Effects of Acid Mine Drainage on Leaf Consumption and Fine
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EFFECTS OF ACID MINE DRAINAGE ON LEAF CONSUMPTION AND FINE PARTICULATE ORGANIC MATTER PRODUCTION BY THE CRAYFISH, ORCONECTES SANBORNII A thesis presented to the faculty of the College of Arts and Sciences of Ohio University In partial fulfillment of the requirements for the degree Master of Science Daniel A. Brown August 2007 This thesis titled EFFECTS OF ACID MINE DRAINAGE ON LEAF CONSUMPTION AND FINE PARTICULATE ORGANIC MATTER PRODUCTION BY THE CRAYFISH, ORCONECTES SANBORNII by DANIEL A. BROWN has been approved for the Department of Biological Sciences and the College of Arts and Sciences by Kelly S. Johnson Associate Professor of Biological Sciences Benjamin M. Ogles Dean, College of Arts and Sciences Abstract BROWN, DANIEL A., M.S., August 2007, Biological Sciences EFFECTS OF ACID MINE DRAINAGE ON LEAF CONSUMPTION AND FINE PARTICULATE ORGANIC MATTER PRODUCTION BY THE CRAYFISH, ORCONECTES SANBORNII (68 pp.) Director of Thesis: Kelly S. Johnson Crayfish feed on a wide range of plant and animal materials and are one of the dominant macroinvertebrates found in streams of the eastern United States. By consuming large amounts of leaf litter from riparian vegetation, crayfish are an important component of stream ecosystem dynamics. During the feeding process, leaves are shredded into coarse (CPOM) and fine particulate organic matter (FPOM) and become a basal food resource for many other stream-dwelling organisms. Acid mine drainage (AMD) is a significant ecological issue in southeastern Ohio and other areas where extensive mining for coal has occurred. The focus of this research was to determine the effects AMD has on the feeding rates of the crayfish, Orconectes sanbornii. This research consisted of two main components, a laboratory experiment and a field experiment. The laboratory experiment investigated the effects of changes in pH on crayfish leaf processing rates and FPOM production and compared the quality of leaves conditioned in AMD-impacted streams versus control streams (no AMD impact), through two laboratory feeding trials. Detrital processing by crayfish in AMD-polluted and control streams was quantified in situ using field enclosures. Macroinvertebrate colonization of the field enclosures was also recorded and compared between field sites. The results indicate that while crayfish are tolerant to acidic conditions, crayfish in low pH (4.0) consumed less leaf area and produced less FPOM than those in higher pH conditions (5.0-7.0). When subjected to pH 4.0, crayfish produced approximately 25% the amount of FPOM as those in pH 7.0. Leaf consumption varied in the two trials. Crayfish consumed more of the leaves conditioned in the control stream during the initial trial, but more of the leaves conditioned in an AMD-impacted stream in the second trial. This reflects the complex nature of the leaf conditioning process in streams, which can be influenced by many environmental factors. This research may prove to be beneficial as a reference for future research and reclamation projects. Approved: Kelly S. Johnson Associate Professor of Biological Sciences Acknowledgments I would like to give special thanks to my advisor, Kelly Johnson for her encouragement and steadfast support throughout my research. I would also like to extend thanks and much appreciation to my committee members, Donald Miles and Matthew White, for their valuable input and support. The recirculating tanks, without which the laboratory experiments would not have been possible, were provided by Morgan Vis. Thanks also to Willem Roosenburg for allowing me the use of his muffle furnace and to Warren Currie for the use of his pH meter. Roger Thoma provided verification of my crayfish identifications as well as valuable knowledge pertaining to crayfish. Special thanks go to Dave Elliot for his help with transportation to the field sites and his undeniable skill at making repairs to necessary equipment. I would also like to give thanks to Ashley Smith, Chad Kinney, Tracy Morman, Levi Rose, Breanna Harris, and Kelli Johnson for their help with various aspects along the way. Last but certainly not least, thanks to my family for their tremendous support. Thanks to my parents, David E. and Mary E. Brown, and my brother David J. Brown for his help with polishing this manuscript. 6 Table of Contents Page Abstract............................................................................................................................... 3 Acknowledgments............................................................................................................... 5 List of Tables ...................................................................................................................... 8 List of Figures..................................................................................................................... 9 CHAPTER ONE: LABORATORY EXPERIMENT ...................................................... 10 Introduction................................................................................................................... 10 Research Objectives...................................................................................................... 13 Methods......................................................................................................................... 15 Leaf Conditioning ..................................................................................................... 15 Acid Treatments........................................................................................................ 16 Capture and Use of Crayfish in the Laboratory Experiment .................................... 16 Quantifying Leaf Consumption ................................................................................ 18 Fine Particulate Organic Matter (FPOM) ................................................................. 19 Statistical Analyses of Leaf Consumption and FPOM Production........................... 20 Leaf Carbon and Nitrogen Content........................................................................... 21 Results........................................................................................................................... 22 Water Temperature Data........................................................................................... 22 Crayfish Body Size and Leaf Area Consumed ......................................................... 24 Cumulative Leaf Consumption................................................................................. 26 Leaf Consumption During Intervals ......................................................................... 33 FPOM Production..................................................................................................... 38 Holding Period Analysis - Laboratory Trial #2 ........................................................ 41 Notes Concerning pH and Leaf Conditioning Treatments ....................................... 41 Leaf Analyses............................................................................................................ 42 Discussion..................................................................................................................... 42 pH Effects on Leaf Consumption ............................................................................. 42 Leaf Conditioning Effects on Leaf Consumption..................................................... 43 CHAPTER TWO: FIELD EXPERIMENT ..................................................................... 46 Introduction................................................................................................................... 46 Research Objectives...................................................................................................... 46 Methods......................................................................................................................... 47 Calculations and Statistical Analysis........................................................................ 50 Results........................................................................................................................... 52 Jordan Run (control) ................................................................................................. 53 Truetown (control).................................................................................................... 53 Carbon Hill (control)................................................................................................. 54 Rock Run (AMD-impacted) ..................................................................................... 54 Job’s Hollow (AMD-impacted) ................................................................................ 55 Railroad 13 (AMD-impacted)................................................................................... 55 Comparison of Enclosures With and Without Crayfish ........................................... 56 Comparison Between Field Sites.............................................................................. 56 7 Discussion..................................................................................................................... 60 Leaf Pack Colonization by Macroinvertebrates........................................................ 60 The Role of Crayfish in Stream Ecosystems ............................................................ 60 Future AMD Management.......................................................................................