bioRxiv preprint doi: https://doi.org/10.1101/328856; this version posted May 25, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Hydrogen-based metabolism – an ancestral trait in lineages sibling to the Cyanobacteria 2 3 Paula B. Matheus Carnevali^1, Frederik Schulz^2, Cindy J. Castelle1, Rose Kantor1,13, Patrick 4 Shih3,4,14, Itai Sharon1,15,16, Joanne M. Santini5, Matthew Olm6, Yuki Amano7,8, Brian C. 5 Thomas1, Karthik Anantharaman1,17, David Burstein1,18, Eric D. Becraft9, Ramunas 6 Stepanauskas9, Tanja Woyke2 and Jillian F. Banfield*1,6,10,11,12 7 8 9 ^Contributed equally 10 *Corresponding Author (
[email protected]) 11 12 13 14 1Department of Earth and Planetary Science, University of California, Berkeley, CA, USA. 15 2DOE Joint Genome Institute, Walnut Creek, CA, USA. 16 3Feedstocks Division, Joint BioEnergy Institute, Emeryville, CA, USA. 17 4Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 18 USA. 19 5Institute of Structural & Molecular Biology, Division of Biosciences, University College London, London, UK. 20 6Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA. 21 7Nuclear Fuel Cycle Engineering Laboratories, Japan Atomic Energy Agency, Tokai, Ibaraki, Japan. 22 8Horonobe Underground Research Center, Japan Atomic Energy Agency, Horonobe, Hokkaido, Japan. 23 9Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, USA. 24 10Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA. 25 11Chan Zuckerberg Biohub, San Francisco, CA, USA. 26 12Innovative Genomics Institute, Berkley, CA, USA.