For life science research only. Not for use in diagnostic procedures. DNA Molecular Weight Marker XIV (100–1500 bp) 100 base pair ladder

Cat. No. 11 721 933 001 y Version 08 ␮ c 50 g 1 A260 unit Content version: October 2018 50 ␮g (200 ␮l) for 50 gel lanes Store at Ϫ15 to Ϫ25°C

Product overview

Formulation Solution in 10 mM Tris-HCl, 1 mM EDTA, pH 8

Concentration 250 µg/ml

Size distribution Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases. The mixture contains 15 double stranded DNA frag- ments with the following base pair lengths: 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500 and an additional band of 2642 bp. The 500 bp and 1000 bp banding pattern is two to three times brighter.

Application The DNA Molecular Weight Marker XIV allows accurate sizing of DNA fragments generated by PCR or restric- tion digest separated on agarose gels. The DNA molec- ular weight marker can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder)* for precise size determination. Electrophoretic separation of this molecular weight marker results in a regular pattern. The fragments have 5'-protruding ends which can be labeled by filling in reactions using e.g. [32P]-dTTP and [32P]-dGTP, or using DIG-11-ddUTP* and Terminal Transferase*.

Typical analysis The DNA fragment mixture shows the ladder with 15 bands and an additional band of 2642 bp in agarose gel electrophoresis as shown in the figure. Fig.: Separation of 1 µg DNA molecular weight marker XIV on a 2% Separation Apply 1 µg DNA per lane on a 2% Agarose MP* gel. agarose gel. Ethidiumbromide stain. conditions

Storage/stability The unopened vial is stable at Ϫ15 to Ϫ25°C until the expiration date printed on the label Note: This product is shipped in dry ice and should be * available from Roche Diagnostics stored at Ϫ15 to Ϫ25°C until use.

Once thawed we recommend further storage at +2 to + 8°C. Repeated freezing and thawing should be avoided.

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Regulatory For life science research only. Not for use in diagnostic Contact and Support Disclaimer procedures. To ask questions, solve problems, suggest enhancements and report new applications, please visit our Online Technical Support Site. Disclaimer of For patent license limitations for individual products To call, write, fax, or email us, visit sigma-aldrich.com, and select your home License please refer to: List of biochemical reagent products country. Country-specific contact information will be displayed.

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