Ecologica Montenegrina 35: 115-122 (2020) This journal is available online at: www.biotaxa.org/em http://dx.doi.org/10.37828/em.2020.35.8

https://zoobank.org/urn:lsid:zoobank.org:pub:494B0359-595E-4285-B0F0-ED1C1B09CD7D

Anarta insolita umay, a new subspecies from Russian Altai and Mongolia, with re-characterization of insolita uigurica (Hacker, 1998) (, , Noctuinae)

ANTON V. VOLYNKIN1, 2, 5, SERGEY V. TITOV3 & MATJAŽ ČERNILA4

1 Altai State University, Lenina Avenue, 61, RF-656049, Barnaul, Russia. E-mail: [email protected] 2 National Research Tomsk State University, Lenina Avenue, 36, RF-634050, Tomsk, Russia 3 Department of Biology and Ecology; the Research Centre for Environmental "Monitoring", Pavlodar State University, Lomova str. 64, KZ-140008, Pavlodar, Kazakhstan 4 The Slovenian Museum of Natural History, Prešernova 20, SI-1001, Ljubljana, Slovenia. E-mail: [email protected] 5 Corresponding author

Received 29 September 2020 │ Accepted by V. Pešić: 18 October 2020 │ Published online 21 October 2020.

Abstract It is stated that the populations of Anarta insolita (Staudinger, 1889) from Eastern Kazakhstan and Mongolia formerly considered as Anarta insolita uigurica (Hacker, 1998) represent two subspecies well-separated morphologically. A new subspecies A. insolita umay Volynkin, Titov & Černila, ssp. n. is described for the populations from the southeastern Russian Altai Mts and Mongolia. The true male of A. insolita uigurica from Eastern Kazakhstan is illustrated for the first time, a diagnosis for the subspecies is provided.

Key words: Central Asia, Kazakhstan, Russian Altai, Mongolia, new subspecies, male genitalia.

Introduction

Anarta Ochsenheimer is a large Holarctic Hadeninae comprising more than 80 valid species subdivided into eight subgenera: Anarta, Tricholea Grote (= Staudinger), Ptochicestra Hacker, Aglossestra Hampson, Cardiestra Boursin, Calocestra Beck, Pulchrohadula Hacker and Hampson (Hacker 1998; Fibiger & Hacker 2005). The Eurasiatic taxa of the genus were revised by Hacker (1998). Anarta (Tricholea) insolita (Staudinger, 1889) is distributed in Central Asia. In his revision, Hacker described the subspecies A. insolita uigurica (Hacker, 1998) for the populations from Mongolia and Eastern Kazakhstan. The holotype female of uigurica originates from Eastern Kazakhstan, while all other paratypes (except for a female from SE Kazakhstan) are from Mongolia and have some slight external differences from the holotype. Later, this taxon was reported for the southeastern part of the Russian Altai Mts by Volynkin et al. (2011). During the entomological expedition to Eastern Kazakhstan in early May of 2015, the senior and second authors of the present paper collected a series of both sexes of A. insolita uigurica one hundred

Ecologica Montenegrina, 35, 2020, 115-122

NEW SUBSPECIES OF ANARTA INSOLITA FROM RUSSIAN ALTAI kilometers from the type locality. All the specimens are similar externally to the holotype and different from the populations from Mongolia and Russian Altai. Dissection of males displayed significant genital differences from both the nominate subspecies and the populations from Mongolia and Russian Altai, that proved belonging of the eastern populations to another taxon. However, COI sequences of two specimens of uigurica have no diagnostic differences from those of two specimens from Western Mongolia therefore we can consider uigurica as a subspecies of A. insolita only. At the same time, with the separation of A. insolita uigurica as an Eastern Kazakhstan subspecies the description of a new subspecies for the populations from Mongolia and Russian Altai became necessary. The subspecies is described below.

Material and methods

The genitalia of specimens were dissected and mounted in Euparal on glass slides. Photos of imagoes were taken using the camera Nikon D3100/AF-S Nikkor, 18–55 mm. Photos of the genitalia were taken by the same camera attached to a microscope with an LM-scope adapter. DNA barcode sequences of the mitochondrial cytochrome oxidase c subunit (COI barcodes) were obtained through sequencing at the Canadian Centre for DNA Barcoding (CCDB, Guelph). The barcode sequences were compared using neighbour-joining trees constructed using the Kimura-2-Parameter distance model. Abbreviations for collections used are as follows: CAV = collection of Anton Volynkin (Leominster, UK); GRB = collection of Gábor Ronkay (Budapest, Hungary); HNHM = Hungarian Natural History Museum (Budapest, Hungary); MCK = collection of Matjaž Černila (Kamnik, Slovenia); PGM = collection of Péter Gyulai (Miskolc, Hungary); ZMB = Museum of Natural History, Berlin (Museum für Naturkunde, Berlin, Germany); ZSM = the Bavarian State Collection of Zoology (Zoologische Staatssammlung München, Munich, Germany); ZVD = collection of Zoltán Varga (Debrecen, Hungary). Other abbreviations used: HT = holotype; LT = lectotype; PT = paratype.

Anarta (Tricholea) insolita umay Volynkin, Titov & Černila, ssp. n. (Figs 1–4, 9, 10, 13, 15)

Type material. Holotype (Figs 1, 9): male, “04.VI.2010, Russia, Altai Republic, Kosh-Agach District, foot of Kuraisky Ridge near Chuya Steppe, 5 km E of Chagan-Uzun village, stony steppe, 50°5'41.82"N 88°25'33.32"E, 2130 m. Volynkin A.V. leg.”, genital slide AV0842 Volynkin (Coll. ZSM, ex coll. CAV).

Paratypes: RUSSIA: 2 males, same data as for the holotype (Coll. CAV); 4 males, Russian fed., Altai mtns., Kosh-Agach distr., Chagan-Uzun, 10.station, 2280 m a.s.l., 050°05'45"N, 088°25'35"E, Černila M. (Coll. MCK); 1 male, Russian fed., Altai mtns., Kosh-Agach distr., Ulandryk r., 2050m a.s.l., 049°40'43"N, 089°04'46"E, 21–22.VI.2011, Černila M., Nakonechny A.N. (Coll. MCK); 1 male, 12.VII.2009, Altai Republic, Kosh-Agach district, Chuya steppe, 6 km SE of Chagan-Uzun village, steppe. 1800m. N 50º04', E 88º24' By light. Volynkin A.V., Černila M. & Nakonechnyi A.N. leg. (Coll. CAV); MONGOLIA: 3 females, Bayan-Ölgii aimak, NE coast of Tolbo Nuur Lake, 2100m, 1.VII.1968, exp. Kaszab (Coll. HNHM); 1 female, Bayanchongor aimak, Mts., Ih Bogd Uul, 1850 m, valley of Pitut river, 45°00’ N, 100°13’ E, 24- 26.VII.1987, leg. L. Peregovits, M. Hreblay, & T. Stéger (Coll. PGM); 1 female, Dundgovi aimak, 22 km S of Mandalgovi, 8.V.1990, leg. Fábián, Hreblay, Peregovits & Ronkay (Coll. GRB); 1 male, Dundgovi aimak, Mandalgovi, leg. Varga, genital slide 5936 Varga (Coll. ZVD); 1 male, Ömnögovi aimak, Naran Bulag, 1500m, 14.V.1990, leg. Fábián, Hreblay, Peregovits & Ronkay, genital slide 9937 Hacker (Coll. HNHM); 1 female, Ömnögovi aimak, Gurvantos, 1300m, 12–14.V.1990, leg. Fábián, Hreblay, Peregovits & Ronkay, genital slide 9938 Hacker (Coll. HNHM) (former paratypes of A. i. uigurica); 1 male, 1 female, 30– 31.05.2011, SW Mongolia, Govi-Altai aimak, Mongolian Altai Mts. (S. slope), Mogoijn-Gol valley, h=1800 m, 45°39’ N, 93°47’ E. Yakovlev R.V. leg., genital slide AV1480 (female) Volynkin (Coll. CAV); 2 males, 06–08.07.2010, SW Mongolia, Govi-Altai aimak, Mongolian Altai Mts. (S slope), Mogoijn-Gol Valley, 1800m, 45º39' N, 93º47' E, Yakovlev R.V. & Guskova E.V. leg., genital slide AV0893 Volynkin (Coll. CAV); 1 female, 29.05.2011, W Mongolia, Khovd aimak, near of Altan-Soembo, Uvchugijn-Serven Mt., 1700m, 45°39’N, 93°21’E. Yakovlev R.V. leg. (Coll. CAV); 1 female, 19.06.2005, W Mongolia, Khovd aimak, Bodonchijn-Gol river basin, Tsagduultai river valley, 2000 m, Yakovlev R.V. leg. (Coll. CAV).

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Figures 1–8. Anarta insolita sspp.: adults. Depositories of specimens: 1 in ZSM; 2–6 in CAV; 7 in PGM (photo by P. Gyulai); 8 in ZMB.

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Diagnosis. The new subspecies differs externally from A. i. uigurica in its less contrast forewing pattern, paler claviform stigma, slightly narrower and more monotonous reniform stigma, narrower and more interrupted inner blackish brown shade of the subterminal line, and narrower and more diffuse discal spot of hindwing. In comparison to the nominate subspecies (Fig. 8), A. i. umay ssp. n. has the slightly smaller size and narrower forewing with a less elongate apex, the ochreous brown forewing coloration (sand yellowish in A. i. insolita), the conspicuously more contrast forewing pattern with a well-developed subterminal line, the smaller orbicular stigma with a clearly distinguishable fringe (that is indistinct in A. i. insolita), the narrower reniform stigma with a clearly distinguishable fringe and a dark grey suffusion in its posterior half (in A. i. insolita that is monotonous with an indistinct fringe), and the broader discal spot of hindwing. The male genitalia of A. i. umay ssp. n. are very similar to those of A. i. uigurica (illustrated by Hacker (1998)), but in A. i. umay ssp. n. the right saccular process is slightly less elongate, whereas in A. i. insolita that is more elongate and reaches the tip of cucullus. The male genitalia of A. i. umay ssp. n. differ clearly from those of A. i. uigurica in the narrower uncus, the more elongate and more heavily sclerotized juxta, the narrower right saccular process with a pointed tip (whereas in A. i. uigurica the right saccular process is more robust and apically broadened and densely dentate), and the slightly larger cornutus in aedeagus vesica. The female genitalia of the three subspecies are very similar, but those of A. i. umay ssp. n. have the slightly shorter apophyses anteriores and slightly more heavily sclerotized longitudinal ribs of the posterior section of corpus bursae than those structures of A. i. uigurica. Additionally, the anterior section of corpus bursae of the new subspecies bears a narrowly lanceolate, weakly sclerotized signum, which is absent in A. i. uigurica. The distal female abdominal segments of A. i. umay ssp. n. (Fig. 15) are narrower than those of A. i. uigurica (Fig. 16). Compared to the nominate subspecies (illustrated by Hacker (1998)), the female genitalia of A. i. umay ssp. n. have the slightly less elongate and more weakly sclerotized longitudinal ribs of the posterior section of corpus bursae.

Description. External morphology of adults (Figs 1–4). Forewing length 14–17 mm in males and 16–17 mm in females. Male antenna ciliate, female antenna filiform. Body ochreous brown with slight admixture of dark brown scales. Forewing moderately broad, triangular. Forewing ground color ochreous brown with slight admixture of dark brown scales. Basal line double, brown but blackish on costa, wavy, interrupted on veins. Antemedial line double, brown but blackish on costa, irregularly wavy. Claviform stigma short, with rounded tip, pale brown, sometimes indistinct. Orbicular stigma round, pale ochreous, fringed with brown scales. Reniform stigma moderately large, with concave outer margin, fringed with brown scales, its anterior half pale ochreous, while posterior one with strong grey suffusion. Postmedial line double, brown but blackish on costa, its inner line irregularly wavy, while outer one irregularly dentate. Subterminal line dark brown, irregularly dentate, its posterior half indistinct between veins, while anterior one fringed with blackish brown shades inwardly. Terminal line thin, blackish brown, interrupted between veins. Outer wing margin slightly wavy, cilia pale ochreous. Hindwing pale ochreous with brown suffusion basally and medially, and brown in outer third. Discal spot large, semilunar. Cilia pale ochreous. Male genitalia (Figs 9, 10). Uncus broad, dorso-ventrally flattened, elliptical, with narrow base, densely setose ventrally. Tegumen short, penicular lobe broad, rounded. Juxta elongate and narrow, curved medially, heavily sclerotized. Vinculum short but robust, V-shaped with rounded tip. Valva elongate, moderately broad, with narrow cucullus with rounded tip; corona presented. Costa narrow but heavily sclerotized, with short, broad, rounded and swollen extension directed distally-ventrally. Sacculi large, strongly asymmetrical: left one very short with broad and rounded tip, while right one with long, distally narrowed and apically pointed and weakly dentate, completely covers clasper. Clasper nearly straight with slightly convex outer margin, parallel the ventral margin of valva, distally foot-like broadened. Aedeagus elongate, with large coecum, slightly curved medially and narrowed distally. Vesica with short semiglobular subbasal dorsal diverticulum and elongate, medially curved and distally strongly narrowed distal diverticulum bearing small spine-like apical cornutus; vesica ejaculatorius moderately broad, originates subbasally, directed ventrally. Female genitalia (Fig. 13). Ovipositor short, broad, conical; papilla analis trapezoid with rounded corners, densely setose. Apophyses thin, apophyses posteriores ca. twice longer than apophyses anteriores. Ostium bursae moderately broad, with medial concavity ventrally. Ductus bursae short, heavily sclerotised, dorso-ventrally flattened. Posterior section of corpus bursae narrow and elongate, with numerous longitudinal sclerotized ribs. Anterior section of corpus bursae pear-shaped, membranous, with narrowly lanceolate weakly sclerotized signum. Appendix bursae very short and moderately broad, rugose, situated latero-ventrally at junction to ductus bursae.

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Figures 9–12. Anarta insolita sspp.: male genitalia. Depositories of specimens: 9 in ZSM; 10–12 in CAV.

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Figures 13–16. Anarta insolita sspp.: female genitalia (13, 14) and female distal abdominal segments (15, 16). Specimens are deposited in CAV.

Molecular data. COI 5‘ sequences of two specimens of A. i. umay ssp. n. from Western Mongolia (Mogoijn-Gol valley, BOLD vouchers BC ZSM Lep 90261 and 90262) were compared with COI 5‘ sequences of two specimens of A. i. uigurica (BOLD vouchers BC ZSM Lep 90263 and 90264). The COI 5‘ sequences of the two subspecies have no diagnostic differences. The 658 b. p. length COI 5‘ sequence of the A. i. umay ssp. n. paratype (BOLD voucher BC ZSM Lep 90263) is as follows: AACATTATATTTTATTTTTGGAATTTGAGCAGGAATAGTAGGAACTTCATTAAGATTATTAATTC GAGCTGAATTAGGAAATCCTGGATCTTTAATTGGAGATGATCAAATTTATAATACTATTGTTAC AGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATTG ACTTGTCCCATTAATATTAGGAGCTCCTGATATAGCATTTCCTCGAATAAACAATATAAGTTTTT GACTCTTACCCCCATCTCTAACTCTTTTAATTTCAAGTAGAATTGTAGAAAATGGAGCAGGAAC AGGATGAACAGTGTACCCCCCACTTTCATCTAATATTGCACATGGAGGAAGATCAGTAGATTTA GCTATTTTTTCCCTTCATTTAGCTGGTATTTCATCTATTCTTGGAGCTATTAATTTTATTACTACA ATTATCAATATACGATTAAATAGTTTATCCTTTGATCAAATACCTTTATTTATTTGAGCTGTAGG AATTACCGCATTTTTATTATTATTATCACTTCCTGTATTAGCTGGAGCTATTACTATACTTTTAAC TGATCGAAATTTAAATACATCTTTTTTTGATCCTGCTGGTGGAGGTGACCCAATTTTATATCAAC ATTTATTT.

Distribution. The new subspecies is known from the southeastern part of the Russian Altai Mts (Kosh- Agach District: Chuya Steppe), and western and southern Mongolia (Bayan-Ölgii, Khovd, Govi-Altai, Dundgovi and Ömnögovi Aimags).

Etymology. In Turkic mythology, Umay is the goddess of fertility and the patroness of children.

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Anarta (Tricholea) insolita uigurica (Hacker, 1998) (Figs 5–7, 11, 12, 14, 16)

Hadula insolita uigurica Hacker, 1998, Esperiana 6: 599, Plate Hadula I, fig. 5 (Type locality: [East Kazakhstan Region, Kurchum District, near Kaiyndy Village, Narym Ridge] “Kasachstan, Süd-Altaij, Slavianka, Narym-Gebirge, 420m, 48°51’N 83°32’E”).

Type material examined: photographs of the holotype (Fig. 7), female, dark green label “Kasachstan, Süd- Altaij, Slavianka, Narym-Gebirge, 420m, 48°51’N 83°32’E, 1993.V.26. Leg.: V. & A. Lukhtanov | Dr. P. Gyulai, Hungary” / red label “HT” / red label “Hadula-Revision, ESPERIANA VI (1998), Hadula insolita ssp. uigurica Hacker, Holotypus” / yellow label “Gen. Präp [genital slide] Hacker N 10509” (Coll. PGM, later to be deposited in HNHM).

Additional material examined. 7 males, 4 females, 06.V.2015, E Kazakhstan, Kurchum District, 15.5 km NNE of Amanat village, Kiin-Kirish Massif, clay/chalk hills, 447m, N48°7.885’ E84°29.378’, Volynkin A.V. & Titov S.V. leg., genital slides AV1465, AV1478 (males), AV1477, AV1479 (females) Volynkin (Coll. CAV).

Diagnosis. Forewing length is 14–16 mm in males and 14–17 mm in females. Anarta insolita uigurica can be distinguished from the two other subspecies by its more contrast pattern and more distinct reniform and orbicular stigmata. The male genitalia of A. insolita uigurica differ clearly from the two other subspecies by the broader uncus, the slightly broader cucullus, and the right saccular process being robust and apically broadened and densely dentate (whereas in A. i. insolita and A. i. umay ssp. n. that is distally narrowed, apically pointed and weakly dentate apically). The female genitalia of A. i. uigurica differ from those of A. i. insolita and A. i. umay ssp. n. in the shortest and most weakly sclerotized longitudinal ribs of the posterior section of corpus bursae and the absence of signum bursae (present in the two other subspecies). The detailed comparison with A. i. umay ssp. n. is provided above in the ‘Diagnosis’ chapter of the latter.

Molecular data. The COI 5‘ sequences of two specimens of A. i. uigurica (BOLD vouchers BC ZSM Lep 90263 and 90264) display variability in loci 444 (C/T), 612 (C/T) and 636 (A/G). The 658 b. p. length COI 5‘ sequence of the A. i. uigurica (BOLD voucher BC ZSM Lep 90263) is as follows: AACATTATATTTTATTTTTGGAATTTGAGCAGGAATAGTAGGAACTTCATTAAGATTATTAATTC GAGCTGAATTAGGAAATCCTGGATCTTTAATTGGAGATGATCAAATTTATAATACTATTGTTAC AGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATTG ACTTGTCCCATTAATATTAGGAGCTCCTGATATAGCATTTCCTCGAATAAACAATATAAGTTTTT GACTCTTACCCCCATCTCTAACTCTTTTAATTTCAAGTAGAATTGTAGAAAATGGAGCAGGAAC AGGATGAACAGTGTACCCCCCACTTTCATCTAATATTGCACATGGAGGAAGATCAGTAGATTTA GCTATTTTTTCCCTTCATTTAGCTGGTATTTCATCTATTCTTGGAGCTATTAATTTTATTACTACA ATTATCAATATACGATTAAATAGTTTATCCTTTGATCAAATACCTTTATTTATTTGAGCTGTAGG AATTACCGCATTTTTATTATTATTATCACTTCCTGTATTAGCTGGAGCTATTACTATACTTTTAAC TGATCGAAATTTAAATACATCTTTTTTTGATCCTGCTGGTGGAGGTGACCCAATTTTATATCAAC ATTTATTT

Distribution. The subspecies is known up to date from two localities in the Kurchum District of the East Kazakhstan Region (western Kazakh Altai Mts and its foothills). The status of the population from SE Kazakhstan (Altyn Emel National Park, the record was based on a single female) (Hacker 1998) needs clarification.

Acknowledgements We express our sincere gratitude to Dr. Péter Gyulai (Miskolc, Hungary) for photos of the holotype of Anarta insolita uigurica provided and critical comments to the manuscript; Dr. Wolfram Mey (ZMB, Berlin) for his kind assistance provided during the senior author’s work at ZMB collection; and Mr. Balázs Benedek (Törökbálint, Hungary) for his critical comments to the manuscript. We are also indebted to Dr. Axel Hausmann (ZSM, Munich, Germany) for help in arranging for DNA barcoding.

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References

Fibiger, M. & Hacker, H.H. (2005) Systematic List of the of Europe (Notodontidae, Nolidae, Arctiidae, Lymantriidae, Erebidae, Micronoctuidae, and Noctuidae). Esperiana, 11, 83–172. Available from: http://esperiana.net/mediapool/86/862516/data/Esperiana_Band_11_93-206.pdf (accessed 08 August 2020) Hacker, H. (1998) Revision der Gattungen Hadula Staudinger, 1889 (= Discestra Hampson, 1905; Aglossestra, Hampson, 1905; = Cardiestra Boursin, 1963), Anartomorpha Alpheraky, 1892, Trichanarta Hampson, 1895, Anarta Ochsenheimer, 1816 und Cardepia Hampson, 1905 mit Beschreibung einer neuen Gattung Hadumorpha gen. n. (Lepidoptera, Noctuidae). Esperiana, 6, 577– 843. [in German] Volynkin A.V., Matov A.Yu., Nakonechnyi A.N., Černila M. (2011) Species of Noctuid (Lepidoptera, Noctuidae) newly recorded for the fauna of Russia from territory of Russian Altai. Euroasian Entomological Journal, 10 (1), 23–30 + 22 + plate II. [in Russian with English summary]

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