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Icvg 2009 Part I Pp 1-131.Pdf 16th Meeting of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG XVI) 31 August - 4 September 2009 Dijon, France Extended Abstracts Le Progrès Agricole et Viticole - ISSN 0369-8173 Modifications in the layout of abstracts received from authors have been made to fit with the publication format of Le Progrès Agricole et Viticole. We apologize for errors that could have arisen during the editing process despite our careful vigilance. Acknowledgements Cover page : Olivier Jacquet Photos : Gérard Simonin Jean Le Maguet ICVG Steering Committee ICVG XVI Organising committee Giovanni, P. MARTELLI, chairman (I) Elisabeth BOUDON-PADIEU (INRA) Paul GUGERLI, secretary (CH) Silvio GIANINAZZI (INRA - CNRS) Giuseppe BELLI (I) Jocelyne PÉRARD (Chaire UNESCO Culture et Johan T. BURGER (RSA) Traditions du Vin, Univ Bourgogne) Marc FUCHS (F – USA) Olivier JACQUET (Chaire UNESCO Culture et Deborah A. GOLINO (USA) Traditions du Vin, Univ Bourgogne) Raymond JOHNSON (CA) Pascale SEDDAS (INRA) Michael MAIXNER (D) Sandrine ROUSSEAUX (Institut Jules Guyot, Univ Gustavo NOLASCO (P) Bourgogne) Denis CLAIR (INRA) Ali REZAIAN (USA) Dominique MILLOT (INRA) Iannis C. RUMBOS (G) Xavier DAIRE (INRA – CRECEP) Oscar A. De SEQUEIRA (P) Mary Jo FARMER (INRA) Edna TANNE (IL) Caroline CHATILLON (SEDIAG) Etienne HERRBACH (INRA Colmar) René BOVEY, Honorary secretary Jean Le MAGUET (INRA Colmar) Honorary committee members Session convenors A. CAUDWELL (F) D. GONSALVES (USA) Michael MAIXNER H.-H. KASSEMEYER (D) Olivier LEMAIRE G. KRIEL (RSA) Etienne HERRBACH D. STELLMACH, (D) Élisabeth BOUDON-PADIEU A. TELIZ, (Mex) Sandrine ROUSSEAUX A. VUITTENEZ (F) Pascale SEDDAS B. WALTER (F). Invited speakers to ICVG XVI Giovanni P. MARTELLI Paul GUGERLI Valerian V. DOLJA Sead SABANADZOVIC Gérard DEMANGEAT Xavier FOISSAC Ilan SELA Cristina CABALEIRO Pasquale SALDARELLI Conference secretariat Sophie NOLLOT - Sandrine GIORDANA Alice MORISOT - Isabelle CARRÉ INRA – UMR PME - BP 86510 – 21650 DIJON Cedex Phone : 33 (0)380 69 34 40 / 33 (0)380 69 30 00 Fax: 33-(0)380 69 37 53 E-mail : [email protected] https://colloque2.inra.fr/icvg16 ICVG website : http://www.icvg.ch AVANT-PROPOS Nous sommes très heureux que le choix du Comité de direction de l'ICVG se soit porté sur Dijon pour la tenue de sa 16ème conférence internationale. C'est la deuxième fois seulement depuis sa création que l'ICVG se réunit en France et la première fois en Bourgogne, terre de vigne et de vins. Le Comité de direction de l'ICVG œuvre depuis sa création pour promouvoir les recherches sur ces maladies de dépérissement de la vigne qui déprécient insidieusement les productions des meilleurs cépages dans leur qualité et leur rendement. Que le nombre de participants à ces conférences ne cesse de croître est un gage du succès de son entreprise. Vous découvrirez ici le nombre et la qualité des présentations scientifiques du cru 2009 de l'ICVG. Merci à tous ceux qui y ont contribué et aux partenaires et sponsors qui l'ont grandement aidé. Bienvenue en Bourgogne et Bienvenue dans ces pages! FOREWORD We are very happy that the choice of the Steering Committee of ICVG was turned towards Dijon for the venue of its 16th international conference. This is only the second time since its foundation that ICVG meets in France, the first time in Burgundy, a land of vineyards and wines. From its very early days, the Steering committee of ICVG has dedicated its efforts to promote research on grapevine decline diseases that insidiously affect the production of best cultivars both in quality and yield. That the number of delegates to conferences steadily increases demonstrates the success of their enterprise. You will discover herein the number and quality of the scientific contributions of vintage 2009 of ICVG. Thanks to all who contributed and to partners and sponsors who greatly helped. Welcome in Burgundy and Welcome within these pages! Title : 16th Meeting of the International Council for the Study of Virus and virus-like diseases of the Grapevine – Extended abstracts Editor : Elisabeth Boudon-Padieu Publisher : Le Progrès Agricole et Viticole - ISSN 0369-8173 Correct citation Extended abstracts 16th Meeting of ICVG, Dijon, France, 31 Aug-4 Sept 2009, (pg) – (pg) REMERCIEMENTS Nous remercions le Conseil régional de Bourgogne et son président M. le Sénateur François Patriat, pour son soutien financier à l'organisation de la conférence et son hospitalité dans sa salle de conférence. Nous sommes très redevables à la Communauté de l'Agglomération Dijonnaise (Comadi) en la personne de son président, M. François Rebsamen, Sénateur Maire de Dijon, et de ses collaborateurs pour avoir accepté de mettre à notre disposition pendant trois jours les magnifiques installations du Grand Dijon et toutes les facilités d'accueil d'une assemblée aussi nombreuse. Nous voulons ici remercier Monsieur Olivier Le Gall, Chef du département Santé des plantes et Environnement de l'INRA, pour le soutien accordé par le département, et Monsieur Jacques Caneill, président du Centre INRA de Dijon ainsi que Madame Vivienne Gianninazzi-Pearson, Directrice de l'Unité de recherches Plante Microbe Environnement INRA – CNRS – Université de Bourgogne à Dijon, pour nous avoir accordé l'hospitalité de la logistique et du secrétariat indispensables à la préparation de cette conférence. La publication des Actes n'aurait pu se faire sans la confiance de la Chaire Unesco Culture et traditions du Vin de l'Université de Bourgogne, en la personne de Madame le Professeur Jocelyne Pérard. Au sein du Comité d'organisation chacun a joué une partie importante dans une atmosphère cordiale et enthousiaste. Enfin le soutien des organisations et entreprises viticoles de Bourgogne et de Franche-Comté : Bureau Interprofessionnel des Vins de Bourgogne, Chambre d'Agriculture de Saône et Loire, Société de Viticulture du Jura, Pépinières Guillaume, Domaine Latour, nous a été acquis sans réserve. Qu'elles soient toutes remerciées, en particulier pour leur accueil durant les visites techniques. Merci aux entreprises Bioreba, Sediag et Bayer Crop Protection qui ont manifesté leur intérêt pour la vaste audience internationale que constitue notre conférence, à la SNCF Gare de Dijon et à l'Office de tourisme de la ville de Dijon qui ont contribué à l'accueil de nos visiteurs proches et lointains. ACKNOWLEDGEMENTS We thank the Regional Council of Burgundy and its president, the Senator François Patriat, for their financial support to the organisation of the conference and their hospitality in the Conference Hall. We are most indebted to the Communauté de l'Agglomération Dijonnaise (Comadi), to its president, Senator and Mayor of Dijon François Rebsamen, and its collaborators for having let us the disposal during three days of the beautiful hall of the Grand Dijon and all facilities necessary to welcome such a large assembly. We want to thank here Dr. Olivier Le Gall, Head of the Department Plant health and Environment of INRA for the support given by the Department, Dr Jacques Caneill, president of the INRA Centre of Dijon, and Dr. Vivienne Gianninazzi-Pearson, Head of the Research Unit Plant Microbe Environment INRA – CNRS – Université de Bourgogne in Dijon, for hosting the logistics and secretary necessary for the organisation of the Conference Publication of the Extended Abstracts would not have been possible without the confidence of the Unesco Chair Culture and Traditions of Wine of University of Burgundy, in the person of its Head, Mrs the Professor Jocelyne Pérard. Every one in the Organisation committee has played its part with an enthusiastic and hearty complicity. Profession and industry of viticulture of Burgundy and Franche-Comté : Bureau Interprofessionnel des Vins de Bourgogne, Chambre d'Agriculture de Saône et Loire, Société de Viticulture du Jura, Pépinières Guillaume, Domaine Latour, have fully supported us. We are most thankful to them all, especially for their reception during technical visits. Bioreba, Sediag and Bayer Crop Protection have readily shown their interest towards the vast international audience of our conference. The SNCF Gare de Dijon and Tourism Office of Dijon city have contributed to the welcome of our visitors from near and remote countries. Content - Sommaire Page Partners and sponsors – Partenaires et parrainage 2 ICVG Steering Committee – ICVG XVI Organisation 4 Foreword – Avant propos 5 Acknowledgements - Remerciements 6 Content - Sommaire 7 Session I - Introductory keynotes Oral Martelli G. P. 15 Grapevine Virology Highlights 2006-09 Gugerli P. 24 25 years of serological identification of grapevine leafroll-associated viruses: antiserum and monoclonal antibodies to GLRaV-1 to GLRaV- 9 Dolja V. V. 29 New developments in understanding gene functions and evolution of the grapevine Closteroviruses Session II – Detection – Plant material and Virus source Oral Invited lecture 32 Sabanadzovic S. Viruses of native Vitis germplasm in the Southeastern united states Comparison of High Throughput Low Density Arrays, Rt-PCR and Real-Time Taqman® RT-PCR in the 36 detection of grapevine viruses Osman F., C. Leutenegger, D.A. Golino, A. Rowhani Grapevine viruses in Chile: Multi-parallel detection based on metagenomic strategies 38 Engel E. A., P. F. Escobar, P. Rivera, P. D.T. Valenzuela. Grapevine virus collection at Nyon: A contribution to a putative network of a worldwide grapevine virus 40 reference collection Gugerli P., J-J. Brugger, M-E. Ramel, S. Besse. Poster 01 Grapevine virus Q: The first plant virus with a permuted active site of RNA-dependent RNA polymerase 42 Sabanadzovic S., N. Abou Ghanem-Sabanadzovic, A. E. Gorbalenya Poster 02 Production of monoclonal antibodies to Grapevine leafroll- associated virus 9 (GLRaV-9) 44 Gugerli P., S. Rigotti, M-E. Ramel, N. Habili, A. Rowhani, W. Bitterlin, S. Besse. Poster 03 Detection of viruses in seeds of infected grapevines with an emphasis on Grapevine rupestris stem pitting- 46 associated virus Habili N., R. Davies, J. Randles. Poster 04 Real time PCR for sensitive reliable grapevine virus detection 48 Malan S., M-J.
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