Anti-Proteus vulgaris LPS antibody Mouse monoclonal, Clone P.vul-111 purified from hybridoma cell culture

Product Number SAB4200850

Product Description In the Proteus spp. group, P. mirabilis is encountered in Monoclonal Anti-Proteus vulgaris LPS antibody (mouse the community and causes the majority of urinary tract IgM isotype) is derived from the P.vul-111 hybridoma, Proteus spp. infections, whereas P. vulgaris and produced by the fusion of mouse myeloma cells and P. penneri are less common and mainly associated with splenocytes from a mouse immunized with nosocomial none urinary infections.2,4 UV-inactivated P. vulgaris OX19 bacteria (ATCC 6380) as immunogen. The isotype is determined by ELISA P. vulgaris has a number of putative virulence factors, using Mouse Monoclonal Antibody Isotyping Reagents including the secreted hemolytic haemolysin and (Product Number ISO2). The antibody is purified from urease, which has been suggested to contribute to host culture supernatant of hybridoma cells. cell invasion, cytotoxicity, and bacterial ability to invade uroepithelial cells.4 P. vulgaris, P. mirabilis and Monoclonal Anti-Proteus vulgaris LPS specifically P. penneri harbor resistance to -lactam antibiotics as it recognizes P. vulgaris whole extract and P. vulgaris is capable of producing inducible -lactamases that LPS, the antibody has no cross reactivity with whole hydrolyze primary and extended-spectrum penicillins extract of Proteus mirabilis, P. gingivalis, E. coli, and cephalosporins. 5-6 Pseudomonas aeruginosa, Shigella flexneri, Staphylococcus aureus, or Salmonella enterica. The Reagent antibody is recommended to be used in various Supplied as a solution in 0.01 M phosphate buffered immunological techniques, including immunoblot and saline, pH 7.4, containing 15 mM sodium azide as a ELISA. Detection of the P. vulgaris band by preservative. immunoblotting is specifically inhibited by the immunogen. Antibody Concentration: 1.0 mg/mL

Proteus vulgaris, a Gram-negative rod-shaped Precautions and Disclaimer bacterium, belongs to the Enterobacteriaceae family. For R&D use only. Not for drug, household, or other Members of the genus Proteus (Proteus spp.), which uses. Please consult the Safety Data Sheet for also includes Proteus mirabilis, Proteus penneri, and information regarding hazards and safe handling Proteus hauseri, originally characterize by their ability practices. to swarm on solid surfaces, are widespread in the environment and the gastrointestinal tract of human Storage/Stability and animals and known to be an opportunistic For continuous use, store at 2–8 C for up to one pathogens isolated from urine, wounds, and other month. For extended storage, freeze in working clinical sources. The Proteus spp. bacteria, are aliquots. Repeated freezing and thawing is not distinguished by their reactions for indole production, recommended. If slight turbidity occurs upon prolonged 1-3 salicin fermentation, and aesculin hydrolysis. storage, clarify the solution by centrifugation before P. vulgaris produces indole, which differentiates it from use. Working dilution samples should be discarded if 1-3 the indole-negative P. mirabilis and P. penneri. not used within 12 hours.

Proteus spp. bacteria may also be found in soil or water Product Profile habitats where they often regarded as indicators of Immunoblotting: a working of concentration fecal pollution and a contamination threat for potential 0.05-0.1 g/mL is recommended using dead 1 water or seafood poisoning. P. vulgaris bacteria lysate.

Note: In order to obtain best results in various techniques and preparations, it is recommended to determine optimal working dilutions by titration test.

References 1. Drzewiecka, D., Microb. Ecol., 72, 741-758 4. Ghaidaa, M., et al., N.Y. Sci. J., 6, 8-14 (2013). (2016). 5. Bahashwan, S.A., and Shafey, H.M., Euro. Sci. 2. O'Hara, C.M., et al., Int. J. Syst. Evol. Microbiol., J., 9, 188-202 (2013). 50, 1869-75 (2000). 6. Pal, N., et al., Ann. Med. Health Sci. Res., 6, 3. O'Hara, C.M., et al., Clin. Microbiol. Rev., 13, 267-73 (2016). 534-46 (2000). VS,DR,OKF,MAM 08/19-1

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