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Molecular-Weight Markers ・DNA Step Ladders ・OneSTEP Ladders ・Ladders for 10minute separation ・Wide Range Ladders ・Markers ＆ OneSTEP Markers ・RRNANA SSizei SStandardt d Markers ・DGGE Markers ・RNA Step Ladder ・PCR Marker Makers ・Loading Buffers Molecular-Weight Markers ・EasyEasy SeparatorSeparatorTM ・Negative Gel Stain MS Kit ・SuperSepTM Ace ・Prestain markers ・SuperSepTM DNA ・ Western marker ・ Biotinylated marker ・ Markers for CBB dyeing co.jp DNA Ladder DNA Ladder (1~6) Ready With dye and glycerol already DNA Step Ladder to added, it is ready to be applied to use for low-molecular-range analysis. the agarose gel. 10 bp DNA Step 25 bp DNA Step 25 bp DNA Step 50 bp DNA Step Ladder (10-100 bp) Ladder (25-300 bp) Ladder (25-500 bp) Ladder (50-800 bp) －20℃ （bp） －20℃ （bp） －20℃ （bp）（ng） －20℃ （bp） 500 60 100 300 800 400 60 90 275 700 300 60 600 6xBlue/ 80 6x 250 6xBlue/ Orange Loading 225 250 60 Orange 500 Loading 70 Dye Loading Dye 200 200 120 Dye 400 60 175 175 80 300 50 150 150 80 125 Loading: 40 Loading: Loading: 125 80 Loading: 200 30 100 100 120 1µL/lane 5µL/lane 5µL/lane 5µL/lane 150 20 75 75 80 10 50 50 80 100 25 25 80 50 1 2 3 4 3.5% Agarose 2% Agarose 2% Agarose 1% Agarose OneSTEP Ladder (7~12) for simultaneous measurement of DNA size and amount. DNA Step Ladder 100 bp DNA Step Ladder OneSTEP Ladder 50 OneSTEP Ladder 100 (10-300 bp) (0.1-1.5 kbp) (0.05-2 kbp) (0.1-2 kbp) （bp） （bp）（bp）（ng/5µL） （bp）（ng/5µL） Ready Ready Ready to 300 －20℃ 1,500 to 2,000 100 to 2,000 100 use use 1,500 75 use 1,800 90 200 1,000 1,000 50 900 1,600 80 －20℃ 900 45 1,400 70 150 6x 800 800 40 Loading 700 35 1,200 60 100 Dye 1,000 100 75 717 600 30 500 75 900 45 6x 50 600 Loading 400 22.5 800 40 Dye 35 500 450 20 700 35 350 17.5 600 30 25 400 300 15 20 250 37.5 500 50 15 286 200 10 400 20 10 150 7.5 300 15 200 100 10 200 10 100 10 100 50 100 2% Agarose 3% Agarose 21 3% Agarose 21 5% Agarose 21 Loading: Loading: Concentration: Concentration: 5 5-10µL/lane 6 5µL/lane 7 0.69µg/5µL 8 0.755µg/5µL OneSTEP Ladder 500 OneSTEP Ladder 1 kb OneSTEP Ladder Gene Ladder 100 (0.5-5 kbp) (1-10 kbp) Supercoiled Plasmid (0.1-2 kbp) Ready （bp） Ready （kbp） Ready （kbp） Ready （bp）（ng/5µL） to to to to use 5,000 use use use 4,500 10.0 2,000 100 4,000 9.0 10.0 8.0 －20℃ 1,500 75 3,500 9.0 3,000 7.0 8.0 1,000 50 2,500 6.0 7.0 900 45 2,000 5.0 6.0 4.0 800 40 5.0 700 35 1,500 3.0 4.0 600 30 2.0 500 75 1,000 3.0 400 40 300 30 200 20 500 1.0 100 100 1% Agarose S 2% Agarose S 1% Agarose S 1% Agarose S each fragment: each fragment: each fragment: Concentration: 9 50ng/5µL 10 50ng/5µL 11 30ng/5µL 12 0.64µg/5µL 2 (13~18) －20℃ : Store at ｰ20℃ Ladder for 10minute separation NO DISPLAY: Store at 2～10℃ DNA Step Ladder DNA Step Ladder DNA Step Ladder DNA Step Ladder Gene Ladder Fast 1 (10-200 bp) (50-1,500 bp) (100-5,000 bp) (500-10,000bp) (4 bands:0.1-2 kbp) Ready （bp） Ready （bp） Ready （bp） Ready （bp） Ready （bp）（ng/5µL） to to to to to use use use use use 10,000 －20℃ －20℃ －20℃ 5,000 －20℃ 4,000 2,000 25 200 2,000 2,000 1,000 25 100 1,500 1% Agarose S 500 25 6x 6x 6x 6x 1,000 100 25 Loading 50 Loading Loading Loading Dye Dye 850 Dye 850 Dye 20 500 17 400 400 Concentration: 10 0.1µg/5µL 200 100 Gene Ladder Fast 2 (6 bands:0.1-10 kbp) 4% Agarose 21 50 2% Agarose 2% Agarose 2% Agarose Ready （bp）（ng/5µL） to use Loading: Loading: Loading: Loading: 10,000 25 20µL/lane 20µL/lane 20µL/lane 20µL/lane 4,000 25 2,000 25 1,000 25 1% Agarose S 500 25 100 25 13 14 15 16 18 Concentration: 0.15µg/5µL Number Product nameCode No. Package Size Note 1 10 bp DNA Step Ladder (10-100 bp) 040-28721 32.5 μg (50 μL) 2 25 bp DNA Step Ladder (25-300 bp) 292-69001 100 μg 3 25 bp DNA Step Ladder (25-500 bp) 044-30711 500 μL 4 50 bp DNA Step Ladder (50-800 bp) 544-02311 85 μg (250 μL) 5 DNA Step Ladder（10-300 bp） 298-66301 50 μg 6 100 bp DNA Step Ladder（0.1-1.5 kbp） 296-68801 30 μg 319-06044 25 μL 7 OneSTEP Ladder 50（0.05-2.0 kbp） 315-06041 500 μL 311-06043 500 μL ×2 317-05244 25 μL 8 OneSTEP Ladder 100（0.1-2 kbp） 313-05241 500 μL 319-05243 500 μL ×2 317-05364 25 μL 9 OneSTEP Ladder 500 (0.5-5 kbp) 313-05361 500 μL 319-05363 500 μL × 3 314-05374 25 μL 10 OneSTEP Ladder 1 kb（1-10 kbp） 310-05371 500 μL 316-05373 500 μL × 3 11 OneSTEP Ladder Supercoiled Plasmid 317-05381 500 μL 12 Gene Ladder 100（0.1-2 kbp） 316-06951 500 μL 13 DNA Step Ladder（10-200 bp）, Fast Separation Type 292-66201 10 μg（0.5mL） × 2 14 DNA Step Ladder（50-1,500 bp）, Fast Separation Type 290-61001 10 μg（0.5mL） × 2 15 DNA Step Ladder（100-5,000 bp）, Fast Separation Type 296-61101 10 μg（0.5mL） × 2 16 DNA Step Ladder（500-10,000 bp）, Fast Separation Type 292-61201 10 μg（0.5mL） × 2 17 Gene Ladder Fast 1（4 bands:0.1-2 kbp） 317-06981 500 μＬ × 2 18 Gene Ladder Fast 2（6 bands:0.1-10 kbp） 314-06991 500 μＬ × 2 : Nippon Gene’s products 3 DNA Ladder DNA Ladder Wide Range Ladderder (19~2219~22) for wide-range and simultaneous analysislli i of ffb bboth thth DNA size i and d amount. t DNA Step Ladder Mix Smart Ladder Gene Ladder Wide 1 Gene Ladder Wide 2 (0.08-10 kbp) (0.2-10 kbp) (0.1-20 kbp) (0.1-20 kbp) （bp）（ng/20µL） Ready （bp）（ng/5µL） Ready （bp）（ng/5µL） Ready （bp）（ng/5µL） －20℃ to to to 10,000 200 use 10,000 100 use use 8,000 160 20,000 100 20,000 100 6,000 120 8,000 80 5,000 100 －20℃ 6,000 60 10,000 50 10,000 50 6x 4,000 80 5,000 50 7,000 35 7,000 35 Loading 3,000 60 Dye 4,000 40 5,000 75 5,000 75 2,500 50 4,000 40 4,000 40 2,000 40 3,000 30 2,500 25 3,000 30 3,000 30 1,500 32 2,000 20 1,500 15 2,000 100 2,000 100 1,030 200 1,000 100 1,500 30 1,500 30 900 180 800 80 1,300 26 1,000 50 900 45 800 160 600 60 1,000 100 700 140 800 40 600 120 400 40 700 35 700 35 600 30 500 200 200 20 500 75 400 500 75 400 80 40 400 40 1% Agarose S 300 30 300 60 200 20 200 20 200 40 100 100 100 100 1% Agarose 19 100 20 20 21 1% Agarose 22 1% Agarose 80 16 Ready to ( ) Marker － 20℃ & the OneSTEP Marker use 23~33 DNADNA momolecular-weightlec lar eight mmarkersarkers tthathat sesett sisizee sstandardstandards fforor llinearinear dodouble-strandble strand DNDNA.A EEach One-Step Marker comes with pre-added dye and glycerol, so they can be applied directly to the agarose gel. Marker 1 Marker 2 Marker 3 Marker 4 (λ/Hind Ⅲ) (λ/Hind Ⅲ・EcoRⅠ) (λ/Hind Ⅲ+λ/EcoRⅠ) (φX174/Hae Ⅲ) （kbp） （kbp） （kbp） （bp） 23.13 21.23 23.13 1,353 1,078 9.42 5.15 21.23 872 6.56 9.42 4.98 7.42 603 4.36 4.27 6.56 5.81 3.52 5.65 2.02 4.88 310 2.32 4.36 2.02 1.91 3.52 281 1.58 2.32 271 2.02 234 1.38 194 0.95 118 0.83 23 24 25 26 3% Agarose 21 72 1% Agarose S 1% Agarose S 1% Agarose S Marker 5 Marker 6 Marker 7GT Marker 8GT (φX174/HincⅡ) (λ/StyⅠ) (T4 GT7+T4 GT7/BglⅠ) (T4 GT7+T4 GT7/BglⅡ) （bp） （kbp） （kbp） （kbp） 1,057 19.33 165.65 165.65 770 50.31 56.74 7.74 41.77 612 6.22 495 23.45 17.82 392 4.26 21.10 345 3.47 17.70 17.70 341 15.71 2.69 335 14.28 10.65 297 10.06 291 1.88 210 8.03 162 1.49 5.59 0.93 5.51 27 79 28 29 30 3% Agarose 21 1% Agaose S 0.3% Agarose H 0.3% Agarose H 4 Marker 9 Marker 10 Marker 11 (φX174/HinfⅠ) (pBR322/MspⅠ) (pUC19/MspⅠ) （bp） （bp） （bp） 726 622 527 713 501 553 404 307 489 500 Storage conditions 427 242 404 417 238 331 DNA MW Marker ： 413 217 242 201 －20℃ 311 190 190 OneSTEP Marker： 249 180 147 200 160 111 Store at 2～10℃ 151 160 110 140 147 118 147 100 123 82 110 66 90 66 76 3% Agarose 21 3% Agarose 21 31 48 32 3% Agarose 21 67 33 Number Product nameCode No.
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