Effect of Ischemia on Nucleosides and Bases in Rat Liver and Hepatoma 3924A1
[CANCER RESEARCH 47, 3092-3096, June 15. 1987] Effect of Ischemia on Nucleosides and Bases in Rat Liver and Hepatoma 3924A1 Konrad Pillwein, Hiremagalur N. Jayaram, and George Weber2 laboratory for Experimental Oncology, Indiana University School of Medicine, Indianapolis, IN 46223 ABSTRACT MATERIALS AND METHODS A high-pressure liquid Chromatographie method was developed which Materials. Nucleobases and nucleosides were obtained from Sigma achieved a separation and quantitation of 20 biologically important Chemical Co., St. Louis, MO. Ammonium acetate, ammonium phos nucleosides and bases. The concentrations of pyrimidine nucleosides and phate, sodium acetate, and acetonitrile (HPLC grade) were purchased bases, namely deoxycytidine, cytosine, cytidine, uracil, and uridine (22.6, from Fisher Scientific Co., Springfield, NJ. Radial Pak, Resolve Ct8 5 10.1, 5.2, 2.9, and 2.4 nmol/ml, respectively) were high in plasma, Mm(8.0 mm x 10 cm), Partisil 10-SAX (8.0 mm x 10 cm) columns, whereas purine nucleosides and bases were present in concentrations less and HPLC apparatus consisting of 720 System Controller, 730 Data than 2.5 nmol/ml. In erythrocytes, the pools of xanthine, hypoxanthine, Module, 71OB-WISP auto injector, 510 pumps, and 440 dual channel and xanthosine were 32-, 27-, and 22-fold larger, respectively, whereas absorbance detector were obtained from Waters Co., Milford, MA. cytidine, uridine, and deoxycytidine were only 21, 12, and 5% of plasma Animals and Tumors. Inbred male rats of the ACI/N strain weighing concentrations. The results suggest a compartmental system for transport 180-200 g (HarÃanIndustries, Cumberland, IN) were housed in indi of some of the purine and pyrimidine nucleosides and bases in the whole vidual cages and fed laboratory chow; water was available ad libitum.
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