Ecologica Montenegrina 35: 115-122 (2020) This journal is available online at: www.biotaxa.org/em http://dx.doi.org/10.37828/em.2020.35.8 https://zoobank.org/urn:lsid:zoobank.org:pub:494B0359-595E-4285-B0F0-ED1C1B09CD7D Anarta insolita umay, a new subspecies from Russian Altai and Mongolia, with re-characterization of Anarta insolita uigurica (Hacker, 1998) (Lepidoptera, Noctuidae, Noctuinae) ANTON V. VOLYNKIN1, 2, 5, SERGEY V. TITOV3 & MATJAŽ ČERNILA4 1 Altai State University, Lenina Avenue, 61, RF-656049, Barnaul, Russia. E-mail: [email protected] 2 National Research Tomsk State University, Lenina Avenue, 36, RF-634050, Tomsk, Russia 3 Department of Biology and Ecology; the Research Centre for Environmental "Monitoring", Pavlodar State University, Lomova str. 64, KZ-140008, Pavlodar, Kazakhstan 4 The Slovenian Museum of Natural History, Prešernova 20, SI-1001, Ljubljana, Slovenia. E-mail: [email protected] 5 Corresponding author Received 29 September 2020 │ Accepted by V. Pešić: 18 October 2020 │ Published online 21 October 2020. Abstract It is stated that the populations of Anarta insolita (Staudinger, 1889) from Eastern Kazakhstan and Mongolia formerly considered as Anarta insolita uigurica (Hacker, 1998) represent two subspecies well-separated morphologically. A new subspecies A. insolita umay Volynkin, Titov & Černila, ssp. n. is described for the populations from the southeastern Russian Altai Mts and Mongolia. The true male of A. insolita uigurica from Eastern Kazakhstan is illustrated for the first time, a diagnosis for the subspecies is provided. Key words: Central Asia, Kazakhstan, Russian Altai, Mongolia, new subspecies, male genitalia. Introduction Anarta Ochsenheimer is a large Holarctic Hadeninae genus comprising more than 80 valid species subdivided into eight subgenera: Anarta, Tricholea Grote (= Hadula Staudinger), Ptochicestra Hacker, Aglossestra Hampson, Cardiestra Boursin, Calocestra Beck, Pulchrohadula Hacker and Discestra Hampson (Hacker 1998; Fibiger & Hacker 2005). The Eurasiatic taxa of the genus were revised by Hacker (1998). Anarta (Tricholea) insolita (Staudinger, 1889) is distributed in Central Asia. In his revision, Hacker described the subspecies A. insolita uigurica (Hacker, 1998) for the populations from Mongolia and Eastern Kazakhstan. The holotype female of uigurica originates from Eastern Kazakhstan, while all other paratypes (except for a female from SE Kazakhstan) are from Mongolia and have some slight external differences from the holotype. Later, this taxon was reported for the southeastern part of the Russian Altai Mts by Volynkin et al. (2011). During the entomological expedition to Eastern Kazakhstan in early May of 2015, the senior and second authors of the present paper collected a series of both sexes of A. insolita uigurica one hundred Ecologica Montenegrina, 35, 2020, 115-122 NEW SUBSPECIES OF ANARTA INSOLITA FROM RUSSIAN ALTAI kilometers from the type locality. All the specimens are similar externally to the holotype and different from the populations from Mongolia and Russian Altai. Dissection of males displayed significant genital differences from both the nominate subspecies and the populations from Mongolia and Russian Altai, that proved belonging of the eastern populations to another taxon. However, COI sequences of two specimens of uigurica have no diagnostic differences from those of two specimens from Western Mongolia therefore we can consider uigurica as a subspecies of A. insolita only. At the same time, with the separation of A. insolita uigurica as an Eastern Kazakhstan subspecies the description of a new subspecies for the populations from Mongolia and Russian Altai became necessary. The subspecies is described below. Material and methods The genitalia of specimens were dissected and mounted in Euparal on glass slides. Photos of imagoes were taken using the camera Nikon D3100/AF-S Nikkor, 18–55 mm. Photos of the genitalia were taken by the same camera attached to a microscope with an LM-scope adapter. DNA barcode sequences of the mitochondrial cytochrome oxidase c subunit (COI barcodes) were obtained through sequencing at the Canadian Centre for DNA Barcoding (CCDB, Guelph). The barcode sequences were compared using neighbour-joining trees constructed using the Kimura-2-Parameter distance model. Abbreviations for collections used are as follows: CAV = collection of Anton Volynkin (Leominster, UK); GRB = collection of Gábor Ronkay (Budapest, Hungary); HNHM = Hungarian Natural History Museum (Budapest, Hungary); MCK = collection of Matjaž Černila (Kamnik, Slovenia); PGM = collection of Péter Gyulai (Miskolc, Hungary); ZMB = Museum of Natural History, Berlin (Museum für Naturkunde, Berlin, Germany); ZSM = the Bavarian State Collection of Zoology (Zoologische Staatssammlung München, Munich, Germany); ZVD = collection of Zoltán Varga (Debrecen, Hungary). Other abbreviations used: HT = holotype; LT = lectotype; PT = paratype. Anarta (Tricholea) insolita umay Volynkin, Titov & Černila, ssp. n. (Figs 1–4, 9, 10, 13, 15) Type material. Holotype (Figs 1, 9): male, “04.VI.2010, Russia, Altai Republic, Kosh-Agach District, foot of Kuraisky Ridge near Chuya Steppe, 5 km E of Chagan-Uzun village, stony steppe, 50°5'41.82"N 88°25'33.32"E, 2130 m. Volynkin A.V. leg.”, genital slide AV0842 Volynkin (Coll. ZSM, ex coll. CAV). Paratypes: RUSSIA: 2 males, same data as for the holotype (Coll. CAV); 4 males, Russian fed., Altai mtns., Kosh-Agach distr., Chagan-Uzun, 10.station, 2280 m a.s.l., 050°05'45"N, 088°25'35"E, Černila M. (Coll. MCK); 1 male, Russian fed., Altai mtns., Kosh-Agach distr., Ulandryk r., 2050m a.s.l., 049°40'43"N, 089°04'46"E, 21–22.VI.2011, Černila M., Nakonechny A.N. (Coll. MCK); 1 male, 12.VII.2009, Altai Republic, Kosh-Agach district, Chuya steppe, 6 km SE of Chagan-Uzun village, steppe. 1800m. N 50º04', E 88º24' By light. Volynkin A.V., Černila M. & Nakonechnyi A.N. leg. (Coll. CAV); MONGOLIA: 3 females, Bayan-Ölgii aimak, NE coast of Tolbo Nuur Lake, 2100m, 1.VII.1968, exp. Kaszab (Coll. HNHM); 1 female, Bayanchongor aimak, Mts., Ih Bogd Uul, 1850 m, valley of Pitut river, 45°00’ N, 100°13’ E, 24- 26.VII.1987, leg. L. Peregovits, M. Hreblay, & T. Stéger (Coll. PGM); 1 female, Dundgovi aimak, 22 km S of Mandalgovi, 8.V.1990, leg. Fábián, Hreblay, Peregovits & Ronkay (Coll. GRB); 1 male, Dundgovi aimak, Mandalgovi, leg. Varga, genital slide 5936 Varga (Coll. ZVD); 1 male, Ömnögovi aimak, Naran Bulag, 1500m, 14.V.1990, leg. Fábián, Hreblay, Peregovits & Ronkay, genital slide 9937 Hacker (Coll. HNHM); 1 female, Ömnögovi aimak, Gurvantos, 1300m, 12–14.V.1990, leg. Fábián, Hreblay, Peregovits & Ronkay, genital slide 9938 Hacker (Coll. HNHM) (former paratypes of A. i. uigurica); 1 male, 1 female, 30– 31.05.2011, SW Mongolia, Govi-Altai aimak, Mongolian Altai Mts. (S. slope), Mogoijn-Gol valley, h=1800 m, 45°39’ N, 93°47’ E. Yakovlev R.V. leg., genital slide AV1480 (female) Volynkin (Coll. CAV); 2 males, 06–08.07.2010, SW Mongolia, Govi-Altai aimak, Mongolian Altai Mts. (S slope), Mogoijn-Gol Valley, 1800m, 45º39' N, 93º47' E, Yakovlev R.V. & Guskova E.V. leg., genital slide AV0893 Volynkin (Coll. CAV); 1 female, 29.05.2011, W Mongolia, Khovd aimak, near of Altan-Soembo, Uvchugijn-Serven Mt., 1700m, 45°39’N, 93°21’E. Yakovlev R.V. leg. (Coll. CAV); 1 female, 19.06.2005, W Mongolia, Khovd aimak, Bodonchijn-Gol river basin, Tsagduultai river valley, 2000 m, Yakovlev R.V. leg. (Coll. CAV). 116 VOLYNKIN ET AL. Figures 1–8. Anarta insolita sspp.: adults. Depositories of specimens: 1 in ZSM; 2–6 in CAV; 7 in PGM (photo by P. Gyulai); 8 in ZMB. Ecologica Montenegrina, 35, 2020, 115-122 117 NEW SUBSPECIES OF ANARTA INSOLITA FROM RUSSIAN ALTAI Diagnosis. The new subspecies differs externally from A. i. uigurica in its less contrast forewing pattern, paler claviform stigma, slightly narrower and more monotonous reniform stigma, narrower and more interrupted inner blackish brown shade of the subterminal line, and narrower and more diffuse discal spot of hindwing. In comparison to the nominate subspecies (Fig. 8), A. i. umay ssp. n. has the slightly smaller size and narrower forewing with a less elongate apex, the ochreous brown forewing coloration (sand yellowish in A. i. insolita), the conspicuously more contrast forewing pattern with a well-developed subterminal line, the smaller orbicular stigma with a clearly distinguishable fringe (that is indistinct in A. i. insolita), the narrower reniform stigma with a clearly distinguishable fringe and a dark grey suffusion in its posterior half (in A. i. insolita that is monotonous with an indistinct fringe), and the broader discal spot of hindwing. The male genitalia of A. i. umay ssp. n. are very similar to those of A. i. uigurica (illustrated by Hacker (1998)), but in A. i. umay ssp. n. the right saccular process is slightly less elongate, whereas in A. i. insolita that is more elongate and reaches the tip of cucullus. The male genitalia of A. i. umay ssp. n. differ clearly from those of A. i. uigurica in the narrower uncus, the more elongate and more heavily sclerotized juxta, the narrower right saccular process with a pointed tip (whereas in A. i. uigurica the right saccular process is more robust and apically broadened and densely dentate), and the slightly larger cornutus in aedeagus vesica. The female genitalia of the three subspecies are very similar, but those of A. i. umay ssp. n. have the slightly shorter apophyses anteriores and slightly more heavily sclerotized longitudinal ribs of the posterior section of corpus bursae than those structures of A. i. uigurica. Additionally, the anterior section of corpus bursae of the new subspecies bears a narrowly lanceolate, weakly sclerotized signum, which is absent in A. i. uigurica. The distal female abdominal segments of A. i. umay ssp. n. (Fig. 15) are narrower than those of A. i. uigurica (Fig. 16). Compared to the nominate subspecies (illustrated by Hacker (1998)), the female genitalia of A. i. umay ssp. n.