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Common Features of All Cells Bacterial
www.denniskunkel.com Tour of the Cell part 1 Today’s Topics • Finish Nucleic Acids Cells • Properties of all cells – Prokaryotes and Eukaryotes • Functions of Major Cellular Organelles – Information – Synthesis&Transport – Energy Conversion – Recycling – Structure and Movement Bacterial cell Animal Cell 9/12/12 (Prokaryote) (Eukaryote) 2 www.denniskunkel.com Common features of all cells • Plasma Membrane – defines inside from outside • Cytosol – Semifluid “inside” of the cell • DNA “chromosomes” - Genetic material – hereditary instructions • Ribosomes – “factories” to synthesize proteins 4 Plasma membrane Bacterial (Prokaryotic) Cell Ribosomes! Plasma membrane! Bacterial Cell wall! chromosome ! Phospholipid bilayer Proteins 0.5 !m! Flagella! No internal membranes 5 6 1 Figure 6.2b 1 cm Eukaryotic Cell Frog egg 1 mm Human egg 100 µm Most plant and animal cells 10 m µ Nucleus Most bacteria Light microscopy Mitochondrion 1 µm Super- 100 nm Smallest bacteria Viruses resolution microscopy Ribosomes 10 nm Electron microscopy Proteins Lipids 1 nm Small molecules Contains internal organelles 7 0.1 nm Atoms endoplasmicENDOPLASMIC RETICULUM reticulum (ER) ENDOPLASMIC RETICULUM (ER) NUCLEUS NUCLEUS Rough ER Smooth ER nucleus Rough ER Smooth ER Nucleus Plasma membrane Plasma membrane Centrosome Centrosome cytoskeletonCYTOSKELETON CYTOSKELETON Microfilaments You should Microfilaments Intermediate filaments know everything Intermediate filaments Microtubules in Fig 6.9 ribosomesRibosomes Microtubules Ribosomes cytosol GolgiGolgi apparatus apparatus Golgi apparatus Peroxisome Peroxisome In animal cells but not plant cells: In animal cells but not plant cells: Lysosome Lysosomes Lysosome Lysosomes Figure 6.9 Centrioles Figure 6.9 Centrioles Mitochondrion lysosome Flagella (in some plant 9sperm) Mitochondrion Flagella (in some plant10 sperm) mitochondrion Nuclear envelope Nucleus Nucleus 1 !m Nucleolus Chromatin Nuclear envelope: Inner membrane Outer membrane Pores Pore complex Rough ER Surface of nuclear envelope. -
Phosphatases and Differentiation of the Golgi Apparatus
J. Cell Sci. 4, 455-497 (1969) 455 Printed in Great Britain PHOSPHATASES AND DIFFERENTIATION OF THE GOLGI APPARATUS MARIANNE DAUWALDER, W. G. WHALEY AND JOYCE E. KEPHART The Cell Research Institute, Tlie University of Texas at Austin, Texas, U.S.A. SUMMARY Cytochemical techniques for the electron microscopic localization of inosine diphosphatase, thiamine pyrophosphatase, and acid phosphatase have been applied to the developing root tip of Zea mays. Following formaldehyde fixation the Golgi apparatus of most of the cells showed reaction specificity for IDPase and TPPase. Following glutaraldehyde fixation marked localiza- tion of IDPase reactivity in the Golgi apparatus was limited to the root cap, the epidermis, and the phloem. A parallelism was apparent between the sequential morphological development of the apparatus for the secretion of a polysaccharide product, the fairly direct incorporation of tritiated glucose into the apparatus to become a component of this product and the develop- ment of the enzyme reactivity. Acid phosphatase, generally accepted as a lysosomal marker, was found in association with the Golgi apparatus in only a few cell types near the apex of the root. The localization was usually in a single cisterna at the face of the apparatus toward which the production of secretory vesicles builds up and associated regions of what may be smooth endoplasmic reticulum. Since the cell types involved were limited regions of the cap and epidermis and some initial cells, no functional correlates of the reactivity were apparent. Despite the presence of this lysosomal marker, no structures clearly identifiable as ' lysosomes' were found and the lack of reaction specificity in the vacuoles did not allow them to be so defined. -
Questions in Cell Biology
Name: Questions in Cell Biology Directions: The following questions are taken from previous IB Final Papers on the subject of cell biology. Answer all questions. This will serve as a study guide for the next quiz on Monday 11/21. 1. Outline the process of endocytosis. (Total 5 marks) 2. Draw a labelled diagram of the fluid mosaic model of the plasma membrane. (Total 5 marks) 3. The drawing below shows the structure of a virus. II I 10 nm (a) Identify structures labelled I and II. I: ...................................................................................................................................... II: ...................................................................................................................................... (2) (b) Use the scale bar to calculate the maximum diameter of the virus. Show your working. Answer: ..................................................... (2) (c) Explain briefly why antibiotics are effective against bacteria but not viruses. ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... .............................................................................................................................................. -
Centrioles and the Formation of Rudimentary Cilia by Fibroblasts and Smooth Muscle Cells
CENTRIOLES AND THE FORMATION OF RUDIMENTARY CILIA BY FIBROBLASTS AND SMOOTH MUSCLE CELLS SERGEI SOROKIN, M.D. From the Department of Anatomy, Harvard Medical School, Boston, Massachusetts ABSTRACT Cells from a variety of sources, principally differentiating fibroblasts and smooth muscle cells from neonatal chicken and mammalian tissues and from organ cultures of chicken duodenum, were used as materials for an electron microscopic study on the formation of rudimentary cilia. Among the differentiating tissues many cells possessed a short, solitary cilium, which projected from one of the cell's pair of centrioles. Many stages evidently intermediate in the fashioning of cilium from centriole were encountered and furnished the evidence from which a reconstruction of ciliogenesis was attempted. The whole process may be divided into three phases. At first a solitary vesicle appears at one end of a centriole. The ciliary bud grows out from the same end of the centriole and invaginates the sac, which then becomes the temporary ciliary sheath. During the second phase the bud lengthens into a shaft, while the sheath enlarges to contain it. Enlargement of the sheath is effected by the repeated appearance of secondary vesicles nearby and their fusion with the sheath. Shaft and sheath reach the surface of the cell, where the sheath fuses with the plasma membrane during the third phase. Up to this point, formation of cilia follows the classical descriptions in outline. Subsequently, internal development of the shaft makes the rudi- mentary cilia of the investigated material more like certain non-motile centriolar derivatives than motile cilia. The pertinent literature is examined, and the cilia are tentatively assigned a non-motile status and a sensory function. -
Centrosome Positioning in Vertebrate Development
Commentary 4951 Centrosome positioning in vertebrate development Nan Tang1,2,*,` and Wallace F. Marshall2,` 1Department of Anatomy, Cardiovascular Research Institute, The University of California, San Francisco, USA 2Department Biochemistry and Biophysics, The University of California, San Francisco, USA *Present address: National Institute of Biological Science, Beijing, China `Authors for correspondence ([email protected]; [email protected]) Journal of Cell Science 125, 4951–4961 ß 2012. Published by The Company of Biologists Ltd doi: 10.1242/jcs.038083 Summary The centrosome, a major organizer of microtubules, has important functions in regulating cell shape, polarity, cilia formation and intracellular transport as well as the position of cellular structures, including the mitotic spindle. By means of these activities, centrosomes have important roles during animal development by regulating polarized cell behaviors, such as cell migration or neurite outgrowth, as well as mitotic spindle orientation. In recent years, the pace of discovery regarding the structure and composition of centrosomes has continuously accelerated. At the same time, functional studies have revealed the importance of centrosomes in controlling both morphogenesis and cell fate decision during tissue and organ development. Here, we review examples of centrosome and centriole positioning with a particular emphasis on vertebrate developmental systems, and discuss the roles of centrosome positioning, the cues that determine positioning and the mechanisms by which centrosomes respond to these cues. The studies reviewed here suggest that centrosome functions extend to the development of tissues and organs in vertebrates. Key words: Centrosome, Development, Mitotic spindle orientation Introduction radiating out to the cell cortex (Fig. 2A). In some cases, the The centrosome of animal cells (Fig. -
Studies on the Mechanisms of Autophagy: Formation of the Autophagic Vacuole W
Studies on the Mechanisms of Autophagy: Formation of the Autophagic Vacuole W. A. Dunn, Jr. Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, Florida 32610 Abstract. Autophagic vacuoles form within 15 min of tophagic vacuoles. All these results suggested that au- perfusing a liver with amino acid-depleted medium. tophagic vacuoles were not formed from plasma mem- These vacuoles are bound by a "smooth" double mem- brane, Golgi apparatus, or endosome constituents. An- brane and do not contain acid phosphatase activity. In tisera prepared against integral membrane proteins (14, Downloaded from http://rupress.org/jcb/article-pdf/110/6/1923/1059547/1923.pdf by guest on 26 September 2021 an attempt to identify the membrane source of these 25, and 40 kD) of the RER was found to label the in- vacuoles, I have used morphological techniques com- ner and outer limiting membranes of almost all na- bined with immunological probes to localize specific scent autophagic vacuoles. In addition, ribophorin II membrane antigens to the limiting membranes of was identified at the limiting membranes of many na- newly formed or nascent autophagic vacuoles. Anti- scent autophagic vacuoles. Finally, secretory proteins, bodies to three integral membrane proteins of the rat serum albumin and alpha2o-globulin, were localized plasma membrane (CE9, HA4, and epidermal growth to the lumen of the RER and to the intramembrane factor receptor) and one of the Golgi apparatus space between the inner and outer membranes of some (sialyltransferase) did not label these vacuoles. Inter- of these vacuoles. The results were consistent with the nalized epidermal growth factor and its membrane formation of autophagic vacuoles from ribosome-free receptor were not found in nascent autophagic vacu- regions of the RER. -
Golgi-Apparatus.Pdf
GOLGI APPARATUS Cell Biology/B. Sc. Part III By: Dr. Bibha Kumari Dept. of Zoology Magadh Mahila College, Patna Email: [email protected] Golgi Apparatus • The Golgi apparatus (GA), also called Golgi body or Golgi complex and • found universally in both plant and animal cells, • is typically comprised of a series of five to eight cup- shaped, membrane-covered sacs called cisternae that look something like a stack of deflated balloons. • It is another packaging organelle like the endoplasmic reticulum (ER). It was named after Camillo Golgi (1897), an Italian biologist. • While layers of membranes may look like the rough ER, they have a very different function. Golgi….. • In some unicellular flagellates, however, as many as 60 cisternae may combine to make up the Golgi apparatus. • Similarly, the number of Golgi bodies in a cell varies according to its function. • Animal cells generally contain between ten and twenty Golgi stacks per cell, which are linked into a single complex by tubular connections between cisternae. • This complex is usually located close to the cell nucleus. Position in the cell Structure of Golgi Apparatus • A Golgi apparatus is composed of flat sacs known as cisternae. • The sacs are stacked in a bent, semicircular shape. • Each stacked grouping has a membrane that separates its insides from the cell's cytoplasm. • Golgi membrane protein interactions are responsible for their unique shape. • These interactions generate the force that shapes this organelle. Structure…. Structure…. Structure…… • The Golgi apparatus is very polar. • Membranes at one end of the stack differ in both composition and in thickness from those at the other end. -
Dynamics of Ribosome Association with the Endoplasmic Reticulum Membrane
Dynamics of Ribosome Association with the Endoplasmic Reticulum Membrane Dissertation zur Erlangung des Doktorgrades der Fakultaet fuer Biologie der Ludwig-Maximilians-Universitaet Muenchen vorgelegt von Dipl.-Biochem. Julia Schaletzky Mai 2006 Die vorliegende Arbeit wurde an der Harvard Medical School in Boston, MA (USA) unter der Anleitung von Prof. Tom Rapoport durchgefuehrt. Die dreidimensionale Struktur der von mir isolierten Komplexe wurde in Kollaboration mit Prof. Chris Akey (Boston University, USA) unter Anleitung von Dr. Jean-François Ménétret durch Cryo-Elektronenmikroskopie ermittelt. Die vorliegende Arbeit wurde zur Beurteilung eingereicht am 01.06.2006. Gutachter: Prof. Dr. J. Soll PD Dr. E. Schleiff Prof. Dr. M. Hayashi Prof. Dr. K. Jung Rigorosum: 20.09.2006 Ehrenwoertliche Versicherung Hiermit versichere ich, dass ich die vorliegende Arbeit selbstaendig verfasst und keine anderen als die von mir angegebenen Quellen und Hilfsmittel verwendet habe. Ferner erklaere ich, dass ich anderweitig nicht versucht habe, eine Dissertation einzureichen oder mich einer Doktorpruefung zu unterziehen. Die vorliegende Arbeit ist nicht als Ganzes oder in Teilen einer weiteren Pruefungskommission vorgelegt worden. Boston, 12.05.06 ........................................ (Julia Schaletzky) ACKNOWLEDGEMENTS I would like to thank Prof. Tom Rapoport for his continuing support and advice during the supervision of my project, and for being a great teacher and mentor. I would like to express my gratitude to Prof. Juergen Soll for generously agreeing to be my advisor and to lead my PhD committee. Prof. Stefan Jentsch deserves thanks for agreeing to examine my PhD thesis and for his constant support and help during the pursuit of my PhD. I am also grateful to Prof. -
Membrane Structure and Function
Chapter 7 Membrane Structure and Function Lecture Outline Overview: Life at the Edge • The plasma membrane separates the living cell from its surroundings. • This thin barrier, 8 nm thick, controls traffic into and out of the cell. • Like all biological membranes, the plasma membrane is selectively permeable, allowing some substances to cross more easily than others. • The formation of a membrane that encloses a solution different from the surrounding solution while still permitting the uptake of nutrients and the elimination of waste products was a key event in the evolution of life. • The ability of the cell to discriminate in its chemical exchanges with its environment is fundamental to life. • It is the plasma membrane and its component molecules that make this selectivity possible. Concept 7.1 Cellular membranes are fluid mosaics of lipids and proteins. • The main macromolecules in membranes are lipids and proteins, but carbohydrates are also important. • The most abundant lipids are phospholipids. • Phospholipids and most other membrane constituents are amphipathic molecules, which have both hydrophobic and hydrophilic regions. Membrane models have evolved to fit new data. • The arrangement of phospholipids and proteins in biological membranes is described by the fluid mosaic model. • In this model, the membrane is a fluid structure with a “mosaic” of various proteins embedded in or attached to a double layer (bilayer) of phospholipids. • Models of membranes were developed long before membranes were first seen with electron microscopes in the 1950s. • In 1915, membranes isolated from red blood cells were chemically analyzed and found to be composed of lipids and proteins. • In 1925, E. -
The Role of the Cytoskeleton in the Structure and Function of the Golgi Apparatus Gustavo Egea and Rosa M
The role of the cytoskeleton in the structure and function of GA * 1 The role of the cytoskeleton in the structure and function of the Golgi apparatus Gustavo Egea and Rosa M. Ríos Introduction Cellular organelles in mammalian cells are individualized membrane entities that often become spherical. The endoplasmic reticulum (ER) and the Golgi apparatus (GA) are exceptions to this rule, as they are respectively made up of a continuous tubular network and a pile of flat disks. Their unique shapes are regulated by molecular elements (Kepes et al. 2005; Levine and Rabouille 2005), including the cytoskeleton. All cytoskeletal elements, together with cytoskeleton-associated motors and non-motor proteins, have a role in the subcellular positioning, biogenesis and function of most organelles, being particularly relevant in the GA. The GA is the central organelle of the eukaryotic secretory pathway. While its basic function is highly conserved, the GA varies greatly in shape and number from one organism to another. In the simplest organisms like budding yeast Saccharomyces cerevisiae, the organelle takes the form of dispersed cisternae or isolated tubular networks (Preuss et al. 1992; Ram- bourg et al. 2001). Unicellular green alga (Henderson et al. 2007) and many protozoa like Toxoplasma gondii (Pelletier et al. 2002) and Trypanosoma brucei (He 2007; He et al. 2004) contain a single pile of flattened cisternae aligned in parallel. The organization of the GA in this manner is referred to as a Golgi stack, which usually contains two regions: one central and poorly fenestrated (compact) and other lateral and highly fenestrated (non-com- pact) (Kepes et al. -
Role of Microtubules in the Distribution of the Golgi Apparatus
Proc. Nati Acad. Sci. USA Vol. 80, pp. 4286-4290, July 1983 Biochemistry Role of microtubules in the distribution of the Golgi apparatus: Effect of taxol and microinjected anti-a-tubulin antibodies (organelle organization/cytoskeleton) JURGEN WEHLAND*, MARYANNA HENKARTt, RICHARD KLAUSNERt, AND IGNACIO V. SANDOVALt§ *Laboratory of Molecular Biology and tImmunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20205; and *Laboratory of Biochemistry and Metabolism, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20205 Communicated by Gilbert Ashwell, April 21, 1983 ABSTRACT Immunofluorescence microscopy reveals that both organization and distribution of both microtubules and the Gol- microtubule organizing center (MTOC) and Golgi apparatus are gi apparatus were studied by dual indirect immunofluores- contained in the same perinuclear area of A549 cells in inter- cence microscopy as described (6). Cells were permeabilized by phase. The cells display long microtubules stretching radially from immersion in cold methanol (-20°C) for 2 min. The Golgi ap- the MTOC to the plasma membrane. Treatment of cells with taxol paratus was studied by using a rabbit monospecific antibody results in polymerization of microtubules without relation to the was MTOC and formation of microtubule bundles predominantly lo- raised against the Golgi enzyme ,B-galactosyltransferase and calized in the cell periphery. After incubation with taxol, the -
Lysosomal Biology and Function: Modern View of Cellular Debris Bin
cells Review Lysosomal Biology and Function: Modern View of Cellular Debris Bin Purvi C. Trivedi 1,2, Jordan J. Bartlett 1,2 and Thomas Pulinilkunnil 1,2,* 1 Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4H7, Canada; [email protected] (P.C.T.); jjeff[email protected] (J.J.B.) 2 Dalhousie Medicine New Brunswick, Saint John, NB E2L 4L5, Canada * Correspondence: [email protected]; Tel.: +1-(506)-636-6973 Received: 21 January 2020; Accepted: 29 April 2020; Published: 4 May 2020 Abstract: Lysosomes are the main proteolytic compartments of mammalian cells comprising of a battery of hydrolases. Lysosomes dispose and recycle extracellular or intracellular macromolecules by fusing with endosomes or autophagosomes through specific waste clearance processes such as chaperone-mediated autophagy or microautophagy. The proteolytic end product is transported out of lysosomes via transporters or vesicular membrane trafficking. Recent studies have demonstrated lysosomes as a signaling node which sense, adapt and respond to changes in substrate metabolism to maintain cellular function. Lysosomal dysfunction not only influence pathways mediating membrane trafficking that culminate in the lysosome but also govern metabolic and signaling processes regulating protein sorting and targeting. In this review, we describe the current knowledge of lysosome in influencing sorting and nutrient signaling. We further present a mechanistic overview of intra-lysosomal processes, along with extra-lysosomal processes, governing lysosomal fusion and fission, exocytosis, positioning and membrane contact site formation. This review compiles existing knowledge in the field of lysosomal biology by describing various lysosomal events necessary to maintain cellular homeostasis facilitating development of therapies maintaining lysosomal function.