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Anti-Proteus Vulgaris Antibody (SAB4200817)

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Anti-Proteus Vulgaris Antibody (SAB4200817) Anti-Proteus vulgaris antibody produced in rabbit IgG fraction of antiserum Product Number SAB4200817 Product Description P. vulgaris have a number of putative virulence factors, Anti-Proteus vulgaris antibody is developed in rabbits including the secreted hemolytic haemolysin and using UV-killed P. vulgaris OX19 bacteria (ATCC urease, which have been suggested to contribute to 6380). Whole antiserum is purified using protein A host cell invasion and cytotoxicity, and bacterial ability immobilized on agarose to provide the IgG fraction of to invade uroepithelial cells. 4 P. vulgaris, P. mirabilis, antiserum. and P. penneri harbor resistance to -lactam antibiotics as they are capable of producing inducible Anti-Proteus vulgaris antibody recognizes P. vulgaris -lactamases that hydrolyze primary and whole extract and P. vulgaris LPS. The antibody also extended-spectrum penicillins and cephalosporins. 5-7 recognizes an additional 70 kDa band, suspected to be bacterial HSP70 (DNAK) in whole extracts of Reagent P. mirabilis, P. gingivalis, E.coli K-12, P.aeruginosa, Supplied as a solution in 0.01 M phosphate buffered S. flexneri, S. enterica and E. faecalis, but it has no saline, pH 7.4, containing 15 mM sodium azide as a crossreactivity with P. mirabilis LPS. The antibody may preservative. be used in various immunochemical techniques including immunoblotting and ELISA. Detection of the Precautions and Disclaimer P. vulgaris LPS bands by Immunoblotting is specifically This product is for R&D use only, not for drug, inhibited by the immunogen. household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe Proteus vulgaris is a Gram-negative rod-shaped handling practices. bacterium belonging to the Enterobacteriaceae family. Members of the genus Proteus (Proteus spp.), which Storage/Stability also includes Proteus mirabilis, Proteus penneri, and For continuous use, store at 2─8 C for up to one Proteus hauseri, originally characterized by their ability month. For extended storage, freeze in working to swarm on solid surfaces, are widespread in the aliquots. Repeated freezing and thawing is not environment and the gastrointestinal tract of humans recommended. If slight turbidity occurs upon prolonged and animals, and known to be an opportunistic storage, clarify the solution by centrifugation before pathogens isolated from urine, wounds, and other use. Working dilution samples should be discarded if clinical sources. not used within 12 hours. The Proteus spp. bacteria are distinguished by their Product Profile reactions for indole production, salicin fermentation, Immunoblotting: a working dilution of 1:20,000-1:40,000 1-3 and aesculin hydrolysis. P. vulgaris produces indole is recommended using Proteus vulgaris LPS. which differentiates it from the indole-negative 1-3 P. mirabilis and P. penneri. Proteus spp. bacteria Indirect ELISA: a working dilution of 1:20,000-1:40,000 may also be found in soil or water habitats where they is recommended using dead Proteus vulgaris bacteria are often regarded as indicators of fecal pollution and a for coating. contamination threat for potential water or seafood 1 poisoning. Note: In order to obtain best results in different techniques and preparations, it is recommended to In the Proteus spp. group, P. mirabilis is encountered in determine optimal working concentration by titration the community and causes the majority of urinary tract test. Proteus spp. infections, whereas P. vulgaris and P. penneri are less common and mainly associated with nosocomial none urinary infections.2,4 References 1. Drzewiecka, D., Microb. Ecol., 72, 741-758 4. Ghaidaa, M. et al., N. Y. Sci. J., 6, 8-14 (2013). (2016). 5. Bahashwan, S.A., and Shafey, H.M., Euro. Sci. 2. O'Hara, C.M. et al., Int. J. Syst. Evol. Microbiol., J., 9, 188-202 (2013). 50, 1869-75 (2000). 6. Pal, N. et al., Ann. Med. Health Sci. Res., 6, 3. O'Hara, C.M. et al., Clin. Microbiol. Rev., 13, 267-73 (2016). 534-46 (2000). AI,DR,OKF,MAM 10/18-1 2018 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip..
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