EUROPEAN JOURNAL OF ENTOMOLOGYENTOMOLOGY ISSN (online): 1802-8829 Eur. J. Entomol. 114: 407–415, 2017 http://www.eje.cz doi: 10.14411/eje.2017.052 ORIGINAL ARTICLE Tandem duplication of two tRNA genes in the mitochondrial genom e of Tagiades vajuna (Lepidoptera: Hesperiidae) FANG-FANG LIU 1, *, YI -PING LI 1, 2, *, IVAN JA KOVLIĆ 3 and XIANG-QUN YU AN 1, ** 1 Ke y Laboratory of Plant Protection Resources and Pest Management, Ministry of Education; College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China; e-mails:
[email protected] (F.-F.L.), ly
[email protected] (Y.-P.L), yua
[email protected] (X.-Q.Y.) 2 Key Laboratory of Applied Entomology, Northwest A&F University, Yangling, Shaanxi 712100, China 3 De partment of Fisheries, Beekeeping, Game Management and Special Zoology, Fa culty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Za greb, Croatia; e-mail: i
[email protected] Key words. Lepidoptera, Hesperiidae, Pyrginae, Eudaminae, Tag iades vajuna, mit ochondrial phylogenomics, tRNA duplication, butterfl ies, skippers Abstract. To explore the debated phylogenetic relationship of two Hesperiidae subfamilies, Pyrginae and Eudaminae, and con- tribute to the understanding of the evolution of mitogenomic architecture in butterfl ies, we sequenced the complete mitogenome of Tag iades vajuna. The mitogenome is a typical circular duplex molecule of 15,359 bp. Apart from the standard 22 tRNAs, it has a tandem duplication of trnS(AGN) and trnE, which is unique in lepidopteran insects. Comparison with Ctenoptilum vasava indi- cates that the trnS1 duplication is not an ancestral state shared with other species of Tagiadini.