Vibrio Parahaemolyticus Host Pathogen
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Characterization and Complete Genome Sequence of Bacteriophage Vb Vc Srvc2, a Marine Phage That Infects Vibrio Campbellii
Characterization and complete genome sequence of bacteriophage vB_Vc_SrVc2, a marine phage that infects Vibrio campbellii Carlos Omar Lomelí-Ortega Centro de Investigaciónes Biológicas del Noroeste: Centro de Investigaciones Biologicas del Noroeste SC Alexis de Jesús Martínez-Sández Universidad Autónoma de Baja California Sur: Universidad Autonoma de Baja California Sur Diana Barajas-Sandoval Centro de Investigaciónes Biológicas del Noroeste: Centro de Investigaciones Biologicas del Noroeste SC Francisco Javier Magallón-Barajas Centro de Investigaciónes Biológicas del Noroeste: Centro de Investigaciones Biologicas del Noroeste SC Andrew Millard University of Leicester Juan Manuel Martínez-Villalobos Universidad Autónoma de Nuevo León: Universidad Autonoma de Nuevo Leon Elva Teresa Arechiga-Carvajal Universidad Autonoma de Nuevo Leon Eduardo Quiroz-Guzman ( [email protected] ) Centro de Investigaciones Biologicas del Noroeste SC https://orcid.org/0000-0002-4776-4564 Research Article Keywords: Vibrio campbellii, pathogen, bacteriophage, phage therapy Posted Date: August 20th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-779229/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/22 Abstract Vibrio campbellii is widely distributed in the marine environment and is an important pathogen of aquatic organisms such as shrimp, sh, and mollusks. The emergence of multi-drug resistance among these bacteria resulted in a worldwide public health problem, which requires alternative treatment approaches such as phage therapy. In the present study, we isolated a phage vB_Vc_SrVc2 from white shrimp hepatopancreas with symptoms of AHPND. Phage vB_Vc_SrVc2 is a member of the genus Maculvirus and the family Autographiviridae, with high lytic ability against Vibrio isolates. -
Delineating Virulence of Vibrio Campbellii
www.nature.com/scientificreports OPEN Delineating virulence of Vibrio campbellii: a predominant luminescent bacterial pathogen in Indian shrimp hatcheries Sujeet Kumar1*, Chandra Bhushan Kumar1,2, Vidya Rajendran1, Nishawlini Abishaw1, P. S. Shyne Anand1, S. Kannapan1, Viswas K. Nagaleekar3, K. K. Vijayan1 & S. V. Alavandi1 Luminescent vibriosis is a major bacterial disease in shrimp hatcheries and causes up to 100% mortality in larval stages of penaeid shrimps. We investigated the virulence factors and genetic identity of 29 luminescent Vibrio isolates from Indian shrimp hatcheries and farms, which were earlier presumed as Vibrio harveyi. Haemolysin gene-based species-specifc multiplex PCR and phylogenetic analysis of rpoD and toxR identifed all the isolates as V. campbellii. The gene-specifc PCR revealed the presence of virulence markers involved in quorum sensing (luxM, luxS, cqsA), motility (faA, lafA), toxin (hly, chiA, serine protease, metalloprotease), and virulence regulators (toxR, luxR) in all the isolates. The deduced amino acid sequence analysis of virulence regulator ToxR suggested four variants, namely A123Q150 (AQ; 18.9%), P123Q150 (PQ; 54.1%), A123P150 (AP; 21.6%), and P123P150 (PP; 5.4% isolates) based on amino acid at 123rd (proline or alanine) and 150th (glutamine or proline) positions. A signifcantly higher level of the quorum-sensing signal, autoinducer-2 (AI-2, p = 2.2e−12), and signifcantly reduced protease activity (p = 1.6e−07) were recorded in AP variant, whereas an inverse trend was noticed in the Q150 variants AQ and PQ. The pathogenicity study in Penaeus (Litopenaeus) vannamei juveniles revealed that all the isolates of AQ were highly pathogenic with Cox proportional hazard ratio 15.1 to 32.4 compared to P150 variants; PP (5.4 to 6.3) or AP (7.3 to 14). -
Siderophores from Marine Bacteria with Special Emphasis on Vibrionaceae
Archana et al Int. J. Pure App. Biosci. 7 (3): 58-66 (2019) ISSN: 2320 – 7051 Available online at www.ijpab.com DOI: http://dx.doi.org/10.18782/2320-7051.7492 ISSN: 2320 – 7051 Int. J. Pure App. Biosci. 7 (3): 58-66 (2019) Review Article Siderophores from Marine Bacteria with Special Emphasis on Vibrionaceae Archana V.1, K. Revathi2*, V. P. Limna Mol3, R. Kirubagaran3 1Department of Advanced Zoology and Biotechnology, Madras University, Chennai- 600005 2MAHER University, Chennai, Tamil Nadu – 600078 3Ocean Science and Technology for Islands, National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences, Government of India, Pallikaranai, Chennai- 600100 *Corresponding Author E-mail: [email protected] Received: 11.04.2019 | Revised: 18.05.2019 | Accepted: 25.05.2019 ABSTRACT More than 500 siderophores have been isolated from a huge number of marine bacteria till date. With mankind’s ever-increasing search for novel molecules towards industrial and medical applications, siderophores have gained high importance. These chelating ligands have immense potential in promoting plant growth, drug-delivery, treatment of iron-overload, etc. Many of the potential siderophores have been isolated from bacteria like Pseudomonas, Bacillus, Nocardia, etc. Bacteria belonging to the family Vibrionaceae have recently gained focus owing to their rich potential in secreting siderophores. Many of the vibrionales, viz. Vibrio harveyii, V. anguillarium, V. campbellii. etc. are aquatic pathogens. These bacteria require iron for their growth and virulence, and hence produce a wide variety of siderophores. The genetic basis of siderophore production by Vibrio sp. has also been largely studied. Further detailed genetic analysis of the mode of siderophore production by Vibrionaceae would be highly effective to treat aquaculture diseases caused by these pathogenic organisms. -
Genetic Diversity of Culturable Vibrio in an Australian Blue Mussel Mytilus Galloprovincialis Hatchery
Vol. 116: 37–46, 2015 DISEASES OF AQUATIC ORGANISMS Published September 17 doi: 10.3354/dao02905 Dis Aquat Org Genetic diversity of culturable Vibrio in an Australian blue mussel Mytilus galloprovincialis hatchery Tzu Nin Kwan*, Christopher J. S. Bolch National Centre for Marine Conservation and Resource Sustainability, University of Tasmania, Locked Bag 1370, Newnham, Tasmania 7250, Australia ABSTRACT: Bacillary necrosis associated with Vibrio species is the common cause of larval and spat mortality during commercial production of Australian blue mussel Mytilus galloprovincialis. A total of 87 randomly selected Vibrio isolates from various stages of rearing in a commercial mus- sel hatchery were characterised using partial sequences of the ATP synthase alpha subunit gene (atpA). The sequenced isolates represented 40 unique atpA genotypes, overwhelmingly domi- nated (98%) by V. splendidus group genotypes, with 1 V. harveyi group genotype also detected. The V. splendidus group sequences formed 5 moderately supported clusters allied with V. splen- didus/V. lentus, V. atlanticus, V. tasmaniensis, V. cyclitrophicus and V. toranzoniae. All water sources showed considerable atpA gene diversity among Vibrio isolates, with 30 to 60% of unique isolates recovered from each source. Over half (53%) of Vibrio atpA genotypes were detected only once, and only 7 genotypes were recovered from multiple sources. Comparisons of phylogenetic diversity using UniFrac analysis showed that the culturable Vibrio community from intake, header, broodstock and larval tanks were phylogenetically similar, while spat tank communities were different. Culturable Vibrio associated with spat tank seawater differed in being dominated by V. toranzoniae-affiliated genotypes. The high diversity of V. splendidus group genotypes detected in this study reinforces the dynamic nature of microbial communities associated with hatchery culture and complicates our efforts to elucidate the role of V. -
EMBRIC (Grant Agreement No
Deliverable D6.1 EMBRIC (Grant Agreement No. 654008) Grant Agreement Number: 654008 EMBRIC European Marine Biological Research Infrastructure Cluster to promote the Blue Bioeconomy Horizon 2020 – the Framework Programme for Research and Innovation (2014-2020), H2020-INFRADEV-1-2014-1 Start Date of Project: 01.06.2015 Duration: 48 Months Deliverable D6.1 EMBRIC showcases: prototype pipelines from the microorganism to product discovery (M36) HORIZON 2020 - INFRADEV Deliverable D6.1 EMBRIC showcases: prototype pipelines from the microorganism to product discovery Page 1 of 85 Deliverable D6.1 EMBRIC (Grant Agreement No. 654008) Implementation and operation of cross-cutting services and solutions for clusters of ESFRI Grant agreement no.: 654008 Project acronym: EMBRIC Project website: www.embric.eu Project full title: European Marine Biological Research Infrastructure cluster to promote the Bioeconomy Project start date: June 2015 (48 months) Submission due date : May 2018 Actual submission date: May 2018 Work Package: WP 6 Microbial pipeline from environment to active compounds Lead Beneficiary: CABI Version: 9.0 Authors: SMITH David GOSS Rebecca OVERMANN Jörg BRÖNSTRUP Mark PASCUAL Javier BAJERSKI Felizitas HENSLER Michael WANG Yunpeng ABRAHAM Emily Deliverable D6.1 EMBRIC showcases: prototype pipelines from the microorganism to product discovery Page 2 of 85 Deliverable D6.1 EMBRIC (Grant Agreement No. 654008) Project funded by the European Union’s Horizon 2020 research and innovation programme (2015-2019) Dissemination Level PU Public PP Restricted to other programme participants (including the Commission Services) RE Restricted to a group specified by the consortium (including the Commission Services) CO Confidential, only for members of the consortium (including the Commission X Services Deliverable D6.1 EMBRIC showcases: prototype pipelines from the microorganism to product discovery Page 3 of 85 Deliverable D6.1 EMBRIC (Grant Agreement No. -
A Novel Vibriophage Vb Vcas HC Containing Lysogeny-Related Gene Has Strong Lytic Ability Against Pathogenic Bacteria
Virologica Sinica www.virosin.org https://doi.org/10.1007/s12250-020-00271-w www.springer.com/12250 (0123456789().,-volV)(0123456789().,-volV) RESEARCH ARTICLE A Novel Vibriophage vB_VcaS_HC Containing Lysogeny-Related Gene Has Strong Lytic Ability against Pathogenic Bacteria 1,2 1,2 1,2 1 3 3 Chengcheng Li • Zengmeng Wang • Jiulong Zhao • Long Wang • Guosi Xie • Jie Huang • Yongyu Zhang1,2 Received: 2 March 2020 / Accepted: 8 June 2020 Ó Wuhan Institute of Virology, CAS 2020 Abstract To avoid the negative effects of antibiotics, using phage to prevent animal disease becomes a promising method in aquaculture. Here, a lytic phage provisionally named vB_VcaS_HC that can infect the pathogen (i.e., Vibrio campbellii 18) of prawn was isolated. The phage has an isometric head and a non-contractile tail. During phage infection, the induced host mortality in 5.5 h reached ca. 96%, with a latent period of 1.5 h and a burst size of 172 PFU/cell. It has an 81,566 bp circular dsDNA genome containing 121 open reading frames (ORFs), and ca. 71% of the ORFs are functionally unknown. Comparative genomic and phylogenetic analysis revealed that it is a novel phage belonging to Delepquintavirus, Siphoviridae, Caudovirales. In the phage genome, besides the ordinary genes related to structure assembly and DNA metabolism, there are 10 auxiliary metabolic genes. For the first time, the pyruvate phosphate dikinase (PPDK) gene was found in phages whose product is a key rate-limiting enzyme involving Embden-Meyerhof-Parnas (EMP) reaction. Interestingly, although the phage has a strong bactericidal activity and contains a potential lysogeny related gene, i.e., the recombinase (RecA) gene, we did not find the phage turned into a lysogenic state. -
Induction and Genome Analysis of HY01, a Newly Reported Prophage from an Emerging Shrimp Pathogen Vibrio Campbellii
microorganisms Article Induction and Genome Analysis of HY01, a Newly Reported Prophage from an Emerging Shrimp Pathogen Vibrio campbellii Taiyeebah Nuidate 1 , Aphiwat Kuaphiriyakul 1, Komwit Surachat 2,3 and Pimonsri Mittraparp-arthorn 1,3,* 1 Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand; [email protected] (T.N.); [email protected] (A.K.) 2 Division of Computational Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand; [email protected] 3 Molecular Evolution and Computational Biology Research Unit, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand * Correspondence: [email protected]; Tel.: +66-74-288-314 Abstract: Vibrio campbellii is an emerging aquaculture pathogen that causes luminous vibriosis in farmed shrimp. Although prophages in various aquaculture pathogens have been widely reported, there is still limited knowledge regarding prophages in the genome of pathogenic V. campbellii. Here, we describe the full-genome sequence of a prophage named HY01, induced from the emerging shrimp pathogen V. campbellii HY01. The phage HY01 was induced by mitomycin C and was morphologically characterized as long tailed phage. V. campbellii phage HY01 is composed of 41,772 bp of dsDNA with a G+C content of 47.45%. A total of 60 open reading frames (ORFs) were identified, of which 31 could be predicted for their biological functions. Twenty seven out of 31 predicted protein coding regions were matched with several encoded proteins of various Enterobacteriaceae, Pseudomonadaceae, Vibrionaceae, and other phages of Gram-negative bacteria. Interestingly, the comparative genome analysis revealed that the phage HY01 was only distantly related to Vibrio phage Va_PF430-3_p42 of Citation: Nuidate, T.; Kuaphiriyakul, fish pathogen V. -
Disease of Aquatic Organisms 103:133
Vol. 103: 133–139, 2013 DISEASES OF AQUATIC ORGANISMS Published March 26 doi: 10.3354/dao02572 Dis Aquat Org Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor Qingru Jiang**, Liuyang Shi**, Caihuan Ke, Weiwei You, Jing Zhao* College of Ocean and Earth Science of Xiamen University, Xiamen 361005, China ABSTRACT: Mass mortality of farmed small abalone Haliotis diversicolor occurred in Fujian, China, from 2009 to 2011. Among isolates obtained from moribund abalones, the dominant species AP37 exhibited the strongest virulence. After immersion challenge with 106 CFU ml−1 of AP37, abalone mortalities of 0, 53 and 67% were induced at water temperatures of 20°C, 24°C, and 28°C, respectively. Following intramuscular injection, AP37 showed a low LD50 (median lethal concen- tration) value of 2.9 × 102 CFU g−1 (colony forming units per gram abalone wet body weight). The 6 −1 5 LT50 (median lethal time) values were 5.2 h for 1 × 10 CFU abalone , 8.4 h for 1 × 10 CFU abalone−1, and 21.5 h for 1 × 104 CFU abalone−1. For further analysis of virulence, AP37 was screened for the production of extracellular factors. The results showed that various factors includ- ing presence of flagella and production of extracellular enzymes, such as lipase, phospholipase and haemolysin, could be responsible for pathogenesis. Based on its 16S rRNA gene sequence, strain AP37 showed >98.8% similarity to Vibrio harveyi, V. campbellii, V. parahaemolyticus, V. algi nolyticus, V. na trie gens and V. rotiferianus, so it could not be identified by this method. -
First Evidence for a Vibrio Strain Pathogenic to Mytilus Edulis Altering Hemocyte Immune Capacities
1 Developmental & Comparative Immunology Achimer April 2016, Volume 57, Pages 107-119 http://dx.doi.org/10.1016/j.dci.2015.12.014 http://archimer.ifremer.fr http://archimer.ifremer.fr/doc/00302/41336/ © 2015 Elsevier Ltd. All rights reserved First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities Ben Cheikh Yosra 1, Travers Marie-Agnes 2, Morga Benjamin 2, Godfrin Yoann 2, Rioult Damien 3, Le Foll Frank 1, * 1 Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Le Havre, F- 76063, Le Havre Cedex, France 2 Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390, La Tremblade, France 3 Laboratory of Ecotoxicology- Aquatic Environments, UMR-I 02, SEBIO, University of Reims Champagne Ardenne, Campus Moulin de la House, F-51100, Reims, France * Corresponding author : Frank Le Foll, email address : [email protected] Abstract : Bacterial isolates were obtained from mortality events affecting Mytilus edulis and reported by professionals in 2010–2013 or from mussel microflora. Experimental infections allowed the selection of two isolates affiliated to Vibrio splendidus/Vibrio hemicentroti type strains: a virulent 10/068 1T1 (76.6% and 90% mortalities in 24 h and 96 h) and an innocuous 12/056 M24T1 (0% and 23.3% in 24 h and 96 h). These two strains were GFP-tagged and validated for their growth characteristics and virulence as genuine models for exposure. Then, host cellular immune responses to the microbial invaders were assessed. In the presence of the virulent strain, hemocyte motility was instantaneously enhanced but markedly slowed down after 2 h exposure. -
Aquatic Microbial Ecology 80:15
The following supplement accompanies the article Isolates as models to study bacterial ecophysiology and biogeochemistry Åke Hagström*, Farooq Azam, Carlo Berg, Ulla Li Zweifel *Corresponding author: [email protected] Aquatic Microbial Ecology 80: 15–27 (2017) Supplementary Materials & Methods The bacteria characterized in this study were collected from sites at three different sea areas; the Northern Baltic Sea (63°30’N, 19°48’E), Northwest Mediterranean Sea (43°41'N, 7°19'E) and Southern California Bight (32°53'N, 117°15'W). Seawater was spread onto Zobell agar plates or marine agar plates (DIFCO) and incubated at in situ temperature. Colonies were picked and plate- purified before being frozen in liquid medium with 20% glycerol. The collection represents aerobic heterotrophic bacteria from pelagic waters. Bacteria were grown in media according to their physiological needs of salinity. Isolates from the Baltic Sea were grown on Zobell media (ZoBELL, 1941) (800 ml filtered seawater from the Baltic, 200 ml Milli-Q water, 5g Bacto-peptone, 1g Bacto-yeast extract). Isolates from the Mediterranean Sea and the Southern California Bight were grown on marine agar or marine broth (DIFCO laboratories). The optimal temperature for growth was determined by growing each isolate in 4ml of appropriate media at 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50o C with gentle shaking. Growth was measured by an increase in absorbance at 550nm. Statistical analyses The influence of temperature, geographical origin and taxonomic affiliation on growth rates was assessed by a two-way analysis of variance (ANOVA) in R (http://www.r-project.org/) and the “car” package. -
D 6.1 EMBRIC Showcases
Grant Agreement Number: 654008 EMBRIC European Marine Biological Research Infrastructure Cluster to promote the Blue Bioeconomy Horizon 2020 – the Framework Programme for Research and Innovation (2014-2020), H2020-INFRADEV-1-2014-1 Start Date of Project: 01.06.2015 Duration: 48 Months Deliverable D6.1 b EMBRIC showcases: prototype pipelines from the microorganism to product discovery (Revised 2019) HORIZON 2020 - INFRADEV Implementation and operation of cross-cutting services and solutions for clusters of ESFRI 1 Grant agreement no.: 654008 Project acronym: EMBRIC Project website: www.embric.eu Project full title: European Marine Biological Research Infrastructure cluster to promote the Bioeconomy (Revised 2019) Project start date: June 2015 (48 months) Submission due date: May 2019 Actual submission date: Apr 2019 Work Package: WP 6 Microbial pipeline from environment to active compounds Lead Beneficiary: CABI [Partner 15] Version: 1.0 Authors: SMITH David [CABI Partner 15] GOSS Rebecca [USTAN 10] OVERMANN Jörg [DSMZ Partner 24] BRÖNSTRUP Mark [HZI Partner 18] PASCUAL Javier [DSMZ Partner 24] BAJERSKI Felizitas [DSMZ Partner 24] HENSLER Michael [HZI Partner 18] WANG Yunpeng [USTAN Partner 10] ABRAHAM Emily [USTAN Partner 10] FIORINI Federica [HZI Partner 18] Project funded by the European Union’s Horizon 2020 research and innovation programme (2015-2019) Dissemination Level PU Public X PP Restricted to other programme participants (including the Commission Services) RE Restricted to a group specified by the consortium (including the Commission Services) CO Confidential, only for members of the consortium (including the Commission Services 2 Abstract Deliverable D6.1b replaces Deliverable 6.1 EMBRIC showcases: prototype pipelines from the microorganism to product discovery with the specific goal to refine technologies used but more specifically deliver results of the microbial discovery pipeline. -
Identification and Initial Characterization of Prophages in Vibrio Campbellii
RESEARCH ARTICLE Identification and Initial Characterization of Prophages in Vibrio campbellii Nicola Lorenz1, Matthias Reiger1¤a, Mauricio Toro-Nahuelpan2,3, Andreas Brachmann4, Lisa Poettinger1, Laure Plener1¤b, Jürgen Lassak1, Kirsten Jung1* 1 Munich Center for Integrated Protein Science (CiPSM) at the Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Martinsried, Germany, 2 Department of Microbiology, University of Bayreuth, Bayreuth, Germany, 3 Department of Molecular Structural Biology, Max-Planck-Institute of Biochemistry, Martinsried, Germany, 4 Department of Biology I, Genetics, Ludwig-Maximilians-Universität München, Martinsried, Germany ¤a Current address: Chair and Institute of Environmental Medicine, UNIKA-T, Technical University of Munich and Helmholtz Zentrum München–German Research Center for Environmental Health, Augsburg, Germany ¤b Current address: Gene & GreenTK, Faculté de Médecine, Marseille, France a11111 * [email protected] Abstract Phages are bacteria targeting viruses and represent the most abundant biological entities on earth. Marine environments are exceptionally rich in bacteriophages, harboring a total of OPEN ACCESS 4x1030 viruses. Nevertheless, marine phages remain poorly characterized. Here we Citation: Lorenz N, Reiger M, Toro-Nahuelpan M, describe the identification of intact prophage sequences in the genome of the marine γ-pro- Brachmann A, Poettinger L, Plener L, et al. (2016) Identification and Initial Characterization of teobacterium Vibrio campbellii ATCC BAA-1116 (formerly known as V. harveyi ATCC BAA- Prophages in Vibrio campbellii. PLoS ONE 11(5): 1116), which presumably belong to the family of Myoviridae. One prophage was found on e0156010. doi:10.1371/journal.pone.0156010 chromosome I and shows significant similarities to the previously identified phage ΦHAP-1. Editor: Baochuan Lin, Naval Research Laboratory, The second prophage region is located on chromosome II and is related to Vibrio phage UNITED STATES kappa.