Radiocarbon Dates from the Grotte Du Renne and Saint-Césaire Support a Neandertal Origin for the Châtelperronian
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Radiocarbon dates from the Grotte du Renne and Saint-Césaire support a Neandertal origin for the Châtelperronian Jean-Jacques Hublina,1, Sahra Talamoa, Michèle Julienb, Francine Davidb, Nelly Connetb,c, Pierre Bodub, Bernard Vandermeerschd, and Michael P. Richardsa,e aDepartment of Human Evolution, Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany; bUnité Mixte de Recherche 7041 du Centre National de la Recherche Scientifique, Archéologies et Sciences de l’Antiquité, 92023 Nanterre, France; cInstitut national de recherches archéologiques préventives (INRAP), 86000 Poitiers, France; dUnité Mixte de Recherche 5199 du Centre National de la Recherche Scientifique—Préhistoire à l’Actuel: Culture, Environnement et Anthropologie, Université Bordeaux 1, 33405 Talence cedex, France; and eDepartment of Anthropology, University of British Columbia, Vancouver, BC, Canada V6T 1Z1 Edited by Richard G. Klein, Stanford University, Stanford, CA, and approved September 24, 2012 (received for review July 27, 2012) The transition from the Middle Paleolithic (MP) to Upper Paleo- Despite this fossil evidence, the question of whether Nean- lithic (UP) is marked by the replacement of late Neandertals by dertals manufactured the CP remains the topic of intense debate. modern humans in Europe between 50,000 and 40,000 y ago. This results in part from the fact that the CP is the so-called Châtelperronian (CP) artifact assemblages found in central France “transitional” assemblage that documents the broadest spectrum and northern Spain date to this time period. So far, it is the only of behavioral features reminiscent of the subsequent local UP, such assemblage type that has yielded Neandertal remains directly which was undisputedly created by modern humans. These fea- associated with UP style artifacts. CP assemblages also include tures include the production of bladelets (10), bone artifacts, and body ornaments, otherwise virtually unknown in the Neandertal body ornaments (11, 12), all of which are virtually unknown in world. However, it has been argued that instead of the CP being the Neandertal world. Furthermore, these body ornaments, often manufactured by Neandertals, site formation processes and layer considered as an expression of advanced symbolic behavior, have admixture resulted in the chance association of Neanderthal only been found in the CP of the Grotte du Renne and Quinçay, remains, CP assemblages, and body ornaments. Here, we report both at the northernmost limit of the CP geographical distribu- a series of accelerator mass spectrometry radiocarbon dates on tion (Fig. S1). ultrafiltered bone collagen extracted from 40 well-preserved bone When accepted, the association of these objects with Nean- fragments from the late Mousterian, CP, and Protoaurignacian dertals has been interpreted in two ways. For some (e.g., refs. 8 layers at the Grotte du Renne site (at Arcy-sur-Cure, France). Our and 13–15), this new behavior demonstrates a cultural impact on radiocarbon results are inconsistent with the admixture hypothe- the last Neandertals by contemporaneous UP modern human sis. Further, we report a direct date on the Neandertal CP skeleton populations, already present further east in Europe. For others from Saint-Césaire (France). This date corroborates the assignment (16), it represents a Neandertal invention of their own UP-style ANTHROPOLOGY of CP assemblages to the latest Neandertals of western Europe. industry independently of modern humans. Importantly, our results establish that the production of body A third group of authors speculates that the Neandertal/CP ornaments in the CP postdates the arrival of modern humans in association may in fact result from various site formation pro- neighboring regions of Europe. This new behavior could therefore cesses including layer admixture (17). For the Grotte du Renne have been the result of cultural diffusion from modern to Nean- specifically it has been suggested that the Neandertal remains dertal groups. might have been dug out of the underlying Mousterian layers by CP modern humans (17) and/or that CP body ornaments and stimulus diffusion | cultural modernity other bone artifacts may have moved down from the overlaying UP layers (18). At Saint-Césaire, the stratigraphic integrity of the rucial to understanding the replacement processes of Nean- CP deposits has also been challenged (17). Cdertals by modern humans in western Eurasia at the begin- Radiometric arguments are relevant to the assessment of ning of the Upper Paleolithic (UP) is the interpretation of so- possible mixing of younger or older intrusive material into the called “transitional industries.” In archeological sites, these lithic CP layers. The precise dating of the CP is also central to the assemblages are found under various types of early UP (mostly discussion surrounding its interpretation. In particular, the no- Aurignacian) and/or above genuine Middle Paleolithic (MP) tion that the development of the CP resulted from the cultural influence of modern populations implies that CP innovations assemblages. They display various proportions of UP artifacts postdate the arrival of modern humans in Europe. The radio- and technologies, but in several cases, a direct origin in the local metric dating of the CP at the Grotte du Renne and in other sites MP has been argued (1, 2). Although, in Europe, only Nean- has long been challenging because these assemblages lay at the dertal remains have been found in association with Mousterian limit of the application range of the 14C method. In particular, industries and only anatomically modern human remains have contamination problems have impaired most of the dates obtained been securely identified in Aurignacian layers, the biological identity of the makers of the transitional industries remains un- clear (3). Author contributions: J.-J.H. designed research; J.-J.H., S.T., M.J., F.D., N.C., and P.B. per- That Neandertals made some of the transitional industries has formed research; S.T., B.V., and M.P.R. contributed new reagents/analytic tools; J.-J.H., at times been suggested based on fragmentary paleontological S.T., M.J., F.D., N.C., P.B., and M.P.R. analyzed data; and J.-J.H. and S.T. wrote the paper. evidence (4, 5) or on indirect chronological arguments (6). The authors declare no conflict of interest. However, to date, only the Châtelperronian (CP), a transitional This article is a PNAS Direct Submission. assemblage from central and southwestern France, and northern Freely available online through the PNAS open access option. fi 1 Spain, has yielded well-identi ed and relatively abundant Ne- To whom correspondence should be addressed. E-mail: [email protected]. fi andertal remains, speci cally from two French sites (La Grotte This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10. du Renne and Saint-Césaire) (7–9). 1073/pnas.1212924109/-/DCSupplemental. www.pnas.org/cgi/doi/10.1073/pnas.1212924109 PNAS | November 13, 2012 | vol. 109 | no. 46 | 18743–18748 Downloaded by guest on September 25, 2021 before recent technical improvements in the pretreatment of targeted those areas that yielded CP body ornaments or diagnostic Nean- samples, which have increased the reliability of 14C dates beyond dertal remains (Fig. S5). Thirty-one samples are from the CP deposits, 4 are 40 ka 14CBP(19–21). from the underlying Mousterian and 5 are from the Protoaurignacian. In this context, a series of 31 accelerator mass spectrometry Our primary selection criterion was good bone preservation. Thus, we did (AMS) ultrafiltered dates on bones, antlers, bone artifacts, and not sample worked elements because they often have little well-preserved compact bone and may have been treated with unrecorded consolidants, teeth from key archaeological layers of the Grotte du Renne which can alter the radiocarbon age. We sampled a large number of thick, have been published (22). This study showed an unexpectedly untreated pieces of compact bone from shafts, mostly of reindeer and horse, high degree of intralayer variation in the radiometric ages and to assess possible movements of material between stratigraphic layers and to brought some support to the admixture hypothesis. compare their ages to those of worked artifacts published for the site (22). In contrast, a subsequent reinvestigation of the horizontal and Additionally, 35% of the selected fragments displayed anthropogenic marks vertical distribution of various categories of archaeologically di- (cut-marks or percussion marks). agnostic objects at the Grotte du Renne did not support the Extraction of collagen, isotopic measurements, and graphitization were hypothesis of large or small scale post depositional mixing. It performed at the Department of Human Evolution, Max Planck Institute for instead suggested that incomplete sample decontamination is the Evolutionary Anthropology, in Leipzig, Germany. All bone samples presented most parsimonious explanation for the observed radiometric here were subject to the following pretreatment procedures (19). Samples were first cleaned by sand blasting, and then 500 mg of bone powder was anomalies (11). In a response, the authors of the AMS dates did taken. The samples were decalcified in 0.5 M HCl at room temperature. not dispute this spatial evidence but also found no ground for the Later, 0.1 M NaOH was added to remove humics, and the treatment was claim of contamination by consolidants (23). The debate also completed with the addition of 0.5 M HCl. The resulting solid was gelati- focused on the definition of the stratigraphic units to be used in nized after Longin (30, 31) at pH 3 in a heater block at 75 °C. The gelatin was the Bayesian modeling of the radiocarbon dates (24, 25). filtered in an Eeze-Filter (Elkay Laboratory Products) to remove small (<8 μm) To test these alternative interpretations of the association at particles and then ultrafiltered with a Sartorius “Vivaspin 15” 30-kDa Grotte du Renne of Neandertals with the CP and UP type ultrafilter. Before use, the filter was cleaned to remove carbon containing artifacts, we produced a series of radiocarbon ages from the humectants.