For life science research only. Not for use in diagnostic procedures.

DNA Molecular Weight Marker VIII (19 – 1114 bp) pUCBM21 DNA × Hpa II* digested pUCBM21 DNA × Dra I* + Hind III* digested

־ ␮ Cat. No. 11 336 045 001 50 g 1 A260 unit y Version 19 50 ␮g (200 ␮l) for 50 gel lanes Content version: September 2018 Store at Ϫ15 to Ϫ25°C

1. Product Overview

Content Ready-to-use solution in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0

Concentration 250 ␮g/ml

Size Distribution Fragment mixture prepared by cleavage of pUCBM21 DNA with restriction endonucleases Hpa II * and Dra I* plus Hind III*. The mixture contains 18 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 1114, 900, 692, 501, 489, 404, 320, 242, 190, 147, 124, 110, 67, 37, 34, (2×), 26, 19 (determined by computer analysis of the pUCBM21 sequence).

Application Molecular weight marker for the size determination of DNA in agarose gels.

Properties After gel electrophoresis of 1 ␮g of the fragment mixture in a 2% Aga- rose MP* gel 13 bands are visible. The 501 bp and 489 bp fragments as well as the fragments 37–19 bp run as one band.

Typical Analysis Fig. 1: Separation of 1 ␮g DNA Molecular Weight Marker VIII on a The DNA fragment mixture shows the typical pattern of 13 bands in 1.8% agarose gel. Ethidiumbromide stain. agarose gel electrophoresis.. 2. Supplementary Information Storage/Stability The unopened vial is stable at Ϫ15 to Ϫ25°C until the expiration date Changes to Previous Version printed on the label. Editorial changes.  This product is shipped on dry ice. Store at Ϫ15 to Ϫ25°C until use. Text Conventions Once thawed we recommend further storage at +2 to + 8°C. To make information consistent and understandable, the following text  Avoid repeated thawing and freezing. conventions are used in this Instruction Manual:

Text Convention Use Asterisk * Denotes a product available from Roche Diagnostics.

Symbols Symbols are used in this Instruction Manual to highlight important information:

Symbol Description Information Note:  Additional information about the current topic or proce- dure. 0918.113527680019 sigma-aldrich.com Ordering Information Trademarks SURE/CUT and HIGH PURE are a trademarks of Roche. Product Application Packsize Cat. No. All third party product names and trademarks are the property of Rapid DNA Ligation of sticky- Kit (40 DNA 11 635 379 001 their respective owners. Ligation Kit end or blunt-end ligations) DNA fragments in Regulatory Disclaimer just 5 min at 15 – 25°C. For life science research only. Not for use in diagnostic procedures. T4 DNA Ligase Ligation of sticky- 100 U 10 481 220 001 and blunt ended 500 units 10 716 359 001 Disclaimer of License DNA fragments. (1 U/␮l) For patent license limitations for individual products please refer to: rAPid Alkaline 1,000 U 04 898 133 001 List of biochemical reagent products phosphatase Alkaline Phos- Dephosphorylation 1,000 U 11 097 075 001 phatase (AP), of 5´-phosphate (20 U/␮l) special quality residues from for molecular nucleic acids. biology Agarose MP Multipurpose 100 g 11 388 983 001 agarose for analyti- 500 g 11 388 991 001 cal and preparative electrophoresis of nucleic acids Agarose LM- Low melting point 50 g 11 441 345 001 MP agarose allows 100 g 11 441 353 001 enzymatic manipu- lations Agarose Gel For the elution of 1 Kit (max. 100 11 696 505 001 DNA Extraction DNA fragments reactions) Kit from agarose gels. High Pure PCR Purification of PCR 50 purifications 11 732 668 001 Product Purifi- or enzymatic modifi-250 purifications 11 732 676 001 cation Kit cation reaction (e.g. restriction digest) SuRE/Cut Buf- Incubation buffers 1 ml each (10× 11 082 035 001 fer Set for A,B,L,M and H for conc. solutions) Restriction restriction enzymes Enzymes SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 959 001 fer A incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 967 001 fer B incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 991 001 fer H incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 975 001 fer L incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 983 001 fer M incubation conc. solution) Water, PCR Specially purified, 100 ml 03 315 843 001 Grade double-distilled, (4 vials of 25 ml) deionized, and auto-25 ml 03 315 932 001 claved (25 vials of 1 ml) 25 ml 03 315 959 001 (1 vial of 25 ml) BSA, special Maintaining 20 mg (1 ml) 10 711 454 001 quality for enzyme stability molecular biology

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