Bioinformatics Analysis of the Molecular Mechanisms Underlying Traumatic Spinal Cord Injury
8484 MOLECULAR MEDICINE REPORTS 17: 8484-8492, 2018 Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury SHU-JIE ZHAO, WEI ZHOU, JIAN CHEN, YONG-JUN LUO and GUO-YONG YIN Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China Received October 17, 2017; Accepted February 7, 2018 DOI: 10.3892/mmr.2018.8918 Abstract. Spinal cord injury (SCI) is a cause of disability. The which is primarily caused by traffic accidents, occurs when an present study aimed to investigate the molecular mechanisms external impact acutely damages the spinal cord, and may be involved in traumatic SCI. Transcriptome data under acces- temporally divided into four stages: i) Acute at <48 h following sion no. GSE5296, including 96 chips, were downloaded from injury; ii) subacute at 48 h-14 days following injury; iii) inter- the Gene Expression Omnibus database. The raw data were mediate at 14 days-6 months following injury; and iv) chronic normalized and differentially expressed genes (DEGs) were at >6 months following injury (1). Although numerous studies identified. Furthermore, Kyoto Encyclopedia of Genes and have focused on neuroprotective and neuroregenerative Genomes pathway and Gene Ontology enrichment analysis of therapies, no major breakthroughs have been achieved (3-5). up- and downregulated DEGs was performed. Additionally, Therefore, it is necessary to elucidate the underlying mecha- a protein-protein interaction network was constructed and nism of SCI. the expression patterns of different genes were determined. Pathophysiologically, the initial mechanical injury damages Compared with sham samples, there were 374, 707, 1,322, neurons and initiates a complex secondary injury cascade, 1,475, 1,724 and 1,342 DEGs identified at 0.5, 4, 24 and 72 h, leading to progressive cell death, ischemia and inflamma- and 7 and 28 days post-injury, respectively.
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