DOCSLIB.ORG

Anti-Proteus Vulgaris LPS Antibody (SAB4200850)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Anti-Proteus Vulgaris LPS Antibody (SAB4200850) Anti-Proteus vulgaris LPS antibody Mouse monoclonal, Clone P.vul-111 purified from hybridoma cell culture Product Number SAB4200850 Product Description In the Proteus spp. group, P. mirabilis is encountered in Monoclonal Anti-Proteus vulgaris LPS antibody (mouse the community and causes the majority of urinary tract IgM isotype) is derived from the P.vul-111 hybridoma, Proteus spp. infections, whereas P. vulgaris and produced by the fusion of mouse myeloma cells and P. penneri are less common and mainly associated with splenocytes from a mouse immunized with nosocomial none urinary infections.2,4 UV-inactivated P. vulgaris OX19 bacteria (ATCC 6380) as immunogen. The isotype is determined by ELISA P. vulgaris has a number of putative virulence factors, using Mouse Monoclonal Antibody Isotyping Reagents including the secreted hemolytic haemolysin and (Product Number ISO2). The antibody is purified from urease, which has been suggested to contribute to host culture supernatant of hybridoma cells. cell invasion, cytotoxicity, and bacterial ability to invade uroepithelial cells.4 P. vulgaris, P. mirabilis and Monoclonal Anti-Proteus vulgaris LPS specifically P. penneri harbor resistance to -lactam antibiotics as it recognizes P. vulgaris whole extract and P. vulgaris is capable of producing inducible -lactamases that LPS, the antibody has no cross reactivity with whole hydrolyze primary and extended-spectrum penicillins extract of Proteus mirabilis, P. gingivalis, E. coli, and cephalosporins. 5-6 Pseudomonas aeruginosa, Shigella flexneri, Staphylococcus aureus, or Salmonella enterica. The Reagent antibody is recommended to be used in various Supplied as a solution in 0.01 M phosphate buffered immunological techniques, including immunoblot and saline, pH 7.4, containing 15 mM sodium azide as a ELISA. Detection of the P. vulgaris band by preservative. immunoblotting is specifically inhibited by the immunogen. Antibody Concentration: 1.0 mg/mL Proteus vulgaris, a Gram-negative rod-shaped Precautions and Disclaimer bacterium, belongs to the Enterobacteriaceae family. For R&D use only. Not for drug, household, or other Members of the genus Proteus (Proteus spp.), which uses. Please consult the Safety Data Sheet for also includes Proteus mirabilis, Proteus penneri, and information regarding hazards and safe handling Proteus hauseri, originally characterize by their ability practices. to swarm on solid surfaces, are widespread in the environment and the gastrointestinal tract of human Storage/Stability and animals and known to be an opportunistic For continuous use, store at 2–8 C for up to one pathogens isolated from urine, wounds, and other month. For extended storage, freeze in working clinical sources. The Proteus spp. bacteria, are aliquots. Repeated freezing and thawing is not distinguished by their reactions for indole production, recommended. If slight turbidity occurs upon prolonged 1-3 salicin fermentation, and aesculin hydrolysis. storage, clarify the solution by centrifugation before P. vulgaris produces indole, which differentiates it from use. Working dilution samples should be discarded if 1-3 the indole-negative P. mirabilis and P. penneri. not used within 12 hours. Proteus spp. bacteria may also be found in soil or water Product Profile habitats where they often regarded as indicators of Immunoblotting: a working of concentration fecal pollution and a contamination threat for potential 0.05-0.1 g/mL is recommended using dead 1 water or seafood poisoning. P. vulgaris bacteria lysate. Note: In order to obtain best results in various techniques and preparations, it is recommended to determine optimal working dilutions by titration test. References 1. Drzewiecka, D., Microb. Ecol., 72, 741-758 4. Ghaidaa, M., et al., N.Y. Sci. J., 6, 8-14 (2013). (2016). 5. Bahashwan, S.A., and Shafey, H.M., Euro. Sci. 2. O'Hara, C.M., et al., Int. J. Syst. Evol. Microbiol., J., 9, 188-202 (2013). 50, 1869-75 (2000). 6. Pal, N., et al., Ann. Med. Health Sci. Res., 6, 3. O'Hara, C.M., et al., Clin. Microbiol. Rev., 13, 267-73 (2016). 534-46 (2000). VS,DR,OKF,MAM 08/19-1 2019 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip. .
Load more

Recommended publications
  • Proteus Vulgaris
    48 Monte Carlo Crescent Kyalami Business Park Kyalami, Johannesburg, 1684, RSA Tel: +27 (0)11 463 3260 Fax: + 27 (0)86 557 2232 Email: [email protected] www.thistle.co.za Please read this section first The HPCSA and the Med Tech Society have confirmed that this clinical case study, plus your routine review of your EQA reports from Thistle QA, should be documented as a “Journal Club” activity. This means that you must record those attending for CEU purposes. Thistle will not issue a certificate to cover these activities, nor send out “correct” answers to the CEU questions at the end of this case study. The Thistle QA CEU No is: MT- 16/009 Each attendee should claim THREE CEU points for completing this Quality Control Journal Club exercise, and retain a copy of the relevant Thistle QA Participation Certificate as proof of registration on a Thistle QA EQA. MICROBIOLOGY LEGEND CYCLE 41 ORGANISM 3 Proteus Vulgaris Proteus Vulgaris is a rod shaped Gram-Negative chemoheterotrophic bacterium. The size of the individual cells varies from 0.4 to 0.6 micrometers by 1.2 to 2.5 micrometers. P. vulgaris possesses peritrichous flagella, making it actively motile. It inhabits the soil, polluted water, raw meat, gastrointestinal tracts of animals and dust. In humans, Proteus species most frequently cause urinary tract infections, but can also produce severe abscesses and is widely associated with nosocomial infections. Isolation of Organism With basic microbiological technique, samples believed to contain P. vulgaris are first incubated on nutrient agar to form colonies. To test the Gram-Negative and oxidase-negative characteristics of Enterobacteriaceae, Gram stains and oxidase tests are performed.
    [Show full text]
  • Helicobacter Pylori Infection and Its Potential Association with Idiopathic
    Journal of Immunology and Infectious Diseases Volume 2 | Issue 2 ISSN: 2394-6512 Research Article Open Access Helicobacter pylori Infection and its Potential Association with Idiopathic Hypercalciuric Urolithiasis in Pediatric Patients Ali AM*1, Abdelaziz SS2 and Elkhatib WF3,4 1Department of Pediatrics, International Islamic Center for Population Studies and Research, Faculty of Medicine, Al-Azhar University, Cairo, Egypt 2Department of Urology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt 3Department of Microbiology & Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St. Abbassia, Cairo, Egypt 4Department of Pharmacy Practice, School of Pharmacy, Hampton University, Kittrell Hall Hampton, Virginia, USA *Corresponding author: Ali AM, Department of Pediatrics, Faculty of Medicine, Al-Azhar University, Cairo, Egypt, Postal Code: 31991, Dr. Noor Mohammad Khan General Hospital, Fax: +966713221417, Tel: +966556847829, E-mail: [email protected] Citation: Ali AM, Abdelaziz SS, Elkhatib WF (2014) Helicobacter pylori Infection and its Potential Association with Idiopathic Hypercalciuric Urolithiasis in Pediatric Patients. J Immunol Infect Dis 2(2): 202. doi: 10.15744/2394-6512.1.203 Received Date: September 24, 2014 Accepted Date: January 05, 2015 Published Date: January 09, 2015 Abstract Objectives: To evaluate the role of Helicobacter pylori infection in the pathogenesis of idiopathic hypercalciuric urolithiasis. Design & Setting: Randomized longitudinal controlled study was carried out at Al-Azhar University Hospitals in Cairo and Dommiat, Egypt as well as at Noor Khan General Hospital in Hafer Al-Baten, Saudi Arabia. Participants: A total of 150 patients categorized into 100 cases (urolithiasis-positive) with urinary stone disease, aged from 5 to 18 years, and met the characteristics of idiopathic urolithiasis in children as well as 50 controls (urolithiasis-negative) that had relatively similar demographic criteria except for idiopathic urolithiasis.
    [Show full text]
  • Transposon Mutagenesis in Proteus Mirabilist
    JOURNAL OF BACTERIOLOGY, OCt. 1991, p. 6289-6293 Vol. 173, No. 19 0021-9193/91/196289-05$02.00/0 Copyright © 1991, American Society for Microbiology NOTES Transposon Mutagenesis in Proteus mirabilist ROBERT BELAS,1,2* DEBORAH ERSKINE,1 ANID DAVID FLAHERTY1 Center of Marine Biotechnology, The University of Maryland, 600 East Lombard Street, Baltimore, Maryland 21202,1* and Department ofBiological Sciences, University of Maryland Baltimore County, Baltimore, Maryland 212282 Received 21 June 1991/Accepted 24 July 1991 A technique of transposon mutagenesis involving the use of TnS on a suicide plasmid was developed for Proteus mirabilis. Analysis of the resulting exconjugants indicated that TnS transposed in P. mirabilis at a frequency of ca. 4.5 x 10-6 per recipient cell. The resulting mutants were stable and retained the transposon-encoded antibiotic resistance when incubated for several generations under nonselective conditions. The frequency of auxotrophic mutants in the population, as well as DNA-DNA hybridization to transposon sequences, confirmed that the insertion of the transposon was random and the Proteus chromosome did not contain significant insertional hot spots of transposition. Approximately 35% of the mutants analyzed possessed plasmid-acquired ampicillin resistance, although no extrachromosomal plasmid DNA was found. In these mutants, insertion of the Tn5 element and a part or all of the plasmid had occurred. Application of this technique to the study of swarmer cell differentiation in P. mirabilis is discussed. Proteus mirabilis is a motile gram-negative bacterium with Media and growth conditions. Escherichia coli and P. the unique ability to move over agar surfaces by a locomo- mirabilis strains were grown in L broth (10 g of tryptone, 5 tive process referred to as swarming motility (12).
    [Show full text]
  • Uncommon Pathogens Causing Hospital-Acquired Infections in Postoperative Cardiac Surgical Patients
    Published online: 2020-03-06 THIEME Review Article 89 Uncommon Pathogens Causing Hospital-Acquired Infections in Postoperative Cardiac Surgical Patients Manoj Kumar Sahu1 Netto George2 Neha Rastogi2 Chalatti Bipin1 Sarvesh Pal Singh1 1Department of Cardiothoracic and Vascular Surgery, CN Centre, All Address for correspondence Manoj K Sahu, MD, DNB, Department India Institute of Medical Sciences, Ansari Nagar, New Delhi, India of Cardiothoracic and Vascular Surgery, CTVS office, 7th floor, CN 2Infectious Disease, Department of Medicine, All India Institute of Centre, All India Institute of Medical Sciences, New Delhi-110029, Medical Sciences, Ansari Nagar, New Delhi, India India (e-mail: [email protected]). J Card Crit Care 2020;3:89–96 Abstract Bacterial infections are common causes of sepsis in the intensive care units. However, usually a finite number of Gram-negative bacteria cause sepsis (mostly according to the hospital flora). Some organisms such as Escherichia coli, Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus are relatively common. Others such as Stenotrophomonas maltophilia, Chryseobacterium indologenes, Shewanella putrefaciens, Ralstonia pickettii, Providencia, Morganella species, Nocardia, Elizabethkingia, Proteus, and Burkholderia are rare but of immense importance to public health, in view of the high mortality rates these are associated with. Being aware of these organisms, as the cause of hospital-acquired infections, helps in the prevention, Keywords treatment, and control of sepsis in the high-risk cardiac surgical patients including in ► uncommon pathogens heart transplants. Therefore, a basic understanding of when to suspect these organ- ► hospital-acquired isms is important for clinical diagnosis and initiating therapeutic options. This review infection discusses some rarely appearing pathogens in our intensive care unit with respect to ► cardiac surgical the spectrum of infections, and various antibiotics that were effective in managing intensive care unit these bacteria.
    [Show full text]
  • Antibiotic Use Guidelines for Companion Animal Practice (2Nd Edition) Iii
    ii Antibiotic Use Guidelines for Companion Animal Practice (2nd edition) iii Antibiotic Use Guidelines for Companion Animal Practice, 2nd edition Publisher: Companion Animal Group, Danish Veterinary Association, Peter Bangs Vej 30, 2000 Frederiksberg Authors of the guidelines: Lisbeth Rem Jessen (University of Copenhagen) Peter Damborg (University of Copenhagen) Anette Spohr (Evidensia Faxe Animal Hospital) Sandra Goericke-Pesch (University of Veterinary Medicine, Hannover) Rebecca Langhorn (University of Copenhagen) Geoffrey Houser (University of Copenhagen) Jakob Willesen (University of Copenhagen) Mette Schjærff (University of Copenhagen) Thomas Eriksen (University of Copenhagen) Tina Møller Sørensen (University of Copenhagen) Vibeke Frøkjær Jensen (DTU-VET) Flemming Obling (Greve) Luca Guardabassi (University of Copenhagen) Reproduction of extracts from these guidelines is only permitted in accordance with the agreement between the Ministry of Education and Copy-Dan. Danish copyright law restricts all other use without written permission of the publisher. Exception is granted for short excerpts for review purposes. iv Foreword The first edition of the Antibiotic Use Guidelines for Companion Animal Practice was published in autumn of 2012. The aim of the guidelines was to prevent increased antibiotic resistance. A questionnaire circulated to Danish veterinarians in 2015 (Jessen et al., DVT 10, 2016) indicated that the guidelines were well received, and particularly that active users had followed the recommendations. Despite a positive reception and the results of this survey, the actual quantity of antibiotics used is probably a better indicator of the effect of the first guidelines. Chapter two of these updated guidelines therefore details the pattern of developments in antibiotic use, as reported in DANMAP 2016 (www.danmap.org).
    [Show full text]
  • Investigating the Biological and Mechanical Performance of Cranberry-Modified Silicone Materials for Use in Implantable Medical Devices
    Investigating the biological and mechanical performance of cranberry-modified silicone materials for use in implantable medical devices By Louis Briggs Department of Chemical Engineering McGill University, Montreal August 2014 A thesis submitted to McGill University in partial fulfillment of the requirements of the degree of Master of Engineering © Louis Briggs 2014 Abstract Catheter associated urinary tract infections (CAUTIs) are the second most common type of hospital acquired infection in North America with over a million cases reported each year. This high incidence rate, together with the dissemination of antibiotic resistant uropathogens has resulted in much interest in the development of preventative measures within the research community. The consumption of Vaccinium macrocarpon, the North American cranberry, has been linked with the prevention of bacterial infections in the urinary tract for over 100 years. The McGill Biocolloids and Surfaces Laboratory has shown that cranberry derived materials (CDMs) can inhibit bacterial adherence to surfaces, impair bacterial motility, and influence various bacterial virulence functions. It is therefore hypothesized that cranberries and CDMs could prevent the development of CAUTIs via the disruption of various stages of pathogenesis. Herein, we report on the bioperformance of cranberry impregnated biomedical grade silicone against the biofilms formed by Proteus mirabilis, a clinically relevant uropathogen. In addition, the mechanical performance of this cranberry-silicone hybrid material is studied. First, the release of bioactive cranberry materials into an aqueous environment is demonstrated in lysogeny broth using a spectrophotometric method. Second, the ultimate tensile strength, elongation at break, storage modulus, and contact angle of CDM impregnated silicone are reported. Finally, the ability of P.
    [Show full text]
  • Original Article COMPARISON of MAST BURKHOLDERIA CEPACIA, ASHDOWN + GENTAMICIN, and BURKHOLDERIA PSEUDOMALLEI SELECTIVE AGAR
    European Journal of Microbiology and Immunology 7 (2017) 1, pp. 15–36 Original article DOI: 10.1556/1886.2016.00037 COMPARISON OF MAST BURKHOLDERIA CEPACIA, ASHDOWN + GENTAMICIN, AND BURKHOLDERIA PSEUDOMALLEI SELECTIVE AGAR FOR THE SELECTIVE GROWTH OF BURKHOLDERIA SPP. Carola Edler1, Henri Derschum2, Mirko Köhler3, Heinrich Neubauer4, Hagen Frickmann5,6,*, Ralf Matthias Hagen7 1 Department of Dermatology, German Armed Forces Hospital of Hamburg, Hamburg, Germany 2 CBRN Defence, Safety and Environmental Protection School, Science Division 3 Bundeswehr Medical Academy, Munich, Germany 4 Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Jena, Germany 5 Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Hamburg, Germany 6 Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Rostock, Germany 7 Department of Preventive Medicine, Bundeswehr Medical Academy, Munich, Germany Received: November 18, 2016; Accepted: December 5, 2016 Reliable identification of pathogenic Burkholderia spp. like Burkholderia mallei and Burkholderia pseudomallei in clinical samples is desirable. Three different selective media were assessed for reliability and selectivity with various Burkholderia spp. and non- target organisms. Mast Burkholderia cepacia agar, Ashdown + gentamicin agar, and B. pseudomallei selective agar were compared. A panel of 116 reference strains and well-characterized clinical isolates, comprising 30 B. pseudomallei, 20 B. mallei, 18 other Burkholderia spp., and 48 nontarget organisms, was used for this assessment. While all B. pseudomallei strains grew on all three tested selective agars, the other Burkholderia spp. showed a diverse growth pattern. Nontarget organisms, i.e., nonfermentative rod-shaped bacteria, other species, and yeasts, grew on all selective agars.
    [Show full text]
  • Clinical Antibiotic Guidelines†
    CLINICAL ANTIBIOTIC GUIDELINES† ACYCLOVIR IV*/PO *RESTRICTED TO ANTIBIOTIC FORM Predictable activity: Unpredictable activity: No activity: Herpes Simplex Cytomegalovirus Epstein Barr Virus Herpes Zoster Indicated: IV: 1. Therapy for suspected or documented Herpes simplex encephalitis 2. Therapy for suspected or documented Herpes simplex infection of a newborn or immunocompromised patient 3. Therapy for primary varicella infection in immunocompromised patients 4. Therapy for severe or disseminated varicella-zoster infections in immunocompromised or immunocompetent patient 5. Therapy for primary genital herpes with neurologic complications Oral: 1. Therapy for primary Herpes simplex infections (oral/genital) 2. Suppressive (preventative) therapy for recurrent (³ 6 episodes/year) severe Herpes simplex infections (oral/genital) 3. Episodic therapy for recurrent (³ 6 episodes/year) Herpes simplex genital infections (initiate within 24 hours of prodrome onset) 4. Prophylaxis for HSV in bone marrow transplants where patient is seropositive 5. Therapy and suppressive therapy for Eczema Herpeticum 6. Therapy for varicella-zoster infections in immunocompetent and immunocompromised patients (if not severe) 7. Therapy for primary varicella infections in pregnancy 8. Therapy for varicella in immunocompetent patients > 13 years old (initiate within 24 hours of rash onset) 9. Therapy for varicella in patients < 13 years old (initiate within 24 hours of rash onset) if there is a chronic cutaneous or pulmonary disorder, long term salicylate therapy, or short, intermittent or aerosolized corticosteroid use Not Indicated: 1. Therapy for acute Epstein-Barr infections (acute mononucleosis) 2. Therapy for documented CMV infections CLINICAL ANTIBIOTIC GUIDELINES† AMIKACIN RESTRICTED TO ANTIBIOTIC FORM Predictable activity: Unpredictable activity: No activity: Enterobacteriaceae Staphylococcus spp Streptococcus spp Pseudomonas spp Enterococcus spp some Mycobacterium spp Alcaligenes spp Anaerobes Indicated: 1.
    [Show full text]
  • The Enterotube System in the Identification of Enterohacteriaceae and Yersinia
    13 February 1974 S.A. MEDICAL JOURNAL 257 (Supplement-South African Journal of Laboratory and Clinical Medicine) LCM 9 The Enterotube System in the Identification of Enterohacteriaceae and Yersinia M. H. FINLAYSON, B. GIBBS SUMMARY Dulcitol agar for detection of acid production, absent in Proteus sp., most Enterobacters sp., Hafnia and Serratia, A trial of the Enterotube system for the identification of A rizona and Edwardsiella. Enterobacteriaceae and a comparison with the methods Urea agar for detection of urea cleavage, absent in at present in use in the Tygerberg Hospital Microbiology Escherichia, Shigella, Providencia, Enterobacter aerogenes, Laboratory, were carried out. One hunded cultures be­ Hafnia, Salmonella, A rizona and Edwardsiella. longing to the family Enterobacteriaceae including Phenylalanine agar for detection of phenylalanine Escherichia coli, Proteus mirabilis, Proteus vulgaris, deaminase by production of a green colouration after Proteus morgani, Proteus rettgeri, Providencia, Edward­ addition of ferric chloride solution. This reaction is posi­ siella, Shigella, Klebsiella, Enterobacter, Serratis, Sal­ tive only in the Proteus and Pro\'idencia groups. monella, Citrobacter and Arizona were tested with con­ ventional procedures and also with the Enterotube. The Hydrogen sulphide and indole agar for detection of conventional procedures, designed to identify the hydrogen sulphide and indole, the latter being detected organisms within 24 hours after isolation, were used. Three after the addition of Kovac's reagent to the compartment. of the cultures examined were not identified by the Hydrogen sulphide is produced by some Proteus strains Enterotube technique when their identity was established and by Salmonella, Citrobacter, Arizona and Edwardsie/la by routine conventional methods. These were non­ while indole is produced by Escherichia, .some Proteus urease-producing non-motile organisms which may have strains, Providencia and Edwardsiella.
    [Show full text]
  • Pdf 355.26 K
    Beni-Suef BS. VET. MED. J. JULY 2010 VOL.20 NO.2 P.16-24 Veterinary Medical Journal An approach towards bacterial pathogens of zoonotic importance harbored by commensal rodents prevalent in Beni- Suef Governorate W. H. Hassan1, A. E. Abdel-Ghany2 1Department of Bacteriology, Mycology and Immunology, and 2 Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt This study was conducted in the period July 2009 through June 2010 to determine the role of commensal rodents in transmitting bacterial pathogens to man in Beni-Suef Governorate, Egypt. A total of 50 rats of various species were selected from both urban and rural areas at different localities. In the laboratory, rodent species were identified and bacteriological examination was performed. Seven types of samples were cultured from external and internal body parts of each rat. The identified rodent spp. included Rattus norvegicus (16%), Rattus rattus rattus (42%) and Rattus rattus frugivorus (42%). The results demonstrated that S. aureus, S. lentus, S. sciuri and S. xylosus were isolated from the examined rats at percentages of 8, 2, 6 and 6 %, respectively. Moreover, E. durans (2%), E. faecalis (12%), E. faecium (24%), E. gallinarum (4%), Aerococcus viridans (12%) and S. porcinus (2%) in addition to Lc. lactis lactis (4%), Leuconostoc sp. (2%) and Corynebacterium kutscheri (8%) were also harbored by the screened rodents. On the other hand, S. arizonae, E. coli, E. cloacae and E. sakazakii were isolated from the examined rats at percentages of 4, 8, 4 and 6 %, respectively. Besides, Proteus mirabilis (6%), Proteus vulgaris (2%), Providencia rettgeri (6%), P.
    [Show full text]
  • Worldwide Links Between Proteus Mirabilis and Rheumatoid Arthritis
    al of Arth rn ri u ti o s J Journal of Arthritis Wilson et al., J Arthritis 2015, 4:1 10.4172/2167-7921.1000142 ISSN: 2167-7921 DOI: Review Open access Worldwide Links between Proteus mirabilis and Rheumatoid Arthritis Clyde Wilson1*, Taha Rashid2 and Alan Ebringer2 1Department of Pathology, King Edward VII Memorial Hospital, Paget DV 07, Bermuda 2Analytical Sciences Group, King’s College London, Stamford Road, London SE1 9NN, UK *Corresponding author: Dr. Clyde Wilson, Department of Pathology, King Edward VII Memorial Hospital, Paget DV 07, Bermuda, USA, Tel: +1-4412391011; Fax: +1-4412392193; Email: [email protected] Rec date: November 26, 2014; Acc date: January 9, 2015; Pub date: January 15, 2015 Copyright: © 2015 Wilson C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract Rheumatoid arthritis (RA) is a systemic and arthritic autoimmune disease affecting millions of people throughout the world. During the last 4 decades extensive data indicate that subclinical urinary tract infection by Proteus mirabilis has a role in the aetiopathogenesis of RA based on cross-reactivity or molecular mimicry between Proteus haemolysin and RA-associated HLA-DRB1 alleles as well as between Proteus urease and type XI collagen. Studies from 15 countries have shown that antibodies against Proteus microbes were elevated significantly in patients with active RA in comparison to healthy and non-RA disease controls. Proteus microbes could also be isolated more frequently in the urine of patients with RA than in controls.
    [Show full text]
  • Study of Some Virulence Factors of Proteus Mirabilis Isolated from Urinary Stones Patients
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by International Institute for Science, Technology and Education (IISTE): E-Journals Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.5, No.23, 2015 Study of Some Virulence Factors of Proteus mirabilis Isolated from Urinary Stones Patients Mohanad J. Al-Dawah 1* Adnan H. Al-Hamadany 2 Eman M. Al-Jarallah 3 1. Al-Qasim Green University, College of Biotechnology-Genetic Engineering Department, Al-Qasim city, Babylon, Iraq 2. Al-Qadissiya University, College of Medicine-Microbiology Department 3. Babylon University, College of Science-Biology Department Abstract A total of 125 specimens of stones and urine were collected from urinary stone patients from (June to December, 2012). According to primary identification, which based on macroscopic and microscopic characteristics and biochemical tests, 25 (20%) and 100 (80%) of isolates were identified as Proteus and non-Proteus , respectively. The 25 Proteus isolates were finally identified as Proteus mirabilis based on Vitek 2 system and polymerase chain reaction (PCR) technique by using target gene 16S rRNA . The multiple logistic regressions results showed that the age ˃ 40 years old was a risk factors that significantly associated with increased incidence of P. mirabilis in urinary stone patients, as P = 0.02, and Odd’s ratio (OR) was 4.889 (1.7-14.057), while in relation to gender, the analysis revealed that they were statistically non-significant as OR was 1.174 (0.488-2.822) as well as P=0.720.
    [Show full text]