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DNA Molecular Weight Marker VIII (19 – 1114 Bp) Pucbm21 DNA × Hpa II* Digested Pucbm21 DNA × Dra I* + Hind III* Digested

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DNA Molecular Weight Marker VIII (19 – 1114 Bp) Pucbm21 DNA × Hpa II* Digested Pucbm21 DNA × Dra I* + Hind III* Digested For life science research only. Not for use in diagnostic procedures. DNA Molecular Weight Marker VIII (19 – 1114 bp) pUCBM21 DNA × Hpa II* digested pUCBM21 DNA × Dra I* + Hind III* digested ־ ␮ Cat. No. 11 336 045 001 50 g 1 A260 unit y Version 19 50 ␮g (200 ␮l) for 50 gel lanes Content version: September 2018 Store at Ϫ15 to Ϫ25°C 1. Product Overview Content Ready-to-use solution in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 Concentration 250 ␮g/ml Size Distribution Fragment mixture prepared by cleavage of pUCBM21 DNA with restriction endonucleases Hpa II * and Dra I* plus Hind III*. The mixture contains 18 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 1114, 900, 692, 501, 489, 404, 320, 242, 190, 147, 124, 110, 67, 37, 34, (2×), 26, 19 (determined by computer analysis of the pUCBM21 sequence). Application Molecular weight marker for the size determination of DNA in agarose gels. Properties After gel electrophoresis of 1 ␮g of the fragment mixture in a 2% Aga- rose MP* gel 13 bands are visible. The 501 bp and 489 bp fragments as well as the fragments 37–19 bp run as one band. Typical Analysis Fig. 1: Separation of 1 ␮g DNA Molecular Weight Marker VIII on a The DNA fragment mixture shows the typical pattern of 13 bands in 1.8% agarose gel. Ethidiumbromide stain. agarose gel electrophoresis.. 2. Supplementary Information Storage/Stability The unopened vial is stable at Ϫ15 to Ϫ25°C until the expiration date Changes to Previous Version printed on the label. Editorial changes. This product is shipped on dry ice. Store at Ϫ15 to Ϫ25°C until use. Text Conventions Once thawed we recommend further storage at +2 to + 8°C. To make information consistent and understandable, the following text Avoid repeated thawing and freezing. conventions are used in this Instruction Manual: Text Convention Use Asterisk * Denotes a product available from Roche Diagnostics. Symbols Symbols are used in this Instruction Manual to highlight important information: Symbol Description Information Note: Additional information about the current topic or proce- dure. 0918.113527680019 sigma-aldrich.com Ordering Information Trademarks SURE/CUT and HIGH PURE are a trademarks of Roche. Product Application Packsize Cat. No. All third party product names and trademarks are the property of Rapid DNA Ligation of sticky- Kit (40 DNA 11 635 379 001 their respective owners. Ligation Kit end or blunt-end ligations) DNA fragments in Regulatory Disclaimer just 5 min at 15 – 25°C. For life science research only. Not for use in diagnostic procedures. T4 DNA Ligase Ligation of sticky- 100 U 10 481 220 001 and blunt ended 500 units 10 716 359 001 Disclaimer of License DNA fragments. (1 U/␮l) For patent license limitations for individual products please refer to: rAPid Alkaline 1,000 U 04 898 133 001 List of biochemical reagent products phosphatase Alkaline Phos- Dephosphorylation 1,000 U 11 097 075 001 phatase (AP), of 5´-phosphate (20 U/␮l) special quality residues from for molecular nucleic acids. biology Agarose MP Multipurpose 100 g 11 388 983 001 agarose for analyti- 500 g 11 388 991 001 cal and preparative electrophoresis of nucleic acids Agarose LM- Low melting point 50 g 11 441 345 001 MP agarose allows 100 g 11 441 353 001 enzymatic manipu- lations Agarose Gel For the elution of 1 Kit (max. 100 11 696 505 001 DNA Extraction DNA fragments reactions) Kit from agarose gels. High Pure PCR Purification of PCR 50 purifications 11 732 668 001 Product Purifi- or enzymatic modifi-250 purifications 11 732 676 001 cation Kit cation reaction (e.g. restriction digest) SuRE/Cut Buf- Incubation buffers 1 ml each (10× 11 082 035 001 fer Set for A,B,L,M and H for conc. solutions) Restriction restriction enzymes Enzymes SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 959 001 fer A incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 967 001 fer B incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 991 001 fer H incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 975 001 fer L incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 983 001 fer M incubation conc. solution) Water, PCR Specially purified, 100 ml 03 315 843 001 Grade double-distilled, (4 vials of 25 ml) deionized, and auto-25 ml 03 315 932 001 claved (25 vials of 1 ml) 25 ml 03 315 959 001 (1 vial of 25 ml) BSA, special Maintaining 20 mg (1 ml) 10 711 454 001 quality for enzyme stability molecular biology Contact and Support To ask questions, solve problems, suggest enhancements and report new applications, please visit our Online Technical Support Site. To call, write, fax, or email us, visit sigma-aldrich.com, and select your home country. Country-specific contact information will be displayed. Roche Diagnostics GmbH Sandhofer Strasse 116 68305 Mannheim Germany.
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