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Figure S1. Endogenous MIR45

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Figure S1. Endogenous MIR45 Figure S1. Endogenous MIR452 and VEGFA expression in CRC tissues and cell lines. (A) The expression of MIR452 was validated using 10 CRC tissue samples and matched normal colon tissue samples. miRNA levels were normalized to colon-specific RNU48. Values are presented as the fold- change in tumor tissue relative levels (ΔΔCT) to normal tissue. (B) The relative endogenous MIR452 expression levels in six CRC cell lines. The data are presented as a fold change in HT29, Caco2, HCT116, LoVo, and SW48 cells relative to SW480 cells. This experiment was performed as two independent experiments, each carried out in triplicate. (C) MIR452 expression level analysis by qRT- PCR for MIR452 transfection efficiency in Caco2 and SW48 cells. (D) The relative endogenous VEGFA expression levels in five CRC cell lines. The data are presented as a fold change in HT29, Caco2, HCT116, or SW48 cells relative to SW480 cells. This experiment was performed three independent experiments, each carried out in duplicate. Table S1. The putative target genes of MIR452 identified and predicted by the microarray analysis from the MIR452 overexpressed cells. Symbol Definition Accession Homo sapiens acyl-CoA thioesterase 8 (ACOT8), transcript ACOT8 NM_005469.2 variant 1, mRNA. Homo sapiens ARP6 actin-related protein 6 homolog (yeast) ACTR6 NM_022496.3 (ACTR6), mRNA. ADI1 Homo sapiens acireductone dioxygenase 1 (ADI1), mRNA. NM_018269.1 Homo sapiens aftiphilin (AFTPH), transcript variant 1, AFTPH NM_203437.2 mRNA. AHNAK2 Homo sapiens AHNAK nucleoprotein 2 (AHNAK2), mRNA. NM_138420.2 Homo sapiens A kinase (PRKA) anchor protein 7 (AKAP7), AKAP7 NM_004842.2 transcript variant alpha, mRNA. Homo sapiens anaphase promoting complex subunit 13 ANAPC13 NM_015391.2 (ANAPC13), mRNA. Homo sapiens ankyrin repeat domain 46 (ANKRD46), ANKRD46 NM_198401.2 mRNA. ANXA4 Homo sapiens annexin A4 (ANXA4), mRNA. NM_001153.2 ARF4 Homo sapiens ADP-ribosylation factor 4 (ARF4), mRNA. NM_001660.2 Homo sapiens arginine and glutamate rich 1 (ARGLU1), ARGLU1 NM_018011.3 mRNA. Homo sapiens Rho guanine nucleotide exchange factor ARHGEF11 NM_014784.2 (GEF) 11 (ARHGEF11), transcript variant 1, mRNA. Homo sapiens ADP-ribosylation factor-like 6 interacting ARL6IP1 NM_015161.1 protein 1 (ARL6IP1), mRNA. Homo sapiens ankyrin repeat and SOCS box-containing 8 ASB8 NM_024095.3 (ASB8), mRNA. ATMIN Homo sapiens ATM interactor (ATMIN), mRNA. NM_015251.2 Homo sapiens ATPase, Ca++ transporting, plasma NM_00100132 ATP2B1 membrane 1 (ATP2B1), transcript variant 1, mRNA. 3.1 Homo sapiens ATPase, H+ transporting, lysosomal V0 ATP6V0A2 NM_012463.2 subunit a2 (ATP6V0A2), mRNA. Homo sapiens UDP-Gal:betaGlcNAc beta 1,4- B4GALT6 NM_004775.2 galactosyltransferase, polypeptide 6 (B4GALT6), mRNA. Homo sapiens breast carcinoma amplified sequence 2 BCAS2 NM_005872.2 (BCAS2), mRNA. Homo sapiens BCL2-associated transcription factor 1 BCLAF1 NM_014739.2 (BCLAF1), transcript variant 1, mRNA. Homo sapiens biogenesis of lysosome-related organelles BLOC1S1 NM_001487.1 complex-1, subunit 1 (BLOC1S1), mRNA. Homo sapiens BCL2/adenovirus E1B 19kDa interacting BNIP3L NM_004331.2 protein 3-like (BNIP3L), mRNA. Homo sapiens breast cancer metastasis-suppressor 1-like BRMS1L NM_032352.3 (BRMS1L), mRNA. Homo sapiens basic transcription factor 3-like 4 (BTF3L4), BTF3L4 NM_152265.2 mRNA. Homo sapiens butyrophilin, subfamily 3, member A2 BTN3A2 NM_007047.3 (BTN3A2), mRNA. Homo sapiens basic leucine zipper and W2 domains 1 BZW1 NM_014670.2 (BZW1), mRNA. XM_943165 Homo sapiens chromosome 12 open reading frame 48 C12orf48 NM_017915.2 (C12orf48), mRNA. Homo sapiens chromosome 14 open reading frame 167 C14orf167 NR_023921.1 (C14orf167), transcript variant 1, non-coding RNA. Homo sapiens chromosome 1 open reading frame 56 C1orf56 NM_017860.3 (C1orf56), mRNA. Homo sapiens chromosome 3 open reading frame 58 C3orf58 NM_173552.2 (C3orf58), mRNA. Homo sapiens chromosome 4 open reading frame 34 C4orf34 NM_174921.1 (C4orf34), mRNA. Homo sapiens chromosome 6 open reading frame 62 C6orf62 NM_030939.3 (C6orf62), mRNA. CA9 Homo sapiens carbonic anhydrase IX (CA9), mRNA. NM_001216.1 Homo sapiens calmodulin regulated spectrin-associated CAMSAP1L1 NM_203459.1 protein 1-like 1 (CAMSAP1L1), mRNA. CASD1 Homo sapiens CAS1 domain containing 1 (CASD1), mRNA. NM_022900.3 Homo sapiens core-binding factor, beta subunit (CBFB), CBFB NM_001755.2 transcript variant 2, mRNA. Homo sapiens coiled-coil domain containing 61 (CCDC61), NM_00108040 CCDC61 mRNA. 2.1 Homo sapiens coiled-coil domain containing 90B CCDC90B NM_021825.3 (CCDC90B), mRNA. CCNG2 Homo sapiens cyclin G2 (CCNG2), mRNA. NM_004354.1 CD164 Homo sapiens CD164 molecule, sialomucin (CD164), mRNA. NM_006016.3 Homo sapiens cell division cycle 40 homolog (S. cerevisiae) CDC40 NM_015891.2 (CDC40), mRNA. Homo sapiens cadherin 13, H-cadherin (heart) (CDH13), CDH13 NM_001257.3 mRNA. Homo sapiens cyclin-dependent kinase 5, regulatory subunit CDK5R1 NM_003885.2 1 (p35) (CDK5R1), mRNA. CEP57 Homo sapiens centrosomal protein 57kDa (CEP57), mRNA. NM_014679.3 Homo sapiens claudin domain containing 1 (CLDND1), NM_00104018 CLDND1 transcript variant 1, mRNA. 1.1 Homo sapiens CDC-like kinase 1 (CLK1), transcript variant NM_00102464 CLK1 2, mRNA. 6.1 CLK4 Homo sapiens CDC-like kinase 4 (CLK4), mRNA. NM_020666.2 Homo sapiens CCHC-type zinc finger, nucleic acid binding CNBP NM_003418.1 protein (CNBP), mRNA. Homo sapiens coatomer protein complex, subunit gamma 2 COPG2 NM_012133.2 (COPG2), mRNA. Homo sapiens coenzyme Q10 homolog B (S. cerevisiae) COQ10B NM_025147.3 (COQ10B), mRNA. Homo sapiens cisplatin resistance-associated overexpressed CROP NM_016424.3 protein (CROP), transcript variant 1, mRNA. Homo sapiens CWC22 spliceosome-associated protein CWC22 NM_020943.2 homolog (S. cerevisiae) (CWC22), mRNA. Homo sapiens DCN1, defective in cullin neddylation 1, DCUN1D1 NM_020640.2 domain containing 1 (S. cerevisiae) (DCUN1D1), mRNA. Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 DDX17 NM_030881.2 (DDX17), transcript variant 2, mRNA. Homo sapiens Der1-like domain family, member 1 (DERL1), DERL1 NM_024295.3 mRNA. Homo sapiens dickkopf homolog 3 (Xenopus laevis) (DKK3), DKK3 NM_015881.5 transcript variant 1, mRNA. Homo sapiens deleted in lymphocytic leukemia 1 (non- DLEU1 NR_002605.1 protein coding) (DLEU1), non-coding RNA. Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member DNAJC10 NM_018981.1 10 (DNAJC10), mRNA. Homo sapiens dolichyl-phosphate mannosyltransferase DPM1 NM_003859.1 polypeptide 1, catalytic subunit (DPM1), mRNA. Homo sapiens dihydropyrimidinase-like 2 (DPYSL2), DPYSL2 NM_001386.4 mRNA. Homo sapiens down-regulator of transcription 1, TBP- DR1 NM_001938.2 binding (negative cofactor 2) (DR1), mRNA. Homo sapiens ELOVL family member 5, elongation of long ELOVL5 chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast) NM_021814.3 (ELOVL5), mRNA. Homo sapiens ectonucleoside triphosphate ENTPD5 NM_001249.1 diphosphohydrolase 5 (ENTPD5), mRNA. Homo sapiens epidermal growth factor receptor pathway EPS8 NM_004447.4 substrate 8 (EPS8), mRNA. Homo sapiens ERBB receptor feedback inhibitor 1 (ERRFI1), ERRFI1 NM_018948.2 mRNA. F2 Homo sapiens coagulation factor II (thrombin) (F2), mRNA. NM_000506.3 PREDICTED: Homo sapiens family with sequence similarity XM_001132771 FAM116A 116, member A (FAM116A), mRNA. .1 Homo sapiens family with sequence similarity 134, member NM_00103485 FAM134B B (FAM134B), transcript variant 1, mRNA. 0.1 Homo sapiens family with sequence similarity 150, member NM_00100291 FAM150B B (FAM150B), mRNA. 9.2 Homo sapiens family with sequence similarity 162, member FAM162A NM_014367.3 A (FAM162A), mRNA. Homo sapiens family with sequence similarity 8, member A1 FAM8A1 NM_016255.1 (FAM8A1), mRNA. Homo sapiens Fanconi anemia, complementation group I FANCI NM_018193.2 (FANCI), transcript variant 2, mRNA. Homo sapiens F-box and WD-40 domain protein 5 (FBXW5), FBXW5 NM_178226.1 transcript variant 3, mRNA. Homo sapiens fibronectin type III domain containing 3A NM_00107967 FNDC3A (FNDC3A), transcript variant 1, mRNA. 3.1 FTH1 Homo sapiens ferritin, heavy polypeptide 1 (FTH1), mRNA. NM_002032.2 Homo sapiens ferritin, heavy polypeptide-like 8 (FTHL8) on FTHL8 NR_002203.1 chromosome X. Homo sapiens GA binding protein transcription factor, alpha GABPA NM_002040.2 subunit 60kDa (GABPA), mRNA. Homo sapiens galactosamine (N-acetyl)-6-sulfate sulfatase GALNS (Morquio syndrome, mucopolysaccharidosis type IVA) NM_000512.3 (GALNS), mRNA. Homo sapiens UDP-N-acetyl-alpha-D- galactosamine:polypeptide N- GALNT1 NM_020474.2 acetylgalactosaminyltransferase 1 (GalNAc-T1) (GALNT1), mRNA. Homo sapiens growth differentiation factor 15 (GDF15), GDF15 NM_004864.1 mRNA. Homo sapiens Gen homolog 1, endonuclease (Drosophila) GEN1 NM_182625.2 (GEN1), mRNA. Homo sapiens geranylgeranyl diphosphate synthase 1 NM_00103727 GGPS1 (GGPS1), transcript variant 2, mRNA. 7.1 Homo sapiens gap junction protein, alpha 3, 46kDa (GJA3), GJA3 NM_021954.3 mRNA. Homo sapiens guanine nucleotide binding protein (G GNA13 NM_006572.3 protein), alpha 13 (GNA13), mRNA. Homo sapiens golgi phosphoprotein 3-like (GOLPH3L), GOLPH3L NM_018178.3 mRNA. Homo sapiens G protein-coupled estrogen receptor 1 NM_00103996 GPER (GPER), transcript variant 3, mRNA. 6.1 Homo sapiens general transcription factor IIE, polypeptide 1 GTF2E1 NM_005513.1 (alpha subunit, 56kD) (GTF2E1), mRNA. Homo sapiens general transcription factor IIH, polypeptide GTF2H1 NM_005316.2 1, 62kDa (GTF2H1), mRNA. Homo sapiens histone acetyltransferase 1 (HAT1), transcript HAT1 NM_003642.2 variant 1, mRNA. Homo sapiens
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