,6 1 2 0,9855 9,6 1 1 0,9997 9,6 1 4 0,000402 6806917 gm2a ganglioside GM2 activator precursor 0,9785 5,2 1 1 0,9998 10,4 2 2 0,9996 10,4 2 2 0,9998 10,4 2 5 0,000254 hydroxyacyl-coenzyme A dehydrogenase, mitochondrial 111038118 hadh precursor 0 0 0 0 0,9997 5,7 2 2 0,9994 5,7 2 2 0,9996 5,7 2 4 0,000101 124107621 1700029F12Rikuncharacterized protein LOC66479 isoform 1 0 0 0 0 0,9997 10,2 1 2 0,9659 10,2 1 1 0,9996 10,2 1 3 0,000266 125347370 TEX13A testis-expressed sequence 13A protein 0,9861 3,4 1 1 0,9997 6,6 2 2 0,9996 6,6 2 2 0,9997 6,6 2 5 0,000106 13385392 napA alpha-soluble NSF attachment protein 0 0 0 0 0,9997 7,5 2 2 1 16,3 4 4 1 20,3 5 6 0,00012 133922575 NUDCD2 nudC domain-containing protein 2 0 0 0 0 0,9997 17,2 2 2 1 34,4 4 4 1 34,4 4 6 0,00028 15277319 Gm6776 leucine zipper transcription factor-like protein 1 0,9791 3,7 1 1 0,9997 10 2 2 0 0 0 0 1 13,7 3 3 0,000072 158854016 Skp1a S-phase kinase-associated protein 1 0,9772 7,4 1 1 0,9997 15,3 2 3 0,9863 7,4 1 1 0,9998 15,3 2 5 0,000227 168693651 Dnalc4 dynein light chain 4, axonemal 0 0 0 0 0,9997 20 2 2 1 29,5 3 4 1 29,5 3 6 0,000356 21536248 GSTT3 glutathione S-transferase, theta 3 0,9659 5,4 1 1 0,9997 13,3 2 3 1 16,6 3 5 1 16,6 3 9 0,000291 229093139 HTRA1 serine protease HTRA1 precursor 0,9682 2,5 1 1 0,9997 5 2 4 1 12,9 4 8 1 15 5 13 0,000212 254540068 KRT18 keratin, type I cytoskeletal 18 0,9577 8 3 1 0,9997 9,9 4 2 1 16,3 5 4 1 22,9 7 6 0,0000804 267844862 1700011H22Rikizumo sperm-egg fusion protein 3 precursor 0,9845 5,3 1 4 0,9997 10,3 2 5 0,9995 10,3 2 5 1 13,3 3 16 0,00049 31543952 WARS2 tryptophan--tRNA ligase, mitochondrial precursor 0 0 0 0 0,9997 5,3 2 2 1 11,9 4 4 1 11,9 4 6 0,000115 serine/threonine-protein phosphatase PP1-gamma 31980772 LOC627816 catalytic subunit 0,9566 23,2 6 1 0,9997 30,3 8 5 1 39,6 10 9 1 42,1 11 17 0,000372 34328471 RALA ras-related protein Ral-A precursor 0 0 0 0 0,9997 12,1 2 2 1 28,2 5 5 1 28,2 5 7 0,000255 PREDICTED: membrane-spanning 4-domains 407264262 Ms4a6b subfamily A member 14 0,9635 3,3 1 1 0,9997 4,4 2 3 0,9705 1,1 1 1 0,9994 4,4 2 5 0,000113 58037419 1700018L24Riktestis-expressed sequence 29 protein 0,9817 4,3 1 4 0,9997 11,8 2 4 1 19,4 3 5 1 19,4 3 13 0,000618 72384361 GSK3A glycogen synthase kinase-3 alpha 0,982 2 1 1 0,9997 5,3 2 2 0,9993 4,9 2 3 1 8,2 3 6 0,000122 90093343 1700052N19RikUPF0364 protein C6orf211 homolog 0,9757 2,5 1 2 0,9997 5 2 2 1 9,6 4 4 1 9,6 4 8 0,000175 113374150 1700016C15Rikuncharacterized protein C1orf100 homolog 0 0 0 0 0,9996 17,2 2 3 1 22,8 3 4 1 22,8 3 7 0,000433 12963757 isyna1 inositol-3-phosphate synthase 1 0 0 0 0 0,9996 3,6 2 2 1 6,8 4 4 1 6,8 4 7 0,000143 148232950 Pgcp carboxypeptidase Q precursor 0 0 0 0 0,9996 5,1 2 2 0,9997 5,1 2 3 0,9998 5,1 2 5 0,0000955 15011843 SEPHS2 selenide, water dikinase 2 0,9587 1,5 1 1 0,9996 6,6 2 2 0,9987 4,9 2 2 1 8,2 3 5 0,00017 198278509 4930588N13Riktestis-expressed sequence 26 protein 0 0 0 0 0,9996 7,8 2 2 0 0 0 0 0,9994 7,8 2 2 0,0000621 21311897 ASB9 ankyrin repeat and SOCS box protein 9 0,9675 3,8 1 1 0,9996 7,9 2 2 0,9975 7,9 2 2 0,9994 7,9 2 5 0,0002 1-phosphatidylinositol 4,5-bisphosphate 22550102 PLCZ1 phosphodiesterase zeta-1 0,9863 1,9 1 1 0,9996 4 2 2 0 0 0 0 0,9998 4 2 3 0,0000464 succinate dehydrogenase cytochrome b560 subunit, 228008307 sdhC mitochondrial precursor 0,9608 4,7 1 1 0,9996 12,4 2 2 0,9994 12,4 2 3 0,9995 12,4 2 6 0,000375 disintegrin and metalloproteinase domain-containing 229332006 ADAM32 protein 32 precursor 0 0 0 0 0,9996 2,5 2 2 0 0 0 0 0,9994 2,5 2 2 0,0000229 254553287 Tmem89 transmembrane protein 89 precursor 0 0 0 0 0,9996 11,8 2 4 0 0 0 0 0,9995 11,8 2 4 0,000319 254588112 1700021F07Rikuncharacterized protein C20orf85 homolog 0 0 0 0 0,9996 15,4 2 3 0,9835 8,8 1 1 0,9996 15,4 2 4 0,000233 256773297 Gm7684 E3 ubiquitin-protein ligase MARCH5 isoform 1 0 0 0 0 0,9996 11,9 2 3 1 18,4 3 3 1 18,4 3 5 0,000124 268839833 TTC29 tetratricopeptide repeat protein 29 0,9715 3,4 1 2 0,9996 4,9 2 2 0,9992 6,2 2 2 1 7,6 3 6 0,0000825 27229125 COASY bifunctional coenzyme A synthase precursor 0 0 0 0 0,9996 4,1 2 2 0,9699 1,6 1 1 1 5,7 3 3 0,0000643 28395018 JUP junction plakoglobin 0 0 0 0 0,9996 3,5 2 2 0 0 0 0 0,9995 3,5 2 2 0,0000323 316659405 - prostate and testis expressed precursor 0 0 0 0 0,9996 20 2 2 0,9993 20 2 3 0,9994 20 2 5 0,000199 33859765 ERI3 ERI1 exoribonuclease 3 0,9703 2,7 1 1 0,9996 6,2 2 2 0,9996 6,2 2 2 0,9996 6,2 2 5 0,000174 spermatid-specific manchette-related protein 1 isoform 359339012 Smrp1 3 0 0 0 0 0,9996 52 14 2 0,9819 45,9 12 2 0,9834 51,7 14 3 - 63079689 Tbata protein TBATA isoform 2 0 0 0 0 0,9996 9,2 2 3 0,9603 4,2 1 1 0,9991 9,2 2 4 0,000109 6679863 FXN frataxin, mitochondrial precursor 0 0 0 0 0,9996 11,1 2 3 1 19,3 3 3 1 25,1 4 6 0,000191 84993780 Defb47 beta-defensin 47 precursor 0 0 0 0 0,9996 24,1 2 2 0 0 0 0 0,9994 24,1 2 2 0,000111 9910416 DNAJB8 dnaJ homolog subfamily B member 8 0,9672 4,4 1 1 0,9996 10,1 2 2 0,9997 10,1 2 2 0,9997 10,1 2 5 0,000169 114326546 pgam1 phosphoglycerate mutase 1 0,978 18,1 5 1 0,9995 22,4 6 2 0,9996 25,2 6 2 0,9997 25,2 6 4 0,00011 13385842 4921517D21Rikuncharacterized protein LOC67722 0 0 0 0 0,9995 2,1 2 2 0,9995 1,8 2 7 1 2,9 3 9 0,0000756 160420337 ENO4 enolase-like protein ENO4 0,9848 1,8 1 1 0,9995 3,9 2 2 0,9995 3,9 2 3 0,9997 3,9 2 5 0,0000815 167736371 ube4a ubiquitin conjugation factor E4 A 0 0 0 0 0,9995 2,5 2 2 1 3,9 3 3 1 5,1 4 4 0,0000448 19882207 LOC674674 farnesyl pyrophosphate synthase isoform 2 0,9817 2,5 1 1 0,9995 4,8 2 2 0,9998 6,5 2 2 1 8,8 3 5 0,000117 transmembrane emp24 domain-containing protein 10 21312062 tmed10 precursor 0 0 0 0 0,9995 12,8 2 2 1 20,1 3 5 1 27,9 4 7 0,000274

237649068 Wfdc6b WAP four-disulfide core domain protein 6B precursor 0 0 0 0 0,9995 11,5 2 2 0 0 0 0 0,9993 11,5 2 2 0,000113 238776835 BTBD16 BTB/POZ domain-containing protein 16 0 0 0 0 0,9995 3,3 2 2 0,9791 2,3 1 1 1 5,6 3 3 0,0000433 241982800 1700080O16Rikuncharacterized protein LOC74279 0,9832 3,9 1 3 0,9995 7 2 2 1 20,8 5 6 1 20,8 5 12 0,000299 55925585 Spag8 sperm-associated antigen 8 0 0 0 0 0,9995 6,3 2 2 0,9993 6,3 2 2 0,9996 6,3 2 4 0,000108 6680720 ARF4 ADP-ribosylation factor 4 0,9526 11,7 2 1 0,9995 41,1 5 2 0,9997 43,9 5 2 1 49,4 6 5 0,000201 188528613 Gmfb glia maturation factor beta 0 0 0 0 0,9994 14,1 2 2 0,9808 7,7 1 1 0,9858 7,7 1 2 - 198278559 1700013F07Rikuncharacterized protein C1orf194 homolog 0 0 0 0 0,9994 16,1 2 3 0,9712 10,1 1 1 1 26,2 3 4 0,000168 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX glycerophosphodiester phosphodiesterase domain- 23956140 gdpd1 containing protein 1 0 0 0 0 0,9994 6,4 2 2 0 0 0 0 1 11,5 3 4 0,000082 31543845 Tbl2 transducin beta-like protein 2 precursor 0,9688 2,9 1 2 0,9994 5 2 2 0,9847 2,9 1 1 0,9858 2,9 1 4 - 33859640 taldo1 transaldolase 0 0 0 0 0,9994 5,6 2 2 0,9689 2,4 1 1 0,9994 5,6 2 5 0,000086 54607171 KRT6A keratin, type II cytoskeletal 6A 0 0 0 0 0,9994 9,9 5 2 0 0 0 0 0,9989 11,4 6 2 0,0000347 coenzyme Q-binding protein COQ10 homolog B, 89337266 Gm4899 mitochondrial isoform 2 0 0 0 0 0,9994 14,1 2 2 1 19,3 3 3 1 19,3 3 5 0,000156 78711830 Adrbk2 beta-adrenergic receptor kinase 2 isoform 1 0 0 0 0 0,9993 2,9 2 2 0 0 0 0 0,999 2,9 2 2 0,0000208 13937355 Gm2904 S-formylglutathione hydrolase 0,9721 7,8 1 1 0,9992 12,1 2 2 0,9994 12,1 2 2 0,9997 12,1 2 5 0,000142 31981588 ATP6V1H V-type proton ATPase subunit H 0 0 0 0 0,9992 7,5 2 2 0,9793 2,7 1 1 0,9995 7,5 2 2 0,0000303

226823223 Ube2v2 ubiquitin-conjugating enzyme E2 variant 2 isoform 2 0 0 0 0 0,9991 16,5 2 2 0 0 0 0 0 0 1 0 - 323276641 Crisp2 cysteine-rich secretory protein 2 precursor 0 0 0 0 0,9991 10,7 2 2 0,9615 4,5 1 1 0,9989 10,7 2 3 0,000115 6755152 prep prolyl endopeptidase 0,9792 2,4 1 1 0,9991 4,1 2 2 1 5,4 3 3 1 7 4 6 0,0000554 89337262 FLAD1 FAD synthase 0 0 0 0 0,9991 6,9 2 2 0,9991 4,1 2 2 1 8,9 3 4 0,000054 PREDICTED: sperm head and tail associated protein- 407261206 Nsun4 like 0 0 0 0 0,999 4 2 2 0,9993 3,1 2 2 1 5,6 3 5 0,0000853 reticulon-4-interacting protein 1, mitochondrial 31543608 RTN4IP1 precursor 0 0 0 0 0,9989 10,9 2 2 0 0 0 0 0,9986 7,6 2 2 0,0000327 31560697 H1f0 histone H1.0 0,9587 4,6 1 1 0,9988 13,4 2 2 0,9669 5,2 1 1 1 23,2 4 4 0,000198 190844347 OAZ3 ornithine decarboxylase antizyme 3 0 0 0 0 0,9987 4,9 1 2 0 0 0 0 0,9672 4,9 1 1 - 226442937 IQCF1 IQ domain-containing protein F1 isoform 1 0,9853 10,9 1 1 0,9987 18,1 2 2 0,9996 20,3 2 2 0,9997 20,3 2 4 0,000214 70778812 TMEM65 transmembrane protein 65 0 0 0 0 0,9986 9 2 2 0,9805 4,7 1 1 0,9993 9 2 3 0,000105 45476581 SCP2 non-specific lipid-transfer protein 0 0 0 0 0,9985 3,1 2 2 1 6,9 4 4 1 9,1 5 7 0,00011 46430514 4930504O13Rikuncharacterized protein LOC403200 precursor 0,9599 4,8 1 1 0,9985 10,3 2 2 1 23,4 4 4 1 23,4 4 7 0,000356 117553604 CES3 carboxylesterase 1D precursor 0,9764 1,8 1 1 0,9984 3,7 2 2 1 6 3 4 1 7,8 4 7 0,000117 160298168 Gbas protein NipSnap homolog 2 0 0 0 0 0,9983 7,8 2 2 0,9987 9,3 2 2 0,9999 14,2 3 4 0,000138 21313200 lyrm1 LYR motif-containing protein 1 0,9645 13,1 1 1 0,9982 14,8 2 2 0,9713 13,1 1 1 0,9989 20,5 2 3 0,000169 mitochondrial inner membrane organizing system 244791232 2310028O11Rikprotein 1 0,96 10,5 1 1 0,9982 10,5 1 2 0 0 0 0 0,9984 10,5 1 4 0,000377 NADH dehydrogenase [ubiquinone] 1 beta subcomplex 84781779 NDUFB6 subunit 6 0 0 0 0 0,9982 11,7 2 2 0,999 19,5 2 4 1 25,8 3 6 0,000294 264681530 - predicted gene, 17727 precursor 0 0 0 0 0,9981 23,9 2 2 0,9717 15,6 1 2 0,999 23,9 2 4 0,000301 226958354 PPM1B protein phosphatase 1B isoform 1 0,9544 5,4 2 1 0,9971 10,3 3 3 0 0 0 0 0,9999 12,6 4 7 0,000113 28076911 Gstm4 glutathione S-transferase mu 4 isoform 1 0 0 0 0 0,9929 17 3 3 0 0 0 0 0 0 3 0 - 12963615 Tubb3 tubulin beta-3 chain 0 0 0 0 0,9905 26,2 14 4 0,9869 26,2 13 2 0,9884 29,6 14 6 - 100818178 KDM2A lysine-specific demethylase 2A 0 0 0 0 0 0 0 0 1 4,5 4 20 1 5,3 6 22 0,000137 calcium-binding tyrosine phosphorylation-regulated 109255218 Cabyr protein isoform c 0 0 0 0 0 0 0 0 1 31 10 63 0,9997 31 10 2 0,0000472 115430101 C4bp C4b-binding protein precursor 0 0 0 0 0,9793 3 1 1 1 6,8 3 4 1 6,8 3 5 0,0000947 117606270 NME2 nucleoside diphosphate kinase B 0,9794 7,6 1 1 0 0 0 0 1 67,1 8 8 1 67,1 8 11 0,000506 118130874 1700001P01Rikuncharacterized protein C17orf98 homolog 0,9562 4,5 1 1 0,9898 4,5 1 2 1 39,4 4 6 1 39,4 4 9 0,000426 118601013 Aprt adenine phosphoribosyltransferase 0 0 0 0 0,9879 5,6 1 1 1 23,3 3 4 1 23,3 3 5 0,000161 128485805 pbp2 phosphatidylethanolamine-binding protein 2 0 0 0 0 0,9895 10,7 1 1 1 31 4 3 1 31 4 4 0,000203 12963609 Qsox1 sulfhydryl oxidase 1 isoform b precursor 0 0 0 0 0,9731 1,9 1 1 1 8,3 4 6 1 9,7 5 9 0,000115

13386054 ARPC4 actin-related protein 2/3 complex subunit 4 isoform 1 0 0 0 0 0 0 0 0 1 16,1 3 3 1 16,1 3 3 0,000127 NADH dehydrogenase [ubiquinone] flavoprotein 3, 140969946 NDUFV3 mitochondrial isoform 2 0,984 10,6 1 1 0,9867 10,6 1 1 1 30,8 2 3 1 30,8 2 5 0,000269 149261044 Gm5428 PREDICTED: 60S ribosomal protein L6-like 0,9661 3,4 1 1 0,976 3,4 1 1 1 9,1 3 4 1 9,1 3 6 0,000118 15011841 gpX3 glutathione peroxidase 3 precursor 0 0 0 0 0,9588 11,5 2 1 1 21,7 4 3 1 21,7 4 4 0,000138 158262037 2310061I04Rik uncharacterized protein LOC69662 0 0 0 0 0 0 0 0 1 10,2 3 3 1 10,2 3 3 0,0000993 188219582 LOC231914 glucosamine-6-phosphate isomerase 1 0 0 0 0 0,9761 7,3 2 1 1 19,7 4 5 1 19,7 4 6 0,000124 19263322 WDR77 methylosome protein 50 0,97 4,4 1 1 0,9828 4,4 1 1 1 13,5 3 3 1 13,5 3 6 0,000199 19526481 Spna1 spectrin alpha chain, erythrocytic 1 0 0 0 0 0 0 0 0 1 1,7 4 4 1 1,7 4 4 0,0000107 198278549 4930443G12Riksolute carrier family 25, member 54 0 0 0 0 0,9809 3,4 1 1 1 8,9 3 3 1 8,9 3 4 0,000054 mitochondrial import inner membrane translocase 21313128 1700034H14Riksubunit Tim21 0 0 0 0 0 0 0 0 1 12,3 3 3 1 12,3 3 3 0,000065 sodium/potassium-transporting ATPase subunit alpha-1 21450277 ATP1A1 precursor 0 0 0 0 0 0 0 0 1 13,8 11 3 1 14,7 12 4 0,0000315 delta-1-pyrroline-5-carboxylate dehydrogenase, 225543103 ALDH4A1 mitochondrial precursor 0 0 0 0 0,9728 2,7 1 1 1 13,5 6 6 1 13,5 6 7 0,0000999 225543157 lpp lipoma-preferred partner homolog isoform 1 0 0 0 0 0 0 0 0 1 11,3 3 3 1 11,3 3 3 0,0000576 isocitrate dehydrogenase [NADP], mitochondrial 225579033 IDH2 precursor 0 0 0 0 0,9609 3,5 1 1 1 19 7 7 1 19 7 7 0,0000977 225735569 ALDH1A2 retinal dehydrogenase 2 0 0 0 0 0,9874 2,7 1 1 1 8,5 3 3 1 8,5 3 4 0,0000592 cytochrome c oxidase subunit II [Mus musculus 226453478 COX2 castaneus] 0 0 0 0 0 0 0 0 1 40,5 5 31 1 40,5 5 81 0,007415808 227330633 pgm2 phosphoglucomutase-2 0,9775 1,4 1 1 0,9898 2,3 1 1 1 10 5 5 1 10 5 8 0,0000863 very long-chain specific acyl-CoA dehydrogenase, 23956084 ACADVL mitochondrial precursor 0,9833 1,7 1 1 0 0 0 0 1 8,4 4 4 1 8,4 4 5 0,0000478 239916005 THOP1 thimet oligopeptidase 0 0 0 0 0 0 0 0 1 13,8 8 8 1 13,8 8 8 0,0000813 240120058 4930544G11Rikras homolog gene family, member A-like 0 0 0 0 0 0 0 0 1 26,4 4 10 1 26,4 4 17 0,000717 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 25092662 vps25 vacuolar protein-sorting-associated protein 25 0,979 5,7 1 2 0,9835 8,5 1 2 1 18,8 3 3 1 18,8 3 7 0,00027

254587998 4930432K09Rikuncharacterized protein LOC73779 isoform 2 precursor 0 0 0 0 0,9838 3,8 1 2 1 15,1 3 5 1 15,1 3 7 0,000432 254588110 Hist1h1d histone H1.3 0 0 0 0 0 0 0 0 1 15,1 3 3 1 15,1 3 3 0,000121 255069795 vars valine--tRNA ligase 0 0 0 0 0,9685 1,2 1 1 1 3,4 4 4 1 4,6 5 6 0,0000355 257196215 Ccdc116 coiled-coil domain-containing protein 116 0,9691 1,5 1 2 0,9813 1,5 1 5 1 5,2 2 10 1 5,2 2 17 0,000358 translocon-associated protein subunit delta isoform 1 262050625 Ssr4 precursor 0 0 0 0 0,9878 6,4 1 2 1 14 2 3 1 14 2 5 0,000293 262359885 eif5a eukaryotic translation initiation factor 5A-1 0 0 0 0 0,9845 7,8 1 1 1 28,6 3 4 1 28,6 3 5 0,000278

27229278 AGPAT3 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma 0 0 0 0 0 0 0 0 1 8 2 3 1 8 2 4 0,0000933 27229283 Rexo2 oligoribonuclease, mitochondrial precursor 0,9809 4,2 1 1 0,9879 4,2 1 1 1 17,3 3 3 1 17,3 3 5 0,000131 27501448 eIF6 eukaryotic translation initiation factor 6 0,9613 4,1 1 1 0 0 0 0 1 26,5 4 4 1 26,5 4 5 0,000211 ATP-binding cassette sub-family B member 8, 27753995 abcb8 mitochondrial precursor 0 0 0 0 0 0 0 0 1 6,4 3 3 1 6,4 3 3 0,0000313 309265504 Rsph10a PREDICTED: radial spoke head 10 homolog B 0 0 0 0 0 0 0 0 1 4,6 3 4 1 5,5 4 6 0,0000579 31543349 Nsf vesicle-fusing ATPase 0,9677 1,3 1 1 0 0 0 0 1 7,4 5 5 1 8,7 6 6 0,0000492 31559918 NRD1 nardilysin precursor 0 0 0 0 0 0 0 0 1 2,5 3 3 1 2,5 3 3 0,0000229 sterol-4-alpha-carboxylate 3-dehydrogenase, 31982437 nsdhl decarboxylating 0,9607 2,8 1 2 0,9791 2,8 1 1 1 10,5 3 3 1 10,5 3 6 0,000156 33504483 rps9 40S ribosomal protein S9 0 0 0 0 0 0 0 0 1 12,9 3 3 1 12,9 3 3 0,000083 dolichyl-diphosphooligosaccharide--protein 34996495 Rpn2 glycosyltransferase subunit 2 precursor 0 0 0 0 0 0 0 0 1 8,1 3 3 1 8,1 3 3 0,0000501 PREDICTED: axonemal dynein light chain domain- 377833680 Axdnd1 containing protein 1 0 0 0 0 0,9899 1,2 1 1 1 3,5 3 3 1 3,5 3 4 0,0000287 56961629 SPATA24 spermatogenesis-associated protein 24 isoform 1 0 0 0 0 0 0 0 0 1 20 3 3 1 20 3 4 0,000155

58743353 RNASE13 probable inactive ribonuclease-like protein 13 precursor 0 0 0 0 0 0 0 0 1 31,4 4 4 1 31,4 4 4 0,000269 6678189 Svs5 seminal vesicle secretory protein 5 precursor 0 0 0 0 0 0 0 0 1 47,5 3 4 1 47,5 3 4 0,000382 6679587 Rab1 ras-related protein Rab-1A 0,9688 5,9 1 1 0,9674 5,9 1 1 1 23,9 4 5 1 23,9 4 8 0,000207 6679891 GANAB neutral alpha-glucosidase AB 0 0 0 0 0 0 0 0 1 7,6 5 5 1 7,6 5 5 0,000048 6680121 Gm6665 glutathione S-transferase Mu 2 0 0 0 0 0 0 0 0 1 39,4 8 7 1 39,4 8 8 0,000189 6680836 CALR calreticulin precursor 0 0 0 0 0,9813 2,9 1 1 1 11,8 3 3 1 13,9 4 5 0,000103 6680988 Gm7795 cytochrome c oxidase subunit 6A1, mitochondrial 0,9807 14,3 1 1 0,9802 14,3 1 1 1 16,1 2 3 0,9997 14,3 1 4 0,000576 6681069 CSRP1 cysteine and glycine-rich protein 1 0 0 0 0 0,986 7,8 1 1 1 21,8 3 5 1 21,8 3 6 0,000169 6753490 COPS4 COP9 signalosome complex subunit 4 0,9684 3 1 1 0,9882 4,2 1 1 1 15,5 4 4 1 18,5 5 6 0,0000997 6753550 Acot1 acyl-coenzyme A thioesterase 1 0 0 0 0 0 0 0 0 1 10,3 4 4 1 17,9 7 11 0,000202 6754084 Gm5562 glutathione S-transferase Mu 1 0,9831 5,5 1 1 0,9701 17 3 1 1 28 5 4 1 28 5 6 0,000148 6996913 LOC100048867annexin A2 0 0 0 0 0,9847 4,7 1 1 1 13,6 4 4 1 13,6 4 5 0,0000823 70608131 IMMT mitochondrial inner membrane protein isoform 1 0 0 0 0 0 0 0 0 1 57,6 37 186 1 64,7 42 420 0,003471768 7305339 Odf2 outer dense fiber protein 2 isoform c 0 0 0 0 0,9877 51,1 32 4 1 50 31 284 0,9854 51,1 32 8 - 74024924 SUOX sulfite oxidase, mitochondrial precursor 0 0 0 0 0,9762 1,8 1 1 1 5,7 3 3 1 5,7 3 4 0,0000716 84490394 Spnb1 spectrin beta chain, erythrocytic 0 0 0 0 0 0 0 0 1 1,8 3 4 1 1,8 3 4 0,0000111 85861182 aldh1a1 retinal dehydrogenase 1 0 0 0 0 0 0 0 0 1 8,6 3 3 1 8,6 3 3 0,0000436 86198316 Pdia4 protein disulfide-isomerase A4 precursor 0 0 0 0 0 0 0 0 1 10,1 6 6 1 10,1 6 6 0,0000614 88196769 MLF1 myeloid leukemia factor 1 isoform b 0 0 0 0 0 0 0 0 1 31,5 7 10 1 42,3 8 26 0,000753 9790219 DSTN destrin 0 0 0 0 0,9878 6,7 1 1 1 41,8 5 7 1 48,5 6 9 0,000234 114145561 Gm5604 keratin, type II cytoskeletal 8 0 0 0 0 0 0 0 0 0,9999 6,3 3 4 0,9998 6,3 3 3 0,0000403 120407060 Antxrl anthrax toxin receptor-like precursor 0 0 0 0 0,9861 1,9 1 1 0,9998 3,7 2 3 0,9998 3,7 2 4 0,0000616 14149635 FABP4 fatty acid-binding protein, adipocyte 0 0 0 0 0 0 0 0 0,9998 18,9 2 2 0,9998 18,9 2 2 0,0000668 147905740 MUC5B mucin 5, subtype B, tracheobronchial precursor 0 0 0 0 0 0 0 0 0,9998 0,6 2 2 0,9998 0,6 2 2 0,0000057

149260287 LOC100045999PREDICTED: GTP-binding nuclear protein Ran-like 0 0 0 0 0,9669 25 5 1 0,9998 25 5 5 0,9999 36,1 7 8 0,000248 22296880 Fam3b protein FAM3B precursor 0,9581 3,8 1 1 0,9869 5,5 1 1 0,9998 10,6 2 2 1 14,5 3 5 0,000131 PREDICTED: sterile alpha motif domain-containing 309262734 Samd15 protein 15 0 0 0 0 0,9843 1,9 1 2 0,9998 5,2 2 3 0,9998 5,2 2 5 0,0000578 31560433 snx3 sorting nexin-3 0 0 0 0 0 0 0 0 0,9998 13,6 2 2 0,9998 13,6 2 2 0,0000696 31982030 ARHGDIA rho GDP-dissociation inhibitor 1 0,9854 7,8 1 1 0,9583 7,4 1 1 0,9998 15,2 2 3 0,9998 15,2 2 5 0,000193 58037267 Pdia6 protein disulfide-isomerase A6 precursor 0,9873 11,5 4 2 0,9899 9,9 3 1 0,9998 21,3 7 8 0,9882 21,3 7 4 - 90017457 FDX1L adrenodoxin-like protein, mitochondrial precursor 0 0 0 0 0,9899 5,7 1 2 0,9998 13,2 2 2 0,9999 13,2 2 4 0,00015 12963799 ATP6V1D V-type proton ATPase subunit D 0,9648 4,5 1 1 0,9768 6,5 1 1 0,9997 11,3 2 2 1 15,8 3 4 0,000102 168693635 nagA alpha-N-acetylgalactosaminidase precursor 0,9618 2,7 1 2 0,9898 3,4 1 1 0,9997 5,5 2 3 1 8,2 3 7 0,000116 170650609 INPP1 inositol polyphosphate 1-phosphatase 0 0 0 0 0,9891 3 1 1 0,9997 7,1 2 2 0,9998 7,1 2 3 0,0000638 21703344 sar1a GTP-binding protein SAR1a 0 0 0 0 0 0 0 0 0,9997 11,6 2 2 0,9918 11,6 2 3 0,00011 227497595 Adcy10 adenylate cyclase type 10 0 0 0 0 0,9798 0,7 1 1 0,9997 1,4 2 2 0,9997 1,4 2 3 0,0000154 low molecular weight phosphotyrosine protein 31542070 acp1 phosphatase isoform 2 0 0 0 0 0 0 0 0 0,9997 18,4 2 2 1 31,6 4 5 0,000187 31543255 MME neprilysin 0 0 0 0 0 0 0 0 0,9997 3,5 2 2 1 4,7 3 3 0,0000304 31543976 YWHAG 14-3-3 protein gamma 0,9862 9,7 2 1 0,9833 12,6 3 1 0,9997 13,8 3 2 0,9997 13,8 3 4 0,0000969 peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine 31981178 ngly1 amidase 0,9828 1,8 1 1 0 0 0 0 0,9997 3,5 2 2 0,9998 3,5 2 3 0,0000284 PREDICTED: transmembrane emp24 domain- 407263560 Tmed9 containing protein 2-like 0,9665 4,5 1 2 0,9819 6,5 1 1 0,9997 10,9 2 2 0,9998 10,9 2 5 0,000202 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX thioredoxin-dependent peroxide reductase, 6680690 prdx3 mitochondrial precursor 0 0 0 0 0 0 0 0 0,9997 10,1 2 2 1 14,4 3 3 0,0000972 110347473 Apoa4 apolipoprotein A-IV precursor 0 0 0 0 0 0 0 0 0,9996 6,6 2 2 0,9997 6,6 2 2 0,0000361 mesencephalic astrocyte-derived neurotrophic factor 110625813 manf precursor 0 0 0 0 0,9853 7,8 1 1 0,9996 13,4 2 2 1 14 3 4 0,000112 116812642 Sbpl spermine binding protein like precursor 0 0 0 0 0 0 0 0 0,9996 11,6 2 3 0,9997 11,6 2 3 0,0000905 13277350 Wdr61 WD repeat-containing protein 61 isoform a 0 0 0 0 0 0 0 0 0,9996 12,1 2 2 0,9997 12,1 2 2 0,0000549 dolichyl-diphosphooligosaccharide--protein 164518942 DAD1 glycosyltransferase subunit DAD1 0,9706 8,8 1 1 0 0 0 0 0,9996 19,5 2 2 0,9997 19,5 2 3 0,000255 255522841 BC051142 testis specific basic protein isoform 2 0 0 0 0 0,9861 7 1 1 0,9996 12,6 2 2 0,9995 12,6 2 3 0,00015 257467625 FCGBP Fc fragment of IgG binding protein-like precursor 0 0 0 0 0 0 0 0 0,9996 0,9 2 2 0,9997 0,9 2 6 0,0000249 31560378 Tspan6 tetraspanin-6 0,9569 4,9 1 1 0,9889 4,9 1 1 0,9996 8,2 2 2 0,9997 8,2 2 4 0,000148 long-chain specific acyl-CoA dehydrogenase, 31982520 acadl mitochondrial precursor 0,9824 3 1 1 0,9873 2,6 1 1 0,9996 5,6 2 2 1 8,6 3 4 0,000063 34850748 MORN2 MORN repeat-containing protein 2 0,9751 11,4 1 1 0,9898 11,4 1 1 0,9996 32,9 2 3 0,9997 32,9 2 5 0,000402 58037191 RAB28 ras-related protein Rab-28 0,9798 5,9 1 1 0,982 8,1 1 1 0,9996 14 2 2 0,9998 14 2 4 0,0000993 6678099 SERPINE2 glia-derived nexin precursor 0 0 0 0 0 0 0 0 0,9996 4,8 2 2 0,9996 4,8 2 2 0,0000402 6680019 GCLM glutamate--cysteine ligase regulatory subunit 0,9795 5,5 1 1 0,9755 5,5 1 1 0,9996 10,2 2 2 0,9997 10,2 2 4 0,000178 cell division control protein 42 homolog isoform 1 6753364 Cdc42 precursor 0 0 0 0 0 0 0 0 0,9996 12 2 2 1 17,8 3 3 0,000106 114205406 Mcpt3 mast cell protease 4 precursor 0,9665 4,5 1 1 0 0 0 0 0,9995 9,8 2 2 0,9996 9,8 2 3 0,000111 125660458 SLC30A9 zinc transporter 9 0 0 0 0 0 0 0 0 0,9995 3,7 2 2 1 4,9 3 3 0,0000415 140970996 1700013B16Rikuncharacterized protein LOC72219 0 0 0 0 0,9821 1 1 1 0,9995 2,4 2 2 0,9996 2,4 2 3 0,0000252 157951741 cat catalase 0 0 0 0 0 0 0 0 0,9995 6,1 2 2 0,9996 6,1 2 2 0,0000265

170650630 Sirt2 NAD-dependent protein deacetylase sirtuin-2 isoform 1 0 0 0 0 0 0 0 0 0,9995 6,8 2 2 0,9996 6,8 2 2 0,0000405 188035926 NDUFAF2 mimitin, mitochondrial 0 0 0 0 0 0 0 0 0,9995 13,7 2 2 0,9995 13,7 2 3 0,000088 225690589 htatip2 oxidoreductase HTATIP2 isoform a precursor 0 0 0 0 0 0 0 0 0,9995 8,7 2 2 0,9995 8,7 2 2 0,0000526 protein disulfide-isomerase-like protein of the testis 253735751 Pdilt precursor 0 0 0 0 0,9819 2 1 1 0,9995 4,9 2 2 0,9996 4,9 2 3 0,000035 262231811 Dynlt1b dynein light chain Tctex-type 1F isoform 1 0,9671 15,1 1 1 0,9898 15,1 1 1 0,9995 15,1 1 2 0,9997 15,1 1 4 0,000342 28077019 1700040L02Rikuncharacterized protein C10orf107 homolog 0 0 0 0 0,9722 3,6 1 1 0,9995 6,8 2 2 0,9996 6,8 2 3 0,0000874 293651573 - predicted gene 6760 0 0 0 0 0 0 0 0 0,9995 25,3 2 2 0,9995 25,3 2 2 0,0000988 357527382 Maai maleylacetoacetate isomerase isoform 2 0 0 0 0 0 0 0 0 0,9995 15,5 2 2 0,9996 15,5 2 2 0,0000829

377833075 LOC380654 PREDICTED: uncharacterized protein LOC380654 0 0 0 0 0,985 0,5 1 1 0,9995 0,9 2 2 0,9995 0,9 2 3 0,00000908 40789280 FAF1 FAS-associated factor 1 0,9725 1,7 1 1 0 0 0 0 0,9995 3,1 2 2 1 4,8 3 3 0,0000409 6755358 rpl8 60S ribosomal protein L8 0,9767 4,3 1 3 0,9837 6,2 1 1 0,9995 10,5 2 3 0,9996 10,5 2 8 0,000247 113931154 1600016N20Rikuncharacterized protein C11orf35 homolog 0 0 0 0 0,9884 2,3 1 1 0,9994 3,9 2 2 0,9995 3,9 2 3 0,0000372 13385270 HINT3 histidine triad nucleotide-binding protein 3 0 0 0 0 0 0 0 0 0,9994 15,2 2 2 0,9995 15,2 2 2 0,000101 148747526 Rab7 ras-related protein Rab-7a 0,9862 6,8 1 1 0 0 0 0 0,9994 12,1 2 2 0,9996 12,1 2 3 0,0000646 149363634 ptrh2 peptidyl-tRNA hydrolase 2, mitochondrial isoform b 0 0 0 0 0,9707 8,3 1 1 0,9994 16 2 2 0,9995 16 2 3 0,000111 153792526 arl1 ADP-ribosylation factor-like protein 1 0 0 0 0 0 0 0 0 0,9994 11 2 2 0,9994 11 2 3 0,00011 1-acyl-sn-glycerol-3-phosphate acyltransferase beta 23956162 AGPAT2 precursor 0,9795 3,2 1 1 0 0 0 0 0,9994 6,8 2 2 0,9994 6,8 2 3 0,0000818 254675147 1700011E24Rikuncharacterized protein C2orf61 homolog 0,9863 5,7 1 1 0,9818 4,5 1 1 0,9994 10,1 2 2 0,9998 10,1 2 4 0,000101 283945572 H2AFY core histone macro-H2A.1 isoform 2 0 0 0 0 0 0 0 0 0,9994 5,2 2 2 0,9994 5,2 2 2 0,0000408 31981304 atp6v0d1 V-type proton ATPase subunit d 1 0 0 0 0 0 0 0 0 0,9994 6,6 2 2 0,9994 6,6 2 2 0,0000417 6678760 Lypla1 acyl-protein thioesterase 1 0,9864 5,7 1 1 0,9825 4,8 1 1 0,9994 10,4 2 2 0,9998 10,4 2 4 0,000166 125656150 hsdl2 hydroxysteroid dehydrogenase-like protein 2 0 0 0 0 0 0 0 0 0,9993 4,9 2 2 0,9994 4,9 2 2 0,0000288 13385084 NIPSNAP3B protein NipSnap homolog 3B 0 0 0 0 0 0 0 0 0,9993 11,7 3 4 0,9994 11,7 3 4 0,000131 13591860 tollip toll-interacting protein 0,9856 5,1 1 1 0,9882 5,1 1 1 0,9993 9,9 2 2 0,9994 9,9 2 4 0,000117 ATP-binding cassette, sub-family A (ABC1), member 157057142 Abca14 14 0,9817 0,8 1 1 0,9879 0,7 1 1 0,9993 1,4 2 2 1 2,2 3 4 0,0000213 21312532 tmem38b trimeric intracellular cation channel type B 0 0 0 0 0,9804 5,1 1 1 0,9993 8,6 2 2 0,9995 8,6 2 4 0,000122 PREDICTED: cytochrome c oxidase subunit 7C, 407261032 Cox7c mitochondrial-like 0,9811 14,3 1 1 0,9633 14,3 1 1 0,9993 28,6 2 2 0,9994 28,6 2 4 0,000447 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, 7949037 ech1 mitochondrial precursor 0 0 0 0 0,9672 4,9 1 1 0,9993 7,6 2 3 0,9993 7,6 2 4 0,0000814 113930712 Mup14 major urinary protein 2 precursor 0 0 0 0 0 0 0 0 0,9992 13,3 2 2 0,9993 13,3 2 2 0,0000629 116517334 fhl1 four and a half LIM domains protein 1 isoform 3 0 0 0 0 0 0 0 0 0,9992 9,6 2 2 0,9994 9,6 2 2 0,0000391 162287063 Cnnm4 metal transporter CNNM4 precursor 0 0 0 0 0,9862 1,4 1 1 0,9992 3,2 2 2 0,9995 3,2 2 3 0,0000314 485050188 - predicted gene 11780 0 0 0 0 0 0 0 0 0,9992 9,9 2 2 0,9993 9,9 2 2 0,0000833 8393988 PMM2 phosphomannomutase 2 0 0 0 0 0,9811 4,5 1 1 0,9992 7,9 2 2 0,9994 7,9 2 3 0,0000607 112293266 HSPA4 heat shock 70 kDa protein 4 0 0 0 0 0 0 0 0 0,9991 4,8 3 2 0,9992 4,8 3 2 0,0000157 29789263 trim23 E3 ubiquitin-protein ligase TRIM23 0 0 0 0 0 0 0 0 0,9991 3,6 2 2 0,9991 3,6 2 2 0,0000279 226437676 NUP205 nucleoporin 205 0 0 0 0 0 0 0 0 0,999 1,1 2 2 0,999 1,1 2 2 0,00000956 51921291 SLC13A5 solute carrier family 13 member 5 0,965 1,7 1 1 0,9755 1,4 1 1 0,999 3,7 2 2 1 5,4 3 4 0,000105 6671571 ARF2 ADP-ribosylation factor 2 0 0 0 0 0 0 0 0 0,999 53,6 7 5 0,999 53,6 7 8 0,000314 a disintegrin and metalloprotease domain 3 (cyritestin) 121949804 Adam3 precursor 0 0 0 0 0 0 0 0 0,9989 3,3 2 3 0,9991 3,3 2 5 0,0000553 6755206 psmb7 proteasome subunit beta type-7 precursor 0,9538 3,6 1 1 0,9801 3,6 1 1 0,9989 7,2 2 2 0,9993 7,2 2 4 0,000114 148747177 RAB22A ras-related protein Rab-22A 0 0 0 0 0 0 0 0 0,9988 13,9 2 2 0,9991 13,9 2 2 0,000057 Réplica 1 Réplica 2 Réplica 3 Datos combinados Probabilidad Porcentaje Números de Números de Números de Referencia Probabilidad de Porcentaje Números de Número de de de péptidos Número de Probabilidad de Porcentaje péptidos Número de Probabilidad de Porcentaje péptidos Número de GI Proteína Nombre de la proteína coincidencia de cobertura péptidos únicos espectros coincidencia cobertura únicos espectros coincidencia de cobertura únicos espectros coincidencia de cobertura únicos espectros Valor APEX 21450325 BLVRB flavin reductase (NADPH) 0 0 0 0 0,9815 8,3 1 1 0,9988 15,5 2 2 1 23,8 3 4 0,000142

9789933 NUDT3 diphosphoinositol polyphosphate phosphohydrolase 1 0 0 0 0 0,9853 8,3 1 1 0,9988 10,1 2 3 1 18,5 3 4 0,000172 110625948 Acat3 acetyl-Coenzyme A acetyltransferase 3 0,96 2,5 1 1 0 0 0 0 0,9987 5,3 2 2 1 5,3 2 3 0,0000662 124486624 4932425I24Rik protein MAATS1 0,9682 1,8 1 1 0,9681 2,6 1 1 0,9987 4,3 2 4 0,999 4,3 2 6 0,0000587 157266281 Trappc5 trafficking protein particle complex subunit 5 0,9785 5,3 1 1 0 0 0 0 0,9987 9,6 2 2 0,9991 9,6 2 3 0,0000944 158966704 LOC10004750140S ribosomal protein S16 0,9676 5,5 1 1 0,9571 6,8 1 1 0,9987 12,3 2 2 1 17,8 3 4 0,000135 13385268 CYB5 cytochrome b5 0 0 0 0 0,9693 9 1 1 0,9986 24,6 2 3 0,9991 24,6 2 4 0,000243 PREDICTED: ubiquitin-conjugating enzyme E2 L3- 149260690 LOC100045901like 0 0 0 0 0 0 0 0 0,9985 24 2 2 0,9989 24 2 2 0,00011 MOSC domain-containing protein 2, mitochondrial 19526848 MOSC2 precursor 0 0 0 0 0 0 0 0 0,9985 7,4 2 2 0,9986 7,4 2 2 0,0000385 20846986 RPS25 PREDICTED: 40S ribosomal protein S25-like 0 0 0 0 0 0 0 0 0,9985 15,2 2 2 0,9985 15,2 2 2 0,0000813 165932306 SOX5 transcription factor SOX-5 isoform a 0 0 0 0 0 0 0 0 0,9984 1,9 1 2 0,9984 1,9 1 2 0,0000237 188219614 Uchl1 ubiquitin carboxyl-terminal hydrolase isozyme L1 0 0 0 0 0,9568 3,6 1 1 0,9984 11,7 2 2 0,9999 15,2 3 3 0,000102 62000644 CAPN11 calpain-11 0 0 0 0 0,9732 1,1 1 1 0,9984 2,2 2 2 0,9999 4,3 3 5 0,0000512 113195690 Man2b1 lysosomal alpha-mannosidase precursor 0 0 0 0 0 0 0 0 0,9983 3,4 2 2 0,9985 3,4 2 2 0,0000199

31543918 ube2v2 ubiquitin-conjugating enzyme E2 variant 2 isoform 1 0 0 0 0 0 0 0 0 0,9982 13,1 2 2 0,9986 13,1 2 3 0,000113 340139110 Cav1 caveolin-1 isoform 2 0 0 0 0 0 0 0 0 0,9982 10,9 2 2 0,9999 25,2 3 3 0,00016 117935040 4933402N03Rikuncharacterized protein LOC233918 0 0 0 0 0 0 0 0 0,9978 7,3 2 3 0,9981 7,3 2 3 0,0000559 227496381 TASP1 threonine aspartase 1 isoform a 0,9554 2,1 1 1 0,9587 3,7 1 1 0,9978 4,2 2 2 1 9,9 4 4 0,0000834 31543974 YWHAB 14-3-3 protein beta/alpha 0 0 0 0 0 0 0 0 0,9978 17,5 3 2 0,9981 21,5 4 3 0,0000825 6755923 LOC100040390ubiquitin-like modifier-activating enzyme 1 Y 0 0 0 0 0 0 0 0 0,9945 2,9 3 4 0,9946 2,9 3 5 0,0000496 149247503 LOC100046536PREDICTED: glutathione S-transferase P 2-like 0 0 0 0 0 0 0 0 0,993 15,2 2 2 0,9938 15,2 2 3 0,000146 13385770 Ubl4b ubiquitin-like protein 4B 0,978 9,6 1 1 0,9897 9,6 1 1 0,9923 9,6 1 2 0,9893 9,6 1 4 - haloacid dehalogenase-like hydrolase domain- 21313310 Gm10784 containing protein 2 isoform 1 0 0 0 0 0 0 0 0 0,9923 24 1 1 0,9993 18,5 2 2 0,0000614 cytochrome c oxidase subunit I [Mus musculus 167716839 COX1 musculus] 0 0 0 0 0 0 0 0 0,9919 6,2 1 1 0,9888 6,2 1 1 - 10092608 LOC100046536glutathione S-transferase P 1 0,9862 7,6 1 1 0 0 0 0 0,9916 12,9 2 2 0,9922 12,9 2 3 0,00014 31542119 Agtrap type-1 angiotensin II receptor-associated protein 0 0 0 0 0 0 0 0 0,9916 24,8 1 1 0,9884 24,8 1 1 - Tabla suplementaria 9. Datos de MS para las proteínas identificadas en la especie Mus spicilegus.

Réplica 1 Réplica 2 Réplica 3 Datos combinados

110625719 HINT2 histidine triad nucleotide-binding protein 2, mitochondrial precursor 1 29,4 3 4 1 17,2 2 3 0,9998 17,2 2 2 1 29,4 3 9 0,000357 110625839 NME5 nucleoside diphosphate kinase homolog 5 1 13,3 2 3 1 35,5 6 6 1 36,5 6 6 1 40,3 7 15 0,000678 110625843 LRRC34 leucine-rich repeat-containing protein 34 1 8,7 3 3 - 0 0 0 1 17,6 7 7 1 23,6 9 11 0,000223 110625851 Tmprss12 transmembrane protease serine 12 precursor 1 7,1 2 5 0,9994 7,1 2 2 1 10,1 3 3 1 10,1 3 10 0,000307 110625871 TEKT4 tektin-4 1 25,7 9 10 - 0 0 0 1 40,5 16 24 1 44,5 18 34 0,000455 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial 110625954 Ndufv2 isoform 1 precursor 1 36,3 8 21 1 33,9 7 19 1 41,1 9 27 1 41,1 9 67 0,00189715 110625979 LOC100047986 elongation factor 1-gamma 1 8,9 3 4 0,9999 7,8 3 3 1 32 13 20 1 32 13 27 0,000552 110626034 4930519F16Rik uncharacterized protein LOC75106 precursor 1 21,2 3 14 1 23,4 4 8 1 35,5 5 22 1 35,5 5 43 0,00212238 110626050 them4 acyl-coenzyme A thioesterase THEM4 1 24,8 5 7 1 29,1 6 7 1 30,9 8 9 1 36,5 9 23 0,000867 110626054 SPACA3 sperm acrosome membrane-associated protein 3 precursor 1 20,9 3 4 1 20,9 3 5 1 20,9 3 4 1 20,9 3 13 0,00068 110626086 Arrdc5 arrestin domain-containing protein 5 1 18,5 6 6 1 12 5 5 1 19,1 6 6 1 25,8 9 18 0,000405 complement component 1 Q subcomponent-binding protein, 112181167 c1qbp mitochondrial 1 26,9 4 9 1 26,9 4 8 1 26,9 4 9 1 26,9 4 28 0,00103789 112181182 COX5A cytochrome c oxidase subunit 5A, mitochondrial precursor 1 48,6 7 26 1 48,6 7 14 1 48,6 7 18 1 53,4 8 60 0,00303603 112293264 PDIA3 protein disulfide-isomerase A3 precursor 1 20,2 9 9 1 7,7 3 6 1 32,5 14 16 1 36,2 16 31 0,000395 112734843 Tssk2 testis-specific serine/threonine-protein kinase 2 1 9,8 4 7 1 12,8 5 7 1 16,5 5 5 1 24 8 16 0,00029 112734851 Tssk1 testis-specific serine/threonine-protein kinase 1 1 20,5 7 12 0,9992 10,4 4 4 1 29,3 9 21 1 35,6 11 40 0,0007 112807195 Gm11273 cytochrome c oxidase subunit 5B, mitochondrial 1 46,5 5 15 1 47,3 6 19 1 47,3 6 15 1 62,8 7 48 0,00213906 113865881 Gm6685 cytochrome b5 domain-containing protein 1 1 9,6 3 3 0,9996 6,1 2 3 1 19,7 4 4 1 20,2 5 10 0,000392 113866007 Igbp1b immunoglobulin-binding protein 1b 1 12,5 4 9 0,9998 6,1 2 3 1 18,7 6 11 1 18,7 6 23 0,000592 113866024 rab5c ras-related protein Rab-5C 1 47,7 7 12 1 47,7 7 11 1 54,2 8 12 1 54,2 8 32 0,00107501 114145546 Dnaic2 dynein intermediate chain 2, axonemal 1 13,2 6 6 1 28,3 13 19 1 35,8 17 24 1 39,2 18 50 0,000724 114145561 Gm5604 keratin, type II cytoskeletal 8 1 14,9 7 16 1 5,9 3 3 0 0 0 0 1 16,5 8 18 0,000266 114326406 Acrv1 acrosomal protein SP-10 precursor 1 17,6 3 12 1 18 4 18 1 17,6 3 32 1 18 4 63 0,00565524 114520613 1110017D15Rik spermatid-specific manchette-related protein 1 isoform 1 1 13,8 3 5 - 54,2 13 1 0 0 0 0 0,9734 54,2 13 1 - disintegrin and metalloproteinase domain-containing protein 1b 114842412 Adam1b precursor 1 9,3 6 10 1 6,8 4 4 1 7,1 5 5 1 9,3 6 19 0,000275 11496255 LOC100048331 dnaJ homolog subfamily A member 4 1 11,6 4 5 - 0 0 0 1 16,9 5 5 1 17,1 6 9 0,000142 115334671 ALDH9A1 4-trimethylaminobutyraldehyde dehydrogenase precursor 1 8,1 4 4 - 0 0 0 1 25,5 10 13 1 27,6 11 17 0,000245 116014342 bsg basigin isoform 2 precursor 1 17,6 5 33 1 17,9 6 47 1 17,9 6 38 1 17,9 6 125 0,00415427 116063531 CAR1 carbonic anhydrase 1 1 10,7 3 3 1 13,4 2 3 1 22,2 4 5 1 25,7 5 11 0,000408 116734870 LTA4H leukotriene A-4 hydrolase 1 13,4 6 7 - 0 0 0 0,986 2 1 1 1 13,4 6 8 0,000107 116805338 3110056O03Rik signal peptide peptidase-like 2B precursor 1 8 3 5 0,9995 5,7 2 3 1 10,6 4 4 1 10,6 4 13 0,000336 117606270 NME2 nucleoside diphosphate kinase B 1 13,8 2 5 - 0 0 0 0,9999 50 6 6 0,9999 50 6 8 0,000423 117606409 wbp2nl postacrosomal sheath WW domain-binding protein 1 8,6 3 10 1 14,8 4 9 1 13,6 4 12 1 17,3 5 31 0,00107748 117935034 1700061G19Rik uncharacterized protein LOC78625 1 5,7 4 6 1 11,2 6 8 1 7,7 5 8 1 14,2 8 22 0,000275 117956379 tekt1 tektin-1 1 32,8 11 21 1 10,3 4 6 1 23,9 10 16 1 36,4 14 42 0,000657 118150641 ropn1l ropporin-1-like protein 1 28 5 8 1 32,6 7 8 1 36,2 9 17 1 36,2 9 33 0,000982 succinyl-CoA:3-ketoacid coenzyme A transferase 2A, 11968160 Oxct2a mitochondrial precursor 1 60,2 21 155 1 72,5 25 255 1 74,2 29 515 1 74,4 29 926 0,00901552 119709839 Adam6a a disintegrin and metalloprotease domain 6 1 16,2 10 21 1 16,4 10 14 1 16,4 10 19 1 22 13 56 0,000675 120407035 Spam1 hyaluronidase PH-20 precursor 1 24,6 11 15 1 11,9 4 5 1 24,2 12 18 1 31,1 15 40 0,000642 120587011 SYNGR4 synaptogyrin-4 1 15 3 19 1 15 3 19 1 15 3 23 1 15 3 61 0,00441607 121674797 PPT1 palmitoyl-protein thioesterase 1 precursor 1 10,8 2 4 1 16,7 4 5 1 13,7 3 5 1 16,7 4 14 0,00045

121949804 Adam3 a disintegrin and metalloprotease domain 3 (cyritestin) precursor 1 7,1 4 5 - 0 0 0 1 7,1 4 4 1 7,1 4 10 0,000119 12331398 SCAMP2 secretory carrier-associated membrane protein 2 1 9,7 2 9 1 9,7 2 12 1 9,7 2 6 1 9,7 2 27 0,00242825 124001554 CATSPER2 cation channel sperm-associated protein 2 1 7,3 4 4 1 4,4 2 3 1 6,1 3 3 1 9,9 5 10 0,000214 124244035 PPEF1 serine/threonine-protein phosphatase with EF-hands 1 1 11,5 6 8 1 17,5 9 16 1 24,6 12 18 1 28,9 14 41 0,000548 124248577 psmc4 26S protease regulatory subunit 6B 1 13,4 5 5 - 0 0 0 1 10,5 4 4 1 19,6 7 9 0,0002 124301188 4732415M23Rik uncharacterized protein C16orf55 homolog 1 37,9 4 7 0,9981 13,6 2 2 1 37,9 4 6 1 37,9 4 15 0,000865 Réplica 1 Réplica 2 Réplica 3 Datos combinados

12963571 Ndufa7 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 1 38,9 4 5 1 39,8 7 12 1 39,8 6 7 1 39,8 7 24 0,00104827 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12963633 Ndufa13 13 1 48,6 7 23 1 48,6 7 12 1 47,9 6 18 1 48,6 7 54 0,00236615 12963687 SSNA1 Sjoegren syndrome nuclear autoantigen 1 homolog 1 21 3 3 0,9997 24,4 2 2 1 33,6 3 3 1 34,5 4 8 0,000431 131889222 SDR39U1 epimerase family protein SDR39U1 1 16,4 5 11 1 15 5 7 1 19,1 5 9 1 19,5 6 28 0,000853 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13195624 NDUFA10 10, mitochondrial precursor 1 29 9 12 1 37,5 13 31 1 43,1 13 32 1 52,1 17 76 0,00166784 13195690 Gm5928 ubiquitin-40S ribosomal protein S27a precursor 1 44,6 3 27 1 56,4 4 21 1 44,6 3 34 1 56,4 4 83 0,00339408 13249349 RANBP17 ran-binding protein 17 1 8,8 9 12 1 20,6 18 26 1 19,9 17 20 1 27,4 23 59 0,000508 CUB and zona pellucida-like domain-containing protein 1 133778951 CUZD1 precursor 1 7,6 4 5 1 13,3 5 11 1 14,3 6 10 1 20,8 8 26 0,000519 13384638 Rsph1 radial spoke head 1 homolog 1 20,9 5 8 1 13,3 3 6 1 28,6 6 18 1 28,6 6 31 0,00110279 13384778 pgls 6-phosphogluconolactonase 1 30,7 6 6 1 20,2 4 4 1 21,4 4 4 1 41,2 8 15 0,000483 13384810 ACYP1 acylphosphatase-1 1 48,5 4 4 0,9996 27,3 2 2 0,9997 27,3 2 2 1 48,5 4 8 0,000466 13385006 cyc1 cytochrome c1, heme protein, mitochondrial 1 40 8 24 1 35,4 7 33 1 42,5 9 41 1 45,2 10 99 0,00372824 13385084 NIPSNAP3B protein NipSnap homolog 3B 1 37,7 6 6 1 23,1 4 4 1 27,9 5 7 1 37,7 6 17 0,000619 13385124 ribC1 RIB43A-like with coiled-coils protein 1 1 14,2 4 7 - 0 0 0 1 14,2 4 9 1 14,2 4 17 0,000278 13385168 uqcrfs1 cytochrome b-c1 complex subunit Rieske, mitochondrial 1 42,7 9 36 1 47,8 11 34 1 48,2 11 48 1 53,3 13 118 0,00371804 13385180 Spesp1 sperm equatorial segment protein 1 precursor 1 33,6 10 95 1 36,8 11 78 1 37,1 12 126 1 42,9 14 302 0,00731015 13385188 KLHL10 kelch-like protein 10 1 13,2 7 7 0,9991 3,6 2 2 1 12,8 7 7 1 16,1 9 17 0,000203 13385274 LOC100045163 phospholipase B-like 1 precursor 1 16,7 7 11 1 9,3 4 4 1 22,4 9 14 1 24,4 10 29 0,000509

13385322 Ndufb7 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 1 46,7 5 13 1 46,7 5 6 1 70,8 8 18 1 70,8 8 37 0,00157041

13385492 ndufa6 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 1 37,4 4 7 1 39,7 4 7 1 45,8 5 9 1 45,8 5 24 0,00124773 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, 13385558 Ndufb8 mitochondrial precursor 1 46,2 6 12 0,9994 10,8 2 2 1 46,2 6 18 1 46,2 6 33 0,00120664 13385726 Gm2962 cytochrome b-c1 complex subunit 7 1 27,9 4 7 1 28,8 5 9 1 28,8 5 11 1 28,8 5 27 0,00125756 13385730 GLIPR1L2 GLIPR1-like protein 2 1 24,1 6 23 1 9,3 3 5 1 15,7 4 11 1 24,1 6 39 0,00141888 13385762 Lrrc18 leucine-rich repeat-containing protein 18 isoform 1 1 16,1 3 7 1 19,6 4 4 1 22 4 11 1 33,3 6 21 0,000495 13385942 CS citrate synthase, mitochondrial precursor 1 30,6 12 18 1 28,7 11 21 1 42,2 14 127 1 42,2 14 244 0,00434743

13386100 NDUFA5 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 1 45,7 6 9 1 60,3 6 8 1 60,3 7 10 1 60,3 7 27 0,0016118 134031980 Irgc1 interferon-inducible GTPase 5 1 26,8 9 10 1 22,5 9 24 1 41,9 17 59 1 41,9 17 93 0,00143537 134031994 psmA2 proteasome subunit alpha type-2 1 28,6 5 6 1 29,1 6 7 1 38 7 13 1 38 7 25 0,00105191 134053905 Psmd11 26S proteasome non-ATPase regulatory subunit 11 1 24,9 9 9 - 0 0 0 1 25,8 9 9 1 29,6 10 17 0,000269 134053952 wdr16 WD repeat-containing protein 16 1 19 12 14 1 24,8 14 21 1 45,5 23 59 1 45,5 23 95 0,00116922 134053957 SLC44A5 choline transporter-like protein 5 1 12,8 7 12 1 15,8 8 19 1 16,3 9 16 1 19,3 10 47 0,000775 13507710 Ropn1 ropporin-1 1 53,3 8 41 1 49,1 7 24 1 64,2 9 91 1 73,1 10 152 0,00415039 139948802 Uchl3 ubiquitin carboxyl-terminal hydrolase isozyme L3 1 27,8 4 5 1 31,7 6 8 1 43 7 11 1 47,4 8 24 0,000863 145301549 Hba-a1 hemoglobin alpha, adult chain 2 1 41,5 5 40 1 61,3 6 21 1 35,2 4 9 1 61,3 6 72 0,00386816

145587094 9430016H08Rik uncharacterized protein C2orf47 homolog, mitochondrial precursor 1 18,2 5 5 1 11,3 3 4 1 10,3 3 4 1 21 6 13 0,000325 145966883 PSMD14 26S proteasome non-ATPase regulatory subunit 14 1 14,8 3 3 0,9993 8,7 2 2 0,9996 6,5 2 2 1 19,4 4 7 0,000271 145966885 SPACA5 sperm acrosome-associated protein 5 precursor 1 13,1 3 4 1 25 3 5 1 25 4 5 1 25 4 14 0,000773 14780884 POLDIP2 polymerase delta-interacting protein 2 1 13,6 4 5 1 10,9 3 3 1 21,5 6 6 1 24,2 7 14 0,000289 148225693 TEKT2 tektin-2 1 45,1 16 21 1 21,2 7 14 1 40,7 14 31 1 46,7 17 66 0,000909

148230380 Pebp4 phosphatidylethanolamine-binding protein 4 isoform a precursor 1 19 5 9 1 12,4 3 3 1 16,1 4 10 1 19 5 22 0,000731 [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, 148277602 Pdp1 mitochondrial isoform b 1 10,8 5 5 - 0 0 0 0,9997 4,6 2 2 1 10,8 5 7 0,000111 148747546 Serpina3k serine protease inhibitor A3K precursor 1 10,8 3 4 - 0 0 0 0 0 0 0 1 10,8 3 4 0,0000963 Réplica 1 Réplica 2 Réplica 3 Datos combinados

149274885 LOC100046684 PREDICTED: ES1 protein homolog, mitochondrial-like isoform 1 1 16,2 3 4 1 26,7 5 7 1 41,7 7 9 1 44,7 8 21 0,000558 149944431 TEKT5 tektin-5 1 16,1 6 6 1 23,6 9 18 1 33,5 12 32 1 39,3 14 56 0,00094 152963553 1190002A17Rik adenylate kinase 8 1 26,5 11 17 1 11,9 5 6 1 27,6 12 22 1 33,2 16 46 0,000624 153791341 Rsph3b radial spoke head protein 3 homolog B 1 13,6 4 15 0,9996 5,1 2 4 1 16,7 5 16 1 16,7 5 35 0,000799 153791370 odf3 outer dense fiber protein 3 1 46,9 9 28 1 55,5 12 45 1 69,7 14 69 1 69,7 14 145 0,00383483 154091013 CCDC135 coiled-coil domain-containing protein lobo homolog 1 4 3 3 1 10,6 8 15 1 10 7 11 1 14,7 11 30 0,000277 154146209 Usp7 ubiquitin carboxyl-terminal hydrolase 7 1 2,2 3 3 1 16 16 20 1 19,2 17 19 1 24,6 23 42 0,000304 157057150 Abca17 ATP-binding cassette, sub-family A (ABC1), member 17 1 4,5 7 7 1 1,8 3 3 1 8,5 10 10 1 10,6 13 21 0,000143 157951596 Car2 carbonic anhydrase 2 1 26,2 4 4 1 46,2 7 11 1 53,1 10 12 1 53,1 10 26 0,00100132 157951639 4930404N11Rik uncharacterized protein C19orf71 homolog 1 35 5 7 1 24,3 4 7 1 35 5 14 1 35 5 28 0,00112962 157951670 CAPZA3 F-actin-capping protein subunit alpha-3 1 9,7 3 4 1 17,7 4 9 1 35,8 9 16 1 41,1 10 30 0,000755 157951743 H2-Ke6 estradiol 17-beta-dehydrogenase 8 1 47,5 8 12 1 49 8 13 1 62,9 10 16 1 62,9 10 42 0,00147613

157951745 Catsperg2 cation channel sperm-associated protein subunit gamma 2 precursor 1 5,9 6 9 1 4,5 4 4 1 3,1 3 3 1 9,3 9 16 0,000154 158187507 Fam71a uncharacterized protein LOC619288 1 9,6 5 15 1 11,6 6 13 1 22,6 10 36 1 24,3 11 64 0,000954 158341656 ODF1 outer dense fiber protein 1 1 15,7 4 9 1 40,7 8 26 1 43,5 9 25 1 43,5 9 60 0,00164635 158853992 Gm5506 alpha-enolase 1 50,7 16 126 1 45,9 13 79 1 59,2 18 124 1 59,2 18 280 0,00409918 158937279 mmel1 membrane metallo-endopeptidase-like 1 1 9,3 6 7 1 15,8 10 10 1 8,7 5 5 1 19,1 12 22 0,000255 160333212 Canx calnexin precursor 1 15,2 7 8 1 13,7 7 10 1 11,3 6 7 1 18,3 10 25 0,000476 160333353 trim69 E3 ubiquitin-protein ligase TRIM69 1 9 4 4 - 0 0 0 1 20 8 14 1 20 8 18 0,000266 160333553 rpl12 60S ribosomal protein L12 1 54,5 6 6 - 0 0 0 1 54,5 6 8 1 54,5 6 14 0,000497 160358791 AKAP3 A-kinase anchor protein 3 1 22,2 16 48 1 39,9 29 163 1 35,4 26 154 1 44,2 32 370 0,0028002 succinyl-CoA:3-ketoacid coenzyme A transferase 2B, mitochondrial 160358827 LOC100046138 precursor 1 60,2 21 151 - 0 0 0 0 0 0 0 1 74,4 29 888 0,00863379 160420308 Hist1h2bp histone H2B type 1-P 1 34,9 5 6 1 34,1 5 7 1 28,6 4 4 1 40,5 6 14 0,000608 160707894 Akr1b3 aldose reductase 1 5,4 3 3 1 21,8 6 7 1 32 8 9 1 38,3 10 21 0,000533 161016820 DNPEP aspartyl aminopeptidase isoform b 1 25,8 9 31 1 17,5 6 17 1 30,2 11 51 1 30,2 11 99 0,0021098 161016824 LOC100045796 cysteine desulfurase, mitochondrial 1 14,6 6 6 1 11,1 4 7 1 31,2 13 18 1 31,2 13 31 0,000471 161086957 4932412H11Rik leucine-rich repeat and death domain-containing protein 1 1 8,4 5 5 1 12,9 9 14 1 9 6 7 1 17,9 12 26 0,000282 161484642 LOC100044663 apolipoprotein O isoform 1 1 22 3 12 0,9997 15,9 2 3 1 41,5 5 15 1 41,5 5 30 0,00148861 161760667 lmna prelamin-A/C isoform C 1 15,2 8 9 - 1,7 1 2 0,9683 1,9 1 1 1 18,5 10 12 0,000123 162138915 cpt2 carnitine O-palmitoyltransferase 2, mitochondrial precursor 1 16,3 9 10 1 26,9 13 16 1 24,9 12 14 1 30,7 16 40 0,000511 162139827 ME1 NADP-dependent malic enzyme isoform 1 1 23,4 10 15 0,9993 2,9 2 2 1 34,1 14 16 1 40,4 17 34 0,000499 162287165 Cpt1b carnitine O-palmitoyltransferase 1, muscle isoform 1 9,8 7 7 1 24,2 16 23 1 19,3 13 15 1 31,2 21 47 0,000577 162287182 SPAG17 sperm-associated antigen 17 1 2 4 4 1 3,5 7 12 1 8,2 15 24 1 10,3 19 40 0,000174 162461907 HSPA9 stress-70 protein, mitochondrial 1 36,2 18 35 1 46,2 23 71 1 42,1 23 84 1 49 27 194 0,0018677 163310765 alb serum albumin precursor 1 15,8 9 23 1 23,4 13 13 1 16,9 9 21 1 29,4 16 57 0,000604 163644329 APOE apolipoprotein E precursor 1 12,9 4 6 1 12,9 4 5 1 17,7 5 5 1 20,6 6 16 0,000368 163838641 Atp5c1 ATP synthase subunit gamma, mitochondrial isoform a 1 44,2 9 24 1 28,1 7 31 1 44,2 9 48 1 44,2 9 102 0,00279509 163965436 Odf2 outer dense fiber protein 2 isoform b 1 38,9 22 73 - 0 0 0 0 0 0 0 0 0 28 0 - 164519120 CCDC42 coiled-coil domain-containing protein 42A 1 20,9 5 7 1 18,4 5 7 1 19,9 5 6 1 30,7 8 20 0,000434 164565394 DNAJC11 dnaJ homolog subfamily C member 11 1 7,9 4 4 1 9,5 5 5 1 19,9 9 15 1 19,9 9 24 0,000309 164607162 Dnalc1 dynein light chain 1, axonemal 1 29,5 5 8 1 29,5 5 8 1 29,5 5 15 1 29,5 5 31 0,00099 165932339 cutC copper homeostasis protein cutC homolog isoform 2 1 27,1 4 4 1 27,5 5 9 1 32,8 5 8 1 36,3 6 21 0,00057 165932389 NUP155 nuclear pore complex protein Nup155 1 5,2 5 5 1 3,9 4 7 1 11,9 12 14 1 13,8 14 26 0,000233 166851828 Atp5d ATP synthase subunit delta, mitochondrial precursor 1 13,7 2 8 0,9995 8,3 1 2 1 13,7 2 4 1 13,7 2 14 0,000987 167000069 TXNDC3 thioredoxin domain-containing protein 3 isoform 1 1 10,9 7 13 1 18,3 9 15 1 18,6 11 29 1 28,2 14 60 0,000944 16716343 Gm16399 cytochrome c oxidase subunit 6C 1 47,4 6 20 1 46,1 5 19 1 47,4 6 17 1 47,4 6 56 0,00504058 167234369 NHEDC1 sodium/hydrogen exchanger 9B1 1 14,5 7 15 1 12,6 6 7 1 16,5 8 14 1 17,9 9 36 0,000802 167716840 COX2 cytochrome c oxidase subunit II [Mus musculus musculus] 1 16,3 3 15 1 43,6 6 20 0 0 0 0 0 0 6 0 - 168229152 Gm13570 brain protein 44-like protein 2 1 43,7 4 13 1 26,1 3 5 1 63 5 15 1 63 5 33 0,0019948

170014720 BDH1 D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor 1 26,5 7 11 1 28,3 7 13 1 28,6 7 17 1 33,2 9 42 0,000786

170763467 Timm44 mitochondrial import inner membrane translocase subunit TIM44 1 23,5 9 14 1 14,2 5 8 1 39,2 16 26 1 39,2 17 48 0,000673 17647499 Hbb-b2 hemoglobin subunit beta-2 1 72,8 10 77 1 72,8 10 34 1 83,7 12 30 1 83,7 11 142 0,00485672 18079339 ACO2 aconitate hydratase, mitochondrial precursor 1 40,8 24 48 1 49,1 28 182 1 46,4 28 188 1 49,1 28 417 0,00437971 Réplica 1 Réplica 2 Réplica 3 Datos combinados

Probabilidad Números de Porcentaje Números de Porcentaje Números de Probabilidad Porcentaje Referencia de Porcentaje de péptidos Número de Probabilidad de péptidos Número de Probabilidad de péptidos Número de de de Números de Número de GI Proteína Nombre de la proteína coincidencia cobertura únicos espectros de coincidencia cobertura únicos espectros de coincidencia cobertura únicos espectros coincidencia cobertura péptidos únicos espectros Valor APEX pyruvate dehydrogenase E1 component subunit beta, mitochondrial 18152793 Gm6123 precursor 1 40,4 10 97 1 49,9 12 149 1 57,7 13 194 1 57,7 13 445 0,00867178 isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial 18250284 idh3a precursor 1 29 9 16 1 42,1 14 37 1 42,1 15 31 1 42,3 16 81 0,00161144 succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial 18266680 oxct1 precursor 1 7,9 3 3 - 0 0 0 1 18,1 7 14 1 21,9 8 17 0,000249 183396771 Gm12141 60 kDa heat shock protein, mitochondrial 1 50,3 22 73 1 37,7 16 70 1 54,8 27 162 1 54,8 27 308 0,00283314 18390323 Rab14 ras-related protein Rab-14 1 64,7 12 36 1 76,7 14 33 1 79,5 16 62 1 82,8 16 132 0,00364889 18700024 IDH3B isocitrate dehydrogenase 3, beta subunit 1 31,2 9 14 1 41,9 12 28 1 47,7 14 46 1 51,6 15 90 0,0018482 188035922 ACRBP acrosin-binding protein isoform 1 precursor 1 33,5 13 59 1 30,6 11 31 1 30,6 13 58 1 41,3 15 151 0,00287278 188035926 NDUFAF2 mimitin, mitochondrial 1 25,6 4 7 - 0 0 0 1 18,5 3 4 1 32,1 5 11 0,000357 glycerophosphodiester phosphodiesterase domain-containing 188219547 Gdpd4 protein 4 1 3,8 2 5 1 7,9 4 9 0,9867 2,1 1 3 1 10 5 17 0,0004 188219614 Uchl1 ubiquitin carboxyl-terminal hydrolase isozyme L1 1 21,1 3 3 1 24,7 4 5 0,9995 13 2 2 1 24,7 4 10 0,000375 188528624 prmt5 protein arginine N-methyltransferase 5 1 8,2 4 4 1 9,6 5 5 1 12,2 5 5 1 17,3 8 14 0,000207 18859597 LOC675851 NADH dehydrogenase [ubiquinone] 1 subunit C2 1 32 3 7 1 32 3 7 1 33 4 11 1 33 4 22 0,0014516 189409138 CAND1 cullin-associated NEDD8-dissociated protein 1 1 9,3 10 10 1 15 15 18 1 12,1 12 14 1 21,7 22 42 0,000297 191252810 TPPP2 tubulin polymerization-promoting protein family member 2 1 22,9 3 12 - 8,2 1 1 1 27,6 4 10 1 27,6 4 23 0,00065 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial 19526814 Ndufv1 precursor 1 22,2 8 9 1 10,8 5 10 1 50,4 16 39 1 52,6 17 57 0,000946 19526912 st13 hsc70-interacting protein 1 7,8 3 3 - 3,5 1 1 1 18,6 6 9 1 22,1 7 13 0,000316 19527102 STX12 syntaxin-12 1 38,7 8 13 1 21,5 4 5 1 49,3 9 14 1 49,3 9 31 0,00115096 19527224 CSNK1G2 casein kinase I isoform gamma-2 isoform 1 1 14,2 5 9 - 0 0 0 1 14,2 5 6 1 14,2 5 18 0,000392 19527228 CISD1 CDGSH iron-sulfur domain-containing protein 1 1 42,6 4 11 1 51,9 5 8 1 44,4 5 14 1 52,8 6 33 0,0019233 methylmalonate-semialdehyde dehydrogenase [acylating], 19527258 Aldh6a1 mitochondrial precursor 1 7,5 3 3 1 10,7 4 7 1 23,4 11 21 1 25,2 12 31 0,000447 19705424 psmd3 26S proteasome non-ATPase regulatory subunit 3 1 17,7 8 8 1 12,1 6 7 1 11,1 5 6 1 23,8 11 20 0,000263 isochorismatase domain-containing protein 2A, mitochondrial 197333728 Isoc2a precursor 1 35,9 4 4 1 43,7 5 7 1 42,7 5 13 1 51,5 6 24 0,00132103 197927139 LOC630540 WD repeat domain 63 1 4,1 3 3 1 12,9 11 29 1 16,3 13 16 1 20,3 16 48 0,000542 198278567 cpvl probable serine carboxypeptidase CPVL precursor 1 9 4 4 - 0 0 0 0,9994 4,2 2 2 1 10,7 5 7 0,00015 19882201 PSMD2 26S proteasome non-ATPase regulatory subunit 2 1 17,4 11 15 1 28,3 19 26 1 28,1 18 24 1 39,4 26 67 0,000668

205361112 DNAJA3 dnaJ homolog subfamily A member 3, mitochondrial isoform 1 1 9 4 5 - 0 0 0 1 8,3 3 3 1 12,3 5 8 0,00012 205361116 Mdh1b putative malate dehydrogenase 1B 1 7,8 4 4 - 0 0 0 1 8 4 4 1 11 5 8 0,000156 209862989 Uba1 ubiquitin-like modifier-activating enzyme 1 isoform 2 1 7,3 5 8 1 7,8 6 7 1 12,1 9 13 1 14,7 11 29 0,000269 21311859 Fam166a protein FAM166A 1 26,6 8 18 1 53,6 14 49 1 59,6 16 75 1 62,7 17 144 0,00390795

21312012 Ndufa8 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 1 29,1 4 8 - 0 0 0 1 59,3 7 21 1 59,3 7 28 0,00136371 21312181 LYZL4 lysozyme-like protein 4 precursor 1 33,8 3 4 1 24,1 3 4 1 40 4 5 1 40 4 13 0,000735 21312524 CYB5R1 NADH-cytochrome b5 reductase 1 1 13,4 3 3 - 3,6 1 1 0 0 0 0 1 13,4 3 4 0,000123 21312594 Gm3982 mitochondrial pyruvate carrier 2 1 51,2 5 21 1 27,6 3 6 1 38,6 5 30 1 52 6 58 0,00447549 21312834 TSSK4 testis-specific serine/threonine-protein kinase 4 isoform 1 1 34,8 10 12 1 31,4 9 11 1 30,5 9 12 1 38,1 11 35 0,000707 21312844 lrrc48 leucine-rich repeat-containing protein 48 1 22,6 9 9 - 3,3 1 1 1 18,2 7 7 1 26 11 17 0,000293 21312846 SPACA1 sperm acrosome membrane-associated protein 1 precursor 1 30,2 6 99 1 43,6 8 106 1 38,4 7 121 1 43,6 8 330 0,01534384 21312922 CCDC39 coiled-coil domain-containing protein 39 1 3,5 3 3 1 17,7 15 21 1 15,2 12 20 1 19,5 17 45 0,000316 NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, 21312950 NDUFS7 mitochondrial precursor 1 21,4 5 14 1 21,4 5 9 1 21,9 6 29 1 21,9 6 52 0,00201182 21312974 RSPH9 radial spoke head protein 9 homolog 1 58,7 13 23 1 48,9 11 19 1 55,1 12 39 1 69,9 16 80 0,00200432 21312994 Slc25a11 mitochondrial 2-oxoglutarate/malate carrier protein 1 28,3 8 8 1 13,7 4 5 1 28 9 9 1 40,8 11 22 0,000474 21313012 RAB4B ras-related protein Rab-4B 1 33,3 6 12 1 20,7 4 4 1 40,4 7 16 1 40,4 7 32 0,000832 21313054 IQCD IQ domain-containing protein D 1 13,8 6 8 0,9993 3,7 2 2 0,9991 4,1 2 2 1 13,8 6 12 0,000163 dihydrolipoyllysine-residue succinyltransferase component of 2- 21313536 DLST oxoglutarate dehydrogenase complex, mitochondrial 1 20,9 7 36 - 2,6 1 2 1 23,6 8 24 1 27,1 9 66 0,0012896 21313544 BCS1L mitochondrial chaperone BCS1 1 10,5 3 3 - 0 0 0 1 19,6 7 8 1 27,8 9 11 0,000213 coiled-coil-helix-coiled-coil-helix domain-containing protein 3, 21313618 CHCHD3 mitochondrial precursor 1 32,2 9 12 1 35,7 9 13 1 47,1 11 21 1 50,7 14 46 0,00123199 21313679 LOC674469 ATP synthase subunit d, mitochondrial 1 73,9 10 15 1 64,6 10 16 1 61,5 10 30 1 74,5 11 61 0,00297917 21313683 DPEP3 dipeptidase 3 precursor 1 31,2 11 32 1 29,8 10 26 1 31,2 11 45 1 32,7 12 105 0,00202074 21314832 UGP2 UTP--glucose-1-phosphate uridylyltransferase 1 13,2 6 6 - 0 0 0 0,9999 6,5 3 3 1 15,2 7 8 0,000114 21361209 Hist1h4c histone H4 1 50,5 5 9 1 42,7 4 5 1 43,7 5 6 1 51,5 6 20 0,00106379 21362335 CPA5 carboxypeptidase A5 precursor 1 7,1 3 3 - 1,8 1 1 1 9,2 4 4 1 11 5 8 0,000195 214010170 LOC100046120 clusterin precursor 1 9,8 4 14 1 14,5 5 11 1 18,8 7 16 1 23,2 8 41 0,000833 21450129 ACAT1 acetyl-CoA acetyltransferase, mitochondrial precursor 1 13,2 4 4 1 16 5 11 1 22,6 7 17 1 22,6 7 32 0,000682 21450187 CECR5 cat eye syndrome critical region protein 5 homolog precursor 1 15,3 5 5 - 0 0 0 1 29,1 9 10 1 25,5 8 14 0,000327 21539585 UQCRQ cytochrome b-c1 complex subunit 8 1 28 3 4 1 28 3 6 1 37,8 4 6 1 37,8 4 16 0,00122614 21539599 Uqcrh cytochrome b-c1 complex subunit 6, mitochondrial 1 37,1 2 4 1 44,9 3 4 0,9998 20,2 1 2 1 44,9 3 11 0,000783 21553309 APOA1BP NAD(P)H-hydrate epimerase precursor 1 14,9 3 5 1 28 7 10 1 33,7 7 11 1 36,9 9 26 0,000832 21703854 DHRS7B dehydrogenase/reductase SDR family member 7B isoform 2 1 19,1 4 8 1 22,6 5 7 1 15,9 4 5 1 27,1 6 20 0,000471 Réplica 1 Réplica 2 Réplica 3 Datos combinados

21703884 L2HGDH L-2-hydroxyglutarate dehydrogenase, mitochondrial precursor 1 10,6 4 5 1 12,1 4 10 1 29,5 12 31 1 29,5 12 46 0,000661 21703972 ME2 NAD-dependent malic enzyme, mitochondrial precursor 1 21,1 9 9 - 1,5 1 1 1 20 9 13 1 28 12 23 0,00034 bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP 21703976 dak lyase (cyclizing) 1 46 16 45 1 13,7 5 5 1 37,5 14 33 1 47,4 17 85 0,00135179 21704100 Gm13910 trifunctional enzyme subunit beta, mitochondrial precursor 1 24,4 10 10 1 11,4 5 10 1 43,6 14 21 1 47,6 16 41 0,000762

21704140 hibadh 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor 1 35,2 8 17 1 40,6 8 18 1 55,5 11 30 1 55,5 11 65 0,0017461 21729773 1700001C02Rik UPF0573 protein C2orf70 homolog 1 26 5 13 1 22,5 4 6 1 31 7 17 1 31 7 36 0,00130794 22094075 LOC631229 ADP/ATP translocase 2 1 17,8 5 5 0,9997 17,4 5 6 1 34,6 9 12 1 34,6 9 22 0,00043 mitochondrial import inner membrane translocase subunit TIM50 22094989 timm50 precursor 1 13,3 4 5 1 19 5 6 1 24,6 7 9 1 27,8 8 20 0,0005 22122625 HIBCH 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial precursor 1 11,2 4 4 1 23,9 8 11 1 21,3 7 7 1 26,8 9 22 0,000393 22128627 sorD sorbitol dehydrogenase 1 48,5 13 60 1 47,6 13 73 1 53,5 14 60 1 53,5 14 194 0,00440688 22165382 csnk1a1 casein kinase I isoform alpha 1 24,3 6 8 1 19,4 5 9 1 36,3 9 18 1 36,3 9 35 0,000783 22165384 TUBB2C tubulin beta-4B chain 1 67 21 105 1 63,1 21 72 1 76 25 172 1 76 25 361 0,00445572 22267442 Uqcrc2 cytochrome b-c1 complex subunit 2, mitochondrial precursor 1 44,6 17 111 1 40,6 16 69 1 49 18 112 1 49 18 293 0,00586503 223941805 Gm9047 uncharacterized protein LOC668210 1 20,9 4 9 1 16,4 3 7 1 20,9 4 9 1 20,9 4 26 0,00092 223972700 Fam71e2 uncharacterized protein LOC243822 1 5,6 4 8 1 4,4 3 5 1 5,3 4 9 1 8,4 6 22 0,000296 224809376 NCSTN nicastrin precursor 1 9,5 6 10 1 6,4 4 7 1 14,7 8 12 1 16 9 29 0,000454

224922803 Gpd2 glycerol-3-phosphate dehydrogenase, mitochondrial precursor 1 12,9 8 9 1 52,5 29 66 1 59,6 33 87 1 64,6 36 164 0,0011903 225007547 GSTO2 glutathione S-transferase omega-2 1 28,2 7 11 1 31 8 10 1 31 7 15 1 31 8 35 0,00121139 22507355 SLC8A2 sodium/calcium exchanger 2 precursor 1 9,9 6 7 1 13,7 8 10 1 6,7 4 4 1 15,6 9 21 0,000288

22550094 PRKAR2A cAMP-dependent protein kinase type II-alpha regulatory subunit 1 45,5 14 34 1 40,8 12 46 1 44,5 15 103 1 48 16 184 0,00355626

22550102 PLCZ1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 1 8,5 4 4 0,9997 4,5 2 3 1 7,9 4 4 1 12,2 6 11 0,000185 225543319 LOC675857 transitional endoplasmic reticulum ATPase 1 54,5 28 38 1 55,1 34 61 1 53,3 31 65 1 63 39 163 0,00148898 225543354 Zmynd12 zinc finger, MYND domain containing 12 1 19,3 5 5 1 15,7 4 4 1 22,6 6 10 1 25,9 7 19 0,000382 225543576 gtl3 UPF0468 protein C16orf80 homolog 1 27,5 6 10 1 20,2 4 6 1 32,1 8 18 1 32,1 8 34 0,00123552 225690614 ACOT7 cytosolic acyl coenzyme A thioester hydrolase isoform 2 1 14,6 4 4 1 26,6 6 8 0,9995 8,2 2 3 1 32,5 8 16 0,000396 225735582 HK1 hexokinase-1 isoform HK1-sb 1 44,6 38 216 1 42,1 35 210 1 50,3 41 269 1 50,3 41 653 0,00392966 226246531 PGK2 phosphoglycerate kinase 2 1 45,8 14 93 1 43,9 13 46 1 59,5 17 84 1 63,1 19 231 0,0038228 226442909 NIPSNAP3A nipsnap homolog 3A 1 34,7 7 17 1 34,7 7 17 1 31 6 22 1 34,7 7 55 0,0024728 226442916 spata18 mitochondria-eating protein 1 4,8 2 8 1 14,7 6 16 1 20,9 10 39 1 20,9 10 63 0,00129762 226442984 PTCHD3 patched domain-containing protein 3 1 14,9 11 20 1 14,8 11 24 1 15,8 12 19 1 17,7 13 62 0,0010541 226443038 Glipr1l3 GLI pathogenesis-related 1 like 1 precursor 1 16,1 4 7 1 17,4 4 8 1 21,6 5 6 1 25 6 21 0,000773 226530884 pcmt1 protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 20 4 4 1 24,2 4 5 0,9997 10,5 2 3 1 36,8 6 12 0,000331 226530999 DNAJB3 dnaJ homolog subfamily B member 3 1 12,8 3 3 1 19 4 4 1 16,9 4 4 1 21,9 5 11 0,000343 226693361 CCT6B T-complex protein 1 subunit zeta-2 1 13,6 5 3 - 0 0 0 1 22 9 6 1 23,5 10 10 0,00014 226823367 Fh1 fumarate hydratase, mitochondrial precursor 1 12,2 5 7 1 8,7 3 6 1 30,8 10 14 1 32,3 11 27 0,000477 226958349 TPI1 triosephosphate isomerase 1 21,1 5 8 1 45,2 11 30 1 51,8 11 29 1 54,8 13 71 0,00156443 226958351 ATP1A4 sodium/potassium-transporting ATPase subunit alpha-4 1 39,8 34 82 1 37 32 114 1 37 34 101 1 42,4 37 280 0,00263907 226958374 Sypl synaptophysin-like protein 1 isoform 1 1 18,9 3 7 0,9992 14 2 10 1 18,9 3 7 1 18,9 3 26 0,00146316 cytochrome c oxidase subunit 7A-related protein, mitochondrial 226958512 Cox7a2l isoform 1 1 48,6 3 4 1 48,6 3 3 0,9993 19,8 2 3 1 48,6 3 10 0,000731 227116358 MECR trans-2-enoyl-CoA reductase, mitochondrial precursor 1 26 7 14 1 38,9 10 23 1 38,6 10 18 1 48,5 12 58 0,00134457 227330593 Dnaic1 dynein intermediate chain 1, axonemal 1 14,6 8 12 1 27,8 16 31 1 37,5 20 37 1 42,4 23 80 0,000975 227330625 MAN2B2 epididymis-specific alpha-mannosidase precursor 1 18,8 13 40 1 21,5 17 32 1 25,7 18 35 1 27,1 20 108 0,00145872 227496048 Gm5347 a disintegrin and metallopeptidase domain 34 1 8,5 5 6 1 4,9 3 5 1 5,6 3 3 1 8,5 5 14 0,000229 227908775 RSPH6A radial spoke head protein 6 homolog A isoform 1 1 17,5 8 17 1 24,9 11 22 1 20,6 9 36 1 27,4 12 77 0,00188381 228008337 psmc3 26S protease regulatory subunit 6A 1 13,6 4 5 0,9992 6,6 2 2 1 8,4 3 4 1 20,8 6 11 0,000164 228480241 MTX2 metaxin-2 1 19 4 10 1 20,2 4 7 1 26,6 5 10 1 30,4 6 28 0,00118847 228480264 Spaca4 sperm acrosome membrane-associated protein 4 precursor 1 40,9 4 9 1 33,1 3 6 1 33,1 3 6 1 40,9 4 21 0,0017906 229093139 HTRA1 serine protease HTRA1 precursor 1 7,1 3 7 - 2,9 1 2 1 12,9 4 8 1 12,9 4 17 0,000296 229577240 Zp3r zona pellucida sperm-binding protein 3 receptor precursor 1 14,2 6 24 1 20,2 8 53 1 20,4 9 56 1 24,9 10 132 0,00264288 229608935 NME7 nucleoside diphosphate kinase 7 isoform 1 1 10,9 4 7 - 0 0 0 1 36,7 10 14 1 39 11 21 0,000414 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial 229892316 NDUFS1 precursor 1 19,5 10 17 1 47,7 26 81 1 51,4 28 116 1 56,5 30 213 0,00209048 NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, 23346461 ndufs2 mitochondrial precursor 1 14,3 6 8 0,9996 4,3 2 4 1 43,2 16 40 1 43,2 16 52 0,0010109 23346525 CD109 CD109 antigen precursor 1 9,7 10 16 1 15,4 18 40 1 13,9 15 32 1 17,1 20 89 0,000693 23346533 PHOSPHO1 phosphoethanolamine/phosphocholine phosphatase 1 20,2 5 5 1 23,6 7 13 1 35,6 10 21 1 36 11 39 0,00102924 23346595 2410015M20Rik protein QIL1 precursor 1 41,2 3 4 - 18,5 1 1 1 46,2 2 3 1 68,9 4 8 0,000605 amyotrophic lateral sclerosis 2 (juvenile) chromosome region, 237649035 Als2cr11 candidate 11 1 11 5 11 0,9995 4,3 2 3 1 10,2 3 6 1 16,3 6 20 0,00031 238231384 PSMB6 proteasome subunit beta type-6 precursor 1 16,8 4 5 0,9994 8,8 2 2 1 16,8 4 6 1 16,8 4 14 0,000502 238231425 KRT35 keratin, type I cuticular Ha5 1 8,8 4 3 - 0 0 0 0 0 0 0 1 8,8 4 3 0,0000463 Réplica 1 Réplica 2 Réplica 3 Datos combinados

239052655 EFCAB6 EF-hand calcium-binding domain-containing protein 6 isoform 1 1 15,1 11 22 1 24,9 17 40 1 27,4 18 64 1 28,6 19 129 0,000586 23943818 ccdc65 coiled-coil domain-containing protein 65 1 14,6 6 6 - 0 0 0 1 16 7 7 1 22,3 9 13 0,000189 23943838 slc25a1 solute carrier family 25, member 1 1 10,3 3 3 1 12,2 4 5 1 15,8 5 5 1 21,5 7 13 0,000318 23956396 Erlin2 erlin-2 1 26,8 7 13 0,9994 7,1 2 3 1 30,6 9 10 1 40 11 26 0,000548 240120054 Akr7a5 aflatoxin B1 aldehyde reductase member 2 1 12,5 4 5 1 20,7 5 6 1 19,1 5 7 1 27 8 18 0,000531 241982800 1700080O16Rik uncharacterized protein LOC74279 1 9,2 3 5 1 10,6 3 4 1 15,8 5 8 1 20,1 6 17 0,000451 242247109 CYLC2 cylicin, basic protein of sperm head cytoskeleton 2 1 11,1 3 24 1 11,4 4 5 1 11,4 4 14 1 14 5 43 0,00077 244790045 SQRDL sulfide:quinone oxidoreductase, mitochondrial 1 7,3 3 3 0,9994 4,9 2 2 1 29,6 11 12 1 31,1 12 17 0,00024 244791124 DPP3 dipeptidyl peptidase 3 1 5,1 3 3 1 16 8 10 1 7 3 5 1 21,1 11 18 0,000195 247270151 ftmt ferritin, mitochondrial precursor 1 8,4 2 3 1 13,9 3 5 1 36,3 6 14 1 36,3 6 21 0,000718 25072207 Dynlrb2 dynein light chain roadblock-type 2 1 43,8 3 4 1 43,8 3 6 1 43,8 3 4 1 43,8 3 14 0,000866 251823722 1700023D19Rik izumo sperm-egg fusion protein 2 precursor 1 23,3 4 13 - 4,1 1 1 1 17,6 3 15 1 28,5 5 29 0,00128886 253795525 CCDC147 coiled-coil domain containing 147 1 16,8 15 22 1 17 13 28 1 25,9 23 40 1 26 24 90 0,000641 253795534 FSCB fibrous sheath CABYR-binding protein 1 12,1 8 16 1 25 17 46 1 14,4 11 19 1 25,9 19 82 0,00145429 254281222 HTRA2 serine protease HTRA2, mitochondrial 1 42,6 12 17 1 29,7 10 16 1 29,7 10 18 1 47,8 15 52 0,00104876 254540027 Mdh1 malate dehydrogenase, cytoplasmic 1 16,5 5 5 1 22,8 6 6 1 23,1 6 7 1 28,7 8 18 0,000403 254540068 KRT18 keratin, type I cytoskeletal 18 1 23,9 10 8 - 0 0 0 0 0 0 0 1 23,9 10 8 0,000121 254540082 PSMB4 proteasome subunit beta type-4 precursor 1 24,6 4 6 1 27,3 4 6 1 42 6 12 1 42 6 24 0,000827 254540166 HSPA5 78 kDa glucose-regulated protein precursor 1 29,9 14 13 1 38,9 23 36 1 25,8 13 13 1 38,9 23 63 0,000671 254553303 DYDC1 DPY30 domain-containing protein 1 1 35,4 6 11 1 21,7 4 6 1 35,4 6 11 1 35,4 6 29 0,001104 254553376 CCDC105 coiled-coil domain-containing protein 105 1 9,8 4 5 1 33,3 16 42 1 37,5 18 61 1 39,7 19 109 0,00145789 254553422 EFHC1 EF-hand domain-containing protein 1 1 20,1 11 12 1 35 16 32 1 37,7 18 34 1 52 25 79 0,000843 254553434 IL4I1 L-amino-acid oxidase precursor 1 26,8 13 29 1 8,9 5 5 1 31,7 16 40 1 31,7 16 74 0,00120127 254553458 gpi1 glucose-6-phosphate isomerase 1 35,5 15 32 1 24,9 10 22 1 41,6 18 47 1 40,5 18 105 0,00187984 serine/threonine-protein phosphatase PGAM5, mitochondrial 254587960 pgam5 isoform 2 1 24 7 8 1 11,5 3 4 1 37,6 8 8 1 41,5 10 20 0,000429 254588114 Ttc39d tetratricopeptide repeat domain 39D 1 5,6 3 3 1 18 8 14 1 17,1 7 14 1 22,8 10 29 0,000445 254675102 RTDR1 rhabdoid tumor deletion region gene 1 isoform 1 1 14,4 4 4 1 24 7 8 1 19,1 6 9 1 24 7 21 0,000429 254675124 Ccdc19 nasopharyngeal epithelium specific protein 1 1 8,3 3 10 1 16 9 23 1 17,2 9 21 1 22,3 12 54 0,000629 254675150 hspb9 heat shock protein beta-9 1 22,6 4 14 - 5 1 1 1 47,7 8 23 1 47,7 8 38 0,00121261 254675162 NUP210L nuclear pore membrane glycoprotein 210-like precursor 1 3,4 6 7 1 10,1 15 19 1 9,8 13 13 1 14,2 20 38 0,000196 254675164 Tmem190 transmembrane protein 190 precursor 1 15,1 2 9 - 10,2 1 3 1 15,1 2 9 1 15,1 2 21 0,00190906 254675166 ak7 adenylate kinase 7 1 22,5 11 18 1 19,9 11 17 1 31,5 17 31 1 36,7 19 65 0,000768 254675236 KIF9 kinesin-like protein KIF9 isoform 2 1 12,5 8 12 - 0 0 0 1 11,6 6 9 0 0 11 0 - 254675296 PROM1 prominin-1 isoform s1 precursor 1 6,1 4 4 1 8,7 5 7 1 10,7 6 9 1 16,7 9 20 0,000257 254675347 glb1l beta-galactosidase-1-like protein precursor 1 14,2 7 8 - 1,5 1 1 1 16,1 8 8 1 20,7 11 17 0,000234 254692831 PSMA8 proteasome subunit alpha type-7-like 1 38,4 7 11 1 38 8 16 1 39,6 7 15 1 48,8 10 42 0,00107388 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, 254692859 NDUFA9 mitochondrial precursor 1 23,3 7 15 1 19,4 7 16 1 31,6 11 32 1 31,6 11 64 0,00126258 254692892 Slc25a31 ADP/ATP translocase 4 1 38,1 12 30 1 43,1 14 58 1 43,1 14 77 1 43,1 14 167 0,00277434 254692960 msto1 protein misato homolog 1 1 8,6 4 4 - 0 0 0 0 0 0 0 1 8,6 4 4 0,000061 254826790 slc25a10 mitochondrial dicarboxylate carrier 1 34,1 8 16 1 37,6 10 16 1 51,2 12 25 1 59,6 14 58 0,00147396 254910995 Naglu alpha-N-acetylglucosaminidase precursor 1 25,3 15 25 1 24,1 14 23 1 25,2 15 19 1 29,2 17 68 0,000936 254911018 ATP6V1E2 V-type proton ATPase subunit E 2 1 14,6 3 3 - 0 0 0 1 14,2 3 3 1 18,1 4 6 0,00016 255069715 LAP3 cytosol aminopeptidase 1 41,6 17 29 1 22,2 9 23 1 49,5 20 83 1 47,4 20 135 0,00172969 255683347 CCDC151 coiled-coil domain-containing protein 151 isoform 2 1 7,3 4 5 1 21,2 11 21 1 20,2 10 15 1 28 14 41 0,000425 succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, 255958286 suclg1 mitochondrial precursor 1 29,8 8 50 1 35,3 10 76 1 35,5 11 122 1 38,2 12 251 0,00893407 256000803 1700006E09Rik uncharacterized protein C17orf105 homolog 1 23,2 3 6 - 6,1 1 1 1 29,3 4 9 1 29,3 4 16 0,000624 256220343 Dhrs4 dehydrogenase/reductase SDR family member 4 isoform 1 1 34,8 8 12 1 31,9 8 13 1 38,4 10 20 1 38,4 10 46 0,00109155 256665241 Zan zonadhesin precursor 1 16,9 69 150 1 26,3 95 224 1 23,6 86 174 1 29,2 108 555 0,00110264 256818776 Wdr52 WD repeat-containing protein 52 1 2,9 4 4 1 13,5 18 28 1 12,2 18 38 1 16,4 24 73 0,000395 257743039 LdhA L-lactate dehydrogenase A chain isoform 2 1 22,9 7 27 1 29,4 10 50 1 41,8 14 71 1 41,8 14 136 0,00222475 dihydrolipoyllysine-residue acetyltransferase component of pyruvate 257796245 dlaT dehydrogenase complex, mitochondrial 1 41 21 123 1 29,8 15 81 1 42,8 20 180 1 48,8 23 384 0,00465206

257900479 CATSPERB cation channel sperm-associated protein subunit beta precursor 1 5,8 6 10 1 2,7 3 3 1 5,6 6 6 1 7,4 8 19 0,000163

260593706 Heatr7b2 maestro heat-like repeat-containing protein family member 2B 1 10,1 14 21 1 18,5 26 62 1 23,7 28 42 1 26,8 35 125 0,000636

261245039 TMEM146 cation channel sperm-associated protein subunit delta precursor 1 9,4 5 7 1 10,9 5 6 1 8,3 4 4 1 13,9 7 16 0,000279 261399871 CCDC33 coiled-coil domain-containing protein 33 isoform 1 1 6,8 3 3 - 0 0 0 0 0 0 0 1 6,8 3 3 0,0000274 261824000 Psma3 proteasome subunit alpha type-3 1 27,8 6 12 1 25,1 5 8 1 16,1 4 7 1 34,9 8 27 0,000637

261862282 SLC2A3 solute carrier family 2, facilitated glucose transporter member 3 1 9,9 5 26 1 14 7 34 1 10,1 6 31 1 16 8 93 0,00335785 261862311 CCDC11 coiled-coil domain-containing protein 11 1 7,8 3 3 1 11,3 5 8 1 24,7 10 12 1 28,2 12 24 0,000297 262050574 EFHC2 EF-hand domain-containing family member C2 1 8,9 5 5 1 21,5 15 30 1 37,7 23 32 1 35,7 23 67 0,000687 Réplica 1 Réplica 2 Réplica 3 Datos combinados

27370380 4933405O20Rik probable isocitrate dehydrogenase [NAD] gamma 2, mitochondrial 1 14,6 5 6 1 27,5 8 13 1 30,8 9 22 1 30,8 9 42 0,000935 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, 27754144 Ndufb5 mitochondrial precursor 1 19,6 3 9 - 5,8 1 1 1 23,8 4 14 1 23,8 4 24 0,000855 27923590 Adam4 a disintegrin and metallopeptidase domain 4 precursor 1 8,7 6 9 1 9,7 7 4 1 10,4 7 14 1 11,5 8 15 0,000174 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, 281485615 NDUFS4 mitochondrial 1 37,1 6 8 - 5,7 1 1 1 37,1 6 10 1 37,1 6 19 0,000609 28173550 Sept7 septin-7 isoform 1 1 13,1 5 5 - 2,3 1 1 1 29,8 10 12 1 31,4 10 17 0,000269 28201978 PDHX pyruvate dehydrogenase protein X component, mitochondrial 1 32,5 11 27 1 30,9 10 44 1 35,5 12 61 1 35,5 12 131 0,00215737 283046757 HEATR7A HEAT repeat containing 7A isoform 1 1 4,1 6 8 1 3,7 5 6 1 7 10 12 1 9,2 13 28 0,00016 monofunctional C1-tetrahydrofolate synthase, mitochondrial 283135110 MTHFD1L precursor 1 5,6 4 4 1 20,9 18 32 1 15,8 13 15 1 24,9 21 52 0,000391 28316764 ANKRD5 ankyrin repeat and EF-hand domain-containing protein 1 1 6,2 4 4 1 9,8 6 12 1 15,5 10 12 1 19,9 13 30 0,000261 285401645 1700001C19Rik uncharacterized protein LOC75462 isoform 2 1 40 4 4 1 29,6 3 3 1 32,2 3 3 1 40 4 10 0,000552 29243946 Thnsl1 threonine synthase-like 1 isoform a 1 5,5 3 3 1 25 14 18 1 31,6 17 24 1 36,9 20 47 0,000498 29244338 4933400C05Rik spermatogenesis-associated protein 32 1 13,5 3 3 1 23,1 6 17 1 25,1 7 22 1 25,1 7 41 0,0011809 29244434 BPI bactericidal permeability-increasing protein precursor 1 8,4 3 3 - 3,1 1 1 1 8,4 3 5 1 8,4 3 9 0,000185 293597567 Aldoa fructose-bisphosphate aldolase A isoform 1 precursor 1 60 16 19 1 63,4 20 24 1 83,7 25 57 1 83,7 27 97 0,00108909 29366814 Fahd2a fumarylacetoacetate hydrolase domain-containing protein 2A 1 53,7 9 16 1 57,2 11 26 1 57,2 12 44 1 57,2 12 89 0,00253361 295317325 snap23 synaptosomal-associated protein 23 isoform a 1 25,2 4 6 1 34,3 5 6 0,9831 6,7 1 1 1 34,3 5 14 0,000394 295444814 Prss46 serine protease 46 1 17,2 5 15 1 17,2 5 21 1 17,2 5 16 1 17,2 5 54 0,0022522 29648322 DNAJB13 dnaJ homolog subfamily B member 13 1 33,2 8 14 1 28,5 7 18 1 33,5 9 28 1 33,5 9 60 0,00121811 297747362 zpbp1 zona pellucida-binding protein 1 isoform 2 1 40,5 12 53 1 61,8 17 111 1 53,3 14 91 1 61,8 17 257 0,00710688

29789148 NDUFB9 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 1 49,2 6 10 1 51,4 6 12 1 65,4 8 21 1 65,4 8 41 0,00187838 29789217 TSNAXIP1 translin-associated factor X-interacting protein 1 1 4,7 3 3 1 8,5 5 8 1 14,5 9 10 1 14,5 9 21 0,00021 298231198 Nup53 nucleoporin NUP53 isoform 2 1 14,9 3 3 1 23,4 5 9 1 25 5 8 1 29,2 6 21 0,000774 30172570 DNALI1 axonemal dynein light intermediate polypeptide 1 1 45 10 20 1 47,7 12 30 1 61,2 16 47 1 61,2 16 98 0,00232599 30410018 Iqcg IQ domain-containing protein G 1 7,6 3 3 0,9992 5 2 2 1 7,6 3 6 1 7,6 3 11 0,00022 30424705 Tmem177 transmembrane protein 177 1 9 3 5 1 14,8 5 6 1 20,3 6 8 1 23,2 7 19 0,000588 30425048 LDHAL6B L-lactate dehydrogenase A-like 6B 1 35,1 10 74 1 52,4 13 181 1 53,1 13 166 1 55,2 14 424 0,00651498 30425148 CCDC40 coiled-coil domain-containing protein 40 1 5,2 4 5 1 14,2 13 22 1 11,9 12 16 1 18 17 43 0,000326 30519943 Samm50 sorting and assembly machinery component 50 homolog 1 46,7 15 15 - 0 0 0 1 46,7 15 24 1 46,7 15 39 0,000671 30520019 sccpdh saccharopine dehydrogenase-like oxidoreductase 1 33,6 10 32 1 38,7 11 43 1 58,7 14 83 1 61,8 15 159 0,00315341

30794476 PRKAR1A cAMP-dependent protein kinase type I-alpha regulatory subunit 1 27,3 10 14 1 15,5 4 5 1 31,8 12 23 1 31,8 12 43 0,00092 PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur 309265938 Ndufs5 protein 5-like 1 41,5 4 4 1 23,6 2 3 1 41,5 4 6 1 41,5 4 13 0,000688

312222784 Vdac3 voltage-dependent anion-selective channel protein 3 isoform 1 1 21,2 4 4 - 0 0 0 0 0 0 0 0 0 10 0 - 312922382 aldoa1a aldolase 1 A retrogene 1 1 29,4 9 3 1 39,4 12 4 1 55,6 18 19 1 62,8 20 27 0,000355 31542427 LOC677611 casein kinase II subunit alpha 1 26,3 7 10 - 0 0 0 1 22,5 6 9 1 31,5 8 21 0,000521 31542438 LOC100047577 cytochrome b5 type B precursor 1 32,2 2 3 - 0 0 0 1 63,7 7 23 1 63,7 7 24 0,001506 31543255 MME neprilysin 1 15,3 10 13 1 20,8 13 17 1 14 9 13 1 24,3 15 43 0,000482 31543608 RTN4IP1 reticulon-4-interacting protein 1, mitochondrial precursor 1 11,1 3 3 1 9,6 3 3 1 19,4 5 5 1 25,8 7 12 0,000217

31543728 SLC2A5 solute carrier family 2, facilitated glucose transporter member 5 1 10,6 5 21 1 10,8 6 16 1 10,6 5 22 1 10,8 6 58 0,00237532 31543773 SSSCA1 Sjoegren syndrome/scleroderma autoantigen 1 homolog 1 27,1 3 3 0,9994 22,1 2 3 1 31,2 4 6 1 31,2 4 12 0,000613 31560090 PLBD2 putative phospholipase B-like 2 precursor 1 8,6 4 4 1 10,8 5 7 1 16,2 7 7 1 20,5 9 18 0,000339 Réplica 1 Réplica 2 Réplica 3 Datos combinados

31980772 LOC627816 serine/threonine-protein phosphatase PP1-gamma catalytic subunit 1 27,2 7 13 0,9993 23,5 6 3 1 21,7 6 12 1 30,3 8 13 0,000322 31980844 dhrs1 dehydrogenase/reductase SDR family member 1 1 29,4 8 8 1 22,7 6 13 1 39 10 13 1 32,9 9 33 0,000699 31980939 1700020C11Rik mitochondrial fission process protein 1 1 21,7 4 7 - 0 0 0 1 28,9 6 11 1 28,9 6 18 0,000864 31980942 IMPA1 inositol monophosphatase 1 1 22,4 6 9 1 42,6 10 19 1 40,4 10 17 1 45,1 11 45 0,00117128 31980998 acot9 acyl-coenzyme A thioesterase 9, mitochondrial 1 26,7 11 15 1 20,5 9 29 1 45,1 18 68 1 45,1 18 108 0,00170404

31981600 Ndufa2 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 1 41,4 4 5 1 33,3 3 3 1 41,4 4 5 1 41,4 4 13 0,000689 31981810 dci enoyl-CoA delta isomerase 1, mitochondrial precursor 1 16,3 4 6 1 18,3 5 6 1 28 8 12 1 28 8 25 0,000702 31981922 LOC100048601 probable threonine protease PRSS50 precursor 1 8,7 3 8 0,9992 4,1 2 3 1 7,3 3 8 1 12,3 5 19 0,000562 31982091 Gm12906 mitochondrial import receptor subunit TOM22 homolog 1 47,9 4 6 - 9,2 1 1 1 62 5 8 1 62 5 15 0,00103058 31982186 mdh2 malate dehydrogenase, mitochondrial precursor 1 54,1 14 38 1 58,3 15 65 1 58,3 15 82 1 58,3 15 189 0,00377932 31982856 dld dihydrolipoyl dehydrogenase, mitochondrial precursor 1 42,2 14 42 1 19,1 7 23 1 50,7 17 99 1 50,7 17 166 0,00267713 N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase isoform 2 326439050 - precursor 1 11,3 3 4 - 0 0 0 0 0 0 0 0 0 6 0 - 33147082 bat3 large proline-rich protein BAG6 isoform 1 1 7,9 7 9 1 10,6 9 15 1 13,2 9 10 1 17,4 13 35 0,000545 33354251 Lypd4 ly6/PLAUR domain-containing protein 4 precursor 1 41,1 7 18 1 35 6 17 1 41,1 7 22 1 41,1 7 58 0,00222846 33468873 ACE angiotensin-converting enzyme isoform 2 precursor 1 20,9 13 30 - 0 0 0 0 0 0 0 0 0 22 0 - 334688818 Wdr78 WD repeat-containing protein 78 1 4,5 3 4 1 10,5 8 11 1 13,4 9 12 1 18,8 13 27 0,000284

33468937 TIMM17B mitochondrial import inner membrane translocase subunit Tim17-B 1 21,5 3 4 - 0 0 0 1 21,5 3 4 1 21,5 3 8 0,000409 33469015 prcp lysosomal Pro-X carboxypeptidase precursor 1 15,1 6 7 - 0 0 0 1 13,4 6 9 1 15,3 7 17 0,000419 33563282 PSMA1 proteasome subunit alpha type-1 1 26,6 6 6 1 43,7 12 16 1 43,3 11 13 1 46,4 13 35 0,00110216 336088630 Nit1 nitrilase homolog 1 isoform 2 1 32,8 6 6 1 28,3 6 8 1 57,2 12 16 1 57,2 12 31 0,000873 33859530 cryz quinone oxidoreductase 1 23,9 7 7 1 28,4 7 9 1 30,5 9 9 1 41,1 12 26 0,000571 33859604 PSMC2 26S protease regulatory subunit 7 1 24,2 10 10 1 6,9 3 5 1 10,3 4 4 1 26,3 11 20 0,000237 33859638 THEG testicular haploid expressed gene protein isoform 1 1 14,8 5 13 1 16 6 15 1 27,7 11 41 1 27,7 11 69 0,00154415 33942057 SPINT4 kunitz-type protease inhibitor 4 precursor 1 25,2 3 5 - 0 0 0 0,9994 11,9 2 3 1 25,2 3 8 0,000371 34303985 CCDC63 coiled-coil domain-containing protein 63 1 24,4 12 17 1 15,8 11 31 1 24,2 16 47 1 28,5 18 97 0,00112243 34328230 Ak2 adenylate kinase 2, mitochondrial isoform b 1 38,8 8 13 1 42,2 9 26 1 42,2 9 43 1 42,2 9 82 0,00185879 succinate dehydrogenase [ubiquinone] iron-sulfur subunit, 34328286 LOC638710 mitochondrial precursor 1 35,5 9 18 1 39,4 9 29 1 42,9 10 42 1 42,9 10 89 0,00164564 34328288 Aldh3b1 aldehyde dehydrogenase family 3 member B1 1 14,5 6 6 - 0 0 0 1 9,6 4 4 1 14,5 6 10 0,000178 34536823 Cox6b2 cytochrome c oxidase subunit 6B2 isoform 2 1 47,7 4 6 1 47,7 4 9 1 47,7 4 7 1 47,7 4 22 0,00197485 356582477 Ogdh 2-oxoglutarate dehydrogenase, mitochondrial isoform 1 1 20,3 17 33 1 45,3 35 129 1 52,4 45 124 1 55,3 46 242 0,0021257 357588427 Serpina1a alpha-1-antitrypsin 1-1 isoform 2 1 11,2 4 5 - 0 0 0 0 0 0 0 1 11,2 4 5 0,000159 357933645 Ptgr2 prostaglandin reductase 2 isoform 1 1 10,5 3 3 1 11,1 3 4 0,9991 8 2 2 1 16,5 5 9 0,000245 358439483 Immt mitochondrial inner membrane protein isoform 2 1 43,2 25 56 1 55,5 36 126 1 54,3 39 138 1 60,2 42 44 0,000401 359385696 Ywhaz 14-3-3 protein zeta/delta isoform 1 1 40 8 24 1 40 10 29 1 43,7 10 26 1 44,1 11 80 0,00233961 359807367 Pkm pyruvate kinase, muscle isoform M1 1 54,8 25 84 1 52,7 24 155 1 67,2 31 349 1 66,1 30 581 0,00565392 37574048 Gm6293 cytochrome b-c1 complex subunit 9 1 37,5 2 5 0,9988 37,5 2 2 1 37,5 2 4 1 37,5 2 11 0,00148412 PREDICTED: orphan sodium- and chloride-dependent 377834273 Ntt5 neurotransmitter transporter NTT5 1 9,4 4 7 1 5,4 3 7 1 7,4 3 7 1 9,4 4 21 0,000579 38142460 LOC100045699 electron transfer flavoprotein subunit beta 1 11,4 3 3 1 23,5 5 6 1 36,5 8 8 1 40 9 17 0,000358 38348482 CCT8L1 chaperonin containing TCP1, subunit 8 (theta)-like 1 1 16,9 9 9 - 2,5 1 2 1 23,8 11 11 1 25,3 12 22 0,000354 384871588 Ace3 angiotensin I converting enzyme 3 precursor 1 28,8 17 36 1 23,6 16 32 1 24,3 15 30 1 30,3 19 98 0,00127884 38490690 iars2 isoleucine--tRNA ligase, mitochondrial precursor 1 2,8 3 3 1 24,7 21 46 1 31,1 26 49 1 33,6 28 100 0,000876 39930543 NUP54 nuclear pore complex protein Nup54 1 17,1 6 7 - 0 0 0 1 17,8 6 6 1 22,7 9 13 0,000223 40254361 Hspa4l heat shock 70 kDa protein 4L 1 21,5 14 20 1 31,5 19 39 1 31,4 18 27 1 47,9 28 87 0,000719 40254574 FABP9 fatty acid-binding protein 9 1 53 7 20 1 63,6 8 39 1 63,6 8 57 1 63,6 8 119 0,00405731 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, 406855423 Ndufs8 mitochondrial 1 26,4 5 9 1 17 3 5 1 29,7 6 17 1 29,7 6 31 0,00108969 407027854 Capzb F-actin-capping protein subunit beta isoform d 1 19,6 4 4 - 0 0 0 0 0 0 0 0 0 11 0 - 407260963 C1orf101 PREDICTED: uncharacterized protein C1orf101-like 1 6,2 4 4 1 6,4 5 5 0,9995 3,7 2 2 1 8,1 6 11 0,000129 407261678 - PREDICTED: uncharacterized protein LOC77908 1 30,1 3 7 - 0 0 0 1 30,1 3 6 1 30,1 3 13 0,00111949 407263724 - PREDICTED: leucine-rich repeat-containing protein 37A2 1 7,6 18 59 1 7,4 18 40 1 8,9 19 49 1 11,6 26 150 0,000778 41235733 LYRM4 LYR motif-containing protein 4 1 34,1 3 3 1 41,8 4 4 0,9527 11 1 1 1 53,8 5 8 0,000557 disintegrin and metalloproteinase domain-containing protein 5 440546413 Adam5 isoform 1 precursor 1 9,5 6 9 - 1,9 1 2 1 9,1 6 7 1 11,7 8 18 0,00017 45592928 PPAP2A lipid phosphate phosphohydrolase 1 isoform 1 1 13 3 4 1 13 4 9 0,9865 3,5 1 2 1 13 4 15 0,000739 Réplica 1 Réplica 2 Réplica 3 Datos combinados

47059026 SSBP1 single-stranded DNA-binding protein, mitochondrial isoform 1 1 27,7 3 4 - 0 0 0 1 43,9 5 7 1 43,9 5 13 0,000546 471012248 Acr acrosin isoform 3 1 24,2 6 37 1 19,7 5 29 1 32,6 8 46 1 32,6 8 113 0,00318498 47271515 2810408A11Rik protein phosphatase inhibitor 2-like 1 29,2 7 8 - 3,4 1 1 1 18,6 5 6 1 38,2 9 15 0,000368 476007882 Aldoa1b aldolase 1 A retrogene 2 1 42,6 12 13 1 44 12 10 1 42,9 13 19 1 55,5 17 43 0,000762

483968030 Prkaca cAMP-dependent protein kinase catalytic subunit alpha isoform 2 1 38,2 11 15 1 19,8 7 12 1 39,4 13 32 1 44 15 60 0,00128378 49355801 Gykl1 glycerol kinase-like 1 1 27,7 12 23 1 22,6 10 34 1 35,3 15 62 1 35,3 15 119 0,00199479 50582595 CCIN calicin 1 15,6 9 10 1 16,8 7 14 1 38,1 18 48 1 39,5 19 71 0,000907 50582603 Defb22 beta-defensin 22 precursor 1 10,1 3 7 1 10,1 3 14 1 10,1 3 13 1 10,1 3 34 0,00148679 51491845 CLTC clathrin heavy chain 1 1 9,2 9 9 1 3,8 5 5 1 6 6 6 1 12,2 15 20 0,000102 51556221 Ces7 carboxylesterase 5A precursor 1 7,5 3 3 1 8,3 4 6 1 12,3 5 10 1 14,3 6 19 0,00053 51591899 PPP3R2 calcineurin subunit B type 2 1 19 3 5 - 0 0 0 1 19 3 6 1 19 3 11 0,000408 51921291 SLC13A5 solute carrier family 13 member 5 1 5,6 3 3 - 1,9 1 2 1 3,7 3 3 1 5,6 4 8 0,000223 51921341 AA467197 normal mucosa of esophagus-specific gene 1 protein 1 48,2 5 14 1 49,4 6 18 1 49,4 6 20 1 49,4 7 54 0,00540405 succinate dehydrogenase [ubiquinone] flavoprotein subunit, 54607098 SDHA mitochondrial precursor 1 30,1 15 19 1 55 23 124 1 61,9 28 115 1 61,9 27 258 0,00315325 55741460 PARK7 protein DJ-1 1 49,7 7 15 1 42,9 6 11 1 66,1 10 17 1 66,1 10 44 0,0014752 57222276 Adam6b a disintegrin and metalloproteinase domain 6-like 1 12,7 8 10 1 13,9 8 7 1 12,8 7 10 1 17,9 10 28 0,000348

58037109 NDUFB10 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 1 40,3 6 11 1 40,3 6 7 1 26,1 4 7 1 40,3 6 25 0,000795 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, 58037117 Gm12251 mitochondrial precursor 1 34,2 10 30 1 34,2 9 21 1 46 13 46 1 46 14 97 0,00263213 58037257 dcaf7 DDB1- and CUL4-associated factor 7 1 12,9 3 3 1 8,8 3 3 1 10,5 3 5 1 21,6 6 11 0,000297 58037395 SCAMP1 secretory carrier-associated membrane protein 1 1 19,2 5 14 1 24,6 5 24 1 19,2 5 24 1 27,2 7 63 0,00267932 58037413 morn5 MORN repeat-containing protein 5 1 32,4 4 6 - 0 0 0 1 43,5 6 11 1 43,5 6 17 0,000692 58037419 1700018L24Rik testis-expressed sequence 29 protein 1 18,3 3 6 1 18,8 4 7 1 18,8 3 6 1 26,3 5 19 0,000922 58037457 Arpm1 actin-related protein T3 1 15,4 3 3 1 13 4 4 1 27,1 6 8 1 31,7 7 15 0,000364 58037499 LCN12 epididymal-specific lipocalin-12 precursor 1 10,4 2 6 0,9996 5,7 1 3 1 20,7 3 6 1 20,7 3 15 0,00110144 59709445 4930579C15Rik equatorin precursor 1 25,2 5 26 1 24,9 5 19 1 30,3 6 39 1 30,3 6 82 0,00262542 59933292 BC049730 uncharacterized protein LOC232972 precursor 1 7,7 2 5 0,9996 7,7 2 9 1 7,7 2 9 1 7,7 2 23 0,00111118 61316404 Odf3b outer dense fiber protein 3B 1 13,4 3 3 1 22,3 5 7 1 30,3 6 10 1 39,9 8 21 0,00065 61888838 HSD17B10 3-hydroxyacyl-CoA dehydrogenase type-2 1 47,1 7 15 1 47,9 10 14 1 57,1 10 16 1 57,5 11 44 0,00107185 62000656 Fam71b protein FAM71B 1 14 8 24 1 29 13 47 1 24,8 13 60 1 30,2 15 134 0,00224679 62078759 tekt3 tektin-3 1 23,3 10 10 1 16,9 9 25 1 48 22 62 1 48 22 98 0,00131764 65301151 Izumo1 izumo sperm-egg fusion protein 1 precursor 1 28,5 11 69 1 21,7 7 39 1 28,2 10 53 1 33,8 13 161 0,0036192 66392160 VPS13A vacuolar protein sorting-associated protein 13A 1 2,8 8 9 1 6,5 18 24 1 14 33 38 1 15,1 37 70 0,000241 6671509 ACTB actin, cytoplasmic 1 1 51,7 14 38 1 44,5 11 53 1 68,3 17 42 1 68,3 17 88 0,00145454 6671549 Prdx6 peroxiredoxin-6 1 45,1 7 11 1 53,1 9 8 1 54,5 9 10 1 62,5 11 24 0,000607 6671569 Gm9093 60S acidic ribosomal protein P0 1 14,5 4 5 - 0 0 0 0,9992 7,3 2 2 1 14,5 4 7 0,000191 6671678 Car4 carbonic anhydrase 4 precursor 1 26,9 6 17 1 25,2 6 12 1 30,5 7 30 1 33,1 8 57 0,00167332 6671702 cct5 T-complex protein 1 subunit epsilon 1 9,6 5 9 1 6,3 3 6 1 20,5 9 13 1 22 10 27 0,000365 6671746 Cfl2 cofilin-2 1 30,7 4 4 - 0 0 0 1 45,2 6 7 1 45,2 6 11 0,000347 6677905 GLG1 Golgi apparatus protein 1 precursor 1 2,9 3 3 1 2,7 3 3 0 0 0 0 1 4,7 5 6 0,0000357 6678017 slc30a1 zinc transporter 1 1 7,2 3 5 0,9994 4,6 2 3 1 11,1 4 4 1 13,5 5 12 0,000244 6678293 Prss39 inactive serine protease 39 precursor 1 23,7 8 15 1 16,6 6 18 1 34,6 10 25 1 34,6 10 58 0,0018586 6678419 tpp2 tripeptidyl-peptidase 2 1 4,4 5 5 1 23,8 22 26 1 22,8 21 22 1 30,1 28 53 0,000352 6678553 vamp3 vesicle-associated membrane protein 3 1 55,3 5 18 1 55,3 5 8 1 55,3 5 10 1 55,3 5 31 0,0027451 6678908 MNS1 meiosis-specific nuclear structural protein 1 1 7,9 4 6 1 8,8 4 6 1 22 12 20 1 22 12 32 0,000388 high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic 6679249 PDE8A phosphodiesterase 8A 1 9,7 6 6 1 6,8 5 5 1 5,1 3 3 1 15,2 10 14 0,000153 pyruvate dehydrogenase E1 component subunit alpha, testis- 6679263 pdhA2 specific form, mitochondrial precursor 1 50,1 18 98 1 49,9 17 90 1 64,2 21 167 1 62,1 21 354 0,0065772 6679299 1700071K01Rik prohibitin 1 52,2 13 24 1 46,7 12 31 1 62,1 13 36 1 65,4 15 91 0,00211572 6679439 Gm9234 peptidyl-prolyl cis-trans isomerase A 1 29,3 4 5 - 0 0 0 1 36 5 11 1 36 5 16 0,000573 serine/threonine-protein phosphatase 2B catalytic subunit gamma 6679447 PPP3CC isoform 1 17,2 8 9 - 0 0 0 1 13,6 6 12 1 19,1 9 23 0,000425 6679501 psmc1 26S protease regulatory subunit 4 1 13,4 5 5 - 0 0 0 0,9996 4,5 2 2 1 13,4 5 7 0,000106 6679583 RAB11B ras-related protein Rab-11B 1 49,1 9 23 1 43,1 9 23 1 56,4 11 38 1 56,4 11 84 0,00235127 6679939 GAPDHS glyceraldehyde-3-phosphate dehydrogenase, testis-specific 1 54,1 15 69 1 42,7 13 89 1 62,1 17 181 1 62,1 17 336 0,00525809 6680027 glud1 glutamate dehydrogenase 1, mitochondrial precursor 1 10,4 5 5 0,9991 3,8 2 2 1 28,1 13 16 1 30,1 14 22 0,00027 Réplica 1 Réplica 2 Réplica 3 Datos combinados

6753498 COX4I1 cytochrome c oxidase subunit 4 isoform 1, mitochondrial precursor 1 43,8 7 65 1 43,8 7 45 1 39,1 7 54 1 43,8 8 165 0,00599372 6753540 csnk2a2 casein kinase II subunit alpha' 1 49,1 13 19 1 40,6 12 22 1 56,9 16 43 1 62 18 85 0,00195768 6753560 Cyct cytochrome c, testis-specific 1 41,9 5 8 1 47,6 5 9 1 41,9 5 8 1 58,1 7 25 0,00133748 6753618 ddt D-dopachrome decarboxylase 1 60,2 5 5 1 60,2 5 6 1 60,2 5 7 1 79,7 7 19 0,000921 6754000 Gk2 glycerol kinase 2 1 16,6 7 8 1 26,5 11 31 1 38,1 16 75 1 38,1 16 114 0,00170677 6754036 Got2 aspartate aminotransferase, mitochondrial 1 26,7 10 12 1 33,3 12 16 1 32,3 11 21 1 37,4 14 50 0,000888 6754086 GSTM5 glutathione S-transferase Mu 5 1 43,3 9 22 1 51,8 11 44 1 61,6 12 63 1 69,6 15 129 0,00232926 6754254 HSP90AA1 heat shock protein HSP 90-alpha 1 17,9 13 13 1 29,9 17 27 1 23,5 16 20 1 37 22 64 0,000669 6754660 Cd46 membrane cofactor protein precursor 1 9,3 3 5 1 13,2 4 12 1 26,3 6 15 1 29 7 32 0,00101186 6754724 psmd7 26S proteasome non-ATPase regulatory subunit 7 1 12,5 3 4 1 31,2 7 7 1 38 8 8 1 41,1 9 18 0,000474 6755114 Prdx5 peroxiredoxin-5, mitochondrial precursor 1 37,6 8 13 1 45,7 10 15 1 45,7 10 28 1 45,7 10 56 0,00144904 6755196 Psma4 proteasome subunit alpha type-4 1 13,8 4 7 1 16,9 4 6 1 29,1 6 8 1 29,1 6 21 0,000622 6755198 Psma6 proteasome subunit alpha type-6 1 35 7 14 1 57,7 10 15 1 35,8 7 11 1 62,6 11 39 0,00105958 6755202 PSMB3 proteasome subunit beta type-3 1 35,1 5 8 1 18,5 3 4 1 20 3 4 1 35,1 5 18 0,000839 6755204 PSMB5 proteasome subunit beta type-5 1 49,2 9 12 1 58,3 11 13 1 49,2 10 20 1 58,3 12 45 0,00116698 6755210 PSMD13 26S proteasome non-ATPase regulatory subunit 13 1 15,7 6 6 1 7,7 3 3 1 19,7 6 6 1 26,9 9 15 0,000271 6755368 LOC100047329 40S ribosomal protein S18 1 18,4 3 3 - 0 0 0 0,9993 11,2 2 4 1 18,4 3 7 0,000233 6755372 rps3 40S ribosomal protein S3 1 12,3 3 3 1 32,1 6 6 1 23 5 6 1 32,1 6 15 0,000293 6755560 SLC4A1 band 3 anion transport protein 1 9,8 7 11 - 1,5 1 1 1 8,5 7 14 1 13,5 10 26 0,00048 6755614 Spa17 sperm surface protein Sp17 1 23,5 3 11 0,9998 17,4 2 3 1 29,5 4 15 1 40,3 5 30 0,00116963 6755688 STXBP2 syntaxin-binding protein 2 1 7,6 3 3 1 11,3 5 5 1 10,3 4 5 1 16,5 7 15 0,000173 6755863 Hsp90b1 endoplasmin precursor 1 10,1 8 14 1 26,4 19 33 1 15,7 11 21 1 27,9 20 66 0,00058 6755963 VDAC1 voltage-dependent anion-selective channel protein 1 1 26,1 5 7 1 62,5 12 18 1 31,8 6 8 1 65,4 13 33 0,000706 6755965 vdac2 voltage-dependent anion-selective channel protein 2 1 27,8 6 12 1 42,4 10 33 1 52,5 12 36 1 59,7 13 87 0,00186044 67625729 Gm128 protein MENT precursor 1 45,1 10 82 1 45,1 11 66 1 59,4 12 97 1 59,4 13 250 0,009206 67972647 arsA arylsulfatase A precursor 1 12,5 5 7 - 0 0 0 1 18,8 7 10 1 20,4 8 17 0,00056 68226731 npepps puromycin-sensitive aminopeptidase 1 3,4 3 3 0,9994 2,6 2 3 1 7,1 5 5 1 9 7 11 0,000102 6996913 LOC100048867 annexin A2 1 14,2 4 4 1 34,5 9 9 1 22,1 6 6 1 43,7 12 19 0,000324 6996917 G6pdx glucose-6-phosphate 1-dehydrogenase X 1 9,5 4 4 - 0 0 0 1 9,7 5 5 1 14,2 7 9 0,000133 70778976 Pgk1-rs7 phosphoglycerate kinase 1 1 15,1 5 8 - 15,1 5 5 1 17 6 9 1 17 6 25 0,00037 7106277 CSNK2B casein kinase II subunit beta 1 41,4 6 12 1 33,5 5 13 1 41,4 7 14 1 41,4 6 37 0,00183405 7106387 psma5 proteasome subunit alpha type-5 1 22,4 4 7 1 47,7 7 16 1 39,8 7 10 1 48,1 8 33 0,00120357 7110703 PSMC5 26S protease regulatory subunit 8 1 16,5 5 5 1 8,6 3 8 1 21,7 7 8 1 36,5 11 21 0,000295 71774133 ppiB peptidyl-prolyl cis-trans isomerase B precursor 1 22,2 5 6 - 6 1 1 1 46,3 9 14 1 49,5 10 21 0,000571 7242197 psmb1 proteasome subunit beta type-1 precursor 1 39,6 7 12 1 30,8 5 7 1 39,6 7 10 1 46,7 8 28 0,000708 7305229 Ldhc L-lactate dehydrogenase C chain 1 60,8 18 265 1 75 21 252 1 75,6 22 407 1 75 21 928 0,01265867 7305241 lrrc23 leucine-rich repeat-containing protein 23 1 13,5 4 4 0,9993 8,2 2 2 1 23,2 7 13 1 23,2 8 20 0,000438 74315965 Hyal5 hyaluronidase PH-20 1 17,7 9 14 - 0 0 0 1 21,9 10 12 1 21,9 10 26 0,000523 74315975 PSMD1 26S proteasome non-ATPase regulatory subunit 1 1 10,8 9 9 1 17,7 14 17 1 18 14 17 1 23,3 19 43 0,000401 75677532 1700029J07Rik UPF0602 protein C4orf47 homolog 1 22,8 5 8 1 16,1 4 5 1 17,1 4 10 1 33,5 8 22 0,000559 7656914 Atp11a probable phospholipid-transporting ATPase IH 1 3,9 3 3 - 0 0 0 0,9996 1,9 2 2 1 3,9 3 5 0,000036 7657607 SPAG6 sperm-associated antigen 6 1 21,3 9 20 1 15,6 7 15 1 33,1 13 39 1 33,1 13 74 0,00138772

76781472 Gm711 probable inactive protein kinase-like protein SgK071 homolog 1 5,4 3 3 1 11,9 7 7 1 6,9 4 4 1 11,9 7 14 0,000279 76781476 BC089491 selenoprotein V 1 14 3 3 - 4,3 1 1 0,9869 4,3 1 1 1 18,3 4 5 0,000132 7710014 cul3 cullin-3 1 19,5 12 12 1 24,2 16 18 1 24,5 15 15 1 34,1 23 47 0,000365 7710018 stom erythrocyte band 7 integral membrane protein 1 30,3 7 36 1 27,1 6 24 1 32,4 7 23 1 35,2 8 82 0,00209557 78214312 Gm12231 ATP synthase subunit b, mitochondrial precursor 1 22,3 7 13 1 33,2 10 21 1 33,2 10 29 1 33,2 11 62 0,00157568 7949005 LOC674583 ATP synthase-coupling factor 6, mitochondrial precursor 1 35,2 3 3 0,9995 26,9 2 2 0,9994 25,9 2 2 1 35,2 3 7 0,000362 82889968 DCST2 PREDICTED: DC-STAMP domain-containing protein 2 1 4,7 3 4 - 1,3 1 1 0,9999 4,4 3 3 1 6,6 4 8 0,000108 82904142 Gm1096 PREDICTED: CD177 antigen isoform 2 1 11,2 2 3 - 5,6 1 1 0,9996 5,6 1 2 1 11,2 2 6 0,000214 83627689 SLC22A14 solute carrier family 22 member 14 1 5,1 3 4 - 0 0 0 0,9871 2,4 1 1 1 6,7 4 6 0,000139 83715998 Gm2972 ATP synthase subunit e, mitochondrial 1 50,7 3 7 1 60,6 4 6 1 60,6 4 8 1 60,6 4 21 0,00161677 83816907 Gm15785 dnaJ homolog subfamily B member 6 isoform a 1 21,9 7 11 1 11,2 4 13 1 28,2 9 16 1 28,2 9 35 0,000892 Réplica 1 Réplica 2 Réplica 3 Datos combinados

Probabilidad Números de Porcentaje Números de Porcentaje Números de Probabilidad Porcentaje Referencia de Porcentaje de péptidos Número de Probabilidad de péptidos Número de Probabilidad de péptidos Número de de de Números de Número de GI Proteína Nombre de la proteína coincidencia cobertura únicos espectros de coincidencia cobertura únicos espectros de coincidencia cobertura únicos espectros coincidencia cobertura péptidos únicos espectros Valor APEX putative ATP-dependent Clp protease proteolytic subunit, 8393156 clpP mitochondrial precursor 1 18 4 12 1 28,7 6 11 1 28,7 6 17 1 28,7 6 39 0,0012312 serine/threonine-protein phosphatase 2A catalytic subunit beta 8394024 PPP2CB isoform 1 17,5 4 4 1 16,5 3 3 1 30,7 7 8 1 35,3 8 15 0,000375 84490394 Spnb1 spectrin beta chain, erythrocytic 1 2,6 5 8 1 3,3 5 6 1 5,4 10 12 1 7,7 14 26 0,00008 84794552 PEBP1 phosphatidylethanolamine-binding protein 1 1 58,3 7 36 1 82,9 10 23 1 82,9 10 42 1 82,9 10 101 0,00556509 84871978 MYCBP C-Myc-binding protein 1 36,9 3 6 1 56,3 5 6 1 64,1 5 10 1 65 6 23 0,00138195 84993736 acsbg2 long-chain-fatty-acid--CoA ligase ACSBG2 1 20,5 10 19 1 21,1 10 34 1 25,2 12 41 1 27,9 14 92 0,00130226 85662408 Crat carnitine O-acetyltransferase 1 28,4 16 108 1 26,4 15 91 1 42,7 19 135 1 42,7 19 341 0,0048572 85701842 Gm216 uncharacterized protein C2orf62 homolog 1 7,8 3 3 - 0 0 0 0,9687 1,8 1 1 1 9,6 4 5 0,000118 85701985 Gm1060 dynein regulatory complex protein 1 1 4,9 4 5 1 13,3 9 11 1 13 9 14 1 15,4 11 30 0,000314 85702045 LOC100048051 multidrug and toxin extrusion protein 2 1 6,3 3 3 0,9997 3,8 2 3 1 6,3 3 4 1 6,3 3 10 0,000295 85702063 1700071K01Rik prohibitin-like 1 35,4 9 3 1 38,7 9 8 1 44,3 9 9 1 47,6 11 20 0,000496 87239970 lipe hormone-sensitive lipase isoform 1 1 3,2 3 3 1 9,9 6 9 1 10,4 7 15 1 15 10 28 0,000336 88196767 MLF1 myeloid leukemia factor 1 isoform a 1 23,2 6 7 1 22,1 4 7 1 34,8 7 9 1 38,6 8 23 0,000629 89886473 9030607L17Rik izumo sperm-egg fusion protein 4 precursor 1 34,8 8 55 1 34,8 8 20 1 37,9 9 51 1 37,9 9 125 0,00377025 90093343 1700052N19Rik UPF0364 protein C6orf211 homolog 1 8,9 4 4 - 0 0 0 0 0 0 0 1 8,9 4 4 0,0000993 phospholipid hydroperoxide glutathione peroxidase, nuclear 90903233 Gpx4 isoform 1 1 22,3 4 10 1 27,4 5 17 1 48,7 10 43 1 48,7 10 70 0,00149395 9256624 PGAM2 phosphoglycerate mutase 2 1 47 12 58 1 46,2 12 52 1 49 16 58 1 54,2 16 164 0,00483985 93102411 GALC galactocerebrosidase precursor 1 5,1 3 3 1 8 3 4 0,9993 5,1 2 2 1 13,6 6 10 0,000207 94536844 rpiA ribose-5-phosphate isomerase 1 18,5 4 4 0,9993 10,6 2 3 0,9997 6,9 2 2 1 18,5 4 9 0,00022 9506589 Fbp1 fructose-1,6-bisphosphatase 1 1 29,6 7 14 1 47 11 15 1 53,3 13 22 1 53,3 13 51 0,00126818 9506739 nagK N-acetyl-D-glucosamine kinase isoform 1 1 22,7 6 8 1 29,7 7 11 1 28,3 7 9 1 35,3 9 27 0,000732 95772156 1700019N12Rik testis-expressed sequence 40 protein 1 21,6 4 10 1 21,6 4 5 0,9837 7,7 1 2 1 21,6 4 17 0,000785 9789881 Arl3 ADP-ribosylation factor-like protein 3 1 23,6 3 3 1 23,6 3 3 1 38,5 5 7 1 38,5 5 14 0,000522 9789907 STUB1 STIP1 homology and U box-containing protein 1 1 15,8 4 4 0,9992 7,2 2 2 1 10,9 3 3 1 19,4 5 9 0,000164 LETM1 and EF-hand domain-containing protein 1, mitochondrial 9789997 LETM1 precursor 1 6,2 4 4 1 23,7 13 18 1 33,7 17 23 1 31,7 18 44 0,000459 9790051 pfkp 6-phosphofructokinase type C 1 36,1 19 31 1 32,7 21 45 1 42,6 25 50 1 45,3 29 128 0,00156463 9790055 Gm2199 mitochondrial carrier homolog 2 1 13,2 4 5 1 22,1 5 11 1 36,6 8 23 1 36,6 8 40 0,00131991 9790131 Slc22a21 solute carrier family 22 member 21 1 5,7 3 5 1 5,3 2 6 1 5,7 3 5 1 11 5 16 0,000477

9910194 dhodh dihydroorotate dehydrogenase (quinone), mitochondrial precursor 1 10,4 4 4 - 0 0 0 0,9996 4,8 2 2 1 10,4 4 6 0,000124 9910582 Tex101 testis-expressed sequence 101 protein precursor 1 30,4 6 11 1 32,4 6 16 1 30,4 6 19 1 36 7 46 0,00198243 194353962 Gm8230 ADP-ribosylation factor 1 0,9999 29,3 4 7 - 0 0 0 0,9828 53,6 7 8 0,9828 53,6 7 12 - 282398110 Krt82 keratin, type II cuticular Hb2 0,9999 5,8 3 3 - 0 0 0 0 0 0 0 0,9999 5,8 3 3 0,0000465

84781779 NDUFB6 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 0,9999 19,5 2 3 - 0 0 0 1 25 3 7 1 25 3 10 0,000556 111955131 Gm4787 a disintegrin and metalloprotease domain 4b precursor 0,9998 7,9 5 2 1 14,2 9 15 1 9,2 6 3 1 16 10 34 0,000408 113931164 Fndc8 fibronectin type III domain-containing protein 8 0,9998 8,7 2 4 1 19 4 6 1 42,4 9 27 1 41,1 8 36 0,00102275

114326522 Cd55 complement decay-accelerating factor, GPI-anchored precursor 0,9998 7,7 2 4 - 3,1 1 1 1 12,3 4 7 1 12,3 4 12 0,000343 116812883 Dcst1 DC-STAMP domain-containing protein 1 0,9998 2,7 2 2 - 0 0 0 1 4,6 3 3 1 4,6 3 5 0,0000621 13386062 APOOL apolipoprotein O-like precursor 0,9998 10,9 2 2 1 37,7 8 9 1 27,9 6 6 1 43,8 9 17 0,000392 146262012 DDI1 protein DDI1 homolog 1 0,9998 7,6 2 2 - 4,2 1 2 0,9998 7,6 2 3 0,9998 7,6 2 7 0,000199 148747526 Rab7 ras-related protein Rab-7a 0,9998 13 2 2 0,9995 10,1 2 2 0,969 6,8 1 1 1 23,2 4 5 0,000123 160298209 GOT1 aspartate aminotransferase, cytoplasmic 0,9998 6,5 2 2 - 0 0 0 1 14,3 5 5 1 17,4 6 7 0,00011 213688406 Gm11582 dynein light chain 1, cytoplasmic 0,9998 50,6 4 4 1 58,4 4 13 1 50,6 4 7 1 59,6 5 23 0,00205824 21489937 lancl1 lanC-like protein 1 0,9998 5,8 2 2 1 12,8 4 4 1 16,8 5 7 1 20,3 6 13 0,000319 226874906 YWHAE 14-3-3 protein epsilon 0,9998 12,9 3 2 1 24,3 5 6 1 22,7 5 4 1 37,6 8 13 0,000302 242397497 1700012P22Rik uncharacterized protein C1orf158 homolog 0,9998 10,2 2 2 1 40,3 8 9 1 31,6 5 8 1 51 9 19 0,000701 27370404 CCDC113 coiled-coil domain-containing protein 113 0,9998 6,4 2 2 0,9996 6,1 2 4 0,9989 7,7 2 4 1 12,7 4 9 0,000158 27754103 Psmc6 26S protease regulatory subunit 10B 0,9998 6,7 2 2 - 2,3 1 1 1 14,7 5 5 1 14,7 5 8 0,000134 28316750 HIST1H2BA histone H2B type 1-A 0,9998 33,9 4 2 - 0 0 0 0,9998 27,6 3 2 0,9998 39,4 5 4 0,00019 30425270 FAM78A protein FAM78A 0,9998 9,9 2 2 0,9996 11 2 3 1 18 4 4 1 18 4 9 0,000363 309264486 fsip2 PREDICTED: fibrous sheath-interacting protein 2 0,9998 0,4 2 2 1 6,1 33 77 1 6,4 36 58 1 9,1 50 141 0,00024 31541988 CCDC38 coiled-coil domain-containing protein 38 0,9998 4,3 2 4 - 0 0 0 0 0 0 0 0,9998 4,3 2 4 0,0000494 40556608 HSP90AB1 heat shock protein HSP 90-beta 0,9998 10,8 7 2 1 17,3 10 7 1 15,3 10 3 1 22,1 13 12 0,000117 PREDICTED: transmembrane emp24 domain-containing protein 2- 407263560 Tmed9 like 0,9998 10,9 2 2 - 0 0 0 0,9997 10,9 2 2 0,9998 10,9 2 4 0,000202 6754084 Gm5562 glutathione S-transferase Mu 1 0,9998 26,1 5 2 1 27,5 6 7 1 32,6 6 7 1 44 9 16 0,000414 67846103 SHMT1 serine hydroxymethyltransferase, cytosolic 0,9998 4,4 2 2 - 1,9 1 1 1 12,1 5 5 1 14,6 6 8 0,000134 76253942 cetn1 centrin-1 0,9998 17,4 2 4 - 0 0 0 1 27,9 4 5 1 27,9 4 9 0,000349

94366539 Gm13570 PREDICTED: mitochondrial pyruvate carrier-like protein-like 0,9998 42,6 4 6 - 0 0 0 0 0 0 0 0,9999 61,5 5 16 0,000977 119508443 DNAJC10 dnaJ homolog subfamily C member 10 precursor 0,9997 3,4 2 2 1 7,4 5 6 1 4,9 3 3 1 11,1 7 11 0,00012 125988403 SPCS3 signal peptidase complex subunit 3 0,9997 11,7 2 2 - 0 0 0 1 17,8 3 3 1 17,8 3 5 0,000216 12963697 Fahd1 acylpyruvase FAHD1, mitochondrial 0,9997 7 1 2 1 26,9 5 8 1 34,4 5 7 1 38,3 6 17 0,000604 Réplica 1 Réplica 2 Réplica 3 Datos combinados

146134392 1110020G09Rik NAD kinase domain-containing protein 1, mitochondrial isoform 1 0,9997 6,7 2 2 - 0 0 0 0,9991 9,5 2 2 1 10 3 3 0,0000456 19526481 Spna1 spectrin alpha chain, erythrocytic 1 0,9997 0,8 2 2 1 3,3 6 7 1 3,1 5 5 1 6,3 11 14 0,0000412 19526900 TMEM30A cell cycle control protein 50A 0,9997 9,1 2 2 - 0 0 0 0,9998 9,1 2 2 0,9998 9,1 2 4 0,000117 19745150 cyb5r3 NADH-cytochrome b5 reductase 3 0,9997 7 2 2 0,9998 10,3 2 2 0,9994 6,3 2 2 1 16,6 4 6 0,000153 21312712 TCEB1 transcription elongation factor B polypeptide 1 0,9997 18 1 2 - 18 1 1 0,9711 9 1 1 1 27 2 4 0,000275 21312794 Pfn3 profilin-3 0,9997 19,7 2 2 1 54,7 7 9 1 54,7 7 9 1 61,3 8 20 0,00086 225703118 Ado 2-aminoethanethiol dioxygenase 0,9997 7,8 2 2 1 24,2 5 6 1 23,4 5 5 1 27 6 13 0,000432 254587947 ppp2r4 serine/threonine-protein phosphatase 2A activator 0,9997 7,1 2 2 1 26,9 7 7 1 10,2 3 4 1 26,9 7 13 0,0003 254826802 4930511I11Rik armadillo repeat-containing protein 12 0,9997 7,1 2 3 1 19,4 5 8 1 12,4 3 6 1 19,4 5 17 0,000555 34304087 SPINK2 serine protease inhibitor Kazal-type 2 precursor 0,9997 16,3 1 2 1 27,9 2 3 0,9832 16,3 1 1 1 27,9 2 6 0,000512 377833423 C8orfk29 PREDICTED: putative transmembrane protein C8orfK29 0,9997 10,6 2 2 0,9995 7,8 2 2 0,9737 4,5 1 1 1 13,9 3 5 0,000154 47059013 KRT73 keratin, type II cytoskeletal 73 0,9997 6,5 3 19 0,9994 4,3 2 9 0 0 0 0 0,9994 6,5 3 16 0,000215 54020730 EFR3A protein EFR3 homolog A 0,9997 3,1 2 3 1 11,8 8 10 1 9,3 6 9 1 14,8 10 24 0,000245 58037161 LYZL6 lysozyme-like protein 6 precursor 0,9997 12,8 1 4 0,9994 12,8 1 2 1 18,9 2 7 1 18,9 2 13 0,00148576 58037189 1700026L06Rik uncharacterized protein C9orf9 homolog 0,9997 17,9 2 6 1 30,4 3 12 1 30,4 3 17 1 30,4 3 35 0,00181033 66392180 Pacrg parkin coregulated gene protein homolog 0,9997 9,5 2 4 0,9995 9,5 2 3 1 9,5 2 9 1 9,5 2 16 0,000493 6755901 Gm7172 tubulin alpha-1A chain 0,9997 56,8 21 17 - 0 0 0 0,9986 65,4 23 7 0,9989 65,4 23 14 0,00018 G protein-activated inward rectifier potassium channel 2 isoform 71051587 Kcnj6 Girk2A-1 0,9997 6 2 2 - 0 0 0 0,9998 6 2 2 0,9998 6 2 4 0,000108 85701762 LOC100046792 uncharacterized protein C6orf163 homolog 0,9997 7 2 2 - 0 0 0 1 17,7 5 5 1 17,7 5 7 0,000133 91206446 Gm14316 serine protease inhibitor, Kunitz type 5 precursor 0,9997 11,4 1 2 1 28,6 3 4 0,9801 11,4 1 1 1 28,6 3 7 0,000454 94721328 vapa vesicle-associated membrane protein-associated protein A 0,9997 8 2 2 1 18,1 3 5 1 21,3 4 7 1 21,3 4 16 0,000576

9506911 NDUFA1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 0,9997 14,3 1 2 0,9997 14,3 1 2 0,9992 14,3 1 2 0,9997 14,3 1 6 0,000557 13384642 synj2bp synaptojanin-2-binding protein 0,9996 15,9 2 2 - 0 0 0 1 24,1 3 4 1 24,1 3 6 0,000338 13385384 PSMD12 26S proteasome non-ATPase regulatory subunit 12 0,9996 3,9 2 2 - 0 0 0 1 12,9 6 7 1 12,9 6 9 0,000137 154090941 Krt42 keratin, type I cytoskeletal 42 0,9996 8,2 4 2 - 0 0 0 0,9828 6,2 3 1 0,9998 8,2 4 3 0,0000581 158631221 1700093K21Rik uncharacterized protein C2orf74 homolog 0,9996 12,2 2 5 1 18,4 3 3 1 18,4 3 7 1 24,5 5 16 0,000815 18087731 Dynll2 dynein light chain 2, cytoplasmic 0,9996 58,4 5 3 1 49,4 3 10 1 50,6 4 7 1 58,4 5 23 0,00204365 18087805 Gm9168 40S ribosomal protein S2 0,9996 6,1 2 2 - 0 0 0 0 0 0 0 0,9996 6,1 2 2 0,0000403 19482172 rdh11 retinol dehydrogenase 11 precursor 0,9996 7 2 2 0,999 5,1 2 2 1 11,1 3 3 1 13,6 4 7 0,00019 20911031 KRT5 keratin, type II cytoskeletal 5 0,9996 7,2 4 11 - 0 0 0 0,9845 5,7 3 1 0,9844 7,2 4 2 - 21314830 REEP6 receptor expression-enhancing protein 6 isoform 1 0,9996 13,2 2 4 - 7,5 1 1 1 32,8 5 10 1 32,8 5 16 0,00104105 227116292 DPP4 dipeptidyl peptidase 4 isoform 2 0,9996 3,3 2 2 1 4,8 3 3 0 0 0 0 1 6,2 4 5 0,0000579

23956130 ptpmt1 phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1 0,9996 7,3 2 3 - 0 0 0 1 32,6 7 10 1 32,6 7 13 0,00033 28201956 RAE1 mRNA export factor 0,9996 6,5 2 2 - 0 0 0 0,98 2,4 1 1 0,9998 6,5 2 3 0,0000835 282721029 CTBP2 C-terminal-binding protein 2 isoform 1 0,9996 4,3 2 2 - 0 0 0 0,9992 4,3 2 2 0,9997 4,3 2 4 0,0000435 PREDICTED: up-regulated during skeletal muscle growth protein 5- 309265053 - like 0,9996 27,6 2 3 1 44,8 4 6 0,9867 25,9 1 3 1 44,8 4 12 0,00158273 31543976 YWHAG 14-3-3 protein gamma 0,9996 13,8 3 2 0,9996 9,7 2 2 0,9996 13,8 3 2 0,9997 13,8 3 6 0,000163 31981690 LOC641192 heat shock cognate 71 kDa protein 0,9996 14,1 8 7 1 28,9 14 25 1 19 10 21 1 30,3 15 54 0,000627 40254507 PGP phosphoglycolate phosphatase 0,9996 8,4 2 2 1 16,8 5 5 1 19,3 5 5 1 29 8 12 0,000264 42415475 p4hb protein disulfide-isomerase precursor 0,9996 5,1 2 2 - 0 0 0 1 13 6 6 1 10,6 5 7 0,0000818 NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, 56711244 NDUFS6 mitochondrial precursor 0,9996 31,9 2 2 1 51,7 4 5 1 51,7 4 5 1 51,7 4 12 0,000584 6679066 NIPSNAP1 protein NipSnap homolog 1 0,9996 8,1 2 2 1 15,8 4 4 0 0 0 0 1 15,8 4 6 0,000167 pyruvate dehydrogenase E1 component subunit alpha, somatic 6679261 Pdha1 form, mitochondrial precursor 0,9996 2,6 1 2 0,9985 2,6 1 2 1 11 4 11 1 11 4 15 0,000252 6753520 CREG1 protein CREG1 precursor 0,9996 13,2 2 3 - 8,2 1 1 1 16,4 3 4 1 16,4 3 8 0,000319 6754696 Gm16379 macrophage migration inhibitory factor 0,9996 17,4 2 2 0,9997 17,4 2 2 0,9993 15,7 2 2 1 23,5 3 6 0,000691 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial 7949037 ech1 precursor 0,9996 7,6 2 2 1 15,3 4 6 1 16,8 4 5 1 27,2 7 13 0,000279 85861182 aldh1a1 retinal dehydrogenase 1 0,9996 6,4 2 2 - 0 0 0 0,9693 2,2 1 1 1 8,6 3 3 0,0000474 110625796 4931432M23Rik inactive serine protease 54 precursor 0,9995 6,5 2 2 - 0 0 0 1 15,4 5 7 1 15,4 6 10 0,000317 13277394 GRPEL1 grpE protein homolog 1, mitochondrial precursor 0,9995 8,8 2 4 1 37,8 7 7 1 20,7 4 6 1 37,8 7 17 0,000539 13384756 EEF1E1 eukaryotic translation elongation factor 1 epsilon-1 0,9995 9,8 2 2 - 0 0 0 1 9,8 2 3 1 9,8 2 5 0,000225 13430890 Hist1h1e histone H1.4 0,9995 10,5 2 3 - 0 0 0 0 0 0 0 1 14,6 3 4 0,000161 157426871 TMC5 transmembrane channel-like protein 5 isoform a 0,9995 4 2 2 - 0 0 0 0 0 0 0 0,9995 4 2 2 0,0000264 160298213 LOC630729 glutathione reductase, mitochondrial precursor 0,9995 6,4 2 2 - 0 0 0 1 20,4 7 7 1 20,4 7 9 0,000172 16716569 PRSS1 protease, serine, 1 precursor 0,9995 8,1 1 9 0,9995 8,1 1 13 0,9997 8,1 1 15 0,9997 8,1 1 40 0,00210094 170650609 INPP1 inositol polyphosphate 1-phosphatase 0,9995 7,1 2 2 - 0 0 0 1 10,9 3 3 1 10,9 3 5 0,000116 17933768 grhpr glyoxylate reductase/hydroxypyruvate reductase 0,9995 9,1 2 2 0,9993 9,1 2 2 0,9823 5,5 1 1 0,9997 9,1 2 5 0,000143 214010153 APEH acylamino-acid-releasing enzyme 0,9995 4 2 2 - 1,6 1 1 1 10,5 4 4 1 12 5 8 0,000114 21426853 TCTEX1D2 tctex1 domain-containing protein 2 0,9995 13,9 2 3 - 7,6 1 1 0,9836 7,6 1 1 0,9995 13,9 2 5 0,000298 Réplica 1 Réplica 2 Réplica 3 Datos combinados

254675160 4921530D09Rik uncharacterized coiled-coil domain-containing protein KIAA1984 0,9995 4,3 2 3 1 12,2 6 12 1 25,1 12 28 1 25,1 12 44 0,00054 254939666 CCDC37 coiled-coil domain-containing protein 37 0,9995 3,6 2 2 - 1,8 1 5 0 0 0 0 0,9995 3,6 2 7 0,0000901 318043086 - uncharacterized protein LOC73863 0,9995 25,2 2 3 1 32,8 3 3 1 25,2 2 3 1 32,8 3 9 0,000535 40804746 TEPP testis, prostate and placenta-expressed protein isoform 1 0,9995 17,3 2 2 1 58,3 11 21 1 63,9 13 26 1 61,6 12 47 0,00173952 46049022 PSMD6 26S proteasome non-ATPase regulatory subunit 6 0,9995 6,7 2 2 - 0 0 0 1 13,1 4 4 1 13,4 4 5 0,0000803 58037149 2200002J24Rik uncharacterized protein LOC69147 0,9995 21,8 2 3 0,9994 21,8 2 3 0 0 0 0 1 21,8 2 6 0,000513 6679811 FLOT2 flotillin-2 isoform 2 0,9995 5,3 2 2 - 0 0 0 0 0 0 0 0,9995 5,3 2 2 0,0000283 6680712 aqp7 aquaporin-7 0,9995 8,6 2 4 - 5,3 1 2 0,9994 8,6 2 3 0,9995 8,6 2 9 0,000549 7305185 INSL6 insulin-like peptide INSL6 precursor 0,9995 8,9 2 2 1 24,6 4 5 1 18,3 3 4 1 24,6 4 11 0,000461 75992911 Acsl6 long-chain-fatty-acid--CoA ligase 6 isoform 1 0,9995 3,2 2 2 1 13,9 8 8 1 13,1 6 6 1 23,1 12 16 0,000182 86198316 Pdia4 protein disulfide-isomerase A4 precursor 0,9995 3,9 2 2 1 25,4 12 14 1 4,5 3 3 1 26,5 13 19 0,00021 114326546 pgam1 phosphoglycerate mutase 1 0,9994 22 5 2 1 30,3 6 4 1 37,8 8 4 1 44,1 9 10 0,000307 124486895 PGD 6-phosphogluconate dehydrogenase, decarboxylating 0,9994 4,6 2 2 - 0 0 0 1 16,6 7 7 1 16,6 7 9 0,000145 12963569 Ppp1r7 protein phosphatase 1 regulatory subunit 7 0,9994 5,8 2 2 - 0 0 0 0,9543 2,8 1 2 0,9994 5,8 2 4 0,0000786 13386040 atp5s ATP synthase subunit s, mitochondrial precursor 0,9994 9,5 2 3 1 39 6 7 1 35 5 11 1 39,5 7 21 0,000653 disintegrin and metalloproteinase domain-containing protein 24 157012013 Adam24 precursor 0,9994 2,8 2 2 1 7,4 4 5 0,9994 2,8 2 2 1 10,2 6 10 0,000174 160415213 RHOC rho-related GTP-binding protein RhoC precursor 0,9994 14,5 2 3 - 0 0 0 0 0 0 0 0 0 4 0 - 169808427 fucA1 tissue alpha-L-fucosidase precursor 0,9994 4,2 2 2 - 0 0 0 1 6,9 3 3 1 8,6 4 5 0,000135 21919408 ODF4 outer dense fiber protein 4 0,9994 7,2 2 2 - 4,1 1 1 0 0 0 0 0,9996 7,2 2 3 0,000161 iron-sulfur cluster co-chaperone protein HscB, mitochondrial 225703114 hscB precursor 0,9994 12 2 3 1 23,1 4 7 1 32,9 6 10 1 32,9 6 22 0,000555 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 226437665 NDUFA11 11 0,9994 23,8 2 3 - 0 0 0 0,9995 23,8 2 3 1 39,2 3 8 0,000615 238637277 SLC3A2 4F2 cell-surface antigen heavy chain isoform a 0,9994 4,2 2 2 0,9995 5,3 2 3 0,9993 4 2 2 1 10,8 5 7 0,000114 24497431 nupl1 nucleoporin p58/p45 0,9994 4,6 2 2 - 0 0 0 0,9747 2 1 1 1 6,6 3 3 0,000115 260064069 WDR65 WD repeat-containing protein 65 0,9994 1,5 2 2 1 19,7 19 34 1 34,3 29 48 1 36,2 31 83 0,000488

298676450 Carkd ATP-dependent (S)-NAD(P)H-hydrate dehydratase isoform 1 0,9994 11 2 2 0,9993 6,7 2 2 0,9996 10,7 2 3 1 17,4 4 8 0,000176 309262133 Akd1 PREDICTED: adenylate kinase domain-containing protein 1 0,9994 1,1 2 3 1 8,1 13 18 1 5,4 9 10 1 9,6 16 32 0,000144 6753036 ALDH2 aldehyde dehydrogenase, mitochondrial precursor 0,9994 5 2 3 1 9,1 4 6 1 31 12 13 1 31 12 22 0,000336 9790123 NME4 nucleoside diphosphate kinase, mitochondrial precursor 0,9994 11,8 2 2 - 0 0 0 1 30,6 5 5 1 30,6 5 7 0,00038 118130874 1700001P01Rik uncharacterized protein C17orf98 homolog 0,9993 10,3 2 2 - 4,5 1 1 1 50,3 5 6 1 50,3 5 9 0,000432 118150478 #N/A coiled-coil domain-containing protein 173 0,9993 3,8 2 2 0,9984 3,3 2 2 1 14,4 6 10 1 16,1 7 16 0,000175 13385518 Arl8b ADP-ribosylation factor-like protein 8B 0,9993 8,6 2 3 - 0 0 0 1 38,7 6 8 1 38,7 6 12 0,000422 169646320 DPY19L1 probable C-mannosyltransferase DPY19L1 0,9993 3,5 2 2 - 0 0 0 0 0 0 0 0,9994 3,5 2 2 0,0000355 194097451 CATSPER4 cation channel sperm-associated protein 4 isoform 2 0,9993 6,8 2 2 0,9997 5,4 2 4 1 9,5 3 3 1 12,5 4 9 0,000262 19526848 MOSC2 MOSC domain-containing protein 2, mitochondrial precursor 0,9993 6,5 2 2 0,9997 6,5 2 2 0,9984 6,5 2 2 0,9997 6,5 2 6 0,000131 21312276 IMMP1L mitochondrial inner membrane protease subunit 1 0,9993 18,1 2 3 - 0 0 0 1 31,3 4 4 1 31,3 4 7 0,000301 21312532 tmem38b trimeric intracellular cation channel type B 0,9993 8,6 2 2 - 5,1 1 1 0,9991 7,5 2 2 1 17,8 4 5 0,000172 227116345 psmb2 proteasome subunit beta type-2 0,9993 9,5 2 2 1 16,4 3 4 1 32,3 5 10 1 32,3 5 16 0,000642 succinate dehydrogenase [ubiquinone] cytochrome b small subunit, 27229021 sdhD mitochondrial 0,9993 10,7 2 2 - 6,3 1 1 0,9871 6,3 1 1 0,9994 10,7 2 4 0,000341 31980806 PGRMC1 membrane-associated progesterone receptor component 1 0,9993 8,7 2 3 1 21,5 3 5 1 22,1 4 5 1 26,7 5 13 0,000453 31981830 COX7A2 cytochrome c oxidase subunit 7A2, mitochondrial precursor 0,9993 27,7 2 3 1 27,7 2 5 1 27,7 2 4 1 27,7 2 12 0,00113843 31982437 nsdhl sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating 0,9993 8,3 2 2 1 19,1 5 6 1 11,3 3 3 1 22,7 6 11 0,000312

58037235 OXSM 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial precursor 0,9993 4,8 2 2 - 3,1 1 1 1 32,5 9 11 1 27,2 8 13 0,000247 6677983 clpB caseinolytic peptidase B protein homolog 0,9993 4 2 2 - 0 0 0 1 5,8 3 4 1 8,1 4 6 0,0000719 6679863 FXN frataxin, mitochondrial precursor 0,9993 11,1 2 2 - 5,3 1 1 1 25,1 4 4 1 16,9 3 6 0,000208 6680724 Arf6 ADP-ribosylation factor 6 0,9993 12,6 2 2 - 0 0 0 1 43,4 5 7 1 33,1 4 7 0,000317 6755536 slc16a7 monocarboxylate transporter 2 0,9993 3,9 2 4 - 1,9 1 3 1 3,9 2 5 1 3,9 2 12 0,000644 6806917 gm2a ganglioside GM2 activator precursor 0,9993 10,4 2 2 0,9997 10,4 2 2 1 21,8 3 3 1 21,8 3 7 0,000368 71725343 LOC632398 malonyl-CoA-acyl carrier protein transacylase, mitochondrial 0,9993 7,3 2 2 1 13,6 4 4 1 18,4 6 6 1 26,5 8 12 0,000287 113931146 1700019L03Rik uncharacterized protein C9orf117 homolog 0,9992 4,6 2 2 0,9991 3,6 2 2 0,9995 4,4 2 2 1 9,4 4 5 0,000076 12963591 Stoml2 stomatin-like protein 2, mitochondrial 0,9992 5,7 2 2 1 18,4 5 5 1 22,9 6 6 1 26,1 7 13 0,000266 12963609 Qsox1 sulfhydryl oxidase 1 isoform b precursor 0,9992 4,2 2 2 - 0 0 0 0 0 0 0 0,9992 4,2 2 2 0,0000283 147904547 CHMP6 charged multivesicular body protein 6 0,9992 10 2 2 - 6,5 1 1 0 0 0 0 0,9993 10 2 3 0,000144 156713423 PSMD8 26S proteasome non-ATPase regulatory subunit 8 0,9992 4,5 2 2 1 11,9 4 4 1 17,8 4 4 1 20,7 5 10 0,000248 242247213 FAM183B protein FAM183B isoform 1 0,9992 29,9 2 2 - 0 0 0 0,9996 29,9 2 3 0,9995 29,9 2 5 0,000438 27734998 Tmem11 transmembrane protein 11, mitochondrial isoform 1 0,9992 13,6 2 2 - 0 0 0 1 27,3 5 7 1 27,3 5 9 0,000467 293597569 Aldoa2 fructose-bisphosphate aldolase A isoform 2 0,9992 63,7 15 16 0,9999 67,6 19 22 1 82,1 23 46 1 82,1 25 82 0,00100089 294610650 Gstt4 glutathione S-transferase theta-4 0,9992 10 2 2 0,9994 10 2 3 0,9995 9,2 2 3 1 12,9 3 9 0,000282 31560378 Tspan6 tetraspanin-6 0,9992 8,2 2 2 0,9995 8,6 2 3 0,9869 4,9 1 2 1 11,8 3 7 0,000296 58037521 CST11 cystatin-11 precursor 0,9992 14,4 2 2 - 0 0 0 0,9858 8,6 1 1 0,9995 14,4 2 3 0,000133 Réplica 1 Réplica 2 Réplica 3 Datos combinados

13385054 NDUFB3 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 0,9991 17,3 2 3 0,9994 17,3 2 3 1 33,7 3 4 1 33,7 3 10 0,000678 183074523 Gm3448 t-complex-associated testis expressed 3-like 0,9991 9,4 2 6 - 5,2 1 1 0,9994 9,4 2 7 0,9995 9,4 2 14 0,000518 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, 225903452 Gm9050 mitochondrial-like 0,9991 27,3 2 2 - 11,3 1 2 1 27,3 2 11 1 27,3 2 15 0,000972 hydroxyacylglutathione hydrolase, mitochondrial isoform 1 227499238 HAGH precursor 0,9991 4,6 1 3 1 20,7 5 9 1 17,7 4 5 1 23 6 16 0,000419 31982332 GLUL glutamine synthetase 0,9991 4,6 2 2 - 2,7 1 4 1 23,9 8 21 1 23,9 8 27 0,000648 6680836 CALR calreticulin precursor 0,9991 6 2 2 - 3,1 1 1 1 14,4 5 7 1 10,8 4 9 0,0002 168229259 MYCBPAP MYCBP-associated protein 0,999 2,1 2 2 - 0 0 0 0,9856 1,1 1 1 0,9994 2,1 2 3 0,0000363 188219602 INPP5A inositol polyphosphate-5-phosphatase A isoform b 0,999 4,6 2 2 - 0 0 0 1 8,7 4 4 1 8,7 4 6 0,000104 small glutamine-rich tetratricopeptide repeat-containing protein 21313588 Sgta alpha 0,999 7,9 2 2 1 12,1 3 3 0,9998 6,7 2 3 1 14,9 4 8 0,000235 21536248 GSTT3 glutathione S-transferase, theta 3 0,999 13,3 2 2 1 19,5 3 3 0,9995 13,3 2 3 1 19,5 3 8 0,000297 21728376 Gm6242 myosin regulatory light chain 12B 0,999 12,2 2 2 - 0 0 0 0,9848 6,4 1 1 0,9997 12,2 2 3 0,000123 22203753 PPA2 inorganic pyrophosphatase 2, mitochondrial precursor 0,999 6,4 2 2 1 11,8 3 4 1 10,3 3 4 1 16,7 5 10 0,000204 227500514 1700080E11Rik uncharacterized protein LOC73532 0,999 12,6 2 2 - 5,3 1 1 1 18,4 3 5 1 23,3 4 8 0,000253 229577224 RAB17 ras-related protein Rab-17 0,999 6,1 1 4 - 6,1 1 1 0,9781 6,1 1 1 0,9991 6,1 1 7 0,000257 238550131 Efcab9 EF-hand calcium-binding domain-containing protein 9 0,999 9,7 2 2 - 4,2 1 1 0,9991 9,7 2 2 0,9991 9,7 2 5 0,000233

255958156 PRKACB cAMP-dependent protein kinase catalytic subunit beta isoform 4 0,999 15,1 6 7 - 0 0 0 0 0 0 0 0,9999 20,7 9 27 0,000467 261244894 KRT81 keratin, type II cuticular Hb1 0,999 5,4 3 2 - 0 0 0 0 0 0 0 0,9991 5,4 3 2 0,0000337 34610211 Sort1 sortilin isoform 2 precursor 0,999 2,3 2 2 1 4,4 3 3 1 6,8 4 4 1 8 6 8 0,0000956 46430514 4930504O13Rik uncharacterized protein LOC403200 precursor 0,999 12,4 2 2 - 0 0 0 1 18,6 3 6 1 23,4 4 8 0,000418 124487358 1700009N14Rik uncharacterized protein LOC75471 0,9989 8,8 2 2 - 0 0 0 0,9947 19,9 4 3 0,9938 19,9 4 5 0,000149 13386110 Efcab2 EF-hand calcium-binding domain-containing protein 2 0,9989 6,1 2 2 - 0 0 0 1 12,8 3 3 1 12,8 3 5 0,000293 21312872 LRP2BP LRP2-binding protein 0,9989 6,6 2 2 0,9997 5,8 2 2 1 14,7 4 4 1 17,3 5 9 0,000212 23956058 PLP1 myelin proteolipid protein 0,9989 8,7 2 3 - 0 0 0 0 0 0 0 1 11,6 3 4 0,00017 377834422 Prdx1 PREDICTED: peroxiredoxin-1 0,9989 8 2 2 - 0 0 0 0 0 0 0 0 0 4 0 - 6754160 Gm14270 HCLS1-associated protein X-1 0,9989 7,5 2 2 - 3,9 1 1 0 0 0 0 1 11,4 3 3 0,0000972 6755004 AIFM1 apoptosis-inducing factor 1, mitochondrial precursor 0,9989 3,8 2 2 1 4,6 2 3 0 0 0 0 1 9 4 6 0,0000709 244791232 2310028O11Rik mitochondrial inner membrane organizing system protein 1 0,9988 10,5 1 2 - 10,5 1 1 0 0 0 0 0,9989 10,5 1 3 0,000318 PREDICTED: mitochondrial import inner membrane translocase 309268906 Tim14 subunit TIM14-like 0,9988 19,8 2 2 0,999 19,8 2 2 0 0 0 0 0,999 19,8 2 4 0,000195 31543974 YWHAB 14-3-3 protein beta/alpha 0,9988 14,2 3 2 1 19,1 5 7 0,9512 13,4 3 1 1 23,2 6 10 0,000314 9055242 DNAJB1 dnaJ homolog subfamily B member 1 0,9988 5,9 2 2 - 0 0 0 0,9867 4,7 1 1 1 10,6 3 4 0,0000738 113680352 Gm5678 carbonyl reductase [NADPH] 1 0,9987 7,9 2 2 1 15,5 4 4 1 33,2 8 8 1 42,2 10 14 0,000333 CKLF-like MARVEL transmembrane domain-containing protein 21729765 Cmtm2b 2B 0,9987 8,6 2 2 0,9986 8,1 2 2 0,9607 3,8 1 1 0,9999 8,6 3 5 0,000268 6752944 Abcg2 ATP-binding cassette sub-family G member 2 0,9986 3 2 2 - 0 0 0 1 4,9 3 3 1 6,4 4 5 0,0000947 13385186 CCDC96 coiled-coil domain-containing protein 96 0,9985 4,3 2 2 1 5,7 3 3 1 6,7 3 7 1 10,4 5 13 0,00016 158854020 echdc1 ethylmalonyl-CoA decarboxylase isoform a 0,9985 7,4 2 2 1 10 3 3 1 15,7 4 4 1 18,4 5 9 0,000225 12963757 isyna1 inositol-3-phosphate synthase 1 0,9983 3,1 2 2 - 0 0 0 1 6,6 4 4 1 6,6 4 6 0,000131 254553287 Tmem89 transmembrane protein 89 precursor 0,9983 11,8 2 2 0,9993 11,8 2 3 0,9995 11,8 2 2 0,9996 11,8 2 7 0,000691 27754007 NDUFAB1 acyl carrier protein, mitochondrial precursor 0,9983 15,4 2 2 1 15,4 3 3 0,9626 5,8 1 1 1 15,4 3 6 0,000344 21703984 Ubtd1 ubiquitin domain-containing protein 1 0,9981 10,6 2 2 - 0 0 0 0 0 0 0 0 0 2 0 - 9055178 Gm8219 mitochondrial pyruvate carrier 1 0,9981 14,7 2 2 - 0 0 0 0,9976 14,7 2 2 0,9983 14,7 2 4 0,000318 149251558 Gm8808 PREDICTED: 60S ribosomal protein L30-like 0,9979 17,4 2 2 - 0 0 0 0 0 0 0 0,9981 17,4 2 2 0,00011 8394493 Tuba8 tubulin alpha-8 chain 0,9977 35,2 13 5 - 0 0 0 1 55 19 33 1 46,1 18 51 0,000724 serine/threonine-protein phosphatase 4 regulatory subunit 1 isoform 166706860 Ppp4r1 a 0,9976 2,8 2 2 0,9995 2,8 2 2 0 0 0 0 0,9997 2,8 2 4 0,0000379 13385312 PLIN3 perilipin-3 0,9975 4,3 2 2 - 0 0 0 1 8 3 3 1 8 3 5 0,000109 377833208 Pdia6 PREDICTED: protein disulfide-isomerase A6 0,9967 10,2 3 3 0,9993 6,4 2 4 0,9996 20,2 6 6 0,9998 23,8 7 13 0,000302 83999999 #N/A glucosamine-6-phosphate isomerase 2 0,9934 10,1 2 3 1 17 4 4 1 47,1 8 12 1 47,1 8 21 0,000527 PREDICTED: NADH dehydrogenase [ubiquinone] 1 subunit C2- 83004247 LOC675851 like 0,9929 21,7 3 4 - 0 0 0 0,9988 30,8 3 5 0,9999 37,5 4 12 0,000642 21746161 Tubb2a-ps2 tubulin beta-2B chain 0,9909 42,7 14 3 0,9996 45,6 15 6 1 63,8 21 11 1 63,8 21 19 0,000249 110625692 BC061194 uncharacterized protein LOC381350 - 7,5 3 1 1 13,4 5 4 1 33 12 14 1 36,5 13 19 0,000358 110625761 LOC100048880 AFG3-like protein 2 - 0 0 0 1 17,6 14 14 1 17,3 12 12 1 24,6 18 25 0,000209 110626066 MORN3 MORN repeat-containing protein 3 - 0 0 0 1 24,5 5 7 1 36,5 7 7 1 36,5 7 14 0,000383

111038118 hadh hydroxyacyl-coenzyme A dehydrogenase, mitochondrial precursor - 3,5 1 1 1 14,3 3 3 0,9998 6,7 2 2 1 17,8 4 6 0,000174 112293266 HSPA4 heat shock 70 kDa protein 4 - 0 0 0 1 6,5 4 3 1 8,9 5 3 1 11,9 7 7 0,0000602 Réplica 1 Réplica 2 Réplica 3 Datos combinados

Probabilidad Números de Porcentaje Números de Porcentaje Números de Probabilidad Porcentaje Referencia de Porcentaje de péptidos Número de Probabilidad de péptidos Número de Probabilidad de péptidos Número de de de Números de Número de GI Proteína Nombre de la proteína coincidencia cobertura únicos espectros de coincidencia cobertura únicos espectros de coincidencia cobertura únicos espectros coincidencia cobertura péptidos únicos espectros Valor APEX 112983636 KRT10 keratin, type I cytoskeletal 10 - 5 3 6 1 6,6 4 6 1 10,9 6 10 1 10,9 6 22 0,000547 113195678 lamp1 lysosome-associated membrane glycoprotein 1 precursor - 2 1 1 1 7,6 3 5 0 0 0 0 1 7,6 3 6 0,00018 113195690 Man2b1 lysosomal alpha-mannosidase precursor - 2,5 1 1 1 6,5 6 6 1 8,8 6 6 1 15,6 12 14 0,000154 113931154 1600016N20Rik uncharacterized protein C11orf35 homolog - 0 0 0 1 5,3 3 3 1 5,9 3 4 1 7,4 4 6 0,0000814 116089322 lonp1 lon protease homolog, mitochondrial precursor - 0 0 0 1 6 5 6 1 13,8 10 11 1 15,8 12 17 0,000147 118026915 EFCAB5 EF-hand calcium-binding domain-containing protein 5 - 0,5 1 1 1 7,5 6 7 1 9 10 12 1 11,9 12 20 0,000115 124486624 4932425I24Rik protein MAATS1 - 1,3 1 1 1 3,8 3 7 1 7,3 4 8 1 8,2 5 16 0,000172 124486773 Dnahc2 dynein heavy chain 2, axonemal - 0 0 0 1 7,9 30 33 1 0,9 3 3 1 8,2 31 36 0,0000692 124486893 1700009P17Rik UPF0740 protein C1orf192 homolog - 4,8 1 1 1 27 4 8 1 36 5 9 1 36 5 18 0,00150958 124487360 FAM154A uncharacterized protein LOC75811 - 2,1 1 1 1 16,4 7 14 1 33,4 13 45 1 33,4 13 58 0,00110067 124487483 Ogdhl 2-oxoglutarate dehydrogenase-like, mitochondrial - 0 0 0 1 12,7 11 32 1 16,3 13 6 1 18,9 16 12 0,0000999 13195674 Gm13082 ras-related protein Rab-6A isoform 2 - 0 0 0 1 15,4 3 3 0 0 0 0 1 15,4 3 3 0,0000823 13385328 FANK1 fibronectin type 3 and ankyrin repeat domains 1 protein - 3,5 1 1 1 11 4 4 1 24,7 8 9 1 29,7 10 15 0,000285 13385592 LYZL1 lysozyme-like protein 1 precursor - 8,1 1 2 1 23,6 2 3 1 23,6 2 4 1 23,6 2 9 0,000698 13385700 1700028P14Rik uncharacterized protein C9orf135 homolog - 0 0 0 1 43,4 8 10 1 46,5 10 12 1 52,6 12 24 0,000571 13385716 1700019N19Rik uncharacterized protein C10orf82 homolog - 5,5 1 1 1 25,7 6 13 1 40,5 8 15 1 40,5 8 29 0,00128282 13385998 Trap1 heat shock protein 75 kDa, mitochondrial precursor - 1,8 1 1 1 14 8 8 1 11,2 7 7 1 15,2 9 15 0,000154 13386112 H2afb1 histone H2A-Bbd type 1 - 0 0 0 1 27 3 3 0,987 11,7 1 1 1 27 3 4 0,000275 14030781 TSSK6 testis-specific serine/threonine-protein kinase 6 - 4 1 2 1 24,9 7 8 1 22 6 6 1 24,9 7 16 0,000377 147905740 MUC5B mucin 5, subtype B, tracheobronchial precursor - 0 0 0 1 9,4 18 22 1 7 16 22 1 11 23 44 0,00014 153792273 LOC100046510 dynein heavy chain 8, axonemal - 0 0 0 1 18,3 74 144 1 13,1 53 80 1 21,5 87 224 0,00043 157057142 Abca14 ATP-binding cassette, sub-family A (ABC1), member 14 - 0,8 1 1 1 2,3 3 3 0 0 0 0 1 3,1 4 4 0,0000233 157951659 CRISP1 cysteine-rich secretory protein 1 precursor - 0 0 0 1 15,6 3 4 0,9997 11,1 2 2 1 15,6 3 6 0,000201 160298168 Gbas protein NipSnap homolog 2 - 3,2 1 1 1 13,5 3 4 1 35,6 7 8 1 38,4 8 12 0,000441 160298207 GNPAT dihydroxyacetone phosphate acyltransferase - 0 0 0 1 21,7 13 13 1 9,9 6 6 1 24,5 15 19 0,000216 160333185 LOC100047713 mitochondrial intermediate peptidase precursor - 0 0 0 1 10,3 6 7 1 7,3 4 4 1 11,5 7 11 0,000137 160333304 APOA1 apolipoprotein A-I preproprotein - 4,9 1 1 1 27,3 7 11 1 16,3 4 6 1 27,3 7 18 0,000465 161086922 camk4 calcium/calmodulin-dependent protein kinase type IV - 0 0 0 1 9 3 4 1 9,8 3 5 1 14,7 5 9 0,000182 162329596 PPIL6 peptidyl-prolyl cis-trans isomerase-like 6 - 5,1 1 1 1 26,8 5 5 1 27,2 6 8 1 27,2 6 14 0,000477 165377065 Gm5867 UMP-CMP kinase - 4,8 1 1 1 25,1 4 4 0 0 0 0 1 25,1 4 5 0,000145 167234411 NUBPL iron-sulfur protein NUBPL precursor - 5 1 1 1 20,1 4 4 1 17,9 5 5 1 32,9 8 10 0,000275 167716839 COX1 cytochrome c oxidase subunit I [Mus musculus musculus] - 0 0 0 1 6,2 1 5 0 0 0 0 1 6,2 1 5 0,000548 167736371 ube4a ubiquitin conjugation factor E4 A - 0 0 0 1 3,7 3 3 0,9814 1,4 1 1 1 3,7 3 4 0,0000501 169790797 FCGBP Fc fragment of IgG binding protein precursor - 0,3 1 4 1 4 7 5 1 5,2 8 4 1 5,6 9 9 0,0000509 170650601 macrod1 O-acetyl-ADP-ribose deacetylase MACROD1 - 0 0 0 1 13,6 3 3 1 13,6 4 4 1 20,7 5 8 0,000173 171543846 GPX5 epididymal secretory glutathione peroxidase precursor - 5 1 1 1 31,2 6 7 1 24,9 5 6 1 36,2 7 13 0,00042 17505218 GHITM growth hormone-inducible transmembrane protein - 3,2 1 1 1 9,2 3 5 1 9,2 3 3 1 9,2 3 9 0,000317 188219582 LOC231914 glucosamine-6-phosphate isomerase 1 - 7,3 2 1 1 18,3 5 10 1 23,9 5 6 1 30,8 7 15 0,00035 19526463 ERP29 endoplasmic reticulum resident protein 29 precursor - 0 0 0 1 12,2 3 3 0,9865 3,4 1 1 1 15,6 4 4 0,000096

19526759 SPATA19 spermatogenesis-associated protein 19, mitochondrial precursor - 9,7 1 1 1 20,8 2 3 1 37 4 12 1 37 4 16 0,000775 19526858 4930579J09Rik protein phosphatase 1 regulatory subunit 32 - 0 0 0 1 21,3 7 8 1 38,6 13 20 1 38,6 13 28 0,000518 198278509 4930588N13Rik testis-expressed sequence 26 protein - 0 0 0 1 10,8 3 4 0,9586 3 1 1 1 10,8 3 5 0,000157

20070408 Gldc glycine dehydrogenase [decarboxylating], mitochondrial precursor - 0 0 0 1 5,2 4 6 0 0 0 0 1 5,2 4 6 0,0000584 20149730 BSPRY B box and SPRY domain-containing protein - 3,1 1 1 1 7,4 3 5 1 27 13 24 1 27 13 30 0,000586 209862939 Hmox2 heme oxygenase 2 - 0 0 0 1 21,3 4 4 1 36,8 8 11 1 35,9 7 17 0,000395 21312153 glrx5 glutaredoxin-related protein 5, mitochondrial - 9,2 1 1 1 21,7 2 3 0,9838 9,2 1 1 1 21,7 2 5 0,000285 21312734 Sept12 septin-12 isoform 1 - 3,2 1 1 1 12,9 3 3 1 23,7 6 8 1 23,7 6 12 0,000317 21313138 gstk1 glutathione S-transferase kappa 1 - 4,4 1 1 1 35,4 6 6 0,9998 11,5 2 2 1 42,5 7 9 0,000258 21313146 ENKUR enkurin - 2,7 1 1 1 22,4 5 21 1 30,6 7 25 1 33,3 8 46 0,00129444 21313314 EFCAB1 EF-hand calcium-binding domain-containing protein 1 - 3,8 1 1 1 12,7 3 5 0,9992 11,8 2 4 1 20,8 4 11 0,000348 21313338 4933411K16Rik uncharacterized protein C10orf62 homolog - 0 0 0 1 21,1 4 8 1 21,4 5 15 1 21,4 5 23 0,000901

21313484 Gm16437 iron-sulfur cluster assembly enzyme ISCU, mitochondrial precursor - 0 0 0 1 23,8 3 3 0,9513 5,4 1 1 1 23,8 3 4 0,000127 21389313 4921507P07Rik uncharacterized protein C7orf31 homolog - 0 0 0 1 15 7 9 1 15 7 7 1 16,8 8 16 0,000291

21450277 ATP1A1 sodium/potassium-transporting ATPase subunit alpha-1 precursor - 0 0 0 1 14,8 12 5 0,9999 9,9 9 3 1 14,8 12 8 0,0000688 21450325 BLVRB flavin reductase (NADPH) - 0 0 0 1 25,2 3 5 0 0 0 0 1 25,2 3 5 0,000206 21699068 PITRM1 presequence protease, mitochondrial precursor - 0 0 0 1 26,4 19 26 1 35 24 27 1 39,8 27 53 0,000437 223468646 SH3GL3 endophilin-A3 isoform 1 - 0 0 0 1 18,4 5 6 0,9991 7,5 2 3 1 18,4 5 9 0,000192 224994182 PROM2 prominin-2 isoform 1 precursor - 0 0 0 1 7,4 5 5 0 0 0 0 1 7,4 5 5 0,0000569 225579120 Pdzk1 Na(+)/H(+) exchange regulatory cofactor NHE-RF3 - 1,5 1 1 1 23,5 9 9 0,9999 6,4 3 3 1 25,4 10 12 0,000138 226958332 SIGLEC1 sialoadhesin precursor - 0 0 0 1 6 8 12 1 9,2 13 14 1 10,6 15 26 0,000196 227430395 Lrrc57 leucine-rich repeat-containing protein 57 isoform b - 0 0 0 1 14,2 3 5 1 26,4 6 10 1 26,4 6 15 0,000429 227500281 etfA electron transfer flavoprotein subunit alpha, mitochondrial - 5,1 1 1 1 26,7 6 7 1 17,4 4 5 1 35,4 8 13 0,000253 227908837 Pecr peroxisomal trans-2-enoyl-CoA reductase - 0 0 0 1 11,9 3 3 0,9661 3 1 1 1 11,9 3 4 0,000121 229577281 UCHL5 ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 1 - 3,4 1 1 1 18 5 5 1 30,8 7 7 1 35,4 9 13 0,000342 23346543 Gzmn granzyme N precursor - 5,2 1 1 1 17,7 4 5 0,9871 5,2 1 2 1 17,7 4 8 0,000304 Réplica 1 Réplica 2 Réplica 3 Datos combinados

253735751 Pdilt protein disulfide-isomerase-like protein of the testis precursor - 2 1 1 1 5,1 3 3 0,9997 4,9 2 2 1 8 4 7 0,0000905

254039596 iscA2 iron-sulfur cluster assembly 2 homolog, mitochondrial precursor - 0 0 0 1 28,6 4 4 1 36,4 5 5 1 41,6 6 9 0,00029

254540162 pccA propionyl-CoA carboxylase alpha chain, mitochondrial precursor - 0 0 0 1 4,3 3 3 0 0 0 0 1 4,3 3 3 0,0000263 254675147 1700011E24Rik uncharacterized protein C2orf61 homolog - 4,5 1 1 1 13 3 5 0,9997 10,1 2 2 1 13 3 8 0,000217 254675238 KIF9 kinesin-like protein KIF9 isoform 1 - 0 0 0 1 10,1 8 9 0 0 0 0 1 18,8 12 29 0,000294 254692843 Dnahc10 dynein heavy chain 10, axonemal - 0 0 0 1 6,4 24 24 1 1,6 7 7 1 7 27 32 0,0000544 254692940 TTC18 tetratricopeptide repeat domain 18 isoform 3 - 1,2 1 1 1 7,3 7 14 1 10,8 9 11 1 13 11 26 0,000243 254750640 1700061J05Rik testis-expressed sequence 33 protein isoform 1 - 0 0 0 1 14,1 3 5 0,9995 9,1 2 3 1 14,1 3 8 0,000206 255003729 EFHB EF-hand domain-containing family member B - 0 0 0 1 21,3 13 16 1 19,3 13 14 1 27,3 17 30 0,000284 255003777 GLOD4 glyoxalase domain-containing protein 4 - 3,4 1 1 1 26,8 6 6 1 15,4 4 5 1 30,2 7 12 0,000237 255069795 vars valine--tRNA ligase - 0,6 1 1 1 5,8 5 5 1 2,8 3 3 1 7,9 8 9 0,0000586 255683447 LOC100046727 IQ and ubiquitin-like domain-containing protein - 0 0 0 1 9,1 6 7 1 13,5 9 9 1 15,9 11 15 0,000157 255759902 MAOA amine oxidase [flavin-containing] A - 0 0 0 1 7 3 6 1 14,6 6 14 1 14,6 6 21 0,000311 256773234 Dnahc1 dynein, axonemal, heavy chain 1 - 0 0 0 1 11,6 39 42 1 7,9 23 27 1 14,9 48 72 0,000149 257467625 FCGBP Fc fragment of IgG binding protein-like precursor - 0 0 0 1 18,4 33 99 1 15,9 28 61 1 22,2 39 169 0,000772 257467659 Dnahc6 axonemal dynein heavy chain - 0 0 0 1 9,9 37 46 1 5,6 20 26 1 12,1 44 74 0,000144 268370090 WDR64 WD repeat-containing protein 64 - 1,2 1 1 1 10,1 10 13 1 8,6 8 10 1 15,7 15 24 0,000189 27229039 TXNDC8 thioredoxin domain-containing protein 8 - 5,9 1 1 1 19,6 3 3 0,9993 13,7 2 2 1 19,6 3 6 0,000251 27369597 A330021E22Rik uncharacterized protein C7orf63 homolog - 1,2 1 1 1 6,9 5 5 1 4,6 4 4 1 8,9 7 10 0,0000934 27501448 eIF6 eukaryotic translation initiation factor 6 - 4,1 1 1 1 21,6 4 5 1 36,7 5 5 1 41,2 6 11 0,000542 28077051 NLN neurolysin, mitochondrial precursor - 1,4 1 1 1 14,8 9 9 1 11,9 6 8 1 22,3 12 18 0,000217

281427153 TMED3 transmembrane emp24 domain-containing protein 3 precursor - 0 0 0 1 8,1 2 3 0 0 0 0 1 8,1 2 3 0,000134 281604088 Gm7655 isopentenyl-diphosphate Delta-isomerase 1 - 4,2 1 1 1 11 3 3 1 20,8 5 5 1 24,4 6 9 0,000231 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 282398108 RPN1 subunit 1 precursor - 2 1 1 1 18,3 9 11 0,983 2,8 1 1 1 18,3 9 13 0,000152 282721066 4931409K22Rik IQ and AAA domain-containing protein 1-like - 1,5 1 2 1 4,1 3 6 1 4,2 3 7 1 6,9 5 15 0,000118 282721112 CASC1 cancer susceptibility candidate protein 1 - 0 0 0 1 12,5 8 10 1 11 7 12 1 12,5 8 22 0,000245 283837762 Dnahc17 dynein heavy chain 17, axonemal - 0 0 0 1 19,8 74 113 1 16,7 57 80 1 24,7 91 197 0,000377 28916687 rab5b ras-related protein Rab-5B isoform 1 - 0 0 0 1 27,9 5 4 1 28,8 5 3 1 34,4 6 7 0,000256 28916703 Prdx6-rs1 peroxiredoxin 6, related sequence 1 - 0 0 0 1 45,1 7 5 0,9842 18,3 3 1 1 45,1 7 6 0,000146 294460010 - uncharacterized protein LOC69439 - 1 1 1 1 5,3 4 5 1 6,6 5 5 1 10,6 8 11 0,000112 295054193 Odf2 outer dense fiber protein 2 isoform e - 0 0 0 1 45,6 30 176 0,9848 43,1 27 1 1 48,6 32 462 0,00316617 29568082 GMPR2 GMP reductase 2 - 0 0 0 1 8,9 3 3 0,9679 2,6 1 1 1 8,9 3 4 0,0000916 295789144 - UPF0704 protein C6orf165 homolog - 0 0 0 1 5 3 3 1 5,4 3 3 1 8,3 5 6 0,000103 29789289 Echs1 enoyl-CoA hydratase, mitochondrial precursor - 0 0 0 1 14,1 4 4 0,9992 10,3 2 2 1 21,4 5 6 0,000155 30425280 4930412F15Rik protein FAM221B - 3,1 1 1 1 9,7 3 3 1 8,4 3 3 1 18,1 6 6 0,000117 30578432 SIRT5 NAD-dependent protein deacylase sirtuin-5, mitochondrial - 0 0 0 1 21,3 5 5 1 12,3 3 3 1 26,1 7 9 0,000227 307344688 Wdr96 WD repeat domain 96 - 0,9 1 1 1 11,6 16 25 1 9 11 13 1 14,1 19 37 0,000205 307691211 - regulator of G-protein signaling 22 - 0 0 0 1 6 8 11 0,987 1,1 1 1 1 7,1 9 12 0,0000841 30794150 man2c1 alpha-mannosidase 2C1 - 1,4 1 2 1 14,2 9 10 1 17,1 13 13 1 23,1 16 26 0,00027 30841008 RAB18 ras-related protein Rab-18 isoform 2 precursor - 5,3 1 2 1 30,1 5 5 1 36,4 6 6 1 41,7 7 13 0,000345 309262380 - PREDICTED: uncharacterized protein C17orf47 homolog - 2,1 1 4 1 14,7 9 26 1 12,3 7 21 1 16,2 10 50 0,000809 309267418 Dnah7 PREDICTED: dynein heavy chain 7, axonemal - 0 0 0 1 14,6 53 20 1 10,6 35 12 1 19,7 69 34 0,0000668 PREDICTED: LOW QUALITY PROTEIN: dynein heavy chain 3, 309268955 dnhc3 axonemal - 0 0 0 1 13 43 49 1 5,3 17 18 1 14,8 48 67 0,000134 31542143 Gm12844 transforming protein RhoA precursor - 0 0 0 1 16,1 3 3 0 0 0 0 0,9999 29,5 5 13 0,000495 31543330 Nnt NAD(P) transhydrogenase, mitochondrial precursor - 0 0 0 1 16,1 14 15 1 11 9 9 1 17,7 15 24 0,000272 31559918 NRD1 nardilysin precursor - 0 0 0 1 7 7 7 0,9997 2,5 2 2 1 7 7 9 0,0000749 31560677 LTF lactotransferrin precursor - 0 0 0 1 9,8 6 6 0,966 1,4 1 1 1 9,8 6 7 0,0000646 319655703 Ccdc136 coiled-coil domain-containing protein 136 isoform 1 - 0 0 0 1 9,6 6 10 0 0 0 0 1 13,7 9 20 0,000179

31981013 msrA mitochondrial peptide methionine sulfoxide reductase isoform 1 - 0 0 0 1 27,5 4 6 0 0 0 0 1 27,5 4 6 0,00019 31982273 hsd17b4 peroxisomal multifunctional enzyme type 2 - 0 0 0 1 8,2 5 5 0,9998 3,5 2 2 1 8,2 5 7 0,0000786 Réplica 1 Réplica 2 Réplica 3 Datos combinados

Probabilidad Números de Porcentaje Números de Porcentaje Números de Probabilidad Porcentaje Referencia de Porcentaje de péptidos Número de Probabilidad de péptidos Número de Probabilidad de péptidos Número de de de Números de Número de GI Proteína Nombre de la proteína coincidencia cobertura únicos espectros de coincidencia cobertura únicos espectros de coincidencia cobertura únicos espectros coincidencia cobertura péptidos únicos espectros Valor APEX 31982710 FSCN3 fascin-3 - 1,6 1 1 1 7 3 3 1 15,3 6 10 1 15,3 6 14 0,000308 32469507 Cd59b CD59B glycoprotein precursor - 7 1 1 1 23,3 3 3 0,973 7 1 1 1 23,3 3 5 0,000222 33859482 Gm13050 elongation factor 2 - 1,6 1 1 1 7 5 6 1 4,2 3 3 1 11,2 8 10 0,0000808 33859811 HADHA trifunctional enzyme subunit alpha, mitochondrial precursor - 1,3 1 1 1 25,4 16 27 1 43 25 46 1 44,4 26 74 0,00067 34328334 Acox3 peroxisomal acyl-coenzyme A oxidase 3 - 2,1 1 1 1 16,3 8 16 1 24,1 12 14 1 30,6 15 34 0,000379 354983491 Dnahc7a dynein, axonemal, heavy chain 7A - 0 0 0 1 13,9 49 8 0,9801 9,5 31 2 1 18,3 63 23 0,0000457 359339012 Smrp1 spermatid-specific manchette-related protein 1 isoform 3 - 0 0 0 1 52 14 27 1 59,5 14 55 1 61,8 15 60 0,00114571 363543275 Adgb androglobin - 0 0 0 1 4 5 5 1 6,1 6 6 1 8,2 9 10 0,0000544 pyruvate dehydrogenase phosphatase regulatory subunit, 38142488 4930402E16Rik mitochondrial precursor - 0 0 0 1 5,9 5 5 1 6,6 5 5 1 10,1 8 10 0,000111 38348576 Odf3l1 outer dense fiber protein 3-like protein 1 - 0 0 0 1 20,7 4 5 1 19,6 4 7 1 27,6 6 12 0,000396 38604071 xpo1 exportin-1 - 1,4 1 1 1 5,8 4 4 1 6,3 5 5 1 8,9 7 11 0,000107 407027856 Capzb F-actin-capping protein subunit beta isoform c - 0 0 0 1 49,5 12 28 1 46,5 12 28 1 49,8 13 61 0,00122357 407261456 Lipe PREDICTED: hormone-sensitive lipase-like - 0 0 0 1 20,7 3 4 1 18,3 3 3 1 30,2 5 8 0,000299 407262105 Dnhc12 PREDICTED: dynein heavy chain 12, axonemal - 0 0 0 1 10,3 35 49 1 5,7 17 20 1 13,3 44 69 0,000137 407263255 - PREDICTED: WD repeat-containing protein 66 - 0 0 0 1 12,9 11 13 1 12,4 10 11 1 19,2 16 25 0,000163 40789280 FAF1 FAS-associated factor 1 - 0 0 0 1 5,1 3 3 0 0 0 0 1 5,1 3 3 0,0000454 40807498 Hrsp12 ribonuclease UK114 - 10,4 1 1 1 37,8 4 4 1 29,6 3 3 1 45,2 5 8 0,000349 45476581 SCP2 non-specific lipid-transfer protein - 0 0 0 1 7,3 4 4 0,9999 4,9 3 3 1 10,4 6 7 0,000112 46361984 HYDIN hydrocephalus-inducing protein - 0 0 0 1 3,1 14 16 1 3,1 12 16 1 5,8 24 35 0,0000673 46559389 ACE angiotensin-converting enzyme isoform 1 precursor - 0 0 0 1 18,6 19 49 1 21,6 21 39 1 24,3 25 119 0,000941 47564127 BC048507 dynein light chain LC8-type 1-like - 29,2 3 1 1 37,1 3 3 1 50,6 5 5 1 59,6 6 10 0,000635 N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase isoform 1 54292135 aga precursor - 0 0 0 1 29,5 7 7 1 24 5 6 1 29,5 7 17 0,000465 58037191 RAB28 ras-related protein Rab-28 - 8,1 1 1 1 34,4 6 7 1 28,5 5 5 1 38,9 7 13 0,000365 58037271 1700023E05Rik testicular haploid expressed gene protein-like - 0 0 0 1 9,4 3 4 1 17 6 13 1 19,2 7 17 0,000258 58037363 SPATC1 speriolin - 0 0 0 1 8,5 3 6 1 18,3 6 28 1 18,3 6 34 0,000883 58037409 Phpt1 14 kDa phosphohistidine phosphatase - 9,7 1 2 1 28,2 3 4 1 28,2 3 5 1 28,2 3 11 0,000535 bifunctional methylenetetrahydrofolate 6678952 mthfd2 dehydrogenase/cyclohydrolase, mitochondrial precursor - 0 0 0 1 9,4 3 3 0 0 0 0 0,9995 6,6 2 2 0,0000529 6679465 prkcsh glucosidase 2 subunit beta precursor - 0 0 0 1 6,3 3 3 0,976 2,3 1 2 1 6,3 3 5 0,000131 6679891 GANAB neutral alpha-glucosidase AB - 0 0 0 1 4,3 4 6 0,9842 1,6 1 1 1 4,3 4 6 0,0000606 6680019 GCLM glutamate--cysteine ligase regulatory subunit - 0 0 0 1 12,8 3 3 0,9998 10,2 2 2 1 12,8 3 5 0,000233 6754976 Gm7204 peroxiredoxin-1 - 0 0 0 1 29,1 5 7 1 32,2 7 12 1 43,2 8 21 0,000616 6755120 Sept4 septin-4 - 0 0 0 1 6,9 3 8 1 20,9 8 27 1 20,9 8 36 0,000581

6755967 Gm7591 voltage-dependent anion-selective channel protein 3 isoform 2 - 0 0 0 1 44,2 9 20 1 50,2 10 23 1 53 11 51 0,00112712 6756039 LOC674211 14-3-3 protein theta - 0 0 0 1 19,2 5 4 0 0 0 0 1 23,3 6 5 0,000143 70887605 SPAG16 sperm-associated antigen 16 protein isoform 1 - 0 0 0 1 6,7 4 6 1 9,7 6 9 1 11 7 16 0,000226 7305035 Epb4.2 erythrocyte membrane protein band 4.2 - 0 0 0 1 7,7 4 4 0 0 0 0 1 7,7 4 4 0,0000559 mitochondrial translocator assembly and maintenance protein 41 76559944 1500001M20Rik homolog precursor - 3,9 1 1 1 9,2 3 4 0,9996 6,5 2 3 1 9,8 3 7 0,000234 7656912 ATP8A2 probable phospholipid-transporting ATPase IB - 1 1 1 1 3,1 3 3 0,9999 3,3 3 3 1 5,3 5 7 0,0000568 76880494 Ube3a ubiquitin-protein ligase E3A isoform 2 - 0 0 0 1 6,6 5 5 0 0 0 0 1 6,6 5 5 0,0000422 78711830 Adrbk2 beta-adrenergic receptor kinase 2 isoform 1 - 0 0 0 1 9 5 5 0 0 0 0 1 9 5 5 0,0000553 PREDICTED: membrane-spanning 4-domains subfamily A 82998851 Ms4a14 member 14 - 0 0 0 1 6,8 5 6 0 0 0 0 1 8,6 7 12 0,000198 8393988 PMM2 phosphomannomutase 2 - 0 0 0 1 12,8 3 3 1 10,7 3 3 1 20,2 5 6 0,000129 84871986 GPX1 glutathione peroxidase 1 - 0 0 0 1 19,4 3 3 0,9991 13,4 2 2 1 27,9 4 5 0,000173 85662421 ACTL9 actin-like protein 9 - 4,1 1 1 1 8,2 3 3 1 10,6 3 3 1 12,3 4 7 0,000203 85701754 Ccdc114 coiled-coil domain-containing protein 114 - 2,4 1 1 1 8,1 4 6 1 7,6 4 5 1 12,3 6 13 0,000163 87162476 ccdc108 coiled-coil domain-containing protein 108 - 0 0 0 1 2,5 4 6 1 2,1 4 4 1 3,4 6 10 0,000064 93102417 Gars glycine--tRNA ligase - 0 0 0 1 4,4 3 3 0,9705 1,4 1 1 1 5,8 4 4 0,0000393 149260287 LOC100045999 PREDICTED: GTP-binding nuclear protein Ran-like - 0 0 0 0,9999 13,4 3 3 0,9998 25 5 4 0,9998 25 5 7 0,000242 6755206 psmb7 proteasome subunit beta type-7 precursor - 0 0 0 0,9999 11,2 3 3 0,9735 3,6 1 1 0,9999 11,2 3 5 0,000148 124301182 9230107M04Rik binder of sperm protein homolog 2 precursor - 5,3 1 1 0,9998 17,6 2 2 0,9659 5,3 1 1 1 22,9 3 4 0,0003 133778926 Enah protein enabled homolog isoform 1 - 2 1 1 0,9998 5,9 2 2 0,9991 4,3 2 2 1 8,1 3 5 0,0000741 14149635 FABP4 fatty acid-binding protein, adipocyte - 9,8 1 1 0,9998 18,9 2 2 0,9998 18,9 2 2 0,9998 18,9 2 5 0,00019 15617197 Gm13453 V-type proton ATPase subunit G 1 - 0 0 0 0,9998 22 2 2 0,9839 9,3 1 1 0,9998 22 2 3 0,000204 254587949 Dnajc5b dnaJ homolog subfamily C member 5B - 10,1 1 2 0,9998 17,1 2 3 0,9802 10,1 1 2 0,9998 17,1 2 7 0,000301 55925585 Spag8 sperm-associated antigen 8 - 0 0 0 0,9998 6,8 2 2 0,9832 3,9 1 1 0,9998 6,8 2 3 0,0000842 96975138 hprT hypoxanthine-guanine phosphoribosyltransferase - 6 1 1 0,9998 11,5 2 2 1 16,1 3 3 1 22 4 6 0,000176 113462019 LOC100047516 casein kinase I isoform gamma-3 - 0 0 0 0,9997 5,1 2 2 0 0 0 0 0 0 4 0 - 146260278 GPX6 glutathione peroxidase 6 precursor - 5,4 1 1 0,9997 5,4 1 2 0,9998 5,4 1 2 0,9998 5,4 1 5 0,000243 149247503 LOC100046536 PREDICTED: glutathione S-transferase P 2-like - 0 0 0 0,9997 15,2 2 2 0 0 0 0 0,9998 15,2 2 2 0,000107 154240699 4930505A04Rik uncharacterized protein C2orf73 homolog - 0 0 0 0,9997 9,4 2 3 0,9571 4,3 1 1 0,9997 9,4 2 4 0,000151 157952206 bag1 BAG family molecular chaperone regulator 1 isoform 1L - 0 0 0 0,9997 12,3 2 2 0 0 0 0 0,9997 12,3 2 2 0,0000452

21312189 iscA1 iron-sulfur cluster assembly 1 homolog, mitochondrial precursor - 0 0 0 0,9997 19,4 2 2 0,9995 19,4 2 2 0,9997 19,4 2 4 0,000169 Réplica 1 Réplica 2 Réplica 3 Datos combinados

33563266 Ndufa4 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 - 14,6 1 1 0,9997 26,8 2 2 0,9989 26,8 2 2 0,9998 26,8 2 5 0,000373 34328471 RALA ras-related protein Ral-A precursor - 0 0 0 0,9997 11,2 2 2 0,999 28,2 4 5 1 32,5 5 7 0,000249 40254263 Ubtd2 ubiquitin domain-containing protein 2 - 0 0 0 0,9997 9,4 2 2 0,9871 8,5 1 1 1 28,2 5 5 0,000202

47058964 SSBP1 single-stranded DNA-binding protein, mitochondrial isoform 2 - 0 0 0 0,9997 15,5 2 2 0 0 0 0 0 0 4 0 - 48717492 PRSS48 serine protease 48 precursor - 0 0 0 0,9997 6,7 2 2 0,9833 3,5 1 1 0,9998 6,7 2 3 0,0000854 84370322 Defb42 beta-defensin 42 precursor - 0 0 0 0,9997 14,7 2 2 0,9806 13,3 1 1 0,9997 14,7 2 3 0,000213 85701967 CCDC27 coiled-coil domain-containing protein 27 - 0 0 0 0,9997 3,6 2 3 0 0 0 0 1 5,2 3 4 0,0000416 9790191 Gm9840 E3 ubiquitin-protein ligase RBX1 - 16,7 1 1 0,9997 24,1 2 2 0,9996 24,1 2 2 0,9997 24,1 2 5 0,000503 10946870 Akr1a4 alcohol dehydrogenase [NADP(+)] - 0 0 0 0,9996 7,1 2 2 0 0 0 0 0,9997 7,1 2 2 0,0000431 148540106 MUT methylmalonyl-CoA mutase, mitochondrial precursor - 0 0 0 0,9996 3,2 2 2 1 6,4 4 4 1 6,4 4 6 0,0000644 15011841 gpX3 glutathione peroxidase 3 precursor - 0 0 0 0,9996 18,1 3 2 0,9996 18,1 3 2 0,9997 18,1 3 4 0,000157 157909797 tomm40 mitochondrial import receptor subunit TOM40 homolog - 4,4 1 1 0,9996 8,6 2 2 1 26,6 6 9 1 26,6 6 12 0,000376 160420337 ENO4 enolase-like protein ENO4 - 1,8 1 1 0,9996 3,9 2 2 0 0 0 0 0,9997 3,9 2 3 0,0000538 168693651 Dnalc4 dynein light chain 4, axonemal - 9,5 1 1 0,9996 16,2 2 2 1 29,5 3 4 1 38,1 4 7 0,000453 19882207 LOC674674 farnesyl pyrophosphate synthase isoform 2 - 2,3 1 1 0,9996 6,2 2 2 1 14,7 5 5 1 14,7 5 8 0,000213 21314844 CATSPER1 cation channel sperm-associated protein 1 - 1,9 1 1 0,9996 1,9 1 2 0,9998 1,9 1 2 0,9998 1,9 1 5 0,000143 21704066 LOC100046373 ras-related protein Rap-1A precursor - 0 0 0 0,9996 11,4 2 2 0,9987 10,3 2 2 1 15,8 3 4 0,000138

23956162 AGPAT2 1-acyl-sn-glycerol-3-phosphate acyltransferase beta precursor - 0 0 0 0,9996 6,8 2 3 0,9998 6,8 2 2 1 6,8 2 6 0,000183 242117962 1700029G01Rik SH3 domain-containing protein 21 isoform 1 - 0 0 0 0,9996 3,5 2 2 0 0 0 0 0,9996 3,5 2 2 0,000023 254587916 1700094D03Rik uncharacterized protein C1orf189 homolog - 0 0 0 0,9996 22,6 2 2 0,9858 13,2 1 1 0,9997 22,6 2 3 0,000176 257196215 Ccdc116 coiled-coil domain-containing protein 116 - 0 0 0 0,9996 3,7 1 2 1 5,2 2 8 1 5,2 2 10 0,00022 30525051 Rab2b ras-related protein Rab-2B - 0 0 0 0,9996 45,8 10 2 0,9998 46,3 11 2 1 52,8 12 5 0,000142 31982511 LOC635419 alcohol dehydrogenase class-3 - 0 0 0 0,9996 6,4 2 2 0 0 0 0 0,9996 6,4 2 2 0,0000469 34328278 Lman2 vesicular integral-membrane protein VIP36 precursor - 0 0 0 0,9996 6,4 2 3 0 0 0 0 1 13,7 3 4 0,0000816 357197128 Ranbp3 ran-binding protein 3 isoform 1 - 0 0 0 0,9996 3,9 2 2 0 0 0 0 0,9997 3,9 2 2 0,0000395 46358378 Abca15 ATP-binding cassette transporter sub-family A member 15 - 0 0 0 0,9996 1,8 2 2 0 0 0 0 0,9996 1,8 2 2 0,0000139 6677917 SEPW1 selenoprotein W - 11,4 1 1 0,9996 12,5 2 3 0,9829 11,4 1 2 0,9996 12,5 2 6 0,000435 6678437 Gm1974 translationally-controlled tumor protein - 8,1 1 1 0,9996 8,1 1 2 0,9992 15,7 2 2 0,9997 8,1 1 4 0,000252 6678760 Lypla1 acyl-protein thioesterase 1 - 5,7 1 1 0,9996 10,4 2 2 0,9997 10,4 2 2 0,9997 10,4 2 5 0,000212 6680502 itm2b integral membrane protein 2B - 0 0 0 0,9996 7,9 2 2 1 12 3 3 1 16,2 4 5 0,000122 84370330 GLRX2 glutaredoxin-2, mitochondrial isoform b - 0 0 0 0,9996 16,3 2 2 0,9995 23,6 2 2 1 31,7 3 4 0,000268 91206392 MPI mannose-6-phosphate isomerase - 3,5 1 1 0,9996 7,3 2 2 0,9998 7,1 2 2 1 10,9 3 5 0,000114 109948267 4930578I06Rik uncharacterized protein C8orf74 homolog - 3,7 1 1 0,9995 11,1 2 2 1 26,2 6 6 1 26,2 6 9 0,000266 125991758 SPEF2 sperm flagellar protein 2 - 0 0 0 0,9995 2,7 2 3 0,9995 2,6 2 3 1 3,8 3 6 0,0000582 149240955 - PREDICTED: casein kinase II subunit alpha-like - 0 0 0 0,9995 8,2 2 2 0 0 0 0 0 0 5 0 - 160358385 ART3 ecto-ADP-ribosyltransferase 3 precursor - 2,8 1 1 0,9995 3 2 2 0,9995 9,4 2 2 1 9,7 3 5 0,000138 160707911 ank1 ankyrin-1 isoform 2 - 0,7 1 1 0,9995 1,2 2 3 1 1,8 3 4 1 2,5 4 8 0,0000363 210031252 chdh choline dehydrogenase, mitochondrial - 2,2 1 2 0,9995 3,7 2 2 1 29,9 13 20 1 24,8 12 22 0,000316 21311897 ASB9 ankyrin repeat and SOCS box protein 9 - 0 0 0 0,9995 7,9 2 2 1 12,4 4 5 1 12,4 4 7 0,000319 21312758 PPP6C serine/threonine-protein phosphatase 6 catalytic subunit - 4,3 1 1 0,9995 10,5 2 2 0,9994 7,9 2 2 1 18,4 4 5 0,000122 29244400 Spink10 serine protease inhibitor Kazal-type 10 precursor - 4,9 1 1 0,9995 11,7 2 2 0 0 0 0 0,9995 11,7 2 3 0,000166 30350218 CATSPER3 cation channel sperm-associated protein 3 isoform 2 - 4,4 1 1 0,9995 6,7 2 2 0 0 0 0 0,9996 6,7 2 3 0,000111 308082030 Styxl1 serine/threonine/tyrosine-interacting-like protein 1 - 0 0 0 0,9995 8,4 2 2 1 20,6 5 8 1 20,9 5 9 0,000222 309267298 Tat1 PREDICTED: testis anion transporter 1 - 1,4 1 1 0,9995 1,4 1 2 0,9829 1,4 1 1 0,9997 1,4 1 4 0,0000585 312433980 Herc4 probable E3 ubiquitin-protein ligase HERC4 isoform 1 - 0 0 0 0,9995 2,6 2 2 0 0 0 0 0,9995 2,6 2 2 0,0000182 377834275 - PREDICTED: uncharacterized protein LOC100862214 - 0 0 0 0,9995 8,7 2 5 1 19,7 5 8 1 19,7 5 13 0,000382 6755911 Txn1 thioredoxin - 0 0 0 0,9995 21 2 2 0,9629 8,6 1 1 0,9996 21 2 3 0,00016 87080819 Slco6b1 solute carrier organic anion transporter family, member 6b1 - 0 0 0 0,9995 4,4 2 2 0,9815 2,7 1 1 0,9997 4,4 2 3 0,0000571 113374150 1700016C15Rik uncharacterized protein C1orf100 homolog - 0 0 0 0,9994 17,2 2 2 1 43,4 5 8 1 43,4 5 11 0,000725 115430101 C4bp C4b-binding protein precursor - 0 0 0 0,9994 4,9 2 2 0,9995 4,9 2 3 0,9995 4,9 2 5 0,0000984 13385392 napA alpha-soluble NSF attachment protein - 0 0 0 0,9994 5,8 2 2 0,9814 3,4 1 2 1 10,2 3 5 0,000109 13385842 4921517D21Rik uncharacterized protein LOC67722 - 0 0 0 0,9994 2,2 2 3 0,9865 1 1 5 0,9998 2,2 2 8 0,0000754 157041252 PLIN4 perilipin-4 - 0 0 0 0,9994 6,3 2 2 0 0 0 0 0,9995 6,3 2 2 0,00000899 157951602 Wfdc16 WAP four-disulfide core domain 16 - 11,7 1 1 0,9994 11,7 1 2 0 0 0 0 1 11,7 1 3 0,000445 170172516 stx2 syntaxin-2 - 3,1 1 2 0,9994 8 2 2 0 0 0 0 0,9996 8 2 4 0,000114 21644577 abhd11 alpha/beta hydrolase domain-containing protein 11 isoform 1 - 5,7 1 2 0,9994 8,8 2 2 1 20,3 3 3 1 32,2 5 8 0,000302 calcium/calmodulin-dependent 3',5'-cyclic nucleotide 229577068 Pde1c phosphodiesterase 1C isoform c - 0 0 0 0,9994 4,6 2 2 0 0 0 0 0,9995 4,6 2 2 0,000026 254588112 1700021F07Rik uncharacterized protein C20orf85 homolog - 0 0 0 0,9994 15,4 2 2 0,9741 8,8 1 1 0,9994 15,4 2 3 0,000162 254675129 ANKRD45 ankyrin repeat domain-containing protein 45 - 3,9 1 1 0,9994 6,7 2 2 0,9998 9,6 2 3 1 12,4 3 6 0,000175 Réplica 1 Réplica 2 Réplica 3 Datos combinados

407261032 Cox7c PREDICTED: cytochrome c oxidase subunit 7C, mitochondrial-like - 14,3 1 1 0,9994 28,6 2 2 0,9821 14,3 1 1 0,9996 28,6 2 4 0,000456 52421794 pcbd2 pterin-4-alpha-carbinolamine dehydratase 2 - 0 0 0 0,9994 15,4 2 2 0,9856 7,4 1 1 0,9995 15,4 2 3 0,000111 58037359 1700011F03Rik testis-expressed sequence 37 protein - 6,1 1 1 0,9994 12,8 2 2 1 52,8 7 19 1 52,8 7 22 0,00104277 6679587 Rab1 ras-related protein Rab-1A - 5,9 1 1 0,9994 12,7 2 2 0,9745 5,9 1 1 1 18,5 3 4 0,000112 110625742 1700007K13Rik UPF0691 protein C9orf116 homolog - 0 0 0 0,9993 10,8 1 2 1 45,5 6 12 1 45,5 6 13 0,000824 117676359 Prss52 serine protease 52 precursor - 0 0 0 0,9993 7,8 2 3 0,9583 4 1 1 0,9995 7,8 2 4 0,000126 13385090 COX6B1 cytochrome c oxidase subunit 6B1 - 16,3 1 1 0,9993 23,3 2 2 1 37,2 3 3 1 47,7 4 6 0,000274 PREDICTED: NADH dehydrogenase [ubiquinone] 1 alpha 149267300 Ndufa11 subcomplex subunit 11-like - 0 0 0 0,9993 23,1 2 2 0 0 0 0 0 0 2 0 - 162287063 Cnnm4 metal transporter CNNM4 precursor - 0 0 0 0,9993 2,9 2 2 0,9994 3,2 2 3 1 4,3 3 5 0,0000579 227497595 Adcy10 adenylate cyclase type 10 - 0 0 0 0,9993 1,7 2 2 1 2,5 3 3 1 4,3 5 5 0,0000282 256000799 Gm5617 uncharacterized protein C11orf71 homolog - 9,4 1 1 0,9993 17,9 2 2 0 0 0 0 0,9995 17,9 2 3 0,000175 26080429 Aldh16a1 aldehyde dehydrogenase family 16 member A1 - 1,7 1 1 0,9993 3,7 2 2 0 0 0 0 1 5,5 3 3 0,0000484 29789199 LOC100044315 importin-5 - 0 0 0 0,9993 2,2 2 2 1 3,9 3 3 1 6,1 5 5 0,0000479 52138534 BC048562 uncharacterized protein LOC434439 - 0 0 0 0,9993 10,3 1 3 1 41,7 5 11 1 41,7 5 14 0,000832 6753624 Defb2 beta-defensin 2 precursor - 0 0 0 0,9993 39,4 2 2 0,9693 11,3 1 1 0,9995 39,4 2 3 0,000612 140970505 4932411G14Rik uncharacterized protein LOC238663 - 0 0 0 0,9992 1,8 2 2 0,9546 1 1 1 1 1,8 2 3 0,0000249 140970573 RABEP2 rab GTPase-binding effector protein 2 - 0 0 0 0,9992 3,8 2 2 0,9996 4 2 2 1 5,6 3 4 0,000049

161484668 PPP1CB serine/threonine-protein phosphatase PP1-beta catalytic subunit - 23,5 6 1 0,9992 23,2 6 2 0,9821 17,1 5 1 1 33,9 9 4 0,000104 27229278 AGPAT3 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma - 2,4 1 1 0,9992 4,5 2 2 1 15,7 5 5 1 17,8 6 8 0,000206 357527382 Maai maleylacetoacetate isomerase isoform 2 - 8,7 1 1 0,9992 14,9 2 2 0,9998 15,5 2 4 1 23,6 3 7 0,000322 62234487 ATP2B1 plasma membrane calcium ATPase 1 - 0 0 0 0,9992 12,2 14 2 0 0 0 0 0,9992 12,2 14 2 0,0000155 87196519 Wfdc10 WAP four-disulfide core domain 10 precursor - 8,1 1 1 0,9992 8,1 1 3 0,9851 8,1 1 1 0,9995 8,1 1 5 0,000738 110625794 SLC22A16 solute carrier family 22 member 16 - 1,8 1 1 0,9991 4 2 2 0,9997 4 2 2 0,9998 4 2 5 0,000083 13384742 Gm10078 28S ribosomal protein S36, mitochondrial isoform 1 - 0 0 0 0,9991 23,5 1 2 1 37,3 3 4 1 57,8 4 7 0,000829 148747177 RAB22A ras-related protein Rab-22A - 0 0 0 0,9991 9,8 2 2 0,9996 16 2 2 1 25,8 4 4 0,000115 166795271 4921517L17Rik cyclic nucleotide-binding domain-containing protein 2 - 1,3 1 1 0,9991 4,2 2 2 0 0 0 0 0,9995 4,2 2 3 0,0000303 169234938 ABCB7 ATP-binding cassette sub-family B member 7, mitochondrial - 0 0 0 0,9991 2,7 2 2 0 0 0 0 0,9991 2,7 2 2 0,0000238 19526994 LOC100047915 protein MEMO1 - 0 0 0 0,9991 6,7 2 2 0,9997 6,7 2 2 0,9997 6,7 2 4 0,000109 242247071 EFCAB10 EF-hand calcium-binding domain-containing protein 10 - 6,8 1 1 0,9991 12,1 2 2 0,9684 5,3 1 1 0,9992 12,1 2 4 0,000257 294862280 Ak4 GTP:AMP phosphotransferase AK4, mitochondrial - 0 0 0 0,9991 9 2 2 0 0 0 0 1 14,8 3 3 0,000106 38678526 NUP188 nucleoporin NUP188 homolog - 0 0 0 0,9991 1,3 2 2 0,9774 0,7 1 1 1 1,9 3 4 0,0000265 188497650 Mlec malectin precursor - 4,5 1 1 0,999 8,6 2 2 0 0 0 0 0,9993 8,6 2 3 0,0000691 18875380 rac3 ras-related C3 botulinum toxin substrate 3 precursor - 0 0 0 0,999 15,1 3 3 0,9724 4,2 1 2 0,999 15,1 3 4 0,000161 21312546 Fam162a protein FAM162A - 0 0 0 0,999 7,7 2 2 1 18,7 3 4 1 18,7 3 6 0,000349

21539587 NDUFA3 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 - 13,1 1 1 0,999 21,4 2 4 1 50 3 4 1 50 3 9 0,00100168 226442937 IQCF1 IQ domain-containing protein F1 isoform 1 - 0 0 0 0,999 18,1 2 2 0,9764 10,9 1 1 1 26,9 3 3 0,000149 G-protein coupled receptor family C group 5 member B isoform 2 23956118 Gprc5b precursor - 2,9 1 1 0,999 5,9 2 2 1 5,9 2 3 1 5,9 2 6 0,000339 6680988 Gm7795 cytochrome c oxidase subunit 6A1, mitochondrial - 14,3 1 2 0,999 14,3 1 3 0,9996 40,2 2 3 1 40,2 2 9 0,00143545 6755714 TAGLN transgelin - 0 0 0 0,999 9 2 2 1 19,4 4 8 1 23,9 5 10 0,000309 225637487 ANPEP aminopeptidase N - 0 0 0 0,9989 2,7 2 2 0 0 0 0 0,9989 2,7 2 2 0,0000226 119508441 Psmd9 26S proteasome non-ATPase regulatory subunit 9 - 3,6 1 1 0,9988 7,7 2 2 0,963 4,5 1 1 1 12,2 3 4 0,000152

13385012 Gm9803 mitochondrial import inner membrane translocase subunit TIM16 - 10,4 1 1 0,9988 30,4 2 2 0,9793 10,4 1 1 1 40,8 3 4 0,000202 CDGSH iron-sulfur domain-containing protein 3, mitochondrial 168229148 Cisd3 precursor - 0 0 0 0,9988 15,3 2 2 0 0 0 0 0 0 2 0 - 45504359 ATP6V1E1 V-type proton ATPase subunit E 1 - 0 0 0 0,9988 8,8 2 2 0 0 0 0 0,9987 8,8 2 2 0,0000597 54261793 atp2b4 plasma membrane calcium ATPase 4 isoform b - 25,1 23 1 0,9987 25,8 25 2 1 27,7 25 62 1 28,4 26 153 0,00137247 13385862 JAGN1 protein jagunal homolog 1 isoform 1 - 0 0 0 0,9986 17,9 1 2 0 0 0 0 0,999 17,9 1 2 0,000166 268370283 Gm5429 uncharacterized protein C7orf72 homolog - 0 0 0 0,9986 18,1 2 2 1 39,6 4 7 1 39,6 4 10 0,000727 229577332 Dnajb2 dnaJ homolog subfamily B member 2 isoform 3 - 0 0 0 0,9984 12,3 3 2 0 0 0 0 1 14,8 4 4 0,000103

6680690 prdx3 thioredoxin-dependent peroxide reductase, mitochondrial precursor - 0 0 0 0,9984 4,3 1 2 0,9996 10,1 2 2 1 14,4 3 4 0,000139 119392079 LOC100044248 uncharacterized protein C12orf60 homolog - 0 0 0 0,9982 8,5 2 2 1 18,6 4 5 1 18,6 4 7 0,000203 241666436 Ribc2 RIB43A-like with coiled-coils protein 2 - 0 0 0 0,9982 5,3 2 2 1 38,5 13 16 1 38,5 13 18 0,000329 124339826 Hspa1l heat shock 70 kDa protein 1B - 0 0 0 0,9953 15,3 8 19 0 0 0 0 0,9997 20 10 50 0,000578 14192922 Actc1 actin, alpha cardiac muscle 1 - 0 0 0 0,991 18,1 6 16 1 35,8 11 6 1 35,8 11 7 0,000119 100818178 KDM2A lysine-specific demethylase 2A - 0 0 0 - 0 0 0 1 5,6 5 8 1 6,4 6 9 0,0000601 calcium-binding tyrosine phosphorylation-regulated protein isoform 109255218 Cabyr c - 0 0 0 - 0 0 0 1 33,6 10 3 1 36 11 3 0,0000822 10946640 LOC100048611 ran guanine nucleotide release factor - 0 0 0 - 0 0 0 1 21,6 3 3 1 21,6 3 4 0,000332 Réplica 1 Réplica 2 Réplica 3 Datos combinados

124486652 COQ10A coenzyme Q-binding protein COQ10 homolog A, mitochondrial - 0 0 0 - 0 0 0 1 11,6 3 4 1 11,6 3 4 0,000109 126116585 KRT1 keratin, type II cytoskeletal 1 - 3,6 2 1 - 0 0 0 1 3,8 3 18 0,9999 3,8 3 35 0,000726 12963799 ATP6V1D V-type proton ATPase subunit D - 4,9 1 1 - 0 0 0 1 15,8 3 3 1 15,8 3 4 0,000115 13385268 CYB5 cytochrome b5 - 15,7 1 1 - 0 0 0 1 34,3 3 3 1 34,3 3 3 0,000201 13385270 HINT3 histidine triad nucleotide-binding protein 3 - 6,1 1 1 - 0 0 0 1 20 3 3 1 20 3 4 0,000218 13385654 4921536K21Rik uncharacterized protein C5orf52 homolog - 7,7 1 1 - 0 0 0 1 27,4 4 7 1 27,4 4 8 0,000319 13385812 PRSS37 probable inactive serine protease 37 precursor - 0 0 0 - 5,1 1 1 1 17,7 3 3 1 17,7 3 4 0,000147 13386034 rps13 40S ribosomal protein S13 - 7,9 1 1 - 0 0 0 1 24,5 3 3 1 24,5 3 4 0,00015 133922575 NUDCD2 nudC domain-containing protein 2 - 0 0 0 - 0 0 0 1 34,4 4 4 1 34,4 4 4 0,000201

13937391 gnb2 guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 - 0 0 0 - 0 0 0 1 9,1 3 3 1 12,6 4 4 0,0000983 NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial 140969946 NDUFV3 isoform 2 - 10,6 1 1 - 10,6 1 1 1 30,8 2 3 1 30,8 2 5 0,000295 148225760 Gm595 uncharacterized protein LOC209005 - 0 0 0 - 0 0 0 1 6,6 3 3 1 6,6 3 3 0,0000337 156255157 Acad10 acyl-CoA dehydrogenase family member 10 - 2,7 1 1 - 0 0 0 1 5,7 5 6 1 5,7 5 7 0,0000524 158966704 LOC100047501 40S ribosomal protein S16 - 0 0 0 - 5,5 1 1 1 22,6 4 4 1 22,6 4 6 0,00023 160333193 PECI enoyl-CoA delta isomerase 2, mitochondrial isoform a - 0 0 0 - 0 0 0 1 9,2 3 3 1 9,2 3 4 0,000103 160358825 Apoh beta-2-glycoprotein 1 precursor - 0 0 0 - 0 0 0 1 19,4 5 5 1 19,4 5 5 0,0000995 176865892 EIF4A2 eukaryotic initiation factor 4A-II isoform a - 3,2 1 1 - 0 0 0 1 11 3 4 1 11 3 5 0,0000938 19705578 atp6v1b2 V-type proton ATPase subunit B, brain isoform - 0 0 0 - 0 0 0 1 7,4 3 3 1 7,4 3 3 0,000046 198278559 1700013F07Rik uncharacterized protein C1orf194 homolog - 0 0 0 - 10,7 1 1 1 29,2 3 8 1 29,2 3 8 0,000311 21311849 Ggct gamma-glutamylcyclotransferase - 0 0 0 - 6,9 1 1 1 17,6 3 3 1 17,6 3 4 0,000121

21312062 tmed10 transmembrane emp24 domain-containing protein 10 precursor - 0 0 0 - 0 0 0 1 12,8 2 4 1 12,8 2 4 0,000171 21312520 QDPR dihydropteridine reductase - 0 0 0 - 0 0 0 1 14,1 3 3 1 14,1 3 3 0,0000927 21624609 BPHL valacyclovir hydrolase precursor - 0 0 0 - 0 0 0 1 18,6 4 4 1 18,6 4 4 0,0000885 21703810 Ubl4 ubiquitin-like protein 4A - 5,1 1 1 - 0 0 0 1 21,7 3 3 1 21,7 3 4 0,000161 21735489 C030006K11Rik UPF0598 protein C8orf82 homolog isoform 1 - 0 0 0 - 0 0 0 1 22 3 3 1 22 3 4 0,000136 22164770 Oit1 protein FAM3D precursor - 0 0 0 - 0 0 0 1 13 3 3 1 13 3 3 0,0000947 22164776 KRT79 keratin, type II cytoskeletal 79 - 2,3 1 4 - 0 0 0 1 8,1 4 3 1 8,1 4 7 0,000111 delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial 225543103 ALDH4A1 precursor - 0 0 0 - 0 0 0 1 13,5 6 7 1 10,7 5 6 0,0000916 225579132 Txndc2 thioredoxin domain-containing protein 2 isoform 2 - 0 0 0 - 2 1 1 1 10,3 3 3 1 10,3 3 5 0,0000731 225735584 HK1 hexokinase-1 isoform HK1 - 46,3 38 1 - 43,8 35 1 1 51,4 40 238 1 51,4 40 575 0,00356583 226442740 Sept10 septin-10 isoform 1 - 2,8 1 1 - 2,8 1 1 1 25,8 7 8 1 25,8 7 10 0,000174 226453478 COX2 cytochrome c oxidase subunit II [Mus musculus castaneus] - 0 0 0 - 0 0 0 1 40,5 5 41 1 60,8 7 76 0,00801545 226958354 PPM1B protein phosphatase 1B isoform 1 - 4,6 1 1 - 0 0 0 1 15,1 4 5 1 15,1 4 6 0,0000986 succinate dehydrogenase cytochrome b560 subunit, mitochondrial 228008307 sdhC precursor - 0 0 0 - 4,7 1 3 1 22,5 3 4 0,9995 12,4 2 6 0,00043 very long-chain specific acyl-CoA dehydrogenase, mitochondrial 23956084 ACADVL precursor - 0 0 0 - 0 0 0 1 6,7 3 3 1 6,7 3 3 0,0000315 240120058 4930544G11Rik ras homolog gene family, member A-like - 0 0 0 - 0 0 0 1 26,4 4 8 0,95 26,4 4 1 - 25092662 vps25 vacuolar protein-sorting-associated protein 25 - 0 0 0 - 0 0 0 1 27,3 5 7 1 27,3 5 7 0,000281 254553418 1700019O17Rik uncharacterized protein C2orf57 homolog - 0 0 0 - 3,4 1 1 1 7,4 3 5 1 7,4 3 6 0,000208 254587970 Cst12 cystatin-12 - 0 0 0 - 0 0 0 1 18,4 2 3 1 18,4 2 3 0,000133 254587975 SATL1 spermidine/spermine N(1)-acetyltransferase-like protein 1 - 0 0 0 - 1,5 1 2 1 5 3 7 1 5 3 9 0,000155

254588006 LRGUK leucine-rich repeat and guanylate kinase domain-containing protein - 0,9 1 1 - 0,9 1 1 1 7,8 4 4 1 8,7 5 6 0,0000744 electron transfer flavoprotein-ubiquinone oxidoreductase, 254588014 Etfdh mitochondrial precursor - 0 0 0 - 0 0 0 1 6,8 4 4 1 6,8 4 4 0,0000516 255760052 COPS7A COP9 signalosome complex subunit 7a isoform 2 - 0 0 0 - 0 0 0 1 12,7 3 3 1 12,7 3 3 0,0000909

262050625 Ssr4 translocon-associated protein subunit delta isoform 1 precursor - 6,4 1 1 - 0 0 0 1 30,8 4 5 1 30,8 4 6 0,000404 262359885 eif5a eukaryotic translation initiation factor 5A-1 - 0 0 0 - 0 0 0 1 28,6 3 4 1 28,6 3 4 0,00025 ATP-binding cassette sub-family B member 8, mitochondrial 27753995 abcb8 precursor - 1,8 1 1 - 0 0 0 1 7,7 3 3 0,9997 4,6 2 3 0,0000354 284172505 1700007I08Rik actin-like protein 10 - 0 0 0 - 0 0 0 1 34,1 7 8 1 34,1 7 8 0,000218 29126205 ACAA2 3-ketoacyl-CoA thiolase, mitochondrial - 3,5 1 1 - 0 0 0 1 23,7 6 6 1 23,7 6 7 0,000144 306966148 Smpd5 sphingomyelin phosphodiesterase 5 - 2,3 1 1 - 0 0 0 1 9,7 3 4 1 9,7 3 5 0,000127 307691260 - uncharacterized protein LOC627607 - 2,5 1 1 - 0 0 0 1 8,8 3 3 1 8,8 3 4 0,0000671 309265504 Rsph10a PREDICTED: radial spoke head 10 homolog B - 0 0 0 - 1,5 1 1 1 3,8 3 5 1 3,8 3 6 0,0000636 312222726 Angel1 protein angel homolog 2 isoform 2 - 1,9 1 1 - 0 0 0 1 9,6 4 4 1 9,6 4 5 0,0000828 Réplica 1 Réplica 2 Réplica 3 Datos combinados

31981784 9030617O03Rik UPF0317 protein C14orf159 homolog, mitochondrial precursor - 1,6 1 1 - 0 0 0 1 18,8 9 9 1 18,8 9 10 0,000129 31982133 AMDHD2 putative N-acetylglucosamine-6-phosphate deacetylase - 0 0 0 - 0 0 0 1 22 6 7 1 22 6 7 0,00017 34850748 MORN2 MORN repeat-containing protein 2 - 0 0 0 - 0 0 0 1 39,2 2 4 1 39,2 2 4 0,000344 39841061 4922502D21Rik C-type lectin-like receptor 2m - 4,2 1 3 - 0 0 0 1 14,3 3 5 1 14,3 3 8 0,000327 407261373 Ptges3 PREDICTED: prostaglandin E synthase 3-like - 0 0 0 - 0 0 0 1 26,2 3 5 1 26,2 3 5 0,000223 PREDICTED: membrane-spanning 4-domains subfamily A 407264262 Ms4a6b member 14 - 0 0 0 - 0 0 0 1 8 4 6 0 0 6 0 - 485050188 - predicted gene 11780 - 0 0 0 - 0 0 0 1 15,2 3 3 1 15,2 3 3 0,000132 50979268 1700065I17Rik uncharacterized protein C5orf48 homolog - 7,8 1 1 - 0 0 0 1 19,1 3 6 1 19,1 3 8 0,000536 52138554 LOC100045772 multidrug resistance-associated protein 9 - 0 0 0 - 0,6 1 1 1 4,8 4 4 1 5,4 5 5 0,0000378 54607171 KRT6A keratin, type II cytoskeletal 6A - 0 0 0 - 0 0 0 1 9,9 6 10 1 9,9 6 4 0,0000721 56961629 SPATA24 spermatogenesis-associated protein 24 isoform 1 - 0 0 0 - 0 0 0 1 20 3 4 1 20 3 5 0,000197 58037325 Dusp21 dual specificity phosphatase 21 - 7,9 1 1 - 0 0 0 1 13,8 2 4 1 13,8 2 5 0,00055 6679443 PPM1A protein phosphatase 1A - 2,4 1 1 - 0 0 0 1 21,7 6 7 1 21,7 6 8 0,000149 6679505 PSMD4 26S proteasome non-ATPase regulatory subunit 4 - 0 0 0 - 0 0 0 1 11,7 3 3 1 11,7 3 3 0,0000907 6680604 Krt16 keratin, type I cytoskeletal 16 - 5,8 3 1 - 0 0 0 1 11,7 5 3 1 14,1 6 4 0,000071 6753490 COPS4 COP9 signalosome complex subunit 4 - 0 0 0 - 0 0 0 1 14,5 4 4 1 14,5 4 4 0,0000747 6755000 pdcd6 programmed cell death protein 6 - 6,8 1 1 - 0 0 0 1 16,8 3 3 1 16,8 3 4 0,000175 7305339 Odf2 outer dense fiber protein 2 isoform c - 0 0 0 - 0 0 0 1 46,6 27 214 0,9853 48 30 3 - serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit 8394027 PPP2R1A A alpha isoform - 1,7 1 2 - 0 0 0 1 10,7 5 7 1 10,7 5 10 0,000134 85701852 Gm614 uncharacterized protein CXorf65 homolog - 5,8 1 1 - 0 0 0 1 30,4 6 9 1 30,4 6 10 0,000314 85702101 Akap14 A-kinase anchor protein 14 - 0 0 0 - 0 0 0 1 9,3 5 7 1 9,3 5 7 0,0000992 86198335 NUDT2 bis(5'-nucleosyl)-tetraphosphatase [asymmetrical] - 0 0 0 - 0 0 0 1 32 4 4 1 32 4 4 0,000184 88759352 glyctk glycerate kinase - 0 0 0 - 0 0 0 1 10,7 4 5 1 10,7 4 5 0,000116 90017457 FDX1L adrenodoxin-like protein, mitochondrial precursor - 0 0 0 - 0 0 0 1 23,6 3 3 0,9995 16,7 2 2 0,0000818 9789985 ivd isovaleryl-CoA dehydrogenase, mitochondrial precursor - 2,8 1 1 - 0 0 0 1 7,8 3 3 1 7,8 3 4 0,0000708 9790219 DSTN destrin - 0 0 0 - 0 0 0 1 38,8 5 5 1 38,8 5 5 0,000134 9910550 SEC11A signal peptidase complex catalytic subunit SEC11A - 4,5 1 2 - 0 0 0 1 15,6 3 4 1 15,6 3 7 0,000246 11230802 actn4 alpha-actinin-4 - 0 0 0 - 0 0 0 0,9998 2,1 2 2 0,9998 2,1 2 2 0,000016 114158691 Cops8 COP9 signalosome complex subunit 8 - 6,7 1 1 - 0 0 0 0,9998 12,9 2 2 0,9997 12,9 2 3 0,000174 125347370 TEX13A testis-expressed sequence 13A protein - 0 0 0 - 0 0 0 0,9998 6,6 2 2 0,9998 6,6 2 2 0,0000472 128485805 pbp2 phosphatidylethanolamine-binding protein 2 - 0 0 0 - 0 0 0 0,9998 18,2 2 3 0,9998 18,2 2 3 0,000165 Tabla suplementaria 10. Fenotipos reproductivos asociados con proteinas identificadas en los análisis de espectrometría de masas. La información viene dada en inglés porque fue recogida de la base de datos de Mouse Genomics Informatics.

Protein Symbol Protein name Term abnormal sperm end piece morphology abnormal sperm flagellum morphology abnormal sperm mitochondrial sheath morphology abnormal sperm physiology Akap4 A kinase (PRKA) anchor protein 4 abnormal sperm principal piece morphology male infertility reduced activated sperm motility reduced hyperactivated sperm motility abnormal spermatogenesis abnormal sperm flagellum morphology abnormal sperm head morphology abnormal sperm physiology Prss21 protease, serine 21 asthenozoospermia decreased fertilization frequency impaired acrosome reaction impaired fertilization abnormal spermatid morphology abnormal sperm flagellum morphology Nme5 NME/NM23 family member 5 arrest of spermatogenesis azoospermia abnormal sperm flagellum morphology abnormal sperm midpiece morphology abnormal sperm physiology Tekt4 tektin 4 abnormal sperm principal piece morphology asthenozoospermia reduced male fertility abnormal male germ cell apoptosis abnormal spermatid morphology abnormal spermatocyte morphology Hspa4 heat shock protein 4 abnormal spermatogenesis arrest of male meiosis asthenozoospermia male infertility abnormal spermatid morphology abnormal spermatogenesis arrest of spermatogenesis Tssk2 testis-specific serine kinase 2 asthenozoospermia azoospermia male infertility abnormal spermatid morphology abnormal spermatogenesis arrest of spermatogenesis Tssk1 testis-specific serine kinase 1 asthenozoospermia azoospermia male infertility impaired acrosome reaction Mfge8 milk fat globule-EGF factor 8 protein reduced male fertility a disintegrin and metallopeptidase asthenozoospermia Adam1a domain 1a Protein Symbol aProtein disintegrin name and metallopeptidase Term Adam1a domain 1a male infertility abnormal spermatogenesis arrest of male meiosis Bsg basigin azoospermia male infertility a disintegrin and metallopeptidase asthenozoospermia Adam2 domain 2 reduced male fertility abnormal sperm physiology asthenozoospermia potassium channel, subfamily U, member impaired acrosome reaction Kcnu1 1 impaired fertilization impaired sperm capacitation male infertility abnormal sperm physiology Spam1 sperm adhesion molecule 1 impaired fertilization impaired acrosome reaction a disintegrin and metallopeptidase Adam3 impaired fertilization domain 3 (cyritestin) male infertility abnormal sperm motility abnormal sperm physiology asthenozoospermia Catsper2 cation channel, sperm associated 2 impaired acrosome reaction impaired fertilization male infertility abnormal sperm physiology Plcd4 phospholipase C, delta 4 impaired acrosome reaction male infertility abnormal male reproductive system physiology radial spoke head 1 homolog abnormal spermatid morphology Rsph1 (Chlamydomonas) abnormal sperm physiology male infertility abnormal sperm physiology Spesp1 sperm equatorial segment protein 1 decreased fertilization frequency abnormal spermiogenesis Klhl10 kelch-like 10 arrest of spermatogenesis male infertility abnormal sperm motility protein kinase, cAMP dependent, asthenozoospermia Prkaca catalytic, alpha impaired fertilization male infertility abnormal spermatogenesis abnormal sperm head morphology Tssk6 testis-specific serine kinase 6 asthenozoospermia male infertility abnormal sperm axoneme morphology Tekt2 tektin 2 asthenozoospermia male infertility abnormal sperm head morphology asthenozoospermia sperm mitochondria-associated cysteine- impaired acrosome reaction Smcp rich protein impaired fertilization sperm mitochondria-associated cysteine- Smcp Protein Symbol richProtein protein name Term male infertility reduced male fertility Car2 carbonic anhydrase 2 reduced male fertility Crisp1 cysteine-rich secretory protein 1 abnormal sperm physiology Crisp4 cysteine-rich secretory protein 4 impaired acrosome reaction abnormal male germ cell morphology abnormal spermiogenesis capping protein (actin filament) muscle Z- Capza3 abnormal sperm motility line, alpha 3 abnormal sperm number male infertility asthenozoospermia decreased fertilization frequency transient receptor potential cation Trpv6 impaired fertilization channel, subfamily V, member 6 reduced fertility reduced male fertility abnormal sperm midpiece morphology abnormal sperm mitochondrial sheath morphology Odf1 outer dense fiber of sperm tails 1 asthenozoospermia impaired acrosome reaction male infertility impaired fertilization Mmel1 membrane metallo-endopeptidase-like 1 male infertility reduced male fertility arrest of male meiosis Gnpat glyceronephosphate O-acyltransferase azoospermia male infertility Ank1 ankyrin 1, erythroid infertility arrest of male meiosis Lmna lamin A azoospermia infertility abnormal sperm nucleus morphology Gpx5 glutathione peroxidase 5 reduced male fertility Acrbp proacrosin binding protein impaired fertilization abnormal sperm motility abnormal sperm physiology Catsper4 cation channel, sperm associated 4 asthenozoospermia impaired sperm capacitation male infertility Spta1 spectrin alpha, erythrocytic 1 infertility abnormal sperm motility Chdh choline dehydrogenase reduced male fertility abnormal spermatogenesis abnormal sperm head morphology Tssk6 testis-specific serine kinase 6 asthenozoospermia male infertility abnormal spermatid morphology abnormal spermatogenesis abnormal sperm flagellum morphology Spaca1 sperm acrosome associated 1 abnormal sperm head morphology asthenozoospermia male infertility Protein Symbol Protein name Term abnormal sperm motility abnormal sperm physiology asthenozoospermia Catsper1 cation channel, sperm associated 1 impaired acrosome reaction impaired fertilization male infertility abnormal sperm physiology asthenozoospermia ATPase, Na+/K+ transporting, alpha 4 Atp1a4 impaired fertilization polypeptide impaired sperm capacitation male infertility glycerol phosphate dehydrogenase 2, Gpd2 mitochondrial reduced fertility abnormal spermatid morphology abnormal spermatogenesis abnormal spermiogenesis Hook1 hook homolog 1 (Drosophila) abnormal sperm nucleus morphology absent sperm flagellum arrest of spermatogenesis reduced male fertility asthenozoospermia Pgk2 phosphoglycerate kinase 2 male infertility reduced male fertility

Ptgs2 prostaglandin-endoperoxide synthase 2 impaired fertilization abnormal sperm physiology asthenozoospermia ATPase, Na+/K+ transporting, alpha 4 Atp1a4 impaired fertilization polypeptide impaired sperm capacitation male infertility protein disulfide isomerase-like, testis abnormal sperm physiology Pdilt expressed male infertility abnormal sperm midpiece morphology asthenozoospermia Nup210l nucleoporin 210-like azoospermia male infertility abnormal sperm head morphology arrest of spermatogenesis Ak7 adenylate kinase 7 azoospermia male infertility abnormal male germ cell apoptosis solute carrier family 25 (mitochondrial abnormal spermatocyte morphology Slc25a31 carrier; adenine nucleotide translocator), arrest of male meiosis member 31 male infertility Zan zonadhesin abnormal sperm physiology asthenozoospermia Dnah1 dynein, axonemal, heavy chain 1 male infertility abnormal germ cell morphology Catsperd catsper channel auxiliary subunit delta male infertility reduced hyperactivated sperm motility abnormal spermatid morphology

Zpbp2 zona pellucida binding protein 2 Protein Symbol Protein name Term abnormal spermatogenesis abnormal sperm flagellum morphology Zpbp2 zona pellucida binding protein 2 abnormal sperm nucleus morphology asthenozoospermia male infertility reduced male fertility abnormal sperm physiology ATPase, Ca++ transporting, plasma Atp2b4 asthenozoospermia membrane 4 male infertility abnormal sperm midpiece morphology abnormal sperm mitochondrial sheath morphology Odf1 outer dense fiber of sperm tails 1 asthenozoospermia impaired acrosome reaction male infertility abnormal sperm axoneme morphology abnormal sperm flagellum morphology abnormal sperm midpiece morphology Odf2 outer dense fiber of sperm tails 2 asthenozoospermia male infertility reduced male fertility abnormal spermatid morphology abnormal spermatogenesis abnormal sperm flagellum morphology Zpbp zona pellucida binding protein abnormal sperm nucleus morphology asthenozoospermia male infertility reduced male fertility protein kinase, cAMP dependent infertility Prkar1a regulatory, type I, alpha reduced male fertility abnormal male meiosis Hspa2 heat shock protein 2 arrest of spermatogenesis male infertility Hspa1a heat shock protein 1A abnormal spermatocyte morphology Hspa1b heat shock protein 1B abnormal spermatocyte morphology abnormal spermatogenesis IMP2 inner mitochondrial membrane Immp2l male infertility peptidase-like (S. cerevisiae) reduced fertility abnormal male germ cell morphology abnormal male reproductive system morphology hydroxysteroid (17-beta) dehydrogenase abnormal spermatid morphology Hsd17b4 4 abnormal spermatogenesis male infertility reduced male fertility abnormal male reproductive system physiology angiotensin I converting enzyme Ace impaired fertilization (peptidyl-dipeptidase A) 1 reduced male fertility abnormal spermatid morphology abnormal sperm axoneme morphology abnormal sperm flagellum morphology abnormal sperm nucleus morphology Theg testicular haploid expressed gene absent sperm flagellum Theg testicular haploid expressed gene Protein Symbol Protein name Term asthenozoospermia azoospermia male infertility abnormal spermatogenesis abnormal spermiation Hspa4l heat shock protein 4 like asthenozoospermia reduced male fertility Fabp9 fatty acid binding protein 9, testis abnormal sperm head morphology impaired acrosome reaction Atp8b3 ATPase, class I, type 8B, member 3 reduced male fertility HYDIN, axonemal central pair apparatus Hydin protein male infertility abnormal sperm head morphology asthenozoospermia Acr acrosin prepropeptide impaired fertilization male infertility reduced male fertility abnormal sperm motility protein kinase, cAMP dependent, asthenozoospermia Prkaca catalytic, alpha impaired fertilization male infertility asthenozoospermia Tekt3 tektin 3 reduced male fertility impaired fertilization Izumo1 izumo sperm-egg fusion 1 male infertility abnormal spermatid morphology abnormal sperm axoneme morphology abnormal sperm flagellum morphology meiosis-specific nuclear structural Mns1 abnormal sperm mitochondrial sheath morphology protein 1 abnormal sperm principal piece morphology asthenozoospermia male infertility glyceraldehyde-3-phosphate asthenozoospermia Gapdhs dehydrogenase, spermatogenic male infertility abnormal spermatid morphology Krt19 keratin 19 abnormal sperm flagellum morphology abnormal male germ cell apoptosis abnormal spermatid morphology abnormal sperm head morphology Csnk2a2 casein kinase 2, alpha prime polypeptide abnormal sperm motility abnormal sperm nucleus morphology male infertility abnormal sperm motility Cyct cytochrome c, testis impaired fertilization abnormal male germ cell apoptosis abnormal male meiosis heat shock protein 90, alpha (cytosolic), arrest of male meiosis Hsp90aa1 class A member 1 arrest of spermatogenesis azoospermia male infertility Gm128 predicted gene 128 decreased fertilization frequency Protein Symbol Protein name Term abnormal spermatid morphology abnormal spermatogenesis Npepps aminopeptidase puromycin sensitive asthenozoospermia male infertility reduced fertility abnormal male germ cell apoptosis abnormal spermatogenesis abnormal sperm axoneme morphology Spag16 sperm associated antigen 16 abnormal sperm motility arrest of spermatogenesis asthenozoospermia male infertility abnormal male germ cell apoptosis abnormal male germ cell morphology abnormal spermatogenesis Insl6 insulin-like 6 asthenozoospermia azoospermia male infertility reduced male fertility abnormal sperm physiology asthenozoospermia Ldhc lactate dehydrogenase C impaired fertilization male infertility reduced male fertility abnormal sperm axoneme morphology abnormal sperm flagellum morphology abnormal sperm midpiece morphology Spag6 sperm associated antigen 6 abnormal sperm principal piece morphology absent sperm head asthenozoospermia male infertility Sptb spectrin beta, erythrocytic infertility abnormal spermatid morphology abnormal spermiogenesis asthenozoospermia Lipe lipase, hormone sensitive azoospermia male infertility reduced male fertility abnormal sperm motility abnormal sperm physiology Tex101 testis expressed gene 101 impaired acrosome reaction male infertility Rho GDP dissociation inhibitor (GDI) impaired fertilization Arhgdia alpha male infertility Aqp7 aquaporin 7 abnormal sperm physiology a disintegrin and metallopeptidase Adam24 domain 24 (testase 1) reduced male fertility abnormal spermatogenesis Tk1 thymidine kinase 1 reduced fertility abnormal spermatogenesis absent sperm head Gpr64 G protein-coupled receptor 64 Protein Symbol Protein name Term Gpr64 G protein-coupled receptor 64 asthenozoospermia reduced male fertility abnormal sperm axoneme morphology tubulin polyglutamylase complex subunit abnormal spermiogenesis Tpgs1 1 absent sperm flagellum male infertility abnormal sperm motility abnormal sperm physiology Catsper3 cation channel, sperm associated 3 asthenozoospermia impaired sperm capacitation male infertility abnormal male germ cell apoptosis abnormal male germ cell morphology abnormal sperm head morphology Cd59b CD59b antigen absent sperm head asthenozoospermia reduced male fertility serine (or cysteine) peptidase inhibitor, Serpine2 clade E, member 2 male infertility abnormal spermatogenesis abnormal spermiogenesis calcium and integrin binding 1 Cib1 arrest of spermatogenesis (calmyrin) azoospermia male infertility Hist1h1c histone cluster 1, H1c reduced fertility abnormal male reproductive system morphology abnormal sperm head morphology Fhl5 four and a half LIM domains 5 abnormal sperm nucleus morphology asthenozoospermia abnormal male germ cell apoptosis abnormal male reproductive system morphology Sycp2 synaptonemal complex protein 2 arrest of male meiosis azoospermia male infertility abnormal spermatogenesis Bcl2l1 BCL2-like 1 azoospermia male infertility Cav1 caveolin 1, caveolae protein reduced fertility abnormal sperm number Dkkl1 dickkopf-like 1 abnormal sperm physiology decreased fertilization frequency abnormal male reproductive system morphology abnormal spermatid morphology abnormal sperm flagellum morphology calcium/calmodulin-dependent protein Camk4 abnormal sperm head morphology kinase IV abnormal spermiogenesis arrest of spermatogenesis male infertility

phospholipid hydroperoxide glutathione Gpx4 male infertility peroxidase, nuclear isoform 1 phospholipid hydroperoxide glutathione Gpx4Protein Symbol Protein name Term peroxidase, nuclear isoform 1 Impaired sperm quality abnormal sperm flagellum morphology Tabla suplementaria 11. Proteínas detectadas exlusivamente en las especies.

Número de espectros Proteína Nombre de la proteína Especie Réplica 1 Réplica 2 Réplica 3 Combinación GNB2L1 guanine nucleotide-binding protein subunit beta-2-like 1 Mus musculus 3 0 2 6 FTH1 ferritin heavy chain Mus musculus 0 4 4 10 Gstz1 maleylacetoacetate isomerase isoform 1 Mus musculus 4 0 8 12 Hist1h1c histone H1.2 Mus musculus 2 0 4 4 ociad1 OCIA domain-containing protein 1 isoform 1 Mus musculus 2 2 2 6 OTUB2 ubiquitin thioesterase OTUB2 isoform 2 Mus musculus 3 0 2 6 dusp18 dual specificity protein phosphatase 18 Mus musculus 2 4 0 0 Gm6204 40S ribosomal protein S14 Mus musculus 2 0 3 8 B2M beta-2-microglobulin precursor Mus musculus 2 0 2 5 LOC633860 3(2),5-bisphosphate nucleotidase 1 Mus musculus 0 2 5 6 Gm7181 ubiquitin-conjugating enzyme E2 variant 1 Mus musculus 0 4 5 12 1700016G14Rik PREDICTED: uncharacterized protein LOC666145 Mus musculus 2 0 2 3 PMPCB mitochondrial-processing peptidase subunit beta precursor Mus musculus 2 4 3 9 MFGE8 lactadherin isoform 2 precursor Mus musculus 9 5 33 46 Gm12164 ferritin light chain 1 Mus musculus 0 2 2 4 TSKS testis-specific serine kinase substrate isoform 1 Mus musculus 4 0 12 15 acad8 isobutyryl-CoA dehydrogenase, mitochondrial precursor Mus musculus 4 4 11 22 SPINK5 serine protease inhibitor Kazal-type 5 precursor Mus musculus 3 0 7 11 Acot10 acyl-coenzyme A thioesterase 10, mitochondrial precursor Mus musculus 5 18 3 12 COL18A1 collagen alpha-1(XVIII) chain isoform 1 precursor Mus musculus 0 3 5 12 Akr1b7 aldose reductase-related protein 1 Mus musculus 0 3 6 10 IDH1 isocitrate dehydrogenase [NADP] cytoplasmic Mus musculus 2 6 6 14 AllC probable allantoicase Mus musculus 2 0 3 5 LMNB1 lamin-B1 Mus musculus 0 8 3 11 Gm15451 60S ribosomal protein L10a Mus musculus 2 0 2 4 Gpr64 epididymis-specific protein isoform 1 precursor Mus musculus 6 3 6 16 WWP2 NEDD4-like E3 ubiquitin-protein ligase WWP2 Mus spretus 3 0 2 6 Gm2904 S-formylglutathione hydrolase Mus spretus 0 2 2 5 TSFM elongation factor Ts, mitochondrial precursor Mus spretus 2 3 2 7 DKKL1 dickkopf-like protein 1 precursor Mus spretus 5 3 5 13 Gm6560 pyruvate kinase, muscle isoform M2 Mus spretus 3 6 5 14 FOLR1 folate receptor alpha precursor Mus spretus 2 0 2 5 hexA beta-hexosaminidase subunit alpha precursor Mus spretus 0 3 3 7 GSK3A glycogen synthase kinase-3 alpha Mus spretus 0 2 3 6 coenzyme Q-binding protein COQ10 homolog B, mitochondrial Gm4899 isoform 2 Mus spretus 0 2 3 5 GGH gamma-glutamyl hydrolase precursor Mus spretus 0 7 5 11 pnpla8 calcium-independent phospholipase A2-gamma Mus spretus 0 4 3 8 carcinoembryonic antigen-related cell adhesion molecule 2 Ceacam2 isoform a precursor Mus spretus 6 3 4 13 LETM1 domain-containing protein LETM2, mitochondrial LETM2 precursor Mus spretus 0 3 2 5 COX3 ATP synthase F0 subunit 8 [Mus musculus musculus] Mus spretus 0 4 4 11 1700029J11Rik uncharacterized protein LOC76426 precursor Mus spretus 2 0 2 4 Faah fatty-acid amide hydrolase 1 Mus spretus 3 3 2 8 G6pd2 glucose-6-phosphate 1-dehydrogenase 2 Mus spretus 8 4 7 15 Acot2 acyl-coenzyme A thioesterase 2, mitochondrial precursor Mus spretus 4 4 0 0 atad3a ATPase family AAA domain-containing protein 3 Mus spretus 6 2 0 7 1700011H22Rik izumo sperm-egg fusion protein 3 precursor Mus spretus 0 5 5 16 PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur Ndufs6 protein 6, mitochondrial-like Mus spretus 2 5 3 10 - prostate and testis expressed precursor Mus spretus 0 2 3 5 enoyl-CoA hydratase domain-containing protein 2, Echdc2 mitochondrial isoform 2 Mus spretus 6 6 9 21 GLG1 Golgi apparatus protein 1 precursor Mus spicilegus 3 3 0 6 clpB caseinolytic peptidase B protein homolog Mus spicilegus 2 0 4 6 slc30a1 zinc transporter 1 Mus spicilegus 5 3 4 12 Gm1974 translationally-controlled tumor protein Mus spicilegus 0 2 2 4 itm2b integral membrane protein 2B Mus spicilegus 0 2 3 5 AIFM1 apoptosis-inducing factor 1, mitochondrial precursor Mus spicilegus 2 3 0 6 Atp11a probable phospholipid-transporting ATPase IH Mus spicilegus 3 0 2 5 grhpr glyoxylate reductase/hydroxypyruvate reductase Mus spicilegus 2 2 0 5 LOC100047915 protein MEMO1 Mus spicilegus 0 2 2 4 Rab2b ras-related protein Rab-2B Mus spicilegus 0 2 2 5 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit Ndufa4 4 Mus spicilegus 0 2 2 5 Aldh3b1 aldehyde dehydrogenase family 3 member B1 Mus spicilegus 6 0 4 10 G protein-activated inward rectifier potassium channel 2 isoform Kcnj6 Girk2A-1 Mus spicilegus 2 0 2 4 Número de espectros Proteína Nombre de la proteína Especie Réplica 1 Réplica 2 Réplica 3 Combinación

LOC632398 malonyl-CoA-acyl carrier protein transacylase, mitochondrial Mus spicilegus 2 4 6 12 DCST2 PREDICTED: DC-STAMP domain-containing protein 2 Mus spicilegus 4 0 3 8 PREDICTED: NADH dehydrogenase [ubiquinone] 1 subunit C2- LOC675851 like Mus spicilegus 4 0 5 12 GLRX2 glutaredoxin-2, mitochondrial isoform b Mus spicilegus 0 2 2 4 SLC22A16 solute carrier family 22 member 16 Mus spicilegus 0 2 2 5 Dcst1 DC-STAMP domain-containing protein 1 Mus spicilegus 2 0 3 5 Enah protein enabled homolog isoform 1 Mus spicilegus 0 2 2 5 RABEP2 rab GTPase-binding effector protein 2 Mus spicilegus 0 2 2 4 MUT methylmalonyl-CoA mutase, mitochondrial precursor Mus spicilegus 0 2 4 6 LOC100047713 mitochondrial intermediate peptidase precursor Mus spicilegus 0 7 4 11 serine/threonine-protein phosphatase 4 regulatory subunit 1 Ppp4r1 isoform a Mus spicilegus 2 2 0 4 FCGBP Fc fragment of IgG binding protein precursor Mus spicilegus 0 5 4 9 Gm3448 t-complex-associated testis expressed 3-like Mus spicilegus 6 0 7 14 SIGLEC1 sialoadhesin precursor Mus spicilegus 0 12 14 26 SLC3A2 4F2 cell-surface antigen heavy chain isoform a Mus spicilegus 2 3 2 7 FAM183B protein FAM183B isoform 1 Mus spicilegus 2 0 3 5 1700061J05Rik testis-expressed sequence 33 protein isoform 1 Mus spicilegus 0 5 3 8 Tabla suplementaria 12. Análisis comparativos entre Mus musculus y Mus spretus. Las proteínas con diferencias significativas son indicadas en negrita.

Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia Irgc1 interferon-inducible GTPase 5 0,003177998 0,001088223 2,920 8,478 0 Mus musculus LOC100046120 clusterin precursor 0,003772178 7,03E-04 5,363 10,018 0 Mus musculus FCGBP Fc fragment of IgG binding protein-like precursor 4,59E-04 2,49E-05 18,416 9,582 0 Mus musculus atp2b4 plasma membrane calcium ATPase 4 isoform a 0,001607779 1,43E-04 11,277 12,830 0 Mus musculus Pcx pyruvate carboxylase, mitochondrial isoform 2 4,50E-04 3,06E-05 14,699 7,903 2,66E-15 Mus musculus FABP9 fatty acid-binding protein 9 0,004450144 0,00166101 2,679 7,131 9,99E-13 Mus musculus Spnb1 spectrin beta chain, erythrocytic 1,46E-04 1,11E-05 13,225 6,531 6,55E-11 Mus musculus Gm16486 PREDICTED: uncharacterized protein KIAA1683 5,08E-04 1,05E-04 4,855 6,383 1,74E-10 Mus musculus Pacrg parkin coregulated gene protein homolog 0,002466453 5,88E-04 4,191 6,365 1,96E-10 Mus musculus GSTM5 glutathione S-transferase Mu 5 0,003974018 0,002420019 1,642 5,875 4,22E-09 Mus musculus Spna1 spectrin alpha chain, erythrocytic 1 1,22E-04 1,07E-05 11,402 5,869 4,39E-09 Mus musculus SLC4A1 band 3 anion transport protein 0,001020103 2,01E-04 5,074 5,511 3,56E-08 Mus musculus phospholipid hydroperoxide glutathione peroxidase, hnrpll nuclear isoform 1 0,003446861 0,001711689 2,014 5,502 3,76E-08 Mus musculus PFKM 6-phosphofructokinase, muscle type 0,001031216 4,01E-04 2,573 5,258 1,45E-07 Mus musculus PGP phosphoglycolate phosphatase 8,18E-04 1,20E-04 6,794 5,141 2,73E-07 Mus musculus phospholipase B1, membrane-associated isoform 1 PLB1 precursor 5,26E-04 2,17E-04 2,423 5,000 5,72E-07 Mus musculus glycerol-3-phosphate dehydrogenase, mitochondrial Gpd2 precursor 0,00268629 0,001780131 1,509 4,981 6,33E-07 Mus musculus cytochrome c oxidase subunit 4 isoform 1, mitochondrial COX4I1 precursor 0,008421194 0,005429986 1,551 4,974 6,57E-07 Mus musculus Slc25a31 ADP/ATP translocase 4 0,003471802 0,002373479 1,463 4,934 8,04E-07 Mus musculus AKAP4 A-kinase anchor protein 4 isoform b 0,005859698 0,005202227 1,126 4,831 1,36E-06 Mus musculus KRT19 keratin, type I cytoskeletal 19 3,62E-04 2,63E-05 13,797 4,805 1,55E-06 Mus musculus Gm5604 keratin, type II cytoskeletal 8 4,08E-04 4,03E-05 10,133 4,788 1,69E-06 Mus musculus ACO2 aconitate hydratase, mitochondrial precursor 0,005409188 0,004188298 1,292 4,784 1,72E-06 Mus musculus HEATR7A HEAT repeat containing 7A isoform 1 2,08E-04 4,23E-05 4,912 4,629 3,68E-06 Mus musculus TPI1 triosephosphate isomerase 0,002633418 0,001421865 1,852 4,494 6,98E-06 Mus musculus BSPRY B box and SPRY domain-containing protein 0,001360709 5,68E-04 2,396 4,429 9,49E-06 Mus musculus DPEP3 dipeptidase 3 precursor 0,00255772 0,001421819 1,799 4,378 1,20E-05 Mus musculus vars valine--tRNA ligase 1,88E-04 3,55E-05 5,289 4,203 2,64E-05 Mus musculus vdac2 voltage-dependent anion-selective channel protein 2 0,00250379 0,00134069 1,868 4,177 2,95E-05 Mus musculus Hspa2 heat shock-related 70 kDa protein 2 0,001503172 8,39E-04 1,791 4,102 4,09E-05 Mus musculus IMMT mitochondrial inner membrane protein isoform 1 4,14E-04 0,003471768 8,394 -15,779 4,33E-56 Mus spretus zan zonadhesin precursor 4,37E-04 0,001237238 2,829 -13,052 6,23E-39 Mus spretus sorD sorbitol dehydrogenase 0,003430472 0,008220805 2,396 -8,909 5,16E-19 Mus spretus

ZPBP2 zona pellucida-binding protein 2 isoform 1 precursor 0,001085162 0,003500504 3,226 -6,750 1,48E-11 Mus spretus bifunctional ATP-dependent dihydroxyacetone dak kinase/FAD-AMP lyase (cyclizing) 6,69E-04 0,001997272 2,988 -6,401 1,55E-10 Mus spretus PGK2 phosphoglycerate kinase 2 0,003359474 0,00600817 1,788 -6,171 6,77E-10 Mus spretus PREDICTED: LOW QUALITY PROTEIN: dynein dnhc3 heavy chain 3, axonemal 4,01E-05 1,63E-04 4,068 -5,940 2,85E-09 Mus spretus succinyl-CoA ligase [ADP-forming] subunit beta, SUCLA2 mitochondrial precursor 0,004455064 0,006505705 1,460 -5,723 1,05E-08 Mus spretus cAMP-dependent protein kinase type II-alpha regulatory PRKAR2A subunit 0,003106839 0,005355296 1,724 -5,674 1,40E-08 Mus spretus

MECR trans-2-enoyl-CoA reductase, mitochondrial precursor 1,72E-04 0,001161125 6,759 -5,396 6,82E-08 Mus spretus SCAMP1 secretory carrier-associated membrane protein 1 9,61E-04 0,003365563 3,503 -5,163 2,43E-07 Mus spretus GLOD4 glyoxalase domain-containing protein 4 1,10E-04 7,70E-04 7,024 -4,869 1,12E-06 Mus spretus CD109 CD109 antigen precursor 2,36E-04 6,49E-04 2,753 -4,864 1,15E-06 Mus spretus MAN2B2 epididymis-specific alpha-mannosidase precursor 5,29E-04 0,001273011 2,408 -4,861 1,17E-06 Mus spretus

Gm4787 a disintegrin and metalloprotease domain 4b precursor 3,79E-05 4,00E-04 10,551 -4,815 1,47E-06 Mus spretus Gm884 uncharacterized protein LOC380730 8,07E-04 0,001421706 1,762 -4,598 4,27E-06 Mus spretus BC049730 uncharacterized protein LOC232972 precursor 1,57E-04 0,001393365 8,901 -4,468 7,91E-06 Mus spretus man2c1 alpha-mannosidase 2C1 2,11E-04 6,38E-04 3,022 -4,343 1,41E-05 Mus spretus Csl citrate synthase-like protein 0,004375624 0,006476897 1,480 -4,201 2,65E-05 Mus spretus Cd46 membrane cofactor protein precursor 4,98E-04 0,001608731 3,228 -4,191 2,78E-05 Mus spretus very long-chain specific acyl-CoA dehydrogenase, ACADVL mitochondrial precursor 2,65E-04 4,78E-05 5,541 4,042 5,31E-05 Rsph1 radial spoke head 1 homolog 0,002686207 0,001243148 2,161 4,036 5,45E-05 Slc25a21 mitochondrial 2-oxodicarboxylate carrier isoform 1 6,81E-04 1,65E-04 4,125 4,030 5,58E-05 GLIPR1L2 GLIPR1-like protein 2 7,43E-04 0,002062109 2,775 -3,976 7,01E-05 ACAT1 acetyl-CoA acetyltransferase, mitochondrial precursor 0,001886715 0,001027675 1,836 3,929 8,54E-05

#N/A PREDICTED: leucine-rich repeat-containing protein 37A2 5,73E-04 9,76E-04 1,703 -3,923 8,74E-05 FSCB fibrous sheath CABYR-binding protein 0,003323024 0,00234733 1,416 3,894 9,87E-05 ATP-binding cassette sub-family B member 8, mitochondrial abcb8 precursor 2,48E-04 3,13E-05 7,937 3,860 1,14E-04 succinyl-CoA:3-ketoacid coenzyme A transferase 2B, LOC100046138 mitochondrial precursor 0,007713328 0,009599666 1,245 -3,854 1,16E-04 Adam6a a disintegrin and metalloprotease domain 6 4,40E-04 1,43E-04 3,071 3,830 1,28E-04 succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, suclg1 mitochondrial precursor 0,006051398 0,00943364 1,559 -3,828 1,29E-04 4930519F16Rik uncharacterized protein LOC75106 precursor 2,99E-04 0,001443312 4,835 -3,798 1,46E-04 NUP210L nuclear pore membrane glycoprotein 210-like precursor 2,31E-04 8,65E-05 2,674 3,790 1,51E-04 Rsph3b radial spoke head protein 3 homolog B 0,001725308 8,81E-04 1,959 3,780 1,57E-04 Hspa4l heat shock 70 kDa protein 4L 2,11E-04 5,07E-04 2,399 -3,707 2,10E-04

Nsun4 PREDICTED: sperm head and tail associated protein-like 3,96E-04 8,53E-05 4,642 3,623 2,91E-04 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA12 subunit 12 0,002191033 8,33E-04 2,631 3,580 3,44E-04 Smcp sperm mitochondrial-associated cysteine-rich protein 0,003612009 0,001613096 2,239 3,571 3,56E-04 Ccdc114 coiled-coil domain-containing protein 114 5,00E-04 1,86E-04 2,683 3,571 3,56E-04 Fam166a protein FAM166A 0,004866493 0,003265506 1,490 3,568 3,60E-04 ODF1 outer dense fiber protein 1 0,002856277 0,001893666 1,508 3,489 4,85E-04 Wfdc16 WAP four-disulfide core domain 16 0,002312074 4,40E-04 5,260 3,398 6,78E-04

PMPCA mitochondrial-processing peptidase subunit alpha precursor 2,79E-04 4,60E-05 6,075 3,398 6,78E-04 npepps puromycin-sensitive aminopeptidase 3,00E-05 1,85E-04 6,151 -3,355 7,95E-04 zpbp1 zona pellucida-binding protein 1 isoform 2 0,004449361 0,006304115 1,417 -3,328 8,75E-04 LDHAL6B L-lactate dehydrogenase A-like 6B 0,006196426 0,005257715 1,179 3,327 8,78E-04 Gm2199 mitochondrial carrier homolog 2 0,00232098 0,001167426 1,988 3,322 8,95E-04 Stoml2 stomatin-like protein 2, mitochondrial 9,08E-05 4,67E-04 5,143 -3,307 9,42E-04 ATP5B ATP synthase subunit beta, mitochondrial precursor 0,00822562 0,009936516 1,208 -3,307 9,45E-04 N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase isoform 1 aga precursor 6,08E-05 4,81E-04 7,911 -3,302 9,59E-04 ACRBP acrosin-binding protein isoform 1 precursor 0,001834115 0,003030314 1,652 -3,293 9,92E-04

Zp3r zona pellucida sperm-binding protein 3 receptor precursor 0,001208789 0,002057406 1,702 -3,259 0,001117218 lrrc23 leucine-rich repeat-containing protein 23 7,27E-05 4,42E-04 6,078 -3,238 0,001205791 #N/A insulin-degrading enzyme 2,66E-05 1,59E-04 6,000 -3,238 0,001205791 SPATA20 spermatogenesis-associated protein 20 2,67E-04 8,34E-05 3,201 3,221 0,001279004 tpp2 tripeptidyl-peptidase 2 5,27E-04 3,10E-04 1,700 3,196 0,001395346 Serpina1 PREDICTED: alpha-1-antiproteinase-like 0,001465453 7,67E-04 1,909 3,191 0,001415883 Hba-a1 hemoglobin alpha, adult chain 2 0,002400706 0,001116662 2,150 3,175 0,001498245 NIPSNAP3A nipsnap homolog 3A 0,003642805 0,001875618 1,942 3,137 0,001707106 LOC677611 casein kinase II subunit alpha 3,21E-04 8,70E-04 2,713 -3,129 0,001752824 gpi1 glucose-6-phosphate isomerase 0,001850572 0,002862517 1,547 -3,126 0,001769162 4933400C05Rik spermatogenesis-associated protein 32 2,59E-04 8,15E-04 3,150 -3,122 0,001795811 iars2 isoleucine--tRNA ligase, mitochondrial precursor 7,97E-04 4,81E-04 1,656 3,120 0,001809436 VPS13A vacuolar protein sorting-associated protein 13A 6,29E-05 1,48E-04 2,354 -3,117 0,00182488 UGP2 UTP--glucose-1-phosphate uridylyltransferase 4,64E-05 2,59E-04 5,583 -3,117 0,001827212 XPO7 exportin-7 1,47E-04 3,13E-05 4,688 3,108 0,001881314 Ropn1 ropporin-1 0,003890162 0,005343302 1,374 -3,103 0,00191442 EF-hand calcium-binding domain-containing protein 6 EFCAB6 isoform 1 3,53E-04 5,64E-04 1,599 -3,099 0,001943328 Fam71a uncharacterized protein LOC619288 5,77E-04 0,001124802 1,948 -3,082 0,002058777 2810408A11Rik protein phosphatase inhibitor 2-like 3,25E-04 9,38E-04 2,889 -3,053 0,002269395 cAMP-dependent protein kinase catalytic subunit alpha Prkaca isoform 2 0,001111348 0,001867731 1,681 -3,049 0,002297351 NUBPL iron-sulfur protein NUBPL precursor 1,45E-04 5,90E-04 4,065 -3,046 0,002319233 RTDR1 rhabdoid tumor deletion region gene 1 isoform 1 6,62E-04 2,47E-04 2,680 3,036 0,002396572 CCDC38 coiled-coil domain-containing protein 38 1,62E-04 2,20E-05 7,366 3,030 0,002445299 psmd7 26S proteasome non-ATPase regulatory subunit 7 1,64E-04 6,07E-04 3,703 -3,028 0,002459612 Ttc39d tetratricopeptide repeat domain 39D 1,31E-04 4,26E-04 3,254 -3,015 0,002570434 5730469M10Rik redox-regulatory protein FAM213A 6,41E-04 1,98E-04 3,232 2,993 0,002761038 NME4 nucleoside diphosphate kinase, mitochondrial precursor 0,00117722 3,57E-04 3,298 2,993 0,002761038 Dnahc1 dynein, axonemal, heavy chain 1 6,36E-05 1,24E-04 1,945 -2,963 0,003044952 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial OXSM precursor 7,90E-04 3,61E-04 2,188 2,960 0,003077155 methylmalonate-semialdehyde dehydrogenase [acylating], Aldh6a1 mitochondrial precursor 3,67E-04 7,57E-04 2,061 -2,935 0,003335928 acsbg2 long-chain-fatty-acid--CoA ligase ACSBG2 6,14E-04 0,001063661 1,734 -2,922 0,003472342 xpo1 exportin-1 2,11E-05 1,38E-04 6,575 -2,922 0,003477438 Dnhc12 PREDICTED: dynein heavy chain 12, axonemal 5,66E-05 1,11E-04 1,954 -2,908 0,003642214 Acox3 peroxisomal acyl-coenzyme A oxidase 3 9,63E-05 2,97E-04 3,081 -2,905 0,003667297 LOC641192 heat shock cognate 71 kDa protein 6,70E-04 3,52E-04 1,904 2,896 0,003782106

SPACA1 sperm acrosome membrane-associated protein 1 precursor 0,015771523 0,011496927 1,372 2,891 0,003845365

ACAD9 acyl-CoA dehydrogenase family member 9, mitochondrial 4,10E-04 7,42E-04 1,807 -2,877 0,004018632 HYDIN hydrocephalus-inducing protein 3,75E-05 8,17E-05 2,177 -2,868 0,004128968 Prdx5 peroxiredoxin-5, mitochondrial precursor 0,001178548 0,002102354 1,784 -2,862 0,004211677 ARMC3 armadillo repeat-containing protein 3 isoform 1 1,89E-04 3,88E-04 2,050 -2,855 0,004301996 SPATC1 speriolin 0,001991203 0,001259147 1,581 2,841 0,004497059 MUC5B mucin 5, subtype B, tracheobronchial precursor 3,54E-05 5,70E-06 6,213 2,840 0,004508398 LMNB1 lamin-B1 1,25E-04 1,89E-05 6,605 2,840 0,004508398 Gm6685 cytochrome b5 domain-containing protein 1 6,20E-04 1,78E-04 3,493 2,834 0,004590329 CCDC105 coiled-coil domain-containing protein 105 0,001150891 0,001754358 1,524 -2,830 0,004652939 fsip2 PREDICTED: fibrous sheath-interacting protein 2 1,53E-04 2,28E-04 1,484 -2,830 0,004652939 mitochondrial import inner membrane translocase subunit Timm44 TIM44 6,09E-04 0,00103557 1,700 -2,768 0,005641499 Gm1096 PREDICTED: CD177 antigen isoform 2 0,001153694 5,11E-04 2,258 2,733 0,006271458 high affinity cAMP-specific and IBMX-insensitive 3,5-cyclic PDE8A phosphodiesterase 8A 3,51E-05 1,67E-04 4,767 -2,731 0,006318384 chdh choline dehydrogenase, mitochondrial 7,24E-04 4,34E-04 1,668 2,730 0,006336274 KRT10 keratin, type I cytoskeletal 10 0,001137871 6,04E-04 1,885 2,695 0,007044662 hsd17b4 peroxisomal multifunctional enzyme type 2 5,92E-05 2,12E-04 3,590 -2,680 0,007355273 4921507P07Rik uncharacterized protein C7orf31 homolog 1,51E-04 4,52E-04 2,989 -2,679 0,007385035 cation channel sperm-associated protein subunit beta CATSPERB precursor 4,07E-04 2,17E-04 1,875 2,679 0,007387459 st13 hsc70-interacting protein 1,56E-04 5,44E-04 3,491 -2,673 0,007518697 serine/threonine-protein phosphatase PP1-gamma catalytic LOC627816 subunit 8,06E-04 3,72E-04 2,170 2,670 0,007578263 Tex101 testis-expressed sequence 101 protein precursor 0,002544181 0,001396415 1,822 2,663 0,007747853 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia

CECR5 cat eye syndrome critical region protein 5 homolog precursor 8,18E-04 0,00142685 1,745 -2,648 0,008105242 camk4 calcium/calmodulin-dependent protein kinase type IV 4,35E-05 2,60E-04 5,970 -2,643 0,008210054 CS citrate synthase, mitochondrial precursor 0,004193326 0,005511836 1,314 -2,600 0,009310536 HK1 hexokinase-1 isoform HK1-sb 0,004577325 0,005196722 1,135 -2,600 0,009322018 isochorismatase domain-containing protein 2A, Isoc2a mitochondrial precursor 0,003428162 0,001910477 1,794 2,581 0,009851017 AKAP3 A-kinase anchor protein 3 0,003164035 0,002906583 1,089 2,575 0,010036055 DPP3 dipeptidyl peptidase 3 4,68E-05 1,81E-04 3,865 -2,564 0,010338024 Glipr1l3 GLI pathogenesis-related 1 like 1 precursor 0,001749175 9,98E-04 1,753 2,554 0,010658688 mmel1 membrane metallo-endopeptidase-like 1 7,61E-05 2,40E-04 3,150 -2,548 0,010837183 Fndc8 fibronectin type III domain-containing protein 8 8,98E-04 0,001672348 1,862 -2,546 0,010883406 ak1 adenylate kinase isoenzyme 1 isoform 1 0,00200719 0,002974642 1,482 -2,517 0,011836055

COX2 cytochrome c oxidase subunit II [Mus musculus castaneus] 0,00974733 0,007415808 1,314 2,515 0,011908499 Ccdc19 nasopharyngeal epithelium specific protein 1 3,62E-04 6,64E-04 1,832 -2,512 0,012011466 Fam162a protein FAM162A 0,001396192 5,87E-04 2,379 2,499 0,012452516 DKKL1 dickkopf-like protein 1 precursor 6,88E-05 3,49E-04 5,068 -2,495 0,012612459 Dnahc2 dynein heavy chain 2, axonemal 3,72E-05 7,41E-05 1,993 -2,493 0,012649677 BC051142 testis specific basic protein isoform 2 6,34E-04 1,50E-04 4,218 2,493 0,012660064 WDR65 WD repeat-containing protein 65 4,81E-04 7,05E-04 1,464 -2,479 0,013162317 SSSCA1 Sjoegren syndrome/scleroderma autoantigen 1 homolog 1,63E-04 6,94E-04 4,251 -2,449 0,014341803 calcium-binding tyrosine phosphorylation-regulated protein Cabyr isoform a 9,39E-04 4,26E-04 2,205 2,430 0,015083124 Psmd11 26S proteasome non-ATPase regulatory subunit 11 2,80E-04 5,73E-04 2,045 -2,430 0,0151079 psmd3 26S proteasome non-ATPase regulatory subunit 3 9,75E-05 2,67E-04 2,737 -2,428 0,015191464

PMPCB mitochondrial-processing peptidase subunit beta precursor 1,53E-04 3,00E-05 5,109 2,426 0,015256193 4930404N11Rik uncharacterized protein C19orf71 homolog 0,001890413 0,001126056 1,679 2,419 0,015546191 PSMD1 26S proteasome non-ATPase regulatory subunit 1 2,61E-04 4,69E-04 1,796 -2,405 0,016166802 Atp5a1 ATP synthase subunit alpha, mitochondrial precursor 0,006271242 0,007283678 1,161 -2,404 0,016236166 Dnahc6 axonemal dynein heavy chain 9,39E-05 1,47E-04 1,571 -2,400 0,016384946 pfkp 6-phosphofructokinase type C 0,001375013 0,001817018 1,321 -2,396 0,016570462 CCDC146 coiled-coil domain containing 146 9,80E-05 2,68E-05 3,652 2,378 0,017387703

PGRMC1 membrane-associated progesterone receptor component 1 4,52E-04 1,36E-04 3,321 2,378 0,017387703 Gm8566 superoxide dismutase [Cu-Zn] 0,001518428 7,71E-04 1,971 2,358 0,018353545 Ldhc L-lactate dehydrogenase C chain 0,010370016 0,011579181 1,117 -2,351 0,018713725 ropn1l ropporin-1-like protein 8,28E-04 3,88E-04 2,135 2,347 0,018947872 GPX5 epididymal secretory glutathione peroxidase precursor 7,28E-04 3,00E-04 2,423 2,340 0,019278844 1700019O17Rik uncharacterized protein C2orf57 homolog 7,09E-04 3,13E-04 2,261 2,340 0,019278844 TAGLN transgelin 3,06E-04 7,58E-04 2,481 -2,339 0,019314647 lipe hormone-sensitive lipase isoform 1 5,38E-04 3,25E-04 1,654 2,329 0,0198597 iron-sulfur cluster co-chaperone protein HscB, mitochondrial hscB precursor 2,21E-04 5,71E-04 2,578 -2,318 0,020447584 ACTL9 actin-like protein 9 9,35E-05 3,64E-04 3,897 -2,298 0,021539623 1700093K21Rik uncharacterized protein C2orf74 homolog 1,65E-04 6,04E-04 3,657 -2,298 0,021539623 Car2 carbonic anhydrase 2 0,001393316 8,87E-04 1,572 2,287 0,022200036 Gm7308 calmodulin 0,001805506 9,30E-04 1,942 2,287 0,022200036 PPEF1 serine/threonine-protein phosphatase with EF-hands 1 9,08E-05 2,56E-04 2,816 -2,286 0,022233174 - UPF0704 protein C6orf165 homolog 1,08E-04 3,05E-04 2,815 -2,286 0,022233174

1110017D15Rik spermatid-specific manchette-related protein 1 isoform 1 0,001730347 0,001230741 1,406 2,282 0,022513419 psmc4 26S protease regulatory subunit 6B 1,13E-04 3,52E-04 3,125 -2,279 0,022651652 glb1l beta-galactosidase-1-like protein precursor 7,09E-05 2,25E-04 3,173 -2,279 0,022651652 Prdx6-rs1 peroxiredoxin 6, related sequence 1 1,33E-04 4,33E-04 3,255 -2,279 0,022651652

4932412H11Rik leucine-rich repeat and death domain-containing protein 1 1,30E-04 3,00E-04 2,307 -2,274 0,022959775 Gm5506 alpha-enolase 0,005055182 0,004177317 1,210 2,262 0,023684847 Wdr96 WD repeat domain 96 9,96E-05 1,97E-04 1,977 -2,261 0,023733865 solute carrier family 2, facilitated glucose transporter SLC2A5 member 5 0,002157979 0,001433678 1,505 2,257 0,024021125 Ubxn11 UBX domain-containing protein 11 5,23E-04 2,66E-04 1,963 2,246 0,024736031 1700018L24Rik testis-expressed sequence 29 protein 0,00116219 6,18E-04 1,880 2,229 0,025833327 SPAG6 sperm-associated antigen 6 0,001245622 0,001875728 1,506 -2,222 0,0262721 Adam6b a disintegrin and metalloproteinase domain 6-like 3,67E-04 6,53E-04 1,781 -2,222 0,026287424 FSCN3 fascin-3 4,13E-04 1,86E-04 2,220 2,220 0,026420433 Hsp90b1 endoplasmin precursor 3,10E-04 1,64E-04 1,893 2,207 0,027281438 CSNK1G2 casein kinase I isoform gamma-2 isoform 1 1,75E-04 4,30E-04 2,459 -2,191 0,028429048 NME2 nucleoside diphosphate kinase B 1,10E-04 5,06E-04 4,602 -2,174 0,029727956 LOC100047329 40S ribosomal protein S18 4,23E-04 1,39E-04 3,044 2,173 0,029764511 SUOX sulfite oxidase, mitochondrial precursor 2,26E-04 7,16E-05 3,162 2,173 0,029764511 - PREDICTED: uncharacterized protein LOC77908 0,00324911 0,001835138 1,770 2,162 0,030649881 cpvl probable serine carboxypeptidase CPVL precursor 2,50E-04 5,55E-04 2,223 -2,157 0,031003895 lonp1 lon protease homolog, mitochondrial precursor 3,38E-04 1,89E-04 1,787 2,149 0,031655876 1700016C15Rik uncharacterized protein C1orf100 homolog 0,001110394 4,33E-04 2,567 2,147 0,031824152 4931432M23Rik inactive serine protease 54 precursor 9,55E-05 3,66E-04 3,838 -2,141 0,032263085 IL4I1 L-amino-acid oxidase precursor 0,001514632 0,001965055 1,297 -2,140 0,032345008 tomm40 mitochondrial import receptor subunit TOM40 homolog 1,68E-04 5,04E-04 2,996 -2,136 0,03267557 Akr1b3 aldose reductase 1,47E-04 4,20E-04 2,865 -2,136 0,03267557 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA10 subunit 10, mitochondrial precursor 0,001176298 0,001794734 1,526 -2,133 0,032907353 PGD 6-phosphogluconate dehydrogenase, decarboxylating 7,39E-05 2,40E-04 3,252 -2,132 0,033033961 GOT1 aspartate aminotransferase, cytoplasmic 6,63E-05 2,13E-04 3,216 -2,132 0,033033961 Gm16399 cytochrome c oxidase subunit 6C 0,005556151 0,004448522 1,249 2,128 0,033357596 Lcn5 epididymal-specific lipocalin-5 isoform a precursor 4,46E-04 9,67E-04 2,166 -2,124 0,033680253 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia CCDC42 coiled-coil domain-containing protein 42A 2,35E-04 5,10E-04 2,167 -2,124 0,033680253 PLBD2 putative phospholipase B-like 2 precursor 2,00E-04 4,48E-04 2,239 -2,124 0,033680253

- PREDICTED: uncharacterized protein C17orf47 homolog 0,001002824 7,06E-04 1,420 2,123 0,033738029 TEKT2 tektin-2 0,001748578 0,001364028 1,282 2,122 0,033813137 4930579J09Rik protein phosphatase 1 regulatory subunit 32 2,92E-04 5,99E-04 2,052 -2,119 0,034056282 voltage-dependent anion-selective channel protein 3 isoform Gm7591 2 0,001540303 0,001063872 1,448 2,118 0,034172921 alb serum albumin precursor 7,37E-04 5,34E-04 1,381 2,115 0,034462661 PREDICTED: NADH dehydrogenase [ubiquinone] 1 beta Gm3244 subcomplex subunit 4-like 0,001594838 7,33E-04 2,176 2,105 0,035321602 nagK N-acetyl-D-glucosamine kinase isoform 1 5,50E-04 9,80E-04 1,781 -2,096 0,036064823 Gm13050 elongation factor 2 1,07E-04 3,72E-05 2,870 2,086 0,036957679 LOC100048867 annexin A2 2,24E-04 8,23E-05 2,718 2,086 0,036957679 KRT2 keratin, type II cytoskeletal 2 epidermal 5,62E-04 3,30E-04 1,700 2,074 0,038052584 Gm6242 myosin regulatory light chain 12B 3,83E-04 1,24E-04 3,083 2,060 0,0394255 1190002A17Rik adenylate kinase 8 6,97E-04 0,001003532 1,441 -2,055 0,039908786 hspb9 heat shock protein beta-9 0,002248648 0,001599544 1,406 2,022 0,043158255 1700065I17Rik uncharacterized protein C5orf48 homolog 0,001338791 5,59E-04 2,397 2,008 0,044681778

UCHL5 ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 1 1,71E-04 4,20E-04 2,450 -2,007 0,044707724 Fam82b regulator of microtubule dynamics protein 1 1,69E-04 4,17E-04 2,465 -2,007 0,044707724 odf3 outer dense fiber protein 3 0,002903542 0,004100466 1,412 -2,005 0,044959856 serine/threonine-protein phosphatase PGAM5, mitochondrial pgam5 isoform 2 7,75E-04 0,00118842 1,533 -2,003 0,045158808 L-2-hydroxyglutarate dehydrogenase, mitochondrial L2HGDH precursor 0,001121286 0,00153357 1,368 -1,995 0,0460901 Adgb androglobin 1,79E-05 6,04E-05 3,370 -1,976 0,048167134 - uncharacterized protein LOC69439 4,53E-05 1,24E-04 2,731 -1,975 0,048253389 Hyal5 hyaluronidase PH-20 4,88E-04 8,37E-04 1,716 -1,969 0,048911699 phosphatidylethanolamine-binding protein 4 isoform a Pebp4 precursor 0,001105914 0,001779406 1,609 -1,959 0,050158713 EFHB EF-hand domain-containing family member B 1,24E-04 2,49E-04 2,014 -1,956 0,050410506 lmna prelamin-A/C isoform C 3,18E-04 1,80E-04 1,769 1,952 0,050883858 cpt2 carnitine O-palmitoyltransferase 2, mitochondrial precursor 6,61E-04 9,52E-04 1,440 -1,932 0,05334918 HSPA5 78 kDa glucose-regulated protein precursor 3,68E-04 5,56E-04 1,513 -1,917 0,055204109

Fahd2a fumarylacetoacetate hydrolase domain-containing protein 2A 0,001935646 0,002582043 1,334 -1,916 0,055420503 EFCAB5 EF-hand calcium-binding domain-containing protein 5 6,97E-05 1,34E-04 1,928 -1,915 0,055535282 Fam71e2 uncharacterized protein LOC243822 1,62E-04 3,24E-04 2,000 -1,915 0,055535282 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA9 subunit 9, mitochondrial precursor 8,51E-04 0,001325477 1,558 -1,904 0,056872176 spermatogenesis-associated protein 19, mitochondrial SPATA19 precursor 0,001223858 6,14E-04 1,994 1,894 0,058239111 dld dihydrolipoyl dehydrogenase, mitochondrial precursor 0,004114163 0,003469746 1,186 1,893 0,058396436 Pgcp carboxypeptidase Q precursor 2,46E-04 9,55E-05 2,574 1,876 0,060623148 DNAJB8 dnaJ homolog subfamily B member 8 4,21E-04 1,69E-04 2,482 1,876 0,060623148 4930544G11Rik ras homolog gene family, member A-like 2,69E-04 7,17E-04 2,662 -1,860 0,062828263 Gk2 glycerol kinase 2 0,001312167 0,001827615 1,393 -1,850 0,064249023 Prss46 serine protease 46 0,002958748 0,00210585 1,405 1,849 0,064497462 LOC675857 transitional endoplasmic reticulum ATPase 0,001181458 0,001460363 1,236 -1,848 0,064589507 CASC1 cancer susceptibility candidate protein 1 1,47E-04 2,73E-04 1,864 -1,835 0,066455536 Gm13570 brain protein 44-like protein 2 0,003514886 0,002277964 1,543 1,835 0,066525997 Atp5d ATP synthase subunit delta, mitochondrial precursor 0,001250396 6,40E-04 1,954 1,833 0,066745701 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex Ndufa7 subunit 7 4,32E-04 8,97E-04 2,078 -1,831 0,067172697 LOC100047986 elongation factor 1-gamma 5,65E-04 3,36E-04 1,683 1,825 0,068053763 PREDICTED: glyceraldehyde-3-phosphate dehydrogenase- Gm4335 like isoform 2 0,001520003 0,002040427 1,342 -1,823 0,068239904 SLC30A9 zinc transporter 9 1,27E-04 4,15E-05 3,061 1,821 0,068665628 abhd11 alpha/beta hydrolase domain-containing protein 11 isoform 1 3,42E-04 1,14E-04 3,010 1,821 0,068665628 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase PLCZ1 zeta-1 1,47E-04 4,64E-05 3,173 1,821 0,068665628 PMM2 phosphomannomutase 2 1,98E-04 6,07E-05 3,254 1,821 0,068665628 Gm6204 40S ribosomal protein S14 4,23E-04 1,40E-04 3,032 1,821 0,068665628 EFHC1 EF-hand domain-containing protein 1 8,32E-04 0,001108667 1,332 -1,819 0,068953296 Qsox1 sulfhydryl oxidase 1 isoform b precursor 2,87E-05 1,15E-04 3,991 -1,813 0,069809743 LOC630729 glutathione reductase, mitochondrial precursor 8,14E-05 2,33E-04 2,865 -1,811 0,070108422 Oit1 protein FAM3D precursor 1,02E-04 3,21E-04 3,166 -1,802 0,071621785 pyruvate dehydrogenase phosphatase regulatory subunit, 4930402E16Rik mitochondrial precursor 2,16E-04 1,14E-04 1,899 1,777 0,075492704 1700019N12Rik testis-expressed sequence 40 protein 9,84E-04 5,47E-04 1,799 1,777 0,075492704 psmA2 proteasome subunit alpha type-2 5,99E-04 0,001033864 1,726 -1,761 0,078211558 NADH dehydrogenase [ubiquinone] 1 beta subcomplex NDUFB9 subunit 9 0,001708703 0,001179033 1,449 1,754 0,079494303 - regulator of G-protein signaling 22 5,39E-05 1,13E-04 2,088 -1,747 0,080553785 disintegrin and metalloproteinase domain-containing protein Adam5 5 isoform 1 precursor 1,06E-04 2,01E-04 1,899 -1,742 0,081505875 Cpt1b carnitine O-palmitoyltransferase 1, muscle isoform 3,92E-04 5,94E-04 1,515 -1,735 0,082679188 HTRA2 serine protease HTRA2, mitochondrial 5,88E-04 9,54E-04 1,623 -1,735 0,082679188 NADH dehydrogenase [ubiquinone] 1 beta subcomplex Ndufb7 subunit 7 0,001873144 0,001313313 1,426 1,728 0,083996349 Gm12141 60 kDa heat shock protein, mitochondrial 0,003417246 0,003763253 1,101 -1,726 0,084288609 Gm7795 cytochrome c oxidase subunit 6A1, mitochondrial 0,001494591 5,76E-04 2,597 1,726 0,084345998 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia ARMC4 armadillo repeat-containing protein 4 4,80E-04 6,47E-04 1,348 -1,726 0,084347931 trim69 E3 ubiquitin-protein ligase TRIM69 3,03E-04 5,00E-04 1,648 -1,724 0,084625036 Gm5347 a disintegrin and metallopeptidase domain 34 2,14E-04 4,12E-04 1,931 -1,711 0,087012169 REEP6 receptor expression-enhancing protein 6 isoform 1 6,05E-04 0,001100395 1,819 -1,694 0,090215387 Wdr78 WD repeat-containing protein 78 9,89E-05 1,92E-04 1,942 -1,694 0,090215387 complement decay-accelerating factor, GPI-anchored Cd55 precursor 4,34E-04 7,86E-04 1,812 -1,692 0,090734417 Crat carnitine O-acetyltransferase 0,003778255 0,004584597 1,213 -1,687 0,091628035 FABP4 fatty acid-binding protein, adipocyte 2,44E-04 6,68E-05 3,650 1,682 0,092623335 C030006K11Rik UPF0598 protein C8orf82 homolog isoform 1 2,11E-04 6,28E-05 3,354 1,682 0,092623335 LOC633860 3(2),5-bisphosphate nucleotidase 1 1,41E-04 4,14E-05 3,397 1,682 0,092623335 csnk2a2 casein kinase II subunit alpha 0,001812506 0,00233044 1,286 -1,675 0,093860931 tekt1 tektin-1 0,001027897 0,001421598 1,383 -1,674 0,094150752 Tuba3a tubulin alpha-3 chain 0,001872541 0,002186908 1,168 -1,673 0,094274796 CCDC113 coiled-coil domain-containing protein 113 9,72E-05 2,46E-04 2,531 -1,671 0,0947534 ACTL7A actin-like protein 7A 9,45E-04 0,00130456 1,380 -1,666 0,095677666 translocon-associated protein subunit delta isoform 1 Ssr4 precursor 6,89E-04 2,93E-04 2,351 1,655 0,098011258 TCTE1 T-complex-associated testis-expressed protein 1 8,71E-05 2,40E-04 2,751 -1,639 0,101220632 PREDICTED: orphan sodium- and chloride-dependent Ntt5 neurotransmitter transporter NTT5 1,15E-04 2,92E-04 2,552 -1,639 0,101220632 1700026L06Rik uncharacterized protein C9orf9 homolog 0,001692537 0,001100807 1,538 1,639 0,101233118 vamp3 vesicle-associated membrane protein 3 0,003036904 0,002123402 1,430 1,629 0,10323561 rps3 40S ribosomal protein S3 3,71E-04 1,97E-04 1,881 1,628 0,103522906 glud1 glutamate dehydrogenase 1, mitochondrial precursor 2,25E-04 3,65E-04 1,626 -1,619 0,105390406 A330021E22Rik uncharacterized protein C7orf63 homolog 3,11E-05 8,28E-05 2,660 -1,617 0,105965592 ACAA2 3-ketoacyl-CoA thiolase, mitochondrial 6,50E-05 1,97E-04 3,039 -1,617 0,105965592

Dhrs4 dehydrogenase/reductase SDR family member 4 isoform 1 0,001236229 8,67E-04 1,426 1,616 0,105998392 LOC100044663 apolipoprotein O isoform 1 0,002018784 0,001398155 1,444 1,614 0,106607805 Xpnpep1 xaa-Pro aminopeptidase 1 2,39E-05 8,14E-05 3,410 -1,613 0,106686357 Lrrc52 leucine-rich repeat-containing protein 52 precursor 9,17E-05 3,18E-04 3,473 -1,613 0,106686357 1700023E05Rik testicular haploid expressed gene protein-like 1,59E-04 2,94E-04 1,847 -1,608 0,107879512 IMMP2L mitochondrial inner membrane protease subunit 2 7,35E-04 4,22E-04 1,742 1,607 0,108147568 LOC100045796 cysteine desulfurase, mitochondrial 4,91E-04 6,96E-04 1,418 -1,604 0,108805689

HADHA trifunctional enzyme subunit alpha, mitochondrial precursor 0,001883598 0,002128039 1,130 -1,603 0,108846079 TTC29 tetratricopeptide repeat protein 29 1,86E-04 8,25E-05 2,259 1,599 0,109809233 Gm5562 glutathione S-transferase Mu 1 3,09E-04 1,48E-04 2,086 1,599 0,109809233

Abca17 ATP-binding cassette, sub-family A (ABC1), member 17 5,36E-05 1,08E-04 2,013 -1,597 0,110186268 Gm2903 10 kDa heat shock protein, mitochondrial 2,66E-04 5,62E-04 2,113 -1,597 0,110186268 pyruvate dehydrogenase E1 component subunit beta, Gm6123 mitochondrial precursor 0,008956886 0,007517306 1,192 1,597 0,110294703 LOC100045163 phospholipase B-like 1 precursor 5,38E-04 7,88E-04 1,465 -1,593 0,111164438 TTC25 tetratricopeptide repeat protein 25 4,32E-04 6,40E-04 1,479 -1,591 0,111690663 Prdx6 peroxiredoxin-6 3,24E-04 5,83E-04 1,803 -1,584 0,113101786 Acr acrosin isoform 3 0,002649188 0,003268046 1,234 -1,571 0,116202944 Cyct cytochrome c, testis-specific 8,20E-04 0,001382033 1,686 -1,571 0,116225133 cct5 T-complex protein 1 subunit epsilon 3,02E-04 4,54E-04 1,502 -1,564 0,117823971

AGPAT2 1-acyl-sn-glycerol-3-phosphate acyltransferase beta precursor 2,40E-04 8,18E-05 2,937 1,563 0,118135769 rps9 40S ribosomal protein S9 2,35E-04 8,30E-05 2,829 1,563 0,118135769

Slco6b1 solute carrier organic anion transporter family, member 6b1 1,43E-04 5,09E-05 2,816 1,563 0,118135769 Atp5c1 ATP synthase subunit gamma, mitochondrial isoform a 0,001863501 0,002450581 1,315 -1,562 0,118188637 CCDC40 coiled-coil domain-containing protein 40 5,05E-04 3,78E-04 1,337 1,556 0,119758499 pgls 6-phosphogluconolactonase 4,32E-04 2,00E-04 2,157 1,555 0,119951637 NADH dehydrogenase [ubiquinone] 1 beta subcomplex Gm9050 subunit 11, mitochondrial-like 8,63E-04 4,10E-04 2,106 1,555 0,119951637 H2-Ke6 estradiol 17-beta-dehydrogenase 8 0,001991804 0,002737824 1,375 -1,552 0,120655828 CSNK2B casein kinase II subunit beta 0,00130153 7,91E-04 1,646 1,529 0,126250747 Tmem126a transmembrane protein 126A 8,36E-04 4,26E-04 1,962 1,519 0,12865343 APOE apolipoprotein E precursor 3,17E-04 1,63E-04 1,948 1,519 0,12865343 - PREDICTED: uncharacterized protein LOC100862214 3,97E-04 2,34E-04 1,695 1,519 0,12865343 Izumo1 izumo sperm-egg fusion protein 1 precursor 0,003437175 0,004170218 1,213 -1,519 0,128762622 Gm15785 dnaJ homolog subfamily B member 6 isoform a 0,001233989 0,001012886 1,218 1,515 0,129672258 LOC100039128 nuclear pore complex protein Nup93 1,19E-04 2,05E-04 1,724 -1,514 0,130113496 DNAJB13 dnaJ homolog subfamily B member 13 6,97E-04 9,56E-04 1,372 -1,512 0,130511483 oplaH 5-oxoprolinase 2,44E-04 1,56E-04 1,564 1,510 0,130940643 wdr16 WD repeat-containing protein 16 8,44E-04 0,001084457 1,285 -1,508 0,131668819 SPAG17 sperm-associated antigen 17 8,19E-05 1,30E-04 1,584 -1,505 0,132221013 PARK7 protein DJ-1 6,28E-04 9,94E-04 1,584 -1,505 0,132221013 Odf3b outer dense fiber protein 3B 9,23E-04 7,01E-04 1,318 1,502 0,132978982 Arf6 ADP-ribosylation factor 6 2,49E-04 5,13E-04 2,065 -1,502 0,133181388 KRT1 keratin, type II cytoskeletal 1 9,27E-04 6,83E-04 1,357 1,501 0,133372362 Ccdc136 coiled-coil domain-containing protein 136 isoform 2 8,30E-05 1,60E-04 1,928 -1,498 0,13401638 vapa vesicle-associated membrane protein-associated protein A 3,22E-04 6,27E-04 1,945 -1,498 0,13401638 Gm9047 uncharacterized protein LOC668210 0,00118929 8,33E-04 1,429 1,497 0,134328414 PRSS1 protease, serine, 1 precursor 0,002423699 0,001755553 1,381 1,495 0,134942489 CCDC11 coiled-coil domain-containing protein 11 4,35E-04 3,08E-04 1,411 1,494 0,135110169 1700001P01Rik uncharacterized protein C17orf98 homolog 7,70E-04 4,26E-04 1,807 1,490 0,136110986 Gm9093 60S acidic ribosomal protein P0 3,92E-04 2,19E-04 1,788 1,490 0,136110986 NIPSNAP3B protein NipSnap homolog 3B 2,98E-04 1,31E-04 2,285 1,480 0,138907325 CYB5 cytochrome b5 5,77E-04 2,43E-04 2,377 1,480 0,138907325 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia BLVRB flavin reductase (NADPH) 3,28E-04 1,42E-04 2,307 1,480 0,138907325 GNPAT dihydroxyacetone phosphate acyltransferase 4,85E-05 1,14E-04 2,345 -1,457 0,145046476 pdcd6 programmed cell death protein 6 1,93E-04 4,39E-04 2,277 -1,457 0,145046476 GALC galactocerebrosidase precursor 8,83E-05 1,98E-04 2,238 -1,457 0,145046476 EFR3A protein EFR3 homolog A 4,21E-05 1,01E-04 2,392 -1,457 0,145046476 Gm5678 carbonyl reductase [NADPH] 1 2,89E-04 5,18E-04 1,791 -1,454 0,145876925 arsA arylsulfatase A precursor 4,23E-04 7,11E-04 1,681 -1,454 0,145876925 PREDICTED: ES1 protein homolog, mitochondrial-like LOC100046684 isoform 1 4,03E-04 6,91E-04 1,714 -1,447 0,147780614 LOC100048880 AFG3-like protein 2 1,47E-04 2,34E-04 1,586 -1,446 0,148043358

COX7A2 cytochrome c oxidase subunit 7A2, mitochondrial precursor 7,14E-04 0,00145053 2,033 -1,442 0,149429242 SQRDL sulfide:quinone oxidoreductase, mitochondrial 6,33E-04 8,45E-04 1,336 -1,441 0,149687069 PGAM2 phosphoglycerate mutase 2 0,004041497 0,00470782 1,165 -1,428 0,153324397 histidine triad nucleotide-binding protein 2, mitochondrial HINT2 precursor 3,98E-04 1,93E-04 2,068 1,420 0,155749002 PREDICTED: sterile alpha motif domain-containing protein Samd15 15 1,24E-04 5,78E-05 2,153 1,420 0,155749002 4930412F15Rik protein FAM221B 6,28E-05 1,56E-04 2,478 -1,419 0,155795105 cyb5r2 NADH-cytochrome b5 reductase 2 isoform 2 1,06E-04 2,55E-04 2,403 -1,419 0,155795105 Wfdc15b WAP four-disulfide core domain protein 15B precursor 2,60E-04 6,85E-04 2,630 -1,419 0,155795105 Got2 aspartate aminotransferase, mitochondrial 9,96E-04 7,36E-04 1,353 1,417 0,156577902 maestro heat-like repeat-containing protein family member Heatr7b2 2B 3,91E-04 4,86E-04 1,243 -1,415 0,157081082 Gm10108 cytochrome c, somatic 5,17E-04 9,02E-04 1,743 -1,409 0,158829366 SLC44A5 choline transporter-like protein 5 4,84E-04 6,72E-04 1,390 -1,403 0,160495986 CUB and zona pellucida-like domain-containing protein 1 CUZD1 precursor 9,61E-04 7,29E-04 1,317 1,397 0,162314938 TEKT5 tektin-5 0,001667094 0,001379043 1,209 1,397 0,162411423 SCP2 non-specific lipid-transfer protein 3,55E-05 1,10E-04 3,109 -1,397 0,162544471 CAR1 carbonic anhydrase 1 7,87E-04 5,34E-04 1,473 1,396 0,16278043 PSMD12 26S proteasome non-ATPase regulatory subunit 12 1,86E-04 2,99E-04 1,606 -1,392 0,163826213 TTC18 tetratricopeptide repeat domain 18 isoform 3 1,67E-04 2,65E-04 1,581 -1,389 0,164726514 serine/threonine-protein phosphatase 2B catalytic subunit PPP3CC gamma isoform 3,26E-04 5,20E-04 1,593 -1,389 0,164726514 DNAJC11 dnaJ homolog subfamily C member 11 5,74E-04 4,31E-04 1,333 1,384 0,166428485 cat catalase 7,62E-05 2,65E-05 2,880 1,383 0,166572314 RPS25 PREDICTED: 40S ribosomal protein S25-like 2,01E-04 8,13E-05 2,470 1,383 0,166572314 disintegrin and metalloproteinase domain-containing protein ADAM32 32 precursor 6,70E-05 2,29E-05 2,922 1,383 0,166572314 snx3 sorting nexin-3 2,07E-04 6,96E-05 2,970 1,383 0,166572314 Tssk2 testis-specific serine/threonine-protein kinase 2 1,23E-04 2,39E-04 1,946 -1,383 0,16657588 LOC100044248 uncharacterized protein C12orf60 homolog 1,83E-04 3,38E-04 1,851 -1,383 0,16657588 Tmem11 transmembrane protein 11, mitochondrial isoform 1 3,42E-04 6,71E-04 1,962 -1,383 0,16657588 PPT1 palmitoyl-protein thioesterase 1 precursor 2,20E-04 4,09E-04 1,857 -1,383 0,16657588 Canx calnexin precursor 4,96E-04 3,06E-04 1,620 1,382 0,167124483 COPS4 COP9 signalosome complex subunit 4 2,02E-04 9,97E-05 2,025 1,374 0,169422182 phosphatidylglycerophosphatase and protein-tyrosine ptpmt1 phosphatase 1 9,62E-04 7,08E-04 1,358 1,368 0,171187455 Gm4763 uncharacterized protein LOC210155 0,001832151 0,001270718 1,442 1,365 0,172198195 ANKRD5 ankyrin repeat and EF-hand domain-containing protein 1 2,60E-04 1,74E-04 1,493 1,365 0,172371277 Sod2 superoxide dismutase [Mn], mitochondrial precursor 0,001323928 0,001968067 1,487 -1,364 0,17256753 D-beta-hydroxybutyrate dehydrogenase, mitochondrial BDH1 precursor 0,001725233 0,001483235 1,163 1,360 0,173750535 NADH dehydrogenase [ubiquinone] flavoprotein 1, Ndufv1 mitochondrial precursor 0,001281086 0,001594127 1,244 -1,349 0,177390098 Ccdc116 coiled-coil domain-containing protein 116 1,88E-04 3,58E-04 1,898 -1,346 0,178390653 EFHC2 EF-hand domain-containing family member C2 8,24E-04 6,45E-04 1,276 1,340 0,180212784 ENKUR enkurin 9,85E-04 0,001354008 1,375 -1,330 0,18360689 LOC100044101 thioredoxin reductase 2, mitochondrial precursor 9,84E-05 1,96E-04 1,991 -1,324 0,185493268 KCNU1 potassium channel subfamily U member 1 6,12E-05 1,33E-04 2,176 -1,324 0,185493268 POLDIP2 polymerase delta-interacting protein 2 1,07E-04 2,21E-04 2,063 -1,324 0,185493268 Pdzk1 Na(+)/H(+) exchange regulatory cofactor NHE-RF3 6,31E-05 1,29E-04 2,043 -1,324 0,185493268

ACOT7 cytosolic acyl coenzyme A thioester hydrolase isoform 2 1,34E-04 2,72E-04 2,038 -1,324 0,185493268 Psmc6 26S protease regulatory subunit 10B 2,14E-04 3,61E-04 1,689 -1,321 0,186596218 etfA electron transfer flavoprotein subunit alpha, mitochondrial 2,58E-04 1,51E-04 1,708 1,312 0,189622241 RAB18 ras-related protein Rab-18 isoform 2 precursor 3,36E-04 1,99E-04 1,689 1,312 0,189622241 PREDICTED: phosphate carrier protein, mitochondrial-like SLC25A3 isoform 1 0,001129638 9,44E-04 1,197 1,301 0,193320215

Uqcrc2 cytochrome b-c1 complex subunit 2, mitochondrial precursor 0,006359046 0,007346425 1,155 -1,295 0,195306114 PSMD2 26S proteasome non-ATPase regulatory subunit 2 4,17E-04 5,59E-04 1,339 -1,286 0,198514843 NDUFAF2 mimitin, mitochondrial 1,92E-04 8,80E-05 2,184 1,282 0,199987303 LRP2BP LRP2-binding protein 1,53E-04 6,66E-05 2,305 1,282 0,199987303 LAP3 cytosol aminopeptidase 0,001376879 0,001714935 1,246 -1,275 0,202253426 SDR39U1 epimerase family protein SDR39U1 0,001030104 0,001403069 1,362 -1,268 0,20468992 cAMP-dependent protein kinase type I-alpha regulatory PRKAR1A subunit 9,14E-04 7,13E-04 1,282 1,267 0,205158139 Prss39 inactive serine protease 39 precursor 0,001312689 0,001018452 1,289 1,267 0,205158139

Gm13910 trifunctional enzyme subunit beta, mitochondrial precursor 0,002572318 0,003116362 1,211 -1,264 0,206055238 CLTC clathrin heavy chain 1 8,58E-05 5,20E-05 1,650 1,259 0,207944734 Defb22 beta-defensin 22 precursor 0,001934416 0,001538171 1,258 1,258 0,208355506 THEG testicular haploid expressed gene protein isoform 1 0,002067497 0,001899424 1,088 1,255 0,209536719 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia HTRA1 serine protease HTRA1 precursor 3,28E-04 2,12E-04 1,546 1,240 0,215043638 ACSL1 long-chain-fatty-acid--CoA ligase 1 0,002157522 0,002442966 1,132 -1,238 0,215881413 dolichyl-diphosphooligosaccharide--protein RPN1 glycosyltransferase subunit 1 precursor 2,32E-04 1,50E-04 1,544 1,233 0,217523593 Sept7 septin-7 isoform 1 4,09E-04 5,79E-04 1,417 -1,227 0,219665358 Nnt NAD(P) transhydrogenase, mitochondrial precursor 3,50E-04 2,47E-04 1,419 1,224 0,221007774 cation channel sperm-associated protein subunit gamma 2 Catsperg2 precursor 2,84E-04 1,97E-04 1,437 1,222 0,221734224 1700006E09Rik uncharacterized protein C17orf105 homolog 0,001034708 8,97E-04 1,153 1,222 0,221734224 rpl12 60S ribosomal protein L12 8,58E-04 5,99E-04 1,431 1,222 0,221853043 psmb1 proteasome subunit beta type-1 precursor 6,58E-04 4,74E-04 1,389 1,219 0,222818379 1700061G19Rik uncharacterized protein LOC78625 2,52E-04 3,61E-04 1,432 -1,218 0,223072574 AGPAT3 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma 1,93E-04 9,33E-05 2,069 1,215 0,224370899 RAB10 ras-related protein Rab-10 2,06E-04 1,07E-04 1,915 1,215 0,224370899 pnpla8 calcium-independent phospholipase A2-gamma 3,25E-05 7,39E-05 2,272 -1,208 0,227233082 NDUFAB1 acyl carrier protein, mitochondrial precursor 2,02E-04 4,13E-04 2,049 -1,208 0,227233082

LOC100044117 pyruvate dehydrogenase protein X component, mitochondrial 0,002480871 0,002185123 1,135 1,202 0,229481675 sccpdh saccharopine dehydrogenase-like oxidoreductase 0,003381013 0,003928832 1,162 -1,200 0,230311522 LETM1 and EF-hand domain-containing protein 1, LETM1 mitochondrial precursor 3,67E-04 4,64E-04 1,264 -1,195 0,232140178 PHB2 prohibitin-2 0,002292094 0,002803321 1,223 -1,191 0,233508696 Dnahc7a dynein, axonemal, heavy chain 7B 4,64E-05 6,32E-05 1,362 -1,188 0,23502749 Ppp1r7 protein phosphatase 1 regulatory subunit 7 2,50E-04 3,86E-04 1,543 -1,184 0,236577543 LOC100048601 probable threonine protease PRSS50 precursor 3,73E-04 5,91E-04 1,586 -1,184 0,236577543 Slc25a11 mitochondrial 2-oxoglutarate/malate carrier protein 2,26E-04 3,79E-04 1,673 -1,181 0,237665823 DNPEP aspartyl aminopeptidase isoform b 0,002070523 0,002349307 1,135 -1,174 0,240553336 mitochondrial import inner membrane translocase subunit Gm10171 Tim23 4,86E-04 2,64E-04 1,841 1,169 0,242440991

TSSK4 testis-specific serine/threonine-protein kinase 4 isoform 1 9,21E-04 7,01E-04 1,314 1,161 0,245684408 Igbp1b immunoglobulin-binding protein 1b 9,37E-04 0,001211404 1,292 -1,160 0,246133009 prep prolyl endopeptidase 2,20E-05 5,54E-05 2,519 -1,159 0,246506533 NUDCD2 nudC domain-containing protein 2 1,17E-04 2,80E-04 2,384 -1,159 0,246506533 Gm5428 PREDICTED: 60S ribosomal protein L6-like 4,12E-05 1,18E-04 2,867 -1,159 0,246506533 EFCAB3 EF-hand calcium-binding domain-containing protein 3 3,49E-05 8,64E-05 2,474 -1,159 0,246506533 Erlin2 erlin-2 6,24E-04 8,29E-04 1,328 -1,148 0,251110838 cyb5r3 NADH-cytochrome b5 reductase 3 1,32E-04 2,55E-04 1,929 -1,137 0,255624678 Gnpda2 glucosamine-6-phosphate isomerase 2 1,36E-04 2,57E-04 1,895 -1,137 0,255624678 PDIA3 protein disulfide-isomerase A3 precursor 4,92E-04 4,02E-04 1,226 1,137 0,255653728 rab5c ras-related protein Rab-5C 0,001259646 0,001053306 1,196 1,137 0,255653728 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex Ndufa8 subunit 8 0,002054993 0,001611923 1,275 1,137 0,255653728 lancl1 lanC-like protein 1 2,47E-04 1,37E-04 1,805 1,136 0,255983844 TBL1XR1 F-box-like/WD repeat-containing protein TBL1XR1 1,98E-04 1,14E-04 1,730 1,136 0,255983844 NADH dehydrogenase [ubiquinone] 1 beta subcomplex NDUFB6 subunit 6 5,14E-04 2,94E-04 1,748 1,136 0,255983844 succinate dehydrogenase cytochrome b560 subunit, sdhC mitochondrial precursor 6,44E-04 3,75E-04 1,717 1,136 0,255983844 Pkm pyruvate kinase, muscle isoform M1 0,006395322 0,006761807 1,057 -1,116 0,2644762 CATSPER2 cation channel sperm-associated protein 2 2,51E-04 1,41E-04 1,780 1,112 0,266193589 dhrs1 dehydrogenase/reductase SDR family member 1 2,27E-04 1,38E-04 1,640 1,112 0,266193589 mitochondrial import inner membrane translocase subunit TIMM17B Tim17-B 5,32E-04 3,32E-04 1,603 1,112 0,266193589 4930504O13Rik uncharacterized protein LOC403200 precursor 5,98E-04 3,56E-04 1,681 1,112 0,266193589 BC048507 dynein light chain LC8-type 1-like 7,40E-04 3,99E-04 1,854 1,112 0,266193589 hydroxyacylglutathione hydrolase, mitochondrial isoform 1 HAGH precursor 2,06E-04 3,54E-04 1,721 -1,111 0,266732617 ahcY adenosylhomocysteinase 1,22E-04 2,13E-04 1,748 -1,111 0,266732617 BPI bactericidal permeability-increasing protein precursor 1,57E-04 2,49E-04 1,589 -1,111 0,266732617

SIRT5 NAD-dependent protein deacylase sirtuin-5, mitochondrial 1,86E-04 3,13E-04 1,686 -1,111 0,266732617 Dynll2 dynein light chain 2, cytoplasmic 8,76E-04 0,001210594 1,382 -1,104 0,269378605 Angel1 protein angel homolog 2 isoform 2 1,58E-04 2,66E-04 1,686 -1,104 0,269378605 prcp lysosomal Pro-X carboxypeptidase precursor 4,81E-04 6,44E-04 1,338 -1,099 0,271604874 Tmem190 transmembrane protein 190 precursor 0,002831558 0,002222989 1,274 1,097 0,272579022 KIF9 kinesin-like protein KIF9 isoform 2 1,30E-04 7,69E-05 1,684 1,094 0,273891055 NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, ndufs2 mitochondrial precursor 0,001250118 0,001556297 1,245 -1,091 0,275447162 bat3 large proline-rich protein BAG6 isoform 1 6,06E-04 7,69E-04 1,268 -1,088 0,276674369 NUP155 nuclear pore complex protein Nup155 2,72E-04 1,98E-04 1,378 1,087 0,277048283 NADH dehydrogenase [ubiquinone] 1 beta subcomplex Ndufb5 subunit 5, mitochondrial precursor 0,001097814 8,41E-04 1,306 1,078 0,281202728 CATSPER4 cation channel sperm-associated protein 4 isoform 2 3,90E-04 2,50E-04 1,561 1,072 0,283910346 slc25a1 solute carrier family 25, member 1 3,48E-04 2,38E-04 1,462 1,072 0,283910346 SHMT1 serine hydroxymethyltransferase, cytosolic 2,27E-04 1,51E-04 1,501 1,072 0,283910346 IDH3B isocitrate dehydrogenase 3, beta subunit 0,002057599 0,001614965 1,274 1,068 0,285608488 LOC100046727 IQ and ubiquitin-like domain-containing protein 1,58E-04 1,05E-04 1,511 1,065 0,286964647 Fam71b protein FAM71B 0,002599346 0,002278478 1,141 1,063 0,28789481 UQCRQ cytochrome b-c1 complex subunit 8 0,001939882 0,001322319 1,467 1,063 0,287994978 1700080O16Rik uncharacterized protein LOC74279 4,30E-04 2,99E-04 1,438 1,060 0,289055123 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase PLCD4 delta-4 isoform 1 2,48E-04 3,36E-04 1,355 -1,060 0,28932742 Atp6v1a V-type proton ATPase catalytic subunit A 1,94E-04 2,71E-04 1,397 -1,060 0,28932742 DSTN destrin 1,27E-04 2,34E-04 1,844 -1,059 0,289374377 TSSK6 testis-specific serine/threonine-protein kinase 6 4,42E-04 3,03E-04 1,459 1,057 0,290363139 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia ddt D-dopachrome decarboxylase 9,46E-04 6,34E-04 1,492 1,056 0,291030323 LOC100045699 electron transfer flavoprotein subunit beta 4,26E-04 2,86E-04 1,491 1,056 0,291168752 dihydrolipoyllysine-residue acetyltransferase component of dlaT pyruvate dehydrogenase complex, mitochondrial 0,005802588 0,005934628 1,023 -1,054 0,292101451 Man2b1 lysosomal alpha-mannosidase precursor 4,57E-05 1,99E-05 2,298 1,046 0,295378116 Sirt2 NAD-dependent protein deacetylase sirtuin-2 isoform 1 9,23E-05 4,05E-05 2,278 1,046 0,295378116 MOSC domain-containing protein 2, mitochondrial MOSC2 precursor 9,45E-05 3,85E-05 2,454 1,046 0,295378116 Wfdc6b WAP four-disulfide core domain protein 6B precursor 2,44E-04 1,13E-04 2,164 1,046 0,295378116 - predicted gene 6760 2,59E-04 9,88E-05 2,627 1,046 0,295378116 PHOSPHO1 phosphoethanolamine/phosphocholine phosphatase 8,46E-04 0,001129927 1,335 -1,038 0,299353285 1700026D08Rik uncharacterized protein C15orf26 homolog 0,001219064 9,19E-04 1,326 1,029 0,303518271 Nif3l1 NIF3-like protein 1 1,63E-04 2,82E-04 1,730 -1,028 0,303847568 Gm216 uncharacterized protein C2orf62 homolog 1,55E-04 2,61E-04 1,682 -1,028 0,303847568 Gstt4 glutathione S-transferase theta-4 2,17E-04 3,28E-04 1,512 -1,028 0,303847568 PEBP1 phosphatidylethanolamine-binding protein 1 0,004571822 0,004091267 1,117 1,028 0,304140996 CRISP1 cysteine-rich secretory protein 1 precursor 6,13E-04 8,62E-04 1,406 -1,027 0,304414152 psmb2 proteasome subunit beta type-2 7,15E-04 9,36E-04 1,310 -1,027 0,304414152 4922502D21Rik C-type lectin-like receptor 2m 3,47E-04 5,47E-04 1,576 -1,023 0,306451623 PSMD13 26S proteasome non-ATPase regulatory subunit 13 1,61E-04 2,56E-04 1,592 -1,023 0,306451623 Pgk1-rs7 phosphoglycerate kinase 1 3,96E-04 5,47E-04 1,382 -1,018 0,308486088 Psma4 proteasome subunit alpha type-4 4,75E-04 6,73E-04 1,417 -1,004 0,315597261 Ogdh 2-oxoglutarate dehydrogenase, mitochondrial isoform 1 0,0018471 0,001669461 1,106 0,993 0,320688241 Akr7a5 aflatoxin B1 aldehyde reductase member 2 0,0010769 8,56E-04 1,257 0,991 0,321800427 pyruvate dehydrogenase E1 component subunit alpha, Pdha1 somatic form, mitochondrial precursor 5,85E-04 4,75E-04 1,232 0,991 0,321800427 Fhl4 four and a half LIM domains 4 6,69E-04 8,94E-04 1,337 -0,990 0,322149756 mdh2 malate dehydrogenase, mitochondrial precursor 0,004014748 0,004574706 1,139 -0,989 0,322423649 Acrv1 acrosomal protein SP-10 precursor 0,005284914 0,006702331 1,268 -0,985 0,324828011 Ogdhl 2-oxoglutarate dehydrogenase-like, mitochondrial 2,65E-05 5,40E-05 2,037 -0,978 0,328032346 STUB1 STIP1 homology and U box-containing protein 1 5,98E-05 1,21E-04 2,019 -0,978 0,328032346 transmembrane emp24 domain-containing protein 10 tmed10 precursor 1,43E-04 2,74E-04 1,911 -0,978 0,328032346 cytochrome c oxidase subunit 7A-related protein, Cox7a2l mitochondrial isoform 1 2,07E-04 4,83E-04 2,328 -0,978 0,328032346 Ggct gamma-glutamylcyclotransferase 1,09E-04 2,07E-04 1,895 -0,978 0,328032346 GHITM growth hormone-inducible transmembrane protein 1,23E-04 2,37E-04 1,921 -0,978 0,328032346 RALA ras-related protein Ral-A precursor 1,20E-04 2,55E-04 2,127 -0,978 0,328032346 G-protein coupled receptor family C group 5 member B Gprc5b isoform 2 precursor 1,85E-04 3,72E-04 2,005 -0,978 0,328032346 atad3a ATPase family AAA domain-containing protein 3 3,78E-05 7,67E-05 2,027 -0,978 0,328032346 4933401F05Rik serine protease 55 precursor 3,31E-04 1,69E-04 1,962 0,972 0,331264436 Gm6776 leucine zipper transcription factor-like protein 1 1,43E-04 7,20E-05 1,982 0,972 0,331264436 2310061I04Rik uncharacterized protein LOC69662 1,95E-04 9,93E-05 1,966 0,972 0,331264436 Uchl1 ubiquitin carboxyl-terminal hydrolase isozyme L1 2,08E-04 1,02E-04 2,042 0,972 0,331264436 PREDICTED: mitochondrial import inner membrane Tim14 translocase subunit TIM14-like 2,74E-04 1,34E-04 2,043 0,972 0,331264436 NRD1 nardilysin precursor 4,66E-05 2,29E-05 2,033 0,972 0,331264436

LOC674583 ATP synthase-coupling factor 6, mitochondrial precursor 2,91E-04 1,38E-04 2,100 0,972 0,331264436 Txndc2 thioredoxin domain-containing protein 2 isoform 2 8,29E-05 3,94E-05 2,106 0,972 0,331264436 prmt5 protein arginine N-methyltransferase 5 1,73E-04 2,59E-04 1,496 -0,961 0,336681355 Gm11582 dynein light chain 1, cytoplasmic 0,001096984 0,00162785 1,484 -0,961 0,336681355 Pdia6 PREDICTED: protein disulfide-isomerase A6 2,75E-04 4,04E-04 1,472 -0,961 0,336681355 MORN3 MORN repeat-containing protein 3 2,47E-04 4,09E-04 1,657 -0,944 0,345184141 4930578I06Rik uncharacterized protein C8orf74 homolog 1,59E-04 2,86E-04 1,803 -0,939 0,347928107 Fahd1 acylpyruvase FAHD1, mitochondrial 1,98E-04 3,09E-04 1,558 -0,939 0,347928107 Smpd5 sphingomyelin phosphodiesterase 5 1,38E-04 2,20E-04 1,595 -0,939 0,347928107

Akd1 PREDICTED: adenylate kinase domain-containing protein 1 1,27E-04 1,00E-04 1,264 0,938 0,348341921 DPP4 dipeptidyl peptidase 4 isoform 2 8,82E-05 1,42E-04 1,607 -0,934 0,350239081 CCDC147 coiled-coil domain containing 147 7,46E-04 6,49E-04 1,148 0,932 0,351367236 cutC copper homeostasis protein cutC homolog isoform 2 6,90E-04 5,55E-04 1,241 0,930 0,352136874 Dynlt1b dynein light chain Tctex-type 1F isoform 1 5,68E-04 3,42E-04 1,659 0,927 0,353685861 Gm8219 mitochondrial pyruvate carrier 1 5,46E-04 2,80E-04 1,952 0,927 0,353685861 LOC100047501 40S ribosomal protein S16 2,44E-04 1,35E-04 1,811 0,927 0,353685861 Dnaic1 dynein intermediate chain 1, axonemal 9,93E-04 8,56E-04 1,160 0,927 0,353814603 1700023D19Rik izumo sperm-egg fusion protein 2 precursor 6,86E-04 9,32E-04 1,357 -0,924 0,35554723 Dnah7 PREDICTED: dynein heavy chain 7, axonemal 3,02E-05 4,11E-05 1,364 -0,924 0,35554723 #N/A hemoglobin, beta adult s chain 0,004816249 0,004506586 1,069 0,919 0,358076758 Aldoa1b aldolase 1 A retrogene 2 0,00106529 0,001391983 1,307 -0,915 0,360335302 solute carrier family 2, facilitated glucose transporter SLC2A3 member 3 0,00542572 0,004831955 1,123 0,907 0,364295293 echdc1 ethylmalonyl-CoA decarboxylase isoform a 1,80E-04 1,09E-04 1,650 0,900 0,368106121 ASB9 ankyrin repeat and SOCS box protein 9 3,48E-04 2,00E-04 1,742 0,900 0,368106121 CATSPER1 cation channel sperm-associated protein 1 2,11E-04 1,30E-04 1,631 0,900 0,368106121 eIF6 eukaryotic translation initiation factor 6 3,66E-04 2,11E-04 1,734 0,900 0,368106121 low molecular weight phosphotyrosine protein phosphatase acp1 isoform 2 3,24E-04 1,87E-04 1,733 0,900 0,368106121 Ptgr2 prostaglandin reductase 2 isoform 1 2,04E-04 1,28E-04 1,595 0,900 0,368106121 ARHGDIA rho GDP-dissociation inhibitor 1 3,01E-04 1,93E-04 1,563 0,900 0,368106121 stom erythrocyte band 7 integral membrane protein 0,001611922 0,00137285 1,174 0,898 0,369189401 Mdh1 malate dehydrogenase, cytoplasmic 2,87E-04 4,14E-04 1,442 -0,898 0,369418442 NME5 nucleoside diphosphate kinase homolog 5 9,96E-04 7,17E-04 1,391 0,896 0,37039812 PRDX2 peroxiredoxin-2 6,99E-04 5,74E-04 1,217 0,896 0,37039812 CMPK2 UMP-CMP kinase 2, mitochondrial precursor 3,85E-05 8,40E-05 2,183 -0,894 0,371198877 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia SEPHS2 selenide, water dikinase 2 8,22E-05 1,70E-04 2,074 -0,894 0,371198877 NUDT2 bis(5-nucleosyl)-tetraphosphatase [asymmetrical] 9,21E-05 1,99E-04 2,162 -0,894 0,371198877 Gm7684 E3 ubiquitin-protein ligase MARCH5 isoform 1 5,81E-05 1,24E-04 2,132 -0,894 0,371198877 1700024G13Rik UPF0728 protein C10orf53 homolog 1,65E-04 3,47E-04 2,098 -0,894 0,371198877 Aldoa fructose-bisphosphate aldolase A isoform 1 precursor 0,001382077 0,001656066 1,198 -0,892 0,372132078 TCP1 T-complex protein 1 subunit alpha 0,00118338 0,001056226 1,120 0,889 0,374145515 LOC630540 WD repeat domain 63 2,40E-04 3,10E-04 1,290 -0,887 0,37504137 Hmox2 heme oxygenase 2 4,61E-04 3,45E-04 1,336 0,884 0,376954426 4932425I24Rik protein MAATS1 9,18E-05 5,87E-05 1,565 0,883 0,377411087 PPP6C serine/threonine-protein phosphatase 6 catalytic subunit 2,03E-04 1,24E-04 1,644 0,883 0,377411087 1700001C19Rik uncharacterized protein LOC75462 isoform 2 4,69E-04 2,99E-04 1,567 0,883 0,377411087 Phpt1 14 kDa phosphohistidine phosphatase 4,14E-04 2,83E-04 1,464 0,883 0,377411087 KRT17 keratin, type I cytoskeletal 17 1,76E-04 1,11E-04 1,577 0,883 0,377411087 GPX6 glutathione peroxidase 6 precursor 4,08E-04 2,77E-04 1,473 0,883 0,377411087 disintegrin and metalloproteinase domain-containing protein Adam1b 1b precursor 2,65E-04 2,03E-04 1,301 0,878 0,379931419 CCDC39 coiled-coil domain-containing protein 39 2,56E-04 3,03E-04 1,186 -0,874 0,382281729 glycerophosphodiester phosphodiesterase domain-containing Gdpd4 protein 4 4,13E-04 3,06E-04 1,347 0,873 0,382632693 CES3 carboxylesterase 1D precursor 1,76E-04 1,17E-04 1,506 0,872 0,383267528 vps25 vacuolar protein-sorting-associated protein 25 3,93E-04 2,70E-04 1,454 0,872 0,383267528 SLC22A14 solute carrier family 22 member 14 2,18E-04 1,37E-04 1,588 0,872 0,383267528 4732415M23Rik uncharacterized protein C16orf55 homolog 6,20E-04 4,40E-04 1,409 0,866 0,386705218 1700009N14Rik uncharacterized protein LOC75471 2,99E-04 2,11E-04 1,417 0,866 0,386705218 LCN12 epididymal-specific lipocalin-12 precursor 0,001122518 8,09E-04 1,387 0,865 0,386884174 PPAP2A lipid phosphate phosphohydrolase 1 isoform 1 6,75E-04 4,72E-04 1,431 0,863 0,388209601 Tceb2 transcription elongation factor B polypeptide 2 7,54E-04 5,07E-04 1,487 0,863 0,38839438 Nup53 nucleoporin NUP53 isoform 2 4,13E-04 3,07E-04 1,347 0,863 0,38839438 SEC11A signal peptidase complex catalytic subunit SEC11A 4,18E-04 3,09E-04 1,355 0,863 0,38839438 DDI1 protein DDI1 homolog 1 3,80E-04 2,59E-04 1,469 0,862 0,388789587 PSMB5 proteasome subunit beta type-5 0,001558533 0,001341194 1,162 0,859 0,390118171 Ces7 carboxylesterase 5A precursor 5,22E-04 6,78E-04 1,298 -0,854 0,393072107 Arpm1 actin-related protein T3 4,61E-04 6,16E-04 1,336 -0,854 0,393072107 BPGM bisphosphoglycerate mutase 6,12E-04 7,56E-04 1,236 -0,854 0,393072107 1700007I08Rik actin-like protein 10 2,33E-04 3,60E-04 1,546 -0,851 0,394630888 CAPZA3 F-actin-capping protein subunit alpha-3 8,42E-04 6,51E-04 1,292 0,851 0,394943289 DNAJC10 dnaJ homolog subfamily C member 10 precursor 4,54E-05 8,02E-05 1,765 -0,840 0,401182763 THOP1 thimet oligopeptidase 4,87E-05 8,13E-05 1,668 -0,840 0,401182763 Gm5429 uncharacterized protein C7orf72 homolog 2,76E-04 4,82E-04 1,750 -0,840 0,401182763 Rab1 ras-related protein Rab-1A 1,23E-04 2,07E-04 1,678 -0,840 0,401182763 Gm5081 ribose-phosphate pyrophosphokinase 1 1,06E-04 1,80E-04 1,696 -0,840 0,401182763 snap23 synaptosomal-associated protein 23 isoform a 1,81E-04 2,81E-04 1,555 -0,837 0,402677262 Styxl1 serine/threonine/tyrosine-interacting-like protein 1 1,63E-04 2,41E-04 1,479 -0,837 0,402677262 GLUL glutamine synthetase 0,001114206 0,001431574 1,285 -0,830 0,40627263 uncharacterized coiled-coil domain-containing protein 4921530D09Rik KIAA1984 7,91E-04 6,75E-04 1,172 0,830 0,406772532 LOC674469 ATP synthase subunit d, mitochondrial 0,002673368 0,003109368 1,163 -0,822 0,411317203 Dnahc17 dynein heavy chain 17, axonemal 4,03E-04 4,04E-04 1,002 -0,819 0,412820064 them4 acyl-coenzyme A thioesterase THEM4 8,90E-04 0,001125174 1,265 -0,804 0,421190194 1700071K01Rik prohibitin 0,002307591 0,002595415 1,125 -0,797 0,425646251 NADH dehydrogenase [ubiquinone] 1 beta subcomplex Ndufb8 subunit 8, mitochondrial precursor 0,001440395 0,00126805 1,136 0,795 0,426412822 CYLC2 cylicin, basic protein of sperm head cytoskeleton 2 6,92E-04 6,12E-04 1,131 0,795 0,426412822

TEPP testis, prostate and placenta-expressed protein isoform 1 0,001329739 0,00121632 1,093 0,795 0,426412822 cct6a T-complex protein 1 subunit zeta 5,27E-04 6,23E-04 1,182 -0,782 0,434125864 Actrt2 actin-related protein T2 2,06E-04 2,84E-04 1,376 -0,777 0,436992777 Cfl2 cofilin-2 3,09E-04 4,42E-04 1,429 -0,777 0,436992777 morn5 MORN repeat-containing protein 5 7,41E-04 9,39E-04 1,266 -0,772 0,439849658 Uchl3 ubiquitin carboxyl-terminal hydrolase isozyme L3 7,56E-04 9,64E-04 1,275 -0,767 0,443137337 ccdc65 coiled-coil domain-containing protein 65 3,38E-04 2,70E-04 1,250 0,759 0,447632315 HSPA9 stress-70 protein, mitochondrial 0,001724229 0,001881744 1,091 -0,753 0,451588128 Ado 2-aminoethanethiol dioxygenase 2,23E-04 3,57E-04 1,603 -0,749 0,453750112 MYCBP C-Myc-binding protein 4,56E-04 6,69E-04 1,465 -0,749 0,453750112 Rnls renalase isoform 1 0,004247023 0,004596706 1,082 -0,737 0,461248525 SYNGR4 synaptogyrin-4 0,003253489 0,004050254 1,245 -0,735 0,46248991 LTA4H leukotriene A-4 hydrolase 7,20E-05 1,05E-04 1,457 -0,728 0,466712606 SPCS3 signal peptidase complex subunit 3 2,61E-04 3,94E-04 1,508 -0,728 0,466712606 SSNA1 Sjoegren syndrome nuclear autoantigen 1 homolog 2,93E-04 4,35E-04 1,488 -0,728 0,466712606 cetn1 centrin-1 2,23E-04 3,27E-04 1,464 -0,728 0,466712606 PREDICTED: up-regulated during skeletal muscle growth - protein 5-like 6,89E-04 0,001270842 1,844 -0,728 0,466712606 LOC675851 NADH dehydrogenase [ubiquinone] 1 subunit C2 0,001257071 0,001116575 1,126 0,727 0,466968853 rdh11 retinol dehydrogenase 11 precursor 9,39E-05 1,59E-04 1,691 -0,727 0,467209604 Mdh1b putative malate dehydrogenase 1B 6,52E-05 1,05E-04 1,614 -0,727 0,467209604 Pdia4 protein disulfide-isomerase A4 precursor 3,50E-05 6,14E-05 1,752 -0,727 0,467209604 CIB1 calcium and integrin-binding protein 1 1,07E-04 1,82E-04 1,700 -0,727 0,467209604 PLP1 myelin proteolipid protein 1,37E-04 2,56E-04 1,870 -0,727 0,467209604 EFCAB10 EF-hand calcium-binding domain-containing protein 10 2,32E-04 3,73E-04 1,609 -0,727 0,467209604 LOC100046792 uncharacterized protein C6orf163 homolog 4,13E-04 5,26E-04 1,274 -0,726 0,467605395 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex Ndufa13 subunit 13 0,001882751 0,001577929 1,193 0,721 0,47120977 1700019N19Rik uncharacterized protein C10orf82 homolog 0,001456851 0,001223709 1,191 0,716 0,473918242 uqcrfs1 cytochrome b-c1 complex subunit Rieske, mitochondrial 0,002996527 0,003311531 1,105 -0,712 0,476761689 Apoh beta-2-glycoprotein 1 precursor 2,21E-04 3,01E-04 1,362 -0,711 0,477369703 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia PREDICTED: NADH dehydrogenase [ubiquinone] iron- Ndufs5 sulfur protein 5-like 6,30E-04 7,70E-04 1,222 -0,711 0,477369703 1700020C11Rik mitochondrial fission process protein 1 8,52E-04 6,55E-04 1,300 0,695 0,487013653 BC048562 uncharacterized protein LOC434439 0,00108912 8,63E-04 1,262 0,695 0,487013653 Gm1060 dynein regulatory complex protein 1 2,46E-04 3,09E-04 1,255 -0,688 0,491674217 STX12 syntaxin-12 6,13E-04 8,41E-04 1,372 -0,686 0,492771583 electron transfer flavoprotein-ubiquinone oxidoreductase, Etfdh mitochondrial precursor 2,12E-04 1,65E-04 1,286 0,684 0,493787278 Naglu alpha-N-acetylglucosaminidase precursor 9,87E-04 9,03E-04 1,093 0,675 0,499634278 1700007K13Rik UPF0691 protein C9orf116 homolog 5,49E-04 7,96E-04 1,450 -0,672 0,501407707 IMMP1L mitochondrial inner membrane protease subunit 1 6,20E-04 4,82E-04 1,286 0,663 0,507299268 Nme1 nucleoside diphosphate kinase A 7,43E-04 5,52E-04 1,345 0,663 0,507299268 Spesp1 sperm equatorial segment protein 1 precursor 0,008001396 0,009194719 1,149 -0,656 0,511762262 calcium-binding tyrosine phosphorylation-regulated protein Cabyr isoform c 9,29E-05 4,72E-05 1,967 0,656 0,511953211 HSPA4 heat shock 70 kDa protein 4 2,80E-05 1,57E-05 1,782 0,656 0,511953211 LOC100045772 multidrug resistance-associated protein 9 2,47E-05 1,36E-05 1,810 0,656 0,511953211 Abcg2 ATP-binding cassette sub-family G member 2 6,04E-05 3,28E-05 1,842 0,656 0,511953211 Krt42 keratin, type I cytoskeletal 42 6,42E-05 3,39E-05 1,890 0,656 0,511953211 ERP44 endoplasmic reticulum resident protein 44 precursor 7,25E-05 4,03E-05 1,801 0,656 0,511953211 LOC100045901 PREDICTED: ubiquitin-conjugating enzyme E2 L3-like 1,74E-04 1,10E-04 1,582 0,656 0,511953211 PSMC2 26S protease regulatory subunit 7 3,19E-04 2,64E-04 1,208 0,654 0,513310984 Spam1 hyaluronidase PH-20 precursor 8,75E-04 7,96E-04 1,099 0,653 0,513513353 succinate dehydrogenase [ubiquinone] flavoprotein subunit, SDHA mitochondrial precursor 0,002819026 0,002725666 1,034 0,651 0,515012013 9030607L17Rik izumo sperm-egg fusion protein 4 precursor 0,003361152 0,003400782 1,012 0,651 0,515294552 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex Ndufa2 subunit 2 6,60E-04 8,07E-04 1,223 -0,650 0,515845722 SPAG16 sperm-associated antigen 16 protein isoform 1 1,66E-04 2,26E-04 1,366 -0,650 0,515845722 Ywhaz 14-3-3 protein zeta/delta isoform 1 0,001708833 0,001972862 1,155 -0,645 0,518985132 Lrrc57 leucine-rich repeat-containing protein 57 isoform b 4,37E-04 5,90E-04 1,349 -0,642 0,52113851 Trap1 heat shock protein 75 kDa, mitochondrial precursor 1,85E-04 2,34E-04 1,264 -0,641 0,521616628 MPI mannose-6-phosphate isomerase 4,18E-04 5,29E-04 1,263 -0,641 0,521616628 ATP1A4 sodium/potassium-transporting ATPase subunit alpha-4 0,001962105 0,002111607 1,076 -0,640 0,522448049 DNALI1 axonemal dynein light intermediate polypeptide 1 0,002106784 0,002401976 1,140 -0,638 0,523674135 mycophenolic acid acyl-glucuronide esterase, mitochondrial ABHD10 isoform 1 precursor 7,12E-04 8,61E-04 1,208 -0,636 0,524621201 CCDC96 coiled-coil domain-containing protein 96 8,16E-05 1,21E-04 1,485 -0,635 0,525674892 psmc3 26S protease regulatory subunit 6A 9,67E-05 1,35E-04 1,396 -0,635 0,525674892 4921536K21Rik uncharacterized protein C5orf52 homolog 4,44E-04 3,53E-04 1,258 0,633 0,526528027 Gm128 protein MENT precursor 0,008122374 0,009328123 1,148 -0,630 0,528914235 Echs1 enoyl-CoA hydratase, mitochondrial precursor 6,49E-04 5,58E-04 1,163 0,627 0,530668623 rpl8 60S ribosomal protein L8 3,08E-04 2,47E-04 1,248 0,625 0,532173902 9230107M04Rik binder of sperm protein homolog 2 precursor 3,00E-04 2,22E-04 1,353 0,624 0,532545743 arl1 ADP-ribosylation factor-like protein 1 1,88E-04 1,10E-04 1,698 0,624 0,532545743 ART3 ecto-ADP-ribosyltransferase 3 precursor 1,17E-04 7,28E-05 1,602 0,624 0,532545743 protein disulfide-isomerase-like protein of the testis Pdilt precursor 5,62E-05 3,50E-05 1,606 0,624 0,532545743 thioredoxin-dependent peroxide reductase, mitochondrial prdx3 precursor 1,49E-04 9,72E-05 1,531 0,624 0,532545743 dihydroorotate dehydrogenase (quinone), mitochondrial dhodh precursor 8,98E-05 5,80E-05 1,548 0,624 0,532545743 ME1 NADP-dependent malic enzyme isoform 1 6,76E-04 7,52E-04 1,113 -0,620 0,535551547

C8orfk29 PREDICTED: putative transmembrane protein C8orfK29 2,42E-04 1,80E-04 1,350 0,617 0,537038714 PPM1B protein phosphatase 1B isoform 1 1,44E-04 1,13E-04 1,272 0,617 0,537038714 pgam1 phosphoglycerate mutase 1 1,75E-04 1,10E-04 1,588 0,612 0,540504945 Antxrl anthrax toxin receptor-like precursor 9,19E-05 6,16E-05 1,493 0,612 0,540504945 gpX3 glutathione peroxidase 3 precursor 2,00E-04 1,38E-04 1,455 0,612 0,540504945 glycerophosphodiester phosphodiesterase domain-containing gdpd1 protein 1 1,17E-04 8,20E-05 1,424 0,612 0,540504945 long-chain specific acyl-CoA dehydrogenase, mitochondrial acadl precursor 9,66E-05 6,30E-05 1,533 0,612 0,540504945 NUDT3 diphosphoinositol polyphosphate phosphohydrolase 1 2,62E-04 1,72E-04 1,522 0,612 0,540504945 Gbas protein NipSnap homolog 2 2,00E-04 1,38E-04 1,447 0,612 0,540504945 PSMD14 26S proteasome non-ATPase regulatory subunit 14 2,89E-04 1,95E-04 1,486 0,612 0,540665662 nsdhl sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating 2,13E-04 1,56E-04 1,362 0,612 0,540665662 - uncharacterized protein LOC627607 1,07E-04 7,29E-05 1,467 0,609 0,542284995 putative transferase CAF17 homolog, mitochondrial isoform A230051G13Rik 1 precursor 1,71E-04 1,25E-04 1,370 0,609 0,542284995 Gm9840 E3 ubiquitin-protein ligase RBX1 6,26E-04 4,75E-04 1,317 0,609 0,542284995 GSTO2 glutathione S-transferase omega-2 0,001170244 0,001046446 1,118 0,607 0,54368571 AMDHD2 putative N-acetylglucosamine-6-phosphate deacetylase 2,37E-04 3,05E-04 1,290 -0,604 0,545949033 peptidyl-prolyl cis-trans isomerase F, mitochondrial PPIF precursor 1,25E-04 1,96E-04 1,574 -0,602 0,547246735 Gm7655 isopentenyl-diphosphate Delta-isomerase 1 1,20E-04 1,64E-04 1,370 -0,602 0,547246735 carcinoembryonic antigen-related cell adhesion molecule 10 Ceacam10 precursor 2,43E-04 3,30E-04 1,358 -0,602 0,547246735 EFCAB1 EF-hand calcium-binding domain-containing protein 1 1,48E-04 2,19E-04 1,484 -0,602 0,547246735 complement component 1 Q subcomponent-binding protein, c1qbp mitochondrial 8,29E-04 7,32E-04 1,133 0,599 0,54911051 dci enoyl-CoA delta isomerase 1, mitochondrial precursor 6,28E-04 5,13E-04 1,223 0,599 0,54911051 CCDC81 coiled-coil domain-containing protein 81 2,01E-04 2,54E-04 1,265 -0,598 0,549669944 NADH dehydrogenase [ubiquinone] flavoprotein 2, Ndufv2 mitochondrial isoform 1 precursor 0,002059612 0,002342213 1,137 -0,596 0,550892645 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia 4930579C15Rik equatorin precursor 0,002434144 0,002197992 1,107 0,596 0,551099674 BC061194 uncharacterized protein LOC381350 2,33E-04 3,14E-04 1,347 -0,594 0,552523263

Tmco2 transmembrane and coiled-coil domain-containing protein 2 8,64E-04 0,001048317 1,213 -0,594 0,552523263

SPACA3 sperm acrosome membrane-associated protein 3 precursor 9,07E-04 0,001056748 1,165 -0,594 0,552764659 psma5 proteasome subunit alpha type-5 6,14E-04 7,51E-04 1,222 -0,594 0,552764659 ppiB peptidyl-prolyl cis-trans isomerase B precursor 4,23E-04 5,58E-04 1,318 -0,594 0,552764659 1700011E24Rik uncharacterized protein C2orf61 homolog 5,61E-05 1,01E-04 1,810 -0,594 0,552775748 tmem38b trimeric intracellular cation channel type B 8,01E-05 1,22E-04 1,523 -0,594 0,552775748 SLC13A5 solute carrier family 13 member 5 6,58E-05 1,05E-04 1,601 -0,594 0,552775748 Ubl4 ubiquitin-like protein 4A 8,93E-05 1,61E-04 1,799 -0,594 0,552775748 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial ech1 precursor 5,05E-05 8,14E-05 1,611 -0,594 0,552775748 Tmprss12 transmembrane protease serine 12 precursor 6,78E-05 1,13E-04 1,666 -0,594 0,552775748

2310028O11Rik mitochondrial inner membrane organizing system protein 1 2,43E-04 3,77E-04 1,551 -0,594 0,552775748 pyruvate dehydrogenase E1 component subunit alpha, testis- pdhA2 specific form, mitochondrial precursor 0,007585223 0,007200256 1,053 0,592 0,553863813 CCDC63 coiled-coil domain-containing protein 63 9,73E-04 0,001076706 1,107 -0,591 0,554403314 ASRGL1 isoaspartyl peptidase/L-asparaginase 0,001637608 0,001535239 1,067 0,589 0,555619932

dihydrolipoyllysine-residue succinyltransferase component of DLST 2-oxoglutarate dehydrogenase complex, mitochondrial 0,001230243 0,001393699 1,133 -0,589 0,555825597 CISD1 CDGSH iron-sulfur domain-containing protein 1 0,00186544 0,002194542 1,176 -0,581 0,561396585 GAS8 growth arrest-specific protein 8 4,35E-04 3,74E-04 1,164 0,579 0,562360015 NADH dehydrogenase [ubiquinone] 1 beta subcomplex NDUFB10 subunit 10 0,001005548 8,87E-04 1,134 0,579 0,562360015 spata18 mitochondria-eating protein 0,002399523 0,002173621 1,104 0,566 0,571503129 coiled-coil-helix-coiled-coil-helix domain-containing protein CHCHD3 3, mitochondrial precursor 0,001079432 9,47E-04 1,140 0,562 0,574271231 cyc1 cytochrome c1, heme protein, mitochondrial 0,002751021 0,003174415 1,154 -0,560 0,575509212 ALDH2 aldehyde dehydrogenase, mitochondrial precursor 1,14E-04 1,49E-04 1,311 -0,555 0,579058088 ccdc108 coiled-coil domain-containing protein 108 5,02E-05 6,55E-05 1,306 -0,555 0,579058088 PPM1A protein phosphatase 1A 3,61E-04 3,15E-04 1,147 0,555 0,579143559 1700012P22Rik uncharacterized protein C1orf158 homolog 0,001073243 9,30E-04 1,154 0,550 0,582208289 amyotrophic lateral sclerosis 2 (juvenile) chromosome Als2cr11 region, candidate 11 2,62E-04 3,28E-04 1,248 -0,549 0,583278529 Gm2972 ATP synthase subunit e, mitochondrial 0,001291223 0,001611399 1,248 -0,549 0,583278529 Dynlrb2 dynein light chain roadblock-type 2 9,23E-04 0,001193492 1,293 -0,542 0,587511255 PPA2 inorganic pyrophosphatase 2, mitochondrial precursor 3,69E-04 3,20E-04 1,153 0,539 0,589884066 4931409K22Rik IQ and AAA domain-containing protein 1-like 1,47E-04 1,24E-04 1,181 0,539 0,589884066 Fh1 fumarate hydratase, mitochondrial precursor 6,91E-04 8,15E-04 1,180 -0,538 0,590673912 probable isocitrate dehydrogenase [NAD] gamma 2, 4933405O20Rik mitochondrial 8,68E-04 7,53E-04 1,153 0,533 0,593766217 1700001C02Rik UPF0573 protein C2orf70 homolog 0,00119726 0,00141031 1,178 -0,532 0,594417214 Capzb F-actin-capping protein subunit beta isoform c 0,001407936 0,001279883 1,100 0,526 0,59913436 Wdr52 WD repeat-containing protein 52 2,74E-04 2,56E-04 1,070 0,523 0,601124475 1700101E01Rik uncharacterized protein LOC329375 isoform 1 5,52E-04 6,76E-04 1,226 -0,519 0,603465281 RANBP17 ran-binding protein 17 5,06E-04 4,66E-04 1,085 0,510 0,609777792 lrrc48 leucine-rich repeat-containing protein 48 3,14E-04 3,74E-04 1,192 -0,506 0,612726266 1700019L03Rik uncharacterized protein C9orf117 homolog 8,06E-05 1,07E-04 1,323 -0,502 0,615390063 Actc1 actin, alpha cardiac muscle 1 7,98E-05 1,21E-04 1,511 -0,502 0,615390063 Dguok deoxyguanosine kinase, mitochondrial isoform 1 1,62E-04 2,18E-04 1,343 -0,502 0,615390063 Acsl6 long-chain-fatty-acid--CoA ligase 6 isoform 1 5,88E-05 7,85E-05 1,336 -0,502 0,615390063 1700080E11Rik uncharacterized protein LOC73532 1,72E-04 2,26E-04 1,317 -0,502 0,615390063 succinyl-CoA:3-ketoacid coenzyme A transferase 1, oxct1 mitochondrial precursor 3,64E-04 4,31E-04 1,185 -0,500 0,617408475 APOOL apolipoprotein O-like precursor 3,63E-04 2,94E-04 1,234 0,489 0,624754281 PSMB6 proteasome subunit beta type-6 precursor 5,19E-04 4,42E-04 1,176 0,489 0,624754281 IMPA1 inositol monophosphatase 1 8,96E-04 7,63E-04 1,173 0,488 0,625215081 MTX2 metaxin-2 0,001073087 9,91E-04 1,082 0,487 0,626083945 putative ATP-dependent Clp protease proteolytic subunit, clpP mitochondrial precursor 7,39E-04 7,00E-04 1,057 0,487 0,626083945 [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase Pdp1 1, mitochondrial isoform b 1,39E-04 1,73E-04 1,252 -0,485 0,627703986 LYZL4 lysozyme-like protein 4 precursor 4,89E-04 6,78E-04 1,388 -0,485 0,627703986 pcmt1 protein-L-isoaspartate(D-aspartate) O-methyltransferase 2,43E-04 3,17E-04 1,305 -0,485 0,627703986 Tmem177 transmembrane protein 177 2,44E-04 3,32E-04 1,358 -0,485 0,627703986 BANF1 barrier-to-autointegration factor 6,42E-04 8,07E-04 1,257 -0,485 0,627703986 probable inactive protein kinase-like protein SgK071 Gm711 homolog 1,77E-04 2,12E-04 1,202 -0,485 0,627703986 1700071K01Rik prohibitin-like 5,37E-04 6,35E-04 1,183 -0,483 0,629247182 TEKT4 tektin-4 9,13E-04 8,78E-04 1,040 0,482 0,630153139 CCT8 T-complex protein 1 subunit theta 5,62E-04 6,21E-04 1,106 -0,478 0,632505667 4921511H03Rik uncharacterized protein C7orf62 homolog 5,32E-04 4,29E-04 1,238 0,471 0,637407631 mitochondrial import inner membrane translocase subunit timm50 TIM50 precursor 5,86E-04 5,59E-04 1,049 0,470 0,63807659 LOC100046510 dynein heavy chain 8, axonemal 4,21E-04 4,25E-04 1,010 -0,470 0,638700377 NHEDC1 sodium/hydrogen exchanger 9B1 5,87E-04 6,67E-04 1,136 -0,467 0,640557106 PSMA8 proteasome subunit alpha type-7-like 7,01E-04 8,12E-04 1,159 -0,467 0,640557106 WDR64 WD repeat-containing protein 64 1,46E-04 1,75E-04 1,194 -0,466 0,641155342 PRSS21 testisin precursor 0,003101343 0,003042901 1,019 0,463 0,643607233 csnk1a1 casein kinase I isoform alpha 6,97E-04 6,27E-04 1,111 0,457 0,647857182 Cct7 T-complex protein 1 subunit eta 6,72E-04 7,26E-04 1,081 -0,456 0,648155852 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia PPP3R2 calcineurin subunit B type 2 4,81E-04 4,12E-04 1,166 0,453 0,650670251 Arl3 ADP-ribosylation factor-like protein 3 5,12E-04 4,01E-04 1,277 0,453 0,650670251 Spa17 sperm surface protein Sp17 0,001619082 0,001519277 1,066 0,453 0,650773747 Gm4045 ATP synthase subunit g, mitochondrial 0,001321867 0,001624239 1,229 -0,450 0,652849556 Gm10078 28S ribosomal protein S36, mitochondrial isoform 1 4,27E-04 6,71E-04 1,571 -0,449 0,653628166 eif5a eukaryotic translation initiation factor 5A-1 2,37E-04 2,78E-04 1,170 -0,449 0,653628166 CALR calreticulin precursor 7,20E-05 1,03E-04 1,429 -0,449 0,653628166 NADH dehydrogenase [ubiquinone] flavoprotein 3, NDUFV3 mitochondrial isoform 2 1,91E-04 2,69E-04 1,410 -0,449 0,653628166 dcaf7 DDB1- and CUL4-associated factor 7 8,55E-05 1,22E-04 1,428 -0,449 0,653628166 LOC674674 farnesyl pyrophosphate synthase isoform 2 8,50E-05 1,17E-04 1,379 -0,449 0,653628166 TCEB1 transcription elongation factor B polypeptide 1 2,10E-04 3,01E-04 1,431 -0,449 0,653628166 1700094D03Rik uncharacterized protein C1orf189 homolog 2,15E-04 2,78E-04 1,293 -0,449 0,653628166 Rexo2 oligoribonuclease, mitochondrial precursor 9,98E-05 1,31E-04 1,316 -0,449 0,653628166 Dnaic2 dynein intermediate chain 2, axonemal 6,60E-04 5,92E-04 1,115 0,439 0,660678545 PSMA1 proteasome subunit alpha type-1 9,15E-04 0,001014368 1,108 -0,436 0,663176215 Gm2962 cytochrome b-c1 complex subunit 7 0,001044334 8,83E-04 1,182 0,435 0,663530543 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, NDUFS4 mitochondrial 6,77E-04 6,46E-04 1,047 0,435 0,663530543 serine/threonine-protein phosphatase 2A catalytic subunit PPP2CB beta isoform 3,30E-04 3,92E-04 1,189 -0,435 0,663718003 CCIN calicin 0,001505647 0,001413515 1,065 0,434 0,664341428 Uqcrh cytochrome b-c1 complex subunit 6, mitochondrial 9,26E-04 7,57E-04 1,223 0,434 0,664616284 Gm6293 cytochrome b-c1 complex subunit 9 0,001483799 0,001375396 1,079 0,434 0,664616284 Odf2 outer dense fiber protein 2 isoform e 0,00435 0,004393161 1,010 -0,433 0,664808518 Gm3982 mitochondrial pyruvate carrier 2 0,005065002 0,004985642 1,016 0,431 0,666427398

Gm5436 PREDICTED: ATP synthase subunit O, mitochondrial-like 0,002787541 0,003181558 1,141 -0,431 0,666709069 Dnalc1 dynein light chain 1, axonemal 6,84E-04 8,44E-04 1,234 -0,428 0,668613156 Sept10 septin-10 isoform 1 3,74E-04 4,35E-04 1,162 -0,428 0,668613156 MNS1 meiosis-specific nuclear structural protein 1 4,43E-04 5,08E-04 1,147 -0,422 0,673340688 3110056O03Rik signal peptide peptidase-like 2B precursor 1,52E-04 2,14E-04 1,409 -0,420 0,674501493 CCDC151 coiled-coil domain-containing protein 151 isoform 2 4,40E-04 4,94E-04 1,122 -0,418 0,676064801 cbr4 carbonyl reductase family member 4 0,001201316 0,001357221 1,130 -0,418 0,676064801 Ace3 angiotensin I converting enzyme 3 precursor 9,22E-04 0,001051958 1,141 -0,417 0,67638939

GAPDHS glyceraldehyde-3-phosphate dehydrogenase, testis-specific 0,006845997 0,007178342 1,049 -0,417 0,676544142 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA11 subunit 11 8,59E-04 6,54E-04 1,314 0,413 0,679336868 C1orf101 PREDICTED: uncharacterized protein C1orf101-like 1,29E-04 1,05E-04 1,222 0,413 0,679336868

DHRS7B dehydrogenase/reductase SDR family member 7B isoform 2 4,69E-04 4,25E-04 1,105 0,397 0,691250062 Cox6b2 cytochrome c oxidase subunit 6B2 isoform 2 0,001661682 0,001393207 1,193 0,397 0,691250062 Cops8 COP9 signalosome complex subunit 8 5,41E-04 4,56E-04 1,186 0,392 0,694946221 NADH dehydrogenase [ubiquinone] 1 beta subcomplex NDUFB3 subunit 3 6,08E-04 4,97E-04 1,224 0,392 0,694946221 Arl8b ADP-ribosylation factor-like protein 8B 3,51E-04 2,87E-04 1,223 0,392 0,694946221 GSTT3 glutathione S-transferase, theta 3 3,76E-04 2,91E-04 1,293 0,392 0,694946221 PSMD6 26S proteasome non-ATPase regulatory subunit 6 1,57E-04 1,27E-04 1,235 0,392 0,694946221 KLHL10 kelch-like protein 10 2,44E-04 2,83E-04 1,160 -0,392 0,695146121 Hspa1l heat shock 70 kDa protein 1-like 0,001118638 0,001093426 1,023 0,390 0,696644978 LOC100047577 cytochrome b5 type B precursor 0,002243288 0,002030367 1,105 0,389 0,69759251 ribC1 RIB43A-like with coiled-coils protein 1 4,79E-04 4,22E-04 1,135 0,388 0,697987611 Lrrc18 leucine-rich repeat-containing protein 18 isoform 1 3,36E-04 4,36E-04 1,298 -0,387 0,698514609 SLC8A2 sodium/calcium exchanger 2 precursor 2,02E-04 2,31E-04 1,143 -0,387 0,698514609 Usp7 ubiquitin carboxyl-terminal hydrolase 7 1,93E-04 2,17E-04 1,120 -0,382 0,702401661 Sept12 septin-12 isoform 1 4,67E-04 4,29E-04 1,087 0,377 0,706103131 Gm7204 peroxiredoxin-1 5,67E-04 5,19E-04 1,093 0,377 0,706103131 Dbil5 diazepam-binding inhibitor-like 5 0,002043386 0,001811525 1,128 0,372 0,710215682 HSP90AA1 heat shock protein HSP 90-alpha 4,67E-04 5,06E-04 1,083 -0,370 0,711306387 dnaJ homolog subfamily A member 3, mitochondrial isoform DNAJA3 1 1,28E-04 1,10E-04 1,160 0,370 0,711590873 NADH dehydrogenase [ubiquinone] 1 beta subcomplex NDUFB11 subunit 11, mitochondrial 4,46E-04 3,74E-04 1,191 0,370 0,711590873

Spaca4 sperm acrosome membrane-associated protein 4 precursor 0,001446927 0,001723134 1,191 -0,368 0,712673362 ATP6V1E2 V-type proton ATPase subunit E 2 3,04E-04 3,65E-04 1,203 -0,367 0,713437145 isocitrate dehydrogenase [NAD] subunit alpha, idh3a mitochondrial precursor 0,001500673 0,001598533 1,065 -0,365 0,715064805 LOC100048331 dnaJ homolog subfamily A member 4 3,00E-04 2,73E-04 1,099 0,356 0,721715353 macrod1 O-acetyl-ADP-ribose deacetylase MACROD1 1,62E-04 2,08E-04 1,280 -0,350 0,726586945 APEH acylamino-acid-releasing enzyme 1,10E-04 1,29E-04 1,177 -0,350 0,726586945 Lpcat2b lysophosphatidylcholine acyltransferase 2B 1,14E-04 1,47E-04 1,291 -0,350 0,726586945 CYLC1 cylicin-1 3,67E-04 4,48E-04 1,220 -0,346 0,729059212 GRPEL1 grpE protein homolog 1, mitochondrial precursor 2,50E-04 1,99E-04 1,261 0,346 0,729464282 cation channel sperm-associated protein subunit delta TMEM146 precursor 2,57E-04 2,89E-04 1,127 -0,343 0,731427874 COPS7A COP9 signalosome complex subunit 7a isoform 2 1,21E-04 1,61E-04 1,331 -0,343 0,731672158 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA3 subunit 3 4,52E-04 5,99E-04 1,323 -0,343 0,731672158 Cct3 T-complex protein 1 subunit gamma 0,001321618 0,001423076 1,077 -0,339 0,734694723 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, Ndufs8 mitochondrial 0,001070616 9,78E-04 1,095 0,336 0,736720582 Sypl synaptophysin-like protein 1 isoform 1 7,93E-04 7,75E-04 1,023 0,334 0,738176693

Carkd ATP-dependent (S)-NAD(P)H-hydrate dehydratase isoform 1 3,49E-04 3,14E-04 1,112 0,334 0,738176693 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia CCDC135 coiled-coil domain-containing protein lobo homolog 2,20E-04 2,07E-04 1,064 0,331 0,740813182 1700011F03Rik testis-expressed sequence 37 protein 0,001214217 0,001006744 1,206 0,331 0,740813182 wbp2nl postacrosomal sheath WW domain-binding protein 8,00E-04 7,15E-04 1,120 0,331 0,740813182 NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, NDUFS7 mitochondrial precursor 0,001971037 0,002184117 1,108 -0,330 0,741254035 Gm5928 ubiquitin-40S ribosomal protein S27a precursor 0,003155673 0,003168195 1,004 -0,327 0,743666946 3-hydroxyisobutyrate dehydrogenase, mitochondrial hibadh precursor 0,002031992 0,001939088 1,048 0,325 0,745069703 Pfn3 profilin-3 0,0010318 0,00113044 1,096 -0,324 0,745615054 cct4 T-complex protein 1 subunit delta 9,10E-04 8,64E-04 1,054 0,323 0,746506564 - uncharacterized protein LOC73863 4,19E-04 3,40E-04 1,232 0,320 0,748831287 INSL6 insulin-like peptide INSL6 precursor 2,52E-04 2,34E-04 1,076 0,320 0,748831287 Dnaja2 dnaJ homolog subfamily A member 2 1,17E-04 1,01E-04 1,153 0,320 0,748831287 NME7 nucleoside diphosphate kinase 7 isoform 1 5,85E-04 5,71E-04 1,024 0,318 0,750655816 single-stranded DNA-binding protein, mitochondrial isoform SSBP1 1 6,62E-04 6,04E-04 1,096 0,311 0,755595506 4933411K16Rik uncharacterized protein C10orf62 homolog 9,05E-04 8,77E-04 1,031 0,310 0,756306401 Car4 carbonic anhydrase 4 precursor 0,001913745 0,001791858 1,068 0,308 0,758170795 Tssk1 testis-specific serine/threonine-protein kinase 1 7,78E-04 7,30E-04 1,066 0,308 0,758395427 coiled-coil-helix-coiled-coil-helix domain-containing protein CHCHD6 6, mitochondrial isoform 1 0,001403999 0,00148473 1,058 0,306 0,759289651 RSPH9 radial spoke head protein 9 homolog 0,002076994 0,002326372 1,120 -0,304 0,761292182 Psma6 proteasome subunit alpha type-6 7,84E-04 7,28E-04 1,076 0,299 0,765086501 CCT2 T-complex protein 1 subunit beta 9,88E-04 0,001026517 1,039 -0,299 0,76531521

HIBCH 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial precursor 3,63E-04 3,99E-04 1,098 -0,295 0,767694748 PRSS44 serine protease 44 precursor 1,76E-04 1,61E-04 1,092 0,292 0,770071937 MORN2 MORN repeat-containing protein 2 4,33E-04 4,02E-04 1,076 0,292 0,770071937 Lypd4 ly6/PLAUR domain-containing protein 4 precursor 0,002325515 0,002375705 1,022 -0,291 0,771432246 ACYP1 acylphosphatase-1 4,84E-04 5,42E-04 1,121 -0,288 0,773081263 Dusp21 dual specificity phosphatase 21 8,71E-04 9,74E-04 1,118 -0,288 0,773081263 1700029J07Rik UPF0602 protein C4orf47 homolog 2,18E-04 2,61E-04 1,197 -0,288 0,773081263 slc16a7 monocarboxylate transporter 2 4,95E-04 5,38E-04 1,088 -0,288 0,773081263 Lrrc51 leucine-rich repeat-containing protein 51 isoform 1 6,06E-04 5,85E-04 1,036 0,287 0,774104013 Dnahc10 dynein heavy chain 10, axonemal 6,62E-05 6,92E-05 1,045 -0,272 0,785459932 LRRC34 leucine-rich repeat-containing protein 34 2,42E-04 3,04E-04 1,257 -0,270 0,787057349 SCAMP2 secretory carrier-associated membrane protein 2 0,002760458 0,002927815 1,061 -0,265 0,791035981 HSD17B10 3-hydroxyacyl-CoA dehydrogenase type-2 7,00E-04 7,91E-04 1,129 -0,265 0,791035981 Aldoa2 fructose-bisphosphate aldolase A isoform 2 0,00128219 0,001438588 1,122 -0,262 0,793429981

Abca14 ATP-binding cassette, sub-family A (ABC1), member 14 2,62E-05 2,13E-05 1,233 0,261 0,793767653 sodium/potassium-transporting ATPase subunit alpha-1 ATP1A1 precursor 3,63E-05 3,15E-05 1,153 0,261 0,793767653 Tmem89 transmembrane protein 89 precursor 4,04E-04 3,19E-04 1,267 0,261 0,793767653 FDX1L adrenodoxin-like protein, mitochondrial precursor 1,80E-04 1,50E-04 1,201 0,261 0,793767653 GCLM glutamate--cysteine ligase regulatory subunit 2,06E-04 1,78E-04 1,157 0,261 0,793767653 tk2 thymidine kinase 2, mitochondrial precursor 1,34E-04 1,09E-04 1,235 0,261 0,793767653 RAB28 ras-related protein Rab-28 1,16E-04 9,93E-05 1,169 0,261 0,793767653 - predicted gene, 17727 precursor 2,95E-04 3,01E-04 1,020 0,261 0,793767653 Gm16379 macrophage migration inhibitory factor 4,57E-04 4,02E-04 1,138 0,261 0,793767653 PREDICTED: cytochrome c oxidase subunit 7C, Cox7c mitochondrial-like 4,92E-04 4,47E-04 1,102 0,261 0,793767653 LYZL6 lysozyme-like protein 6 precursor 0,001642407 0,001504172 1,092 0,261 0,793866003 TMEM30A cell cycle control protein 50A 1,56E-04 2,05E-04 1,317 -0,260 0,79512221 IQCD IQ domain-containing protein D 7,24E-05 9,03E-05 1,248 -0,260 0,79512221 Efcab9 EF-hand calcium-binding domain-containing protein 9 2,47E-04 2,95E-04 1,196 -0,260 0,79512221 HSP90AB1 heat shock protein HSP 90-beta 5,53E-05 6,49E-05 1,174 -0,260 0,79512221 hprT hypoxanthine-guanine phosphoribosyltransferase 1,63E-04 1,83E-04 1,128 -0,260 0,79512221

Uqcrc1 cytochrome b-c1 complex subunit 1, mitochondrial precursor 0,004716734 0,00527534 1,118 0,259 0,795971113 dolichyl-diphosphooligosaccharide--protein DAD1 glycosyltransferase subunit DAD1 2,10E-04 2,55E-04 1,210 -0,242 0,808421983 sar1a GTP-binding protein SAR1a 8,86E-05 1,10E-04 1,246 -0,242 0,808421983 GDE1 glycerophosphodiester phosphodiesterase 1 6,17E-05 7,47E-05 1,210 -0,242 0,808421983 YWHAB 14-3-3 protein beta/alpha 6,81E-05 8,25E-05 1,212 -0,242 0,808421983 Mcpt3 mast cell protease 4 precursor 8,88E-05 1,11E-04 1,247 -0,242 0,808421983 Trappc5 trafficking protein particle complex subunit 5 7,38E-05 9,44E-05 1,278 -0,242 0,808421983 COASY bifunctional coenzyme A synthase precursor 5,06E-05 6,43E-05 1,272 -0,242 0,808421983 4933402N03Rik uncharacterized protein LOC233918 4,52E-05 5,59E-05 1,238 -0,242 0,808421983

Cdc42 cell division control protein 42 homolog isoform 1 precursor 8,29E-05 1,06E-04 1,284 -0,242 0,808421983 ak7 adenylate kinase 7 7,94E-04 7,94E-04 1,000 -0,240 0,809943857 AA467197 normal mucosa of esophagus-specific gene 1 protein 0,004325182 0,004605966 1,065 0,239 0,811209056 SPACA5 sperm acrosome-associated protein 5 precursor 5,62E-04 6,36E-04 1,131 -0,234 0,815035839 rpiA ribose-5-phosphate isomerase 2,27E-04 2,53E-04 1,113 -0,234 0,815035839 Samm50 sorting and assembly machinery component 50 homolog 7,55E-04 8,06E-04 1,068 -0,233 0,816015989 Thnsl1 threonine synthase-like 1 isoform a 5,47E-04 5,32E-04 1,028 0,229 0,818512945 Crisp2 cysteine-rich secretory protein 2 precursor 1,40E-04 1,15E-04 1,220 0,226 0,820894066 dolichyl-diphosphooligosaccharide--protein Rpn2 glycosyltransferase subunit 2 precursor 5,91E-05 5,01E-05 1,179 0,226 0,820894066 ptrh2 peptidyl-tRNA hydrolase 2, mitochondrial isoform b 1,30E-04 1,11E-04 1,180 0,226 0,820894066 lyrm1 LYR motif-containing protein 1 1,97E-04 1,69E-04 1,168 0,226 0,820894066 succinate dehydrogenase [ubiquinone] iron-sulfur subunit, LOC638710 mitochondrial precursor 0,001428904 0,001428569 1,000 0,223 0,823275846 RAB4B ras-related protein Rab-4B 4,80E-04 4,85E-04 1,011 0,220 0,826009988 Gm614 uncharacterized protein CXorf65 homolog 6,11E-04 5,73E-04 1,066 0,220 0,826009988 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia gtl3 UPF0468 protein C16orf80 homolog 0,001439794 0,001469589 1,021 -0,219 0,826575775 PTCHD3 patched domain-containing protein 3 8,90E-04 8,93E-04 1,003 -0,219 0,826589835 Rab14 ras-related protein Rab-14 0,002840961 0,002781134 1,022 0,219 0,826619877 serine/threonine-protein phosphatase 2A 65 kDa regulatory PPP2R1A subunit A alpha isoform 1,45E-04 1,32E-04 1,100 0,204 0,838035074 1110032A03Rik UPF0686 protein C11orf1 homolog 6,55E-04 6,59E-04 1,006 0,204 0,838035074 Gm12231 ATP synthase subunit b, mitochondrial precursor 0,001680577 0,001597778 1,052 0,202 0,839560495 CCT8L1 chaperonin containing TCP1, subunit 8 (theta)-like 1 3,95E-04 4,19E-04 1,060 -0,199 0,842522989 ME2 NAD-dependent malic enzyme, mitochondrial precursor 4,32E-04 4,31E-04 1,003 0,197 0,843542935

TPPP2 tubulin polymerization-promoting protein family member 2 5,80E-04 6,16E-04 1,061 -0,192 0,847395591 NUP54 nuclear pore complex protein Nup54 1,08E-04 1,23E-04 1,143 -0,191 0,84852559 HIST2H3B histone H2A type 2-A 3,95E-04 4,78E-04 1,212 -0,191 0,84852559 ACTB actin, cytoplasmic 1 0,001250808 0,001476232 1,180 -0,190 0,849451359 Gm7172 tubulin alpha-1A chain 8,72E-04 8,76E-04 1,005 -0,188 0,850727924 NT5C1B cytosolic 5-nucleotidase 1B 0,004633137 0,004628911 1,001 0,188 0,850811008 1700009P17Rik UPF0740 protein C1orf192 homolog 7,93E-04 9,11E-04 1,148 -0,185 0,853239216 PPIL6 peptidyl-prolyl cis-trans isomerase-like 6 3,81E-04 4,16E-04 1,091 -0,185 0,853239216 ppp2r4 serine/threonine-protein phosphatase 2A activator 2,50E-04 2,69E-04 1,074 -0,185 0,853239216 p4hb protein disulfide-isomerase precursor 1,36E-04 1,45E-04 1,065 -0,185 0,853239216 RPL18 60S ribosomal protein L18 7,94E-05 7,13E-05 1,114 0,185 0,853347436 SERPINE2 glia-derived nexin precursor 4,93E-05 4,02E-05 1,225 0,185 0,853347436 atp6v0d1 V-type proton ATPase subunit d 1 5,06E-05 4,17E-05 1,213 0,185 0,853347436 KRT6A keratin, type II cytoskeletal 6A 3,65E-05 3,47E-05 1,054 0,185 0,853347436 RAB22A ras-related protein Rab-22A 6,36E-05 5,70E-05 1,117 0,185 0,853347436 MYCBPAP MYCBP-associated protein 2,74E-05 2,32E-05 1,184 0,185 0,853347436 Atp1b3 sodium/potassium-transporting ATPase subunit beta-3 0,001354716 0,001401258 1,034 -0,182 0,855685816 Nit1 nitrilase homolog 1 isoform 2 8,01E-04 7,64E-04 1,048 0,176 0,859934752 NADH-ubiquinone oxidoreductase 75 kDa subunit, NDUFS1 mitochondrial precursor 0,002302508 0,002458881 1,068 -0,176 0,860473162 dtymk thymidylate kinase isoform 1 2,76E-04 2,67E-04 1,033 0,172 0,863057013 FAM78A protein FAM78A 3,88E-04 3,50E-04 1,108 0,172 0,863057013 atp5s ATP synthase subunit s, mitochondrial precursor 7,75E-04 7,10E-04 1,091 0,171 0,864295229 NCSTN nicastrin precursor 3,42E-04 3,40E-04 1,004 0,171 0,864295229 DYDC1 DPY30 domain-containing protein 1 8,81E-04 8,73E-04 1,010 0,171 0,864295229 PSMB4 proteasome subunit beta type-4 precursor 8,63E-04 9,17E-04 1,062 -0,169 0,865662008 Adam4 a disintegrin and metallopeptidase domain 4 precursor 2,34E-04 2,61E-04 1,115 -0,160 0,873100875 PSMC5 26S protease regulatory subunit 8 3,06E-04 3,25E-04 1,059 -0,160 0,873100875 aldoa1a aldolase 1 A retrogene 1 5,38E-04 6,18E-04 1,148 -0,150 0,880653789 monofunctional C1-tetrahydrofolate synthase, mitochondrial MTHFD1L precursor 1,25E-04 1,36E-04 1,088 -0,150 0,880739833 TSNAXIP1 translin-associated factor X-interacting protein 1 1,66E-04 1,68E-04 1,016 -0,150 0,880739833 RSPH6A radial spoke head protein 6 homolog A isoform 1 0,001399858 0,001295658 1,080 0,148 0,882045122 Ak2 adenylate kinase 2, mitochondrial isoform b 0,00192146 0,001984876 1,033 0,144 0,885564888 slc25a10 mitochondrial dicarboxylate carrier 8,60E-04 9,06E-04 1,054 0,143 0,886203578 1700028P14Rik uncharacterized protein C9orf135 homolog 2,68E-04 3,09E-04 1,155 -0,140 0,888295453 Gm9234 peptidyl-prolyl cis-trans isomerase A 4,30E-04 4,57E-04 1,063 -0,140 0,888295453 FANK1 fibronectin type 3 and ankyrin repeat domains 1 protein 2,80E-04 2,72E-04 1,028 0,140 0,889026445 Gm12906 mitochondrial import receptor subunit TOM22 homolog 0,001089806 0,001093137 1,003 0,140 0,889026445 FAM154A uncharacterized protein LOC75811 9,52E-04 9,91E-04 1,041 0,138 0,890240533 tekt3 tektin-3 0,002412476 0,002396197 1,007 0,138 0,890327659 4921517D21Rik uncharacterized protein LOC67722 7,88E-05 7,56E-05 1,042 0,138 0,890627004 YWHAE 14-3-3 protein epsilon 2,09E-04 1,90E-04 1,098 0,138 0,890627004 2410015M20Rik protein QIL1 precursor 6,53E-04 7,14E-04 1,094 0,138 0,890627004 SPINT4 kunitz-type protease inhibitor 4 precursor 4,02E-04 4,01E-04 1,004 0,138 0,890627004 1700007G11Rik uncharacterized protein C4orf22 homolog 3,46E-04 3,32E-04 1,041 0,138 0,890627004 Spag8 sperm-associated antigen 8 8,71E-05 1,08E-04 1,237 -0,135 0,892570241 Krt16 keratin, type I cytoskeletal 16 5,93E-05 6,14E-05 1,037 -0,135 0,892570241 LOC100048051 multidrug and toxin extrusion protein 2 9,60E-05 1,06E-04 1,099 -0,135 0,892570241 hydroxyacyl-coenzyme A dehydrogenase, mitochondrial hadh precursor 9,47E-05 1,01E-04 1,064 -0,135 0,892570241 ube4a ubiquitin conjugation factor E4 A 3,91E-05 4,48E-05 1,146 -0,135 0,892570241 iron-sulfur cluster assembly 2 homolog, mitochondrial iscA2 precursor 1,05E-04 1,29E-04 1,231 -0,135 0,892570241 NIPSNAP1 protein NipSnap homolog 1 8,65E-05 1,01E-04 1,172 -0,135 0,892570241 ALDH1A2 retinal dehydrogenase 2 5,11E-05 5,92E-05 1,157 -0,135 0,892570241 PSMD8 26S proteasome non-ATPase regulatory subunit 8 1,84E-04 2,11E-04 1,146 -0,132 0,894627663 DNAJB3 dnaJ homolog subfamily B member 3 2,29E-04 2,63E-04 1,149 -0,132 0,894627663 Ubtd2 ubiquitin domain-containing protein 2 2,92E-04 3,11E-04 1,062 -0,132 0,894627663 FKBP1A peptidyl-prolyl cis-trans isomerase FKBP1A 5,08E-04 5,37E-04 1,057 -0,132 0,894627663 Ribc2 RIB43A-like with coiled-coils protein 2 3,84E-04 4,24E-04 1,105 -0,129 0,897696707 Iqcg IQ domain-containing protein G 4,44E-04 4,58E-04 1,032 -0,129 0,897696707 - PREDICTED: WD repeat-containing protein 66 2,18E-04 2,25E-04 1,032 -0,127 0,899059546 RAB11B ras-related protein Rab-11B 0,001850543 0,001802474 1,027 0,123 0,901969574 CLMN calmin isoform a 1,56E-04 1,65E-04 1,059 -0,115 0,908450934 Arrdc5 arrestin domain-containing protein 5 3,83E-04 4,11E-04 1,074 -0,115 0,908450934 PSMB3 proteasome subunit beta type-3 7,87E-04 8,35E-04 1,062 -0,115 0,908450934

ALDH9A1 4-trimethylaminobutyraldehyde dehydrogenase precursor 3,81E-04 3,97E-04 1,041 -0,114 0,909551071 Acad10 acyl-CoA dehydrogenase family member 10 1,05E-04 1,02E-04 1,035 0,109 0,91281 Zmynd12 zinc finger, MYND domain containing 12 5,68E-04 5,17E-04 1,100 0,109 0,912858569 ATP8B3 probable phospholipid-transporting ATPase IK 2,98E-04 3,16E-04 1,063 -0,107 0,914789775 4930511I11Rik armadillo repeat-containing protein 12 0,001064425 0,001218772 1,145 -0,102 0,918476445 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA5 subunit 5 0,00148638 0,001397364 1,064 -0,099 0,921266377 Efcab2 EF-hand calcium-binding domain-containing protein 2 4,34E-04 4,24E-04 1,024 0,099 0,921408565 Valor de abundancia APEX Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spretus Cociente APEX Z-score p presenta mayor abundancia LOC100045999 PREDICTED: GTP-binding nuclear protein Ran-like 2,48E-04 2,48E-04 1,001 0,099 0,921408565 Gykl1 glycerol kinase-like 1 0,002102319 0,002044224 1,028 -0,096 0,923135594 APOA1BP NAD(P)H-hydrate epimerase precursor 6,23E-04 5,83E-04 1,068 0,095 0,924500602 ftmt ferritin, mitochondrial precursor 6,06E-04 6,13E-04 1,012 0,095 0,924500602 SLC25A20 mitochondrial carnitine/acylcarnitine carrier protein 9,53E-04 9,51E-04 1,001 -0,093 0,925535121 Psma3 proteasome subunit alpha type-3 6,92E-04 7,26E-04 1,048 -0,087 0,930392037 Tubb2a-ps2 tubulin beta-2B chain 3,14E-04 3,13E-04 1,004 0,085 0,931901992

TTC19 tetratricopeptide repeat protein 19, mitochondrial isoform 1 2,03E-04 2,12E-04 1,046 -0,081 0,935199593 CCT6B T-complex protein 1 subunit zeta-2 1,20E-04 1,29E-04 1,070 -0,081 0,935199593 uncharacterized protein C2orf47 homolog, mitochondrial 9430016H08Rik precursor 3,11E-04 3,24E-04 1,042 0,077 0,93832828 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, Gm12251 mitochondrial precursor 0,002238367 0,002455362 1,097 -0,068 0,945802549 BCS1L mitochondrial chaperone BCS1 3,61E-04 3,53E-04 1,024 0,067 0,946662386 VDAC1 voltage-dependent anion-selective channel protein 1 3,91E-04 4,02E-04 1,028 0,067 0,946662386 LdhA L-lactate dehydrogenase A chain isoform 2 0,001834479 0,001959904 1,068 -0,065 0,947898924 BANF2 barrier-to-autointegration factor-like protein 2,23E-04 2,38E-04 1,070 -0,057 0,954156028 C4bp C4b-binding protein precursor 8,55E-05 9,47E-05 1,107 -0,057 0,954156028 a disintegrin and metalloprotease domain 3 (cyritestin) Adam3 precursor 5,31E-05 5,53E-05 1,042 -0,057 0,954156028 iron-sulfur cluster assembly 1 homolog, mitochondrial iscA1 precursor 1,89E-04 2,06E-04 1,089 -0,057 0,954156028 USP5 ubiquitin carboxyl-terminal hydrolase 5 4,29E-05 4,53E-05 1,058 -0,057 0,954156028 Gm14316 serine protease inhibitor, Kunitz type 5 precursor 2,88E-04 3,40E-04 1,179 -0,057 0,954156028 SPINK2 serine protease inhibitor Kazal-type 2 precursor 4,24E-04 4,01E-04 1,059 -0,057 0,954156028 isyna1 inositol-3-phosphate synthase 1 1,37E-04 1,43E-04 1,041 0,055 0,956372604 STARD6 stAR-related lipid transfer protein 6 2,15E-04 1,99E-04 1,081 0,055 0,956372604 delta-1-pyrroline-5-carboxylate dehydrogenase, ALDH4A1 mitochondrial precursor 9,78E-05 9,99E-05 1,022 0,055 0,956372604 ATP8A2 probable phospholipid-transporting ATPase IB 5,22E-05 4,99E-05 1,048 0,055 0,956372604 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA1 subunit 1 5,93E-04 5,95E-04 1,004 0,055 0,956372604 LYZL1 lysozyme-like protein 1 precursor 4,52E-04 4,68E-04 1,036 0,055 0,956372604 PITRM1 presequence protease, mitochondrial precursor 3,34E-04 3,43E-04 1,026 0,051 0,959539032 TUBB2C tubulin beta-4B chain 0,003313337 0,003332613 1,006 0,050 0,960182755 bsg basigin isoform 2 precursor 0,003476643 0,00358995 1,033 -0,047 0,96272795 PREDICTED: mitochondrial pyruvate carrier-like protein- Gm13570 like 0,001739523 0,001715179 1,014 -0,043 0,96561775 LOC631229 ADP/ATP translocase 2 2,41E-04 2,63E-04 1,089 0,043 0,965865975 cul3 cullin-3 3,99E-04 4,26E-04 1,067 -0,042 0,966297931 acot9 acyl-coenzyme A thioesterase 9, mitochondrial 0,002670385 0,002592449 1,030 -0,040 0,968061259 ATP5J2 ATP synthase subunit f, mitochondrial 0,001237756 0,001238591 1,001 0,038 0,970060228

SATL1 spermidine/spermine N(1)-acetyltransferase-like protein 1 1,73E-04 1,85E-04 1,070 -0,036 0,971444691 Odf3l1 outer dense fiber protein 3-like protein 1 2,89E-04 3,26E-04 1,128 -0,036 0,971444691 mitochondrial translocator assembly and mainte-ce protein 1500001M20Rik 41 homolog precursor 2,94E-04 3,44E-04 1,172 -0,036 0,971444691 BC089491 selenoprotein V 2,26E-04 2,58E-04 1,139 -0,036 0,971444691 Uba1 ubiquitin-like modifier-activating enzyme 1 isoform 2 2,12E-04 2,10E-04 1,013 0,035 0,972424483 Gm11273 cytochrome c oxidase subunit 5B, mitochondrial 0,001782416 0,001792688 1,006 -0,033 0,973569134 CAND1 cullin-associated NEDD8-dissociated protein 1 2,89E-04 2,98E-04 1,030 -0,030 0,976436848 Sept4 septin-4 6,43E-04 7,23E-04 1,125 -0,030 0,976436848 RAB2A ras-related protein Rab-2A 0,008902561 0,008857603 1,005 -0,029 0,976802892

COX5A cytochrome c oxidase subunit 5A, mitochondrial precursor 0,002934696 0,003235071 1,102 -0,022 0,982772508 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex ndufa6 subunit 6 0,001120881 0,001093545 1,025 -0,021 0,983340634 Hist1h4c histone H4 8,77E-04 9,15E-04 1,043 0,006 0,994837547 Slc22a21 solute carrier family 22 member 21 4,61E-04 4,58E-04 1,007 0,006 0,994837547 WARS2 tryptophan--tRNA ligase, mitochondrial precursor 1,14E-04 1,15E-04 1,002 0,004 0,997019772 NFU1 iron-sulfur cluster scaffold homolog, mitochondrial Gm7859 isoform 2 precursor 1,98E-04 2,22E-04 1,121 0,004 0,997019772 TXNDC3 thioredoxin domain-containing protein 3 isoform 1 0,001099758 0,001087846 1,011 -0,002 0,998399105 Tabla suplementaria 13. Análisis comparativos entre Mus musculus y Mus spicilegus . Las proteínas con diferencias significativas son indicadas en negrita.

Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia AKAP4 A-kinase anchor protein 4 isoform b 0,005859698 0,002431018 2,410 14,813 0 Mus musculus LOC100046120 clusterin precursor 0,003772178 8,33E-04 4,530 8,957 0 Mus musculus

Gpd2 glycerol-3-phosphate dehydrogenase, mitochondrial precursor 0,00268629 0,001190297 2,257 8,698 0 Mus musculus HADHA trifunctional enzyme subunit alpha, mitochondrial precursor 0,001883598 6,70E-04 2,810 7,281 3,31E-13 Mus musculus FSCB fibrous sheath CABYR-binding protein 0,003323024 0,001454292 2,285 7,050 1,79E-12 Mus musculus Gm13910 trifunctional enzyme subunit beta, mitochondrial precursor 0,002572318 7,62E-04 3,375 6,969 3,20E-12 Mus musculus Hbb hemoglobin, beta adult s chain 0,004816249 0,001704711 2,825 6,589 4,42E-11 Mus musculus Irgc1 interferon-inducible GTPase 5 0,003177998 0,001435365 2,214 6,581 4,69E-11 Mus musculus Pacrg parkin coregulated gene protein homolog 0,002466453 4,93E-04 5,004 6,488 8,71E-11 Mus musculus Gm7172 tubulin alpha-1A chain 8,72E-04 1,80E-04 4,838 5,849 4,94E-09 Mus musculus phospholipid hydroperoxide glutathione peroxidase, nuclear isoform Gpx4 1 0,003446861 0,00149395 2,307 5,807 6,38E-09 Mus musculus PFKM 6-phosphofructokinase, muscle type 0,001031216 3,43E-04 3,003 5,623 1,88E-08 Mus musculus ACAT1 acetyl-CoA acetyltransferase, mitochondrial precursor 0,001886715 6,82E-04 2,766 5,251 1,51E-07 Mus musculus

Uqcrc1 cytochrome b-c1 complex subunit 1, mitochondrial precursor 0,004716734 0,002899343 1,627 5,233 1,67E-07 Mus musculus GSTM5 glutathione S-transferase Mu 5 0,003974018 0,002329259 1,706 4,876 1,08E-06 Mus musculus NT5C1B cytosolic 5-nucleotidase 1B 0,004633137 0,002939427 1,576 4,731 2,24E-06 Mus musculus tekt3 tektin-3 0,002412476 0,001317636 1,831 4,601 4,20E-06 Mus musculus Rsph1 radial spoke head 1 homolog 0,002686207 0,001102787 2,436 4,356 1,33E-05 Mus musculus

BDH1 D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor 0,001725233 7,86E-04 2,196 4,332 1,48E-05 Mus musculus TEKT2 tektin-2 0,001748578 9,09E-04 1,923 4,299 1,71E-05 Mus musculus Odf2 outer dense fiber protein 2 isoform e 0,00435 0,003166166 1,374 4,295 1,75E-05 Mus musculus very long-chain specific acyl-CoA dehydrogenase, mitochondrial ACADVL precursor 2,65E-04 3,15E-05 8,412 4,246 2,18E-05 Mus musculus BSPRY B box and SPRY domain-containing protein 0,001360709 5,86E-04 2,322 4,226 2,38E-05 Mus musculus spata18 mitochondria-eating protein 0,002399523 0,001297617 1,849 4,155 3,26E-05 Mus musculus Rsph3b radial spoke head protein 3 homolog B 0,001725308 7,99E-04 2,161 4,144 3,41E-05 Mus musculus Gm1096 PREDICTED: CD177 antigen isoform 2 0,001153694 2,14E-04 5,393 4,120 3,79E-05 Mus musculus SCAMP1 secretory carrier-associated membrane protein 1 9,61E-04 0,002679324 2,789 -4,096 4,20E-05 Mus spicilegus MUC5B mucin 5, subtype B, tracheobronchial precursor 3,54E-05 1,40E-04 3,963 -4,168 3,07E-05 Mus spicilegus monofunctional C1-tetrahydrofolate synthase, mitochondrial MTHFD1L precursor 1,25E-04 3,91E-04 3,128 -4,209 2,56E-05 Mus spicilegus FCGBP Fc fragment of IgG binding protein-like precursor 4,59E-04 7,72E-04 1,685 -4,224 2,40E-05 Mus spicilegus acsbg2 long-chain-fatty-acid--CoA ligase ACSBG2 6,14E-04 0,001302264 2,122 -4,411 1,03E-05 Mus spicilegus 4933400C05Rik spermatogenesis-associated protein 32 2,59E-04 0,0011809 4,562 -4,449 8,64E-06 Mus spicilegus zpbp1 zona pellucida-binding protein 1 isoform 2 0,004449361 0,00710688 1,597 -4,481 7,42E-06 Mus spicilegus Gm884 uncharacterized protein LOC380730 8,07E-04 0,001427589 1,769 -4,510 6,49E-06 Mus spicilegus ZPBP2 zona pellucida-binding protein 2 isoform 1 precursor 0,001085162 0,002693968 2,483 -4,743 2,10E-06 Mus spicilegus Zp3r zona pellucida sperm-binding protein 3 receptor precursor 0,001208789 0,002642884 2,186 -4,845 1,27E-06 Mus spicilegus PPEF1 serine/threonine-protein phosphatase with EF-hands 1 9,08E-05 5,48E-04 6,029 -4,846 1,26E-06 Mus spicilegus Gm4787 a disintegrin and metalloprotease domain 4b precursor 3,79E-05 4,08E-04 10,752 -4,866 1,14E-06 Mus spicilegus PREDICTED: LOW QUALITY PROTEIN: dynein heavy chain 3, dnhc3 axonemal 4,01E-05 1,34E-04 3,346 -4,965 6,87E-07 Mus spicilegus CD109 CD109 antigen precursor 2,36E-04 6,93E-04 2,939 -5,111 3,20E-07 Mus spicilegus 4930519F16Rik uncharacterized protein LOC75106 precursor 2,99E-04 0,002122381 7,109 -5,223 1,76E-07 Mus spicilegus VPS13A vacuolar protein sorting-associated protein 13A 6,29E-05 2,41E-04 3,831 -5,329 9,89E-08 Mus spicilegus MAN2B2 epididymis-specific alpha-mannosidase precursor 5,29E-04 0,001458717 2,759 -5,545 2,93E-08 Mus spicilegus Hspa4l heat shock 70 kDa protein 4L 2,11E-04 7,19E-04 3,399 -5,705 1,16E-08 Mus spicilegus MECR trans-2-enoyl-CoA reductase, mitochondrial precursor 1,72E-04 0,001344568 7,827 -6,021 1,73E-09 Mus spicilegus Smrp1 spermatid-specific manchette-related protein 1 isoform 3 1,21E-04 0,001145712 9,496 -6,340 2,29E-10 Mus spicilegus zan zonadhesin precursor 4,37E-04 0,00110264 2,522 -11,916 9,75E-33 Mus spicilegus SPATC1 speriolin 0,001991203 8,83E-04 2,255 4,011 6,03E-05 ODF1 outer dense fiber protein 1 0,002856277 0,001646346 1,735 4,005 6,19E-05 PLB1 phospholipase B1, membrane-associated isoform 1 precursor 5,26E-04 2,64E-04 1,993 3,978 6,96E-05 GAPDHS glyceraldehyde-3-phosphate dehydrogenase, testis-specific 0,006845997 0,005258094 1,302 3,959 7,53E-05 TPI1 triosephosphate isomerase 0,002633418 0,001564426 1,683 3,825 1,31E-04 ACO2 aconitate hydratase, mitochondrial precursor 0,005409188 0,004379714 1,235 3,769 1,64E-04 KRT19 keratin, type I cytoskeletal 19 3,62E-04 7,33E-05 4,946 3,739 1,84E-04 OXSM 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial precursor 7,90E-04 2,47E-04 3,194 3,671 2,42E-04 Ccdc114 coiled-coil domain-containing protein 114 5,00E-04 1,63E-04 3,065 3,671 2,42E-04 oplaH 5-oxoprolinase 2,44E-04 5,54E-05 4,401 3,658 2,54E-04 PGP phosphoglycolate phosphatase 8,18E-04 2,64E-04 3,092 3,623 2,91E-04 Spna1 spectrin alpha chain, erythrocytic 1 1,22E-04 4,12E-05 2,955 3,610 3,06E-04 abcb8 ATP-binding cassette sub-family B member 8, mitochondrial precursor 2,48E-04 3,54E-05 7,026 3,595 3,24E-04 SQRDL sulfide:quinone oxidoreductase, mitochondrial 6,33E-04 2,40E-04 2,634 3,555 3,78E-04

Isoc2a isochorismatase domain-containing protein 2A, mitochondrial precursor 0,003428162 0,001321028 2,595 3,552 3,82E-04 chdh choline dehydrogenase, mitochondrial 7,24E-04 3,16E-04 2,292 3,533 4,10E-04 TEKT4 tektin-4 9,13E-04 4,55E-04 2,009 3,504 4,58E-04 SLC2A3 solute carrier family 2, facilitated glucose transporter member 3 0,00542572 0,003357845 1,616 3,493 4,77E-04 dld dihydrolipoyl dehydrogenase, mitochondrial precursor 0,004114163 0,002677128 1,537 3,468 5,24E-04 CATSPERB cation channel sperm-associated protein subunit beta precursor 4,07E-04 1,63E-04 2,496 3,460 5,41E-04 ptpmt1 phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1 9,62E-04 3,30E-04 2,919 3,446 5,68E-04 TEKT5 tektin-5 0,001667094 9,40E-04 1,774 3,434 5,95E-04 HK1 hexokinase-1 isoform HK1-sb 0,004577325 0,003929661 1,165 3,400 6,75E-04 Echs1 enoyl-CoA hydratase, mitochondrial precursor 6,49E-04 1,55E-04 4,177 3,368 7,58E-04 - PREDICTED: uncharacterized protein LOC77908 0,00324911 0,001119488 2,902 3,331 8,65E-04 COX4I1 cytochrome c oxidase subunit 4 isoform 1, mitochondrial precursor 0,008421194 0,005993724 1,405 3,326 8,80E-04 Slc25a21 mitochondrial 2-oxodicarboxylate carrier isoform 1 6,81E-04 2,15E-04 3,172 3,287 0,00101424 vars valine--tRNA ligase 1,88E-04 5,86E-05 3,204 3,235 0,00121832 SLC4A1 band 3 anion transport protein 0,001020103 4,80E-04 2,125 3,191 0,00141632 acot9 acyl-coenzyme A thioesterase 9, mitochondrial 0,002670385 0,001704036 1,567 3,183 0,00145839 Glipr1l3 GLI pathogenesis-related 1 like 1 precursor 0,001749175 7,73E-04 2,263 3,167 0,00153831 Wfdc16 WAP four-disulfide core domain 16 0,002312074 4,45E-04 5,193 3,153 0,00161451 Fam162a protein FAM162A 0,001396192 3,49E-04 4,006 3,090 0,00200153 CECR5 cat eye syndrome critical region protein 5 homolog precursor 8,18E-04 3,27E-04 2,500 3,039 0,00237362 Slc25a31 ADP/ATP translocase 4 0,003471802 0,002774344 1,251 3,022 0,00251383 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia CCIN calicin 0,001505647 9,07E-04 1,661 3,010 0,00261182 Smcp sperm mitochondrial-associated cysteine-rich protein 0,003612009 0,00168175 2,148 3,008 0,00262695 HK1 hexokinase-1 isoform HK1 0,004086136 0,003565829 1,146 3,003 0,00267488 LOC627816 serine/threonine-protein phosphatase PP1-gamma catalytic subunit 8,06E-04 3,22E-04 2,505 2,972 0,00295409 hspb9 heat shock protein beta-9 0,002248648 0,001212609 1,854 2,968 0,00299821 1700019O17Rik uncharacterized protein C2orf57 homolog 7,09E-04 2,08E-04 3,402 2,945 0,00322968 KRT10 keratin, type I cytoskeletal 10 0,001137871 5,47E-04 2,079 2,919 0,00351136 L2HGDH L-2-hydroxyglutarate dehydrogenase, mitochondrial precursor 0,001121286 6,61E-04 1,697 2,900 0,00373134 pyruvate dehydrogenase E1 component subunit alpha, somatic form, Pdha1 mitochondrial precursor 5,85E-04 2,52E-04 2,320 2,868 0,00412698 PMPCA mitochondrial-processing peptidase subunit alpha precursor 2,79E-04 6,57E-05 4,254 2,858 0,00425937 CCDC27 coiled-coil domain-containing protein 27 1,80E-04 4,16E-05 4,333 2,858 0,00425937 Gm16486 PREDICTED: uncharacterized protein KIAA1683 5,08E-04 3,08E-04 1,651 2,853 0,0043297 LOC100046792 uncharacterized protein C6orf163 homolog 4,13E-04 1,33E-04 3,113 2,852 0,00433783 CCT2 T-complex protein 1 subunit beta 9,88E-04 5,81E-04 1,701 2,800 0,00510955 lonp1 lon protease homolog, mitochondrial precursor 3,38E-04 1,47E-04 2,293 2,777 0,00548212 lmna prelamin-A/C isoform C 3,18E-04 1,23E-04 2,589 2,777 0,00549422 MPI mannose-6-phosphate isomerase 4,18E-04 1,14E-04 3,683 2,742 0,006111 NME4 nucleoside diphosphate kinase, mitochondrial precursor 0,00117722 3,80E-04 3,098 2,705 0,00683531 electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial Etfdh precursor 2,12E-04 5,16E-05 4,108 2,694 0,00705889 Pkm pyruvate kinase, muscle isoform M1 0,006395322 0,005653915 1,131 2,684 0,00727606 Gm2199 mitochondrial carrier homolog 2 0,00232098 0,001319906 1,758 2,661 0,00778347 LMNB1 lamin-B1 1,25E-04 2,05E-05 6,082 2,637 0,00835372 TTC29 tetratricopeptide repeat protein 29 1,86E-04 3,03E-05 6,147 2,637 0,00835372

CUZD1 CUB and zona pellucida-like domain-containing protein 1 precursor 9,61E-04 5,19E-04 1,852 2,583 0,00979648 Spnb1 spectrin beta chain, erythrocytic 1,46E-04 8,00E-05 1,828 2,583 0,00979648 dihydrolipoyllysine-residue acetyltransferase component of pyruvate dlaT dehydrogenase complex, mitochondrial 0,005802588 0,004652064 1,247 2,552 0,0107131 Fam166a protein FAM166A 0,004866493 0,003907952 1,245 2,539 0,01110226 CYLC1 cylicin-1 3,67E-04 1,87E-04 1,965 2,538 0,01114355 Gm5506 alpha-enolase 0,005055182 0,004099178 1,233 2,507 0,01216207 KRT2 keratin, type II cytoskeletal 2 epidermal 5,62E-04 3,00E-04 1,870 2,497 0,01251143 Gm12141 60 kDa heat shock protein, mitochondrial 0,003417246 0,002833138 1,206 2,483 0,01301973 CRISP1 cysteine-rich secretory protein 1 precursor 6,13E-04 2,01E-04 3,045 2,481 0,01310617 PLIN4 perilipin-4 4,61E-05 8,99E-06 5,128 2,445 0,01448316 DNAJC11 dnaJ homolog subfamily C member 11 5,74E-04 3,09E-04 1,860 2,422 0,01542101 Akr7a5 aflatoxin B1 aldehyde reductase member 2 0,0010769 5,31E-04 2,029 2,378 0,0173875 Tmem126a transmembrane protein 126A 8,36E-04 2,64E-04 3,171 2,344 0,01908427 4930404N11Rik uncharacterized protein C19orf71 homolog 0,001890413 0,001129616 1,674 2,322 0,02023155 THEG testicular haploid expressed gene protein isoform 1 0,002067497 0,001544145 1,339 2,306 0,02111227 tpp2 tripeptidyl-peptidase 2 5,27E-04 3,52E-04 1,497 2,302 0,02135884 LOC100047179 protein-glutamine gamma-glutamyltransferase 4 1,59E-04 3,94E-05 4,029 2,284 0,02235129 DNAJB8 dnaJ homolog subfamily B member 8 4,21E-04 1,07E-04 3,931 2,284 0,02235129 AUH methylglutaconyl-CoA hydratase, mitochondrial precursor 1,94E-04 3,79E-05 5,117 2,240 0,02506748 GLUL glutamine synthetase 0,001114206 6,48E-04 1,718 2,238 0,02523963 FABP9 fatty acid-binding protein 9 0,004450144 0,004057311 1,097 2,232 0,02561739 cbr4 carbonyl reductase family member 4 0,001201316 6,66E-04 1,803 2,224 0,0261274 COX2 cytochrome c oxidase subunit II [Mus musculus castaneus] 0,00974733 0,008015447 1,216 2,180 0,02922293 tekt1 tektin-1 0,001027897 6,57E-04 1,564 2,175 0,02964633 4930511I11Rik armadillo repeat-containing protein 12 0,001064425 5,55E-04 1,917 2,163 0,03054562 CCDC40 coiled-coil domain-containing protein 40 5,05E-04 3,26E-04 1,549 2,161 0,03066667 1700065I17Rik uncharacterized protein C5orf48 homolog 0,001338791 5,36E-04 2,497 2,160 0,03078586 PPM1A protein phosphatase 1A 3,61E-04 1,49E-04 2,414 2,160 0,03078586 Ppp1r7 protein phosphatase 1 regulatory subunit 7 2,50E-04 7,86E-05 3,178 2,156 0,03107262 CCDC38 coiled-coil domain-containing protein 38 1,62E-04 4,94E-05 3,286 2,156 0,03107262 Cst12 cystatin-12 4,92E-04 1,33E-04 3,698 2,082 0,0373172 vdac2 voltage-dependent anion-selective channel protein 2 0,00250379 0,001860444 1,346 2,046 0,04072317 lipe hormone-sensitive lipase isoform 1 5,38E-04 3,36E-04 1,602 1,998 0,04567306 Gm8566 superoxide dismutase [Cu-Zn] 0,001518428 8,46E-04 1,794 1,972 0,04862017

Catsperg2 cation channel sperm-associated protein subunit gamma 2 precursor 2,84E-04 1,54E-04 1,843 1,932 0,05330968 1700006E09Rik uncharacterized protein C17orf105 homolog 0,001034708 6,24E-04 1,657 1,932 0,05330968

SLC25A3 PREDICTED: phosphate carrier protein, mitochondrial-like isoform 1 0,001129638 7,82E-04 1,444 1,913 0,05579583 pyruvate dehydrogenase E1 component subunit alpha, testis-specific pdhA2 form, mitochondrial precursor 0,007585223 0,006577202 1,153 1,903 0,057066 SPATA20 spermatogenesis-associated protein 20 2,67E-04 1,45E-04 1,841 1,900 0,05743659 Gm15785 dnaJ homolog subfamily B member 6 isoform a 0,001233989 8,92E-04 1,383 1,883 0,05964161 Sept10 septin-10 isoform 1 3,74E-04 1,74E-04 2,158 1,881 0,0600057 pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial 4930402E16Rik precursor 2,16E-04 1,11E-04 1,952 1,881 0,0600057 4921530D09Rik uncharacterized coiled-coil domain-containing protein KIAA1984 7,91E-04 5,40E-04 1,465 1,872 0,06120129 Ubxn11 UBX domain-containing protein 11 5,23E-04 2,90E-04 1,804 1,871 0,0613559 Prss46 serine protease 46 0,002958748 0,002252203 1,314 1,869 0,0616 Cops8 COP9 signalosome complex subunit 8 5,41E-04 1,74E-04 3,113 1,867 0,06186897 Gm6242 myosin regulatory light chain 12B 3,83E-04 1,23E-04 3,120 1,867 0,06186897 CAR1 carbonic anhydrase 1 7,87E-04 4,08E-04 1,929 1,833 0,06680791 Iqcg IQ domain-containing protein G 4,44E-04 2,20E-04 2,014 1,833 0,06680791 ank1 ankyrin-1 isoform 2 7,92E-05 3,63E-05 2,181 1,824 0,06820861 pgam5 serine/threonine-protein phosphatase PGAM5, mitochondrial isoform 2 7,75E-04 4,29E-04 1,808 1,818 0,06910567 Gm13570 brain protein 44-like protein 2 0,003514886 0,001994798 1,762 1,808 0,07065361 NIPSNAP3A nipsnap homolog 3A 0,003642805 0,002472802 1,473 1,776 0,07580387 Hspa2 heat shock-related 70 kDa protein 2 0,001503172 0,001203049 1,249 1,768 0,07705514 ccdc65 coiled-coil domain-containing protein 65 3,38E-04 1,89E-04 1,790 1,751 0,07991186 COPS4 COP9 signalosome complex subunit 4 2,02E-04 7,47E-05 2,703 1,749 0,08022467

Cabyr calcium-binding tyrosine phosphorylation-regulated protein isoform a 9,39E-04 5,49E-04 1,712 1,738 0,08223626 IMMP2L mitochondrial inner membrane protease subunit 2 7,35E-04 3,76E-04 1,954 1,718 0,08587296 Gm9093 60S acidic ribosomal protein P0 3,92E-04 1,91E-04 2,055 1,706 0,08804181 Aldoa1b aldolase 1 A retrogene 2 0,00106529 7,62E-04 1,397 1,693 0,09052281 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Ndufv1 precursor 0,001281086 9,46E-04 1,355 1,676 0,09366001 Carkd ATP-dependent (S)-NAD(P)H-hydrate dehydratase isoform 1 3,49E-04 1,76E-04 1,981 1,646 0,09974523 SLC30A9 zinc transporter 9 1,27E-04 4,47E-05 2,838 1,637 0,10166103 CYB5 cytochrome b5 5,77E-04 2,01E-04 2,867 1,637 0,10166103 Aldh16a1 aldehyde dehydrogenase family 16 member A1 1,32E-04 4,84E-05 2,718 1,637 0,10166103 5730469M10Rik redox-regulatory protein FAM213A 6,41E-04 3,71E-04 1,726 1,634 0,10225979 Gm4763 uncharacterized protein LOC210155 0,001832151 0,001107806 1,654 1,627 0,10378628 AKAP3 A-kinase anchor protein 3 0,003164035 0,002800195 1,130 1,614 0,10655301 H2-Ke6 estradiol 17-beta-dehydrogenase 8 0,001991804 0,001476132 1,349 1,605 0,1085284 LOC100048331 dnaJ homolog subfamily A member 4 3,00E-04 1,42E-04 2,106 1,594 0,11085998 DPEP3 dipeptidase 3 precursor 0,00255772 0,002020743 1,266 1,582 0,11367473 Fh1 fumarate hydratase, mitochondrial precursor 6,91E-04 4,77E-04 1,449 1,560 0,11866603 aldoa1a aldolase 1 A retrogene 1 5,38E-04 3,55E-04 1,514 1,560 0,11866603 PPA2 inorganic pyrophosphatase 2, mitochondrial precursor 3,69E-04 2,04E-04 1,810 1,549 0,12137303 Gm614 uncharacterized protein CXorf65 homolog 6,11E-04 3,14E-04 1,946 1,549 0,12137303 RTDR1 rhabdoid tumor deletion region gene 1 isoform 1 6,62E-04 4,29E-04 1,543 1,537 0,12419841 Tmem190 transmembrane protein 190 precursor 0,002831558 0,001909063 1,483 1,537 0,12419841 Gm9803 mitochondrial import inner membrane translocase subunit TIM16 4,75E-04 2,02E-04 2,351 1,527 0,12672999 STARD6 stAR-related lipid transfer protein 6 2,15E-04 6,34E-05 3,395 1,525 0,1273695 rpl11 60S ribosomal protein L11 2,49E-04 7,17E-05 3,467 1,525 0,1273695 Spam1 hyaluronidase PH-20 precursor 8,75E-04 6,42E-04 1,363 1,523 0,12773602 Acad10 acyl-CoA dehydrogenase family member 10 1,05E-04 5,24E-05 2,004 1,516 0,12964538 IMMP1L mitochondrial inner membrane protease subunit 1 6,20E-04 3,01E-04 2,061 1,516 0,12964538 Igbp1b immunoglobulin-binding protein 1b 9,37E-04 5,92E-04 1,582 1,501 0,13327969 Spesp1 sperm equatorial segment protein 1 precursor 0,008001396 0,007310148 1,095 1,488 0,13678875 ALDH9A1 4-trimethylaminobutyraldehyde dehydrogenase precursor 3,81E-04 2,45E-04 1,557 1,486 0,13729382 ribC1 RIB43A-like with coiled-coils protein 1 4,79E-04 2,78E-04 1,723 1,486 0,13729382 Sept7 septin-7 isoform 1 4,09E-04 2,69E-04 1,520 1,486 0,13729382 CCDC11 coiled-coil domain-containing protein 11 4,35E-04 2,97E-04 1,462 1,485 0,1375522 atp2b4 plasma membrane calcium ATPase 4 isoform a 0,001607779 0,001385159 1,161 1,474 0,14039434 Gm5604 keratin, type II cytoskeletal 8 4,08E-04 2,66E-04 1,538 1,463 0,14334653 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 PLCD4 isoform 1 2,48E-04 1,23E-04 2,015 1,461 0,14388984 Aldoa fructose-bisphosphate aldolase A isoform 1 precursor 0,001382077 0,001089093 1,269 1,461 0,14389376 Car2 carbonic anhydrase 2 0,001393316 0,001001324 1,391 1,455 0,14559456 Gm7591 voltage-dependent anion-selective channel protein 3 isoform 2 0,001540303 0,001127116 1,367 1,447 0,14791486 Glipr1l3 PREDICTED: GLIPR1-like protein 1-like 9,67E-04 6,11E-04 1,584 1,443 0,14911069 1700012P22Rik uncharacterized protein C1orf158 homolog 0,001073243 7,01E-04 1,531 1,443 0,14911069 1700009N14Rik uncharacterized protein LOC75471 2,99E-04 1,49E-04 2,011 1,442 0,14940561 Apoh beta-2-glycoprotein 1 precursor 2,21E-04 9,95E-05 2,221 1,442 0,14940561 GPX5 epididymal secretory glutathione peroxidase precursor 7,28E-04 4,20E-04 1,732 1,442 0,14943171 Ndufa8 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 0,002054993 0,001363709 1,507 1,429 0,15301074 Rnls renalase isoform 1 0,004247023 0,00329343 1,290 1,419 0,15594396 rpl12 60S ribosomal protein L12 8,58E-04 4,97E-04 1,726 1,413 0,15770832 Odf3b outer dense fiber protein 3B 9,23E-04 6,50E-04 1,420 1,406 0,15982805 LOC100044663 apolipoprotein O isoform 1 0,002018784 0,001488614 1,356 1,406 0,15985144 rps9 40S ribosomal protein S9 2,35E-04 8,54E-05 2,749 1,388 0,16512336 Slco6b1 solute carrier organic anion transporter family, member 6b1 1,43E-04 5,71E-05 2,509 1,388 0,16512336 Gm6685 cytochrome b5 domain-containing protein 1 6,20E-04 3,92E-04 1,581 1,375 0,16923315 BC051142 testis specific basic protein isoform 2 6,34E-04 3,18E-04 1,994 1,373 0,16977488 Gm13570 PREDICTED: mitochondrial pyruvate carrier-like protein-like 0,001739523 9,77E-04 1,781 1,363 0,17282663 LOC100047577 cytochrome b5 type B precursor 0,002243288 0,001506001 1,490 1,359 0,17407421 Uqcrc2 cytochrome b-c1 complex subunit 2, mitochondrial precursor 0,006359046 0,005865026 1,084 1,357 0,17478728 TCP1 T-complex protein 1 subunit alpha 0,00118338 9,71E-04 1,219 1,350 0,17701078 Aldoa2 fructose-bisphosphate aldolase A isoform 2 0,00128219 0,001000885 1,281 1,344 0,17886922 succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial oxct1 precursor 3,64E-04 2,49E-04 1,462 1,342 0,17972829 BCS1L mitochondrial chaperone BCS1 3,61E-04 2,13E-04 1,694 1,339 0,18047981 DDI1 protein DDI1 homolog 1 3,80E-04 1,99E-04 1,908 1,316 0,18804195 Tex101 testis-expressed sequence 101 protein precursor 0,002544181 0,001982425 1,283 1,285 0,19879741 NME7 nucleoside diphosphate kinase 7 isoform 1 5,85E-04 4,14E-04 1,414 1,271 0,20358833 Nme1 nucleoside diphosphate kinase A 7,43E-04 4,17E-04 1,780 1,269 0,20443782 SHMT1 serine hydroxymethyltransferase, cytosolic 2,27E-04 1,34E-04 1,696 1,269 0,20443782 - PREDICTED: uncharacterized protein C17orf47 homolog 0,001002824 8,09E-04 1,239 1,267 0,20530131 KRT1 keratin, type II cytoskeletal 1 9,27E-04 7,26E-04 1,278 1,248 0,21200577 snx3 sorting nexin-3 2,07E-04 7,41E-05 2,788 1,237 0,21610369 1700001P01Rik uncharacterized protein C17orf98 homolog 7,70E-04 4,32E-04 1,782 1,229 0,21918454 CES3 carboxylesterase 1D precursor 1,76E-04 9,21E-05 1,907 1,214 0,22458612 Angel1 protein angel homolog 2 isoform 2 1,58E-04 8,28E-05 1,904 1,214 0,22458612 PSMD6 26S proteasome non-ATPase regulatory subunit 6 1,57E-04 8,03E-05 1,956 1,214 0,22458612 BC089491 selenoprotein V 2,26E-04 1,32E-04 1,712 1,214 0,22458612 SPATA19 spermatogenesis-associated protein 19, mitochondrial precursor 0,001223858 7,75E-04 1,580 1,212 0,22537873 UQCRQ cytochrome b-c1 complex subunit 8 0,001939882 0,001226143 1,582 1,212 0,22537873 PSMB5 proteasome subunit beta type-5 0,001558533 0,00116698 1,336 1,192 0,2334054 Fam71b protein FAM71B 0,002599346 0,002246792 1,157 1,186 0,23577278 ATP6V1E2 V-type proton ATPase subunit E 2 3,04E-04 1,60E-04 1,902 1,155 0,24796304 Ssr4 translocon-associated protein subunit delta isoform 1 precursor 6,89E-04 4,04E-04 1,704 1,155 0,24796304 alb serum albumin precursor 7,37E-04 6,04E-04 1,219 1,152 0,24927587 ME1 NADP-dependent malic enzyme isoform 1 6,76E-04 4,99E-04 1,352 1,144 0,25271463 Sept12 septin-12 isoform 1 4,67E-04 3,17E-04 1,473 1,137 0,25541195 IL4I1 L-amino-acid oxidase precursor 0,001514632 0,001201265 1,261 1,124 0,26090843 LOC100044117 pyruvate dehydrogenase protein X component, mitochondrial 0,002480871 0,00215737 1,150 1,123 0,26127637 Pebp4 phosphatidylethanolamine-binding protein 4 isoform a precursor 0,001105914 7,31E-04 1,512 1,123 0,26164082

NDUFA12 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 0,002191033 0,001576319 1,390 1,121 0,26218849 Gm5617 uncharacterized protein C11orf71 homolog 4,01E-04 1,75E-04 2,290 1,117 0,26414425 TOMM34 mitochondrial import receptor subunit TOM34 1,11E-04 5,05E-05 2,188 1,117 0,26414425 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 RPN1 precursor 2,32E-04 1,52E-04 1,522 1,114 0,26532679 cpvl probable serine carboxypeptidase CPVL precursor 2,50E-04 1,50E-04 1,669 1,107 0,26809714 LOC100047329 40S ribosomal protein S18 4,23E-04 2,33E-04 1,818 1,107 0,26809714 SEC11A signal peptidase complex catalytic subunit SEC11A 4,18E-04 2,46E-04 1,699 1,107 0,26809714 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia NME5 nucleoside diphosphate kinase homolog 5 9,96E-04 6,78E-04 1,470 1,074 0,28262347 Psmc6 26S protease regulatory subunit 10B 2,14E-04 1,34E-04 1,594 1,068 0,28556232 HEATR7A HEAT repeat containing 7A isoform 1 2,08E-04 1,60E-04 1,300 1,067 0,28597642 Spa17 sperm surface protein Sp17 0,001619082 0,001169626 1,384 1,053 0,29225219 PDIA3 protein disulfide-isomerase A3 precursor 4,92E-04 3,95E-04 1,245 1,047 0,29511321 CATSPER4 cation channel sperm-associated protein 4 isoform 2 3,90E-04 2,62E-04 1,489 1,035 0,30081431 LOC100048611 ran guanine nucleotide release factor 6,80E-04 3,32E-04 2,049 1,033 0,30144183 acp1 low molecular weight phosphotyrosine protein phosphatase isoform 2 3,24E-04 1,70E-04 1,909 1,033 0,30144183 Lpcat2b lysophosphatidylcholine acyltransferase 2B 1,14E-04 6,25E-05 1,822 1,033 0,30144183 RAB10 ras-related protein Rab-10 2,06E-04 1,21E-04 1,705 1,033 0,30144183 Qrich2 glutamine rich 2 1,56E-04 7,93E-05 1,964 1,033 0,30144183 ARHGDIA rho GDP-dissociation inhibitor 1 3,01E-04 1,49E-04 2,026 1,033 0,30144183 TSSK4 testis-specific serine/threonine-protein kinase 4 isoform 1 9,21E-04 7,07E-04 1,302 1,026 0,30497238 Zmynd12 zinc finger, MYND domain containing 12 5,68E-04 3,82E-04 1,488 1,017 0,30938122 cpt2 carnitine O-palmitoyltransferase 2, mitochondrial precursor 6,61E-04 5,11E-04 1,292 1,006 0,31438824 PSMC2 26S protease regulatory subunit 7 3,19E-04 2,37E-04 1,343 1,005 0,31479968 1700016C15Rik uncharacterized protein C1orf100 homolog 0,001110394 7,25E-04 1,531 0,982 0,32605394 NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial ndufs2 precursor 0,001250118 0,0010109 1,237 0,978 0,32802838 ME2 NAD-dependent malic enzyme, mitochondrial precursor 4,32E-04 3,40E-04 1,270 0,977 0,32871459 PPP6C serine/threonine-protein phosphatase 6 catalytic subunit 2,03E-04 1,22E-04 1,666 0,972 0,33106408 BLVRB flavin reductase (NADPH) 3,28E-04 2,06E-04 1,591 0,972 0,33106408 Gm10171 mitochondrial import inner membrane translocase subunit Tim23 4,86E-04 3,00E-04 1,620 0,972 0,33106408 LOC100044315 importin-5 8,30E-05 4,79E-05 1,733 0,972 0,33106408 C8orfk29 PREDICTED: putative transmembrane protein C8orfK29 2,42E-04 1,54E-04 1,578 0,972 0,33106408 Dusp21 dual specificity phosphatase 21 8,71E-04 5,50E-04 1,586 0,972 0,33106408 GPX6 glutathione peroxidase 6 precursor 4,08E-04 2,43E-04 1,677 0,972 0,33106408 hibadh 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor 0,002031992 0,0017461 1,164 0,966 0,33408241 Gm9047 uncharacterized protein LOC668210 0,00118929 9,20E-04 1,292 0,955 0,33978002 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial NDUFS1 precursor 0,002302508 0,002090475 1,101 0,947 0,34366211 SLC25A20 mitochondrial carnitine/acylcarnitine carrier protein 9,53E-04 7,23E-04 1,319 0,932 0,35118297 FSCN3 fascin-3 4,13E-04 3,08E-04 1,340 0,926 0,35432053 SLC22A14 solute carrier family 22 member 14 2,18E-04 1,39E-04 1,572 0,925 0,35506459 succinate dehydrogenase cytochrome b560 subunit, mitochondrial sdhC precursor 6,44E-04 4,30E-04 1,499 0,925 0,35506459 rab5c ras-related protein Rab-5C 0,001259646 0,001075005 1,172 0,923 0,35575065 Adam4 a disintegrin and metallopeptidase domain 4 precursor 2,34E-04 1,74E-04 1,344 0,912 0,36185106 Gm12164 ferritin light chain 1 1,68E-04 8,02E-05 2,098 0,912 0,36191301 arl1 ADP-ribosylation factor-like protein 1 1,88E-04 8,36E-05 2,244 0,912 0,36191301 PPIF peptidyl-prolyl cis-trans isomerase F, mitochondrial precursor 1,25E-04 6,24E-05 2,000 0,912 0,36191301 FDX1L adrenodoxin-like protein, mitochondrial precursor 1,80E-04 8,18E-05 2,197 0,912 0,36191301 tk2 thymidine kinase 2, mitochondrial precursor 1,34E-04 6,38E-05 2,103 0,912 0,36191301 Ndufb7 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 0,001873144 0,001570406 1,193 0,907 0,36462992 NUP210L nuclear pore membrane glycoprotein 210-like precursor 2,31E-04 1,96E-04 1,182 0,904 0,3660428 CCDC147 coiled-coil domain containing 147 7,46E-04 6,41E-04 1,163 0,894 0,37131837 PREDICTED: NADH dehydrogenase [ubiquinone] 1 beta subcomplex Gm3244 subunit 4-like 0,001594838 0,001194145 1,336 0,888 0,37475647 p4hb protein disulfide-isomerase precursor 1,36E-04 8,18E-05 1,660 0,887 0,37483619 pyruvate dehydrogenase E1 component subunit beta, mitochondrial Gm6123 precursor 0,008956886 0,008671776 1,033 0,881 0,37808723 Tuba3a tubulin alpha-3 chain 0,001872541 0,00171741 1,090 0,878 0,37996697 Tubb2a-ps2 tubulin beta-2B chain 3,14E-04 2,49E-04 1,260 0,868 0,38532141 1700018L24Rik testis-expressed sequence 29 protein 0,00116219 9,22E-04 1,261 0,868 0,38532141 cutC copper homeostasis protein cutC homolog isoform 2 6,90E-04 5,70E-04 1,210 0,852 0,39403195 Nnt NAD(P) transhydrogenase, mitochondrial precursor 3,50E-04 2,72E-04 1,289 0,834 0,4043856 - PREDICTED: WD repeat-containing protein 66 2,18E-04 1,63E-04 1,339 0,829 0,40724613 NDUFB10 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 0,001005548 7,95E-04 1,265 0,829 0,40724613 CST11 cystatin-11 precursor 2,49E-04 1,33E-04 1,872 0,817 0,41398922 Gm7308 calmodulin 0,001805506 0,001316415 1,372 0,807 0,41981576 Dnahc10 dynein heavy chain 10, axonemal 6,62E-05 5,44E-05 1,218 0,804 0,4213009 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, Ndufb8 mitochondrial precursor 0,001440395 0,001206639 1,194 0,802 0,42264663 Defb22 beta-defensin 22 precursor 0,001934416 0,001486792 1,301 0,800 0,4238632 TXNDC3 thioredoxin domain-containing protein 3 isoform 1 0,001099758 9,44E-04 1,166 0,790 0,42951708 Pcx pyruvate carboxylase, mitochondrial isoform 2 4,50E-04 3,94E-04 1,141 0,790 0,42951708 coiled-coil-helix-coiled-coil-helix domain-containing protein 6, CHCHD6 mitochondrial isoform 1 0,001403999 0,001205515 1,165 0,789 0,42990932 Gm9646 40S ribosomal protein S19 2,31E-04 1,50E-04 1,539 0,755 0,45019834 rps13 40S ribosomal protein S13 2,56E-04 1,50E-04 1,700 0,755 0,45019834 C030006K11Rik UPF0598 protein C8orf82 homolog isoform 1 2,11E-04 1,36E-04 1,554 0,755 0,45019834 - uncharacterized protein LOC627607 1,07E-04 6,71E-05 1,593 0,755 0,45019834 Epb4.2 erythrocyte membrane protein band 4.2 9,17E-05 5,59E-05 1,640 0,755 0,45019834 Gm8219 mitochondrial pyruvate carrier 1 5,46E-04 3,18E-04 1,721 0,755 0,45019834 Atp5d ATP synthase subunit delta, mitochondrial precursor 0,001250396 9,87E-04 1,266 0,754 0,45063817 mdh2 malate dehydrogenase, mitochondrial precursor 0,004014748 0,003779323 1,062 0,752 0,45186476 1700071K01Rik prohibitin 0,002307591 0,002115717 1,091 0,747 0,45523905 CCDC81 coiled-coil domain-containing protein 81 2,01E-04 1,60E-04 1,255 0,745 0,45651966 Arpm1 actin-related protein T3 4,61E-04 3,64E-04 1,268 0,745 0,45651966 KLHL10 kelch-like protein 10 2,44E-04 2,03E-04 1,203 0,729 0,4662432 Efcab2 EF-hand calcium-binding domain-containing protein 2 4,34E-04 2,93E-04 1,483 0,712 0,47671323 CATSPER1 cation channel sperm-associated protein 1 2,11E-04 1,43E-04 1,482 0,712 0,47671323 DPP4 dipeptidyl peptidase 4 isoform 2 8,82E-05 5,79E-05 1,523 0,712 0,47671323 Ubtd2 ubiquitin domain-containing protein 2 2,92E-04 2,02E-04 1,446 0,712 0,47671323 Cd59b CD59B glycoprotein precursor 3,19E-04 2,22E-04 1,440 0,712 0,47671323 EFHC2 EF-hand domain-containing family member C2 8,24E-04 6,87E-04 1,198 0,709 0,47831606 Gm3982 mitochondrial pyruvate carrier 2 0,005065002 0,004475488 1,132 0,697 0,48585555 1190002A17Rik adenylate kinase 8 6,97E-04 6,24E-04 1,116 0,685 0,49349573 Samm50 sorting and assembly machinery component 50 homolog 7,55E-04 6,71E-04 1,125 0,681 0,4956013 PMM2 phosphomannomutase 2 1,98E-04 1,29E-04 1,531 0,679 0,49684477 PPM1B protein phosphatase 1B isoform 1 1,44E-04 9,86E-05 1,458 0,679 0,49684477 vps25 vacuolar protein-sorting-associated protein 25 3,93E-04 2,81E-04 1,396 0,655 0,51253475 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia TBL1XR1 F-box-like/WD repeat-containing protein TBL1XR1 1,98E-04 1,43E-04 1,383 0,655 0,51253475 AMDHD2 putative N-acetylglucosamine-6-phosphate deacetylase 2,37E-04 1,70E-04 1,391 0,655 0,51253475 mitochondrial translocator assembly and maintenance protein 41 homolog 1500001M20Rik precursor 2,94E-04 2,34E-04 1,254 0,655 0,51253475 ASRGL1 isoaspartyl peptidase/L-asparaginase 0,001637608 0,001455404 1,125 0,647 0,51746929 4921536K21Rik uncharacterized protein C5orf52 homolog 4,44E-04 3,19E-04 1,390 0,636 0,52500177

NDUFA11 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 8,59E-04 6,15E-04 1,397 0,636 0,52500177 TIMM17B mitochondrial import inner membrane translocase subunit Tim17-B 5,32E-04 4,09E-04 1,302 0,636 0,52500177 4930504O13Rik uncharacterized protein LOC403200 precursor 5,98E-04 4,18E-04 1,430 0,636 0,52500177 PSMD12 26S proteasome non-ATPase regulatory subunit 12 1,86E-04 1,37E-04 1,360 0,620 0,53505183 Tceb2 transcription elongation factor B polypeptide 2 7,54E-04 6,04E-04 1,248 0,620 0,53505183 cct4 T-complex protein 1 subunit delta 9,10E-04 7,93E-04 1,149 0,614 0,53906569 Gm13050 elongation factor 2 1,07E-04 8,08E-05 1,321 0,608 0,54324079 Cd55 complement decay-accelerating factor, GPI-anchored precursor 4,34E-04 3,43E-04 1,265 0,590 0,55551628 SPACA3 sperm acrosome membrane-associated protein 3 precursor 9,07E-04 6,80E-04 1,334 0,583 0,56011259 BC048562 uncharacterized protein LOC434439 0,00108912 8,32E-04 1,309 0,577 0,56392192 Trap1 heat shock protein 75 kDa, mitochondrial precursor 1,85E-04 1,54E-04 1,204 0,572 0,56707514 4931409K22Rik IQ and AAA domain-containing protein 1-like 1,47E-04 1,18E-04 1,243 0,572 0,56707514 rps3 40S ribosomal protein S3 3,71E-04 2,93E-04 1,265 0,572 0,56707514 1700019N12Rik testis-expressed sequence 40 protein 9,84E-04 7,85E-04 1,254 0,565 0,57180764 1700026D08Rik uncharacterized protein C15orf26 homolog 0,001219064 9,99E-04 1,220 0,565 0,57201539 Sept4 septin-4 6,43E-04 5,81E-04 1,105 0,565 0,57201539 bat3 large proline-rich protein BAG6 isoform 1 6,06E-04 5,45E-04 1,112 0,564 0,57291147 Ribc2 RIB43A-like with coiled-coils protein 2 3,84E-04 3,29E-04 1,168 0,563 0,57353782 MNS1 meiosis-specific nuclear structural protein 1 4,43E-04 3,88E-04 1,142 0,560 0,57528296 Pfn3 profilin-3 0,0010318 8,60E-04 1,200 0,559 0,5760064 mitochondrial import inner membrane translocase subunit TIM50 timm50 precursor 5,86E-04 5,00E-04 1,172 0,559 0,5760064 MLF1 myeloid leukemia factor 1 isoform a 6,98E-04 6,29E-04 1,109 0,557 0,57781583 NUP155 nuclear pore complex protein Nup155 2,72E-04 2,33E-04 1,167 0,556 0,5780037 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, Ndufb5 mitochondrial precursor 0,001097814 8,55E-04 1,284 0,556 0,5780304 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial isoform Ndufv2 1 precursor 0,002059612 0,001897147 1,086 0,535 0,59251011 pnpla8 calcium-independent phospholipase A2-gamma 3,25E-05 1,96E-05 1,659 0,534 0,59340302 bag1 BAG family molecular chaperone regulator 1 isoform 1L 8,14E-05 4,52E-05 1,802 0,534 0,59340302 ASAH1 acid ceramidase precursor 6,35E-05 4,12E-05 1,541 0,534 0,59340302 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, PGS1 mitochondrial 5,70E-05 3,74E-05 1,523 0,534 0,59340302 LY6G5B lymphocyte antigen 6 complex locus protein G5b precursor 1,87E-04 1,05E-04 1,786 0,534 0,59340302 CIB1 calcium and integrin-binding protein 1 1,07E-04 6,90E-05 1,556 0,534 0,59340302 Gm8808 PREDICTED: 60S ribosomal protein L30-like 1,83E-04 1,10E-04 1,661 0,534 0,59340302 actn4 alpha-actinin-4 2,56E-05 1,60E-05 1,600 0,534 0,59340302 Capzb F-actin-capping protein subunit beta isoform c 0,001407936 0,001223566 1,151 0,519 0,60386163 Car4 carbonic anhydrase 4 precursor 0,001913745 0,001673315 1,144 0,508 0,61162847 Gm16399 cytochrome c oxidase subunit 6C 0,005556151 0,005040575 1,102 0,505 0,61359494 BANF2 barrier-to-autointegration factor-like protein 2,23E-04 1,52E-04 1,467 0,480 0,63091827 COPS7A COP9 signalosome complex subunit 7a isoform 2 1,21E-04 9,09E-05 1,331 0,480 0,63091827 USP5 ubiquitin carboxyl-terminal hydrolase 5 4,29E-05 3,10E-05 1,384 0,480 0,63091827 Defb2 beta-defensin 2 precursor 8,21E-04 6,12E-04 1,341 0,480 0,63091827 Gm5081 ribose-phosphate pyrophosphokinase 1 1,06E-04 7,62E-05 1,394 0,480 0,63091827 - predicted gene 6760 2,59E-04 1,64E-04 1,577 0,480 0,63091827 Dhrs4 dehydrogenase/reductase SDR family member 4 isoform 1 0,001236229 0,001091554 1,133 0,476 0,63377824 Naglu alpha-N-acetylglucosaminidase precursor 9,87E-04 9,36E-04 1,054 0,451 0,65222583 4933401F05Rik serine protease 55 precursor 3,31E-04 2,56E-04 1,294 0,449 0,6531042 gpX3 glutathione peroxidase 3 precursor 2,00E-04 1,57E-04 1,273 0,449 0,6531042 2310061I04Rik uncharacterized protein LOC69662 1,95E-04 1,43E-04 1,365 0,449 0,6531042 TMEM30A cell cycle control protein 50A 1,56E-04 1,17E-04 1,331 0,449 0,6531042 RPS25 PREDICTED: 40S ribosomal protein S25-like 2,01E-04 1,67E-04 1,204 0,449 0,6531042 PREDICTED: mitochondrial import inner membrane translocase subunit Tim14 TIM14-like 2,74E-04 1,95E-04 1,408 0,449 0,6531042 MORN2 MORN repeat-containing protein 2 4,33E-04 3,44E-04 1,259 0,449 0,6531042 Prss52 serine protease 52 precursor 1,59E-04 1,26E-04 1,266 0,449 0,6531042 H2afb1 histone H2A-Bbd type 1 3,51E-04 2,75E-04 1,279 0,449 0,6531042 vamp3 vesicle-associated membrane protein 3 0,003036904 0,002745097 1,106 0,433 0,66520437 Gm12231 ATP synthase subunit b, mitochondrial precursor 0,001680577 0,001575675 1,067 0,430 0,6669752 CAPZA3 F-actin-capping protein subunit alpha-3 8,42E-04 7,55E-04 1,114 0,430 0,66728348 FABP4 fatty acid-binding protein, adipocyte 2,44E-04 1,90E-04 1,282 0,430 0,66731278 Gm216 uncharacterized protein C2orf62 homolog 1,55E-04 1,18E-04 1,321 0,430 0,66731278 Gm9840 E3 ubiquitin-protein ligase RBX1 6,26E-04 5,03E-04 1,246 0,430 0,66731278 1700019N19Rik uncharacterized protein C10orf82 homolog 0,001456851 0,001282816 1,136 0,427 0,66934886 SDR39U1 epimerase family protein SDR39U1 0,001030104 8,53E-04 1,207 0,424 0,6713969 SCAMP2 secretory carrier-associated membrane protein 2 0,002760458 0,002428249 1,137 0,421 0,67342344 Erlin2 erlin-2 6,24E-04 5,48E-04 1,140 0,419 0,67542362 synj2bp synaptojanin-2-binding protein 4,21E-04 3,38E-04 1,247 0,417 0,67680946 AGPAT2 1-acyl-sn-glycerol-3-phosphate acyltransferase beta precursor 2,40E-04 1,83E-04 1,312 0,417 0,67680946 FKBP1A peptidyl-prolyl cis-trans isomerase FKBP1A 5,08E-04 4,14E-04 1,227 0,417 0,67680946 [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, Pdp1 mitochondrial isoform b 1,39E-04 1,11E-04 1,253 0,408 0,68328361 1700071K01Rik prohibitin-like 5,37E-04 4,96E-04 1,082 0,404 0,68647239 GSTT3 glutathione S-transferase, theta 3 3,76E-04 2,97E-04 1,265 0,402 0,68770296 LOC641192 heat shock cognate 71 kDa protein 6,70E-04 6,27E-04 1,067 0,402 0,68801598

NDUFS4 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial 6,77E-04 6,09E-04 1,112 0,401 0,68805707 trim69 E3 ubiquitin-protein ligase TRIM69 3,03E-04 2,66E-04 1,139 0,399 0,68952668 1110032A03Rik UPF0686 protein C11orf1 homolog 6,55E-04 5,89E-04 1,113 0,398 0,69066404 Hmox2 heme oxygenase 2 4,61E-04 3,95E-04 1,168 0,398 0,69085764 prcp lysosomal Pro-X carboxypeptidase precursor 4,81E-04 4,19E-04 1,148 0,398 0,69085764 LOC100045699 electron transfer flavoprotein subunit beta 4,26E-04 3,58E-04 1,191 0,398 0,69085764 psmb2 proteasome subunit beta type-2 7,15E-04 6,42E-04 1,114 0,396 0,69202089 LRRC34 leucine-rich repeat-containing protein 34 2,42E-04 2,23E-04 1,087 0,394 0,69364149 PPP3R2 calcineurin subunit B type 2 4,81E-04 4,08E-04 1,179 0,394 0,69364149 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia SSBP1 single-stranded DNA-binding protein, mitochondrial isoform 1 6,62E-04 5,46E-04 1,213 0,393 0,69409108 Lrrc51 leucine-rich repeat-containing protein 51 isoform 1 6,06E-04 5,13E-04 1,183 0,393 0,6941084 Got2 aspartate aminotransferase, mitochondrial 9,96E-04 8,88E-04 1,121 0,386 0,69921951 Thnsl1 threonine synthase-like 1 isoform a 5,47E-04 4,98E-04 1,099 0,375 0,70796407 cul3 cullin-3 3,99E-04 3,65E-04 1,093 0,375 0,70796407 Tssk1 testis-specific serine/threonine-protein kinase 1 7,78E-04 7,00E-04 1,112 0,346 0,72968324 sccpdh saccharopine dehydrogenase-like oxidoreductase 0,003381013 0,00315341 1,072 0,343 0,73179218 Gykl1 glycerol kinase-like 1 0,002102319 0,00199479 1,054 0,329 0,7418144 Dnaic1 dynein intermediate chain 1, axonemal 9,93E-04 9,75E-04 1,019 0,326 0,74408322 DNPEP aspartyl aminopeptidase isoform b 0,002070523 0,002109802 1,019 0,325 0,74548579 gtl3 UPF0468 protein C16orf80 homolog 0,001439794 0,001235519 1,165 0,319 0,75006155 Adam6b a disintegrin and metalloproteinase domain 6-like 3,67E-04 3,48E-04 1,054 0,289 0,77252901 Pgk1-rs7 phosphoglycerate kinase 1 3,96E-04 3,70E-04 1,070 0,273 0,78474922 CCT8L1 chaperonin containing TCP1, subunit 8 (theta)-like 1 3,95E-04 3,54E-04 1,114 0,256 0,79778448 1700011F03Rik testis-expressed sequence 37 protein 0,001214217 0,001042771 1,164 0,256 0,79778448 atp5s ATP synthase subunit s, mitochondrial precursor 7,75E-04 6,53E-04 1,186 0,250 0,80233835 RSPH9 radial spoke head protein 9 homolog 0,002076994 0,00200432 1,036 0,244 0,80686854 Atp1b3 sodium/potassium-transporting ATPase subunit beta-3 0,001354716 0,001287394 1,052 0,236 0,81307578 PRSS1 protease, serine, 1 precursor 0,002423699 0,002100943 1,154 0,231 0,81751778 lrrc48 leucine-rich repeat-containing protein 48 3,14E-04 2,93E-04 1,072 0,225 0,82181281 HTRA1 serine protease HTRA1 precursor 3,28E-04 2,96E-04 1,108 0,225 0,82181281 morn5 MORN repeat-containing protein 5 7,41E-04 6,92E-04 1,071 0,225 0,82181281 TSSK6 testis-specific serine/threonine-protein kinase 6 4,42E-04 3,77E-04 1,173 0,218 0,82704942 IDH3B isocitrate dehydrogenase 3, beta subunit 0,002057599 0,001848197 1,113 0,211 0,83306508 PSMB6 proteasome subunit beta type-6 precursor 5,19E-04 5,02E-04 1,035 0,204 0,83806293 slc25a1 solute carrier family 25, member 1 3,48E-04 3,18E-04 1,093 0,197 0,84387797 - PREDICTED: uncharacterized protein LOC100862214 3,97E-04 3,82E-04 1,039 0,197 0,84387797 NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial NDUFS7 precursor 0,001971037 0,002011823 1,021 0,192 0,84779037

Ms4a14 PREDICTED: membrane-spanning 4-domains subfamily A member 14 2,15E-04 1,98E-04 1,084 0,189 0,84993015 Uqcrh cytochrome b-c1 complex subunit 6, mitochondrial 9,26E-04 7,83E-04 1,182 0,181 0,85624944 Gm6293 cytochrome b-c1 complex subunit 9 0,001483799 0,001484117 1,000 0,181 0,85624944 LOC100045796 cysteine desulfurase, mitochondrial 4,91E-04 4,71E-04 1,043 0,174 0,8622336 CATSPER2 cation channel sperm-associated protein 2 2,51E-04 2,14E-04 1,174 0,173 0,86287278 BC048507 dynein light chain LC8-type 1-like 7,40E-04 6,35E-04 1,165 0,173 0,86287278 serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A PPP2R1A alpha isoform 1,45E-04 1,34E-04 1,086 0,173 0,86287278 HINT2 histidine triad nucleotide-binding protein 2, mitochondrial precursor 3,98E-04 3,57E-04 1,114 0,164 0,86984661 SATL1 spermidine/spermine N(1)-acetyltransferase-like protein 1 1,73E-04 1,55E-04 1,118 0,164 0,86984661 FAM78A protein FAM78A 3,88E-04 3,63E-04 1,068 0,164 0,86984661 Gm7795 cytochrome c oxidase subunit 6A1, mitochondrial 0,001494591 0,00143545 1,041 0,164 0,86984661 rpiA ribose-5-phosphate isomerase 2,27E-04 2,20E-04 1,033 0,164 0,86984661 LOC100045163 phospholipase B-like 1 precursor 5,38E-04 5,09E-04 1,056 0,159 0,8736315 ACYP1 acylphosphatase-1 4,84E-04 4,66E-04 1,038 0,154 0,87723069 4921517D21Rik uncharacterized protein LOC67722 7,88E-05 7,54E-05 1,044 0,154 0,87723069 DNAJA3 dnaJ homolog subfamily A member 3, mitochondrial isoform 1 1,28E-04 1,20E-04 1,070 0,154 0,87723069 abhd11 alpha/beta hydrolase domain-containing protein 11 isoform 1 3,42E-04 3,02E-04 1,132 0,154 0,87723069 2410015M20Rik protein QIL1 precursor 6,53E-04 6,05E-04 1,078 0,154 0,87723069 1700007I08Rik actin-like protein 10 2,33E-04 2,18E-04 1,067 0,154 0,87723069 SPINT4 kunitz-type protease inhibitor 4 precursor 4,02E-04 3,71E-04 1,086 0,154 0,87723069 4922502D21Rik C-type lectin-like receptor 2m 3,47E-04 3,27E-04 1,062 0,154 0,87723069 PSMD14 26S proteasome non-ATPase regulatory subunit 14 2,89E-04 2,71E-04 1,070 0,145 0,8851041 ASB9 ankyrin repeat and SOCS box protein 9 3,48E-04 3,19E-04 1,092 0,145 0,8851041 LOC100045999 PREDICTED: GTP-binding nuclear protein Ran-like 2,48E-04 2,42E-04 1,023 0,145 0,8851041 LOC100047986 elongation factor 1-gamma 5,65E-04 5,52E-04 1,024 0,144 0,88572724 Psma3 proteasome subunit alpha type-3 6,92E-04 6,37E-04 1,088 0,144 0,88572724 Dnahc17 dynein heavy chain 17, axonemal 4,03E-04 3,77E-04 1,068 0,141 0,88817414 isyna1 inositol-3-phosphate synthase 1 1,37E-04 1,31E-04 1,051 0,134 0,89357542

ALDH4A1 delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial precursor 9,78E-05 9,16E-05 1,068 0,134 0,89357542 - UPF0704 protein C6orf165 homolog 1,08E-04 1,03E-04 1,055 0,134 0,89357542 NDUFA1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 5,93E-04 5,57E-04 1,066 0,134 0,89357542 HIST2H3B histone H2A type 2-A 3,95E-04 3,86E-04 1,022 0,134 0,89357542 LOC100047501 40S ribosomal protein S16 2,44E-04 2,30E-04 1,060 0,134 0,89357542 1700019L03Rik uncharacterized protein C9orf117 homolog 8,06E-05 7,60E-05 1,061 0,122 0,90280092 KCNU1 potassium channel subfamily U member 1 6,12E-05 6,23E-05 1,019 0,122 0,90280092 Antxrl anthrax toxin receptor-like precursor 9,19E-05 8,32E-05 1,105 0,122 0,90280092 SPCS3 signal peptidase complex subunit 3 2,61E-04 2,16E-04 1,206 0,122 0,90280092 Efcab9 EF-hand calcium-binding domain-containing protein 9 2,47E-04 2,33E-04 1,060 0,122 0,90280092 gdpd1 glycerophosphodiester phosphodiesterase domain-containing protein 1 1,17E-04 1,13E-04 1,037 0,122 0,90280092 Smpd5 sphingomyelin phosphodiesterase 5 1,38E-04 1,27E-04 1,083 0,122 0,90280092 carhsp1 calcium-regulated heat stable protein 1 2,99E-04 2,55E-04 1,172 0,122 0,90280092 Txndc2 thioredoxin domain-containing protein 2 isoform 2 8,29E-05 7,31E-05 1,134 0,122 0,90280092 PSMB4 proteasome subunit beta type-4 precursor 8,63E-04 8,27E-04 1,043 0,119 0,90543372 them4 acyl-coenzyme A thioesterase THEM4 8,90E-04 8,67E-04 1,026 0,110 0,91249341 9230107M04Rik binder of sperm protein homolog 2 precursor 3,00E-04 3,00E-04 1,000 0,109 0,91302027 Abca14 ATP-binding cassette, sub-family A (ABC1), member 14 2,62E-05 2,33E-05 1,128 0,109 0,91302027 iscA1 iron-sulfur cluster assembly 1 homolog, mitochondrial precursor 1,89E-04 1,69E-04 1,120 0,109 0,91302027 Lman2 vesicular integral-membrane protein VIP36 precursor 9,02E-05 8,16E-05 1,106 0,109 0,91302027 prdx3 thioredoxin-dependent peroxide reductase, mitochondrial precursor 1,49E-04 1,39E-04 1,070 0,109 0,91302027 pdcd6 programmed cell death protein 6 1,93E-04 1,75E-04 1,102 0,109 0,91302027 Rab1 ras-related protein Rab-1A 1,23E-04 1,12E-04 1,100 0,109 0,91302027 LOC100046373 ras-related protein Rap-1A precursor 1,59E-04 1,38E-04 1,150 0,109 0,91302027

Cox7c PREDICTED: cytochrome c oxidase subunit 7C, mitochondrial-like 4,92E-04 4,56E-04 1,078 0,109 0,91302027

Cabyr calcium-binding tyrosine phosphorylation-regulated protein isoform c 9,29E-05 8,22E-05 1,130 0,095 0,92463673 Oit1 protein FAM3D precursor 1,02E-04 9,47E-05 1,072 0,095 0,92463673 Crisp2 cysteine-rich secretory protein 2 precursor 1,40E-04 1,20E-04 1,166 0,095 0,92463673 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 Rpn2 precursor 5,91E-05 5,67E-05 1,042 0,095 0,92463673 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia Spag8 sperm-associated antigen 8 8,71E-05 8,42E-05 1,035 0,095 0,92463673 Krt42 keratin, type I cytoskeletal 42 6,42E-05 5,81E-05 1,104 0,095 0,92463673 SPATA17 spermatogenesis-associated protein 17 isoform 1 5,75E-05 4,78E-05 1,202 0,095 0,92463673 CATSPER3 cation channel sperm-associated protein 3 isoform 2 1,31E-04 1,11E-04 1,180 0,095 0,92463673 Mlec malectin precursor 7,66E-05 6,91E-05 1,109 0,095 0,92463673 1700094D03Rik uncharacterized protein C1orf189 homolog 2,15E-04 1,76E-04 1,226 0,095 0,92463673 Gm13082 ras-related protein Rab-6A isoform 2 9,05E-05 8,23E-05 1,100 0,095 0,92463673 lyrm1 LYR motif-containing protein 1 1,97E-04 1,91E-04 1,031 0,095 0,92463673 Ranbp3 ran-binding protein 3 isoform 1 4,31E-05 3,95E-05 1,092 0,077 0,93843624 JAGN1 protein jagunal homolog 1 isoform 1 1,61E-04 1,66E-04 1,029 0,077 0,93843624 BRI3BP BRI3-binding protein precursor 1,15E-04 1,07E-04 1,077 0,077 0,93843624 Gm5428 PREDICTED: 60S ribosomal protein L6-like 4,12E-05 3,80E-05 1,082 0,077 0,93843624 EFCAB3 EF-hand calcium-binding domain-containing protein 3 3,49E-05 3,22E-05 1,083 0,077 0,93843624 Lrrc52 leucine-rich repeat-containing protein 52 precursor 9,17E-05 9,33E-05 1,018 0,077 0,93843624 Qsox1 sulfhydryl oxidase 1 isoform b precursor 2,87E-05 2,83E-05 1,015 0,077 0,93843624 HDHD3 haloacid dehalogenase-like hydrolase domain-containing protein 3 9,91E-05 9,23E-05 1,073 0,077 0,93843624 DKKL1 dickkopf-like protein 1 precursor 6,88E-05 5,86E-05 1,174 0,077 0,93843624 Ces7 carboxylesterase 5A precursor 5,22E-04 5,30E-04 1,016 0,070 0,94388342 Psmd11 26S proteasome non-ATPase regulatory subunit 11 2,80E-04 2,69E-04 1,040 0,048 0,9620046

Gdpd4 glycerophosphodiester phosphodiesterase domain-containing protein 4 4,13E-04 4,00E-04 1,032 0,048 0,9620046

Ndufs8 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial 0,001070616 0,001089685 1,018 0,041 0,96749766 Slc22a21 solute carrier family 22 member 21 4,61E-04 4,77E-04 1,035 0,035 0,97185928 PRKAR1A cAMP-dependent protein kinase type I-alpha regulatory subunit 9,14E-04 9,20E-04 1,006 0,022 0,98221792 FANK1 fibronectin type 3 and ankyrin repeat domains 1 protein 2,80E-04 2,85E-04 1,017 0,022 0,9823458 Lrrc57 leucine-rich repeat-containing protein 57 isoform b 4,37E-04 4,29E-04 1,019 0,022 0,9823458 LOC100046727 IQ and ubiquitin-like domain-containing protein 1,58E-04 1,57E-04 1,008 0,022 0,9823458 Gm12906 mitochondrial import receptor subunit TOM22 homolog 0,001089806 0,001030579 1,057 0,022 0,9823458 LCN12 epididymal-specific lipocalin-12 precursor 0,001122518 0,001101436 1,019 0,022 0,9823458 Dynlrb2 dynein light chain roadblock-type 2 9,23E-04 8,66E-04 1,066 0,008 0,99356357 CCDC151 coiled-coil domain-containing protein 151 isoform 2 4,40E-04 4,25E-04 1,037 0,005 0,99564443 Tmco2 transmembrane and coiled-coil domain-containing protein 2 8,64E-04 8,70E-04 1,006 -0,007 0,99436262 9430016H08Rik uncharacterized protein C2orf47 homolog, mitochondrial precursor 3,11E-04 3,25E-04 1,045 -0,007 0,99436262 etfA electron transfer flavoprotein subunit alpha, mitochondrial 2,58E-04 2,53E-04 1,022 -0,007 0,99436262 RAB18 ras-related protein Rab-18 isoform 2 precursor 3,36E-04 3,45E-04 1,026 -0,007 0,99436262 PGRMC1 membrane-associated progesterone receptor component 1 4,52E-04 4,53E-04 1,003 -0,007 0,99436262 LYZL6 lysozyme-like protein 6 precursor 0,001642407 0,001485757 1,105 -0,007 0,99436262 Cct7 T-complex protein 1 subunit eta 6,72E-04 6,52E-04 1,030 -0,014 0,98872102 C1orf101 PREDICTED: uncharacterized protein C1orf101-like 1,29E-04 1,29E-04 1,002 -0,041 0,96696086 4933411K16Rik uncharacterized protein C10orf62 homolog 9,05E-04 9,01E-04 1,004 -0,046 0,96363613 LDHAL6B L-lactate dehydrogenase A-like 6B 0,006196426 0,006514982 1,051 -0,055 0,95646149 RANBP17 ran-binding protein 17 5,06E-04 5,08E-04 1,005 -0,060 0,95196145 Dbil5 diazepam-binding inhibitor-like 5 0,002043386 0,001896206 1,078 -0,061 0,9513089 NDUFB3 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 6,08E-04 6,78E-04 1,114 -0,061 0,9511476 TAGLN transgelin 3,06E-04 3,09E-04 1,012 -0,061 0,9511476 NDUFB6 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 5,14E-04 5,56E-04 1,080 -0,061 0,9511476 Samd15 PREDICTED: sterile alpha motif domain-containing protein 15 1,24E-04 1,24E-04 1,001 -0,061 0,9511476 PSMC5 26S protease regulatory subunit 8 3,06E-04 2,95E-04 1,038 -0,072 0,94240769 GAS8 growth arrest-specific protein 8 4,35E-04 4,21E-04 1,033 -0,083 0,93399779 BANF1 barrier-to-autointegration factor 6,42E-04 6,22E-04 1,032 -0,084 0,93345211 aqp7 aquaporin-7 4,65E-04 5,49E-04 1,183 -0,084 0,93345211 DHRS7B dehydrogenase/reductase SDR family member 7B isoform 2 4,69E-04 4,71E-04 1,003 -0,087 0,93094849

Adam1b disintegrin and metalloproteinase domain-containing protein 1b precursor 2,65E-04 2,75E-04 1,039 -0,102 0,91883619 ddt D-dopachrome decarboxylase 9,46E-04 9,21E-04 1,026 -0,102 0,91883619 PHB2 prohibitin-2 0,002292094 0,002392801 1,044 -0,102 0,91872465 Cct3 T-complex protein 1 subunit gamma 0,001321618 0,001324683 1,002 -0,102 0,91872465 CAND1 cullin-associated NEDD8-dissociated protein 1 2,89E-04 2,97E-04 1,026 -0,102 0,91860569 ANKRD5 ankyrin repeat and EF-hand domain-containing protein 1 2,60E-04 2,61E-04 1,002 -0,106 0,91547385 macrod1 O-acetyl-ADP-ribose deacetylase MACROD1 1,62E-04 1,73E-04 1,064 -0,109 0,91333148 APEH acylamino-acid-releasing enzyme 1,10E-04 1,14E-04 1,037 -0,109 0,91333148 AGPAT3 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma 1,93E-04 2,06E-04 1,067 -0,109 0,91333148 ak7 adenylate kinase 7 7,94E-04 7,68E-04 1,034 -0,109 0,9129885 ACTB actin, cytoplasmic 1 0,001250808 0,001454542 1,163 -0,117 0,90666532 ATP5J2 ATP synthase subunit f, mitochondrial 0,001237756 0,001309186 1,058 -0,118 0,90598778 Arrdc5 arrestin domain-containing protein 5 3,83E-04 4,05E-04 1,058 -0,118 0,90598778 1700020C11Rik mitochondrial fission process protein 1 8,52E-04 8,64E-04 1,014 -0,118 0,90598778 PSMB3 proteasome subunit beta type-3 7,87E-04 8,39E-04 1,067 -0,118 0,90598778 1700080O16Rik uncharacterized protein LOC74279 4,30E-04 4,51E-04 1,050 -0,135 0,89230377 LOC100044248 uncharacterized protein C12orf60 homolog 1,83E-04 2,03E-04 1,113 -0,138 0,88997872 ATP8A2 probable phospholipid-transporting ATPase IB 5,22E-05 5,68E-05 1,088 -0,138 0,88997872

A230051G13Rik putative transferase CAF17 homolog, mitochondrial isoform 1 precursor 1,71E-04 1,90E-04 1,114 -0,138 0,88997872 RAB17 ras-related protein Rab-17 2,28E-04 2,57E-04 1,128 -0,138 0,88997872 WDR65 WD repeat-containing protein 65 4,81E-04 4,88E-04 1,013 -0,152 0,87947051 TMEM146 cation channel sperm-associated protein subunit delta precursor 2,57E-04 2,79E-04 1,086 -0,154 0,87766371 Ak2 adenylate kinase 2, mitochondrial isoform b 0,00192146 0,00185879 1,034 -0,159 0,87382914 gpi1 glucose-6-phosphate isomerase 0,001850572 0,001879843 1,016 -0,162 0,87155781 Lypd4 ly6/PLAUR domain-containing protein 4 precursor 0,002325515 0,002228462 1,044 -0,168 0,86691031 Canx calnexin precursor 4,96E-04 4,76E-04 1,041 -0,173 0,86247835 cyb5r3 NADH-cytochrome b5 reductase 3 1,32E-04 1,53E-04 1,159 -0,174 0,86212605 Prdx6-rs1 peroxiredoxin 6, related sequence 1 1,33E-04 1,46E-04 1,099 -0,174 0,86212605 hprT hypoxanthine-guanine phosphoribosyltransferase 1,63E-04 1,76E-04 1,082 -0,174 0,86212605 PPAP2A lipid phosphate phosphohydrolase 1 isoform 1 6,75E-04 7,39E-04 1,095 -0,174 0,86192002 2810408A11Rik protein phosphatase inhibitor 2-like 3,25E-04 3,68E-04 1,134 -0,174 0,86192002 GSTO2 glutathione S-transferase omega-2 0,001170244 0,001211389 1,035 -0,179 0,85788237 Gm5436 PREDICTED: ATP synthase subunit O, mitochondrial-like 0,002787541 0,002824391 1,013 -0,194 0,84624215 Gm5678 carbonyl reductase [NADPH] 1 2,89E-04 3,33E-04 1,151 -0,196 0,84488939 Gm5347 a disintegrin and metallopeptidase domain 34 2,14E-04 2,29E-04 1,073 -0,196 0,84488939 Arl3 ADP-ribosylation factor-like protein 3 5,12E-04 5,22E-04 1,019 -0,196 0,84488939 PRSS21 testisin precursor 0,003101343 0,00336054 1,084 -0,212 0,83196011 C4bp C4b-binding protein precursor 8,55E-05 9,84E-05 1,151 -0,218 0,82763055 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia ART3 ecto-ADP-ribosyltransferase 3 precursor 1,17E-04 1,38E-04 1,184 -0,218 0,82763055 GCLM glutamate--cysteine ligase regulatory subunit 2,06E-04 2,33E-04 1,134 -0,218 0,82763055 DSTN destrin 1,27E-04 1,34E-04 1,061 -0,218 0,82763055 Ndufa2 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 6,60E-04 6,89E-04 1,044 -0,219 0,82634073 Pdia6 PREDICTED: protein disulfide-isomerase A6 2,75E-04 3,02E-04 1,099 -0,219 0,82634073 LOC675851 NADH dehydrogenase [ubiquinone] 1 subunit C2 0,001257071 0,001451598 1,155 -0,222 0,82458643 HIBCH 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial precursor 3,63E-04 3,93E-04 1,080 -0,222 0,82458643 Nit1 nitrilase homolog 1 isoform 2 8,01E-04 8,73E-04 1,090 -0,232 0,81685575 ftmt ferritin, mitochondrial precursor 6,06E-04 7,18E-04 1,186 -0,240 0,81061739 COX5A cytochrome c oxidase subunit 5A, mitochondrial precursor 0,002934696 0,003036028 1,035 -0,249 0,80363827 EFHC1 EF-hand domain-containing protein 1 8,32E-04 8,43E-04 1,013 -0,267 0,78970792 SLC2A5 solute carrier family 2, facilitated glucose transporter member 5 0,002157979 0,002375321 1,101 -0,268 0,7887294 Actrt2 actin-related protein T2 2,06E-04 2,23E-04 1,081 -0,275 0,78341146 Cfl2 cofilin-2 3,09E-04 3,47E-04 1,122 -0,275 0,78341146 eif5a eukaryotic translation initiation factor 5A-1 2,37E-04 2,50E-04 1,051 -0,276 0,78248223 Krt16 keratin, type I cytoskeletal 16 5,93E-05 7,10E-05 1,198 -0,276 0,78248223 tmed10 transmembrane emp24 domain-containing protein 10 precursor 1,43E-04 1,71E-04 1,195 -0,276 0,78248223 ube4a ubiquitin conjugation factor E4 A 3,91E-05 5,01E-05 1,280 -0,276 0,78248223 Ggct gamma-glutamylcyclotransferase 1,09E-04 1,21E-04 1,110 -0,276 0,78248223 TCEB1 transcription elongation factor B polypeptide 1 2,10E-04 2,75E-04 1,310 -0,276 0,78248223 Rexo2 oligoribonuclease, mitochondrial precursor 9,98E-05 1,15E-04 1,155 -0,276 0,78248223 PLP1 myelin proteolipid protein 1,37E-04 1,70E-04 1,244 -0,276 0,78248223 EFCAB10 EF-hand calcium-binding domain-containing protein 10 2,32E-04 2,57E-04 1,110 -0,276 0,78248223 RNF126 RING finger protein 126 1,56E-04 1,95E-04 1,252 -0,276 0,78248223 PPP4C serine/threonine-protein phosphatase 4 catalytic subunit 7,63E-05 8,99E-05 1,179 -0,276 0,78248223 CCT6B T-complex protein 1 subunit zeta-2 1,20E-04 1,40E-04 1,165 -0,308 0,75812216 1700001C19Rik uncharacterized protein LOC75462 isoform 2 4,69E-04 5,52E-04 1,177 -0,308 0,75812216 1700007G11Rik uncharacterized protein C4orf22 homolog 3,46E-04 4,05E-04 1,170 -0,308 0,75812216 Ccdc116 coiled-coil domain-containing protein 116 1,88E-04 2,20E-04 1,165 -0,308 0,75812216 1700026L06Rik uncharacterized protein C9orf9 homolog 0,001692537 0,001810333 1,070 -0,309 0,75701479 Hyal5 hyaluronidase PH-20 4,88E-04 5,23E-04 1,072 -0,310 0,75632225 APOOL apolipoprotein O-like precursor 3,63E-04 3,92E-04 1,080 -0,324 0,74574041 CYLC2 cylicin, basic protein of sperm head cytoskeleton 2 6,92E-04 7,70E-04 1,113 -0,326 0,74437987 dci enoyl-CoA delta isomerase 1, mitochondrial precursor 6,28E-04 7,02E-04 1,118 -0,328 0,74262813 echdc1 ethylmalonyl-CoA decarboxylase isoform a 1,80E-04 2,25E-04 1,248 -0,346 0,72939199 BPI bactericidal permeability-increasing protein precursor 1,57E-04 1,85E-04 1,179 -0,346 0,72939199 SIRT5 NAD-dependent protein deacylase sirtuin-5, mitochondrial 1,86E-04 2,27E-04 1,223 -0,346 0,72939199 Ptgr2 prostaglandin reductase 2 isoform 1 2,04E-04 2,45E-04 1,199 -0,346 0,72939199 Uchl3 ubiquitin carboxyl-terminal hydrolase isozyme L3 7,56E-04 8,63E-04 1,142 -0,347 0,72829253 APOE apolipoprotein E precursor 3,17E-04 3,68E-04 1,160 -0,350 0,72664142 Ropn1 ropporin-1 0,003890162 0,004150391 1,067 -0,351 0,72592681 sar1a GTP-binding protein SAR1a 8,86E-05 1,18E-04 1,336 -0,361 0,71794509 hspb11 heat shock protein beta-11 1,40E-04 1,95E-04 1,391 -0,361 0,71794509 Xpnpep1 xaa-Pro aminopeptidase 1 2,39E-05 3,34E-05 1,398 -0,361 0,71794509 GDE1 glycerophosphodiester phosphodiesterase 1 6,17E-05 8,23E-05 1,334 -0,361 0,71794509 MYCBPAP MYCBP-associated protein 2,74E-05 3,63E-05 1,325 -0,361 0,71794509 COASY bifunctional coenzyme A synthase precursor 5,06E-05 7,13E-05 1,411 -0,361 0,71794509 2310028O11Rik mitochondrial inner membrane organizing system protein 1 2,43E-04 3,18E-04 1,307 -0,361 0,71794509 pgls 6-phosphogluconolactonase 4,32E-04 4,83E-04 1,120 -0,377 0,70604331 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, Gm9050 mitochondrial-like 8,63E-04 9,72E-04 1,126 -0,377 0,70604331

PPP2CB serine/threonine-protein phosphatase 2A catalytic subunit beta isoform 3,30E-04 3,75E-04 1,135 -0,377 0,70604331 ACAD9 acyl-CoA dehydrogenase family member 9, mitochondrial 4,10E-04 4,54E-04 1,106 -0,380 0,70386834 dihydrolipoyllysine-residue succinyltransferase component of 2- DLST oxoglutarate dehydrogenase complex, mitochondrial 0,001230243 0,0012896 1,048 -0,384 0,70092196 1700061G19Rik uncharacterized protein LOC78625 2,52E-04 2,75E-04 1,089 -0,389 0,69744279 Cox6b2 cytochrome c oxidase subunit 6B2 isoform 2 0,001661682 0,001974845 1,188 -0,389 0,69744279 SPAG6 sperm-associated antigen 6 0,001245622 0,001387715 1,114 -0,399 0,69023425 Hist1h2bp histone H2B type 1-P 4,82E-04 6,08E-04 1,261 -0,407 0,68370192 prmt5 protein arginine N-methyltransferase 5 1,73E-04 2,07E-04 1,194 -0,407 0,68370192 Spaca4 sperm acrosome membrane-associated protein 4 precursor 0,001446927 0,001790601 1,238 -0,411 0,68077798 Gm7204 peroxiredoxin-1 5,67E-04 6,16E-04 1,085 -0,411 0,68077798 MTX2 metaxin-2 0,001073087 0,001188466 1,108 -0,425 0,67083878 psmb1 proteasome subunit beta type-1 precursor 6,58E-04 7,08E-04 1,076 -0,425 0,67083878 Fndc8 fibronectin type III domain-containing protein 8 8,98E-04 0,001022753 1,139 -0,426 0,67045522 1700001C02Rik UPF0573 protein C2orf70 homolog 0,00119726 0,001307938 1,092 -0,426 0,67045522 amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate Als2cr11 11 2,62E-04 3,10E-04 1,182 -0,436 0,66316661 ppp2r4 serine/threonine-protein phosphatase 2A activator 2,50E-04 3,00E-04 1,200 -0,441 0,65931207 PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5- Ndufs5 like 6,30E-04 6,88E-04 1,091 -0,441 0,65931207 csnk1a1 casein kinase I isoform alpha 6,97E-04 7,83E-04 1,123 -0,442 0,65865283 Actc1 actin, alpha cardiac muscle 1 7,98E-05 1,19E-04 1,489 -0,444 0,65701656 LTF lactotransferrin precursor 4,75E-05 6,46E-05 1,358 -0,444 0,65701656 hsd17b4 peroxisomal multifunctional enzyme type 2 5,92E-05 7,86E-05 1,327 -0,444 0,65701656 Arf6 ADP-ribosylation factor 6 2,49E-04 3,17E-04 1,276 -0,444 0,65701656 LOC674583 ATP synthase-coupling factor 6, mitochondrial precursor 2,91E-04 3,62E-04 1,245 -0,444 0,65701656 Dnaic2 dynein intermediate chain 2, axonemal 6,60E-04 7,24E-04 1,096 -0,465 0,64228533 LOC100046510 dynein heavy chain 8, axonemal 4,21E-04 4,30E-04 1,021 -0,474 0,63523292 NDUFB9 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 0,001708703 0,00187838 1,099 -0,475 0,63503671 Arl8b ADP-ribosylation factor-like protein 8B 3,51E-04 4,22E-04 1,205 -0,478 0,63248648 Odf3l1 outer dense fiber protein 3-like protein 1 2,89E-04 3,96E-04 1,373 -0,478 0,63248648 SPACA1 sperm acrosome membrane-associated protein 1 precursor 0,015771523 0,015343843 1,028 -0,479 0,63181937 CS citrate synthase, mitochondrial precursor 0,004193326 0,004347434 1,037 -0,479 0,63164577 Akd1 PREDICTED: adenylate kinase domain-containing protein 1 1,27E-04 1,44E-04 1,134 -0,494 0,62096687 idh3a isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial precursor 0,001500673 0,001611437 1,074 -0,508 0,61147276 ndufa6 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 0,001120881 0,001247727 1,113 -0,510 0,61008676 MOSC2 MOSC domain-containing protein 2, mitochondrial precursor 9,45E-05 1,31E-04 1,381 -0,511 0,60946543 dhodh dihydroorotate dehydrogenase (quinone), mitochondrial precursor 8,98E-05 1,24E-04 1,381 -0,511 0,60946543 Gm16379 macrophage migration inhibitory factor 4,57E-04 6,91E-04 1,513 -0,511 0,60946543 SPINK2 serine protease inhibitor Kazal-type 2 precursor 4,24E-04 5,12E-04 1,206 -0,511 0,60946543 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia Acrv1 acrosomal protein SP-10 precursor 0,005284914 0,005655238 1,070 -0,514 0,60755384 PLCZ1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 1,47E-04 1,85E-04 1,258 -0,520 0,60272523 Phpt1 14 kDa phosphohistidine phosphatase 4,14E-04 5,35E-04 1,293 -0,520 0,60272523 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, NDUFB11 mitochondrial 4,46E-04 5,15E-04 1,155 -0,520 0,60272523 TPPP2 tubulin polymerization-promoting protein family member 2 5,80E-04 6,50E-04 1,120 -0,534 0,59313517 Izumo1 izumo sperm-egg fusion protein 1 precursor 0,003437175 0,0036192 1,053 -0,542 0,58767318 Gm128 protein MENT precursor 0,008122374 0,009205995 1,133 -0,549 0,5830018 glud1 glutamate dehydrogenase 1, mitochondrial precursor 2,25E-04 2,70E-04 1,204 -0,560 0,57534915 Atp5a1 ATP synthase subunit alpha, mitochondrial precursor 0,006271242 0,006031229 1,040 -0,565 0,57174817 PSMD8 26S proteasome non-ATPase regulatory subunit 8 1,84E-04 2,48E-04 1,345 -0,569 0,56937041 ahcY adenosylhomocysteinase 1,22E-04 1,58E-04 1,295 -0,569 0,56937041 ccdc108 coiled-coil domain-containing protein 108 5,02E-05 6,40E-05 1,276 -0,569 0,56937041

Adam24 disintegrin and metalloproteinase domain-containing protein 24 precursor 1,24E-04 1,74E-04 1,400 -0,569 0,56937041 4933405O20Rik probable isocitrate dehydrogenase [NAD] gamma 2, mitochondrial 8,68E-04 9,35E-04 1,078 -0,582 0,56061559 Gm2972 ATP synthase subunit e, mitochondrial 0,001291223 0,001616769 1,252 -0,588 0,55664934 9030607L17Rik izumo sperm-egg fusion protein 4 precursor 0,003361152 0,003770247 1,122 -0,595 0,5517251 LOC100045772 multidrug resistance-associated protein 9 2,47E-05 3,78E-05 1,531 -0,598 0,54955029 Abcg2 ATP-binding cassette sub-family G member 2 6,04E-05 9,47E-05 1,568 -0,598 0,54955029 NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial isoform NDUFV3 2 1,91E-04 2,95E-04 1,543 -0,598 0,54955029 CD36 platelet glycoprotein 4 8,28E-05 1,30E-04 1,574 -0,598 0,54955029 PROM2 prominin-2 isoform 1 precursor 3,67E-05 5,69E-05 1,552 -0,598 0,54955029 NDUFA5 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 0,00148638 0,0016118 1,084 -0,599 0,54898761 Timm44 mitochondrial import inner membrane translocase subunit TIM44 6,09E-04 6,73E-04 1,105 -0,606 0,54471014 Hist1h4c histone H4 8,77E-04 0,001063786 1,213 -0,617 0,53694571 CLTC clathrin heavy chain 1 8,58E-05 1,02E-04 1,190 -0,617 0,53694571 PRDX2 peroxiredoxin-2 6,99E-04 8,54E-04 1,221 -0,624 0,53292262 LRP2BP LRP2-binding protein 1,53E-04 2,12E-04 1,380 -0,626 0,53153718 Tmem11 transmembrane protein 11, mitochondrial isoform 1 3,42E-04 4,67E-04 1,365 -0,626 0,53153718 Styxl1 serine/threonine/tyrosine-interacting-like protein 1 1,63E-04 2,22E-04 1,363 -0,626 0,53153718 - uncharacterized protein LOC73863 4,19E-04 5,35E-04 1,276 -0,626 0,53153718 LYZL1 lysozyme-like protein 1 precursor 4,52E-04 6,98E-04 1,545 -0,626 0,53153718 Gstt4 glutathione S-transferase theta-4 2,17E-04 2,82E-04 1,300 -0,626 0,53153718 PPIL6 peptidyl-prolyl cis-trans isomerase-like 6 3,81E-04 4,77E-04 1,251 -0,628 0,53000279 LOC674469 ATP synthase subunit d, mitochondrial 0,002673368 0,002979167 1,114 -0,638 0,52323027 iars2 isoleucine--tRNA ligase, mitochondrial precursor 7,97E-04 8,76E-04 1,100 -0,657 0,51103177 methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial Aldh6a1 precursor 3,67E-04 4,47E-04 1,218 -0,658 0,51029079 4930579C15Rik equatorin precursor 0,002434144 0,002625419 1,079 -0,672 0,501887 lancl1 lanC-like protein 1 2,47E-04 3,19E-04 1,295 -0,672 0,50133691 WDR64 WD repeat-containing protein 64 1,46E-04 1,89E-04 1,295 -0,676 0,49878999 FAM154A uncharacterized protein LOC75811 9,52E-04 0,001100669 1,156 -0,679 0,49716113 APOA1 apolipoprotein A-I preproprotein 3,57E-04 4,65E-04 1,301 -0,684 0,49410237 HSPA9 stress-70 protein, mitochondrial 0,001724229 0,001867697 1,083 -0,690 0,49037418 LTA4H leukotriene A-4 hydrolase 7,20E-05 1,07E-04 1,483 -0,693 0,48800643 SSNA1 Sjoegren syndrome nuclear autoantigen 1 homolog 2,93E-04 4,31E-04 1,474 -0,693 0,48800643 1700080E11Rik uncharacterized protein LOC73532 1,72E-04 2,53E-04 1,471 -0,693 0,48800643

PPP3CC serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform 3,26E-04 4,25E-04 1,302 -0,705 0,48062017 arsA arylsulfatase A precursor 4,23E-04 5,60E-04 1,324 -0,721 0,4707118 pcmt1 protein-L-isoaspartate(D-aspartate) O-methyltransferase 2,43E-04 3,31E-04 1,365 -0,722 0,47000524 slc16a7 monocarboxylate transporter 2 4,95E-04 6,44E-04 1,301 -0,722 0,47000524 BPHL valacyclovir hydrolase precursor 4,78E-05 8,85E-05 1,850 -0,723 0,46996472 atp6v0d1 V-type proton ATPase subunit d 1 5,06E-05 9,71E-05 1,918 -0,723 0,46996472 COQ10A coenzyme Q-binding protein COQ10 homolog A, mitochondrial 5,75E-05 1,09E-04 1,893 -0,723 0,46996472 KRT6A keratin, type II cytoskeletal 6A 3,65E-05 7,21E-05 1,972 -0,723 0,46996472 NUDT2 bis(5-nucleosyl)-tetraphosphatase [asymmetrical] 9,21E-05 1,84E-04 1,998 -0,723 0,46996472 NUDCD2 nudC domain-containing protein 2 1,17E-04 2,01E-04 1,716 -0,723 0,46996472 Ubl4 ubiquitin-like protein 4A 8,93E-05 1,61E-04 1,798 -0,723 0,46996472 RAB22A ras-related protein Rab-22A 6,36E-05 1,15E-04 1,801 -0,723 0,46996472 Gm7684 E3 ubiquitin-protein ligase MARCH5 isoform 1 5,81E-05 1,11E-04 1,912 -0,723 0,46996472 1700024G13Rik UPF0728 protein C10orf53 homolog 1,65E-04 3,11E-04 1,882 -0,723 0,46996472 Cdc42 cell division control protein 42 homolog isoform 1 precursor 8,29E-05 1,57E-04 1,890 -0,723 0,46996472 #N/A hemoglobin subunit beta-1 0,002431798 0,002838928 1,167 -0,728 0,46678649 complement component 1 Q subcomponent-binding protein, c1qbp mitochondrial 8,29E-04 0,001037886 1,251 -0,731 0,46501306 TEPP testis, prostate and placenta-expressed protein isoform 1 0,001329739 0,001739518 1,308 -0,738 0,4603308 DNALI1 axonemal dynein light intermediate polypeptide 1 0,002106784 0,002325986 1,104 -0,756 0,44986134 coiled-coil-helix-coiled-coil-helix domain-containing protein 3, CHCHD3 mitochondrial precursor 0,001079432 0,001231988 1,141 -0,756 0,4497832 Gm2962 cytochrome b-c1 complex subunit 7 0,001044334 0,001257562 1,204 -0,757 0,44889363 cct5 T-complex protein 1 subunit epsilon 3,02E-04 3,65E-04 1,206 -0,757 0,44889363 ropn1l ropporin-1-like protein 8,28E-04 9,82E-04 1,187 -0,757 0,44882818 CISD1 CDGSH iron-sulfur domain-containing protein 1 0,00186544 0,001923299 1,031 -0,757 0,44882818 Gm9234 peptidyl-prolyl cis-trans isomerase A 4,30E-04 5,73E-04 1,334 -0,762 0,44581079 Gm5562 glutathione S-transferase Mu 1 3,09E-04 4,14E-04 1,337 -0,762 0,44581079 SPAG16 sperm-associated antigen 16 protein isoform 1 1,66E-04 2,26E-04 1,363 -0,762 0,44581079 PHOSPHO1 phosphoethanolamine/phosphocholine phosphatase 8,46E-04 0,001029244 1,216 -0,764 0,44499598 TSNAXIP1 translin-associated factor X-interacting protein 1 1,66E-04 2,10E-04 1,267 -0,769 0,44177996 Psma4 proteasome subunit alpha type-4 4,75E-04 6,22E-04 1,308 -0,769 0,44177996 ppiB peptidyl-prolyl cis-trans isomerase B precursor 4,23E-04 5,71E-04 1,348 -0,769 0,44177996 cct6a T-complex protein 1 subunit zeta 5,27E-04 6,18E-04 1,172 -0,774 0,43905147 GOT1 aspartate aminotransferase, cytoplasmic 6,63E-05 1,10E-04 1,653 -0,777 0,43705504 Pdilt protein disulfide-isomerase-like protein of the testis precursor 5,62E-05 9,05E-05 1,611 -0,777 0,43705504 Tmem89 transmembrane protein 89 precursor 4,04E-04 6,91E-04 1,708 -0,777 0,43705504 Gm14316 serine protease inhibitor, Kunitz type 5 precursor 2,88E-04 4,54E-04 1,576 -0,777 0,43705504 DNAJB3 dnaJ homolog subfamily B member 3 2,29E-04 3,43E-04 1,501 -0,780 0,43553885 eIF6 eukaryotic translation initiation factor 6 3,66E-04 5,42E-04 1,480 -0,780 0,43553885 nsdhl sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating 2,13E-04 3,12E-04 1,468 -0,780 0,43553885 man2c1 alpha-mannosidase 2C1 2,11E-04 2,70E-04 1,281 -0,785 0,43221102 4732415M23Rik uncharacterized protein C16orf55 homolog 6,20E-04 8,65E-04 1,396 -0,808 0,41922412 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia CCDC135 coiled-coil domain-containing protein lobo homolog 2,20E-04 2,77E-04 1,260 -0,832 0,40541654 Gm1060 dynein regulatory complex protein 1 2,46E-04 3,14E-04 1,278 -0,832 0,40541654 Gm5928 ubiquitin-40S ribosomal protein S27a precursor 0,003155673 0,003394076 1,076 -0,835 0,40367549 4921511H03Rik uncharacterized protein C7orf62 homolog 5,32E-04 7,26E-04 1,366 -0,843 0,39914803 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial Gm12251 precursor 0,002238367 0,00263213 1,176 -0,846 0,39738967 PSMA1 proteasome subunit alpha type-1 9,15E-04 0,00110216 1,204 -0,852 0,39429232 MORN3 MORN repeat-containing protein 3 2,47E-04 3,83E-04 1,552 -0,858 0,39075234 SPACA5 sperm acrosome-associated protein 5 precursor 5,62E-04 7,73E-04 1,375 -0,858 0,39075234 Uba1 ubiquitin-like modifier-activating enzyme 1 isoform 2 2,12E-04 2,69E-04 1,267 -0,859 0,39013117 NCSTN nicastrin precursor 3,42E-04 4,54E-04 1,328 -0,859 0,39013117 DYDC1 DPY30 domain-containing protein 1 8,81E-04 0,001103999 1,253 -0,859 0,39013117 ENKUR enkurin 9,85E-04 0,001294439 1,315 -0,876 0,38105295 ACTL7A actin-like protein 7A 9,45E-04 0,001120965 1,186 -0,882 0,37775 Mdh1 malate dehydrogenase, cytoplasmic 2,87E-04 4,03E-04 1,405 -0,885 0,37621647 TXNDC8 thioredoxin domain-containing protein 8 1,44E-04 2,51E-04 1,743 -0,885 0,37619065 4930412F15Rik protein FAM221B 6,28E-05 1,17E-04 1,868 -0,885 0,37619065 hadh hydroxyacyl-coenzyme A dehydrogenase, mitochondrial precursor 9,47E-05 1,74E-04 1,836 -0,885 0,37619065 NIPSNAP1 protein NipSnap homolog 1 8,65E-05 1,67E-04 1,932 -0,885 0,37619065 G-protein coupled receptor family C group 5 member B isoform 2 Gprc5b precursor 1,85E-04 3,39E-04 1,831 -0,885 0,37619065 NDUFAB1 acyl carrier protein, mitochondrial precursor 2,02E-04 3,44E-04 1,703 -0,885 0,37619065 csnk2a2 casein kinase II subunit alpha 0,001812506 0,001957684 1,080 -0,898 0,36894126 1700007K13Rik UPF0691 protein C9orf116 homolog 5,49E-04 8,24E-04 1,500 -0,915 0,36017072 LYZL4 lysozyme-like protein 4 precursor 4,89E-04 7,35E-04 1,504 -0,915 0,36017072 YWHAE 14-3-3 protein epsilon 2,09E-04 3,02E-04 1,442 -0,915 0,36017072 LOC100044101 thioredoxin reductase 2, mitochondrial precursor 9,84E-05 1,67E-04 1,693 -0,925 0,35474782 4930578I06Rik uncharacterized protein C8orf74 homolog 1,59E-04 2,66E-04 1,675 -0,925 0,35474782 psmc4 26S protease regulatory subunit 6B 1,13E-04 2,00E-04 1,778 -0,925 0,35474782 Dguok deoxyguanosine kinase, mitochondrial isoform 1 1,62E-04 2,68E-04 1,654 -0,925 0,35474782 CCDC113 coiled-coil domain-containing protein 113 9,72E-05 1,58E-04 1,625 -0,925 0,35474782 NRD1 nardilysin precursor 4,66E-05 7,49E-05 1,607 -0,925 0,35474782 cetn1 centrin-1 2,23E-04 3,49E-04 1,564 -0,925 0,35474782 APOA1BP NAD(P)H-hydrate epimerase precursor 6,23E-04 8,32E-04 1,336 -0,951 0,34159883 LOC100046684 PREDICTED: ES1 protein homolog, mitochondrial-like isoform 1 4,03E-04 5,58E-04 1,384 -0,956 0,33916581 wbp2nl postacrosomal sheath WW domain-binding protein 8,00E-04 0,001077479 1,346 -0,956 0,33892053 Hspa1l heat shock 70 kDa protein 1-like 0,001118638 0,001316742 1,177 -0,961 0,33668522 PEBP1 phosphatidylethanolamine-binding protein 1 0,004571822 0,005565085 1,217 -0,964 0,33526426 Prkaca cAMP-dependent protein kinase catalytic subunit alpha isoform 2 0,001111348 0,001283776 1,155 -0,964 0,3351608 - regulator of G-protein signaling 22 5,39E-05 8,41E-05 1,559 -0,980 0,32723161 COX7A2 cytochrome c oxidase subunit 7A2, mitochondrial precursor 7,14E-04 0,001138431 1,596 -0,980 0,32723161 NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial NDUFS6 precursor 3,75E-04 5,84E-04 1,558 -0,980 0,32723161 Lcn5 epididymal-specific lipocalin-5 isoform a precursor 4,46E-04 6,25E-04 1,401 -0,981 0,32669093 mycophenolic acid acyl-glucuronide esterase, mitochondrial isoform 1 ABHD10 precursor 7,12E-04 9,62E-04 1,351 -0,981 0,32639009 ATP1A1 sodium/potassium-transporting ATPase subunit alpha-1 precursor 3,63E-05 6,88E-05 1,897 -1,022 0,30685028 ACSL1 long-chain-fatty-acid--CoA ligase 1 0,002157522 0,002365198 1,096 -1,029 0,30325287 LOC674211 14-3-3 protein theta 6,27E-05 1,43E-04 2,281 -1,033 0,30178822 GPX1 glutathione peroxidase 1 7,16E-05 1,73E-04 2,413 -1,033 0,30178822 prkcsh glucosidase 2 subunit beta precursor 5,68E-05 1,31E-04 2,308 -1,033 0,30178822 tmem38b trimeric intracellular cation channel type B 8,01E-05 1,72E-04 2,151 -1,033 0,30178822 Gm5867 UMP-CMP kinase 6,69E-05 1,45E-04 2,170 -1,033 0,30178822 Prdx5 peroxiredoxin-5, mitochondrial precursor 0,001178548 0,001449037 1,230 -1,034 0,30136171 PTCHD3 patched domain-containing protein 3 8,90E-04 0,001054104 1,184 -1,035 0,30055813 BC061194 uncharacterized protein LOC381350 2,33E-04 3,58E-04 1,534 -1,043 0,29679266 LOC100048601 probable threonine protease PRSS50 precursor 3,73E-04 5,62E-04 1,509 -1,043 0,29679266 LOC100048867 annexin A2 2,24E-04 3,24E-04 1,450 -1,043 0,29679266 NDUFAF2 mimitin, mitochondrial 1,92E-04 3,57E-04 1,860 -1,054 0,29167438 psmc3 26S protease regulatory subunit 6A 9,67E-05 1,64E-04 1,697 -1,054 0,29167438 INSL6 insulin-like peptide INSL6 precursor 2,52E-04 4,61E-04 1,828 -1,054 0,29167438 pfkp 6-phosphofructokinase type C 0,001375013 0,001564629 1,138 -1,060 0,28892605 Acr acrosin isoform 3 0,002649188 0,003184981 1,202 -1,066 0,28622907

Ndufa13 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 0,001882751 0,002366145 1,257 -1,071 0,28408133 nagK N-acetyl-D-glucosamine kinase isoform 1 5,50E-04 7,32E-04 1,331 -1,084 0,27843145 CCDC63 coiled-coil domain-containing protein 63 9,73E-04 0,001122433 1,154 -1,091 0,2753315 Gm711 probable inactive protein kinase-like protein SgK071 homolog 1,77E-04 2,79E-04 1,580 -1,099 0,27169968 succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial LOC638710 precursor 0,001428904 0,001645641 1,152 -1,108 0,26793817 NHEDC1 sodium/hydrogen exchanger 9B1 5,87E-04 8,02E-04 1,366 -1,108 0,26777255 TTC18 tetratricopeptide repeat domain 18 isoform 3 1,67E-04 2,43E-04 1,451 -1,121 0,26248458 pgam1 phosphoglycerate mutase 1 1,75E-04 3,07E-04 1,755 -1,142 0,25325246 NUBPL iron-sulfur protein NUBPL precursor 1,45E-04 2,75E-04 1,894 -1,142 0,25325246 Uchl1 ubiquitin carboxyl-terminal hydrolase isozyme L1 2,08E-04 3,75E-04 1,800 -1,142 0,25325246 HSPA4 heat shock 70 kDa protein 4 2,80E-05 6,02E-05 2,151 -1,144 0,25270774 Gm10078 28S ribosomal protein S36, mitochondrial isoform 1 4,27E-04 8,29E-04 1,941 -1,144 0,25270774 rdh11 retinol dehydrogenase 11 precursor 9,39E-05 1,90E-04 2,026 -1,144 0,25270774 ACAA2 3-ketoacyl-CoA thiolase, mitochondrial 6,50E-05 1,44E-04 2,208 -1,144 0,25270774 ACTL9 actin-like protein 9 9,35E-05 2,03E-04 2,173 -1,144 0,25270774 rab5b ras-related protein Rab-5B isoform 1 1,13E-04 2,56E-04 2,263 -1,144 0,25270774 RALA ras-related protein Ral-A precursor 1,20E-04 2,49E-04 2,078 -1,144 0,25270774 Wfdc15b WAP four-disulfide core domain protein 15B precursor 2,60E-04 5,36E-04 2,058 -1,144 0,25270774 CCDC39 coiled-coil domain-containing protein 39 2,56E-04 3,16E-04 1,235 -1,144 0,2525417 1700023E05Rik testicular haploid expressed gene protein-like 1,59E-04 2,58E-04 1,625 -1,148 0,25110898 Lrrc18 leucine-rich repeat-containing protein 18 isoform 1 3,36E-04 4,95E-04 1,473 -1,148 0,25094989 SLC8A2 sodium/calcium exchanger 2 precursor 2,02E-04 2,88E-04 1,428 -1,148 0,25094989 LOC100048880 AFG3-like protein 2 1,47E-04 2,09E-04 1,421 -1,160 0,2462137 Psma6 proteasome subunit alpha type-6 7,84E-04 0,001059576 1,352 -1,165 0,24406681 LETM1 and EF-hand domain-containing protein 1, mitochondrial LETM1 precursor 3,67E-04 4,59E-04 1,251 -1,168 0,24270085 Ado 2-aminoethanethiol dioxygenase 2,23E-04 4,32E-04 1,938 -1,170 0,24197482 LdhA L-lactate dehydrogenase A chain isoform 2 0,001834479 0,00222475 1,213 -1,192 0,23318971 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia REEP6 receptor expression-enhancing protein 6 isoform 1 6,05E-04 0,001041048 1,721 -1,208 0,22700137 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial SDHA precursor 0,002819026 0,003153249 1,119 -1,240 0,21495462 TCTE1 T-complex-associated testis-expressed protein 1 8,71E-05 1,71E-04 1,965 -1,249 0,21179858 PGD 6-phosphogluconate dehydrogenase, decarboxylating 7,39E-05 1,45E-04 1,968 -1,249 0,21179858 LOC630729 glutathione reductase, mitochondrial precursor 8,14E-05 1,72E-04 2,115 -1,249 0,21179858 Gm7655 isopentenyl-diphosphate Delta-isomerase 1 1,20E-04 2,31E-04 1,931 -1,249 0,21179858 NDUFA3 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 4,52E-04 0,001001684 2,214 -1,249 0,21179858 IMPA1 inositol monophosphatase 1 8,96E-04 0,001171277 1,308 -1,271 0,20374086 TTC19 tetratricopeptide repeat protein 19, mitochondrial isoform 1 2,03E-04 3,49E-04 1,720 -1,276 0,20208124 PSMD13 26S proteasome non-ATPase regulatory subunit 13 1,61E-04 2,71E-04 1,687 -1,276 0,20208124 PRKAR2A cAMP-dependent protein kinase type II-alpha regulatory subunit 0,003106839 0,003556261 1,145 -1,276 0,20200972 CCT8 T-complex protein 1 subunit theta 5,62E-04 6,84E-04 1,217 -1,294 0,19559816 4933430H15Rik leucine-rich repeat-containing protein 71 4,30E-05 1,17E-04 2,728 -1,306 0,19151923 1700009P17Rik UPF0740 protein C1orf192 homolog 7,93E-04 0,001509581 1,903 -1,309 0,19058927 PLBD2 putative phospholipase B-like 2 precursor 2,00E-04 3,39E-04 1,698 -1,309 0,19058927 disintegrin and metalloproteinase domain-containing protein 5 isoform 1 Adam5 precursor 1,06E-04 1,70E-04 1,605 -1,309 0,19058927 Gm11273 cytochrome c oxidase subunit 5B, mitochondrial 0,001782416 0,002139059 1,200 -1,321 0,18652639 PITRM1 presequence protease, mitochondrial precursor 3,34E-04 4,37E-04 1,307 -1,322 0,18613988 Sod2 superoxide dismutase [Mn], mitochondrial precursor 0,001323928 0,001912007 1,444 -1,324 0,18551085 Gm4045 ATP synthase subunit g, mitochondrial 0,001321867 0,001990348 1,506 -1,338 0,18077688 RAB11B ras-related protein Rab-11B 0,001850543 0,002351272 1,271 -1,357 0,17492992 CSNK2B casein kinase II subunit beta 0,00130153 0,001834047 1,409 -1,365 0,17239315 Gm10108 cytochrome c, somatic 5,17E-04 8,99E-04 1,737 -1,373 0,16963433 Mdh1b putative malate dehydrogenase 1B 6,52E-05 1,56E-04 2,387 -1,381 0,16737638 UGP2 UTP--glucose-1-phosphate uridylyltransferase 4,64E-05 1,14E-04 2,446 -1,381 0,16737638 LOC674674 farnesyl pyrophosphate synthase isoform 2 8,50E-05 2,13E-04 2,511 -1,381 0,16737638 EFCAB5 EF-hand calcium-binding domain-containing protein 5 6,97E-05 1,15E-04 1,653 -1,403 0,16065453 Ywhaz 14-3-3 protein zeta/delta isoform 1 0,001708833 0,002339608 1,369 -1,422 0,15515512 Dnalc1 dynein light chain 1, axonemal 6,84E-04 9,90E-04 1,448 -1,426 0,15397317 CLMN calmin isoform a 1,56E-04 2,44E-04 1,564 -1,426 0,1539168 Prss39 inactive serine protease 39 precursor 0,001312689 0,001858602 1,416 -1,438 0,1504084 3110056O03Rik signal peptide peptidase-like 2B precursor 1,52E-04 3,36E-04 2,213 -1,439 0,15017175 CCDC96 coiled-coil domain-containing protein 96 8,16E-05 1,60E-04 1,956 -1,439 0,15017175 st13 hsc70-interacting protein 1,56E-04 3,16E-04 2,031 -1,439 0,15017175 UCHL5 ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 1 1,71E-04 3,42E-04 1,995 -1,439 0,15017175 Dynlt1b dynein light chain Tctex-type 1F isoform 1 5,68E-04 0,001162115 2,048 -1,439 0,15017175 NUP54 nuclear pore complex protein Nup54 1,08E-04 2,23E-04 2,071 -1,439 0,15017175 4932425I24Rik protein MAATS1 9,18E-05 1,72E-04 1,871 -1,445 0,14839861 4921507P07Rik uncharacterized protein C7orf31 homolog 1,51E-04 2,91E-04 1,921 -1,445 0,14839861 vapa vesicle-associated membrane protein-associated protein A 3,22E-04 5,76E-04 1,787 -1,445 0,14839861 bsg basigin isoform 2 precursor 0,003476643 0,004154273 1,195 -1,453 0,14621708 Adam3 a disintegrin and metalloprotease domain 3 (cyritestin) precursor 5,31E-05 1,19E-04 2,235 -1,460 0,14418567 Gm5429 uncharacterized protein C7orf72 homolog 2,76E-04 7,27E-04 2,638 -1,460 0,14418567 GALC galactocerebrosidase precursor 8,83E-05 2,07E-04 2,339 -1,460 0,14418567 Dnahc7a dynein, axonemal, heavy chain 7B 4,64E-05 6,81E-05 1,469 -1,468 0,14203243 Sypl synaptophysin-like protein 1 isoform 1 7,93E-04 0,001463156 1,846 -1,472 0,14095514 Fbp1 fructose-1,6-bisphosphatase 1 9,06E-04 0,001268184 1,399 -1,490 0,13624382 CASC1 cancer susceptibility candidate protein 1 1,47E-04 2,45E-04 1,674 -1,491 0,13586699 Cyct cytochrome c, testis-specific 8,20E-04 0,001337479 1,631 -1,522 0,12804035 AA467197 normal mucosa of esophagus-specific gene 1 protein 0,004325182 0,005404045 1,249 -1,533 0,12523608 uqcrfs1 cytochrome b-c1 complex subunit Rieske, mitochondrial 0,002996527 0,003718043 1,241 -1,539 0,12375927 tomm40 mitochondrial import receptor subunit TOM40 homolog 1,68E-04 3,76E-04 2,238 -1,540 0,12357688 IQCD IQ domain-containing protein D 7,24E-05 1,63E-04 2,258 -1,540 0,12357688 Pdzk1 Na(+)/H(+) exchange regulatory cofactor NHE-RF3 6,31E-05 1,38E-04 2,181 -1,540 0,12357688 GLOD4 glyoxalase domain-containing protein 4 1,10E-04 2,37E-04 2,161 -1,540 0,12357688 HSP90AB1 heat shock protein HSP 90-beta 5,53E-05 1,17E-04 2,111 -1,540 0,12357688 Gbas protein NipSnap homolog 2 2,00E-04 4,41E-04 2,208 -1,540 0,12357688

- PREDICTED: up-regulated during skeletal muscle growth protein 5-like 6,89E-04 0,001582726 2,296 -1,540 0,12357688 succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial SUCLA2 precursor 0,004455064 0,005042605 1,132 -1,542 0,12314279 succinyl-CoA:3-ketoacid coenzyme A transferase 2B, mitochondrial LOC100046138 precursor 0,007713328 0,008633791 1,119 -1,549 0,12132808 Nup53 nucleoporin NUP53 isoform 2 4,13E-04 7,74E-04 1,873 -1,551 0,12092616 LOC677611 casein kinase II subunit alpha 3,21E-04 5,21E-04 1,625 -1,551 0,12092616 SLC3A2 4F2 cell-surface antigen heavy chain isoform a 3,54E-05 1,14E-04 3,234 -1,552 0,12063055 SCP2 non-specific lipid-transfer protein 3,55E-05 1,12E-04 3,151 -1,552 0,12063055 4930579J09Rik protein phosphatase 1 regulatory subunit 32 2,92E-04 5,18E-04 1,774 -1,555 0,12003562 HSD17B10 3-hydroxyacyl-CoA dehydrogenase type-2 7,00E-04 0,001071851 1,530 -1,560 0,11877296 CALR calreticulin precursor 7,20E-05 2,00E-04 2,777 -1,600 0,10967163 STUB1 STIP1 homology and U box-containing protein 1 5,98E-05 1,64E-04 2,748 -1,600 0,10967163 iscA2 iron-sulfur cluster assembly 2 homolog, mitochondrial precursor 1,05E-04 2,90E-04 2,759 -1,600 0,10967163 GHITM growth hormone-inducible transmembrane protein 1,23E-04 3,17E-04 2,569 -1,600 0,10967163 NIPSNAP3B protein NipSnap homolog 3B 2,98E-04 6,19E-04 2,075 -1,608 0,10773215 CCDC42 coiled-coil domain-containing protein 42A 2,35E-04 4,34E-04 1,845 -1,616 0,10612749 snap23 synaptosomal-associated protein 23 isoform a 1,81E-04 3,94E-04 2,179 -1,618 0,10573601 PPT1 palmitoyl-protein thioesterase 1 precursor 2,20E-04 4,50E-04 2,044 -1,618 0,10573601 1700101E01Rik uncharacterized protein LOC329375 isoform 1 5,52E-04 9,18E-04 1,663 -1,623 0,10453191 TTC25 tetratricopeptide repeat protein 25 4,32E-04 6,00E-04 1,389 -1,652 0,09854133 Atp6v1a V-type proton ATPase catalytic subunit A 1,94E-04 3,39E-04 1,747 -1,655 0,09794528 DNAJC10 dnaJ homolog subfamily C member 10 precursor 4,54E-05 1,20E-04 2,633 -1,659 0,09705356 - uncharacterized protein LOC69439 4,53E-05 1,12E-04 2,475 -1,659 0,09705356 EFCAB1 EF-hand calcium-binding domain-containing protein 1 1,48E-04 3,48E-04 2,356 -1,659 0,09705356 CCDC105 coiled-coil domain-containing protein 105 0,001150891 0,001457893 1,267 -1,662 0,09655752 RSPH6A radial spoke head protein 6 homolog A isoform 1 0,001399858 0,001883812 1,346 -1,667 0,09544306 stom erythrocyte band 7 integral membrane protein 0,001611922 0,002095574 1,300 -1,668 0,0953162

HAGH hydroxyacylglutathione hydrolase, mitochondrial isoform 1 precursor 2,06E-04 4,19E-04 2,037 -1,693 0,09044879 LOC631229 ADP/ATP translocase 2 2,41E-04 4,30E-04 1,782 -1,695 0,09012453 Fam71e2 uncharacterized protein LOC243822 1,62E-04 2,96E-04 1,830 -1,695 0,09012453 LAP3 cytosol aminopeptidase 0,001376879 0,001729692 1,256 -1,695 0,09007918 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia SYNGR4 synaptogyrin-4 0,003253489 0,004416072 1,357 -1,704 0,0883896 psmA2 proteasome subunit alpha type-2 5,99E-04 0,001051914 1,756 -1,710 0,08719147 PSMD1 26S proteasome non-ATPase regulatory subunit 1 2,61E-04 4,01E-04 1,535 -1,728 0,08399864 Fhl4 four and a half LIM domains 4 6,69E-04 9,82E-04 1,468 -1,730 0,08362736 putative ATP-dependent Clp protease proteolytic subunit, mitochondrial clpP precursor 7,39E-04 0,001231198 1,665 -1,730 0,08362736 Cpt1b carnitine O-palmitoyltransferase 1, muscle isoform 3,92E-04 5,77E-04 1,472 -1,730 0,08357231 cyc1 cytochrome c1, heme protein, mitochondrial 0,002751021 0,003728242 1,355 -1,732 0,08327946 Gk2 glycerol kinase 2 0,001312167 0,00170677 1,301 -1,749 0,08035987 PSMA8 proteasome subunit alpha type-7-like 7,01E-04 0,001073877 1,532 -1,763 0,07790676 CSNK1G2 casein kinase I isoform gamma-2 isoform 1 1,75E-04 3,92E-04 2,241 -1,766 0,0774028 Prdx6 peroxiredoxin-6 3,24E-04 6,07E-04 1,877 -1,770 0,07670588 1700011E24Rik uncharacterized protein C2orf61 homolog 5,61E-05 2,17E-04 3,876 -1,777 0,0756081 SLC13A5 solute carrier family 13 member 5 6,58E-05 2,23E-04 3,394 -1,777 0,0756081 NME2 nucleoside diphosphate kinase B 1,10E-04 4,23E-04 3,845 -1,777 0,0756081 Sort1 sortilin isoform 2 precursor 2,65E-05 9,56E-05 3,603 -1,777 0,0756081 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, NDUFA10 mitochondrial precursor 0,001176298 0,001667841 1,418 -1,786 0,0740897 PGK2 phosphoglycerate kinase 2 0,003359474 0,003822795 1,138 -1,792 0,07313562 4931432M23Rik inactive serine protease 54 precursor 9,55E-05 3,17E-04 3,322 -1,804 0,0712647 A330021E22Rik uncharacterized protein C7orf63 homolog 3,11E-05 9,34E-05 2,999 -1,804 0,0712647 Adgb androglobin 1,79E-05 5,44E-05 3,038 -1,804 0,0712647 LOC100048051 multidrug and toxin extrusion protein 2 9,60E-05 2,95E-04 3,077 -1,804 0,0712647

Cox7a2l cytochrome c oxidase subunit 7A-related protein, mitochondrial isoform 1 2,07E-04 7,31E-04 3,525 -1,804 0,0712647 Rab14 ras-related protein Rab-14 0,002840961 0,003648892 1,284 -1,805 0,07107169 Fahd2a fumarylacetoacetate hydrolase domain-containing protein 2A 0,001935646 0,00253361 1,309 -1,816 0,06931164 Gm11582 dynein light chain 1, cytoplasmic 0,001096984 0,002058239 1,876 -1,835 0,06655388 Adam6a a disintegrin and metalloprotease domain 6 4,40E-04 6,75E-04 1,533 -1,836 0,06642509 THOP1 thimet oligopeptidase 4,87E-05 1,31E-04 2,689 -1,847 0,06472106 Wdr52 WD repeat-containing protein 52 2,74E-04 3,95E-04 1,443 -1,851 0,06419478 GRPEL1 grpE protein homolog 1, mitochondrial precursor 2,50E-04 5,39E-04 2,152 -1,854 0,06376553 Gm2903 10 kDa heat shock protein, mitochondrial 2,66E-04 6,05E-04 2,274 -1,854 0,06376553 SLC44A5 choline transporter-like protein 5 4,84E-04 7,75E-04 1,602 -1,862 0,06262063 RAB4B ras-related protein Rab-4B 4,80E-04 8,32E-04 1,734 -1,875 0,0608445 POLDIP2 polymerase delta-interacting protein 2 1,07E-04 2,89E-04 2,705 -1,898 0,057677 Usp7 ubiquitin carboxyl-terminal hydrolase 7 1,93E-04 3,04E-04 1,571 -1,904 0,05687888 Slc25a11 mitochondrial 2-oxoglutarate/malate carrier protein 2,26E-04 4,74E-04 2,096 -1,905 0,05680934 Tmem177 transmembrane protein 177 2,44E-04 5,88E-04 2,409 -1,918 0,05509269 ARMC3 armadillo repeat-containing protein 3 isoform 1 1,89E-04 3,17E-04 1,676 -1,919 0,05500456 STX12 syntaxin-12 6,13E-04 0,001150961 1,878 -1,925 0,05418658 Tssk2 testis-specific serine/threonine-protein kinase 2 1,23E-04 2,90E-04 2,356 -1,953 0,05085574 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, NDUFA9 mitochondrial precursor 8,51E-04 0,00126258 1,484 -1,962 0,0497142 HSP90AA1 heat shock protein HSP 90-alpha 4,67E-04 6,69E-04 1,432 -1,962 0,0497142 PGAM2 phosphoglycerate mutase 2 0,004041497 0,004839845 1,198 -1,965 0,04940877 1700028P14Rik uncharacterized protein C9orf135 homolog 2,68E-04 5,71E-04 2,132 -1,971 0,04873023 wdr16 WD repeat-containing protein 16 8,44E-04 0,001169221 1,385 -1,975 0,04826592 LOC675857 transitional endoplasmic reticulum ATPase 0,001181458 0,001488977 1,260 -1,977 0,04808819 camk4 calcium/calmodulin-dependent protein kinase type IV 4,35E-05 1,82E-04 4,181 -1,984 0,04723459 VDAC1 voltage-dependent anion-selective channel protein 1 3,91E-04 7,06E-04 1,806 -1,992 0,04638265 ak3 GTP:AMP phosphotransferase AK3, mitochondrial 8,09E-05 2,83E-04 3,494 -1,995 0,04600574 npepps puromycin-sensitive aminopeptidase 3,00E-05 1,02E-04 3,413 -1,995 0,04600574 dcaf7 DDB1- and CUL4-associated factor 7 8,55E-05 2,97E-04 3,474 -1,995 0,04600574 Ace3 angiotensin I converting enzyme 3 precursor 9,22E-04 0,001278842 1,387 -2,008 0,04465881 Ogdh 2-oxoglutarate dehydrogenase, mitochondrial isoform 1 0,0018471 0,002125695 1,151 -2,020 0,04341108 Stoml2 stomatin-like protein 2, mitochondrial 9,08E-05 2,66E-04 2,936 -2,025 0,0428254 RAB28 ras-related protein Rab-28 1,16E-04 3,65E-04 3,150 -2,025 0,0428254 1700023D19Rik izumo sperm-egg fusion protein 2 precursor 6,86E-04 0,001288857 1,878 -2,036 0,04170815 HYDIN hydrocephalus-inducing protein 3,75E-05 6,73E-05 1,794 -2,056 0,03975231 BPGM bisphosphoglycerate mutase 6,12E-04 0,001048901 1,714 -2,056 0,03975231 Hba-a1 hemoglobin alpha, adult chain 2 0,002400706 0,003868159 1,611 -2,082 0,0373791 sorD sorbitol dehydrogenase 0,003430472 0,004406884 1,285 -2,135 0,03276923 #N/A PREDICTED: leucine-rich repeat-containing protein 37A2 5,73E-04 7,78E-04 1,358 -2,137 0,03259101 ATP8B3 probable phospholipid-transporting ATPase IK 2,98E-04 4,58E-04 1,538 -2,159 0,0308668 Dnahc2 dynein heavy chain 2, axonemal 3,72E-05 6,92E-05 1,863 -2,168 0,03014636 Ogdhl 2-oxoglutarate dehydrogenase-like, mitochondrial 2,65E-05 9,99E-05 3,771 -2,176 0,02954534 SSSCA1 Sjoegren syndrome/scleroderma autoantigen 1 homolog 1,63E-04 6,13E-04 3,759 -2,176 0,02954534 YWHAB 14-3-3 protein beta/alpha 6,81E-05 3,14E-04 4,609 -2,177 0,02944416 Tmprss12 transmembrane protease serine 12 precursor 6,78E-05 3,07E-04 4,527 -2,177 0,02944416 GLIPR1L2 GLIPR1-like protein 2 7,43E-04 0,001418883 1,909 -2,179 0,02929501 Man2b1 lysosomal alpha-mannosidase precursor 4,57E-05 1,54E-04 3,377 -2,195 0,02815273 HTRA2 serine protease HTRA2, mitochondrial 5,88E-04 0,001048764 1,784 -2,211 0,02704559 ACOT7 cytosolic acyl coenzyme A thioester hydrolase isoform 2 1,34E-04 3,96E-04 2,962 -2,225 0,02604976 Acsl6 long-chain-fatty-acid--CoA ligase 6 isoform 1 5,88E-05 1,82E-04 3,093 -2,225 0,02604976 RAB2A ras-related protein Rab-2A 0,008902561 0,010802675 1,213 -2,227 0,02596504 4932412H11Rik leucine-rich repeat and death domain-containing protein 1 1,30E-04 2,82E-04 2,172 -2,233 0,02552494 Dnahc6 axonemal dynein heavy chain 9,39E-05 1,44E-04 1,535 -2,241 0,02505443 Dynll2 dynein light chain 2, cytoplasmic 8,76E-04 0,00204365 2,334 -2,259 0,02390504 psmd7 26S proteasome non-ATPase regulatory subunit 7 1,64E-04 4,74E-04 2,890 -2,262 0,02367144 succinyl-CoA:3-ketoacid coenzyme A transferase 2A, mitochondrial Oxct2a precursor 0,00775306 0,009015523 1,163 -2,273 0,02303609 psmd3 26S proteasome non-ATPase regulatory subunit 3 9,75E-05 2,63E-04 2,702 -2,303 0,02125709 PSMD2 26S proteasome non-ATPase regulatory subunit 2 4,17E-04 6,68E-04 1,602 -2,325 0,02005319 psma5 proteasome subunit alpha type-5 6,14E-04 0,001203565 1,959 -2,333 0,01964491 hscB iron-sulfur cluster co-chaperone protein HscB, mitochondrial precursor 2,21E-04 5,55E-04 2,506 -2,347 0,01893521 1700029J07Rik UPF0602 protein C4orf47 homolog 2,18E-04 5,59E-04 2,565 -2,347 0,01893521 odf3 outer dense fiber protein 3 0,002903542 0,003834831 1,321 -2,347 0,01893185 #N/A insulin-degrading enzyme 2,66E-05 1,08E-04 4,052 -2,348 0,01889504 xpo1 exportin-1 2,11E-05 1,07E-04 5,074 -2,359 0,01832727 Fahd1 acylpyruvase FAHD1, mitochondrial 1,98E-04 6,04E-04 3,047 -2,379 0,01733783 glb1l beta-galactosidase-1-like protein precursor 7,09E-05 2,34E-04 3,304 -2,379 0,01733783 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus musculus Mus spicilegus APEX Z-score p presenta mayor abundancia Ndufa7 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 4,32E-04 0,001048272 2,428 -2,392 0,01677087 Cd46 membrane cofactor protein precursor 4,98E-04 0,001011859 2,031 -2,395 0,01663825 Ccdc19 nasopharyngeal epithelium specific protein 1 3,62E-04 6,29E-04 1,735 -2,395 0,01660266 Fam82b regulator of microtubule dynamics protein 1 1,69E-04 4,80E-04 2,844 -2,409 0,01598871 Wdr96 WD repeat domain 96 9,96E-05 2,05E-04 2,060 -2,441 0,01466254 Abca17 ATP-binding cassette, sub-family A (ABC1), member 17 5,36E-05 1,43E-04 2,661 -2,444 0,01453569 Crat carnitine O-acetyltransferase 0,003778255 0,004857197 1,286 -2,457 0,01402931 XPO7 exportin-7 1,47E-04 3,07E-04 2,086 -2,498 0,01249711 slc25a10 mitochondrial dicarboxylate carrier 8,60E-04 0,001473962 1,715 -2,501 0,01238437 high affinity cAMP-specific and IBMX-insensitive 3,5-cyclic PDE8A phosphodiesterase 8A 3,51E-05 1,53E-04 4,347 -2,511 0,01204288 EFHB EF-hand domain-containing family member B 1,24E-04 2,84E-04 2,297 -2,530 0,01141183 Fam71a uncharacterized protein LOC619288 5,77E-04 9,54E-04 1,652 -2,534 0,01128071 ALDH2 aldehyde dehydrogenase, mitochondrial precursor 1,14E-04 3,36E-04 2,961 -2,580 0,0098816 Dnah7 PREDICTED: dynein heavy chain 7, axonemal 3,02E-05 6,68E-05 2,213 -2,622 0,00874089 ak1 adenylate kinase isoenzyme 1 isoform 1 0,00200719 0,003045266 1,517 -2,682 0,00732736 ech1 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial precursor 5,05E-05 2,79E-04 5,511 -2,693 0,00708215 MYCBP C-Myc-binding protein 4,56E-04 0,001381946 3,028 -2,712 0,00668216 Csl citrate synthase-like protein 0,004375624 0,005620394 1,284 -2,751 0,005941 SPAG17 sperm-associated antigen 17 8,19E-05 1,74E-04 2,120 -2,758 0,005816 Wdr78 WD repeat-containing protein 78 9,89E-05 2,84E-04 2,866 -2,771 0,00558734 DNAJB13 dnaJ homolog subfamily B member 13 6,97E-04 0,001218112 1,748 -2,800 0,00511627 DPP3 dipeptidyl peptidase 3 4,68E-05 1,95E-04 4,167 -2,806 0,00501244 1700093K21Rik uncharacterized protein C2orf74 homolog 1,65E-04 8,15E-04 4,935 -2,817 0,00485373 mmel1 membrane metallo-endopeptidase-like 1 7,61E-05 2,55E-04 3,347 -2,822 0,00477181 Atp5c1 ATP synthase subunit gamma, mitochondrial isoform a 0,001863501 0,00279509 1,500 -2,904 0,00368557 ATP1A4 sodium/potassium-transporting ATPase subunit alpha-4 0,001962105 0,002639071 1,345 -2,917 0,00353972 HSPA5 78 kDa glucose-regulated protein precursor 3,68E-04 6,71E-04 1,825 -2,930 0,0033889 Akr1b3 aldose reductase 1,47E-04 5,33E-04 3,633 -2,944 0,00324319 Gnpda2 glucosamine-6-phosphate isomerase 2 1,36E-04 5,27E-04 3,888 -2,944 0,00324319 GNPAT dihydroxyacetone phosphate acyltransferase 4,85E-05 2,16E-04 4,458 -2,945 0,00322739 LOC630540 WD repeat domain 63 2,40E-04 5,42E-04 2,258 -3,081 0,00206515 PARK7 protein DJ-1 6,28E-04 0,001475203 2,349 -3,159 0,00158318 Hsp90b1 endoplasmin precursor 3,10E-04 5,80E-04 1,869 -3,178 0,00148466 ARMC4 armadillo repeat-containing protein 4 4,80E-04 7,83E-04 1,631 -3,194 0,00140411 PREDICTED: orphan sodium- and chloride-dependent neurotransmitter Ntt5 transporter NTT5 1,15E-04 5,79E-04 5,053 -3,210 0,00132792 Ttc39d tetratricopeptide repeat domain 39D 1,31E-04 4,45E-04 3,404 -3,221 0,00127884

Gm4335 PREDICTED: glyceraldehyde-3-phosphate dehydrogenase-like isoform 2 0,001520003 0,002434633 1,602 -3,223 0,00126761 Pdia4 protein disulfide-isomerase A4 precursor 3,50E-05 2,10E-04 5,987 -3,233 0,00122497 dhrs1 dehydrogenase/reductase SDR family member 1 2,27E-04 6,99E-04 3,080 -3,258 0,0011236 aga N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase isoform 1 precursor 6,08E-05 4,65E-04 7,645 -3,276 0,00105362 lrrc23 leucine-rich repeat-containing protein 23 7,27E-05 4,38E-04 6,028 -3,363 7,72E-04 TUBB2C tubulin beta-4B chain 0,003313337 0,004455719 1,345 -3,416 6,35E-04 ATP5B ATP synthase subunit beta, mitochondrial precursor 0,00822562 0,010215866 1,242 -3,529 4,18E-04 EFCAB6 EF-hand calcium-binding domain-containing protein 6 isoform 1 3,53E-04 5,86E-04 1,662 -3,562 3,68E-04 Heatr7b2 maestro heat-like repeat-containing protein family member 2B 3,91E-04 6,36E-04 1,627 -3,573 3,53E-04 EFR3A protein EFR3 homolog A 4,21E-05 2,45E-04 5,828 -3,579 3,45E-04 fsip2 PREDICTED: fibrous sheath-interacting protein 2 1,53E-04 2,40E-04 1,562 -3,615 3,01E-04 ACRBP acrosin-binding protein isoform 1 precursor 0,001834115 0,002872778 1,566 -3,635 2,78E-04 Ldhc L-lactate dehydrogenase C chain 0,010370016 0,012658673 1,221 -3,699 2,16E-04 bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase dak (cyclizing) 6,69E-04 0,001351786 2,022 -3,706 2,10E-04 BC049730 uncharacterized protein LOC232972 precursor 1,57E-04 0,001111177 7,098 -3,729 1,92E-04 Acox3 peroxisomal acyl-coenzyme A oxidase 3 9,63E-05 3,79E-04 3,934 -3,765 1,66E-04 LOC100039128 nuclear pore complex protein Nup93 1,19E-04 3,71E-04 3,112 -3,819 1,34E-04 succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial suclg1 precursor 0,006051398 0,00893407 1,476 -3,886 1,02E-04 Dnahc1 dynein, axonemal, heavy chain 1 6,36E-05 1,49E-04 2,334 -4,019 5,84E-05 Dnhc12 PREDICTED: dynein heavy chain 12, axonemal 5,66E-05 1,37E-04 2,417 -4,041 5,33E-05 Tabla suplementaria 14. Análisis comparativos entre Mus spretus y Mus spicilegus. Las proteínas con diferencias significativas son indicadas en negrita.

Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia AKAP4 A-kinase anchor protein 4 isoform b 0,005202227 0,002431018 2,139937504 10,60407472 0 Mus spretus trifunctional enzyme subunit alpha, mitochondrial HADHA precursor 0,002128039 6,70E-04 3,174478175 8,955922721 0 Mus spretus trifunctional enzyme subunit beta, mitochondrial Gm13910 precursor 0,003116362 7,62E-04 4,089220695 8,259184635 2,22E-16 Mus spretus sorD sorbitol dehydrogenase 0,008220805 0,004406884 1,865446062 7,076884863 1,47E-12 Mus spretus HK1 hexokinase-1 isoform HK1-sb 0,005196722 0,003929661 1,322435328 6,176670398 6,55E-10 Mus spretus Gm7172 tubulin alpha-1A chain 8,76E-04 1,80E-04 4,863597276 6,104039879 1,03E-09 Mus spretus Hbb hemoglobin, beta adult s chain 0,004506586 0,001704711 2,643606727 5,893881773 3,77E-09 Mus spretus cat eye syndrome critical region protein 5 homolog CECR5 precursor 0,00142685 3,27E-04 4,360809122 5,558646232 2,72E-08 Mus spretus cytochrome b-c1 complex subunit 1, mitochondrial Uqcrc1 precursor 0,00527534 0,002899343 1,819494841 5,158091697 2,49E-07 Mus spretus SQRDL sulfide:quinone oxidoreductase, mitochondrial 8,45E-04 2,40E-04 3,51786704 4,973070909 6,59E-07 Mus spretus L-2-hydroxyglutarate dehydrogenase, mitochondrial L2HGDH precursor 0,00153357 6,61E-04 2,32144189 4,946460697 7,56E-07 Mus spretus Odf2 outer dense fiber protein 2 isoform e 0,004393161 0,003166166 1,387533047 4,905805494 9,30E-07 Mus spretus NT5C1B cytosolic 5-nucleotidase 1B 0,004628911 0,002939427 1,574766602 4,718100108 2,38E-06 Mus spretus tekt3 tektin-3 0,002396197 0,001317636 1,818558196 4,619662462 3,84E-06 Mus spretus

GAPDHS glyceraldehyde-3-phosphate dehydrogenase, testis-specific 0,007178342 0,005258094 1,365198523 4,537070974 5,70E-06 Mus spretus cAMP-dependent protein kinase type II-alpha regulatory PRKAR2A subunit 0,005355296 0,003556261 1,505878167 4,532346301 5,83E-06 Mus spretus PGK2 phosphoglycerate kinase 2 0,00600817 0,003822795 1,571669372 4,514295753 6,35E-06 Mus spretus Gm12141 60 kDa heat shock protein, mitochondrial 0,003763253 0,002833138 1,328298522 4,334582099 1,46E-05 Mus spretus succinyl-CoA ligase [ADP-forming] subunit beta, SUCLA2 mitochondrial precursor 0,006505705 0,005042605 1,290147682 4,272539217 1,93E-05 Mus spretus glycerol-3-phosphate dehydrogenase, mitochondrial Gpd2 precursor 0,001780131 0,001190297 1,495534458 4,107601299 4,00E-05 Mus spretus Spnb1 spectrin beta chain, erythrocytic 1,11E-05 8,00E-05 7,235321199 -4,317230248 1,58E-05 Mus spicilegus monofunctional C1-tetrahydrofolate synthase, MTHFD1L mitochondrial precursor 1,36E-04 3,91E-04 2,874051051 -4,376096826 1,21E-05 Mus spicilegus dhrs1 dehydrogenase/reductase SDR family member 1 1,38E-04 6,99E-04 5,052084817 -4,457441252 8,29E-06 Mus spicilegus FABP9 fatty acid-binding protein 9 0,00166101 0,004057311 2,442677173 -4,969832155 6,70E-07 Mus spicilegus Hba-a1 hemoglobin alpha, adult chain 2 0,001116662 0,003868159 3,464037348 -5,310480762 1,09E-07 Mus spicilegus XPO7 exportin-7 3,13E-05 3,07E-04 9,780443645 -5,407675834 6,38E-08 Mus spicilegus Hsp90b1 endoplasmin precursor 1,64E-04 5,80E-04 3,538253112 -5,467819188 4,56E-08 Mus spicilegus Adam6a a disintegrin and metalloprotease domain 6 1,43E-04 6,75E-04 4,708641742 -5,632082674 1,78E-08 Mus spicilegus MME neprilysin 3,04E-05 4,82E-04 15,82200331 -6,271854172 3,57E-10 Mus spicilegus MUC5B mucin 5, subtype B, tracheobronchial precursor 5,70E-06 1,40E-04 24,6217181 -6,567228855 5,13E-11 Mus spicilegus Pcx pyruvate carboxylase, mitochondrial isoform 2 3,06E-05 3,94E-04 12,87804624 -7,26705314 3,67E-13 Mus spicilegus atp2b4 plasma membrane calcium ATPase 4 isoform a 1,43E-04 0,001385159 9,715745814 -11,61908282 3,30E-31 Mus spicilegus FCGBP Fc fragment of IgG binding protein-like precursor 2,49E-05 7,72E-04 31,02346132 -13,06098151 5,50E-39 Mus spicilegus Pkm pyruvate kinase, muscle isoform M1 0,006761807 0,005653915 1,195951271 3,932036519 8,42E-05 tekt1 tektin-1 0,001421598 6,57E-04 2,163480677 3,908553307 9,29E-05 serine/threonine-protein phosphatase PGAM5, mitochondrial pgam5 isoform 2 0,00118842 4,29E-04 2,77164767 3,835947643 1,25E-04 GLOD4 glyoxalase domain-containing protein 4 7,70E-04 2,37E-04 3,249573674 3,788747756 1,51E-04 man2c1 alpha-mannosidase 2C1 6,38E-04 2,70E-04 2,359476693 3,741352386 1,83E-04 spata18 mitochondria-eating protein 0,002173621 0,001297617 1,675086281 3,736098659 1,87E-04 dihydrolipoyllysine-residue acetyltransferase component of dlaT pyruvate dehydrogenase complex, mitochondrial 0,005934628 0,004652064 1,27569774 3,727820052 1,93E-04 LOC100046792 uncharacterized protein C6orf163 homolog 5,26E-04 1,33E-04 3,967586999 3,56849071 3,59E-04 FSCB fibrous sheath CABYR-binding protein 0,00234733 0,001454292 1,614070899 3,492602628 4,78E-04 CRISP1 cysteine-rich secretory protein 1 precursor 8,62E-04 2,01E-04 4,281676452 3,465796328 5,29E-04 MPI mannose-6-phosphate isomerase 5,29E-04 1,14E-04 4,651474563 3,363052743 7,71E-04 INPP5A inositol polyphosphate-5-phosphatase A isoform b 4,18E-04 1,04E-04 4,028631577 3,352894282 8,00E-04 acot9 acyl-coenzyme A thioesterase 9, mitochondrial 0,002592449 0,001704036 1,521358356 3,341925288 8,32E-04 IL4I1 L-amino-acid oxidase precursor 0,001965055 0,001201265 1,635820971 3,337284567 8,46E-04 Ppp1r7 protein phosphatase 1 regulatory subunit 7 3,86E-04 7,86E-05 4,903096318 3,261720127 0,00110738 cpvl probable serine carboxypeptidase CPVL precursor 5,55E-04 1,50E-04 3,710537875 3,237559337 0,00120557 H2-Ke6 estradiol 17-beta-dehydrogenase 8 0,002737824 0,001476132 1,854728772 3,217828576 0,00129165 CCT2 T-complex protein 1 subunit beta 0,001026517 5,81E-04 1,767511978 3,191265948 0,00141651 D-beta-hydroxybutyrate dehydrogenase, mitochondrial BDH1 precursor 0,001483235 7,86E-04 1,888033358 3,146074727 0,00165478 TEKT4 tektin-4 8,78E-04 4,55E-04 1,932071522 3,144840252 0,00166178 G6pdx glucose-6-phosphate 1-dehydrogenase X 4,27E-04 1,33E-04 3,198135982 3,14443541 0,00166408 GLUL glutamine synthetase 0,001431574 6,48E-04 2,207842846 3,127122706 0,00176526 phosphatidylethanolamine-binding protein 4 isoform a Pebp4 precursor 0,001779406 7,31E-04 2,432570217 3,12072076 0,00180409 NADH dehydrogenase [ubiquinone] flavoprotein 1, Ndufv1 mitochondrial precursor 0,001594127 9,46E-04 1,685878304 3,095481243 0,00196494 gpi1 glucose-6-phosphate isomerase 0,002862517 0,001879843 1,522743114 3,038661695 0,00237632 cpt2 carnitine O-palmitoyltransferase 2, mitochondrial precursor 9,52E-04 5,11E-04 1,86069307 2,99609482 0,00273461 2810408A11Rik protein phosphatase inhibitor 2-like 9,38E-04 3,68E-04 2,546877421 2,978326146 0,00289827 CYLC1 cylicin-1 4,48E-04 1,87E-04 2,398340451 2,946242501 0,0032166 bifunctional ATP-dependent dihydroxyacetone kinase/FAD- dak AMP lyase (cyclizing) 0,001997272 0,001351786 1,477505716 2,926195448 0,00343135 Echs1 enoyl-CoA hydratase, mitochondrial precursor 5,58E-04 1,55E-04 3,591193762 2,872536289 0,00407191 Ropn1 ropporin-1 0,005343302 0,004150391 1,287421395 2,817591504 0,00483853 1190002A17Rik adenylate kinase 8 0,001003532 6,24E-04 1,607294018 2,799392003 0,00511989 Sept7 septin-7 isoform 1 5,79E-04 2,69E-04 2,153824421 2,747623965 0,00600288

Uqcrc2 cytochrome b-c1 complex subunit 2, mitochondrial precursor 0,007346425 0,005865026 1,252581811 2,733821935 0,00626039

SLC2A3 solute carrier family 2, facilitated glucose transporter member 3 0,004831955 0,003357845 1,439004903 2,717333506 0,00658103 Igbp1b immunoglobulin-binding protein 1b 0,001211404 5,92E-04 2,044887525 2,706722846 0,0067951 cbr4 carbonyl reductase family member 4 0,001357221 6,66E-04 2,036425215 2,706722846 0,0067951 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia CCIN calicin 0,001413515 9,07E-04 1,558954164 2,687741859 0,0071937 Aldoa1b aldolase 1 A retrogene 2 0,001391983 7,62E-04 1,825956323 2,672315238 0,00753298 DKKL1 dickkopf-like protein 1 precursor 3,49E-04 5,86E-05 5,950986386 2,636454609 0,00837774 Tuba3a tubulin alpha-3 chain 0,002186908 0,00171741 1,273375816 2,618690138 0,00882681 ACAD9 acyl-CoA dehydrogenase family member 9, mitochondrial 7,42E-04 4,54E-04 1,634692347 2,57224699 0,01010408 Adam6b a disintegrin and metalloproteinase domain 6-like 6,53E-04 3,48E-04 1,876522363 2,568236831 0,01022173 Psmd11 26S proteasome non-ATPase regulatory subunit 11 5,73E-04 2,69E-04 2,127108336 2,542870695 0,01099459 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase PLCD4 delta-4 isoform 1 3,36E-04 1,23E-04 2,7309757 2,529065396 0,01143667 CREG1 protein CREG1 precursor 9,26E-04 3,19E-04 2,908206985 2,529065396 0,01143667 - UPF0704 protein C6orf165 homolog 3,05E-04 1,03E-04 2,97053078 2,476026066 0,01328539 Aldoa fructose-bisphosphate aldolase A isoform 1 precursor 0,001656066 0,001089093 1,520592589 2,425147403 0,01530217 Psmc6 26S protease regulatory subunit 10B 3,61E-04 1,34E-04 2,691819613 2,39927748 0,01642746 WDR65 WD repeat-containing protein 65 7,05E-04 4,88E-04 1,444852373 2,384262822 0,01711338 oplaH 5-oxoprolinase 1,56E-04 5,54E-05 2,8134208 2,365678262 0,01799708 hsd17b4 peroxisomal multifunctional enzyme type 2 2,12E-04 7,86E-05 2,704920211 2,365678262 0,01799708 methylmalonate-semialdehyde dehydrogenase [acylating], Aldh6a1 mitochondrial precursor 7,57E-04 4,47E-04 1,692135856 2,360436177 0,01825346 TEKT2 tektin-2 0,001364028 9,09E-04 1,499979565 2,351924405 0,01867657 Sept10 septin-10 isoform 1 4,35E-04 1,74E-04 2,506280876 2,348658929 0,01884116 TAGLN transgelin 7,58E-04 3,09E-04 2,451858446 2,348658929 0,01884116 4930511I11Rik armadillo repeat-containing protein 12 0,001218772 5,55E-04 2,19518122 2,331500501 0,01972698 succinyl-CoA:3-ketoacid coenzyme A transferase 2B, LOC100046138 mitochondrial precursor 0,009599666 0,008633791 1,111871377 2,327262415 0,0199513 complement decay-accelerating factor, GPI-anchored Cd55 precursor 7,86E-04 3,43E-04 2,292385428 2,315327713 0,020595 Angel1 protein angel homolog 2 isoform 2 2,66E-04 8,28E-05 3,209795711 2,310419514 0,02086494 cryz quinone oxidoreductase 0,001038221 5,71E-04 1,818329301 2,289565269 0,02204653 phosphatidylglycerophosphatase and protein-tyrosine ptpmt1 phosphatase 1 7,08E-04 3,30E-04 2,149883057 2,244478839 0,02480161 mitochondrial import inner membrane translocase subunit Timm44 TIM44 0,00103557 6,73E-04 1,538638146 2,226222814 0,02599926 Rnls renalase isoform 1 0,004596706 0,00329343 1,395720072 2,22248784 0,02625035 Spesp1 sperm equatorial segment protein 1 precursor 0,009194719 0,007310148 1,257801989 2,220185415 0,02640618 ZPBP2 zona pellucida-binding protein 2 isoform 1 precursor 0,003500504 0,002693968 1,299386257 2,200662689 0,02775991 Prdx6-rs1 peroxiredoxin 6, related sequence 1 4,33E-04 1,46E-04 2,960996967 2,190150091 0,02851335 Fndc8 fibronectin type III domain-containing protein 8 0,001672348 0,001022753 1,635143416 2,184151251 0,02895114 TEKT5 tektin-5 0,001379043 9,40E-04 1,467804372 2,170468142 0,0299714 trim69 E3 ubiquitin-protein ligase TRIM69 5,00E-04 2,66E-04 1,877393054 2,167904535 0,03016595 CS citrate synthase, mitochondrial precursor 0,005511836 0,004347434 1,26783657 2,163215685 0,03052459 Apoh beta-2-glycoprotein 1 precursor 3,01E-04 9,95E-05 3,025434904 2,157945387 0,03093208 Fh1 fumarate hydratase, mitochondrial precursor 8,15E-04 4,77E-04 1,70962122 2,154369906 0,03121117 cAMP-dependent protein kinase catalytic subunit alpha isoform Prkaca 2 0,001867731 0,001283776 1,45487272 2,148246205 0,0316942 electron transfer flavoprotein-ubiquinone oxidoreductase, Etfdh mitochondrial precursor 1,65E-04 5,16E-05 3,194227784 2,132733096 0,03294663 CPA5 carboxypeptidase A5 precursor 4,81E-04 1,95E-04 2,464047037 2,128579947 0,03328903 Serpinb6a serpin B6 isoform a 1,79E-04 3,77E-05 4,756123429 2,126796061 0,03343703 NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, ndufs2 mitochondrial precursor 0,001556297 0,0010109 1,539515859 2,122967995 0,03375653 serine beta-lactamase-like protein LACTB, mitochondrial LOC677144 precursor 1,57E-04 4,35E-05 3,603131089 2,119274989 0,03406723 NUBPL iron-sulfur protein NUBPL precursor 5,90E-04 2,75E-04 2,146356982 2,091604079 0,03647395 KDM2A lysine-specific demethylase 2A 1,37E-04 6,01E-05 2,284019647 2,039495167 0,04140063 PSMD12 26S proteasome non-ATPase regulatory subunit 12 2,99E-04 1,37E-04 2,184252772 2,039495167 0,04140063 Iqcg IQ domain-containing protein G 4,58E-04 2,20E-04 2,07791666 2,014786993 0,04392697 Hdhd1a pseudouridine-5-monophosphatase 8,11E-04 3,24E-04 2,503504429 1,99928565 0,04557746 fucA1 tissue alpha-L-fucosidase precursor 3,84E-04 1,35E-04 2,845289767 1,99928565 0,04557746 pyruvate dehydrogenase E1 component subunit alpha, somatic Pdha1 form, mitochondrial precursor 4,75E-04 2,52E-04 1,883142597 1,998636956 0,04564765 UGP2 UTP--glucose-1-phosphate uridylyltransferase 2,59E-04 1,14E-04 2,282521593 1,987117727 0,04690935 Cd46 membrane cofactor protein precursor 0,001608731 0,001011859 1,58987729 1,969267843 0,04892234 Oit1 protein FAM3D precursor 3,21E-04 9,47E-05 3,393724635 1,940338411 0,05233858 Qsox1 sulfhydryl oxidase 1 isoform b precursor 1,15E-04 2,83E-05 4,051354592 1,93558835 0,05291814 GLIPR1L2 GLIPR1-like protein 2 0,002062109 0,001418883 1,453333062 1,922734369 0,05451342 succinyl-CoA:3-ketoacid coenzyme A transferase 1, oxct1 mitochondrial precursor 4,31E-04 2,49E-04 1,732255601 1,886671983 0,05920446 Prdx5 peroxiredoxin-5, mitochondrial precursor 0,002102354 0,001449037 1,450862497 1,882437025 0,0597767 Atp5a1 ATP synthase subunit alpha, mitochondrial precursor 0,007283678 0,006031229 1,207660547 1,867455789 0,06183797 SPAG6 sperm-associated antigen 6 0,001875728 0,001387715 1,351666348 1,86608009 0,06203016 Tuba8 tubulin alpha-8 chain 9,66E-04 7,24E-04 1,335290439 1,848075714 0,06459139 LOC100045796 cysteine desulfurase, mitochondrial 6,96E-04 4,71E-04 1,479355331 1,819780685 0,06879241

#N/A PREDICTED: leucine-rich repeat-containing protein 37A2 9,76E-04 7,78E-04 1,253616365 1,818959708 0,06891758 ME1 NADP-dependent malic enzyme isoform 1 7,52E-04 4,99E-04 1,505314322 1,813597478 0,06973972 LOC100045163 phospholipase B-like 1 precursor 7,88E-04 5,09E-04 1,547434868 1,794140743 0,07279071 mdh2 malate dehydrogenase, mitochondrial precursor 0,004574706 0,003779323 1,210456567 1,794030794 0,07280825

PPP1CB serine/threonine-protein phosphatase PP1-beta catalytic subunit 2,62E-04 1,04E-04 2,52348042 1,788180838 0,07374684 aldoa1a aldolase 1 A retrogene 1 6,18E-04 3,55E-04 1,738424257 1,768712762 0,07694182 HINT1 histidine triad nucleotide-binding protein 1 5,69E-04 1,78E-04 3,198957048 1,750704691 0,07999679 SDR39U1 epimerase family protein SDR39U1 0,001403069 8,53E-04 1,64432525 1,732579158 0,0831705 pnpla8 calcium-independent phospholipase A2-gamma 7,39E-05 1,96E-05 3,770620953 1,730338921 0,08356974 Lrrc52 leucine-rich repeat-containing protein 52 precursor 3,18E-04 9,33E-05 3,413123504 1,730338921 0,08356974 Gm1096 PREDICTED: CD177 antigen isoform 2 5,11E-04 2,14E-04 2,387946136 1,720836141 0,08528056 Hyal5 hyaluronidase PH-20 8,37E-04 5,23E-04 1,600250959 1,705719489 0,08806032 dld dihydrolipoyl dehydrogenase, mitochondrial precursor 0,003469746 0,002677128 1,296070425 1,700432176 0,08904966 LOC677611 casein kinase II subunit alpha 8,70E-04 5,21E-04 1,669182727 1,695363135 0,09000655 bat3 large proline-rich protein BAG6 isoform 1 7,69E-04 5,45E-04 1,410110263 1,692955211 0,09046399 PPM1A protein phosphatase 1A 3,15E-04 1,49E-04 2,105034544 1,690332005 0,09096446 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia Aldoa2 fructose-bisphosphate aldolase A isoform 2 0,001438588 0,001000885 1,437315334 1,665586038 0,09579589 DPP4 dipeptidyl peptidase 4 isoform 2 1,42E-04 5,79E-05 2,447551151 1,660608002 0,0967922 ALDH9A1 4-trimethylaminobutyraldehyde dehydrogenase precursor 3,97E-04 2,45E-04 1,620256448 1,651907881 0,09855333 Arpm1 actin-related protein T3 6,16E-04 3,64E-04 1,694880933 1,633478226 0,10236846 npepps puromycin-sensitive aminopeptidase 1,85E-04 1,02E-04 1,80250898 1,609330277 0,10754414 Erlin2 erlin-2 8,29E-04 5,48E-04 1,513167726 1,603895452 0,1087371 CTBP2 C-terminal-binding protein 2 isoform 1 1,10E-04 4,35E-05 2,519547313 1,602095369 0,10913452 pdcd6 programmed cell death protein 6 4,39E-04 1,75E-04 2,508660302 1,602095369 0,10913452 ivd isovaleryl-CoA dehydrogenase, mitochondrial precursor 1,84E-04 7,08E-05 2,601035509 1,602095369 0,10913452 ACAT1 acetyl-CoA acetyltransferase, mitochondrial precursor 0,001027675 6,82E-04 1,506460625 1,599847418 0,10963244 1700071K01Rik prohibitin 0,002595415 0,002115717 1,226731033 1,589850598 0,11186849 EFHC1 EF-hand domain-containing protein 1 0,001108667 8,43E-04 1,314798383 1,585986412 0,11274239 sccpdh saccharopine dehydrogenase-like oxidoreductase 0,003928832 0,00315341 1,245899544 1,582669373 0,11349684 Gm5347 a disintegrin and metallopeptidase domain 34 4,12E-04 2,29E-04 1,799695464 1,560711105 0,11859193 ATP6V1E2 V-type proton ATPase subunit E 2 3,65E-04 1,60E-04 2,288434008 1,551384125 0,12080966 Cops8 COP9 signalosome complex subunit 8 4,56E-04 1,74E-04 2,625448906 1,54858006 0,1214827 DNPEP aspartyl aminopeptidase isoform b 0,002349307 0,002109802 1,113519834 1,536920481 0,12431278 prcp lysosomal Pro-X carboxypeptidase precursor 6,44E-04 4,19E-04 1,535093515 1,531108536 0,12574257

PPIF peptidyl-prolyl cis-trans isomerase F, mitochondrial precursor 1,96E-04 6,24E-05 3,148329872 1,508004365 0,13155341 STARD6 stAR-related lipid transfer protein 6 1,99E-04 6,34E-05 3,141799958 1,508004365 0,13155341

IDH2 isocitrate dehydrogenase [NADP], mitochondrial precursor 9,77E-05 3,06E-05 3,191381532 1,508004365 0,13155341 Stoml2 stomatin-like protein 2, mitochondrial 4,67E-04 2,66E-04 1,751701179 1,48455554 0,1376616 Akr7a5 aflatoxin B1 aldehyde reductase member 2 8,56E-04 5,31E-04 1,613892836 1,484201039 0,13775559 KCNU1 potassium channel subfamily U member 1 1,33E-04 6,23E-05 2,136159205 1,478865765 0,13917619 Gm216 uncharacterized protein C2orf62 homolog 2,61E-04 1,18E-04 2,220913991 1,478865765 0,13917619 Acad10 acyl-CoA dehydrogenase family member 10 1,02E-04 5,24E-05 1,936815459 1,456296551 0,14531066 GOT1 aspartate aminotransferase, cytoplasmic 2,13E-04 1,10E-04 1,945136919 1,456296551 0,14531066 psmb2 proteasome subunit beta type-2 9,36E-04 6,42E-04 1,459423516 1,455713173 0,14547193

Gm13570 PREDICTED: mitochondrial pyruvate carrier-like protein-like 0,001715179 9,77E-04 1,755992621 1,455713173 0,14547193 psmc4 26S protease regulatory subunit 6B 3,52E-04 2,00E-04 1,75772112 1,45565174 0,14548892 Csl citrate synthase-like protein 0,006476897 0,005620394 1,152392034 1,443574132 0,14885881 Lpcat2b lysophosphatidylcholine acyltransferase 2B 1,47E-04 6,25E-05 2,352853714 1,405009236 0,16001853 Gm614 uncharacterized protein CXorf65 homolog 5,73E-04 3,14E-04 1,825081129 1,384623605 0,16616759 pyruvate dehydrogenase E1 component subunit alpha, testis- pdhA2 specific form, mitochondrial precursor 0,007200256 0,006577202 1,094729359 1,384457598 0,16621838 CCDC81 coiled-coil domain-containing protein 81 2,54E-04 1,60E-04 1,586874711 1,376748349 0,16859006

Carkd ATP-dependent (S)-NAD(P)H-hydrate dehydratase isoform 1 3,14E-04 1,76E-04 1,782022057 1,372381699 0,16994464 pfkp 6-phosphofructokinase type C 0,001817018 0,001564629 1,16130884 1,353372752 0,17593658 SPATC1 speriolin 0,001259147 8,83E-04 1,425824923 1,35194029 0,17639442 st13 hsc70-interacting protein 5,44E-04 3,16E-04 1,718734857 1,347043122 0,17796635 - PREDICTED: uncharacterized protein LOC77908 0,001835138 0,001119488 1,639264944 1,347043122 0,17796635 Gm5081 ribose-phosphate pyrophosphokinase 1 1,80E-04 7,62E-05 2,364125002 1,331664356 0,18297051 Xpnpep1 xaa-Pro aminopeptidase 1 8,14E-05 3,34E-05 2,438749663 1,331664356 0,18297051 4921507P07Rik uncharacterized protein C7orf31 homolog 4,52E-04 2,91E-04 1,555897963 1,327977919 0,1841854 Pgk1-rs7 phosphoglycerate kinase 1 5,47E-04 3,70E-04 1,478962255 1,323251576 0,18575173 BCS1L mitochondrial chaperone BCS1 3,53E-04 2,13E-04 1,65384725 1,31993054 0,18685821 LOC100048331 dnaJ homolog subfamily A member 4 2,73E-04 1,42E-04 1,916936283 1,297484975 0,19446437 Gm5678 carbonyl reductase [NADPH] 1 5,18E-04 3,33E-04 1,556716275 1,293699136 0,19576936 CUB and zona pellucida-like domain-containing protein 1 CUZD1 precursor 7,29E-04 5,19E-04 1,40589577 1,289833963 0,19710831 Dusp21 dual specificity phosphatase 21 9,74E-04 5,50E-04 1,772319609 1,287421401 0,19794745 BC089491 selenoprotein V 2,58E-04 1,32E-04 1,949363033 1,287421401 0,19794745 LOC100044248 uncharacterized protein C12orf60 homolog 3,38E-04 2,03E-04 1,662277392 1,283726894 0,19923753 AMDHD2 putative N-acetylglucosamine-6-phosphate deacetylase 3,05E-04 1,70E-04 1,793829191 1,283726894 0,19923753 ACTL9 actin-like protein 9 3,64E-04 2,03E-04 1,792994786 1,283726894 0,19923753 EFCAB3 EF-hand calcium-binding domain-containing protein 3 8,64E-05 3,22E-05 2,679213287 1,264385854 0,20609157 Gm5428 PREDICTED: 60S ribosomal protein L6-like 1,18E-04 3,80E-05 3,103449723 1,264385854 0,20609157 TTC29 tetratricopeptide repeat protein 29 8,25E-05 3,03E-05 2,721496518 1,264385854 0,20609157 CIB1 calcium and integrin-binding protein 1 1,82E-04 6,90E-05 2,64477977 1,264385854 0,20609157 Trap1 heat shock protein 75 kDa, mitochondrial precursor 2,34E-04 1,54E-04 1,520862166 1,243966244 0,21351199

SPACA3 sperm acrosome membrane-associated protein 3 precursor 0,001056748 6,80E-04 1,554550543 1,205921503 0,22784776 4922502D21Rik C-type lectin-like receptor 2m 5,47E-04 3,27E-04 1,673206241 1,204254252 0,22849132 Lcn5 epididymal-specific lipocalin-5 isoform a precursor 9,67E-04 6,25E-04 1,545753876 1,197419843 0,23114295 HIST1H2BA histone H2B type 1-A 3,84E-04 1,90E-04 2,018811794 1,195170039 0,2320206 CCDC105 coiled-coil domain-containing protein 105 0,001754358 0,001457893 1,203351031 1,178970814 0,2384098 NADH-ubiquinone oxidoreductase 75 kDa subunit, NDUFS1 mitochondrial precursor 0,002458881 0,002090475 1,176230509 1,168148515 0,24274686 NADH dehydrogenase [ubiquinone] flavoprotein 2, Ndufv2 mitochondrial isoform 1 precursor 0,002342213 0,001897147 1,234597242 1,16614641 0,24355526 SCAMP1 secretory carrier-associated membrane protein 1 0,003365563 0,002679324 1,256124298 1,15841391 0,24669517 ribC1 RIB43A-like with coiled-coils protein 1 4,22E-04 2,78E-04 1,518130545 1,153702951 0,24862197 KLHL10 kelch-like protein 10 2,83E-04 2,03E-04 1,39565856 1,153702951 0,24862197 DNAJC11 dnaJ homolog subfamily C member 11 4,31E-04 3,09E-04 1,395257496 1,135719571 0,25607395 isochorismatase domain-containing protein 2A, mitochondrial Isoc2a precursor 0,001910477 0,001321028 1,446205366 1,135719571 0,25607395 THEG testicular haploid expressed gene protein isoform 1 0,001899424 0,001544145 1,23008125 1,134646336 0,25652353 Dnahc10 dynein heavy chain 10, axonemal 6,92E-05 5,44E-05 1,272885648 1,113538443 0,26547725 PHB2 prohibitin-2 0,002803321 0,002392801 1,171564906 1,11185287 0,26620142 uncharacterized coiled-coil domain-containing protein 4921530D09Rik KIAA1984 6,75E-04 5,40E-04 1,249703662 1,111621748 0,26630082 Adam4 a disintegrin and metallopeptidase domain 4 precursor 2,61E-04 1,74E-04 1,498347001 1,108020388 0,26785301 p4hb protein disulfide-isomerase precursor 1,45E-04 8,18E-05 1,767853607 1,103482599 0,26981764 Arf6 ADP-ribosylation factor 6 5,13E-04 3,17E-04 1,618800487 1,103482599 0,26981764 zan zonadhesin precursor 0,001237238 0,00110264 1,122068451 1,097477579 0,27243268 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia NAD kinase domain-containing protein 1, mitochondrial 1110020G09Rik isoform 1 9,79E-05 4,56E-05 2,14555578 1,096941157 0,27266712 nagA alpha-N-acetylgalactosaminidase precursor 1,16E-04 5,32E-05 2,171773092 1,096941157 0,27266712 PSMD4 26S proteasome non-ATPase regulatory subunit 4 1,98E-04 9,07E-05 2,181820351 1,096941157 0,27266712 PREDICTED: LOW QUALITY PROTEIN: dynein heavy dnhc3 chain 3, axonemal 1,63E-04 1,34E-04 1,215804266 1,093935858 0,27398311 glud1 glutamate dehydrogenase 1, mitochondrial precursor 3,65E-04 2,70E-04 1,351142079 1,087342387 0,27688552 Smpd5 sphingomyelin phosphodiesterase 5 2,20E-04 1,27E-04 1,727510128 1,084915749 0,27795897 PPA2 inorganic pyrophosphatase 2, mitochondrial precursor 3,20E-04 2,04E-04 1,569532958 1,070146427 0,2845534 Ccdc116 coiled-coil domain-containing protein 116 3,58E-04 2,20E-04 1,628733742 1,070146427 0,2845534 SLC25A20 mitochondrial carnitine/acylcarnitine carrier protein 9,51E-04 7,23E-04 1,316757231 1,065205199 0,28678308 hspb9 heat shock protein beta-9 0,001599544 0,001212609 1,31909391 1,062800259 0,28787254 ccdc65 coiled-coil domain-containing protein 65 2,70E-04 1,89E-04 1,432483634 1,060959891 0,28870813 #N/A insulin-degrading enzyme 1,59E-04 1,08E-04 1,480929009 1,060959891 0,28870813 nagK N-acetyl-D-glucosamine kinase isoform 1 9,80E-04 7,32E-04 1,338223677 1,043217388 0,29684762 1700007I08Rik actin-like protein 10 3,60E-04 2,18E-04 1,64926343 1,02923507 0,30336923 morn5 MORN repeat-containing protein 5 9,39E-04 6,92E-04 1,356421898 1,022477612 0,3065549 LOC100047577 cytochrome b5 type B precursor 0,002030367 0,001506001 1,348184244 1,021878992 0,30683818 MNS1 meiosis-specific nuclear structural protein 1 5,08E-04 3,88E-04 1,309258605 1,012696202 0,31120529 NME7 nucleoside diphosphate kinase 7 isoform 1 5,71E-04 4,14E-04 1,380103214 1,001692488 0,31649214 TEX13A testis-expressed sequence 13A protein 1,06E-04 4,72E-05 2,251287685 0,993457911 0,32048685 cyb5r3 NADH-cytochrome b5 reductase 3 2,55E-04 1,53E-04 1,664554644 0,993055402 0,32068296 - PREDICTED: WD repeat-containing protein 66 2,25E-04 1,63E-04 1,381911728 0,991239197 0,3215688 Izumo1 izumo sperm-egg fusion protein 1 precursor 0,004170218 0,0036192 1,152248614 0,988308407 0,32300163 Dnahc17 dynein heavy chain 17, axonemal 4,04E-04 3,77E-04 1,071035988 0,98344927 0,32538637 Rab1 ras-related protein Rab-1A 2,07E-04 1,12E-04 1,845957381 0,97036406 0,33186506 1700052N19Rik UPF0364 protein C6orf211 homolog 1,75E-04 9,93E-05 1,765106582 0,97036406 0,33186506 Tmem126a transmembrane protein 126A 4,26E-04 2,64E-04 1,615871536 0,97036406 0,33186506 ARMC3 armadillo repeat-containing protein 3 isoform 1 3,88E-04 3,17E-04 1,223337861 0,968740045 0,33267491 chdh choline dehydrogenase, mitochondrial 4,34E-04 3,16E-04 1,373902916 0,968355279 0,33286697 PGD 6-phosphogluconate dehydrogenase, decarboxylating 2,40E-04 1,45E-04 1,652569162 0,963497503 0,33529792 gstk1 glutathione S-transferase kappa 1 3,92E-04 2,58E-04 1,521392978 0,963497503 0,33529792 lmna prelamin-A/C isoform C 1,80E-04 1,23E-04 1,464131525 0,960148227 0,33698062 Samm50 sorting and assembly machinery component 50 homolog 8,06E-04 6,71E-04 1,202034715 0,946957372 0,3436605 Zmynd12 zinc finger, MYND domain containing 12 5,17E-04 3,82E-04 1,351846649 0,946674697 0,34380457 1700012P22Rik uncharacterized protein C1orf158 homolog 9,30E-04 7,01E-04 1,326977161 0,946674697 0,34380457

CATSPERB cation channel sperm-associated protein subunit beta precursor 2,17E-04 1,63E-04 1,330993801 0,946674697 0,34380457 Ces7 carboxylesterase 5A precursor 6,78E-04 5,30E-04 1,277455323 0,946674697 0,34380457 them4 acyl-coenzyme A thioesterase THEM4 0,001125174 8,67E-04 1,297391317 0,936556702 0,34898663 BC049730 uncharacterized protein LOC232972 precursor 0,001393365 0,001111177 1,253954257 0,936556702 0,34898663 Spam1 hyaluronidase PH-20 precursor 7,96E-04 6,42E-04 1,240593733 0,925753684 0,354574 [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, Pdp1 mitochondrial isoform b 1,73E-04 1,11E-04 1,568220688 0,914699326 0,3603495 IMMP1L mitochondrial inner membrane protease subunit 1 4,82E-04 3,01E-04 1,602939962 0,914699326 0,3603495 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial OXSM precursor 3,61E-04 2,47E-04 1,459531392 0,911904675 0,3618189 Pfn3 profilin-3 0,00113044 8,60E-04 1,31482085 0,911813137 0,36186709 1700071K01Rik prohibitin-like 6,35E-04 4,96E-04 1,279613238 0,911813137 0,36186709 SPCS3 signal peptidase complex subunit 3 3,94E-04 2,16E-04 1,819928623 0,86968006 0,38447527 Ubtd2 ubiquitin domain-containing protein 2 3,11E-04 2,02E-04 1,536285615 0,86968006 0,38447527 PSMD6 26S proteasome non-ATPase regulatory subunit 6 1,27E-04 8,03E-05 1,584391082 0,86968006 0,38447527 DSTN destrin 2,34E-04 1,34E-04 1,737538841 0,86968006 0,38447527 PLBD2 putative phospholipase B-like 2 precursor 4,48E-04 3,39E-04 1,319088785 0,851692729 0,39438466 psmd7 26S proteasome non-ATPase regulatory subunit 7 6,07E-04 4,74E-04 1,281035787 0,851692729 0,39438466 1700061G19Rik uncharacterized protein LOC78625 3,61E-04 2,75E-04 1,315121041 0,84722899 0,3968675 HYDIN hydrocephalus-inducing protein 8,17E-05 6,73E-05 1,213786416 0,841056709 0,40031617 TXNRD1 thioredoxin reductase 1, cytoplasmic isoform 2 8,57E-05 4,42E-05 1,938312053 0,840347729 0,40071345 COPS7A COP9 signalosome complex subunit 7a isoform 2 1,61E-04 9,09E-05 1,771141748 0,840347729 0,40071345 TXNDC3 thioredoxin domain-containing protein 3 isoform 1 0,001087846 9,44E-04 1,152919792 0,826389101 0,4085834 cct5 T-complex protein 1 subunit epsilon 4,54E-04 3,65E-04 1,24573158 0,822375795 0,41086305 ME2 NAD-dependent malic enzyme, mitochondrial precursor 4,31E-04 3,40E-04 1,265596099 0,81720185 0,41381309 Tubb2a-ps2 tubulin beta-2B chain 3,13E-04 2,49E-04 1,255027202 0,817138223 0,41384944 Sept12 septin-12 isoform 1 4,29E-04 3,17E-04 1,354356006 0,802958413 0,42199877 - regulator of G-protein signaling 22 1,13E-04 8,41E-05 1,33935539 0,802958413 0,42199877 ACTL7A actin-like protein 7A 0,00130456 0,001120965 1,163783453 0,79157783 0,42860688 cation channel sperm-associated protein subunit gamma 2 Catsperg2 precursor 1,97E-04 1,54E-04 1,282459509 0,787241951 0,43114024 1700006E09Rik uncharacterized protein C17orf105 homolog 8,97E-04 6,24E-04 1,436447305 0,787241951 0,43114024 Fam162a protein FAM162A 5,87E-04 3,49E-04 1,683554274 0,786661964 0,43147978 1700019O17Rik uncharacterized protein C2orf57 homolog 3,13E-04 2,08E-04 1,504534374 0,786661964 0,43147978 camk4 calcium/calmodulin-dependent protein kinase type IV 2,60E-04 1,82E-04 1,427842507 0,786577863 0,43152902 CCDC113 coiled-coil domain-containing protein 113 2,46E-04 1,58E-04 1,557161645 0,786577863 0,43152902 Tmem11 transmembrane protein 11, mitochondrial isoform 1 6,71E-04 4,67E-04 1,437301427 0,786577863 0,43152902 BPI bactericidal permeability-increasing protein precursor 2,49E-04 1,85E-04 1,346910739 0,786577863 0,43152902

SIRT5 NAD-dependent protein deacylase sirtuin-5, mitochondrial 3,13E-04 2,27E-04 1,378764468 0,786577863 0,43152902 csnk2a2 casein kinase II subunit alpha 0,00233044 0,001957684 1,190406922 0,780130081 0,43531431 Pdia6 PREDICTED: protein disulfide-isomerase A6 4,04E-04 3,02E-04 1,340251073 0,758476347 0,44816587 Glipr1l3 GLI pathogenesis-related 1 like 1 precursor 9,98E-04 7,73E-04 1,291019034 0,753396269 0,45121182 lonp1 lon protease homolog, mitochondrial precursor 1,89E-04 1,47E-04 1,283229489 0,751028774 0,45263534 lrrc48 leucine-rich repeat-containing protein 48 3,74E-04 2,93E-04 1,277120973 0,751028774 0,45263534 arsA arylsulfatase A precursor 7,11E-04 5,60E-04 1,269781319 0,751028774 0,45263534 TMEM30A cell cycle control protein 50A 2,05E-04 1,17E-04 1,752440705 0,727740362 0,46677254 Ggct gamma-glutamylcyclotransferase 2,07E-04 1,21E-04 1,706460957 0,727740362 0,46677254 tmed10 transmembrane emp24 domain-containing protein 10 precursor 2,74E-04 1,71E-04 1,599755729 0,727740362 0,46677254 CYB5R1 NADH-cytochrome b5 reductase 1 1,96E-04 1,23E-04 1,592344918 0,727740362 0,46677254 Ribc2 RIB43A-like with coiled-coils protein 2 4,24E-04 3,29E-04 1,290946437 0,716977305 0,4733881 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia mitochondrial translocator assembly and maintenance protein 1500001M20Rik 41 homolog precursor 3,44E-04 2,34E-04 1,469004467 0,716357815 0,47377044 SCAMP2 secretory carrier-associated membrane protein 2 0,002927815 0,002428249 1,205730634 0,709433552 0,47805547 serine/threonine-protein phosphatase 2B catalytic subunit PPP3CC gamma isoform 5,20E-04 4,25E-04 1,223200497 0,695726234 0,48660029 manf mesencephalic astrocyte-derived neurotrophic factor precursor 1,12E-04 5,66E-05 1,972317975 0,686131088 0,49263045 1700019B03Rik uncharacterized protein LOC76406 5,85E-05 2,99E-05 1,956099678 0,686131088 0,49263045 RNASE13 probable inactive ribonuclease-like protein 13 precursor 2,69E-04 1,37E-04 1,96717649 0,686131088 0,49263045 Svs5 seminal vesicle secretory protein 5 precursor 3,82E-04 1,91E-04 2,000862392 0,686131088 0,49263045 FLAD1 FAD synthase 5,40E-05 2,94E-05 1,834554397 0,686131088 0,49263045 FDX1L adrenodoxin-like protein, mitochondrial precursor 1,50E-04 8,18E-05 1,828370306 0,686131088 0,49263045 BEST1 bestrophin-1 6,66E-05 3,60E-05 1,848110758 0,686131088 0,49263045 tk2 thymidine kinase 2, mitochondrial precursor 1,09E-04 6,38E-05 1,701890073 0,686131088 0,49263045 LRRC34 leucine-rich repeat-containing protein 34 3,04E-04 2,23E-04 1,365298489 0,684641114 0,49357042 xpo1 exportin-1 1,38E-04 1,07E-04 1,295790444 0,684641114 0,49357042 CCDC40 coiled-coil domain-containing protein 40 3,78E-04 3,26E-04 1,15889967 0,682551352 0,49489038 PSMD1 26S proteasome non-ATPase regulatory subunit 1 4,69E-04 4,01E-04 1,170276253 0,682551352 0,49489038 PREDICTED: phosphate carrier protein, mitochondrial-like SLC25A3 isoform 1 9,44E-04 7,82E-04 1,206502717 0,680748821 0,49603044 Lrrc57 leucine-rich repeat-containing protein 57 isoform b 5,90E-04 4,29E-04 1,374284066 0,67929925 0,49694827 hibadh 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor 0,001939088 0,0017461 1,110525097 0,677575533 0,49804086 ODF1 outer dense fiber protein 1 0,001893666 0,001646346 1,150223664 0,669237838 0,50334377 Nme1 nucleoside diphosphate kinase A 5,52E-04 4,17E-04 1,323095212 0,655581074 0,51209371 tomm40 mitochondrial import receptor subunit TOM40 homolog 5,04E-04 3,76E-04 1,338702001 0,640667263 0,52173889 EEF1E1 eukaryotic translation elongation factor 1 epsilon-1 2,95E-04 2,25E-04 1,309382625 0,639635388 0,52240967 Efcab2 EF-hand calcium-binding domain-containing protein 2 4,24E-04 2,93E-04 1,448207201 0,639635388 0,52240967 1700009N14Rik uncharacterized protein LOC75471 2,11E-04 1,49E-04 1,419787697 0,639635388 0,52240967 1700019L03Rik uncharacterized protein C9orf117 homolog 1,07E-04 7,60E-05 1,403951951 0,639635388 0,52240967 PFKM 6-phosphofructokinase, muscle type 4,01E-04 3,43E-04 1,167081389 0,639098369 0,52275894 Spa17 sperm surface protein Sp17 0,001519277 0,001169626 1,298942408 0,639098369 0,52275894 Sept4 septin-4 7,23E-04 5,81E-04 1,243344981 0,61978752 0,53539769 LOC630729 glutathione reductase, mitochondrial precursor 2,33E-04 1,72E-04 1,354862807 0,602173589 0,54705859

Tmco2 transmembrane and coiled-coil domain-containing protein 2 0,001048317 8,70E-04 1,204929608 0,600365914 0,5482624

UCHL5 ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 1 4,20E-04 3,42E-04 1,228170096 0,600365914 0,5482624 4930579J09Rik protein phosphatase 1 regulatory subunit 32 5,99E-04 5,18E-04 1,156750689 0,571937905 0,56736404 4930412F15Rik protein FAM221B 1,56E-04 1,17E-04 1,32637584 0,567736129 0,57021416 FKBP1A peptidyl-prolyl cis-trans isomerase FKBP1A 5,37E-04 4,14E-04 1,297242229 0,567736129 0,57021416 RSPH9 radial spoke head protein 9 homolog 0,002326372 0,00200432 1,160678759 0,565558498 0,57169395 prmt5 protein arginine N-methyltransferase 5 2,59E-04 2,07E-04 1,252767387 0,563191874 0,57330424 Dynlrb2 dynein light chain roadblock-type 2 0,001193492 8,66E-04 1,377763863 0,563191874 0,57330424 BANF2 barrier-to-autointegration factor-like protein 2,38E-04 1,52E-04 1,568980215 0,556232478 0,57805194 atp6v1b2 V-type proton ATPase subunit B, brain isoform 6,87E-05 4,60E-05 1,49182376 0,556232478 0,57805194 very long-chain specific acyl-CoA dehydrogenase, ACADVL mitochondrial precursor 4,78E-05 3,15E-05 1,518261832 0,556232478 0,57805194 CCDC33 coiled-coil domain-containing protein 33 isoform 1 4,21E-05 2,74E-05 1,536541526 0,556232478 0,57805194 ARF4 ADP-ribosylation factor 4 2,01E-04 1,41E-04 1,432227572 0,556232478 0,57805194 USP5 ubiquitin carboxyl-terminal hydrolase 5 4,53E-05 3,10E-05 1,464207323 0,556232478 0,57805194 ENO4 enolase-like protein ENO4 8,15E-05 5,38E-05 1,514766447 0,556232478 0,57805194 DNAJB8 dnaJ homolog subfamily B member 8 1,69E-04 1,07E-04 1,583865964 0,556232478 0,57805194 ERI3 ERI1 exoribonuclease 3 1,74E-04 1,18E-04 1,474484464 0,556232478 0,57805194 1700094D03Rik uncharacterized protein C1orf189 homolog 2,78E-04 1,76E-04 1,584621811 0,556232478 0,57805194 gtl3 UPF0468 protein C16orf80 homolog 0,001469589 0,001235519 1,189450726 0,55602672 0,57819259 ahcY adenosylhomocysteinase 2,13E-04 1,58E-04 1,349366106 0,554613307 0,57915919 phospholipid hydroperoxide glutathione peroxidase, nuclear Gpx4 isoform 1 0,001711689 0,00149395 1,145746972 0,538284394 0,59038072 NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, NDUFS7 mitochondrial precursor 0,002184117 0,002011823 1,085640903 0,537604572 0,59085007 Fam71a uncharacterized protein LOC619288 0,001124802 9,54E-04 1,17910369 0,536886887 0,59134575 Rsph3b radial spoke head protein 3 homolog B 8,81E-04 7,99E-04 1,102861025 0,536858272 0,59136552 KRT2 keratin, type II cytoskeletal 2 epidermal 3,30E-04 3,00E-04 1,099992385 0,528708891 0,59700741 EFCAB5 EF-hand calcium-binding domain-containing protein 5 1,34E-04 1,15E-04 1,166511254 0,52176354 0,60183498 CCDC42 coiled-coil domain-containing protein 42A 5,10E-04 4,34E-04 1,174916057 0,52176354 0,60183498 coiled-coil-helix-coiled-coil-helix domain-containing protein 6, CHCHD6 mitochondrial isoform 1 0,00148473 0,001205515 1,231614216 0,512402512 0,60836933 CAR1 carbonic anhydrase 1 5,34E-04 4,08E-04 1,309620419 0,511790914 0,60879735 Actrt2 actin-related protein T2 2,84E-04 2,23E-04 1,272278645 0,511790914 0,60879735 Cfl2 cofilin-2 4,42E-04 3,47E-04 1,273405532 0,511790914 0,60879735 Rsph1 radial spoke head 1 homolog 0,001243148 0,001102787 1,127278543 0,508362585 0,61119909 DDI1 protein DDI1 homolog 1 2,59E-04 1,99E-04 1,298945522 0,507245039 0,6119829 ACAA2 3-ketoacyl-CoA thiolase, mitochondrial 1,97E-04 1,44E-04 1,375957999 0,507245039 0,6119829 TCP1 T-complex protein 1 subunit alpha 0,001056226 9,71E-04 1,087890176 0,506647358 0,61240227 REEP6 receptor expression-enhancing protein 6 isoform 1 0,001100395 0,001041048 1,057007385 0,496563012 0,61949724 Pacrg parkin coregulated gene protein homolog 5,88E-04 4,93E-04 1,193893985 0,496563012 0,61949724 PREDICTED: ES1 protein homolog, mitochondrial-like LOC100046684 isoform 1 6,91E-04 5,58E-04 1,238268037 0,495274396 0,62040644 Tmem190 transmembrane protein 190 precursor 0,002222989 0,001909063 1,164439887 0,495274396 0,62040644 Acr acrosin isoform 3 0,003268046 0,003184981 1,026080439 0,494595301 0,62088582

COX7A2 cytochrome c oxidase subunit 7A2, mitochondrial precursor 0,00145053 0,001138431 1,274148425 0,472875589 0,63630194 Acrv1 acrosomal protein SP-10 precursor 0,006702331 0,005655238 1,185154379 0,471319577 0,63741254 CCT8L1 chaperonin containing TCP1, subunit 8 (theta)-like 1 4,19E-04 3,54E-04 1,180867876 0,47007734 0,63829976 1700023E05Rik testicular haploid expressed gene protein-like 2,94E-04 2,58E-04 1,136868347 0,466463514 0,64088376 NUDCD2 nudC domain-containing protein 2 2,80E-04 2,01E-04 1,389005729 0,463543393 0,64297492 PLP1 myelin proteolipid protein 2,56E-04 1,70E-04 1,503397419 0,463543393 0,64297492 EFCAB10 EF-hand calcium-binding domain-containing protein 10 3,73E-04 2,57E-04 1,448958768 0,463543393 0,64297492 Nsf vesicle-fusing ATPase 4,92E-05 3,55E-05 1,384366276 0,463543393 0,64297492 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia COPS4 COP9 signalosome complex subunit 4 9,97E-05 7,47E-05 1,335207573 0,463543393 0,64297492 NME2 nucleoside diphosphate kinase B 5,06E-04 4,23E-04 1,197004477 0,45516428 0,64899106 ACYP1 acylphosphatase-1 5,42E-04 4,66E-04 1,16299709 0,45516428 0,64899106 Cct7 T-complex protein 1 subunit eta 7,26E-04 6,52E-04 1,113455849 0,451508701 0,65162296 ENKUR enkurin 0,001354008 0,001294439 1,046019267 0,449351371 0,65317821 Gykl1 glycerol kinase-like 1 0,002044224 0,00199479 1,024781489 0,44293087 0,65781574 CSNK1G2 casein kinase I isoform gamma-2 isoform 1 4,30E-04 3,92E-04 1,09751319 0,438168986 0,66126379 disintegrin and metalloproteinase domain-containing protein 5 Adam5 isoform 1 precursor 2,01E-04 1,70E-04 1,183412313 0,438168986 0,66126379 serine/threonine-protein phosphatase PP1-gamma catalytic LOC627816 subunit 3,72E-04 3,22E-04 1,154684182 0,437230651 0,66194408 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex Ndufa2 subunit 2 8,07E-04 6,89E-04 1,171626434 0,437230651 0,66194408 cul3 cullin-3 4,26E-04 3,65E-04 1,166854248 0,434475305 0,66394332 Gm15785 dnaJ homolog subfamily B member 6 isoform a 0,001012886 8,92E-04 1,135446432 0,432936143 0,66506116 CCDC151 coiled-coil domain-containing protein 151 isoform 2 4,94E-04 4,25E-04 1,16422116 0,432645306 0,66527247 Atp1b3 sodium/potassium-transporting ATPase subunit beta-3 0,001401258 0,001287394 1,088445569 0,432645306 0,66527247 Uchl3 ubiquitin carboxyl-terminal hydrolase isozyme L3 9,64E-04 8,63E-04 1,116155759 0,42309719 0,67222435 LOC100044101 thioredoxin reductase 2, mitochondrial precursor 1,96E-04 1,67E-04 1,175980202 0,409508401 0,6821666 TCTE1 T-complex-associated testis-expressed protein 1 2,40E-04 1,71E-04 1,399960872 0,409508401 0,6821666 SH3GL3 endophilin-A3 isoform 1 2,32E-04 1,92E-04 1,205726537 0,409508401 0,6821666 Gstt4 glutathione S-transferase theta-4 3,28E-04 2,82E-04 1,163095326 0,409508401 0,6821666 BANF1 barrier-to-autointegration factor 8,07E-04 6,22E-04 1,29713933 0,409508401 0,6821666 rpiA ribose-5-phosphate isomerase 2,53E-04 2,20E-04 1,14915706 0,409508401 0,6821666 CES3 carboxylesterase 1D precursor 1,17E-04 9,21E-05 1,266739403 0,39214806 0,69494882 Efcab9 EF-hand calcium-binding domain-containing protein 9 2,95E-04 2,33E-04 1,268233456 0,39214806 0,69494882 C8orfk29 PREDICTED: putative transmembrane protein C8orfK29 1,80E-04 1,54E-04 1,168542315 0,39214806 0,69494882 CKLF-like MARVEL transmembrane domain-containing Cmtm2b protein 2B 3,49E-04 2,68E-04 1,305025011 0,39214806 0,69494882 PSMC2 26S protease regulatory subunit 7 2,64E-04 2,37E-04 1,111414261 0,3862163 0,69933648 PSMB5 proteasome subunit beta type-5 0,001341194 0,00116698 1,149285457 0,371607362 0,71018521 4931432M23Rik inactive serine protease 54 precursor 3,66E-04 3,17E-04 1,155363182 0,368901473 0,71220117 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex Ndufa8 subunit 8 0,001611923 0,001363709 1,182013723 0,340287644 0,73363992 KRT10 keratin, type I cytoskeletal 10 6,04E-04 5,47E-04 1,102914277 0,339425234 0,73428941 CASC1 cancer susceptibility candidate protein 1 2,73E-04 2,45E-04 1,113934272 0,339425234 0,73428941 COX6B1 cytochrome c oxidase subunit 6B1 3,39E-04 2,74E-04 1,236051829 0,334350885 0,7381148 NDUFAB1 acyl carrier protein, mitochondrial precursor 4,13E-04 3,44E-04 1,203112988 0,334350885 0,7381148 HIST2H3B histone H2A type 2-A 4,78E-04 3,86E-04 1,238239606 0,334350885 0,7381148 - uncharacterized protein LOC69439 1,24E-04 1,12E-04 1,103692542 0,332354661 0,73962147 Acat3 acetyl-Coenzyme A acetyltransferase 3 6,62E-05 4,90E-05 1,350865367 0,327766748 0,74308803 DPY19L1 probable C-mannosyltransferase DPY19L1 4,71E-05 3,55E-05 1,325433464 0,327766748 0,74308803 mitochondrial import inner membrane translocase subunit 1700034H14Rik Tim21 6,50E-05 4,49E-05 1,44566188 0,327766748 0,74308803 FAM24A protein FAM24A precursor 2,41E-04 1,70E-04 1,413728747 0,327766748 0,74308803 1700040L02Rik uncharacterized protein C10orf107 homolog 8,74E-05 6,29E-05 1,390399348 0,327766748 0,74308803 - PREDICTED: uncharacterized protein LOC380654 9,08E-06 6,66E-06 1,363668151 0,327766748 0,74308803 arl1 ADP-ribosylation factor-like protein 1 1,10E-04 8,36E-05 1,321517073 0,327766748 0,74308803 Cnpy2 protein canopy homolog 2 precursor 9,53E-05 6,68E-05 1,42563412 0,327766748 0,74308803 LOC100046536 PREDICTED: glutathione S-transferase P 2-like 1,46E-04 1,07E-04 1,370364028 0,327766748 0,74308803 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA10 subunit 10, mitochondrial precursor 0,001794734 0,001667841 1,076082176 0,323847622 0,7460534 Gm5506 alpha-enolase 0,004177317 0,004099178 1,019061945 0,320656363 0,74847082 Gm4763 uncharacterized protein LOC210155 0,001270718 0,001107806 1,147058066 0,317660957 0,75074213 cct4 T-complex protein 1 subunit delta 8,64E-04 7,93E-04 1,090239282 0,312695067 0,75451234 Dnahc2 dynein heavy chain 2, axonemal 7,41E-05 6,92E-05 1,069741175 0,31143019 0,75547361 SSSCA1 Sjoegren syndrome/scleroderma autoantigen 1 homolog 6,94E-04 6,13E-04 1,130988222 0,2991358 0,76483643 PSMB4 proteasome subunit beta type-4 precursor 9,17E-04 8,27E-04 1,108062353 0,296853659 0,76657824 Gm3982 mitochondrial pyruvate carrier 2 0,004985642 0,004475488 1,113988318 0,289437313 0,77224674 Wfdc15b WAP four-disulfide core domain protein 15B precursor 6,85E-04 5,36E-04 1,277834227 0,285902527 0,77495278 Gm9093 60S acidic ribosomal protein P0 2,19E-04 1,91E-04 1,149572895 0,285902527 0,77495278 SEC11A signal peptidase complex catalytic subunit SEC11A 3,09E-04 2,46E-04 1,254171487 0,285902527 0,77495278 LOC100048880 AFG3-like protein 2 2,34E-04 2,09E-04 1,116180992 0,276920777 0,78184094 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit NDUFB10 10 8,87E-04 7,95E-04 1,115985549 0,276920777 0,78184094 PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur Ndufs5 protein 5-like 7,70E-04 6,88E-04 1,120039323 0,268689092 0,78816895 Ccdc114 coiled-coil domain-containing protein 114 1,86E-04 1,63E-04 1,142511687 0,268689092 0,78816895 PHOSPHO1 phosphoethanolamine/phosphocholine phosphatase 0,001129927 0,001029244 1,097822485 0,265277692 0,79079555 TTC18 tetratricopeptide repeat domain 18 isoform 3 2,65E-04 2,43E-04 1,089820139 0,2577897 0,79656921 macrod1 O-acetyl-ADP-ribose deacetylase MACROD1 2,08E-04 1,73E-04 1,202167231 0,244232722 0,80705059 APEH acylamino-acid-releasing enzyme 1,29E-04 1,14E-04 1,134577622 0,244232722 0,80705059 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA11 subunit 11 6,54E-04 6,15E-04 1,063368118 0,244232722 0,80705059 1700065I17Rik uncharacterized protein C5orf48 homolog 5,59E-04 5,36E-04 1,041851775 0,244232722 0,80705059 SHMT1 serine hydroxymethyltransferase, cytosolic 1,51E-04 1,34E-04 1,129609958 0,244232722 0,80705059 Gm12231 ATP synthase subunit b, mitochondrial precursor 0,001597778 0,001575675 1,014027709 0,243532483 0,80759292 rpl12 60S ribosomal protein L12 5,99E-04 4,97E-04 1,206329031 0,2405836 0,80987786 high affinity cAMP-specific and IBMX-insensitive 3,5-cyclic PDE8A phosphodiesterase 8A 1,67E-04 1,53E-04 1,09660894 0,2405836 0,80987786 Cct3 T-complex protein 1 subunit gamma 0,001423076 0,001324683 1,074276852 0,239409434 0,81078812 Psma3 proteasome subunit alpha type-3 7,26E-04 6,37E-04 1,140163982 0,239396262 0,81079833

Gm5436 PREDICTED: ATP synthase subunit O, mitochondrial-like 0,003181558 0,002824391 1,126458195 0,237168601 0,81252599 pbp2 phosphatidylethanolamine-binding protein 2 2,03E-04 1,65E-04 1,226724768 0,236413771 0,81311161 IQCF1 IQ domain-containing protein F1 isoform 1 2,14E-04 1,49E-04 1,442370846 0,236413771 0,81311161 1700021F07Rik uncharacterized protein C20orf85 homolog 2,33E-04 1,62E-04 1,434453099 0,236413771 0,81311161 Spag8 sperm-associated antigen 8 1,08E-04 8,42E-05 1,279902318 0,236413771 0,81311161 CYB5 cytochrome b5 2,43E-04 2,01E-04 1,206329524 0,236413771 0,81311161 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia

2310028O11Rik mitochondrial inner membrane organizing system protein 1 3,77E-04 3,18E-04 1,186361651 0,236413771 0,81311161 Gm166 coiled-coil domain-containing protein C16orf93 homolog 1,06E-04 9,04E-05 1,178100683 0,236413771 0,81311161 Psma4 proteasome subunit alpha type-4 6,73E-04 6,22E-04 1,082711823 0,226388857 0,82089899 Car4 carbonic anhydrase 4 precursor 0,001791858 0,001673315 1,070843355 0,217128085 0,82810853 NME5 nucleoside diphosphate kinase homolog 5 7,17E-04 6,78E-04 1,057076653 0,214479073 0,83017348 SATL1 spermidine/spermine N(1)-acetyltransferase-like protein 1 1,85E-04 1,55E-04 1,196281011 0,207679548 0,83547919 Styxl1 serine/threonine/tyrosine-interacting-like protein 1 2,41E-04 2,22E-04 1,085143759 0,207679548 0,83547919 1110032A03Rik UPF0686 protein C11orf1 homolog 6,59E-04 5,89E-04 1,11888808 0,207679548 0,83547919 Fam71e2 uncharacterized protein LOC243822 3,24E-04 2,96E-04 1,092823341 0,206116024 0,83670028 dihydrolipoyllysine-residue succinyltransferase component of 2- DLST oxoglutarate dehydrogenase complex, mitochondrial 0,001393699 0,0012896 1,080721721 0,200499352 0,84109006 UQCRQ cytochrome b-c1 complex subunit 8 0,001322319 0,001226143 1,078437583 0,190102585 0,84922874 cation channel sperm-associated protein subunit delta TMEM146 precursor 2,89E-04 2,79E-04 1,038080301 0,190102585 0,84922874 ACSL1 long-chain-fatty-acid--CoA ligase 1 0,002442966 0,002365198 1,032879808 0,188363182 0,85059196 IMMP2L mitochondrial inner membrane protease subunit 2 4,22E-04 3,76E-04 1,121712967 0,175112108 0,86099154 Adgb androglobin 6,04E-05 5,44E-05 1,109194541 0,175112108 0,86099154 pyruvate dehydrogenase phosphatase regulatory subunit, 4930402E16Rik mitochondrial precursor 1,14E-04 1,11E-04 1,027674823 0,175112108 0,86099154 LOC674469 ATP synthase subunit d, mitochondrial 0,003109368 0,002979167 1,043703866 0,17283676 0,86277974 TCEB1 transcription elongation factor B polypeptide 1 3,01E-04 2,75E-04 1,092093667 0,172691093 0,86289425 Gm7684 E3 ubiquitin-protein ligase MARCH5 isoform 1 1,24E-04 1,11E-04 1,115503963 0,172691093 0,86289425 eif5a eukaryotic translation initiation factor 5A-1 2,78E-04 2,50E-04 1,11302853 0,172691093 0,86289425 Rexo2 oligoribonuclease, mitochondrial precursor 1,31E-04 1,15E-04 1,138988646 0,172691093 0,86289425 - uncharacterized protein LOC627607 7,29E-05 6,71E-05 1,085978653 0,172691093 0,86289425 low molecular weight phosphotyrosine protein phosphatase acp1 isoform 2 1,87E-04 1,70E-04 1,101807265 0,172691093 0,86289425 1700024G13Rik UPF0728 protein C10orf53 homolog 3,47E-04 3,11E-04 1,114829249 0,172691093 0,86289425 NUDT2 bis(5-nucleosyl)-tetraphosphatase [asymmetrical] 1,99E-04 1,84E-04 1,082239638 0,172691093 0,86289425 iron-sulfur cluster assembly 1 homolog, mitochondrial iscA1 precursor 2,06E-04 1,69E-04 1,220103277 0,172691093 0,86289425 ARHGDIA rho GDP-dissociation inhibitor 1 1,93E-04 1,49E-04 1,295952716 0,172691093 0,86289425 PREDICTED: transmembrane emp24 domain-containing Tmed9 protein 2-like 2,02E-04 2,02E-04 1,000718506 0,172691093 0,86289425 MORN2 MORN repeat-containing protein 2 4,02E-04 3,44E-04 1,170900248 0,172691093 0,86289425 Thnsl1 threonine synthase-like 1 isoform a 5,32E-04 4,98E-04 1,069633493 0,157827589 0,87459266 Fam71b protein FAM71B 0,002278478 0,002246792 1,014103083 0,157795217 0,87461817 ak7 adenylate kinase 7 7,94E-04 7,68E-04 1,033886342 0,13132628 0,8955172 LOC100048601 probable threonine protease PRSS50 precursor 5,91E-04 5,62E-04 1,050941088 0,125309198 0,90027877 PLIN3 perilipin-3 1,17E-04 1,09E-04 1,071124752 0,123816369 0,90146067 napA alpha-soluble NSF attachment protein 1,20E-04 1,09E-04 1,107315464 0,123816369 0,90146067 PPP6C serine/threonine-protein phosphatase 6 catalytic subunit 1,24E-04 1,22E-04 1,013383397 0,123816369 0,90146067 UBR4 E3 ubiquitin-protein ligase UBR4 1,16E-05 1,05E-05 1,104156646 0,123816369 0,90146067 GPX6 glutathione peroxidase 6 precursor 2,77E-04 2,43E-04 1,1379589 0,123816369 0,90146067 Dnahc6 axonemal dynein heavy chain 1,47E-04 1,44E-04 1,023384728 0,121671894 0,90315887 Lypd4 ly6/PLAUR domain-containing protein 4 precursor 0,002375705 0,002228462 1,066073993 0,121439554 0,90334289 Lrrc51 leucine-rich repeat-containing protein 51 isoform 1 5,85E-04 5,13E-04 1,142004166 0,118943883 0,90531981 psmd3 26S proteasome non-ATPase regulatory subunit 3 2,67E-04 2,63E-04 1,013007096 0,105976691 0,91560085 mitochondrial import inner membrane translocase subunit timm50 TIM50 precursor 5,59E-04 5,00E-04 1,117064886 0,105976691 0,91560085 amyotrophic lateral sclerosis 2 (juvenile) chromosome region, Als2cr11 candidate 11 3,28E-04 3,10E-04 1,05611229 0,105976691 0,91560085 1700001C02Rik UPF0573 protein C2orf70 homolog 0,00141031 0,001307938 1,078270197 0,099357239 0,92085463

SSBP1 single-stranded DNA-binding protein, mitochondrial isoform 1 6,04E-04 5,46E-04 1,106920972 0,094315055 0,92485889 atp5s ATP synthase subunit s, mitochondrial precursor 7,10E-04 6,53E-04 1,087511637 0,087579155 0,93021117 PSMC5 26S protease regulatory subunit 8 3,25E-04 2,95E-04 1,099706614 0,087579155 0,93021117 isyna1 inositol-3-phosphate synthase 1 1,43E-04 1,31E-04 1,094005598 0,084100736 0,93297634 delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial ALDH4A1 precursor 9,99E-05 9,16E-05 1,091274763 0,084100736 0,93297634 SLC22A14 solute carrier family 22 member 14 1,37E-04 1,39E-04 1,010113389 0,084100736 0,93297634 hprT hypoxanthine-guanine phosphoribosyltransferase 1,83E-04 1,76E-04 1,042044495 0,084100736 0,93297634 PPM1B protein phosphatase 1B isoform 1 1,13E-04 9,86E-05 1,145859063 0,084100736 0,93297634 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA1 subunit 1 5,95E-04 5,57E-04 1,069425442 0,084100736 0,93297634 G-protein coupled receptor family C group 5 member B Gprc5b isoform 2 precursor 3,72E-04 3,39E-04 1,094808905 0,084100736 0,93297634 Ccdc19 nasopharyngeal epithelium specific protein 1 6,64E-04 6,29E-04 1,055704713 0,077761158 0,93801804 Prss46 serine protease 46 0,00210585 0,002252203 1,069498153 0,077761158 0,93801804 ASRGL1 isoaspartyl peptidase/L-asparaginase 0,001535239 0,001455404 1,054854167 0,076528933 0,9389983 MORN3 MORN repeat-containing protein 3 4,09E-04 3,83E-04 1,06742853 0,07150289 0,94299752 ACTB actin, cytoplasmic 1 0,001476232 0,001454542 1,014911893 0,071149329 0,94327891

HIBCH 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial precursor 3,99E-04 3,93E-04 1,016459318 0,07002394 0,94417461 Gm128 protein MENT precursor 0,009328123 0,009205995 1,013266065 0,068672624 0,94525021 Gk2 glycerol kinase 2 0,001827615 0,00170677 1,070803338 0,065795713 0,94754047

Fahd2a fumarylacetoacetate hydrolase domain-containing protein 2A 0,002582043 0,00253361 1,019116467 0,062951743 0,94980493 Actc1 actin, alpha cardiac muscle 1 1,21E-04 1,19E-04 1,015011143 0,050556508 0,95967892 Akap14 A-kinase anchor protein 14 1,06E-04 9,92E-05 1,066313288 0,050556508 0,95967892 LOC100045999 PREDICTED: GTP-binding nuclear protein Ran-like 2,48E-04 2,42E-04 1,023780601 0,050556508 0,95967892 4931409K22Rik IQ and AAA domain-containing protein 1-like 1,24E-04 1,18E-04 1,053266676 0,050240728 0,95993056 serine/threonine-protein phosphatase 2A catalytic subunit beta PPP2CB isoform 3,92E-04 3,75E-04 1,046966009 0,050240728 0,95993056 Tssk1 testis-specific serine/threonine-protein kinase 1 7,30E-04 7,00E-04 1,043188474 0,047945172 0,96175994 HSPA9 stress-70 protein, mitochondrial 0,001881744 0,001867697 1,007521286 0,047364338 0,96222285 CCDC11 coiled-coil domain-containing protein 11 3,08E-04 2,97E-04 1,035972198 0,037132058 0,97037971 vapa vesicle-associated membrane protein-associated protein A 6,27E-04 5,76E-04 1,088293398 0,030315704 0,97581527 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia Slc22a21 solute carrier family 22 member 21 4,58E-04 4,77E-04 1,042380578 0,030315704 0,97581527 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit Ndufb8 8, mitochondrial precursor 0,00126805 0,001206639 1,050893567 0,029419823 0,97652976 Gm13570 brain protein 44-like protein 2 0,002277964 0,001994798 1,141952316 0,029419823 0,97652976 psmA2 proteasome subunit alpha type-2 0,001033864 0,001051914 1,01745882 0,021665993 0,98271439 Cyct cytochrome c, testis-specific 0,001382033 0,001337479 1,033311668 0,021665993 0,98271439 LTA4H leukotriene A-4 hydrolase 1,05E-04 1,07E-04 1,018240503 0,021434677 0,98289891 SSNA1 Sjoegren syndrome nuclear autoantigen 1 homolog 4,35E-04 4,31E-04 1,00973147 0,021434677 0,98289891 4921536K21Rik uncharacterized protein C5orf52 homolog 3,53E-04 3,19E-04 1,105291064 0,021434677 0,98289891 4921517D21Rik uncharacterized protein LOC67722 7,56E-05 7,54E-05 1,002194388 0,021434677 0,98289891 small glutamine-rich tetratricopeptide repeat-containing protein Sgta alpha 2,39E-04 2,35E-04 1,01453774 0,021434677 0,98289891 GSTT3 glutathione S-transferase, theta 3 2,91E-04 2,97E-04 1,021848758 0,021434677 0,98289891 2410015M20Rik protein QIL1 precursor 7,14E-04 6,05E-04 1,179808 0,021434677 0,98289891 SPINT4 kunitz-type protease inhibitor 4 precursor 4,01E-04 3,71E-04 1,080993564 0,021434677 0,98289891 Sod2 superoxide dismutase [Mn], mitochondrial precursor 0,001968067 0,001912007 1,029319927 0,012542005 0,98999319 N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase isoform 1 aga precursor 4,81E-04 4,65E-04 1,034735661 0,011555378 0,99078035 Gm10108 cytochrome c, somatic 9,02E-04 8,99E-04 1,00382872 0,011555378 0,99078035 Capzb F-actin-capping protein subunit beta isoform c 0,001279883 0,001223566 1,046026661 0,007179318 0,99427178 4932412H11Rik leucine-rich repeat and death domain-containing protein 1 3,00E-04 2,82E-04 1,062237012 0,00678024 0,99459019 Gm884 uncharacterized protein LOC380730 0,001421706 0,001427589 1,004138045 0,0058535 0,99532961 LETM1 and EF-hand domain-containing protein 1, LETM1 mitochondrial precursor 4,64E-04 4,59E-04 1,009891 0,001262567 0,99899262 4930578I06Rik uncharacterized protein C8orf74 homolog 2,86E-04 2,66E-04 1,076695468 -0,004370765 0,99651265 CATSPER4 cation channel sperm-associated protein 4 isoform 2 2,50E-04 2,62E-04 1,048094839 -0,004370765 0,99651265 FAM78A protein FAM78A 3,50E-04 3,63E-04 1,037552298 -0,004370765 0,99651265 Arrdc5 arrestin domain-containing protein 5 4,11E-04 4,05E-04 1,014833249 -0,006181764 0,9950677 Mdh1 malate dehydrogenase, cytoplasmic 4,14E-04 4,03E-04 1,026342463 -0,006181764 0,9950677 PSMB3 proteasome subunit beta type-3 8,35E-04 8,39E-04 1,004977644 -0,006181764 0,9950677 cct6a T-complex protein 1 subunit zeta 6,23E-04 6,18E-04 1,007948757 -0,009776924 0,99219927 CCDC147 coiled-coil domain containing 147 6,49E-04 6,41E-04 1,012570506 -0,013833024 0,9889632 LOC100046510 dynein heavy chain 8, axonemal 4,25E-04 4,30E-04 1,010951554 -0,016997724 0,98643843 4930404N11Rik uncharacterized protein C19orf71 homolog 0,001126056 0,001129616 1,003161427 -0,021431314 0,98290159 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, NDUFS4 mitochondrial 6,46E-04 6,09E-04 1,06192189 -0,023013199 0,98163974 serine/threonine-protein phosphatase 2A 65 kDa regulatory PPP2R1A subunit A alpha isoform 1,32E-04 1,34E-04 1,012259701 -0,027600845 0,97798051 ccdc108 coiled-coil domain-containing protein 108 6,55E-05 6,40E-05 1,02312963 -0,027600845 0,97798051 GALC galactocerebrosidase precursor 1,98E-04 2,07E-04 1,045020827 -0,027600845 0,97798051 Cpt1b carnitine O-palmitoyltransferase 1, muscle isoform 5,94E-04 5,77E-04 1,029187723 -0,031180169 0,97512586 BSPRY B box and SPRY domain-containing protein 5,68E-04 5,86E-04 1,031692156 -0,047815785 0,96186306

LOC100044117 pyruvate dehydrogenase protein X component, mitochondrial 0,002185123 0,00215737 1,012863995 -0,048447826 0,96135934 PPP3R2 calcineurin subunit B type 2 4,12E-04 4,08E-04 1,010847171 -0,048774916 0,96109867 cutC copper homeostasis protein cutC homolog isoform 2 5,55E-04 5,70E-04 1,025966857 -0,054337937 0,95666593

Spaca4 sperm acrosome membrane-associated protein 4 precursor 0,001723134 0,001790601 1,039153348 -0,054337937 0,95666593 Odf3b outer dense fiber protein 3B 7,01E-04 6,50E-04 1,078056551 -0,054337937 0,95666593 Gm2972 ATP synthase subunit e, mitochondrial 0,001611399 0,001616769 1,003332191 -0,054337937 0,95666593 PDIA3 protein disulfide-isomerase A3 precursor 4,02E-04 3,95E-04 1,015440491 -0,060402886 0,95183476 iron-sulfur cluster co-chaperone protein HscB, mitochondrial hscB precursor 5,71E-04 5,55E-04 1,028525332 -0,068985826 0,9450009 1700011F03Rik testis-expressed sequence 37 protein 0,001006744 0,001042771 1,035785077 -0,068985826 0,9450009 CAND1 cullin-associated NEDD8-dissociated protein 1 2,98E-04 2,97E-04 1,003912398 -0,076861817 0,93873347 uncharacterized protein C2orf47 homolog, mitochondrial 9430016H08Rik precursor 3,24E-04 3,25E-04 1,002745278 -0,086369513 0,93117268 dolichyl-diphosphooligosaccharide--protein glycosyltransferase RPN1 subunit 1 precursor 1,50E-04 1,52E-04 1,01452516 -0,086369513 0,93117268 Gm4787 a disintegrin and metalloprotease domain 4b precursor 4,00E-04 4,08E-04 1,019032126 -0,096054422 0,92347735 TTC25 tetratricopeptide repeat protein 25 6,40E-04 6,00E-04 1,065037513 -0,099962145 0,92037438 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA9 subunit 9, mitochondrial precursor 0,001325477 0,00126258 1,049815971 -0,102737492 0,91817132 BC048562 uncharacterized protein LOC434439 8,63E-04 8,32E-04 1,037773079 -0,103289078 0,91773354 TSSK4 testis-specific serine/threonine-protein kinase 4 isoform 1 7,01E-04 7,07E-04 1,009867642 -0,107307406 0,91454511 snx3 sorting nexin-3 6,96E-05 7,41E-05 1,06522119 -0,107422218 0,91445403

Abca15 ATP-binding cassette transporter sub-family A member 15 1,24E-05 1,39E-05 1,118144661 -0,107422218 0,91445403 GLTPD1 glycolipid transfer protein domain-containing protein 1 7,48E-05 8,01E-05 1,071015191 -0,107422218 0,91445403 SVOP synaptic vesicle 2-related protein 4,46E-05 4,78E-05 1,071877838 -0,107422218 0,91445403 Gm7173 uncharacterized protein LOC636104 1,62E-05 1,78E-05 1,099292073 -0,107422218 0,91445403 trappc3 trafficking protein particle complex subunit 3 7,31E-05 7,68E-05 1,051193354 -0,107422218 0,91445403 LMNB1 lamin-B1 1,89E-05 2,05E-05 1,086125673 -0,107422218 0,91445403 FANK1 fibronectin type 3 and ankyrin repeat domains 1 protein 2,72E-04 2,85E-04 1,045812974 -0,119198713 0,90511793 Gm12906 mitochondrial import receptor subunit TOM22 homolog 0,001093137 0,001030579 1,060702121 -0,119198713 0,90511793 SLC30A9 zinc transporter 9 4,15E-05 4,47E-05 1,078866051 -0,131566083 0,89532751 ARPC4 actin-related protein 2/3 complex subunit 4 isoform 1 1,27E-04 1,38E-04 1,084748962 -0,131566083 0,89532751 Gm6242 myosin regulatory light chain 12B 1,24E-04 1,23E-04 1,012125759 -0,131566083 0,89532751 COASY bifunctional coenzyme A synthase precursor 6,43E-05 7,13E-05 1,109256442 -0,131566083 0,89532751 ATP-binding cassette sub-family B member 8, mitochondrial abcb8 precursor 3,13E-05 3,54E-05 1,129680812 -0,131566083 0,89532751 rps9 40S ribosomal protein S9 8,30E-05 8,54E-05 1,02929821 -0,131566083 0,89532751 dolichyl-diphosphooligosaccharide--protein glycosyltransferase Rpn2 subunit 2 precursor 5,01E-05 5,67E-05 1,131231928 -0,131566083 0,89532751 FAF1 FAS-associated factor 1 4,09E-05 4,54E-05 1,110321436 -0,131566083 0,89532751 aldh1a1 retinal dehydrogenase 1 4,36E-05 4,74E-05 1,087401807 -0,131566083 0,89532751 Wfdc16 WAP four-disulfide core domain 16 4,40E-04 4,45E-04 1,012874838 -0,131566083 0,89532751

Slco6b1 solute carrier organic anion transporter family, member 6b1 5,09E-05 5,71E-05 1,122598704 -0,131566083 0,89532751 GDE1 glycerophosphodiester phosphodiesterase 1 7,47E-05 8,23E-05 1,102186792 -0,131566083 0,89532751 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia lyrm1 LYR motif-containing protein 1 1,69E-04 1,91E-04 1,132673366 -0,131566083 0,89532751 Crisp2 cysteine-rich secretory protein 2 precursor 1,15E-04 1,20E-04 1,046182703 -0,131566083 0,89532751 sar1a GTP-binding protein SAR1a 1,10E-04 1,18E-04 1,072463524 -0,131566083 0,89532751 SPAG16 sperm-associated antigen 16 protein isoform 1 2,26E-04 2,26E-04 1,00161543 -0,134233729 0,89321776 DNALI1 axonemal dynein light intermediate polypeptide 1 0,002401976 0,002325986 1,032669777 -0,13965646 0,88893143 succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, suclg1 mitochondrial precursor 0,00943364 0,00893407 1,05591746 -0,14277188 0,88647035 glb1l beta-galactosidase-1-like protein precursor 2,25E-04 2,34E-04 1,041355329 -0,148503721 0,88194525 ATP6V1D V-type proton ATPase subunit D 1,02E-04 1,15E-04 1,134146047 -0,151920897 0,87924933 gpX3 glutathione peroxidase 3 precursor 1,38E-04 1,57E-04 1,143318611 -0,151920897 0,87924933 Abca14 ATP-binding cassette, sub-family A (ABC1), member 14 2,13E-05 2,33E-05 1,093259716 -0,151920897 0,87924933 ube4a ubiquitin conjugation factor E4 A 4,48E-05 5,01E-05 1,117048323 -0,151920897 0,87924933 Ubl4 ubiquitin-like protein 4A 1,61E-04 1,61E-04 1,000915473 -0,151920897 0,87924933 Krt16 keratin, type I cytoskeletal 16 6,14E-05 7,10E-05 1,155402577 -0,151920897 0,87924933 Gars glycine--tRNA ligase 3,65E-05 3,93E-05 1,074846011 -0,151920897 0,87924933 guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit gnb2 beta-2 9,31E-05 9,83E-05 1,05566489 -0,151920897 0,87924933 RAB10 ras-related protein Rab-10 1,07E-04 1,21E-04 1,123200824 -0,151920897 0,87924933 PREDICTED: cytochrome c oxidase subunit 7C, Cox7c mitochondrial-like 4,47E-04 4,56E-04 1,022066586 -0,151920897 0,87924933 Gm8219 mitochondrial pyruvate carrier 1 2,80E-04 3,18E-04 1,134558903 -0,151920897 0,87924933 isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial idh3a precursor 0,001598533 0,001611437 1,008072661 -0,159123407 0,87357165 ATP5J2 ATP synthase subunit f, mitochondrial 0,001238591 0,001309186 1,056996288 -0,162098673 0,87122815 LOC100044663 apolipoprotein O isoform 1 0,001398155 0,001488614 1,064698971 -0,168597731 0,86611307 Gm1060 dynein regulatory complex protein 1 3,09E-04 3,14E-04 1,018477793 -0,168597731 0,86611307 C4bp C4b-binding protein precursor 9,47E-05 9,84E-05 1,039355113 -0,16985437 0,86512467 NADH dehydrogenase [ubiquinone] flavoprotein 3, NDUFV3 mitochondrial isoform 2 2,69E-04 2,95E-04 1,094637297 -0,16985437 0,86512467 CATSPER1 cation channel sperm-associated protein 1 1,30E-04 1,43E-04 1,100781423 -0,16985437 0,86512467 mitochondrial import inner membrane translocase subunit Gm10171 Tim23 2,64E-04 3,00E-04 1,136483505 -0,16985437 0,86512467 Gm9840 E3 ubiquitin-protein ligase RBX1 4,75E-04 5,03E-04 1,057255894 -0,16985437 0,86512467 LOC675857 transitional endoplasmic reticulum ATPase 0,001460363 0,001488977 1,019593534 -0,178648718 0,85821354 Rsph10a PREDICTED: radial spoke head 10 homolog B 5,79E-05 6,36E-05 1,09922251 -0,186067941 0,85239148 2200002J24Rik uncharacterized protein LOC69147 4,33E-04 5,13E-04 1,184648118 -0,186067941 0,85239148 FXN frataxin, mitochondrial precursor 1,91E-04 2,08E-04 1,089354018 -0,186067941 0,85239148 succinate dehydrogenase cytochrome b560 subunit, sdhC mitochondrial precursor 3,75E-04 4,30E-04 1,145445918 -0,186067941 0,85239148 lrrc23 leucine-rich repeat-containing protein 23 4,42E-04 4,38E-04 1,008351004 -0,18755059 0,85122896 rab5c ras-related protein Rab-5C 0,001053306 0,001075005 1,020600102 -0,189865484 0,84941454 ppiB peptidyl-prolyl cis-trans isomerase B precursor 5,58E-04 5,71E-04 1,022555548 -0,199523616 0,84185317 CISD1 CDGSH iron-sulfur domain-containing protein 1 0,002194542 0,001923299 1,141030045 -0,20008521 0,84141394 vps25 vacuolar protein-sorting-associated protein 25 2,70E-04 2,81E-04 1,041201639 -0,200978291 0,84071555 RALA ras-related protein Ral-A precursor 2,55E-04 2,49E-04 1,023751437 -0,200978291 0,84071555 SCP2 non-specific lipid-transfer protein 1,10E-04 1,12E-04 1,013749222 -0,200978291 0,84071555 psmc1 26S protease regulatory subunit 4 1,01E-04 1,06E-04 1,049908279 -0,200978291 0,84071555 ATP8A2 probable phospholipid-transporting ATPase IB 4,99E-05 5,68E-05 1,13960985 -0,200978291 0,84071555 NME4 nucleoside diphosphate kinase, mitochondrial precursor 3,57E-04 3,80E-04 1,064336505 -0,200978291 0,84071555

DNAJA3 dnaJ homolog subfamily A member 3, mitochondrial isoform 1 1,10E-04 1,20E-04 1,083891585 -0,21485691 0,82987888 1700080E11Rik uncharacterized protein LOC73532 2,26E-04 2,53E-04 1,117072362 -0,21485691 0,82987888 ARF2 ADP-ribosylation factor 2 3,14E-04 3,39E-04 1,08148114 -0,21485691 0,82987888 Naglu alpha-N-acetylglucosaminidase precursor 9,03E-04 9,36E-04 1,036620967 -0,216236415 0,82880347 KRT1 keratin, type II cytoskeletal 1 6,83E-04 7,26E-04 1,062195773 -0,219779631 0,82604278 1700001P01Rik uncharacterized protein C17orf98 homolog 4,26E-04 4,32E-04 1,0140593 -0,227892372 0,81972991 Tceb2 transcription elongation factor B polypeptide 2 5,07E-04 6,04E-04 1,191363433 -0,227892372 0,81972991 cetn1 centrin-1 3,27E-04 3,49E-04 1,067772685 -0,227892372 0,81972991 ak1 adenylate kinase isoenzyme 1 isoform 1 0,002974642 0,003045266 1,023742054 -0,229648204 0,81836515 WDR64 WD repeat-containing protein 64 1,75E-04 1,89E-04 1,084156175 -0,232962668 0,81579039 Prdx6 peroxiredoxin-6 5,83E-04 6,07E-04 1,041454659 -0,232962668 0,81579039

COX5A cytochrome c oxidase subunit 5A, mitochondrial precursor 0,003235071 0,003036028 1,065560362 -0,238504791 0,8114896 Wdr96 WD repeat domain 96 1,97E-04 2,05E-04 1,041959106 -0,238574863 0,81143526 CCT6B T-complex protein 1 subunit zeta-2 1,29E-04 1,40E-04 1,088446071 -0,240221977 0,81015818 A330021E22Rik uncharacterized protein C7orf63 homolog 8,28E-05 9,34E-05 1,127591319 -0,240221977 0,81015818 Uqcrh cytochrome b-c1 complex subunit 6, mitochondrial 7,57E-04 7,83E-04 1,034820963 -0,251949373 0,8010802 Gm6293 cytochrome b-c1 complex subunit 9 0,001375396 0,001484117 1,079047037 -0,251949373 0,8010802 Pdzk1 Na(+)/H(+) exchange regulatory cofactor NHE-RF3 1,29E-04 1,38E-04 1,067560533 -0,263155085 0,79243105 pcmt1 protein-L-isoaspartate(D-aspartate) O-methyltransferase 3,17E-04 3,31E-04 1,046067387 -0,263155085 0,79243105 lipe hormone-sensitive lipase isoform 1 3,25E-04 3,36E-04 1,032580364 -0,272933259 0,78490452

COX2 cytochrome c oxidase subunit II [Mus musculus castaneus] 0,007415808 0,008015447 1,080859536 -0,273574511 0,78441162 1700007K13Rik UPF0691 protein C9orf116 homolog 7,96E-04 8,24E-04 1,034779995 -0,273903147 0,78415905 ppp2r4 serine/threonine-protein phosphatase 2A activator 2,69E-04 3,00E-04 1,117417176 -0,273903147 0,78415905 LYZL6 lysozyme-like protein 6 precursor 0,001504172 0,001485757 1,012394133 -0,273903147 0,78415905 1700019N19Rik uncharacterized protein C10orf82 homolog 0,001223709 0,001282816 1,0483018 -0,282258891 0,777745 Ttc39d tetratricopeptide repeat domain 39D 4,26E-04 4,45E-04 1,04613366 -0,282258891 0,777745 PPT1 palmitoyl-protein thioesterase 1 precursor 4,09E-04 4,50E-04 1,100717369 -0,28424548 0,77622226 PSMB6 proteasome subunit beta type-6 precursor 4,42E-04 5,02E-04 1,135696808 -0,28424548 0,77622226 PSMD13 26S proteasome non-ATPase regulatory subunit 13 2,56E-04 2,71E-04 1,059511974 -0,294224872 0,76858608 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, Ndufs8 mitochondrial 9,78E-04 0,001089685 1,11472695 -0,30023223 0,76400002 CCDC39 coiled-coil domain-containing protein 39 3,03E-04 3,16E-04 1,040845556 -0,306490377 0,75923131 ATP5B ATP synthase subunit beta, mitochondrial precursor 0,009936516 0,010215866 1,028113394 -0,312886968 0,75436654 Gm2903 10 kDa heat shock protein, mitochondrial 5,62E-04 6,05E-04 1,076182781 -0,313232358 0,75410414 Ak2 adenylate kinase 2, mitochondrial isoform b 0,001984876 0,00185879 1,067832179 -0,313317428 0,75403951 Ubxn11 UBX domain-containing protein 11 2,66E-04 2,90E-04 1,088323669 -0,322316557 0,74721289 DPP3 dipeptidyl peptidase 3 1,81E-04 1,95E-04 1,07792916 -0,322316557 0,74721289 Dnahc7a dynein, axonemal, heavy chain 7B 6,32E-05 6,81E-05 1,078314007 -0,325698715 0,74465232 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia Gm8566 superoxide dismutase [Cu-Zn] 7,71E-04 8,46E-04 1,098482065 -0,331151989 0,74052968 ftmt ferritin, mitochondrial precursor 6,13E-04 7,18E-04 1,171992749 -0,348151781 0,7277262 mmel1 membrane metallo-endopeptidase-like 1 2,40E-04 2,55E-04 1,062566517 -0,356348158 0,72157985 CD109 CD109 antigen precursor 6,49E-04 6,93E-04 1,067519685 -0,356748961 0,72127975 TPPP2 tubulin polymerization-promoting protein family member 2 6,16E-04 6,50E-04 1,055488449 -0,364360509 0,71558882 4933411K16Rik uncharacterized protein C10orf62 homolog 8,77E-04 9,01E-04 1,027066837 -0,364360509 0,71558882 Nnt NAD(P) transhydrogenase, mitochondrial precursor 2,47E-04 2,72E-04 1,101002352 -0,372200718 0,70974341 mycophenolic acid acyl-glucuronide esterase, mitochondrial ABHD10 isoform 1 precursor 8,61E-04 9,62E-04 1,117947363 -0,379551989 0,70427801 odf3 outer dense fiber protein 3 0,004100466 0,003834831 1,069268844 -0,408091027 0,68320685 CAPZA3 F-actin-capping protein subunit alpha-3 6,51E-04 7,55E-04 1,159623204 -0,416157226 0,67729496 Nit1 nitrilase homolog 1 isoform 2 7,64E-04 8,73E-04 1,142193851 -0,423040419 0,67226577 ACRBP acrosin-binding protein isoform 1 precursor 0,003030314 0,002872778 1,054837636 -0,436766276 0,66228086 Dbil5 diazepam-binding inhibitor-like 5 0,001811525 0,001896206 1,046745556 -0,443050356 0,65772931 Defb22 beta-defensin 22 precursor 0,001538171 0,001486792 1,034556986 -0,443050356 0,65772931 PSMA1 proteasome subunit alpha type-1 0,001014368 0,00110216 1,086548717 -0,449522939 0,65305446 1700026D08Rik uncharacterized protein C15orf26 homolog 9,19E-04 9,99E-04 1,086417642 -0,455903883 0,6484591 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit NDUFB3 3 4,97E-04 6,78E-04 1,36296268 -0,463905811 0,64271523 PSMD8 26S proteasome non-ATPase regulatory subunit 8 2,11E-04 2,48E-04 1,173120245 -0,463905811 0,64271523 1700007G11Rik uncharacterized protein C4orf22 homolog 3,32E-04 4,05E-04 1,218626041 -0,463905811 0,64271523 Dguok deoxyguanosine kinase, mitochondrial isoform 1 2,18E-04 2,68E-04 1,231727513 -0,464375686 0,64237861 psmc3 26S protease regulatory subunit 6A 1,35E-04 1,64E-04 1,215950969 -0,464710092 0,64213908 C1orf101 PREDICTED: uncharacterized protein C1orf101-like 1,05E-04 1,29E-04 1,224282512 -0,464710092 0,64213908 Odf3l1 outer dense fiber protein 3-like protein 1 3,26E-04 3,96E-04 1,217112485 -0,466451305 0,64089249 slc16a7 monocarboxylate transporter 2 5,38E-04 6,44E-04 1,19570498 -0,466451305 0,64089249 mitochondrial import inner membrane translocase subunit TIMM17B Tim17-B 3,32E-04 4,09E-04 1,231313863 -0,466623112 0,64076955 Lipe PREDICTED: hormone-sensitive lipase-like 2,39E-04 2,99E-04 1,250192644 -0,466623112 0,64076955 4930504O13Rik uncharacterized protein LOC403200 precursor 3,56E-04 4,18E-04 1,175401459 -0,466623112 0,64076955 LYZL4 lysozyme-like protein 4 precursor 6,78E-04 7,35E-04 1,083907079 -0,468895332 0,63914445 Ado 2-aminoethanethiol dioxygenase 3,57E-04 4,32E-04 1,209230867 -0,468895332 0,63914445 Dnalc4 dynein light chain 4, axonemal 3,56E-04 4,53E-04 1,27227425 -0,471433916 0,6373309 rdh11 retinol dehydrogenase 11 precursor 1,59E-04 1,90E-04 1,19842925 -0,471433916 0,6373309 TBL1XR1 F-box-like/WD repeat-containing protein TBL1XR1 1,14E-04 1,43E-04 1,250992888 -0,471433916 0,6373309 PSMD14 26S proteasome non-ATPase regulatory subunit 14 1,95E-04 2,71E-04 1,388579441 -0,471433916 0,6373309 PPIL6 peptidyl-prolyl cis-trans isomerase-like 6 4,16E-04 4,77E-04 1,146626385 -0,471874826 0,63701614 LAP3 cytosol aminopeptidase 0,001714935 0,001729692 1,008605095 -0,473353021 0,63596134

Ssr4 translocon-associated protein subunit delta isoform 1 precursor 2,93E-04 4,04E-04 1,379757627 -0,480107625 0,63115087 GANAB neutral alpha-glucosidase AB 4,80E-05 6,06E-05 1,263998357 -0,480107625 0,63115087 SPINK2 serine protease inhibitor Kazal-type 2 precursor 4,01E-04 5,12E-04 1,277612318 -0,480107625 0,63115087 Hmox2 heme oxygenase 2 3,45E-04 3,95E-04 1,143788206 -0,48294653 0,6291337 Smcp sperm mitochondrial-associated cysteine-rich protein 0,001613096 0,00168175 1,042560274 -0,48294653 0,6291337 BC061194 uncharacterized protein LOC381350 3,14E-04 3,58E-04 1,138484534 -0,491429516 0,62312269 Fam82b regulator of microtubule dynamics protein 1 4,17E-04 4,80E-04 1,153374795 -0,491429516 0,62312269 glrx5 glutaredoxin-related protein 5, mitochondrial 2,10E-04 2,85E-04 1,354767701 -0,494965542 0,62062444 BLVRB flavin reductase (NADPH) 1,42E-04 2,06E-04 1,450515671 -0,494965542 0,62062444 glycerophosphodiester phosphodiesterase domain-containing gdpd1 protein 1 8,20E-05 1,13E-04 1,372615879 -0,494965542 0,62062444 SPATA24 spermatogenesis-associated protein 24 isoform 1 1,55E-04 1,97E-04 1,265243222 -0,494965542 0,62062444 glyctk glycerate kinase 8,37E-05 1,16E-04 1,381598188 -0,494965542 0,62062444 Antxrl anthrax toxin receptor-like precursor 6,16E-05 8,32E-05 1,350871435 -0,494965542 0,62062444 Lypla1 acyl-protein thioesterase 1 1,66E-04 2,12E-04 1,271487504 -0,494965542 0,62062444 GCLM glutamate--cysteine ligase regulatory subunit 1,78E-04 2,33E-04 1,31275972 -0,494965542 0,62062444 tmem38b trimeric intracellular cation channel type B 1,22E-04 1,72E-04 1,411934281 -0,494965542 0,62062444 psmb7 proteasome subunit beta type-7 precursor 1,14E-04 1,48E-04 1,296013126 -0,494965542 0,62062444

DHRS7B dehydrogenase/reductase SDR family member 7B isoform 2 4,25E-04 4,71E-04 1,10861943 -0,495871799 0,61998486 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex ndufa6 subunit 6 0,001093545 0,001247727 1,140992674 -0,514409898 0,60696544 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit Ndufb5 5, mitochondrial precursor 8,41E-04 8,55E-04 1,01709329 -0,514409898 0,60696544 2310061I04Rik uncharacterized protein LOC69662 9,93E-05 1,43E-04 1,440124146 -0,520630369 0,60262429 thioredoxin-dependent peroxide reductase, mitochondrial prdx3 precursor 9,72E-05 1,39E-04 1,431641635 -0,520630369 0,60262429

Cdc42 cell division control protein 42 homolog isoform 1 precursor 1,06E-04 1,57E-04 1,472751916 -0,520630369 0,60262429 PREDICTED: mitochondrial import inner membrane Tim14 translocase subunit TIM14-like 1,34E-04 1,95E-04 1,451324445 -0,520630369 0,60262429 9230107M04Rik binder of sperm protein homolog 2 precursor 2,22E-04 3,00E-04 1,353119331 -0,520630369 0,60262429 4933401F05Rik serine protease 55 precursor 1,69E-04 2,56E-04 1,516740612 -0,520630369 0,60262429 Gm1974 translationally-controlled tumor protein 1,73E-04 2,52E-04 1,458109808 -0,520630369 0,60262429 msto1 protein misato homolog 1 4,25E-05 6,10E-05 1,433592658 -0,520630369 0,60262429

PMPCA mitochondrial-processing peptidase subunit alpha precursor 4,60E-05 6,57E-05 1,427934438 -0,520630369 0,60262429 ube2v2 ubiquitin-conjugating enzyme E2 variant 2 isoform 1 1,13E-04 1,53E-04 1,353510612 -0,520630369 0,60262429 SLC44A5 choline transporter-like protein 5 6,72E-04 7,75E-04 1,152446193 -0,520975417 0,6023839 NUP155 nuclear pore complex protein Nup155 1,98E-04 2,33E-04 1,181102142 -0,523925739 0,60033019 Gm9047 uncharacterized protein LOC668210 8,33E-04 9,20E-04 1,105686051 -0,523925739 0,60033019 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA5 subunit 5 0,001397364 0,0016118 1,153457457 -0,528709338 0,5970071 wdr16 WD repeat-containing protein 16 0,001084457 0,001169221 1,078163318 -0,53040363 0,59583211 CCDC63 coiled-coil domain-containing protein 63 0,001076706 0,001122433 1,042469144 -0,54032273 0,58897449 Gm5928 ubiquitin-40S ribosomal protein S27a precursor 0,003168195 0,003394076 1,071296521 -0,542077071 0,58776541 HTRA2 serine protease HTRA2, mitochondrial 9,54E-04 0,001048764 1,099064452 -0,548013181 0,58368285 TPI1 triosephosphate isomerase 0,001421865 0,001564426 1,100263085 -0,558921087 0,57621558 EF-hand calcium-binding domain-containing protein 6 isoform EFCAB6 1 5,64E-04 5,86E-04 1,039649559 -0,562990142 0,5734416 Krt42 keratin, type I cytoskeletal 42 3,39E-05 5,81E-05 1,711411115 -0,567971291 0,57005446 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia calcium-binding tyrosine phosphorylation-regulated protein Cabyr isoform c 4,72E-05 8,22E-05 1,740970933 -0,567971291 0,57005446 ODF4 outer dense fiber protein 4 9,02E-05 1,61E-04 1,781243511 -0,567971291 0,57005446 - predicted gene 6760 9,88E-05 1,64E-04 1,66521427 -0,567971291 0,57005446 MYCBPAP MYCBP-associated protein 2,32E-05 3,63E-05 1,568620882 -0,567971291 0,57005446 - predicted gene 11780 8,33E-05 1,32E-04 1,588812853 -0,567971291 0,57005446 LOC100046125 ras-related protein Rab-5A 6,19E-05 1,02E-04 1,649879971 -0,567971291 0,57005446 Gm595 uncharacterized protein LOC209005 2,06E-05 3,37E-05 1,635787291 -0,567971291 0,57005446 RANBP17 ran-binding protein 17 4,66E-04 5,08E-04 1,090328185 -0,583774049 0,55937234 Gm2199 mitochondrial carrier homolog 2 0,001167426 0,001319906 1,130611871 -0,590282138 0,55500151 PGAM2 phosphoglycerate mutase 2 0,00470782 0,004839845 1,028043885 -0,601028453 0,54782104 Dnaic1 dynein intermediate chain 1, axonemal 8,56E-04 9,75E-04 1,138368805 -0,603049644 0,54647566 EFHC2 EF-hand domain-containing family member C2 6,45E-04 6,87E-04 1,065098241 -0,622142455 0,5338482

Gm7591 voltage-dependent anion-selective channel protein 3 isoform 2 0,001063872 0,001127116 1,059447614 -0,639739326 0,52234208 Hist1h4c histone H4 9,15E-04 0,001063786 1,16305084 -0,656122213 0,51174549 PROM1 prominin-1 isoform s1 precursor 2,04E-04 2,57E-04 1,25980647 -0,656122213 0,51174549 TSNAXIP1 translin-associated factor X-interacting protein 1 1,68E-04 2,10E-04 1,247084173 -0,656502921 0,51150059 calcium-binding tyrosine phosphorylation-regulated protein Cabyr isoform a 4,26E-04 5,49E-04 1,288325736 -0,656780421 0,51132212 LOC100045699 electron transfer flavoprotein subunit beta 2,86E-04 3,58E-04 1,252111819 -0,657994892 0,51054141 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex Ndufa7 subunit 7 8,97E-04 0,001048272 1,168621022 -0,659735192 0,50942378 Tssk2 testis-specific serine/threonine-protein kinase 2 2,39E-04 2,90E-04 1,210750886 -0,659952633 0,50928423 1700093K21Rik uncharacterized protein C2orf74 homolog 6,04E-04 8,15E-04 1,349199786 -0,659952633 0,50928423 spermatogenesis-associated protein 19, mitochondrial SPATA19 precursor 6,14E-04 7,75E-04 1,262063024 -0,659952633 0,50928423 hydroxyacylglutathione hydrolase, mitochondrial isoform 1 HAGH precursor 3,54E-04 4,19E-04 1,183915966 -0,659952633 0,50928423 Gm9234 peptidyl-prolyl cis-trans isomerase A 4,57E-04 5,73E-04 1,254413622 -0,659952633 0,50928423 Atp6v1a V-type proton ATPase catalytic subunit A 2,71E-04 3,39E-04 1,250380993 -0,663198373 0,50720351 SPACA5 sperm acrosome-associated protein 5 precursor 6,36E-04 7,73E-04 1,215460064 -0,666750178 0,50493172 POLDIP2 polymerase delta-interacting protein 2 2,21E-04 2,89E-04 1,311278206 -0,666750178 0,50493172

Gm711 probable inactive protein kinase-like protein SgK071 homolog 2,12E-04 2,79E-04 1,314782211 -0,666750178 0,50493172 Arl3 ADP-ribosylation factor-like protein 3 4,01E-04 5,22E-04 1,301280551 -0,666750178 0,50493172 EFHB EF-hand domain-containing family member B 2,49E-04 2,84E-04 1,140504637 -0,672223735 0,50144127 GAS8 growth arrest-specific protein 8 3,74E-04 4,21E-04 1,127183104 -0,677483107 0,49809948 DNAJB3 dnaJ homolog subfamily B member 3 2,63E-04 3,43E-04 1,305617868 -0,688123503 0,49137501 NHEDC1 sodium/hydrogen exchanger 9B1 6,67E-04 8,02E-04 1,203163159 -0,68899432 0,49082684 PRSS21 testisin precursor 0,003042901 0,00336054 1,104387111 -0,694572177 0,48732345 Gm5429 uncharacterized protein C7orf72 homolog 4,82E-04 7,27E-04 1,507846507 -0,700017466 0,4839164 Slc25a21 mitochondrial 2-oxodicarboxylate carrier isoform 1 1,65E-04 2,15E-04 1,300363163 -0,700017466 0,4839164 pyruvate dehydrogenase E1 component subunit beta, Gm6123 mitochondrial precursor 0,007517306 0,008671776 1,153575032 -0,705003654 0,48080796 LYZL1 lysozyme-like protein 1 precursor 4,68E-04 6,98E-04 1,492087094 -0,715616347 0,47422828 GHITM growth hormone-inducible transmembrane protein 2,37E-04 3,17E-04 1,337343675 -0,715616347 0,47422828 Gm7655 isopentenyl-diphosphate Delta-isomerase 1 1,64E-04 2,31E-04 1,409556894 -0,715616347 0,47422828 STUB1 STIP1 homology and U box-containing protein 1 1,21E-04 1,64E-04 1,361117513 -0,715616347 0,47422828 Mdh1b putative malate dehydrogenase 1B 1,05E-04 1,56E-04 1,478666245 -0,736307466 0,4615436 KRT18 keratin, type I cytoskeletal 18 8,04E-05 1,21E-04 1,500701486 -0,736307466 0,4615436 LYRM4 LYR motif-containing protein 4 3,96E-04 5,57E-04 1,407667891 -0,736307466 0,4615436 Gm10078 28S ribosomal protein S36, mitochondrial isoform 1 6,71E-04 8,29E-04 1,235960495 -0,764283941 0,44469805 putative transferase CAF17 homolog, mitochondrial isoform 1 A230051G13Rik precursor 1,25E-04 1,90E-04 1,525830896 -0,764283941 0,44469805 Gm14316 serine protease inhibitor, Kunitz type 5 precursor 3,40E-04 4,54E-04 1,337451603 -0,764283941 0,44469805 gm2a ganglioside GM2 activator precursor 2,54E-04 3,68E-04 1,452666967 -0,764283941 0,44469805 ASB9 ankyrin repeat and SOCS box protein 9 2,00E-04 3,19E-04 1,59469288 -0,764283941 0,44469805 LOC100047501 40S ribosomal protein S16 1,35E-04 2,30E-04 1,708153129 -0,803252134 0,42182902 NIPSNAP1 protein NipSnap homolog 1 1,01E-04 1,67E-04 1,649356384 -0,803252134 0,42182902 ANKRD45 ankyrin repeat domain-containing protein 45 1,07E-04 1,75E-04 1,630014823 -0,803252134 0,42182902 YWHAG 14-3-3 protein gamma 9,69E-05 1,63E-04 1,684161028 -0,803252134 0,42182902 Gm16379 macrophage migration inhibitory factor 4,02E-04 6,91E-04 1,721622931 -0,803252134 0,42182902 hydroxyacyl-coenzyme A dehydrogenase, mitochondrial hadh precursor 1,01E-04 1,74E-04 1,724474435 -0,803252134 0,42182902 leucine-rich repeat and guanylate kinase domain-containing LRGUK protein 4,39E-05 7,44E-05 1,695277265 -0,803252134 0,42182902 Car2 carbonic anhydrase 2 8,87E-04 0,001001324 1,12947469 -0,805350571 0,42061741 GSTO2 glutathione S-transferase omega-2 0,001046446 0,001211389 1,157621858 -0,806934065 0,41970447 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit Ndufb7 7 0,001313313 0,001570406 1,195759282 -0,809394823 0,41828808 Fhl4 four and a half LIM domains 4 8,94E-04 9,82E-04 1,097980977 -0,812347111 0,41659249 Slc25a11 mitochondrial 2-oxoglutarate/malate carrier protein 3,79E-04 4,74E-04 1,252212052 -0,813270166 0,41606318 Cox6b2 cytochrome c oxidase subunit 6B2 isoform 2 0,001393207 0,001974845 1,417481482 -0,813270166 0,41606318 LOC100046120 clusterin precursor 7,03E-04 8,33E-04 1,184056957 -0,81571022 0,41466589 Lrrc18 leucine-rich repeat-containing protein 18 isoform 1 4,36E-04 4,95E-04 1,135023108 -0,816650236 0,41412834 SLC8A2 sodium/calcium exchanger 2 precursor 2,31E-04 2,88E-04 1,248952123 -0,816650236 0,41412834 Gm7204 peroxiredoxin-1 5,19E-04 6,16E-04 1,186265334 -0,816650236 0,41412834 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, Gm12251 mitochondrial precursor 0,002455362 0,00263213 1,071992909 -0,819014537 0,41277812 1700020C11Rik mitochondrial fission process protein 1 6,55E-04 8,64E-04 1,317616222 -0,831659331 0,40560126 - PREDICTED: uncharacterized protein C17orf47 homolog 7,06E-04 8,09E-04 1,145797632 -0,834417389 0,40404582 Ywhaz 14-3-3 protein zeta/delta isoform 1 0,001972862 0,002339608 1,185895438 -0,835104482 0,40365888 APOOL apolipoprotein O-like precursor 2,94E-04 3,92E-04 1,332185945 -0,83876334 0,40160213 glycerophosphodiester phosphodiesterase domain-containing Gdpd4 protein 4 3,06E-04 4,00E-04 1,305909018 -0,83876334 0,40160213 tpp2 tripeptidyl-peptidase 2 3,10E-04 3,52E-04 1,1355082 -0,841541961 0,40004439 TSSK6 testis-specific serine/threonine-protein kinase 6 3,03E-04 3,77E-04 1,244476997 -0,84724542 0,39685834 Crat carnitine O-acetyltransferase 0,004584597 0,004857197 1,05946014 -0,851879485 0,39428098 FAM154A uncharacterized protein LOC75811 9,91E-04 0,001100669 1,110169256 -0,854035915 0,39308509 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia MECR trans-2-enoyl-CoA reductase, mitochondrial precursor 0,001161125 0,001344568 1,157987699 -0,854035915 0,39308509 MAN2B2 epididymis-specific alpha-mannosidase precursor 0,001273011 0,001458717 1,14587936 -0,856733535 0,39159217 LCN12 epididymal-specific lipocalin-12 precursor 8,09E-04 0,001101436 1,36092675 -0,857380139 0,39123483 ART3 ecto-ADP-ribosyltransferase 3 precursor 7,28E-05 1,38E-04 1,897098952 -0,860074273 0,3897481 INPP1 inositol polyphosphate 1-phosphatase 6,38E-05 1,16E-04 1,819305775 -0,860074273 0,3897481 Txndc2 thioredoxin domain-containing protein 2 isoform 2 3,94E-05 7,31E-05 1,856370766 -0,860074273 0,3897481 Adcy10 adenylate cyclase type 10 1,54E-05 2,82E-05 1,837461429 -0,860074273 0,3897481 Rab7 ras-related protein Rab-7a 6,46E-05 1,23E-04 1,897381127 -0,860074273 0,3897481 Cnnm4 metal transporter CNNM4 precursor 3,14E-05 5,79E-05 1,841700855 -0,860074273 0,3897481 GSTM5 glutathione S-transferase Mu 5 0,002420019 0,002329259 1,038965272 -0,863790293 0,3877031 PTCHD3 patched domain-containing protein 3 8,93E-04 0,001054104 1,180952089 -0,864437307 0,38734771 IDH3B isocitrate dehydrogenase 3, beta subunit 0,001614965 0,001848197 1,144419368 -0,86745143 0,38569471 snap23 synaptosomal-associated protein 23 isoform a 2,81E-04 3,94E-04 1,401457668 -0,869520328 0,38456259 CCT8 T-complex protein 1 subunit theta 6,21E-04 6,84E-04 1,100205702 -0,872408574 0,38298552 GPX5 epididymal secretory glutathione peroxidase precursor 3,00E-04 4,20E-04 1,398998762 -0,884127695 0,37662728 slc25a1 solute carrier family 25, member 1 2,38E-04 3,18E-04 1,337646475 -0,884127695 0,37662728 CCDC96 coiled-coil domain-containing protein 96 1,21E-04 1,60E-04 1,316687825 -0,884127695 0,37662728 uqcrfs1 cytochrome b-c1 complex subunit Rieske, mitochondrial 0,003311531 0,003718043 1,122756283 -0,88969868 0,3736277 Arl8b ADP-ribosylation factor-like protein 8B 2,87E-04 4,22E-04 1,473612506 -0,901819338 0,36715285 PREDICTED: up-regulated during skeletal muscle growth - protein 5-like 0,001270842 0,001582726 1,245415566 -0,901819338 0,36715285 fsip2 PREDICTED: fibrous sheath-interacting protein 2 2,28E-04 2,40E-04 1,052614439 -0,903178474 0,36643118 ACO2 aconitate hydratase, mitochondrial precursor 0,004188298 0,004379714 1,04570266 -0,920988338 0,35705652 DNAJC10 dnaJ homolog subfamily C member 10 precursor 8,02E-05 1,20E-04 1,491656456 -0,923440856 0,35577751 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit NDUFB11 11, mitochondrial 3,74E-04 5,15E-04 1,375573919 -0,923440856 0,35577751

PLB1 phospholipase B1, membrane-associated isoform 1 precursor 2,17E-04 2,64E-04 1,21580772 -0,929703645 0,35252455 csnk1a1 casein kinase I isoform alpha 6,27E-04 7,83E-04 1,247486232 -0,929715207 0,35251856 AKAP3 A-kinase anchor protein 3 0,002906583 0,002800195 1,037993118 -0,932525374 0,35106508 Dnaic2 dynein intermediate chain 2, axonemal 5,92E-04 7,24E-04 1,221780023 -0,935520366 0,34952019 Uba1 ubiquitin-like modifier-activating enzyme 1 isoform 2 2,10E-04 2,69E-04 1,283519569 -0,940048032 0,34719292 MTX2 metaxin-2 9,91E-04 0,001188466 1,198726297 -0,943045802 0,34565748 alb serum albumin precursor 5,34E-04 6,04E-04 1,132267406 -0,945754138 0,344274 C030006K11Rik UPF0598 protein C8orf82 homolog isoform 1 6,28E-05 1,36E-04 2,158658289 -0,949263255 0,34248674 CCDC38 coiled-coil domain-containing protein 38 2,20E-05 4,94E-05 2,241494389 -0,949263255 0,34248674 HINT3 histidine triad nucleotide-binding protein 3 1,01E-04 2,18E-04 2,161319772 -0,949263255 0,34248674 atp6v0d1 V-type proton ATPase subunit d 1 4,17E-05 9,71E-05 2,326266067 -0,949263255 0,34248674 stx2 syntaxin-2 5,52E-05 1,14E-04 2,071102978 -0,949263255 0,34248674 RAB22A ras-related protein Rab-22A 5,70E-05 1,15E-04 2,012186885 -0,949263255 0,34248674 PRSS37 probable inactive serine protease 37 precursor 7,00E-05 1,47E-04 2,10395352 -0,949263255 0,34248674 KRT6A keratin, type II cytoskeletal 6A 3,47E-05 7,21E-05 2,078667012 -0,949263255 0,34248674 RPS25 PREDICTED: 40S ribosomal protein S25-like 8,13E-05 1,67E-04 2,051172444 -0,949263255 0,34248674 CATSPER2 cation channel sperm-associated protein 2 1,41E-04 2,14E-04 1,516270052 -0,950186049 0,34201773 BC048507 dynein light chain LC8-type 1-like 3,99E-04 6,35E-04 1,591028252 -0,950186049 0,34201773 cytochrome c oxidase subunit 7A-related protein, Cox7a2l mitochondrial isoform 1 4,83E-04 7,31E-04 1,514156272 -0,950186049 0,34201773 Gm4045 ATP synthase subunit g, mitochondrial 0,001624239 0,001990348 1,225403211 -0,955109875 0,33952214 dci enoyl-CoA delta isomerase 1, mitochondrial precursor 5,13E-04 7,02E-04 1,367304426 -0,955109875 0,33952214 Gm11582 dynein light chain 1, cytoplasmic 0,00162785 0,002058239 1,264391298 -0,966380431 0,33385385 Fam166a protein FAM166A 0,003265506 0,003907952 1,196736895 -0,970367759 0,33186321 LOC675851 NADH dehydrogenase [ubiquinone] 1 subunit C2 0,001116575 0,001451598 1,300044879 -0,973247782 0,33043017 Abca17 ATP-binding cassette, sub-family A (ABC1), member 17 1,08E-04 1,43E-04 1,321765714 -0,981092438 0,32654716 Akr1b3 aldose reductase 4,20E-04 5,33E-04 1,268114782 -0,981092438 0,32654716 vars valine--tRNA ligase 3,55E-05 5,86E-05 1,650495311 -0,983802732 0,32521252 - uncharacterized protein LOC73863 3,40E-04 5,35E-04 1,572161978 -0,983802732 0,32521252 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA3 subunit 3 5,99E-04 0,001001684 1,67360932 -0,983802732 0,32521252 disintegrin and metalloproteinase domain-containing protein 1b Adam1b precursor 2,03E-04 2,75E-04 1,351916521 -1,000328483 0,31715157 HTRA1 serine protease HTRA1 precursor 2,12E-04 2,96E-04 1,395759155 -1,025680395 0,30504226 LOC674674 farnesyl pyrophosphate synthase isoform 2 1,17E-04 2,13E-04 1,821268419 -1,026968568 0,30443527 Hrsp12 ribonuclease UK114 1,91E-04 3,49E-04 1,827109947 -1,026968568 0,30443527 Got2 aspartate aminotransferase, mitochondrial 7,36E-04 8,88E-04 1,206443893 -1,037706611 0,29940667 ACOT7 cytosolic acyl coenzyme A thioester hydrolase isoform 2 2,72E-04 3,96E-04 1,453200057 -1,041366549 0,29770546 SYNGR4 synaptogyrin-4 0,004050254 0,004416072 1,09031967 -1,044471026 0,29626752 Acox3 peroxisomal acyl-coenzyme A oxidase 3 2,97E-04 3,79E-04 1,277150437 -1,057481567 0,29029186 LOC100046727 IQ and ubiquitin-like domain-containing protein 1,05E-04 1,57E-04 1,499801123 -1,059597208 0,28932788 PPAP2A lipid phosphate phosphohydrolase 1 isoform 1 4,72E-04 7,39E-04 1,567108444 -1,059597208 0,28932788 rps3 40S ribosomal protein S3 1,97E-04 2,93E-04 1,486135928 -1,059597208 0,28932788 Dnalc1 dynein light chain 1, axonemal 8,44E-04 9,90E-04 1,173792807 -1,069017698 0,28506169 NCSTN nicastrin precursor 3,40E-04 4,54E-04 1,333624338 -1,07908467 0,28054998 DYDC1 DPY30 domain-containing protein 1 8,73E-04 0,001103999 1,265133419 -1,07908467 0,28054998 Atp5d ATP synthase subunit delta, mitochondrial precursor 6,40E-04 9,87E-04 1,543007236 -1,080923477 0,27973115 LOC100047329 40S ribosomal protein S18 1,39E-04 2,33E-04 1,674089682 -1,084031619 0,27835078 Tspan6 tetraspanin-6 1,48E-04 2,96E-04 2,008144021 -1,084031619 0,27835078 Tmem89 transmembrane protein 89 precursor 3,19E-04 6,91E-04 2,163485585 -1,084031619 0,27835078 APOA1BP NAD(P)H-hydrate epimerase precursor 5,83E-04 8,32E-04 1,427123987 -1,098843737 0,27183623 3110056O03Rik signal peptide peptidase-like 2B precursor 2,14E-04 3,36E-04 1,570074763 -1,106072336 0,26869521 YWHAE 14-3-3 protein epsilon 1,90E-04 3,02E-04 1,583427089 -1,106072336 0,26869521 THOP1 thimet oligopeptidase 8,13E-05 1,31E-04 1,611377174 -1,136025028 0,25594609 HSPA5 78 kDa glucose-regulated protein precursor 5,56E-04 6,71E-04 1,206099129 -1,137536932 0,25531389 PSMD2 26S proteasome non-ATPase regulatory subunit 2 5,59E-04 6,68E-04 1,196125848 -1,139460373 0,25451119

Dhrs4 dehydrogenase/reductase SDR family member 4 isoform 1 8,67E-04 0,001091554 1,258677079 -1,146492986 0,25159123 CYLC2 cylicin, basic protein of sperm head cytoskeleton 2 6,12E-04 7,70E-04 1,258403521 -1,152630933 0,2490619 probable isocitrate dehydrogenase [NAD] gamma 2, 4933405O20Rik mitochondrial 7,53E-04 9,35E-04 1,242621334 -1,155135891 0,24803478 dihydroorotate dehydrogenase (quinone), mitochondrial dhodh precursor 5,80E-05 1,24E-04 2,138528569 -1,162622178 0,24498283 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia SPEF2 sperm flagellar protein 2 2,62E-05 5,82E-05 2,221369636 -1,162622178 0,24498283 1600016N20Rik uncharacterized protein C11orf35 homolog 3,72E-05 8,14E-05 2,188072537 -1,162622178 0,24498283 BC051142 testis specific basic protein isoform 2 1,50E-04 3,18E-04 2,115630184 -1,162622178 0,24498283

AGPAT2 1-acyl-sn-glycerol-3-phosphate acyltransferase beta precursor 8,18E-05 1,83E-04 2,238386164 -1,162622178 0,24498283 PMM2 phosphomannomutase 2 6,07E-05 1,29E-04 2,125806256 -1,162622178 0,24498283 1700016C15Rik uncharacterized protein C1orf100 homolog 4,33E-04 7,25E-04 1,676844513 -1,172142559 0,24113983 EFCAB1 EF-hand calcium-binding domain-containing protein 1 2,19E-04 3,48E-04 1,587635805 -1,172142559 0,24113983 ddt D-dopachrome decarboxylase 6,34E-04 9,21E-04 1,453337915 -1,180372132 0,23785224 LdhA L-lactate dehydrogenase A chain isoform 2 0,001959904 0,00222475 1,135132492 -1,185598287 0,23578098 1700101E01Rik uncharacterized protein LOC329375 isoform 1 6,76E-04 9,18E-04 1,356492395 -1,186368482 0,23547681 1700009P17Rik UPF0740 protein C1orf192 homolog 9,11E-04 0,001509581 1,657297752 -1,198545653 0,23070466 vamp3 vesicle-associated membrane protein 3 0,002123402 0,002745097 1,292782617 -1,205195288 0,22812792 CCDC135 coiled-coil domain-containing protein lobo homolog 2,07E-04 2,77E-04 1,340670252 -1,212677076 0,22525331 1700001C19Rik uncharacterized protein LOC75462 isoform 2 2,99E-04 5,52E-04 1,844555547 -1,216375783 0,2238418 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit NDUFB6 6 2,94E-04 5,56E-04 1,888097186 -1,216375783 0,2238418 PREDICTED: NADH dehydrogenase [ubiquinone] 1 beta Gm3244 subcomplex subunit 4-like 7,33E-04 0,001194145 1,629225505 -1,219223591 0,22275934 1700080O16Rik uncharacterized protein LOC74279 2,99E-04 4,51E-04 1,51041023 -1,219223591 0,22275934 1700019N12Rik testis-expressed sequence 40 protein 5,47E-04 7,85E-04 1,434737463 -1,219223591 0,22275934 1700023D19Rik izumo sperm-egg fusion protein 2 precursor 9,32E-04 0,001288857 1,383588633 -1,220865558 0,22213693 Dnahc1 dynein, axonemal, heavy chain 1 1,24E-04 1,49E-04 1,200162247 -1,228387036 0,21930171 Gm2962 cytochrome b-c1 complex subunit 7 8,83E-04 0,001257562 1,423859419 -1,239675832 0,21509532 Wdr78 WD repeat-containing protein 78 1,92E-04 2,84E-04 1,475805219 -1,239675832 0,21509532 cyc1 cytochrome c1, heme protein, mitochondrial 0,003174415 0,003728242 1,17446586 -1,250676401 0,21105256 cAMP-dependent protein kinase type I-alpha regulatory PRKAR1A subunit 7,13E-04 9,20E-04 1,289457411 -1,266566384 0,20531038

TTC19 tetratricopeptide repeat protein 19, mitochondrial isoform 1 2,12E-04 3,49E-04 1,644085222 -1,270145324 0,20403287 histidine triad nucleotide-binding protein 2, mitochondrial HINT2 precursor 1,93E-04 3,57E-04 1,855701751 -1,271636642 0,20350225 echdc1 ethylmalonyl-CoA decarboxylase isoform a 1,09E-04 2,25E-04 2,05883287 -1,271636642 0,20350225 Ptgr2 prostaglandin reductase 2 isoform 1 1,28E-04 2,45E-04 1,911294483 -1,271636642 0,20350225 CALR calreticulin precursor 1,03E-04 2,00E-04 1,943379864 -1,271636642 0,20350225 FABP4 fatty acid-binding protein, adipocyte 6,68E-05 1,90E-04 2,846871097 -1,277674509 0,20136422 LOC100045772 multidrug resistance-associated protein 9 1,36E-05 3,78E-05 2,770809064 -1,277674509 0,20136422 4930588N13Rik testis-expressed sequence 26 protein 6,21E-05 1,57E-04 2,52703528 -1,277674509 0,20136422 haloacid dehalogenase-like hydrolase domain-containing Gm10784 protein 2 isoform 1 6,14E-05 1,91E-04 3,103476593 -1,277674509 0,20136422 KRT19 keratin, type I cytoskeletal 19 2,63E-05 7,33E-05 2,789689246 -1,277674509 0,20136422 Adrbk2 beta-adrenergic receptor kinase 2 isoform 1 2,08E-05 5,53E-05 2,653711877 -1,277674509 0,20136422 Abcg2 ATP-binding cassette sub-family G member 2 3,28E-05 9,47E-05 2,887368228 -1,277674509 0,20136422 PRSS1 protease, serine, 1 precursor 0,001755553 0,002100943 1,196741472 -1,279820011 0,20060844 9030607L17Rik izumo sperm-egg fusion protein 4 precursor 0,003400782 0,003770247 1,108641303 -1,288470193 0,19758234 Dynll2 dynein light chain 2, cytoplasmic 0,001210594 0,00204365 1,688137523 -1,289992643 0,19705321 FSCN3 fascin-3 1,86E-04 3,08E-04 1,657619366 -1,301804084 0,19298337 zpbp1 zona pellucida-binding protein 1 isoform 2 0,006304115 0,00710688 1,127339855 -1,30707753 0,19118638 BPGM bisphosphoglycerate mutase 7,56E-04 0,001048901 1,387366024 -1,309898012 0,19023034 4930579C15Rik equatorin precursor 0,002197992 0,002625419 1,194462614 -1,313265877 0,18909337 Dnhc12 PREDICTED: dynein heavy chain 12, axonemal 1,11E-04 1,37E-04 1,236724039 -1,31587459 0,18821614 NIPSNAP3A nipsnap homolog 3A 0,001875618 0,002472802 1,318393058 -1,336364473 0,18143017 etfA electron transfer flavoprotein subunit alpha, mitochondrial 1,51E-04 2,53E-04 1,67121797 -1,338928585 0,18059392 RAB18 ras-related protein Rab-18 isoform 2 precursor 1,99E-04 3,45E-04 1,732522112 -1,338928585 0,18059392 - PREDICTED: uncharacterized protein LOC100862214 2,34E-04 3,82E-04 1,630510791 -1,338928585 0,18059392 NUP54 nuclear pore complex protein Nup54 1,23E-04 2,23E-04 1,811646891 -1,338928585 0,18059392 SPATA20 spermatogenesis-associated protein 20 8,34E-05 1,45E-04 1,738330446 -1,338928585 0,18059392 1700013F07Rik uncharacterized protein C1orf194 homolog 1,68E-04 3,11E-04 1,851487068 -1,342499941 0,17943395 sodium/potassium-transporting ATPase subunit alpha-1 ATP1A1 precursor 3,15E-05 6,88E-05 2,186811113 -1,342499941 0,17943395 AGPAT3 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma 9,33E-05 2,06E-04 2,208526434 -1,342499941 0,17943395 SLC13A5 solute carrier family 13 member 5 1,05E-04 2,23E-04 2,119451428 -1,342499941 0,17943395 1700011E24Rik uncharacterized protein C2orf61 homolog 1,01E-04 2,17E-04 2,141501416 -1,342499941 0,17943395 wbp2nl postacrosomal sheath WW domain-binding protein 7,15E-04 0,001077479 1,507565023 -1,343922445 0,17897347 STX12 syntaxin-12 8,41E-04 0,001150961 1,36852756 -1,343922445 0,17897347 Gm11273 cytochrome c oxidase subunit 5B, mitochondrial 0,001792688 0,002139059 1,193213618 -1,358455626 0,17431915 4921511H03Rik uncharacterized protein C7orf62 homolog 4,29E-04 7,26E-04 1,691775691 -1,366230511 0,17186662 1700018L24Rik testis-expressed sequence 29 protein 6,18E-04 9,22E-04 1,490479189 -1,366230511 0,17186662 coiled-coil-helix-coiled-coil-helix domain-containing protein 3, CHCHD3 mitochondrial precursor 9,47E-04 0,001231988 1,301183604 -1,366946205 0,17164217 Tex101 testis-expressed sequence 101 protein precursor 0,001396415 0,001982425 1,419652768 -1,366946205 0,17164217 HSD17B10 3-hydroxyacyl-CoA dehydrogenase type-2 7,91E-04 0,001071851 1,355063666 -1,376646744 0,16862149 complement component 1 Q subcomponent-binding protein, c1qbp mitochondrial 7,32E-04 0,001037886 1,417711133 -1,377738473 0,16828405 Ogdhl 2-oxoglutarate dehydrogenase-like, mitochondrial 5,40E-05 9,99E-05 1,851266219 -1,382975901 0,16667226 IQCD IQ domain-containing protein D 9,03E-05 1,63E-04 1,809340369 -1,382975901 0,16667226 5730469M10Rik redox-regulatory protein FAM213A 1,98E-04 3,71E-04 1,872480504 -1,382975901 0,16667226 HSP90AB1 heat shock protein HSP 90-beta 6,49E-05 1,17E-04 1,798078502 -1,382975901 0,16667226 PSMA8 proteasome subunit alpha type-7-like 8,12E-04 0,001073877 1,322100952 -1,387729469 0,16521944 CLMN calmin isoform a 1,65E-04 2,44E-04 1,476682686 -1,391060656 0,16420703 succinate dehydrogenase [ubiquinone] iron-sulfur subunit, LOC638710 mitochondrial precursor 0,001428569 0,001645641 1,151950266 -1,392675571 0,16371792 SPAG17 sperm-associated antigen 17 1,30E-04 1,74E-04 1,33857 -1,400400892 0,1613933 Hspa1l heat shock 70 kDa protein 1-like 0,001093426 0,001316742 1,204234689 -1,407134536 0,1593875 DNAJB13 dnaJ homolog subfamily B member 13 9,56E-04 0,001218112 1,274623126 -1,422108035 0,15499489 nsdhl sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating 1,56E-04 3,12E-04 1,998905178 -1,435996412 0,15100336 Phpt1 14 kDa phosphohistidine phosphatase 2,83E-04 5,35E-04 1,891876201 -1,435996412 0,15100336 INSL6 insulin-like peptide INSL6 precursor 2,34E-04 4,61E-04 1,968011524 -1,435996412 0,15100336 LOC674583 ATP synthase-coupling factor 6, mitochondrial precursor 1,38E-04 3,62E-04 2,613607826 -1,436649898 0,1508175 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia

Pdilt protein disulfide-isomerase-like protein of the testis precursor 3,50E-05 9,05E-05 2,588070576 -1,436649898 0,1508175 PITRM1 presequence protease, mitochondrial precursor 3,43E-04 4,37E-04 1,274347072 -1,444789667 0,14851697 Atp5c1 ATP synthase subunit gamma, mitochondrial isoform a 0,002450581 0,00279509 1,140582526 -1,468466563 0,14197753

Akd1 PREDICTED: adenylate kinase domain-containing protein 1 1,00E-04 1,44E-04 1,433361564 -1,472914287 0,14077415 Ldhc L-lactate dehydrogenase C chain 0,011579181 0,012658673 1,09322701 -1,476643038 0,13977135 bsg basigin isoform 2 precursor 0,00358995 0,004154273 1,157194967 -1,480220098 0,13881452 Gm7308 calmodulin 9,30E-04 0,001316415 1,415600707 -1,485306121 0,13746275 ANKRD5 ankyrin repeat and EF-hand domain-containing protein 1 1,74E-04 2,61E-04 1,4961009 -1,498685314 0,13395529 Gm6685 cytochrome b5 domain-containing protein 1 1,78E-04 3,92E-04 2,209832069 -1,500982352 0,13336013 Gm13050 elongation factor 2 3,72E-05 8,08E-05 2,173412912 -1,500982352 0,13336013

Samd15 PREDICTED: sterile alpha motif domain-containing protein 15 5,78E-05 1,24E-04 2,155267688 -1,500982352 0,13336013 a disintegrin and metalloprotease domain 3 (cyritestin) Adam3 precursor 5,53E-05 1,19E-04 2,145567722 -1,500982352 0,13336013 RTDR1 rhabdoid tumor deletion region gene 1 isoform 1 2,47E-04 4,29E-04 1,737561 -1,502928944 0,13285737 MAOA amine oxidase [flavin-containing] A 1,88E-04 3,11E-04 1,657773324 -1,502928944 0,13285737 Psma6 proteasome subunit alpha type-6 7,28E-04 0,001059576 1,454728915 -1,531695069 0,1255977 Gm6665 glutathione S-transferase Mu 2 1,89E-04 3,49E-04 1,845356432 -1,533133817 0,12524289 PREDICTED: glyceraldehyde-3-phosphate dehydrogenase-like Gm4335 isoform 2 0,002040427 0,002434633 1,193197431 -1,540119491 0,12353123 RAB11B ras-related protein Rab-11B 0,001802474 0,002351272 1,30446948 -1,55332644 0,12034517 Tmem177 transmembrane protein 177 3,32E-04 5,88E-04 1,773667871 -1,558327966 0,11915552 PRDX2 peroxiredoxin-2 5,74E-04 8,54E-04 1,48660835 -1,564389046 0,11772623 atp2b4 plasma membrane calcium ATPase 4 isoform b 0,001207879 0,001372469 1,136263467 -1,566105305 0,11732397

MOSC2 MOSC domain-containing protein 2, mitochondrial precursor 3,85E-05 1,31E-04 3,38846608 -1,568227649 0,11682801 cytochrome c oxidase subunit 4 isoform 1, mitochondrial COX4I1 precursor 0,005429986 0,005993724 1,103819337 -1,578499933 0,1144508 iron-sulfur cluster assembly 2 homolog, mitochondrial iscA2 precursor 1,29E-04 2,90E-04 2,241374411 -1,582503219 0,11353474 Gm7795 cytochrome c oxidase subunit 6A1, mitochondrial 5,76E-04 0,00143545 2,494141235 -1,582503219 0,11353474 Canx calnexin precursor 3,06E-04 4,76E-04 1,556576759 -1,584609081 0,11305518 TEPP testis, prostate and placenta-expressed protein isoform 1 0,00121632 0,001739518 1,430148234 -1,585567485 0,11283746 ARMC4 armadillo repeat-containing protein 4 6,47E-04 7,83E-04 1,21048124 -1,611343966 0,10710477 Usp7 ubiquitin carboxyl-terminal hydrolase 7 2,17E-04 3,04E-04 1,402557873 -1,626765899 0,10378683 Fahd1 acylpyruvase FAHD1, mitochondrial 3,09E-04 6,04E-04 1,955104835 -1,628390766 0,10344205 4933400C05Rik spermatogenesis-associated protein 32 8,15E-04 0,0011809 1,448206991 -1,636525144 0,10172973 Gm16399 cytochrome c oxidase subunit 6C 0,004448522 0,005040575 1,133089875 -1,636697969 0,10169359 PGP phosphoglycolate phosphatase 1,20E-04 2,64E-04 2,197563579 -1,644267653 0,10012093 PREDICTED: NADH dehydrogenase [ubiquinone] 1 subunit LOC675851 C2-like 3,00E-04 6,42E-04 2,143272178 -1,644267653 0,10012093 psmb1 proteasome subunit beta type-1 precursor 4,74E-04 7,08E-04 1,494356327 -1,683277575 0,09232137 abhd11 alpha/beta hydrolase domain-containing protein 11 isoform 1 1,14E-04 3,02E-04 2,659691263 -1,687955613 0,09141975 Ace3 angiotensin I converting enzyme 3 precursor 0,001051958 0,001278842 1,215678205 -1,690446391 0,09094259 HSP90AA1 heat shock protein HSP 90-alpha 5,06E-04 6,69E-04 1,321817845 -1,695204696 0,0900366 acsbg2 long-chain-fatty-acid--CoA ligase ACSBG2 0,001063661 0,001302264 1,224322564 -1,69932443 0,08925807 LOC100047986 elongation factor 1-gamma 3,36E-04 5,52E-04 1,642721005 -1,704243718 0,08833556 eIF6 eukaryotic translation initiation factor 6 2,11E-04 5,42E-04 2,566940861 -1,71713522 0,08595447 dcaf7 DDB1- and CUL4-associated factor 7 1,22E-04 2,97E-04 2,432860942 -1,71713522 0,08595447 4732415M23Rik uncharacterized protein C16orf55 homolog 4,40E-04 8,65E-04 1,966989031 -1,719480473 0,08552692 GNPAT dihydroxyacetone phosphate acyltransferase 1,14E-04 2,16E-04 1,901320351 -1,757135918 0,07889464 Irgc1 interferon-inducible GTPase 5 0,001088223 0,001435365 1,318998567 -1,774907116 0,07591323 Zp3r zona pellucida sperm-binding protein 3 receptor precursor 0,002057406 0,002642884 1,284570718 -1,801658747 0,07159911 LOC100048051 multidrug and toxin extrusion protein 2 1,06E-04 2,95E-04 2,798877328 -1,806965817 0,07076761 pgam1 phosphoglycerate mutase 1 1,10E-04 3,07E-04 2,78614491 -1,806965817 0,07076761 Tmprss12 transmembrane protease serine 12 precursor 1,13E-04 3,07E-04 2,717615429 -1,806965817 0,07076761 Maai maleylacetoacetate isomerase isoform 2 8,29E-05 3,22E-04 3,881700367 -1,830278814 0,06720826 HSPA4 heat shock 70 kDa protein 4 1,57E-05 6,02E-05 3,832562083 -1,830278814 0,06720826 IMPA1 inositol monophosphatase 1 7,63E-04 0,001171277 1,53432156 -1,835738191 0,06639639 LOC631229 ADP/ATP translocase 2 2,63E-04 4,30E-04 1,636130272 -1,84153525 0,06554316 lancl1 lanC-like protein 1 1,37E-04 3,19E-04 2,336380179 -1,842510947 0,06540045 PARK7 protein DJ-1 9,94E-04 0,001475203 1,483445298 -1,847391311 0,06469045 Slc25a31 ADP/ATP translocase 4 0,002373479 0,002774344 1,168893572 -1,859178866 0,06300179 4930519F16Rik uncharacterized protein LOC75106 precursor 0,001443312 0,002122381 1,470493876 -1,859679681 0,06293086 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex Ndufa13 subunit 13 0,001577929 0,002366145 1,49952552 -1,86005036 0,0628784 AA467197 normal mucosa of esophagus-specific gene 1 protein 0,004605966 0,005404045 1,173270761 -1,86005036 0,0628784 Dnah7 PREDICTED: dynein heavy chain 7, axonemal 4,11E-05 6,68E-05 1,622797749 -1,865102137 0,06216709 PREDICTED: orphan sodium- and chloride-dependent Ntt5 neurotransmitter transporter NTT5 2,92E-04 5,79E-04 1,980463755 -1,877797734 0,06040884 psma5 proteasome subunit alpha type-5 7,51E-04 0,001203565 1,603100739 -1,884203519 0,05953745 Sypl synaptophysin-like protein 1 isoform 1 7,75E-04 0,001463156 1,887595365 -1,893723406 0,05826173 APOE apolipoprotein E precursor 1,63E-04 3,68E-04 2,25971294 -1,898691868 0,057605 Acsl6 long-chain-fatty-acid--CoA ligase 6 isoform 1 7,85E-05 1,82E-04 2,314649429 -1,898691868 0,057605 RSPH6A radial spoke head protein 6 homolog A isoform 1 0,001295658 0,001883812 1,453942357 -1,908633499 0,05630939 CLTC clathrin heavy chain 1 5,20E-05 1,02E-04 1,96356914 -1,91811028 0,05509703 NRD1 nardilysin precursor 2,29E-05 7,49E-05 3,266021369 -1,920715942 0,05476753 LRP2BP LRP2-binding protein 6,66E-05 2,12E-04 3,180911219 -1,920715942 0,05476753 1700028P14Rik uncharacterized protein C9orf135 homolog 3,09E-04 5,71E-04 1,847031776 -1,956003113 0,05046479 pgls 6-phosphogluconolactonase 2,00E-04 4,83E-04 2,41552157 -1,960195298 0,04997297 STXBP2 syntaxin-binding protein 2 7,92E-05 1,73E-04 2,186802643 -1,960195298 0,04997297 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit Gm9050 11, mitochondrial-like 4,10E-04 9,72E-04 2,370614287 -1,960195298 0,04997297 succinate dehydrogenase [ubiquinone] flavoprotein subunit, SDHA mitochondrial precursor 0,002725666 0,003153249 1,15687282 -1,966666969 0,04922162 1700026L06Rik uncharacterized protein C9orf9 homolog 0,001100807 0,001810333 1,644550394 -1,987549773 0,04686151 Gm3448 t-complex-associated testis expressed 3-like 2,21E-04 5,18E-04 2,346390172 -2,031828079 0,04217106 Valor de abundancia APEX Cociente Especie donde la proteína Proteína Nombre de la proteína Mus spretus Mus spicilegus APEX Z-score p presenta mayor abundancia PEBP1 phosphatidylethanolamine-binding protein 1 0,004091267 0,005565085 1,360235242 -2,059282999 0,03946714 Gnpda2 glucosamine-6-phosphate isomerase 2 2,57E-04 5,27E-04 2,051978809 -2,074466131 0,03803604 vdac2 voltage-dependent anion-selective channel protein 2 0,00134069 0,001860444 1,38767631 -2,115666456 0,03437319 Rab14 ras-related protein Rab-14 0,002781134 0,003648892 1,312016042 -2,12370075 0,03369517 Uchl1 ubiquitin carboxyl-terminal hydrolase isozyme L1 1,02E-04 3,75E-04 3,676995674 -2,138037871 0,03251367 YWHAB 14-3-3 protein beta/alpha 8,25E-05 3,14E-04 3,803567683 -2,138037871 0,03251367 ATP8B3 probable phospholipid-transporting ATPase IK 3,16E-04 4,58E-04 1,44682208 -2,173665011 0,0297303 VDAC1 voltage-dependent anion-selective channel protein 1 4,02E-04 7,06E-04 1,75693322 -2,179835333 0,02926967 MYCBP C-Myc-binding protein 6,69E-04 0,001381946 2,065882596 -2,180141555 0,02924697 RAB4B ras-related protein Rab-4B 4,85E-04 8,32E-04 1,715422627 -2,207099132 0,02730713 LOC231914 glucosamine-6-phosphate isomerase 1 1,24E-04 3,50E-04 2,815836993 -2,213147078 0,02688751 Gbas protein NipSnap homolog 2 1,38E-04 4,41E-04 3,194134698 -2,217894657 0,02656202 ALDH2 aldehyde dehydrogenase, mitochondrial precursor 1,49E-04 3,36E-04 2,258459618 -2,226510535 0,02598 1700029J07Rik UPF0602 protein C4orf47 homolog 2,61E-04 5,59E-04 2,14175746 -2,226510535 0,02598 NLN neurolysin, mitochondrial precursor 8,93E-05 2,17E-04 2,431274582 -2,238138299 0,02521204 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit NDUFB9 9 0,001179033 0,00187838 1,593153343 -2,275556574 0,02287256 ACE angiotensin-converting enzyme isoform 1 precursor 7,02E-04 9,41E-04 1,341219759 -2,290836687 0,02197286 GRPEL1 grpE protein homolog 1, mitochondrial precursor 1,99E-04 5,39E-04 2,713768909 -2,307576064 0,02102273 RAB2A ras-related protein Rab-2A 0,008857603 0,010802675 1,219593519 -2,310574489 0,02085637 putative ATP-dependent Clp protease proteolytic subunit, clpP mitochondrial precursor 7,00E-04 0,001231198 1,759288909 -2,319563795 0,02036449 Hspa2 heat shock-related 70 kDa protein 2 8,39E-04 0,001203049 1,433083921 -2,327842884 0,01992045

Heatr7b2 maestro heat-like repeat-containing protein family member 2B 4,86E-04 6,36E-04 1,308775409 -2,34055953 0,01925487 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase PLCZ1 zeta-1 4,64E-05 1,85E-04 3,991009802 -2,342294993 0,01916556 NDUFAF2 mimitin, mitochondrial 8,80E-05 3,57E-04 4,062239769 -2,342294993 0,01916556 Hspa4l heat shock 70 kDa protein 4L 5,07E-04 7,19E-04 1,416922015 -2,366305468 0,01796661 Gm5562 glutathione S-transferase Mu 1 1,48E-04 4,14E-04 2,788589103 -2,387256779 0,01697463 4932425I24Rik protein MAATS1 5,87E-05 1,72E-04 2,926866996 -2,387256779 0,01697463 LOC630540 WD repeat domain 63 3,10E-04 5,42E-04 1,751175127 -2,393706795 0,01667908 RAB28 ras-related protein Rab-28 9,93E-05 3,65E-04 3,68183211 -2,407617137 0,01605701 Dynlt1b dynein light chain Tctex-type 1F isoform 1 3,42E-04 0,001162115 3,396468004 -2,407617137 0,01605701

PGRMC1 membrane-associated progesterone receptor component 1 1,36E-04 4,53E-04 3,331141303 -2,407617137 0,01605701 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial ech1 precursor 8,14E-05 2,79E-04 3,421209763 -2,407617137 0,01605701 ATP1A4 sodium/potassium-transporting ATPase subunit alpha-4 0,002111607 0,002639071 1,249792612 -2,417420315 0,01563096 SLC4A1 band 3 anion transport protein 2,01E-04 4,80E-04 2,387660754 -2,420542424 0,01549737 Wdr52 WD repeat-containing protein 52 2,56E-04 3,95E-04 1,543950621 -2,483003225 0,01302799 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex NDUFA12 subunit 12 8,33E-04 0,001576319 1,892848178 -2,485326288 0,01294327 Nup53 nucleoporin NUP53 isoform 2 3,07E-04 7,74E-04 2,522307326 -2,488591441 0,01282503 EFR3A protein EFR3 homolog A 1,01E-04 2,45E-04 2,435982384 -2,518747861 0,0117773 LOC641192 heat shock cognate 71 kDa protein 3,52E-04 6,27E-04 1,783769572 -2,524685913 0,01158017

SLC2A5 solute carrier family 2, facilitated glucose transporter member 5 0,001433678 0,002375321 1,656802374 -2,571627282 0,01012218 Spna1 spectrin alpha chain, erythrocytic 1 1,07E-05 4,12E-05 3,858588954 -2,58851784 0,009639 LOC100039128 nuclear pore complex protein Nup93 2,05E-04 3,71E-04 1,805610542 -2,604765366 0,00919372 VPS13A vacuolar protein sorting-associated protein 13A 1,48E-04 2,41E-04 1,627589476 -2,613116138 0,00897208 stom erythrocyte band 7 integral membrane protein 0,00137285 0,002095574 1,526440778 -2,668607979 0,00761663 slc25a10 mitochondrial dicarboxylate carrier 9,06E-04 0,001473962 1,627432007 -2,794406861 0,0051995 Prss39 inactive serine protease 39 precursor 0,001018452 0,001858602 1,824927777 -2,794406861 0,0051995 DPEP3 dipeptidase 3 precursor 0,001421819 0,002020743 1,421237858 -2,805549411 0,00502309 4930435E12Rik uncharacterized protein LOC74663 2,29E-04 6,45E-04 2,810786446 -2,857354167 0,00427189 Pdia4 protein disulfide-isomerase A4 precursor 6,14E-05 2,10E-04 3,416557049 -2,872645309 0,00407051 RTN4IP1 reticulon-4-interacting protein 1, mitochondrial precursor 3,27E-05 2,17E-04 6,622021245 -2,877624168 0,00400682 NUP210L nuclear pore membrane glycoprotein 210-like precursor 8,65E-05 1,96E-04 2,262828362 -2,926548055 0,00342747 4930578N16Rik coiled-coil domain-containing protein 173 4,12E-05 1,75E-04 4,252399752 -2,92763113 0,00341555 CSNK2B casein kinase II subunit beta 7,91E-04 0,001834047 2,319650772 -2,964765666 0,00302914 Dnahc7a dynein, axonemal, heavy chain 7A 1,44E-05 4,57E-05 3,167551 -2,99745722 0,00272242 NIPSNAP3B protein NipSnap homolog 3B 1,31E-04 6,19E-04 4,742633439 -3,087365571 0,00201939 PPEF1 serine/threonine-protein phosphatase with EF-hands 1 2,56E-04 5,48E-04 2,140720637 -3,113057821 0,0018516 LOC100048867 annexin A2 8,23E-05 3,24E-04 3,941082603 -3,125344456 0,00177597 Ogdh 2-oxoglutarate dehydrogenase, mitochondrial isoform 1 0,001669461 0,002125695 1,273282605 -3,136519396 0,00170966 ropn1l ropporin-1-like protein 3,88E-04 9,82E-04 2,53418811 -3,14436217 0,00166449 Man2b1 lysosomal alpha-mannosidase precursor 1,99E-05 1,54E-04 7,760709224 -3,219413531 0,00128453 LDHAL6B L-lactate dehydrogenase A-like 6B 0,005257715 0,006514982 1,239128047 -3,44585512 5,69E-04

SPACA1 sperm acrosome membrane-associated protein 1 precursor 0,011496927 0,015343843 1,334603848 -3,446434926 5,68E-04 Gm5604 keratin, type II cytoskeletal 8 4,03E-05 2,66E-04 6,588921909 -3,524474817 4,24E-04 TUBB2C tubulin beta-4B chain 0,003332613 0,004455719 1,337004642 -3,649835893 2,62E-04 HEATR7A HEAT repeat containing 7A isoform 1 4,23E-05 1,60E-04 3,778266999 -3,661035915 2,51E-04 Gm16486 PREDICTED: uncharacterized protein KIAA1683 1,05E-04 3,08E-04 2,941448938 -3,728796009 1,92E-04 iars2 isoleucine--tRNA ligase, mitochondrial precursor 4,81E-04 8,76E-04 1,820470617 -3,849200882 1,19E-04 Tabla suplementaria 15. Lista de proteínas identificadas en geles 2D.

Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas ACTB actin cytoplasmic 1 641 -2,19 20 516 20 19 11 11 36 5,29 42052 ACTB actin cytoplasmic 1 676 2 14 682 31 29 12 12 41,6 5,29 42052 ACTB actin cytoplasmic 1 998 -2,04 27 314 11 9 8 7 26,1 5,29 42052 ACTB actin cytoplasmic 1 1197 -1,44 13 267 9 7 8 7 25,1 5,29 42052 ACTB actin cytoplasmic 1 1656 -4,33 8 557 22 19 12 10 43,7 5,29 42052 ACTB actin cytoplasmic 1 1881 -23,73 5 1392 63 59 24 23 70,7 5,29 42052

ALDOA fructose-bisphosphate aldolase A isoform 2 1029 -2 3 997 37 32 19 17 65 8,31 39787

ALDOA fructose-bisphosphate aldolase A isoform 2 1033 -1,55 2 1000 53 46 18 16 60,7 8,31 39787

ALDOA fructose-bisphosphate aldolase A isoform 2 1043 2 1 1115 48 41 18 16 70,3 8,31 39787

ALDOA fructose-bisphosphate aldolase A isoform 2 1047 -2 1 1125 57 52 19 18 75 8,31 39787 Prdx6 peroxiredoxin-6 471 3,74 10 283 10 10 5 5 28,1 5,98 24925 Prdx6 peroxiredoxin-6 1599 -2,41 1 949 47 44 14 13 71 5,98 24925 cct5 T-complex protein 1 subunit epsilon 558 2 4 921 32 29 17 15 42,1 5,72 60042 Cfl2 cofilin-2 1898 -5,4 13 528 18 16 10 9 53,6 7,66 18812 Gm5508 40S ribosomal protein S15 1881 -23,73 225 82 3 3 2 2 28,3 10,39 17029 CRISP2 cysteine-rich secretory protein 2 precursor 1497 4,04 22 296 9 9 5 5 26,7 6,58 28499 Tuba3a tubulin alpha-3 chain 1096 -2 18 453 18 14 10 9 25,1 4,97 50612 Tuba3a tubulin alpha-3 chain 1113 -2,48 10 451 15 13 8 7 26,7 4,97 50612 Tuba3a tubulin alpha-3 chain 1499 -2,78 15 442 16 16 9 9 28,4 4,97 50612 Tuba3a tubulin alpha-3 chain 1662 2 9 530 15 15 8 8 27,8 4,97 50612 TUBA4A tubulin alpha-4A chain 676 2 21 517 16 14 9 7 30,4 4,95 50634 TUBA4A tubulin alpha-4A chain 1881 -23,73 29 690 24 24 11 11 34 4,95 50634 Gm6682 tubulin alpha-1C chain 676 2 20 545 16 14 10 8 32 4,96 50562 Gm6682 tubulin alpha-1C chain 1881 -23,73 26 742 26 26 11 11 35,6 4,96 50562 pyruvate dehydrogenase E1 component subunit alpha testis-specific form mitochondrial pdhA2 precursor 1033 -1,55 14 472 14 11 8 8 29,4 8 44183 pyruvate dehydrogenase E1 component subunit alpha testis-specific form mitochondrial pdhA2 precursor 1043 2 14 506 17 17 9 9 27,6 8 44183 pyruvate dehydrogenase E1 component subunit alpha testis-specific form mitochondrial pdhA2 precursor 1047 -2 9 529 19 16 8 8 29,4 8 44183 serine/threonine-protein phosphatase 2B PPP3CC catalytic subunit gamma isoform 699 2,34 20 595 22 20 12 11 26,3 6,84 59403 glyceraldehyde-3-phosphate dehydrogenase GAPDHS testis-specific 555 -2,11 6 569 20 15 9 8 29,7 8,17 48096 glyceraldehyde-3-phosphate dehydrogenase GAPDHS testis-specific 568 -3,47 8 752 27 25 10 10 32 8,17 48096 glyceraldehyde-3-phosphate dehydrogenase GAPDHS testis-specific 576 1,65 7 856 46 44 12 12 47 8,17 48096 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas glyceraldehyde-3-phosphate dehydrogenase GAPDHS testis-specific 593 2,02 3 1114 74 72 16 16 63 8,17 48096 glyceraldehyde-3-phosphate dehydrogenase GAPDHS testis-specific 630 -1,78 5 544 17 15 8 7 26,7 8,17 48096 glyceraldehyde-3-phosphate dehydrogenase GAPDHS testis-specific 641 -2,19 10 705 27 26 10 10 36 8,17 48096 glyceraldehyde-3-phosphate dehydrogenase GAPDHS testis-specific 667 -2 19 603 31 30 9 9 29,2 8,17 48096 glyceraldehyde-3-phosphate dehydrogenase GAPDHS testis-specific 699 2,34 10 832 42 40 12 12 42,2 8,17 48096 glyceraldehyde-3-phosphate dehydrogenase GAPDHS testis-specific 715 2,19 12 658 28 27 9 9 29,2 8,17 48096 glyceraldehyde-3-phosphate GAPDHS dehydrogenase testis-specific 762 -2 5 1073 72 69 14 14 55,5 8,17 48096 glutamate dehydrogenase 1 mitochondrial glud1 precursor 762 -2 3 1205 59 56 22 22 43 8 61640 Krt16 keratin type I cytoskeletal 16 676 2 12 854 77 69 15 14 25,4 5,13 51973 ATP synthase subunit alpha mitochondrial Atp5a1 precursor 699 2,34 12 779 19 16 14 12 29,3 9 59830 ATP synthase subunit alpha mitochondrial Atp5a1 precursor 715 2,19 4 1126 33 29 17 15 37 9 59830 ATP synthase subunit alpha mitochondrial Atp5a1 precursor 762 -2 1 1280 49 46 18 18 38,5 9 59830 ATP synthase subunit alpha mitochondrial Atp5a1 precursor 1881 -23,73 23 822 20 17 15 12 35,1 9 59830 BPGM bisphosphoglycerate mutase 1497 4,04 15 428 16 16 8 8 53,3 6,57 30131 BPGM bisphosphoglycerate mutase 1499 -2,78 7 718 43 39 14 14 76,8 6,57 30131 BPGM bisphosphoglycerate mutase 1501 -12,23 8 585 31 30 11 11 63,7 6,57 30131 aldehyde dehydrogenase mitochondrial ALDH2 precursor 1881 -23,73 43 542 17 15 11 10 25,2 7,53 57015 cct4 T-complex protein 1 subunit delta 667 -2 3 1318 49 43 22 21 50,8 8,24 58543 cct4 T-complex protein 1 subunit delta 699 2,34 5 1235 40 37 20 19 49,4 8,24 58543 cct6a T-complex protein 1 subunit zeta 555 -2,11 5 709 27 25 13 13 36 6,63 58424 cct6a T-complex protein 1 subunit zeta 568 -3,47 4 1174 48 46 22 21 49 6,63 58424 cct6a T-complex protein 1 subunit zeta 576 1,65 3 1551 69 64 26 24 61,4 6,63 58424 cct6a T-complex protein 1 subunit zeta 594 1,87 4 883 37 32 16 15 42,6 6,63 58424 GSTM5 glutathione S-transferase Mu 5 1499 -2,78 8 712 39 38 13 13 62,1 6,82 27016 GSTM5 glutathione S-transferase Mu 5 1501 -12,23 17 367 11 9 7 5 34 6,82 27016 GSTM5 glutathione S-transferase Mu 5 1599 -2,41 5 639 30 28 11 11 53,1 6,82 27016 GSTM5 glutathione S-transferase Mu 5 1660 -2,87 21 302 11 10 6 5 28,1 6,82 27016 Gstz1 maleylacetoacetate isomerase isoform 1 1656 -4,33 16 356 15 13 8 7 49,5 7,68 24431 heterogeneous nuclear ribonucleoprotein A1 HNRNPA1L2 isoform a 1881 -23,73 78 295 5 3 5 3 29,1 9,27 34289 HSP90AA1 heat shock protein HSP 90-alpha 1881 -23,73 27 715 20 18 13 12 26,2 4,93 85134 NIT1 nitrilase homolog 1 isoform 1 1497 4,04 3 673 28 22 14 12 55,1 8 36423 NIT1 nitrilase homolog 1 isoform 1 1499 -2,78 6 791 43 39 16 15 63,2 8 36423 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas NIT1 nitrilase homolog 1 isoform 1 1501 -12,23 3 714 30 24 14 11 58,5 8 36423 Gm7204 peroxiredoxin-1 1881 -23,73 132 153 4 4 4 4 25,6 8,26 22390 Psma4 proteasome subunit alpha type-4 1492 2,19 6 534 15 14 8 7 39 7,59 29737 Psma4 proteasome subunit alpha type-4 1497 4,04 4 668 27 26 10 10 55,6 7,59 29737 Psma4 proteasome subunit alpha type-4 1501 -12,23 18 361 11 9 6 5 27,2 7,59 29737 PSMB3 proteasome subunit beta type-3 1660 -2,87 28 246 7 6 4 4 27,8 6,15 23235 PSMB3 proteasome subunit beta type-3 1662 2 31 170 5 4 4 3 27,8 6,15 23235 PYGM glycogen phosphorylase muscle form 998 -2,04 2 1448 73 69 26 25 33 6,65 97681 rps3 40S ribosomal protein S3 1881 -23,73 71 337 9 6 8 5 41,6 9,68 26828 LOC674215 40S ribosomal protein S7 1881 -23,73 96 228 7 7 5 5 40 10,09 22113 YWHAZ 14-3-3 protein zeta/delta isoform 1 676 2 31 426 14 13 6 6 33,1 4 27925 YWHAZ 14-3-3 protein zeta/delta isoform 1 1881 -23,73 44 535 21 21 8 8 33,5 4 27925 LOC100048867 annexin A2 676 2 26 475 15 12 9 8 32,4 7,55 38937 LOC100048867 annexin A2 1043 2 10 622 19 19 11 11 28 7,55 38937 LOC100048867 annexin A2 1881 -23,73 35 633 20 17 12 11 36 7,55 38937 H2afx histone H2A.x 641 -2,19 54 132 5 5 2 2 26,6 10,74 15133 H2afx histone H2A.x 676 2 59 146 7 6 3 3 31,5 10,74 15133 H2afx histone H2A.x 1492 2,19 34 122 3 3 2 2 26,6 10,74 15133 H2afx histone H2A.x 1497 4,04 37 114 3 3 2 2 26,6 10,74 15133 H2afx histone H2A.x 1499 -2,78 44 102 2 2 2 2 26,6 10,74 15133 H2afx histone H2A.x 1501 -12,23 56 76 2 2 2 2 26,6 10,74 15133 H2afx histone H2A.x 1656 -4,33 51 127 6 6 2 2 26,6 10,74 15133 H2afx histone H2A.x 1660 -2,87 38 148 4 4 2 2 26,6 10,74 15133 H2afx histone H2A.x 1662 2 35 146 5 5 2 2 26,6 10,74 15133 H2afx histone H2A.x 1881 -23,73 58 399 18 17 7 7 55,2 10,74 15133 KRT17 keratin type I cytoskeletal 17 476 5,43 5 828 28 23 15 13 30,7 5 48417 KRT17 keratin type I cytoskeletal 17 558 2 8 762 32 28 15 15 28,6 5 48417 KRT17 keratin type I cytoskeletal 17 593 2,02 6 886 33 29 17 14 30,3 5 48417 KRT17 keratin type I cytoskeletal 17 594 1,87 7 678 30 24 15 12 28,6 5 48417 KRT17 keratin type I cytoskeletal 17 676 2 7 1179 62 57 22 21 34,9 5 48417 KRT17 keratin type I cytoskeletal 17 835 -4 6 970 41 36 19 18 37,4 5 48417 KRT17 keratin type I cytoskeletal 17 1043 2 2 1099 68 57 22 20 42 5 48417 KRT17 keratin type I cytoskeletal 17 1158 -18,3 7 884 32 26 18 16 32,1 5 48417 KRT17 keratin type I cytoskeletal 17 1424 3,17 5 520 20 16 11 9 29,1 5 48417 KRT17 keratin type I cytoskeletal 17 1492 2,19 3 714 28 25 15 14 31,2 5 48417 KRT17 keratin type I cytoskeletal 17 1499 -2,78 1 1036 40 33 21 19 40 5 48417 KRT17 keratin type I cytoskeletal 17 1501 -12,23 9 574 18 16 12 11 27,5 5 48417 KRT17 keratin type I cytoskeletal 17 1881 -23,73 8 1205 45 39 23 22 38,6 5 48417 KRT17 keratin type I cytoskeletal 17 1898 -5,4 3 975 47 37 20 16 37 5 48417 PSMA7 proteasome subunit alpha type-7 1530 -2,12 3 538 15 14 10 10 52 8,59 28009 Tubb5 tubulin beta-5 chain 1881 -23,73 15 919 29 25 15 14 41,2 4,78 50095 cAMP-dependent protein kinase catalytic PRKACA subunit alpha 1033 -1,55 7 600 19 17 11 10 34 8,84 40659 cAMP-dependent protein kinase catalytic PRKACA subunit alpha 1096 -2 4 742 27 19 15 12 39 8,84 40659 Ldhc L-lactate dehydrogenase C chain 473 -3,03 12 377 11 10 7 7 26,8 8,43 36231 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas Ldhc L-lactate dehydrogenase C chain 1033 -1,55 22 317 9 8 7 6 28,3 8,43 36231 Ldhc L-lactate dehydrogenase C chain 1047 -2 8 530 14 14 10 10 40,4 8,43 36231 Ldhc L-lactate dehydrogenase C chain 1096 -2 11 507 18 15 9 9 35,5 8,43 36231 Ldhc L-lactate dehydrogenase C chain 1197 -1,44 10 324 10 9 7 6 25,6 8,43 36231 Odf2 outer dense fiber protein 2 isoform c 471 3,74 4 771 27 25 15 14 27,9 7,21 74084 Odf2 outer dense fiber protein 2 isoform c 558 2 3 1128 39 35 19 15 37 7,21 74084 Odf2 outer dense fiber protein 2 isoform c 998 -2,04 1 1685 94 81 27 22 38,4 7,21 74084 Odf2 outer dense fiber protein 2 isoform c 1140 4,63 2 921 31 27 16 15 25,7 7,21 74084 ZPBP zona pellucida-binding protein 1 isoform 1 1113 -2,48 7 501 18 16 10 9 30,4 9 45850 H2AFZ histone H2A.Z 1881 -23,73 114 179 8 8 3 3 35 10,58 13545 serine/threonine-protein phosphatase 2A PPP2CB catalytic subunit beta isoform 1197 -1,44 2 588 19 19 9 9 39,5 5,21 36123 PGAM2 phosphoglycerate mutase 2 1497 4,04 5 653 40 35 11 11 49 8,65 28980 PGAM2 phosphoglycerate mutase 2 1499 -2,78 12 489 19 17 9 9 44,3 8,65 28980 PGAM2 phosphoglycerate mutase 2 1501 -12,23 11 533 24 22 11 9 52,2 8,65 28980 PGAM2 phosphoglycerate mutase 2 1529 -3,69 1 1134 117 95 19 19 76,3 8,65 28980 PGAM2 phosphoglycerate mutase 2 1530 -2,12 1 1091 78 74 20 18 76,3 8,65 28980 Fbp1 fructose-1 6-bisphosphatase 1 1096 -2 2 833 33 31 13 12 53,3 6,15 37288 Fbp1 fructose-1 6-bisphosphatase 1 1140 4,63 1 1469 82 76 21 19 82 6,15 37288 Fbp1 fructose-1 6-bisphosphatase 1 1158 -18,3 2 1474 80 78 20 20 69,5 6,15 37288 Fbp1 fructose-1 6-bisphosphatase 1 1434 4,17 10 356 13 12 7 6 27,2 6,15 37288 pfkp 6-phosphofructokinase type C 348 2,33 1 2319 170 160 31 31 54,3 6,73 86427 Gm5928 ubiquitin-60S ribosomal protein L40 507 -1,61 31 203 9 7 4 3 36 9 15004 Gm5928 ubiquitin-60S ribosomal protein L40 630 -1,78 20 261 11 8 5 4 41,4 9 15004 Gm5928 ubiquitin-60S ribosomal protein L40 641 -2,19 27 350 19 17 7 7 43 9 15004 Gm5928 ubiquitin-60S ribosomal protein L40 1881 -23,73 81 285 9 9 6 6 43 9 15004 succinyl-CoA:3-ketoacid-coenzyme A Oxct2a transferase 2A mitochondrial precursor 667 -2 4 1231 45 41 20 20 58,7 9,07 56950 succinyl-CoA:3-ketoacid-coenzyme A Oxct2a transferase 2A mitochondrial precursor 676 2 9 1162 47 44 18 17 53,1 9,07 56950 succinyl-CoA:3-ketoacid-coenzyme A Oxct2a transferase 2A mitochondrial precursor 699 2,34 4 1411 71 64 20 20 60,4 9,07 56950 succinyl-CoA:3-ketoacid-coenzyme A Oxct2a transferase 2A mitochondrial precursor 715 2,19 8 1045 46 41 16 15 46,3 9,07 56950 succinyl-CoA:3-ketoacid-coenzyme A Oxct2a transferase 2A mitochondrial precursor 1029 -2 8 544 17 16 9 9 29,8 9,07 56950 succinyl-CoA:3-ketoacid-coenzyme A Oxct2a transferase 2A mitochondrial precursor 1043 2 7 730 24 22 11 10 35 9,07 56950 succinyl-CoA:3-ketoacid-coenzyme A Oxct2a transferase 2A mitochondrial precursor 1047 -2 2 881 36 34 13 12 39,6 9,07 56950 succinyl-CoA:3-ketoacid-coenzyme A Oxct2a transferase 2A mitochondrial precursor 1096 -2 14 489 15 13 8 6 27,5 9,07 56950 succinyl-CoA:3-ketoacid-coenzyme A Oxct2a transferase 2A mitochondrial precursor 1158 -18,3 10 576 20 19 10 10 31,2 9,07 56950 succinyl-CoA:3-ketoacid-coenzyme A Oxct2a transferase 2A mitochondrial precursor 1501 -12,23 4 679 19 16 11 9 33 9,07 56950 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10 mitochondrial NDUFA10 precursor 1104 -2 1 951 41 38 17 17 56,9 7,63 40863 ACY1 aminoacylase-1 998 -2,04 6 640 17 13 11 9 35 5,81 45994 cyc1 cytochrome c1 heme protein mitochondrial 857 -2,65 25 304 8 7 6 6 29,5 9,24 35533 cyc1 cytochrome c1 heme protein mitochondrial 1423 2 3 691 38 35 12 12 60 9,24 35533 cyc1 cytochrome c1 heme protein mitochondrial 1424 3,17 1 798 49 45 13 12 62,2 9,24 35533 cyc1 cytochrome c1 heme protein mitochondrial 1434 4,17 13 320 9 9 6 6 29,5 9,24 35533 26S proteasome non-ATPase regulatory PSMD12 subunit 12 667 -2 13 759 22 19 13 12 39 6,66 53261 26S proteasome non-ATPase regulatory PSMD12 subunit 12 715 2,19 1 1347 47 44 20 19 53,7 6,66 53261 26S proteasome non-ATPase regulatory PSMD12 subunit 12 762 -2 4 1092 37 37 17 17 49,1 6,66 53261 Actc1 actin alpha cardiac muscle 1 1881 -23,73 18 889 37 33 17 16 40 5,23 42334 Hbb-b2 hemoglobin subunit beta-2 1529 -3,69 32 204 6 6 4 4 32 7,85 15982 aconitate hydratase mitochondrial ACO2 precursor 345 -4,47 1 2089 123 111 36 34 57,7 8,08 86151

ACO2 aconitate hydratase mitochondrial precursor 348 2,33 2 1755 88 80 28 27 45,5 8,08 86151 aconitate hydratase mitochondrial ACO2 precursor 353 -3,1 1 2449 136 127 40 37 64,2 8,08 86151

ACO2 aconitate hydratase mitochondrial precursor 473 -3,03 1 1421 54 53 24 23 42,6 8,08 86151

ACO2 aconitate hydratase mitochondrial precursor 476 5,43 3 897 30 30 17 17 27,4 8,08 86151

ACO2 aconitate hydratase mitochondrial precursor 478 -5,45 2 1037 32 31 20 20 32,1 8,08 86151

ACO2 aconitate hydratase mitochondrial precursor 699 2,34 6 1134 34 32 19 19 29,5 8,08 86151

pyruvate dehydrogenase E1 component Gm6123 subunit beta mitochondrial precursor 1197 -1,44 1 1055 105 99 15 15 60,7 6,41 39254 pyruvate dehydrogenase E1 component subunit Gm6123 beta mitochondrial precursor 1434 4,17 2 753 38 37 13 13 52,1 6,41 39254 pyruvate dehydrogenase E1 component subunit Gm6123 beta mitochondrial precursor 1599 -2,41 10 430 11 9 8 7 30,1 6,41 39254 isocitrate dehydrogenase [NAD] subunit idh3a alpha mitochondrial precursor 1140 4,63 11 506 16 15 10 9 34 6,27 40069 succinyl-CoA:3-ketoacid-coenzyme A oxct1 transferase 1 mitochondrial precursor 715 2,19 6 1096 47 45 16 15 48,3 8,73 56352 IDH3B isocitrate dehydrogenase 3 beta subunit 1047 -2 6 628 27 24 12 11 35,9 8,76 42453 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas IDH3B isocitrate dehydrogenase 3 beta subunit 1096 -2 5 610 30 26 13 13 38 8,76 42453

methylmalonate-semialdehyde dehydrogenase Aldh6a1 [acylating] mitochondrial precursor 762 -2 2 1245 45 43 20 19 43 8 58335 HIST2H3B histone H2A type 2-A 641 -2,19 49 160 5 5 2 2 29,2 10,9 14087 HIST2H3B histone H2A type 2-A 676 2 57 146 6 5 3 3 34,6 10,9 14087 HIST2H3B histone H2A type 2-A 1497 4,04 42 96 2 2 2 2 29,2 10,9 14087 HIST2H3B histone H2A type 2-A 1501 -12,23 47 98 2 2 2 2 29,2 10,9 14087 HIST2H3B histone H2A type 2-A 1656 -4,33 43 152 8 6 3 2 43,1 10,9 14087 HIST2H3B histone H2A type 2-A 1660 -2,87 42 132 3 3 2 2 29,2 10,9 14087 HIST2H3B histone H2A type 2-A 1662 2 34 155 5 4 3 2 43,1 10,9 14087 HIST2H3B histone H2A type 2-A 1881 -23,73 52 426 27 23 7 7 57,7 10,9 14087 KRT5 keratin type II cytoskeletal 5 558 2 2 1129 68 58 24 19 27,6 7,59 61957 KRT5 keratin type II cytoskeletal 5 568 -3,47 6 968 50 43 19 17 29,8 7,59 61957 KRT5 keratin type II cytoskeletal 5 576 1,65 6 874 49 40 18 15 29,7 7,59 61957 KRT5 keratin type II cytoskeletal 5 593 2,02 5 990 43 40 20 20 29,8 7,59 61957 KRT5 keratin type II cytoskeletal 5 594 1,87 2 982 70 64 22 20 28,1 7,59 61957 KRT5 keratin type II cytoskeletal 5 667 -2 7 1033 48 41 23 20 32 7,59 61957 KRT5 keratin type II cytoskeletal 5 676 2 4 1445 96 87 29 25 33,6 7,59 61957 KRT5 keratin type II cytoskeletal 5 835 -4 2 1173 72 66 24 21 27,4 7,59 61957 KRT5 keratin type II cytoskeletal 5 857 -2,65 7 668 30 23 16 12 26,2 7,59 61957 KRT5 keratin type II cytoskeletal 5 998 -2,04 4 991 46 40 19 18 26,4 7,59 61957 KRT5 keratin type II cytoskeletal 5 1043 2 3 1043 70 59 24 20 25,3 7,59 61957 KRT5 keratin type II cytoskeletal 5 1158 -18,3 3 1214 68 64 25 24 27,9 7,59 61957 KRT5 keratin type II cytoskeletal 5 1288 -12,4 2 903 34 27 19 17 25,2 7,59 61957 KRT5 keratin type II cytoskeletal 5 1492 2,19 1 983 52 50 19 18 31,2 7,59 61957 KRT5 keratin type II cytoskeletal 5 1499 -2,78 2 918 44 40 19 18 25,7 7,59 61957 KRT5 keratin type II cytoskeletal 5 1529 -3,69 2 1059 37 36 22 22 28,6 7,59 61957 KRT5 keratin type II cytoskeletal 5 1656 -4,33 1 1102 53 44 21 18 27,8 7,59 61957 KRT5 keratin type II cytoskeletal 5 1660 -2,87 1 887 36 29 20 17 26 7,59 61957 KRT5 keratin type II cytoskeletal 5 1662 2 1 1049 40 37 20 18 27,4 7,59 61957 KRT5 keratin type II cytoskeletal 5 1881 -23,73 3 1526 99 93 30 27 35 7,59 61957 KRT5 keratin type II cytoskeletal 5 1898 -5,4 1 1114 67 62 22 21 27,6 7,59 61957 serine/threonine-protein phosphatase 6 PPP6C catalytic subunit 1197 -1,44 5 479 17 17 10 10 42,6 5,43 35821 LRP2BP LRP2-binding protein 1096 -2 10 511 14 13 9 8 36,4 6,84 40120

coiled-coil-helix-coiled-coil-helix domain- CHCHD3 containing protein 3 mitochondrial precursor 1530 -2,12 6 414 12 11 7 6 30,4 8,56 26546

UGP2 UTP--glucose-1-phosphate uridylyltransferase 762 -2 7 794 34 33 15 14 35,6 7,18 57115 Hist1h4c histone H4 641 -2,19 45 197 6 6 4 4 38 11,36 11360 Hist1h4c histone H4 676 2 77 87 3 2 3 2 29,1 11,36 11360 Hist1h4c histone H4 1656 -4,33 48 134 4 4 3 3 38 11,36 11360 Hist1h4c histone H4 1881 -23,73 60 389 17 16 7 6 55,3 11,36 11360 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas acetyl-CoA acetyltransferase mitochondrial ACAT1 precursor 1033 -1,55 9 562 23 22 11 11 36 8 45129 EIF4A1 eukaryotic initiation factor 4A-I isoform 1 1881 -23,73 28 704 17 15 12 12 42,4 5,32 46353 Krt14 keratin type I cytoskeletal 14 348 2,33 5 638 30 28 12 11 26,4 5 53176 Krt14 keratin type I cytoskeletal 14 353 -3,1 3 633 25 23 13 13 27,9 5 53176 Krt14 keratin type I cytoskeletal 14 476 5,43 7 816 38 32 15 14 29,5 5 53176 Krt14 keratin type I cytoskeletal 14 558 2 6 823 44 38 14 14 26 5 53176 Krt14 keratin type I cytoskeletal 14 568 -3,47 7 862 37 30 17 13 31,2 5 53176 Krt14 keratin type I cytoskeletal 14 593 2,02 8 832 39 30 16 11 27,3 5 53176 Krt14 keratin type I cytoskeletal 14 594 1,87 5 824 41 34 17 12 31 5 53176 Krt14 keratin type I cytoskeletal 14 676 2 2 1529 123 108 27 25 39,9 5 53176 Krt14 keratin type I cytoskeletal 14 835 -4 5 1007 62 52 20 19 34 5 53176 Krt14 keratin type I cytoskeletal 14 1043 2 4 899 52 42 17 15 30,8 5 53176 Krt14 keratin type I cytoskeletal 14 1108 -2,66 2 859 44 39 17 16 28,9 5 53176 Krt14 keratin type I cytoskeletal 14 1140 4,63 5 727 30 27 13 12 26,2 5 53176 Krt14 keratin type I cytoskeletal 14 1158 -18,3 6 898 49 38 18 15 25,6 5 53176 Krt14 keratin type I cytoskeletal 14 1423 2 7 506 21 17 10 8 25,2 5 53176 Krt14 keratin type I cytoskeletal 14 1434 4,17 4 597 20 16 11 8 27,5 5 53176 Krt14 keratin type I cytoskeletal 14 1492 2,19 2 730 32 26 16 13 29,8 5 53176 Krt14 keratin type I cytoskeletal 14 1497 4,04 2 779 34 27 15 13 25,8 5 53176 Krt14 keratin type I cytoskeletal 14 1499 -2,78 3 911 48 40 17 15 30,8 5 53176 Krt14 keratin type I cytoskeletal 14 1501 -12,23 6 626 27 25 12 11 25,6 5 53176 Krt14 keratin type I cytoskeletal 14 1656 -4,33 2 1025 59 44 19 14 34,9 5 53176 Krt14 keratin type I cytoskeletal 14 1660 -2,87 3 695 31 20 13 10 28,5 5 53176 Krt14 keratin type I cytoskeletal 14 1662 2 2 823 45 37 15 13 28,3 5 53176 Krt14 keratin type I cytoskeletal 14 1881 -23,73 4 1426 80 68 26 25 40 5 53176 Krt14 keratin type I cytoskeletal 14 1898 -5,4 2 1010 55 43 20 16 30,4 5 53176 abhydrolase domain-containing protein 11 abhd11 isoform 1 1492 2,19 8 441 19 14 9 8 33 9,64 33654 NAD-dependent malic enzyme ME2 mitochondrial precursor 555 -2,11 2 1254 49 49 20 20 54,3 7,53 66441 NAD-dependent malic enzyme ME2 mitochondrial precursor 568 -3,47 5 1011 24 22 17 15 41,8 7,53 66441

bifunctional ATP-dependent dihydroxyacetone dak kinase/FAD-AMP lyase (cyclizing) 507 -1,61 3 1009 32 27 16 13 39,4 6,44 59938 bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase dak (cyclizing) 555 -2,11 1 2199 149 138 30 28 83,2 6,44 59938

bifunctional ATP-dependent dihydroxyacetone dak kinase/FAD-AMP lyase (cyclizing) 568 -3,47 1 1773 71 70 23 23 62,1 6,44 59938

bifunctional ATP-dependent dihydroxyacetone dak kinase/FAD-AMP lyase (cyclizing) 576 1,65 4 1359 46 43 19 19 52,2 6,44 59938 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas

bifunctional ATP-dependent dihydroxyacetone dak kinase/FAD-AMP lyase (cyclizing) 593 2,02 7 837 27 25 14 13 45,7 6,44 59938

bifunctional ATP-dependent dihydroxyacetone dak kinase/FAD-AMP lyase (cyclizing) 667 -2 10 897 27 24 14 14 37,5 6,44 59938 bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase dak (cyclizing) 699 2,34 1 1847 94 91 25 25 63 6,44 59938

bifunctional ATP-dependent dihydroxyacetone dak kinase/FAD-AMP lyase (cyclizing) 715 2,19 14 604 20 20 9 9 26 6,44 59938 3-hydroxyisobutyrate dehydrogenase hibadh mitochondrial precursor 1423 2 2 770 30 29 11 11 45,4 8 35816 3-hydroxyisobutyrate dehydrogenase hibadh mitochondrial precursor 1434 4,17 1 1058 50 50 14 14 65 8 35816 sorD sorbitol dehydrogenase 476 5,43 15 522 19 18 10 10 38 6,56 38795 sorD sorbitol dehydrogenase 699 2,34 13 724 29 28 12 12 45,9 6,56 38795 sorD sorbitol dehydrogenase 715 2,19 5 1107 64 61 19 19 64 6,56 38795 sorD sorbitol dehydrogenase 857 -2,65 5 736 30 28 12 11 45,4 6,56 38795 sorD sorbitol dehydrogenase 1029 -2 5 706 31 30 13 13 47,1 6,56 38795 sorD sorbitol dehydrogenase 1033 -1,55 16 414 11 10 8 8 30,3 6,56 38795 sorD sorbitol dehydrogenase 1096 -2 1 1096 69 68 18 18 64,7 6,56 38795 sorD sorbitol dehydrogenase 1104 -2 2 930 52 45 15 15 57,7 6,56 38795 sorD sorbitol dehydrogenase 1108 -2,66 1 1221 96 90 20 20 67,8 6,56 38795 sorD sorbitol dehydrogenase 1113 -2,48 1 1226 114 106 19 19 65,8 6,56 38795 sorD sorbitol dehydrogenase 1120 2,16 1 1413 161 148 23 21 71,7 6,56 38795 sorD sorbitol dehydrogenase 1158 -18,3 21 395 14 13 7 7 26,3 6,56 38795 sorD sorbitol dehydrogenase 1288 -12,4 10 516 18 18 9 9 33 6,56 38795 sorD sorbitol dehydrogenase 1660 -2,87 19 337 12 11 8 8 30 6,56 38795 TUBB2C tubulin beta-4B chain 1881 -23,73 14 1006 36 31 16 15 48,5 4,79 50255 cAMP-dependent protein kinase type II-alpha PRKAR2A regulatory subunit 1197 -1,44 3 545 14 14 8 8 27,9 4,82 45903 phosphoethanolamine/phosphocholine PHOSPHO1 phosphatase 1497 4,04 26 238 8 6 6 4 27,7 8 30348 phosphoethanolamine/phosphocholine PHOSPHO1 phosphatase 1499 -2,78 30 231 7 6 6 5 28,1 8 30348 ccdc65 coiled-coil domain-containing protein 65 641 -2,19 6 1016 28 28 20 20 41 6,51 58305 ccdc65 coiled-coil domain-containing protein 65 667 -2 8 978 31 30 19 18 40 6,51 58305

COASY bifunctional coenzyme A synthase precursor 641 -2,19 12 617 18 18 11 11 25,2 6,61 62213 Psmc6 26S protease regulatory subunit 10B 1029 -2 1 1124 58 53 21 20 59,9 7,1 44430 Psmc6 26S protease regulatory subunit 10B 1033 -1,55 1 1095 67 64 18 18 55,3 7,1 44430 RAE1 mRNA export factor 1033 -1,55 13 497 19 16 11 9 36 7,96 41566 RAE1 mRNA export factor 1047 -2 5 636 26 25 12 11 41 7,96 41566 Hist1h2bb histone H2B type 1-B 641 -2,19 44 200 9 9 3 3 26,2 10,31 13944 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas Hist1h2bb histone H2B type 1-B 1656 -4,33 30 227 8 6 5 3 39 10,31 13944 Hist1h2bb histone H2B type 1-B 1662 2 29 186 6 4 5 3 39 10,31 13944 Hist1h2bb histone H2B type 1-B 1881 -23,73 86 275 12 11 5 4 38,1 10,31 13944 JUP junction plakoglobin 476 5,43 4 869 29 28 16 16 25,2 5,75 82490 JUP junction plakoglobin 641 -2,19 5 1108 41 40 19 19 30,5 5,75 82490 JUP junction plakoglobin 676 2 5 1372 67 62 25 23 41,6 5,75 82490 JUP junction plakoglobin 1881 -23,73 6 1367 57 55 24 23 38 5,75 82490 cyb5r2 NADH-cytochrome b5 reductase 2 isoform 2 1424 3,17 9 415 15 14 7 7 30,1 6,26 31397 Prdx6-rs1 peroxiredoxin 6 related sequence 1 1599 -2,41 7 521 19 16 10 8 58,5 5,98 25151 H2afj histone H2A.J 1881 -23,73 47 449 21 19 7 7 61,2 11,05 14037 fumarylacetoacetate hydrolase domain- Fahd2a containing protein 2A 1288 -12,4 1 1063 107 102 14 14 59,7 8,42 35053 fumarylacetoacetate hydrolase domain- Fahd2a containing protein 2A 1898 -5,4 10 547 32 30 10 9 44,1 8,42 35053 GMPR2 GMP reductase 2 1096 -2 22 382 15 14 10 9 32 7,12 38507 Hist1h2bc histone H2B type 1-C/E/G 1660 -2,87 41 138 3 3 3 3 38,1 10,31 13898 axonemal dynein light intermediate DNALI1 polypeptide 1 1423 2 5 528 17 15 10 9 44,2 8 29947 axonemal dynein light intermediate DNALI1 polypeptide 1 1424 3,17 10 399 13 10 8 5 34,5 8 29947 axonemal dynein light intermediate DNALI1 polypeptide 1 1431 -4 5 538 20 18 10 10 38,4 8 29947 axonemal dynein light intermediate DNALI1 polypeptide 1 1499 -2,78 16 408 13 9 9 6 36 8 29947 axonemal dynein light intermediate DNALI1 polypeptide 1 1529 -3,69 3 619 22 21 10 10 47,7 8 29947 TAGLN2 transgelin-2 1881 -23,73 51 430 9 8 8 7 45,7 8,39 22552 sorting and assembly machinery component 50 Samm50 homolog 715 2,19 9 885 26 25 16 15 41,8 6,34 52230 PPME1 protein phosphatase methylesterase 1 998 -2,04 5 893 30 29 15 14 47,4 5,67 42628 LOC677611 casein kinase II subunit alpha 1029 -2 4 800 25 25 14 14 44,5 7,29 45219 LOC677611 casein kinase II subunit alpha 1033 -1,55 5 643 17 13 13 11 43,2 7,29 45219 YWHAB 14-3-3 protein beta/alpha 1881 -23,73 55 420 13 13 7 7 38 4 28183 ATP synthase subunit beta mitochondrial ATP5B precursor 1599 -2,41 3 741 32 32 12 12 34 5,19 56265 ATP synthase subunit beta mitochondrial ATP5B precursor 1881 -23,73 10 1156 34 33 18 18 52,2 5,19 56265 serine/threonine-protein phosphatase PP1- LOC627816 gamma catalytic subunit 1140 4,63 7 693 27 26 13 13 47,4 6,12 37701

Gm6560 pyruvate kinase isozymes M1/M2 isoform 1 473 -3,03 3 855 27 26 14 13 37,5 7,18 58378

Gm6560 pyruvate kinase isozymes M1/M2 isoform 1 593 2,02 2 1843 84 81 27 27 63,7 7,18 58378

Gm6560 pyruvate kinase isozymes M1/M2 isoform 1 630 -1,78 1 1680 61 58 26 25 63,7 7,18 58378 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas

Gm6560 pyruvate kinase isozymes M1/M2 isoform 1 641 -2,19 1 1685 63 62 25 25 61 7,18 58378

Gm6560 pyruvate kinase isozymes M1/M2 isoform 1 667 -2 2 2115 101 95 31 30 66,7 7,18 58378 LOC641192 heat shock cognate 71 kDa protein 1881 -23,73 16 907 26 22 17 15 37 5,37 71055 enoyl-CoA delta isomerase 1 mitochondrial dci precursor 1529 -3,69 6 509 18 18 8 8 39,4 9 32515 enoyl-CoA delta isomerase 1 mitochondrial dci precursor 1530 -2,12 4 439 15 14 7 6 34 9 32515 Tubb4 tubulin beta-4A chain 1881 -23,73 20 858 30 27 14 13 44,8 4,78 50010 Hbb-b2 hemoglobin subunit beta-1 1492 2,19 25 176 5 4 3 2 25,9 7,14 15795 Hbb-b2 hemoglobin subunit beta-1 1497 4,04 23 293 6 5 6 5 56,5 7,14 15795 Hbb-b2 hemoglobin subunit beta-1 1529 -3,69 23 287 9 8 5 5 43,5 7,14 15795 GLUL glutamine synthetase 699 2,34 34 400 9 9 8 8 25,5 6,64 42834 LOC635419 alcohol dehydrogenase class-3 1096 -2 8 529 19 16 9 8 30,7 6,97 40320 dihydrolipoyl dehydrogenase mitochondrial dld precursor 699 2,34 21 580 19 14 10 8 28,5 7,99 54751 dihydrolipoyl dehydrogenase mitochondrial dld precursor 715 2,19 3 1184 64 55 19 18 56,4 7,99 54751 ly6/PLAUR domain-containing protein 4 Lypd4 precursor 1529 -3,69 15 333 13 13 5 5 31,7 7,88 27834 PSMA1 proteasome subunit alpha type-1 1423 2 20 228 8 7 6 6 31,2 6 29813 PSMA1 proteasome subunit alpha type-1 1424 3,17 2 656 35 33 13 11 52,1 6 29813 PSMA1 proteasome subunit alpha type-1 1434 4,17 24 231 11 9 6 5 26,2 6 29813 Gm8894 myosin light polypeptide 6 1881 -23,73 83 280 11 9 5 5 40,4 4,46 17121 Tubb2a tubulin beta-2A chain 1881 -23,73 22 835 27 23 15 13 43,4 4,78 50274

Ak2 adenylate kinase 2 mitochondrial isoform b 1499 -2,78 29 238 8 6 6 5 33 6,97 25817

Ak2 adenylate kinase 2 mitochondrial isoform b 1529 -3,69 12 364 16 16 7 7 37,9 6,97 25817 succinate dehydrogenase [ubiquinone] iron- LOC638710 sulfur subunit mitochondrial precursor 1530 -2,12 2 797 40 36 16 14 53,5 8 32591 Gm7839 tropomyosin alpha-3 chain isoform 1 1881 -23,73 109 188 7 2 7 2 28,9 4 33357 HSP90AB1 heat shock protein HSP 90-beta 1881 -23,73 13 1037 28 26 17 16 32,6 4,97 83571 succinyl-CoA ligase [ADP-forming] subunit SUCLA2 beta mitochondrial precursor 998 -2,04 3 1439 56 49 25 21 58,7 6,57 50424

succinate dehydrogenase [ubiquinone] SDHA flavoprotein subunit mitochondrial precursor 471 3,74 2 1318 55 51 20 19 43,2 7,06 73623

succinate dehydrogenase [ubiquinone] SDHA flavoprotein subunit mitochondrial precursor 480 20,72 1 1381 61 57 21 21 44,6 7,06 73623

succinate dehydrogenase [ubiquinone] SDHA flavoprotein subunit mitochondrial precursor 506 -1,55 1 2284 154 145 34 33 77 7,06 73623 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial SDHA precursor 507 -1,61 1 1873 110 101 29 27 63,3 7,06 73623 KRT6A keratin type II cytoskeletal 6A 676 2 3 1459 106 96 27 24 30,9 8 59641 KRT6A keratin type II cytoskeletal 6A 1158 -18,3 4 953 54 46 21 17 25,1 8 59641 1700026L06Rik uncharacterized protein C9orf9 homolog 1529 -3,69 35 187 5 5 3 3 28,6 7,16 19812 1700026L06Rik uncharacterized protein C9orf9 homolog 1656 -4,33 18 314 14 14 5 5 38 7,16 19812 1700026L06Rik uncharacterized protein C9orf9 homolog 1660 -2,87 31 211 7 5 4 3 37,5 7,16 19812 1700026L06Rik uncharacterized protein C9orf9 homolog 1662 2 25 271 8 8 5 5 38 7,16 19812 1700026L06Rik uncharacterized protein C9orf9 homolog 1881 -23,73 56 418 24 21 8 8 64,3 7,16 19812

1700026L06Rik uncharacterized protein C9orf9 homolog 1898 -5,4 15 477 40 35 9 8 72 7,16 19812 tekt3 tektin-3 699 2,34 2 1490 65 62 26 25 55,7 6,59 57094 mitochondrial inner membrane protein isoform IMMT 1 558 2 9 725 26 20 16 12 26,9 6,18 84247 Gm5506 uncharacterized protein LOC433182 835 -4 4 1013 37 34 15 14 47,5 6,37 47453 Gm5506 uncharacterized protein LOC433182 857 -2,65 6 728 28 24 13 12 38,5 6,37 47453 Crat carnitine O-acetyltransferase 593 2,02 4 1026 47 39 20 18 39,1 8 71250 phospholipid hydroperoxide glutathione hnrpll peroxidase nuclear isoform 1 1898 -5,4 23 330 12 9 8 5 32 10,25 29803 TCP1 T-complex protein 1 subunit alpha 558 2 1 1895 99 94 27 26 64,2 5,82 60867 Gstm7 glutathione S-transferase Mu 7 1660 -2,87 2 736 32 30 13 13 66 6,34 25864 Hspa1l heat shock 70 kDa protein 1B 1881 -23,73 9 1183 36 36 18 18 42,2 5,53 70418 Hspa1l heat shock 70 kDa protein 1-like 471 3,74 1 1390 55 48 23 20 51,5 5,91 70992 Hspa1l heat shock 70 kDa protein 1-like 473 -3,03 2 988 36 33 17 15 35,6 5,91 70992 Hspa1l heat shock 70 kDa protein 1-like 476 5,43 1 983 31 29 18 16 40,1 5,91 70992 Hspa1l heat shock 70 kDa protein 1-like 478 -5,45 1 1150 44 43 18 18 38,1 5,91 70992 Hspa1l heat shock 70 kDa protein 1-like 480 20,72 2 877 28 24 17 14 35,9 5,91 70992 Hspa1l heat shock 70 kDa protein 1-like 506 -1,55 2 1637 73 69 24 24 54 5,91 70992 Hspa1l heat shock 70 kDa protein 1-like 507 -1,61 2 1541 62 57 22 22 49,5 5,91 70992 6-phosphogluconate dehydrogenase PGD decarboxylating 857 -2,65 12 527 18 15 10 10 26,3 6,81 53726 hsdl2 hydroxysteroid dehydrogenase-like protein 2 715 2,19 15 580 12 11 10 9 25,1 6,31 54516 Eef1a1 elongation factor 1-alpha 1 1881 -23,73 21 843 39 36 18 15 40,5 9,1 50424 GAS8 growth arrest-specific protein 8 667 -2 12 804 36 29 20 18 38 7,68 56400 Gm5279 14-3-3 protein sigma 676 2 28 452 16 16 9 9 41,9 4,72 27803 Gm5279 14-3-3 protein sigma 1656 -4,33 32 222 10 9 5 5 35,9 4,72 27803 Gm5279 14-3-3 protein sigma 1881 -23,73 24 792 42 33 16 14 49,6 4,72 27803 psmA2 proteasome subunit alpha type-2 1529 -3,69 20 302 10 9 5 4 35,9 6,92 26024 psmA2 proteasome subunit alpha type-2 1660 -2,87 4 664 27 26 9 9 59 6,92 26024 psmA2 proteasome subunit alpha type-2 1662 2 3 753 47 45 11 10 59,4 6,92 26024 wdr16 WD repeat-containing protein 16 507 -1,61 4 888 31 28 15 15 31,8 6,36 69223 TEKT2 tektin-2 835 -4 1 1682 72 65 28 25 66,5 5,98 50736 phosphatidylethanolamine-binding protein 4 Pebp4 isoform a precursor 1662 2 15 384 13 13 7 7 30,6 5,3 27125 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas Acat2 acetyl-CoA acetyltransferase cytosolic 1096 -2 7 558 27 23 11 10 37,5 7,16 41727 Acat2 acetyl-CoA acetyltransferase cytosolic 1108 -2,66 8 562 16 16 10 10 40,1 7,16 41727 Acat2 acetyl-CoA acetyltransferase cytosolic 1120 2,16 7 408 12 12 8 8 27,5 7,16 41727 Krt42 keratin type I cytoskeletal 42 476 5,43 9 677 31 23 14 11 28,3 5,09 50444 Krt42 keratin type I cytoskeletal 42 480 20,72 7 540 18 15 11 10 25,4 5,09 50444 Krt42 keratin type I cytoskeletal 42 558 2 5 851 48 37 18 14 33,6 5,09 50444 Krt42 keratin type I cytoskeletal 42 568 -3,47 10 705 29 26 13 12 27 5,09 50444 Krt42 keratin type I cytoskeletal 42 576 1,65 10 676 26 22 14 13 27,7 5,09 50444 Krt42 keratin type I cytoskeletal 42 594 1,87 6 696 36 26 16 10 31 5,09 50444 Krt42 keratin type I cytoskeletal 42 667 -2 16 703 23 20 14 13 27 5,09 50444 Krt42 keratin type I cytoskeletal 42 676 2 6 1349 92 80 27 24 38,9 5,09 50444 Krt42 keratin type I cytoskeletal 42 699 2,34 17 616 22 18 15 11 27 5,09 50444 Krt42 keratin type I cytoskeletal 42 835 -4 7 916 50 38 18 16 32,5 5,09 50444 Krt42 keratin type I cytoskeletal 42 1043 2 5 884 63 51 19 16 38,9 5,09 50444 Krt42 keratin type I cytoskeletal 42 1108 -2,66 4 801 39 33 15 14 27 5,09 50444 Krt42 keratin type I cytoskeletal 42 1140 4,63 8 632 29 25 13 12 27,7 5,09 50444 Krt42 keratin type I cytoskeletal 42 1158 -18,3 5 917 51 40 19 16 29,2 5,09 50444 Krt42 keratin type I cytoskeletal 42 1288 -12,4 4 804 46 37 17 13 31,9 5,09 50444 Krt42 keratin type I cytoskeletal 42 1431 -4 3 631 24 22 13 13 26,8 5,09 50444 Krt42 keratin type I cytoskeletal 42 1492 2,19 5 637 27 22 14 12 27 5,09 50444 Krt42 keratin type I cytoskeletal 42 1499 -2,78 5 830 43 36 17 16 32,1 5,09 50444 Krt42 keratin type I cytoskeletal 42 1656 -4,33 3 770 35 28 16 12 27 5,09 50444 Krt42 keratin type I cytoskeletal 42 1881 -23,73 11 1086 59 50 19 18 30,1 5,09 50444 Krt42 keratin type I cytoskeletal 42 1898 -5,4 5 845 46 39 18 16 34 5,09 50444 mitochondrial import receptor subunit TOM40 tomm40 homolog 1029 -2 11 466 20 19 7 7 25,2 7,64 38270 Car2 carbonic anhydrase 2 1424 3,17 17 319 11 10 6 6 39 6,49 29129 Car2 carbonic anhydrase 2 1492 2,19 12 360 15 15 6 6 39 6,49 29129 Car2 carbonic anhydrase 2 1497 4,04 6 636 33 31 10 10 56,5 6,49 29129 Car2 carbonic anhydrase 2 1499 -2,78 10 646 32 29 10 10 56,5 6,49 29129 Car2 carbonic anhydrase 2 1501 -12,23 5 635 42 37 11 11 62,7 6,49 29129 CRISP1 cysteine-rich secretory protein 1 precursor 1424 3,17 21 279 13 11 6 5 27,9 6,44 28573 H2-Ke6 estradiol 17-beta-dehydrogenase 8 857 -2,65 8 627 14 14 9 9 52,5 6,1 26800 H2-Ke6 estradiol 17-beta-dehydrogenase 8 1423 2 1 899 42 40 10 10 54,8 6,1 26800 H2-Ke6 estradiol 17-beta-dehydrogenase 8 1424 3,17 7 466 12 12 6 6 34 6,1 26800 H2-Ke6 estradiol 17-beta-dehydrogenase 8 1431 -4 10 359 9 9 5 5 29,3 6,1 26800 H2-Ke6 estradiol 17-beta-dehydrogenase 8 1497 4,04 12 504 11 11 6 6 34 6,1 26800 H2-Ke6 estradiol 17-beta-dehydrogenase 8 1501 -12,23 20 334 9 7 5 5 29,3 6,1 26800 GOT1 aspartate aminotransferase cytoplasmic 1033 -1,55 8 567 17 17 10 10 31,5 6,68 46504 glutathione reductase mitochondrial LOC630729 precursor 762 -2 9 688 21 19 11 10 37 8,19 54256 trim69 tripartite motif-containing protein 69 699 2,34 15 713 25 20 17 15 39 6,88 58301 trim69 tripartite motif-containing protein 69 715 2,19 10 774 25 22 16 14 35 6,88 58301 trim69 tripartite motif-containing protein 69 762 -2 6 831 25 21 20 17 44,8 6,88 58301 succinyl-CoA:3-ketoacid-coenzyme A LOC100046138 transferase 2B mitochondrial precursor 667 -2 5 1207 45 40 20 20 58,7 9 57023 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas succinyl-CoA:3-ketoacid-coenzyme A LOC100046138 transferase 2B mitochondrial precursor 676 2 8 1168 47 44 18 17 53,1 9 57023 succinyl-CoA:3-ketoacid-coenzyme A LOC100046138 transferase 2B mitochondrial precursor 699 2,34 3 1424 71 64 20 20 60,4 9 57023 succinyl-CoA:3-ketoacid-coenzyme A LOC100046138 transferase 2B mitochondrial precursor 715 2,19 7 1048 46 41 16 15 46,3 9 57023 succinyl-CoA:3-ketoacid-coenzyme A LOC100046138 transferase 2B mitochondrial precursor 1029 -2 7 572 17 16 9 9 29,8 9 57023 succinyl-CoA:3-ketoacid-coenzyme A LOC100046138 transferase 2B mitochondrial precursor 1043 2 8 717 26 24 11 10 35 9 57023 succinyl-CoA:3-ketoacid-coenzyme A LOC100046138 transferase 2B mitochondrial precursor 1047 -2 3 867 37 35 13 12 39,6 9 57023 succinyl-CoA:3-ketoacid-coenzyme A LOC100046138 transferase 2B mitochondrial precursor 1096 -2 13 494 17 15 8 6 27,5 9 57023 succinyl-CoA:3-ketoacid-coenzyme A LOC100046138 transferase 2B mitochondrial precursor 1158 -18,3 11 558 21 20 10 10 31,2 9 57023 succinyl-CoA:3-ketoacid-coenzyme A LOC100046138 transferase 2B mitochondrial precursor 1497 4,04 7 635 23 21 9 9 29,2 9 57023 succinyl-CoA:3-ketoacid-coenzyme A LOC100046138 transferase 2B mitochondrial precursor 1501 -12,23 2 727 21 19 11 9 33 9 57023 DNPEP aspartyl aminopeptidase isoform b 348 2,33 8 540 20 20 9 9 30,2 6,82 52744 DNPEP aspartyl aminopeptidase isoform b 478 -5,45 8 394 13 11 8 8 26,8 6,82 52744 DNPEP aspartyl aminopeptidase isoform b 507 -1,61 5 778 42 36 12 12 38 6,82 52744 DNPEP aspartyl aminopeptidase isoform b 576 1,65 8 720 23 23 11 11 36 6,82 52744 DNPEP aspartyl aminopeptidase isoform b 594 1,87 10 539 20 20 8 8 28,8 6,82 52744 DNPEP aspartyl aminopeptidase isoform b 630 -1,78 4 890 48 47 14 14 42,1 6,82 52744 DNPEP aspartyl aminopeptidase isoform b 641 -2,19 3 1322 91 84 21 21 59,6 6,82 52744 DNPEP aspartyl aminopeptidase isoform b 667 -2 15 703 30 30 11 11 35,1 6,82 52744 DNPEP aspartyl aminopeptidase isoform b 699 2,34 19 596 19 19 10 10 33,4 6,82 52744 DNPEP aspartyl aminopeptidase isoform b 762 -2 8 749 27 27 13 13 41,6 6,82 52744 serine/threonine-protein phosphatase PP1- PPP1CB beta catalytic subunit 1140 4,63 3 846 37 36 15 15 57,5 5,84 37961 ME1 NADP-dependent malic enzyme isoform 1 348 2,33 3 894 31 30 14 14 39,5 7,16 64540 ME1 NADP-dependent malic enzyme isoform 1 473 -3,03 4 821 35 30 14 12 32 7,16 64540 ME1 NADP-dependent malic enzyme isoform 1 478 -5,45 3 996 41 37 17 16 39,5 7,16 64540

ME1 NADP-dependent malic enzyme isoform 1 568 -3,47 2 1724 94 91 27 27 64,2 7,16 64540 ME1 NADP-dependent malic enzyme isoform 1 576 1,65 2 1790 94 92 28 28 68,7 7,16 64540 lmna prelamin-A/C isoform A 1881 -23,73 7 1253 36 30 24 21 36 6,54 74478 copper homeostasis protein cutC homolog cutC isoform 1 1499 -2,78 11 491 20 18 9 8 53,3 6,95 29384 CTPS CTP synthase 1 471 3,74 3 794 28 24 14 11 29,6 6,14 67324 dsp desmoplakin 676 2 1 4225 185 160 84 69 29,6 6,43 335158 dsp desmoplakin 1881 -23,73 1 4493 174 156 95 84 31,3 6,43 335158 GSTO2 glutathione S-transferase omega-2 1497 4,04 25 253 10 7 7 5 31 7,43 29436 GSTO2 glutathione S-transferase omega-2 1499 -2,78 18 382 19 16 8 8 33,5 7,43 29436 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas cytosolic acyl coenzyme A thioester hydrolase ACOT7 isoform 2 1096 -2 6 604 28 28 8 8 25,9 7,64 42045 cytosolic acyl coenzyme A thioester hydrolase ACOT7 isoform 2 1108 -2,66 5 621 26 25 9 9 29,3 7,64 42045 cytosolic acyl coenzyme A thioester hydrolase ACOT7 isoform 2 1120 2,16 5 476 12 12 8 8 29 7,64 42045 iron-sulfur cluster co-chaperone protein hscB HscB mitochondrial precursor 1599 -2,41 9 432 12 12 8 8 41,5 7 27055 PGK2 phosphoglycerate kinase 2 476 5,43 12 590 19 16 12 10 36,5 6,36 45223 PGK2 phosphoglycerate kinase 2 576 1,65 16 487 14 11 11 9 34,5 6,36 45223 PGK2 phosphoglycerate kinase 2 594 1,87 1 1191 62 58 19 18 61,2 6,36 45223 PGK2 phosphoglycerate kinase 2 667 -2 23 438 12 10 9 7 25,7 6,36 45223 PGK2 phosphoglycerate kinase 2 762 -2 23 340 11 7 9 6 26,6 6,36 45223 PGK2 phosphoglycerate kinase 2 857 -2,65 1 1084 46 44 17 17 54 6,36 45223 NIPSNAP3A nipsnap homolog 3A 1529 -3,69 13 362 16 13 6 5 33,9 8,59 28472 NIPSNAP3A nipsnap homolog 3A 1656 -4,33 5 633 36 33 10 10 62 8,59 28472 NIPSNAP3A nipsnap homolog 3A 1660 -2,87 5 628 36 28 10 10 53,9 8,59 28472 NIPSNAP3A nipsnap homolog 3A 1662 2 5 581 31 26 9 9 51 8,59 28472 protein-L-isoaspartate(D-aspartate) O- pcmt1 methyltransferase 1656 -4,33 6 570 29 25 9 9 43,5 8,4 30493 protein-L-isoaspartate(D-aspartate) O- pcmt1 methyltransferase 1662 2 12 470 13 11 8 6 31,6 8,4 30493 CCT6B T-complex protein 1 subunit zeta-2 593 2,02 9 797 24 22 15 15 34 6,96 58661 CCT6B T-complex protein 1 subunit zeta-2 630 -1,78 3 1124 42 42 20 20 51 6,96 58661 CCT6B T-complex protein 1 subunit zeta-2 641 -2,19 4 1294 41 40 23 23 57,1 6,96 58661 CCT6B T-complex protein 1 subunit zeta-2 667 -2 6 1058 31 30 20 20 45,2 6,96 58661 CCT6B T-complex protein 1 subunit zeta-2 699 2,34 14 716 17 14 15 13 35 6,96 58661 YWHAE 14-3-3 protein epsilon 1881 -23,73 68 364 14 13 6 5 28,6 4,63 29326 G6pd2 glucose-6-phosphate 1-dehydrogenase 2 699 2,34 18 603 23 21 14 12 30 6,52 59569 zona pellucida sperm-binding protein 3 Zp3r receptor precursor 473 -3,03 5 613 27 27 12 12 32 6,88 66819 zona pellucida sperm-binding protein 3 Zp3r receptor precursor 476 5,43 10 634 30 25 11 10 25,4 6,88 66819 zona pellucida sperm-binding protein 3 Zp3r receptor precursor 478 -5,45 4 694 38 33 13 13 33,9 6,88 66819 zona pellucida sperm-binding protein 3 Zp3r receptor precursor 480 20,72 4 725 34 33 13 12 25,2 6,88 66819 zona pellucida sperm-binding protein 3 Zp3r receptor precursor 507 -1,61 7 639 30 29 10 10 26,1 6,88 66819 zona pellucida sperm-binding protein 3 Zp3r receptor precursor 857 -2,65 3 785 51 47 14 14 34 6,88 66819 Cct7 T-complex protein 1 subunit eta 667 -2 9 973 42 42 16 16 39 7,95 60127 Cct7 T-complex protein 1 subunit eta 699 2,34 22 578 18 15 11 10 25,7 7,95 60127 phosphoribosyl pyrophosphate synthase- PRPSAP1 associated protein 1 1113 -2,48 8 494 12 9 9 6 33 8 42783 IL4I1 L-amino-acid oxidase precursor 568 -3,47 11 692 22 21 13 12 25,1 6,28 70347 Orden de Número de Secuencias Número de Cociente Score de la Número de Sequencias de % de cobertura Proteína Nombre de la proteína coincidencia coincidencias significativas de pI Masa spot promedio proteína coincidencias proteínas de la proteína en el spot significativas proteínas IL4I1 L-amino-acid oxidase precursor 576 1,65 5 957 33 31 17 17 35 6,28 70347 gpi1 glucose-6-phosphate isomerase 593 2,02 11 683 21 19 13 13 29,9 8,14 62955 gpi1 glucose-6-phosphate isomerase 641 -2,19 9 744 30 26 12 11 29,7 8,14 62955 gpi1 glucose-6-phosphate isomerase 667 -2 11 881 46 43 14 14 35 8,14 62955 gpi1 glucose-6-phosphate isomerase 676 2 11 888 50 45 15 15 36 8,14 62955 gpi1 glucose-6-phosphate isomerase 699 2,34 8 970 47 40 15 15 37 8,14 62955 serine/threonine-protein phosphatase 2A ppp2r4 activator 1104 -2 6 395 15 9 8 5 30 5,95 36972 glb1l beta-galactosidase-1-like protein precursor 699 2,34 9 842 36 35 14 14 25,1 6,79 73460 PSMA8 proteasome subunit alpha type-7-like 1499 -2,78 24 278 6 6 5 5 27,6 8,81 28020 PSMA8 proteasome subunit alpha type-7-like 1501 -12,23 13 453 13 13 8 8 42,8 8,81 28020 LAP3 cytosol aminopeptidase 476 5,43 14 548 19 18 11 10 25,4 7,62 56505 LAP3 cytosol aminopeptidase 715 2,19 2 1304 60 57 21 19 55,9 7,62 56505 Rnls renalase isoform 1 1033 -1,55 28 238 9 8 5 5 26,7 7,14 33892 Rnls renalase isoform 1 1043 2 17 431 15 13 7 6 40 7,14 33892 Rnls renalase isoform 1 1047 -2 11 483 16 16 7 7 38 7,14 33892 fructose-bisphosphate aldolase A isoform 1 Aldoa precursor 857 -2,65 2 888 41 37 18 15 55 8 45548 fructose-bisphosphate aldolase A isoform 1 Aldoa precursor 1029 -2 2 1037 41 36 20 18 61,2 8 45548 aldoa1a aldolase 1 A retrogene 1 835 -4 3 1094 51 48 17 17 58,9 6,61 45885 aldoa1a aldolase 1 A retrogene 1 857 -2,65 4 762 31 30 14 13 37,5 6,61 45885 aldoa1a aldolase 1 A retrogene 1 1029 -2 6 637 30 25 12 12 34,6 6,61 45885 aldoa1a aldolase 1 A retrogene 1 1033 -1,55 6 632 43 38 11 10 30,8 6,61 45885 aldoa1a aldolase 1 A retrogene 1 1043 2 9 677 31 28 11 10 32,5 6,61 45885 aldoa1a aldolase 1 A retrogene 1 1047 -2 4 694 44 38 13 12 38 6,61 45885

Pkm pyruvate kinase isozymes M1/M2 isoform 2 555 -2,11 3 844 32 31 13 13 28,2 6,69 58461

Pkm pyruvate kinase isozymes M1/M2 isoform 2 568 -3,47 3 1699 70 67 25 25 56,1 6,69 58461

Pkm pyruvate kinase isozymes M1/M2 isoform 2 576 1,65 1 2075 100 95 30 29 67 6,69 58461

Pkm pyruvate kinase isozymes M1/M2 isoform 2 593 2,02 1 2024 93 89 29 29 66,5 6,69 58461

Pkm pyruvate kinase isozymes M1/M2 isoform 2 630 -1,78 2 1671 63 60 26 25 60,3 6,69 58461

Pkm pyruvate kinase isozymes M1/M2 isoform 2 641 -2,19 2 1685 66 65 25 25 57,6 6,69 58461

Pkm pyruvate kinase isozymes M1/M2 isoform 2 667 -2 1 2335 113 107 33 33 69,5 6,69 58461

Pkm pyruvate kinase isozymes M1/M2 isoform 2 699 2,34 7 1017 31 29 17 16 41,4 6,69 58461 Prdx6 PREDICTED: peroxiredoxin-6-like 1599 -2,41 2 893 46 43 13 12 67 5,71 24999 Tuba1c PREDICTED: tubulin alpha-1C chain-like 1096 -2 23 381 14 12 7 7 36,4 4,79 28145 Tuba1c PREDICTED: tubulin alpha-1C chain-like 1113 -2,48 6 507 15 14 8 8 50,6 4,79 28145 Tuba1c PREDICTED: tubulin alpha-1C chain-like 1499 -2,78 20 362 13 12 6 6 36 4,79 28145