・DNA Step Ladders ・OneSTEP Ladders ・Ladders for 10minute separation ・Wide Range Ladders ・Markers ＆ OneSTEP Markers ・RRNANA SSizei SStandardt d Markers ・DGGE Markers ・RNA Step Ladder ・PCR Marker Makers ・Loading Buffers Molecular-Weight Markers

・EasyEasy SeSeparatorparatorTM ・Negative Gel Stain MS Kit ・SuperSepTM Ace ・Prestain markers ・SuperSepTM DNA ・ Western marker ・ Biotinylated marker ・ Markers for CBB dyeing

co.jp DNA Ladder DNA Ladder

(1~6) Ready With dye and glycerol already DNA Step Ladder to added, it is ready to be applied to use for low-molecular-range analysis. the agarose gel.

10 bp DNA Step 25 bp DNA Step 25 bp DNA Step 50 bp DNA Step Ladder (10-100 bp) Ladder (25-300 bp) Ladder (25-500 bp) Ladder (50-800 bp)

－20℃ （bp） －20℃ （bp） －20℃ （bp）（ng） －20℃ （bp） 500 60 100 300 800 400 60 90 275 700 300 60 600 6xBlue/ 80 6x 250 6xBlue/ Orange Loading 225 250 60 Orange 500 Loading 70 Dye Loading Dye 200 200 120 Dye 400 60 175 175 80 300 50 150 150 80 125 Loading: 40 Loading: Loading: 125 80 Loading: 200 30 100 100 120 1µL/lane 5µL/lane 5µL/lane 5µL/lane 150 20 75 75 80 10 50 50 80 100 25 25 80 50 1 2 3 4 3.5% Agarose 2% Agarose 2% Agarose 1% Agarose

OneSTEP Ladder (7~12) for simultaneous measurement of DNA size and amount.

DNA Step Ladder 100 bp DNA Step Ladder OneSTEP Ladder 50 OneSTEP Ladder 100 (10-300 bp) (0.1-1.5 kbp) (0.05-2 kbp) (0.1-2 kbp) （bp） （bp）（bp）（ng/5µL） （bp）（ng/5µL） Ready Ready Ready to 300 －20℃ 1,500 to 2,000 100 to 2,000 100 use use 1,500 75 use 1,800 90 200 1,000 1,000 50 900 1,600 80 －20℃ 900 45 1,400 70 150 6x 800 800 40 Loading 700 35 1,200 60 100 Dye 1,000 100 75 717 600 30 500 75 900 45 6x 50 600 Loading 400 22.5 800 40 Dye 35 500 450 20 700 35 350 17.5 600 30 25 400 300 15 20 250 37.5 500 50 15 286 200 10 400 20 10 150 7.5 300 15 200 100 10 200 10 100 10

100 50 100 2% Agarose 3% Agarose 21 3% Agarose 21 5% Agarose 21 Loading: Loading: Concentration: Concentration: 5 5-10µL/lane 6 5µL/lane 7 0.69µg/5µL 8 0.755µg/5µL

OneSTEP Ladder 500 OneSTEP Ladder 1 kb OneSTEP Ladder Gene Ladder 100 (0.5-5 kbp) (1-10 kbp) Supercoiled Plasmid (0.1-2 kbp)

Ready （bp） Ready （kbp） Ready （kbp） Ready （bp）（ng/5µL） to to to to use 5,000 use use use 4,500 10.0 2,000 100 4,000 9.0 10.0 8.0 －20℃ 1,500 75 3,500 9.0 3,000 7.0 8.0 1,000 50 2,500 6.0 7.0 900 45 2,000 5.0 6.0 4.0 800 40 5.0 700 35 1,500 3.0 4.0 600 30 2.0 500 75 1,000 3.0 400 40 300 30 200 20 500 1.0 100 100 1% Agarose S 2% Agarose S 1% Agarose S 1% Agarose S each fragment: each fragment: each fragment: Concentration: 9 50ng/5µL 10 50ng/5µL 11 30ng/5µL 12 0.64µg/5µL

2 Ready － 13 Loading ubr rdc aePcaeSz Note Package Size Productname Number use to 20℃ Ladder for10minuteseparation Dye 6x DNA StepLadder 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1

4% Agarose 21 (10-200 bp) 20µL/lane Loading: Gene LadderFast2 Gene LadderFast1 DNA StepLadder DNA StepLadder DNA StepLadder DNA StepLadder Gene Ladder100 OneSTEP LadderSupercoiledPlasmid OneSTEP Ladder1kb OneSTEP Ladder500(0.5-5kbp) OneSTEP Ladder100 OneSTEP Ladder50 100 bpDNAStepLadder DNA StepLadder 50 bpDNAStepLadder(50-800bp) 25 bpDNAStepLadder(25-500bp) 25 bpDNAStepLadder(25-300bp) 10 bpDNAStepLadder(10-100bp) （bp） 100 200 20 50 10 Ready － Loading 14 use to 20℃ Dye 6x DNA StepLadder （500-10,000 bp） （100-5,000 bp） （50-1,500 bp） （10-200 bp） （10-300 bp） （0.1-2 kbp） (50-1,500 bp) （4 bands:0.1-2kbp） （6 bands:0.1-10kbp） 20µL/lane 2% Agarose （0.05-2.0 kbp） Loading: （0.1-2 kbp） （1-10 kbp） （0.1-1.5 kbp） , FastSeparationType

1,500 （bp） , FastSeparationType 200 400 850 50 , FastSeparationType , FastSeparationType

Ready － Loading use 15 to 20℃ Dye 6x DNA StepLadder (100-5,000 bp) 2% Agarose 20µL/lane Loading: 3 （bp） 2,000 5,000 ( 100 400 850 13~18 Ready － Loading use to 20℃ 16 314-06991 317-06981 292-61201 296-61101 290-61001 292-66201 316-06951 317-05381 316-05373 310-05371 314-05374 319-05363 313-05361 317-05364 319-05243 313-05241 317-05244 311-06043 315-06041 319-06044 296-68801 298-66301 544-02311 044-30711 292-69001 040-28721 Dye 6x DNA StepLadder Code No. ) (500-10,000bp)

20µL/lane 2% Agarose Loading: 10,000 （bp） 1,000 2,000 4,000 500 25 μL 25 μL 25 μL 25 μL 30 μg 50 μg 85 μg (250 μL) 500 μL 100 μg 32.5 μg (50 μL) 500 μ 500 μ 500 μL 500 μL 500 μL ×3 500 μL 500 μL ×3 500 μL 500 μL ×2 500 μL 500 μL ×2 500 μL 10 μg 10 μg 10 μg 10 μg Ready Ready 18 17 2 × Ｌ 2 × Ｌ use use to to : NO DISPLAY: （0.5mL） （0.5mL） （0.5mL） （0.5mL） (6 bands:0.1-10kbp) NipponGene’sproducts － Gene LadderFast1 Gene LadderFast2 (4 bands:0.1-2kbp) 20℃

Concentration: 1% Agarose S Concentration:

0.15µg/5µL 1% Agarose S 0.1µg/5µL × 2 × × 2 × × 2 × × 2 × : Store at2～10℃ Store atｰ20℃ 10,000 （bp） （bp） 1,000 2,000 4,000 1,000 2,000 100 25 500 25 0 25 100 25 500 （ng/5µL） （ng/5µL） 25 25 25 25 25 25 DNA Ladder DNA Ladder Wide Range Ladderder (119~229~22) for wide-range and simultaneous analysislli i of ffb bbotht thh DNA size i and d amount. t

DNA Step Ladder Mix Smart Ladder Gene Ladder Wide 1 Gene Ladder Wide 2 (0.08-10 kbp) (0.2-10 kbp) (0.1-20 kbp) (0.1-20 kbp)

（bp）（ng/20µL） Ready （bp）（ng/5µL） Ready （bp）（ng/5µL） Ready （bp）（ng/5µL） －20℃ to to to 10,000 200 use 10,000 100 use use 8,000 160 20,000 100 20,000 100 6,000 120 8,000 80 5,000 100 －20℃ 6,000 60 10,000 50 10,000 50 6x 4,000 80 5,000 50 7,000 35 7,000 35 Loading 3,000 60 Dye 4,000 40 5,000 75 5,000 75 2,500 50 4,000 40 4,000 40 2,000 40 3,000 30 2,500 25 3,000 30 3,000 30 1,500 32 2,000 20 1,500 15 2,000 100 2,000 100 1,030 200 1,000 100 1,500 30 1,500 30 900 180 800 80 1,300 26 1,000 50 900 45 800 160 600 60 1,000 100 700 140 800 40 600 120 400 40 700 35 700 35 600 30 500 200 200 20 500 75 400 500 75 400 80 40 400 40 1% Agarose S 300 30 300 60 200 20 200 20 200 40 100 100 100 100 1% Agarose 19 100 20 20 21 1% Agarose 22 1% Agarose 80 16

Ready to ( ) Marker － 20℃ & the OneSTEP Marker use 23~33 DNADNA momolecular-weightlec lar eight mmarkersarkers tthathat sesett sisizee sstandardstandards fforor llinearinear dodouble-strandble strand DNDNA.A EEach One-Step Marker comes with pre-added dye and glycerol, so they can be applied directly to the agarose gel.

Marker 1 Marker 2 Marker 3 Marker 4 (λ/Hind Ⅲ) (λ/Hind Ⅲ・EcoRⅠ) (λ/Hind Ⅲ+λ/EcoRⅠ) (φX174/Hae Ⅲ)

（kbp） （kbp） （kbp） （bp） 23.13 21.23 23.13 1,353 1,078 9.42 5.15 21.23 872 6.56 9.42 4.98 7.42 603 4.36 4.27 6.56 5.81 3.52 5.65 2.02 4.88 310 2.32 4.36 2.02 1.91 3.52 281 1.58 2.32 271 2.02 234 1.38 194 0.95 118 0.83

23 24 25 26 3% Agarose 21 72 1% Agarose S 1% Agarose S 1% Agarose S

Marker 5 Marker 6 Marker 7GT Marker 8GT (φX174/HincⅡ) (λ/StyⅠ) (T4 GT7+T4 GT7/BglⅠ) (T4 GT7+T4 GT7/BglⅡ)

（bp） （kbp） （kbp） （kbp） 1,057 19.33 165.65 165.65 770 50.31 56.74 7.74 41.77 612 6.22 495 23.45 17.82 392 4.26 21.10 345 3.47 17.70 17.70 341 15.71 2.69 335 14.28 10.65 297 10.06 291 1.88 210 8.03 162 1.49 5.59 0.93 5.51 27 79 28 29 30 3% Agarose 21 1% Agaose S 0.3% Agarose H 0.3% Agarose H

4 31 ubr rdc aePcaeSz Note Package Size Productname Number 22 21 20 19 33 32 31 30 29 28 27 26 25 24 23 ( φ X174/ 3% Agarose 21 Marker 9 Hin （lambda/Hind Ⅲ +lambda/EcoRIdigestmixture） （lambda/Hind Ⅲ +lambda/EcoRIdigestmixture） （lambda/Hind Ⅲ +EcoRIdoubledigest） （lambda/Hind Ⅲ +EcoRIdoubledigest） Marker 11 OneSTEP Marker11 Marker 10 OneSTEP Marker10 Gene LadderWide2 Gene LadderWide1 Smart Ladder(0.2-10kbp) DNA StepLadderMix Marker 9 OneSTEP Marker1 OneSTEP Marker9 Marker 8GT Marker 7GT Marker 6 OneSTEP Marker6 Marker 5 OneSTEP Marker5 Marker 4 OneSTEP Marker4 Marker 3 OneSTEP Marker3 Marker 2 OneSTEP Marker2 Marker 1 f Ⅰ ) （bp） 100 118 140 151 200 249 311 413 417 427 500 553 713 726 48 66 66 82 （lambda/Sty （lambda/Hind Ⅲ digest） （phiX174/Hinf （phiX174/Hinc Ⅱ digest） （phiX174/Hae Ⅲ digest） （pUC19/Msp （pBR322/Msp 32 （phiX174/Hinf （lambda/Sty （phiX174/Hinc Ⅱ digest） （phiX174/Hae Ⅲ digest） （lambda/Hind Ⅲ digest） （0.1-20 kbp） （0.1-20 kbp） （pUC19/Msp （pBR322/Msp （0.08-10 kbp） Ⅰ digest） (pBR322/ Ⅰ Ⅰ digest） digest） Ⅰ digest） 3% Agarose 21 Marker 10

Ⅰ

digest） Msp

Ⅰ

Ⅰ

digest） digest） Ⅰ

digest） Ⅰ

)

b）（bp） （bp） 110 123 147 147 160 160 180 190 201 217 238 242 307 404 527 622 67 76 90

5 33 (pUC19/ Marker 11 315-03263 319-03261 318-05833 312-05831 313-01503 317-01501 311-05823 315-05821 310-01493 314-01491 314-05813 318-05811 318-03993 312-03991 311-03983 315-03981 319-00966 313-00964 317-05283 311-05281 318-00676 312-00674 317-05803 311-05801 311-00666 315-00664 314-05793 318-05791 312-00576 316-00574 310-05273 314-05271 315-00566 319-00564 311-05264 317-05261 312-00456 316-00454 314-05254 310-05251 310-06971 313-06961 317-03941 313-03943 292-68401 3% Agarose 21 Code No. Msp Ⅰ ) 110 111 147 190 242 331 404 489 501 1500 μL 1500 μL 1500 μL 1500 μL 1500 μL ×5 1500 μL ×5 1500 μL ×5 1500 μL ×5 120 μg 120 μg ×5 80 μg 500 μL 375 μL 375 μL 375 μL 375 μL 375 μL 500 μL ×2 500 μL ×2 375 μL ×5 375 μL ×5 375 μL ×5 375 μL ×5 375 μL ×5 80 μg ×5 80 μg ×5 80 μg ×5 15 μg 15 μg 15 μg 10 μg 10 μg 80 μg 15 μg 15 μg 80 μg 15 μg ×5 15 μg ×5 15 μg ×5 10 μg ×5 10 μg ×5 15 μg ×5 15 μg ×5 25 μL Store at2～10℃ OneSTEP Marker： DNA MWMarker ： 1 mL Storage conditions － 20℃ : NipponGene’sproducts DNA Ladder DNA Ladder DGGE Marker (34~384~38) DNADNA mamarkersrkers foforr DGGE ((denaturingdenaaturingaturing g gradientrad gel electrophoresis)electrophoresis). A CG clamp is aadded at the end of one side of each fragment to enable separation/inspection by DGGE.

DGGE MarkerⅠ DGGE Marker Ⅱ DGGE Marker Ⅲ DGGE Marker Ⅳ DGGE Marker Ⅴ (5 fragments) (10 fragments) (10 fragments) (8 fragments) (9 fragments)

Denaturant Denaturant Denaturant Denaturant Denaturant 35% 35% 50% 20% 20% 1 1 1 －20℃ －20℃ 2 －20℃ －20℃ －20℃ 2 2 3 1 4 2 3 3 1 3 5 2 4 4 3 5 6 5 4 6 4 7 6 5 7 7 8 8 6 8 5 9 9 7 9 8 10 10

34 8% Acrylamide 35 36 37 38 8% Acrylamide 7% Acrylamide 6% Acrylamide 65% 65% 70% 6% Acrylamide 45% 50%

No. of fragments (Conc.) 5 (50ng/5µL) 10 (100ng/5µL) 10 (100ng/5µL) 8 (80ng/5µL) 9 (50ng/5µL) Application For bacterial 16S rDNA For bacterial 16S rDNA For bacterial 16S rDNA For fungal bacteria16S rDNA For fungal bacteria 16S rDNA Constant voltage 120V 120V 50V 50V 75V Electrophoresis time, Temp. 8 hours, 60℃ 8 hours, 60℃ 18 hours, 58℃ 20 hours, 60℃ 16 hours, 60℃

PCR Marker Maker (39~42) －20℃ for preparing DNA size markersmarkers. Consisting of a mixtmixture of template DNA, primer, and reaction buffer, the reagent initiates a PCR with the simple addition of a Tag DNA polymerase “Gene Tag.” It can also be used as a positive control while the PCR is under way. M 39 40 41 42 43 （bp） （bp） 1057 M：Marker 5（φX174/ Hinc Ⅱ） 0.5 µg 770 39：100L（101 bp, 200 bp, 299 bp, 398 bp, 502 bp） 3 µL 1005 612 3 µL 905 40：100H（601 bp, 702 bp, 800 bp, 906 bp, 1,005 bp） 495 800 41：200U（101 bp, 299 bp, 502 bp, 702 bp, 906 bp） 3 µL 392 702 42：200E（200bp, 398 bp, 601 bp, 805 bp, 1,005 bp） 3 µL 345 601 3 µL each 341 43：Mixture of products from a 100L & 100H PCR 502 335 398 297 299 291 200 210 101 162 79

Number Product nameCode No. Package Size Note 318-06411 30 μL（for 6 applications） 34 DGGE Marker Ⅰ (5 fragments) 314-06413 150 μL（for 30 applications） 312-06414 150 μL × 5（for 150 applications） 311-06401 30 μL（for 6 applications） 35 DGGE Marker Ⅱ (10 fragments) 317-06403 150 μL（for 30 applications） 315-06404 150 μL × 5（for 150 applications） 315-06921 30 μL（for 6 applications） 36 DGGE Marker Ⅲ (10 fragments) 311-06923 150 μL（for 30 applications） 319-06924 150 μL × 5（for 150 applications） 312-06931 30 μL（for 6 applications） 37 DGGE Marker Ⅳ (8 fragments) 318-06933 150 μL（for 30 applications） 316-06934 150 μL × 5（for 150 applications） 319-06941 30 μL（for 6 applications） 38 DGGE Marker Ⅴ (9 fragments) 315-06943 150 μL（for 30 applications） 313-06944 150 μL × 5（for 150 applications） PCR Marker Maker 100L 317-03061 For 10 applications 39 PCR Marker Maker 100L, Gene Taq attached 314-03071 For 10 applications PCR Marker Maker 100H 311-03081 For 10 applications 40 PCR Marker Maker 100H, Gene Taq attached 318-03091 For 10 applications PCR Marker Maker 200U 318-03111 For 10 applications 41 PCR Marker Maker 200U, Gene Taq attached 315-03121 For 10 applications PCR Marker Maker 200E 312-03131 For 10 applications 42 PCR Marker Maker 200E, Gene Taq attached 319-03141 For 10 applications

: Nippon Gene’s products 6 RNA Ladder RNA Ladder RNA Size Standard Marker (1~4) RNA molecular-weight markers prepared by in vitro transcription.

RNA Size Standard RNA Size Standard RNA Size Standard RNA Ladder Marker Ⅱ Marker Ⅲ (0.1-1 kb) Marker Ⅳ (0.28-6.58 kb) (0.125-6 kb)

－70℃ （k base）－70℃ （base） －80℃ （base） －70℃ （base） 9 1,000 6,583 750 6,000 4,981 6 500 4,000 5 400 3,638 3,000 2,000 2,604 4 300 1,500 1,908 1,000 3 1,383 500 2.5 200 250 2 955 125 1.5 623 1 1% formaldehyde Agarose 281 1 0.5 2 100 3 4 5% polyacrylamide-urea 1% formaldehyde Agarose 1% formaldehyde Agarose

(5) RNA Step Ladder (0.1–1 kb) Loading Buffer（Store at room temp.） an RNARNA molecular-weightl l i ht markerk can bbe didirectlytl appliedli d A sample can be easily applied during DNA gel to native gel, denatured gel, or polyacrylamide gel. electrophoresis to effectively predict the migration point.

abcdefa bcdef RNA Step Ladder (0.1-1 kb)

Xylene Cyanol FF －80℃ （base） （base）

Bromophenol Blue 2X 1,000 1,000 Loading 800 Dye 800 Orange G 600 600 400 400 300 a Marker 4 6 × Loading Buffer Triple Dye 300 b (φX173 / H a e Ⅲ) 6 × Loading Buffer Orange G 200 200 c Product PCR 6 × Loading Buffer Triple Dye d (299 bp) 50 ng 6 × Loading Buffer Orange G e 6 × Loading Buffer Triple Dye 100 100 TE Buffer f 6 × Loading Buffer Orange G Orange G Bromophenol Blue Xylene Cyanol FF 1 % agarose S 100 bp (approx.) 900 bp (approx.) 5,000 bp (approx.) 2% Native Agarose 5 2% Formaldehyde Agarose 2 % agarose S 30 bp (approx.) 300 bp (approx.) 1,500 bp (approx.) 3 % agarose 21 10 bp (approx.) 100 bp (approx.) 500 bp (approx.)

Number Product nameCode No. Package Size Note 1 RNA Size Standard Marker Ⅱ（0.5-9 kb） 542-00651 50 μg 2 RNA Size Standard Marker Ⅲ（0.1-1 kb） 545-01621 50 μg 3 RNA Size Standard Marker Ⅳ（0.28-6.58 kb） 188-01831 50 μL 311-06261 25 μL 4 RNA Ladder（0.125-6 kb） 317-06263 25 μL × 2 5 RNA Step Ladder（0.1-1 kb） 298-61301 40 μL × 5 － Loading Buffer 313-90111 10 mL － 6 × Loading Buffer OrangeG 317-90251 1 mL × 3 － 6 × Loading Buffer Double Dye 313-90351 1 mL × 3 － 6 × Loading Buffer Triple Dye 314-90261 1 mL × 3

: Nippon Gene’s products 7 Protein Size Marker Protein Size Marker Electrophoresis-related Products

Prestained Marker (1, 2) : With dye combined in its recombinant protein content. (3) Contains protein G with the ability to combine with the Fc region Western Marker : of immunoglobulin. Reacts with both primary and secondary antibodies in a western blot.

(4) Contains biotinylated protein. Capable of both chromogenetic and Biotinylated Marker : photogenetic detection by reacting with avidin HRP or avidin AP.

－20℃ （kDa） －20℃ （kDa） －20℃ （kDa） （kDa） 240 140 140 150 79 120 100 100 100 70 80 70 42 60 50 50 50 40 35 42 31 35 30 25 20 25 21 20 15 14 15 16 7 1 Prestained 2 PrestainedⅡ 3 Western 4 Biotinylated

Marker for CBB Dyeing (5~8) Optimized for CBB dyeing. Obtains sharp, uniform bands because of a reduction alkyl process.

（kDa） （kDa） （kDa） （kDa）

200 180 116 97 116 79 79 79

42 42 42 42 30 30 30 30

20 20 20 17 14 17 14 6.5 6.5 3.5

For low molecular For middle molecular For high molecular Wide range 5 range 6 range 7 range 8

－20℃ : Store at ｰ20℃ NO DISPLAY: Store at 2～10℃

Number Product name Wako Cat. No. Package Size 1 WIDE-VIEW TM Prestained Protein Size Marker 230-02221 500 μL 2 WIDE-VIEW TM Prestained Protein Size Marker 2 239-02291 500 μL 3 WIDE-VIEW TM Western Size Marker 233-02211 250 μL 4 WIDE-VIEW TM Biotinylated Size Marker 233-02331 1.5 mL 5 Molecular Weight Marker, Low Range 294-63101 1 mL 6 Molecular Weight Marker, Middle Range 131-14511 1 mL 7 Molecular Weight Marker, High Range 134-14501 1 mL 8 Molecular Weight Marker, Wide Range 296-63301 1 mL

8 EElectrophoresis-relatedlectrophoresis-related PProductsroducts Electrophoresis-related Products

TM Easy Separator (Wako Catalog No.058-07681 (1 set)) Easy SeparatorTM is an electrophoresis tank specially designed for “SuperSep” products to enable easy setting and removal of the gel plate. Features

1 Easy to operate. 2 Requires only a small amount (about 250-350ml) of running buffer for electrophoresis. 3 Allows the gel plate to be removed without removing the running buffer after electrophoresis has been completed.

Easy Set Easy Remove

①Insert a gel. ②Turn the knobs ③Setting is ①Remove the cover ②Turn the knobs ③Remove the gel toward you. completed. after electrophoresis away from you. without removing has been completed. the running buffer.

Negative Gel Stain MS Kit （Store at room temperature） Protein bands separated by SDS/polyacrylamide gel electrophoresis (SDS-PAGE) can be visualized as stable, clear and transparent bands against the background of milky white gel stained by negative gel stain containing our improved imidazol derivative reagent in as little as 10 minutes. The staining technique is useful to obtain the clear and sensitive resolution pattern of the gel before immunoblotting as well as to excise and purify the band of interest from the gel without significant deterioration of amino acid residues for the subsequent studies of protein such as sequencing and mass analysis of peptide.

Procedure Featureas

SDS-PAGE gel 1 Detectable limit of protein: 1 ng Staining Sol. A 2 Sensitive and Simple procedure with 2-step staining in Shake 5 ~ 10 min. 5~10 minutes Wash 5 ~ 10 sec. x 3 3 Optimized the staining intensity by either de-staining the Staining Sol. B excess stain or re-staining following de-stain of the gel. Shake 10 ~ 60 sec. Wash 1 min. x 3 4 Applicable to proteome analysis by mass spectrometry and sequencing

Check staining intensity of the gel against a sheet Mass Spectrometry of black paper MALDI-TOF/MS analysis rat muscle glycogen phosphorylase (EC2.4.1.1) SDS-PAGE analysis 123

a Figure 1. b SDS-PAGE analysis of proteins using

relative abundance Figure 2. Negative Gel Stain MS Kit c MALDI-TOF/MS of a : rat phosphorylase (97k); 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 rat phosphorylase b : bovine serum albumin (68k); m/z d c : hen egg white ovalbumin (45k); The band was excised and treated with trypsin. Following the in-gel digestion and d : bovine carbonic anhydrase (31k). preparation, the sample was analyzed on MALDI-TOF mass spectrometer. (These data Lane 1: 50 ng; Lane 2: 100 ng; Lane 3: 150 ng were provided by Dr. Y. Wada at Osaka Medical Center, Japan.)

Product name Wako Cat. No. Package Size Negative Gel Stain MS Kit Kit Contents: 293-57701 20 tests 1) Staining Solution A (1 × 500 mL); 2) Staining Solution B (1 × 500 mL); 3)De-staining Solution (1 × 500 mL)

9 EElectrophoresis-relatedlectrophoresis-related PProductsroducts Electrophoresis-related Products SuperSepSuperSepTM AAcece SuperSep™SuperSep™ AcAcee iiss a popolyacrylamidelyacrylaamide ggelel ooff ththee hihighestghest ququality,ality 1313 we wellll 1717 we wellll designed to resolve the problems of existing products (SuperSep™ and SuperSep™ HG). Tris-glycine buffer is used in the gel buffer, similar to existing products, but because its pH is neutralized, the gel’s chemical storage stability is improved. Physical storage stability is also improved by using vacuum packaging. And because it does not contain SDS, it can be used in SDS-PAGE and Native-PAGE by changing the running buffer. nient onve r CConvenienter fo stickerstick for gelgel ion! Features ificat iidentification!dent 1 Good separation and uniform bands 2 Can be used with a multi-channel pipette. 3 Reasonably priced and capable of long-term storage (for 6 months) 4 Box sticker allows easy identification of type of gel. 5 Comes in 13-well and 17-well types.

oved IImprovedmpr n ratio separationsepa ter! Product specifications e cen r th TM nearnea the center! TM SuperSep Plate size 100 × 100 × 3（ mm） SuperSep Ace Gel size 90 × 85 × 1 mm （ ） （kDa） Number of wells 13 wells 17 wells 200 Well volume 30 μL 25 μL 150 100 ＊ 3.3 6.5 μg 1.3 3.9 μg Total amount of protein (/well) ～ ～ 75 ＊Indicates total amount of protein that can be clearly separated.

50

37 Uniform ① SuperSepTM: 10% band! 0 1.5 3 4.5 6 7.5 9 10.5 12 (hours)

25

TM ②SuperSep Ace: 10% 20

（Store at 2～10℃） Product name Wako Cat. No. Package Size SuperSepTMAce, 6%, 13well 195-15171 10 ea. SuperSepTMAce, 6%, 17well 192-15181 10 ea. SuperSepTMAce, 7.5%, 13well 198-14941 10 ea. SuperSepTMAce, 7.5%, 17well 191-14931 10 ea. SuperSepTMAce, 10%, 13well 195-14951 10 ea. SuperSepTMAce, 10%, 17well 192-14961 10 ea. SuperSepTMAce, 12.5%, 13well 199-14971 10 ea. SuperSepTMAce, 12.5%, 17well 196-14981 10 ea. SuperSepTMAce, 15%, 13well 193-14991 10 ea. SuperSepTMAce, 15%, 17well 190-15001 10 ea. SuperSepTMAce, 5-12%, 13well 199-15191 10 ea. SuperSepTMAce, 5-12%, 17well 192-15201 10 ea. SuperSepTMAce, 5-20%, 13well 197-15011 10 ea. SuperSepTMAce, 5-20%, 17well 194-15021 10 ea. SuperSepTMAce, 10-20%, 13well 191-15031 10 ea. SuperSepTMAce, 10-20%, 17well 198-15041 10 ea. SuperSepTMAce, 15-20%, 13well（Tricine Gel） 198-15301 10 ea.

10 Electrophoresis-related Products TM SuperSep DNA (15%, 17 wells) (Wako Catalog No.190-15481 (10 ea.))（Store at 2～10℃） Polyacrylamide gel is used in the separation of low-molecular-weight DNA because it has a finer gel mesh structure than agarose. However, the DNA size will vary widely depending on the acrylamide concentration and the degree of cross-linkage of the gel. In SuperSepTM DNA, the acrylamide concentration and the degree of cross-linkage of the gel have been optimized for the separation of low-molecular-weight DNA. This product is therefore effective for the separation of samples with a DNA size from 20-200 bp. t Features Product specifications enien Conv r 100 × 100 × 3 mm er fo 1 The acrylamide concentration, degree of Plate size （ ） stick gel cross-linkage, and number of wells are optimized for Gel size 90 × 85 × 1（ mm） ion! ificat the electrophoresis of low-molecular-weight DNA. ident Number of wells 17 wells 2 Cleaner separation image than that obtained with Well volume 25 μL agarose gel. 3 Ideal for 20-200 bp separation.

（bp） （bp） （bp） （bp）

100 90 oved 80 mpr 200 I tion 70 200 epara er! s cent 60 150 150 the 100 ear 50 n 90 40 100 100 80 30 70 75 75 20 60 50 50 50 40 25

30 25 20

TM TM 1% Agarose SuperSep DNA 2.5% Agarose SuperSep DNA （15%, 17 well） （15%, 17 well）

Example of electrophoresis 20-200 bp DNA was clearly separated by using SuperSepTM DNA and Tris-glycine buffer. PCR products under 200 bp can be used for separation from gel, especially when cloning small RNA. 30-200 bp DNA was clearly separated with TBE buffer.

① With Tris-Glycine running buffer ② With TBE running buffer （bp） （bp） （bp） （bp）

200 150 200 100 150 100 50 50 30 25 30 20 20 25 EtBr staining EtBr staining

Staining Reagent

Product name Wako Cat. No. Package Size EtBr Solution 315-90051 10 mL

11 Selection Guide 【How to read the chart】【】 【Display codes】 ●1 , p1 NO DISPLAY Store at 2～10℃ －80℃ Store at or below ｰ80℃ Number Reference page －20℃ Store at or below ｰ20℃ －70℃ Store at or below ｰ70℃ DNA Ladders 10 bp 100 bp 1 kbp 10 kbp 100 kbp 200 kbp

●1 , p2 ●2 , p2 ●3 , p2 ●4 , p2 ●5 , p2 ●6 , p2 ●7 , p2 ●8 , p2 ●9 , p2 ●10 , p2 ●11 , p2 ●12 , p2 ●13 , p3 ●14 , p3 ●15 , p3 ●16 , p3 ●17 , p3 ●18 , p3 ●19 , p4 ●20 , p4 ●21 , p4 ●22 , p4 ●23 , p4 ●24 , p4 ●25 , p4 ●26 , p4 ●27 , p4 ●28 , p4 ●29 , p4 ●30 , p4 ●31 , p5 ●32 , p5 ●33 , p5 ●39 , p6 ●40 , p6 ●41 , p6 ●42 , p6

10 bp 100 bp 1 kbp 10 kbp 100 kbp 200 kbp RNA Ladders 10 b 100 b 1000 b 10000 b

●1 , p7 ●2 , p7 ●3 , p7 ●4 , p7 ●5 , p7

10 b 100 b 1000 b 10000 b Protein Size Markers 10 kDa 100 kDa 1000 kDa

●1 , p8 ●2 , p8 ●3 , p8 ●4 , p8 ●5 , p8 ●6 , p8 ●7 , p8 ●8 , p8

10 kDa 100 kDa 1000 kDa

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