US 2015O140141A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2015/0140141 A1 Milow et al. (43) Pub. Date: May 21, 2015 (54) METHODS FOR BOTANCAL AND/OR Publication Classification ALGAE EXTRACTION (51) Int. Cl. (71) Applicants: BASF CORPORATION, FLORHAM A6R8/97 (2006.01) PARK, NJ (US); BASF BEAUTY A61O 19/00 (2006.01) CARE SOLUTIONS FRANCE SAS, A61O I/06 (2006.01) Lyon (FR) A61O I/14 (2006.01) A61O 19/10 (2006.01) (72) Inventors: Clifford A. Milow, Massapequa, NY A618/49 (2006.01) (US); Laurent Bailly, Essey Les Nancy A61O 1704 (2006.01) (FR); Christopher Judd, Riverhead, NY (52) U.S. Cl. (US); Nicole Jeannine Richter, Port CPC. A61K 8/97 (2013.01); A61K 8/498 (2013.01); Jefferson, NY (US) A61O 19/00 (2013.01); A61O 1704 (2013.01); (73) Assignees: BASF BEAUTY CARE SOLUTIONS A61O 1/14 (2013.01); A61O 19/004 (2013.01); FRANCE SAS, Lyon (FR); BASF A61O 19/10 (2013.01); A61O 19/005 CORPORATION, FLORHAMPARK, (2013.01); A61O I/06 (2013.01) NJ (US) (21) Appl. No.: 14/402.719 (57) ABSTRACT (22) PCT Fled: Jun. 6, 2013 The present disclosure relates to methods for extraction of biomass. Biomass of most interest is that which contains (86) PCT NO.: PCT/US2O13/044464 biologically active, extracts suitable for the skin care market. S371 (c)(1), The biomass of interest includes botanicals (plant extracts (2) Date: Nov. 21, 2014 and bioferments thereof), algae (red, brown, green and red, including bioferments thereof), fungi and even animal Related U.S. Application Data extracts (insect, crustacean) origin. Further the use of said (60) Provisional application No. 61/656.221, filed on Jun. extracts in cosmetic preparations prepared by the disclosed 6, 2012. method is envisioned. Patent Application Publication May 21, 2015 Sheet 1 of 2 US 2015/O140141 A1 000’0 W Patent Application Publication May 21, 2015 Sheet 2 of 2 US 2015/O140141 A1 5% FuCuS VesiculoSuS Extraction in Water On Left And Extraction 10% Pluronic F127/Water Solution On The Right. FIG.2 US 2015/O 140141 A1 May 21, 2015 METHODS FOR BOTANCAL AND/OR 0009 B. Description of Related Art ALGAE EXTRACTION 00.10 Extracts may be obtained from the whole plant, algae or fungi (i.e., the entire plant is used to prepare the 0001. This application takes the benefit of U.S. Provi extract) or from a part of the plant (e.g., leaf, stem, root, sional Application No. 61/656.221 filed Jun. 6, 2012, the flower, seed, sap, bark, etc.). The extracts can also be derived contents herein incorporated entirely by reference. by fermentation which helps to breakdown the cellular tissue of the algae or plant, thereby possibly increasing the bioavail BACKGROUND OF THE INVENTION ability of the naturally occurring bioactive targets. 0011. During extraction typically more than one com 0002 A. Field of the Invention pound is extracted from the botanical or algae and will fre quently range from at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 0003. The present invention relates to methods for extrac 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, tion of biomass. Biomass of most interest is that which con 30, 31, 32, 33, 34, or 35 of differing extractants in a single tains biologically active extracts suitable for the skin treat composition. ment market. The biomass of interest includes botanicals 0012. The skin active targets of particular interest are the (plants and bioferments thereof), algae (red, brown, green and flavonoids and flavonoid derivatives. red, including bioferments thereof), fungi and even animal 0013 Typically the plant (or any part of the plant such as (insect, crustacean) origin and the use of said extracts in the leaves, stems, bark, roots, fruit, flowers or flower buds, cosmetic preparations prepared by the disclosed method. seeds, seed pods, sap, whole plant, etc.) is disrupted by 0004. These natural product hydrophobic extracts are mechanical means which results in a puree. The puree is then often used in cosmetic and pharmaceutical application. Natu processed to be substantially free of impurities or undesired ral products, particularly botanically, fungi and algae derived solids. The puree can then be poured into a shallow vessel and such as flavonoids or flavonoid derivatives, have demon quickly exposed to low temperature, i.e., flash frozen, for strable beneficial properties on the skin and hair. For example, example at -20 degrees centigrade or lower, preferably under these extracts have demonstrated antimicrobial, antiseptic, a vacuum for removal of water content (lyophilization). anti-inflammatory, antioxidant, enzyme stimulation or inhi 0014 Aqueous, alcoholic, or oil based extraction tech bition, pigmentation enhancement or control, photoprotec niques, or combinations thereof, have also been used in the tive, treatment of skin aging, skin imperfections, dry skin, past and are used on the whole plant or any part thereof of photodamaged skin, wrinkles, age spots, acne, skin lighten (e.g., leaves, stems, bark, roots, fruit, flowers or flower buds, ing, psoriasis, and atopic dermatosis. seeds, seedpods, sap, whole plant, etc.) to produce an extract. 0005. The botanical, algae and fungal species are for In such a process, the desired part of the plant or the whole example any botanical, algae or fungal species which may plant is crushed up (e.g., blender) and then extracted with the server as a source for flavonoid or flavonoid derivatives. desired solvent (e.g., water, alcohol, waterfalcohol, or oil 0006 More particularly for example the biomass may be based solvents) to obtain the desired extract. The extract can selected from but is not limited to the group consisting of then be stored in liquid form, lyophilized, or subject to further Acacia Senegal, Achillea millefolium, Aloe barbadensis, Ana processing techniques (e.g., heating, cooling, etc.). Extrac nus sativus, Argania spinosa, Avena sativa, Cassia alata, tion processes are well-known to those having ordinary skill Cocoa Callus, Cocos nucifera, Bupleurum falcatum, Buty in the extract field (e.g., maceration, infusion, percolation, rospermum parkii, Calluna vulgaris, Camelia sinensis, digestion, decoction, hot continuous extraction, aqueous-al Chondrus crispus, Centella asiatica, Ceratonia siliqua, Ces coholic extract, counter current extract, microwave assisted trum latifolium, Cinnamomum cassia, Citrus limon, Coffea extraction, ultrasound extraction, Supercritical fluid extracts, Arabica, Cola acuminate, Cucumissativus, Durio zibethinus, phytonic extract (e.g., with hydro-fluoro-carbon solvents), Glycine soia (Soybean), Glycyrrhiza glabra, Gymnema etc. Sylvestre, Haslea Ostrearia, Heliantus annuus, Hibiscus abel 0015. It is known that certain surfactant water based sys moschus, Humulus lupulus, Laminaria digitata, Lepidium tems may be used for extraction of various hydrocarbons. For meyenii, Linum usitatissimum, Macadamia termifolia, Malva example, Calvert, T. L. et al. AICHE Journal, (1994), Vol. 40. Sylvestris, Melissa officinalis, Morus alba, Morus bombycis, #9, p 1449-1457: Hurter, P. N. et al. Langmuir 1992, 8, Nereocystis luetkeana, Olea europaea, Orthosiphon sta 1291-1299 teach the extraction of hydrocarbons from water mineus, Palmeria palmate, Peucedanum graveolens, Peumus waste systems. boldus, Pisum sativum, Pueraria lobata, Punicia granatum, 0016. Additionally, it is also known that some ethanol Pyrus malus, Rheum palmatum, Rhodiola crenulata, Ros derived plant extracts can be solubilized in aqueous solutions marinus officinalis, Saxifraga sarmentosa, Sarrcodiotheca containing poly(ethylene-oxide)-poly(propylene oxide) gaudichaudii, Scutellaria baicalensis, Serenoa serrulata, copolymers (V Dinoiu. et al. Revista de Chimie, 62/4, 396 Spirulina platensis, Theobroma cacao, Tuber magnatum, 400). 0017. Further, U.S. Serial No. 2012/0010390 discloses the Uncaria tomentosa, Vitis vinifera, Ptychopetalum Olacoides, use of aqueous two phase systems for isolation of biomol Zea mays and Zingiber officinale. ecules or target compounds such as an antibody or a protein 0007. In particular extracts from algae are of special inter from a fermentation broth using ethylene oxide and propy est. For example, extraction of Chondrus crispus, Nereocystic lene oxide random copolymers dissolved in one of the liquid luetkeana and Sarrcodiotheca gaudichaudii, Fucus vesiculo phases. PCT application No. WO2012011589 discloses a sus, Sarcodiotheca gaudichaudii, Ulva lactuca, Laminaria method for the manufacturing of fat-soluble bioactive sub longicruris, Nannochloropsis oculata, Tetraselmis suecica stances extracted from microbial cells using an organic Sol are of special interest. vent in combination with a Surfactant. 0008 Extracts from plants such as Cassia alata, Argania 0018. There are however numerous disadvantages to spinosa and Cocoa Callus are also of preferred interest. extracting with solvents or even water especially when the US 2015/O 140141 A1 May 21, 2015 extracts of interest are lipophilic, hydrophobic or ing liquid. It would be highly desirable to improve this filter amphiphilic. Although water is environmentally friendly, it is ing step by speeding up the filtering process and the elimina limited as an effective extracting solvent when the desired tion of multiple filters. extractants are hydrophobic. Organic solvents are less desir able because of their possible flammability and environmen BRIEF DESCRIPTION OF THE DRAWINGS tal drawbacks. Furthermore, when the extractants are desired for use in cosmetics, in particular skin active cosmetics Such 0024 FIG. 1 is a comparison of extraction fingerprints as moisturizes etc., solvents are most undesirable because of from Nereocystis luetkeana using HPLC and different extrac their irritating or drying effects on skin. The organic Solvent tion mediums. can usually be removed by evaporation but this is an addi 0025 Bottom: 5% algae extraction medium with butylene tional step with related economic and environmental costs. glycol 0019. Further there is a need to retrieve more complex 0026. Fourth: 5% algae extraction medium with DI water. targeted biomass extracts which can be obtained through (0027. Third: 5% algae extraction medium with 10 wt.% traditional aqueous, glycol or ethanol extractions and prefer Pluronic(R) L44 in water.