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Study of the Role of the Histone Demethylase JMJD3 As a Transcriptional Regulator Raquel Fueyo Arévalo

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Study of the Role of the Histone Demethylase JMJD3 As a Transcriptional Regulator Raquel Fueyo Arévalo ADVERTIMENT. Lʼaccés als continguts dʼaquesta tesi queda condicionat a lʼacceptació de les condicions dʼús establertes per la següent llicència Creative Commons: http://cat.creativecommons.org/?page_id=184 ADVERTENCIA. El acceso a los contenidos de esta tesis queda condicionado a la aceptación de las condiciones de uso establecidas por la siguiente licencia Creative Commons: http://es.creativecommons.org/blog/licencias/ WARNING. The access to the contents of this doctoral thesis it is limited to the acceptance of the use conditions set by the following Creative Commons license: https://creativecommons.org/licenses/?lang=en Strangers on this road we are on, we are not two we are one. The Kinks, “Strangers” A mi compañero vital Alberto Gamazo, por hacerme más libre. Acknowledgements Acknowledgements “Though no man can draw a stroke between the confines of day and night, yet light and darkness are upon the whole tolerably distinguishable” (Edmund Burke). Esta pomposa frase resume lo que estos años de tesis doctoral me han aportado como investigadora: una inconmensurable resistencia al fracaso y toneladas de pragmatismo. Con esto quiero comenzar a dar las gracias a Marian Martínez-Balbás, por haberme formado en competencias científicas y en competencias transversales como la idiosincrasia de la ciencia y sus científicos. El hecho de que no me pueda imaginar dedicándome a otra cosa en el futuro te lo debo en gran medida a ti. Gracias por las discusiones regadas con café (quizás más café del que deberíamos) y por no estar siempre de acuerdo conmigo. Eres una de las personas que más ha marcado mi vida y si retrocediera en el tiempo, elegiría de nuevo tu laboratorio para hacer la tesis. Gracias por motivarme de manera extraordinaria, apoyarme y darme espacio para crear. Mis éxitos científicos siempre serán en gran medida tuyos. Continuaré dando las gracias a mi familia. Primeramente, a mi madre, que desde mi nacimiento sacrificó su bienestar por el mío y luchó para que las dos saliéramos ilesas en una carrera llena de obstáculos. Gracias por enseñarme a ser generosa y solidaria y por transmitirme tu buen humor. Lo único que no te agradezco es el gen de la impaciencia, no ha sido un buen complemento para esta tesis, ¿o sí?, jaja. Aunque nuestros mundos sean ahora tan diferentes, siempre serás un orgullo para mí, nunca lo olvides. Continúo dando las gracias a mis abuelas y a mi abuelo, que aunque lleven años muertos siguen presentes en mi mente como en mi infancia. Por un lado, gracias a mi abuela María “la Andaluza” por haberme querido sin medida y por haber participado activamente en mi educación. Solo lamento no haber podido enseñarte a leer y aunque no sirva de nada, de manera simbólica prometo que todo lo que la lectura me enseñe lo aplicaré con valor y estrategia como tú hiciste durante tu vida. Gracias también a mi abuelo Juan por confiar ciegamente en mí, ¡cuánto me avergonzaba que presumieras de nieta!... Gracias por hacerme sentir que te fortalecía hasta el último día de tu vida, te echo extraordinariamente de menos. Gracias a mi otra abuela María. Con los años me he dado cuenta de todos los valores feministas que me inculcabas y lamento infinitamente que los tuvieras que aprender de una manera atroz. Nunca se te agradecerá lo suficiente el esfuerzo que hiciste por todos los nietos y el amor que nos diste a todos y a cada uno, eras una persona admirable. Sigo con este emocionante ejercicio de memoria para dar las gracias a mi padre. Tantos años escuchando que somos tan parecidos me han hecho darme cuenta de lo difícil que es mantenerse cuerdo siendo idealista. Gracias por inculcarme valores éticos y este ansia por saber. Perdono tus fallos y utilizo tus fracasos como motivación en mi manera de vivir, ojalá pueda seguir compartiendo contigo mis vivencias muchos años. No podría terminar esta sección de agradecimientos sin dar las gracias a mi tío José Luis, que a pesar de ostentar el record mundial de ahijadas ha tenido tiempo y ganas de cuidar de mí. También gracias a mis tías Mari Carmen y Juana por ser mucho más que mis tías y celebrar mis triunfos como si fueran suyos. Gracias a José por tener ese corazón de oro, valoro mucho lo que has hecho por mí y sobre todo lo que haces por mi madre, te pido perdón por las veces que como adolescente me puse un poco rabuda jaja. Ahora que somos familia, tengo que añadir aquí los agradecimientos a mis suegros Conchi y Nacho. Gracias por acogerme sin peros y por contribuir con vuestra experiencia vital a mi vida. Acknowledgements Paso ahora a dar las gracias a mis compañeras del laboratorio de Marian. ¡Cuántas horas hemos compartido! Gracias a todas las que habéis pasado por ahí durante estos años, no hay ni una sola de vosotras que no me haya hecho aprender. Empiezo por estricto orden cronológico. Gracias a Conchi por ser mi primera mentora, el paso del tiempo me ha mostrado el estrés que supone estar en el último año de tesis y perdono que alguna vez fueras un poco impaciente conmigo. Gracias por enseñarme las técnicas de laboratorio y la responsabilidad que ser investigadora conlleva, te deseo lo mejor en el mundo científico, porque tu pasión lo merece. Continúo dando las gracias a Alejandra, la persona de la que más aprendí en el laboratorio. Gracias por no mirar el reloj a la hora de discutir sobre mi proyecto, por despertar en mí la curiosidad por la biología evolutiva y por reafirmarme en mi visión del mundo, espero que la vida te depare muchas alegrías. Gracias Mari por reírte de todas las bobadas que he dicho durante los años y por hacerme reír con tu amor por Jaume y por el actor, te deseo lo mejor en la vida y que sueñes todo lo que te propongas, como Alejandra y yo jaja...Gracias a Miriam Balada por hacerme crecer como mentora y hacerme sentir que podía transmitir mi motivación por la ciencia. Ευχαριώ a Stella, que a pesar de que siempre decimos mal su nombre nos quiere igual. Gracias por escucharme durante estos años y ayudarme con mi proyecto. Quiero decirte que envidio tu exactitud haciendo los experimentos... ¡ojalá algún día me salgan a mí así los controles!, me lo he pasado genial contigo este tiempo. Grazie mille a Simona por enseñarme que los postres pueden llevar pistacho... ¡es broma! Gracias por ser tan buena compañera y valorar mis consejos, ¡da gusto trabajar con gente como tú!, espero que no te hayan molestado mucho mis ganas constantes de hablar jaja. Gracias a Claudia por enseñarme tantos trucos técnicos y por enseñarme lo valioso que es tener las cosas claras, fue un placer trabajar contigo. Gracias a Marta por hacerme sentir tan orgullosa con sus logros durante el Máster y por estar tan loca como para querer trabajar con JMJD3 y los enhancers. Valoro muchísimo tu prudencia y madurez y te deseo lo mejor en esta aventura que comienzas. Por último, doy las gracias a Elia por ser tan risueña y alegrarse de mis triunfos a pesar de que nos conozcamos poco, seguro que tendrás un futuro brillante. No nos engañemos, en el IBMB todos nos debemos favores y eso es maravilloso. Agradezco aquí el buen ambiente que hay entre estudiantes y postdocs y ojalá que en mis futuros laboratorios sea la mitad de bueno. Empiezo dando las gracias a Sonia Najas, diosa de la corticogenesis. Gracias por valorarme tanto como científica y estar siempre ahí cuando te he necesitado. Espero que seas capaz de ver la gran investigadora que hay en ti y te confieso que cada vez que diseño un experimento intento ser paciente y reflexiva como tú. Gracias a Juan Arranz por ser tan buen compañero y por hacerme reír hasta el infinito, a Mariajo por ser tan pragmática, simpática y sobre todo, por odiar la homeopatía como yo jaja (aunque no está la cosa para reírse...). Gracias a Isa por quedarse siempre hasta tan tarde y no hacerme sentir una loca jaja, en serio, gracias por ser tan buena compañera. Muchas gracias a todos los del laboratorio de Elisa: Lucía, Elena, Murielle, José...y especialmente a Susana por ser la persona más amable que conozco y a Gwen por hacerme ser mejor científica y hacerme tener en cuenta la complejidad del sistema nervioso a la hora de interpretar mis resultados. Por supuesto gracias a Elisa que me ha hecho sentir muy apreciada en el laboratorio ¡nunca pierdas esa pasión que te caracteriza! Gracias al laboratorio de Albert Jordan por esos seminarios tan divertidos y especialmente gracias a Andrea por ser una tía de puta madre y a Carles por ser el adalid de la transmisión oral de cotilleos científicos de hoy, ayer y de siempre. Me encantan tus historias de como se hacía X técnica hace años o de cotilleos de los científicos, esos seres. Gracias a Ismael por ser Acknowledgements un derroche de bondad. Gracias a Carme Gallego por enseñarme a clonar, aunque quizás no te acuerdes. Gracias a Sara Gutiérrez, por enseñarme que hay muchas maneras de ser científica. Sobre todo, gracias a Raúl por todas las horas de conversación acerca de si los científicos estamos locos o no y por estar en el laboratorio días y horas intempestivas confirmándome que efectivamente, muy cuerdos no estamos. Ojalá nuestros caminos se vuelvan a cruzar, eres un gran amigo y compañero. Por último, quiero dar las gracias a los del laboratorio de Joaquím Roca, sois todos majísimos y siempre me habéis ayudado, sobre todo gracias a Toni que es una excelente persona y un gran conversador, ¡nos vemos en el Instituto de cromatina de Mallorca! También, aunque no haya participado directamente en mi tesis quiero dar las gracias a Leonor Calopa, la mujer que hace que el IBMB se mantenga en pie, gracias por toda la ayuda administrativa.
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