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Materiały Konferencyjne Collegium Medicum Uj – Abstrakty 7

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Materiały Konferencyjne Collegium Medicum Uj – Abstrakty 7 ZESZYTY NAUKOWE TOWARZYSTWA DOKTORANTÓW UNIWERSYTETU JAGIELLOŃSKIEGO RADA NAUKOWA „ZESZYTÓW NAUKOWYCH TOWARZYSTWA DOKTORANTÓW UJ” Przewodniczący Rady Naukowej Prof. dr hab. Wojciech Nowak | Rektor Uniwersytetu Jagiellońskiego Dr Denys Azarov | Uniwersytet Narodowy „Akademia Kijowsko-Mohylańska” Prof. Martin Bier | East California University Prof. dr hab. Andriy Boyko | Lwowski Uniwersytet Narodowy im. Iwana Franki Prof. Hugh J. Byrne | FOCAS Research Institute, Dublin Institute of Technology Dr hab. Adrián Fábián | University of Pécs Prof. dr hab. Maria Flis | Uniwersytet Jagielloński Prof. dr hab. Tadeusz Gadacz | Uniwersytet Pedagogiczny w Krakowie Dr Herbert Jacobson | Linköping Universitet Prof. dr hab. Katarzyna Kieć-Kononowicz | Uniwersytet Jagielloński Dr Miklós Kiss | University of Groningen Dr Erdenhuluu Khohchahar | Kyoto University Prof. dr hab. Andrzej Kotarba | Uniwersytet Jagielloński Dr Oleksiy Kresin | Narodowa Akademia Nauk Ukrainy Prof. dr hab. Marta Kudelska | Uniwersytet Jagielloński Prof. dr hab. Tomasz Mach | Uniwersytet Jagielloński Prof. dr hab. Andrzej Mania | Uniwersytet Jagielloński Dr Kristin McGee | University of Groningen Prof. dr hab. Karol Musioł | Uniwersytet Jagielloński Prof. Biderakere E. Rangaswamy | Bapuji Institute of Engineering and Technology Dr Melanie Schiller | University of Groningen Prof. dr hab. Jacek Składzień | Uniwersytet Jagielloński Prof. dr hab. Leszek Sosnowski | Uniwersytet Jagielloński Prof. dr hab. Bogdan Szlachta | Uniwersytet Jagielloński Prof. Luigia di Terlizzi | Università degli Studi di Bari Aldo Moro Prof. Matthias Theodor Vogt | Institut für kulturelle Infrastruktur Sachsen ZESZYTY NAUKOWE TOWARZYSTWA DOKTORANTÓW UNIWERSYTETU JAGIELLOŃSKIEGO ____________________________________________________ NAUKI ŚCISŁE ~ NUMER 14 (1/2017) ~ VII KONFERENCJA DOKTORANTÓW UNIWERSYTETU JAGIELLOŃSKIEGO – COLLEGIUM MEDICUM 27 MAJA 2017 ROKU, KRAKÓW KRAKÓW 2017 Zeszyty Naukowe Towarzystwa Doktorantów UJ ul. Czapskich 4/14, 31-110 Kraków www.doktoranci.uj.edu.pl/zeszyty Redaktor naczelny: Arkadiusz Nyzio Zastępczyni redaktora naczelnego: Ewa Modzelewska Sekretarz redakcji: Rafał Kur Redaktor prowadzący serii: Wojciech Tomczyk Redaktorzy tomu: Bartłomiej Jałocha i Arkadiusz Nyzio Komitet Naukowy VII Konferencji Doktorantów Collegium Medicum UJ: prof. dr hab. Tomasz Mach (kierownik studiów doktoranckich, Wydział Lekarski) prof. dr hab. Jolanta Jaworek (kierownik studiów doktoranckich, Wydział Nauk o Zdrowiu) dr hab. Kinga Sałat, prof. UJ (kierownik studiów doktoranckich, Wydział Farmaceutyczny) dr hab. Krzysztof Gil (kierownik „International PhD studies in medical sciences”) prof. dr hab. Grażyna Bochenek prof. dr hab. Barbara Filipek prof. dr hab. Rafał Olszanecki dr hab. Jan Bilski, prof. UJ dr hab. Marek Kuźniewski, prof. UJ dr hab. Jerzy Tomik, prof. UJ dr hab. Marta Cześnikiewicz-Guzik dr hab. Krzysztof Kamiński dr hab. Danuta Owczarek Numer czasopisma finansowany ze środków Towarzystwa Doktorantów UJ oraz Collegium Medicum UJ Współpraca wydawnicza: WYDAWNICTWO n o w a s t r o n a www.wydawnictwonowastrona.pl e-mail: [email protected] ___________________________________________________________________________ © Copyright by Zeszyty Naukowe Towarzystwa Doktorantów UJ All rights reserved Wydanie I, Kraków 2017 Nakład: 200 egz. e-ISSN 2082-3827 p-ISSN 2084-977X Spis treści MATERIAŁY KONFERENCYJNE COLLEGIUM MEDICUM UJ – ABSTRAKTY 7 MARTA MAŚLANKA 173 Substytucja octanów allilowych nukleofilami fosforowymi oraz zawierającymi atom fosforu ANNA LASKA 187 Biomateriały stosowane w inżynierii tkankowej do regeneracji tkanek INFORMACJE O AUTORACH 197 Contents CONFERENCE MATERIALS – ABSTRACTS 7 MARTA MAŚLANKA 173 Substitution of Allyl Octanoates with Phosphorus Nucleophiles and with Phosphorus-Containing Compounds ANNA LASKA 187 Biomaterials Used in Tissue Engineering for Tissue Regeneration ABOUT THE CONTRIBUTORS 197 ZESZYTY NAUKOWE TOWARZYSTWA DOKTORANTÓW UJ 7 _____________________________________________________________________________________________________ MICHAŁ ABRAM*, ANNA RAPACZ**, KRZYSZTOF KAMIŃSKI* * ZAKŁAD CHEMII LEKÓW, KATEDRA CHEMII FARMACEUTYCZNEJ, WYDZIAŁ FARMACEUTYCZNY, COLLEGIUM MEDICUM, UNIWERSYTET JAGIELLOŃSKI; ** ZAKŁAD FARMAKODYNAMIKI, KATEDRA FARMAKODYNAMIKI, WYDZIAŁ FARMACEUTYCZNY, COLLEGIUM MEDICUM, UNIWERSYTET JAGIELLOŃSKI _______________________________________________________________________________________ Związki hybrydowe, pochodne kwasu (2,5-dioksopirolidyn-1-ylo)(fenylo)octowego o szerokim działaniu ochronnym w zwierzęcych modelach padaczki Padaczka, na którą cierpi ponad 50 mln ludzi na świecie, należy do najczęst- szych chorób neurologicznych. Ze względu na swoją złożoność i niepoznany do końca patomechanizm, jak również często występującą lekooporność, jest chorobą trudną do farmakoterapii, a dodatkowo obecnie stosowane leki przeciwpadaczkowe obarczone są wieloma, często poważnymi, działaniami niepożądanymi. Dlatego też istnieje duże zapotrzebowanie na nowe leki prze- ciwpadaczkowe, skuteczne w różnych typach padaczki, w tym w napadach lekoopornych. W ostatnim czasie poszukiwania nowych substancji leczniczych ukierunko- wane są na związki wielofunkcyjne, które oddziałując jednocześnie z wie- loma celami biologicznymi, mogą wykazywać synergiczny i komplekso- wy efekt leczniczy. Teoria ta znalazła zastosowanie i została potwierdzona w grupie hybrydowych substancji przeciwdrgawkowych, będących amido- wymi pochodnymi kwasów 2-(2,5-dioksopirolidyn-1-ylo)-alkilowych [1, 2]. Związki te charakteryzowały się szerokim spektrum aktywności w teście maksymalnego elektroszoku (MES), teście drgawek wywołanych podskór- nym podaniem pentetrazolu (scPTZ) oraz w drgawkach sześciohercowych (6-Hz), które uważane są za najważniejsze testy przedkliniczne, identyfiku- jące kandydatów do stosowania nowych, skutecznych leków przeciwpadacz- kowych. Prezentowane badania stanowią kontynuację prac chemiczno-farmakolo- gicznych w grupie hybrydowych substancji przeciwdrgawkowych pochod- nych pirolidyno-2,5-dionu. W efekcie zaprojektowano i w wyniku kilkueta- powego postępowania syntetycznego otrzymano serię analogów opisanych wcześniej hybryd, charakteryzujących się obecnością w strukturze che- micznej dodatkowego pierścienia aromatycznego. Wyniki badań farmako- logicznych in vivo pokazały, iż tego typu modyfikacja spowodowała istotne zwiększenie aktywności we wszystkich wymienionych powyżej modelach drgawek padaczkowych. Udowodniono też wpływ stereochemii związków na efekt farmakologiczny. 8 ZESZYTY NAUKOWE TOWARZYSTWA DOKTORANTÓW UJ ______________________________________________________________________________________________________ Projekt został sfinansowany ze środków Narodowego Centrum Nauki nr 2015/ 18/E/NZ7/00509. Bibliografia: 1. Kamiński K. et al., “J. Med. Chem.” 2015, 23, s. 2548–2561. 2. Kamiński K. et al., “J. Bioorg. Med. Chem.” 2016, 24, s. 606–618. MATERIAŁY KONFERENCYJNE COLLEGIUM MEDICUM UJ – ABSTRAKTY 9 _____________________________________________________________________________________________________ VERONIKA ALEKSANDROVYCH*, MICHAŁ WOLNICKI**, KRZYSZTOF GIL* * DEPARTMENT OF PATHOPHYSIOLOGY, JAGIELLONIAN UNIVERSITY, MEDICAL COLLEGE; ** DEPARTMENT OF PEDIATRIC UROLOGY, JAGIELLONIAN UNIVERSITY, MEDICAL COLLEGE _______________________________________________________________________________________ The Role of Interstitial Cajal-Like Cells in the Ureteropelvic Junction in the Development of Congenital Megaureter in Children Introduction: Interstitial cells of Cajal play an important role in the regula- tion of gut motility. Similar cells, called interstitial Cajal-like cells (ICLC), are present in the urinary tract. We assume that ICLC play an important role in propagation and regulation of ureteral peristalsis, thus changes in the ICLC density in ureteral wall might be responsible for the development of mega- ureter and hydronephrosis. Objectives: The aim of this study was to evaluate the location of ICLC in the renal pelvis and ureteral wall using histopathological methods in children with obstructive and refluxing megaureter. Methods: The data from (15 and 10 patients, respectively) were compared. Samples of renal pelvis and proximal ureter were subjected to immunofluo- rescence staining to detect ICLC (CD117) and interstitial plexus neurons (PGP 9.5). Toluidine blue staining for mast cells detection and Masson’s trichrome staining – for collagen deposits were used additionally. Results: The c-Kit receptor (CD117) positive cells were detected in renal pel- vis and ureter. Spindle interstitial cells (ICLC) were present in the tunica muscularis, along neurovascular septa, in between smooth muscle bundles and in urothelium. The density of ICLC and proportion of smooth muscle were lower in the refluxing megaureter. The number of mast cells was in- creased in the obstructive megaureter. There were no differences in the neu- ron count between groups examined. Conclusions: The differences in the histopathological aspects provide in- formation on the possible pathophysiology of obstructive and refluxing megaureters. Ureteral peristalsis can be affected by the decreased number of interstitial Cajal-like cells in refluxing megaureter. 10 ZESZYTY NAUKOWE TOWARZYSTWA DOKTORANTÓW UJ ______________________________________________________________________________________________________ ALEKSANDRA ARCISZEWSKA, MARCIN SIWEK, DOMINIKA DUDEK ZAKŁAD ZABURZEŃ AFEKTYWNYCH, KATEDRA PSYCHIATRII, WYDZIAŁ LEKARSKI, COLLEGIUM MEDICUM, UNIWERSYTET JAGIELLOŃSKI _______________________________________________________________________________________
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