Nucleotide Sequence of the Ribosomal Protein Gene Cluster Adjacent to the Gene for RNA Polymerasesubunit
Proc. Natl. Acad. Sci. USA Vol. 76, No. 4, pp. 1697-1701, April 1979 Biochemistry Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit : in Escherichia coli (protein sequence/transcription initiation/transcription termination/codon usage/isoaccepting tRNAs) LEONARD E. POST*, GENEVA D. STRYCHARZ*, MASAYASU NOMURA*t, H. LEWISf, AND PATRICK P. DENNISf *Institute for Enzyfme Research and Departments of Genetics and Biochemistry, University of Wisconsin, Madison, Wisconsin 53706; and tDepartment of Biochemistry, Faculty of Medicine, University of British Columbia, 2075 Wesbrook Place, Vancouver, British Columbia V6T IW5, Canada Contributed by Masayasu Nomura, January 29,1979 ABSTRACT The 3072-nucleotide-long sequence of a seg- the EcoRI/HindII fragment to the right of the EcoRI ment from the 88-min region of the Escherichia coli chromo- 18.6%/4.4%-L site (unpublished work; cf. Fig. 1). The DNA some has been determined. The sequence covers the genes for and Gilbert ribosomal proteins LIi (rpK), LI (rpMA), Li (rplJ, and L7/L12 was sequenced by the method of Maxam (16). Dyad (rpIL), and the 5' end of the gene for the /3 subunit of RNA symmetry and codon frequency were analyzed using the polymerase (rpoB), along with the presumed regulatory regions computer program of Korn et al. (17), as modified by R. Lit- for these genes. The probable locations of the promoter for the tlewood (Biophysics Laboratory, University of Wisconsin, first two genes (the LII operon) and the promoter for the latter Madison) for the use on a Univac 1110 computer. three genes (the proximal part of the /3 operon) have been identified.
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