Recombinant Human Activin a Receptor, Type 1B (ACVR1B)

Recombinant Human Activin A Receptor, Type 1B (ACVR1B)

Publication Part Number MAN0004539 Rev. 1.00

Catalog Number: PHC9284 PHC9285 PHC9281 PHC9283 Quantity: 10 μg 25 μg 100 μg 1 mg

Lot Number: See product label.

Molecular Weight: 38.2 kDa, predicted. Due to glycosylation ACVR1B appears ~46 kDa on SDS-PAGE run under reducing conditions.

Purity: ≥95% pure by SDS-PAGE

Amino Acid The protein sequence corresponds to the Extracellular region of mature Human ACVR1B, aa 24-126, fused to the Sequence: Fc region of Human IgG1. SGPRGVQALL CACTSCLQAN YTCETDGACM VSIFNLDGME HHVRTCIPKV ELVPAGKPFY CLSSEDLRNT HCCYTDYCNR IDLRVPSGHL KEPEHPSMWG PVEENLYFQG GSGTKLDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHGDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK

Biological Activity: In a functional ELISA, immobilized recombinant Human ACVR1B (0.2μg/mL) will bind recombinant Human Cripto-1 (Cat no.: PHC8014, PHC8015, PHC8011, PHC8013) with a linear range of 0.8–100 ng/mL.

Biological Function: ACVR1B or ALK-4 is a type I transmembrane serine/threonine receptor kinase consisting of a 103 aa extracellular domain, a 23 aa transmembrane domain and a 356 aa cytoplasmic domain. ACVR1B acts as a transducer of Activin or activin like ligand signals. Activin binds to either type II receptor ACVR2A or ACVR2B which then forms a stable complex with ACVR1B. In the process ACVR1B is phosphorylated within its cytoplasmic domain by the type II receptor and the signal transduction cascade is initiated. ACVR1B also transduces signals of nodal, GDF-1, and Vg1; however, unlike activin, they require other coreceptor molecules such as the protein Cripto.

Formulation: Lyophilized, carrier-free.

Sterility: Filtered prior to lyophilization through a 0.22 micron sterile filter.

Endotoxin: <0.1 ng/μg

Production: Recombinant Human ACVR1B is expressed in Human Embryonic Kidney 293 cells and purified via sequential chromatography.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Recommendation: Reconstitute lyophilized Human ACVR1B in sterile, distilled water to a concentration of 0.1–0.5 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ –20°C. Further dilution should be made in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA.

Suggested The optimal concentration should be determined for each specific application. Working Dilutions:

Storage: Lyophilized Human ACVR1B should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted Human ACVR1B at ≤ –20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.

Expiration Date: Expires one year from date of receipt when stored as instructed. References: Harrison, C.A., P.C. Gray, S.C. Koerber, W. Fischer, W. Vale (2003). Identification of a functional binding site for activin on the type I receptor ALK4. J. Biol. Chem. 278(23): 21129–21135. Bianco, C., H.B. Adkins, C. Wechselberger, et al. (2002). Cripto-1 activates nodal- and ALK4-dependent and - independent signaling pathways in mammary epithelial Cells. Mol. Cell. Biol. 22(8): 2586–2597. Explanation of Symbols The symbols present on the product label are explained below:

Symbol Description Symbol Description Catalog Number Batch code

Research Use Only In vitro diagnostic medical device

Use by Temperature limitation

Manufacturer European Community authorized representative

Without, does not contain With, contains

Protect from light Consult accompanying documents

Directs the user to consult instructions for use (IFU),

accompanying the product.

Limited Use Label License: Research Use Only The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product only to perform internal research for the sole benefit of the purchaser. No right to resell this product or any of its components is conveyed expressly, by implication, or by estoppel. This product is for internal research purposes only and is not for use in commercial applications of any kind, including, without limitation, quality control and commercial services such as reporting the results of purchaser’s activities for a fee or other form of consideration. For information on obtaining additional rights, please contact [email protected] or Out Licensing, Life Technologies, 5791 Van Allen Way, Carlsbad, California 92008.

For Research Use Only. Caution: Not for human or animal therapeutic or diagnostic use. Manufactured under ISO 13485 Quality Standard Manufacturing site: 7335 Executive Way | Frederick, MD 21704 | Toll Free in USA 800.955.6288 .

For support visit www.lifetechnologies.com/support or email [email protected] www.lifetechnologies.com Revision Date 16 June 2011