Phylogeny of Bumble Bees in the New World Subgenus Fervidobombus (Hymenoptera: Apidae): Congruence of Molecular and Morphological Data
MOLECULAR PHYLOGENETICS AND EVOLUTION Molecular Phylogenetics and Evolution 28 (2003) 552–563 www.elsevier.com/locate/ympev Phylogeny of bumble bees in the New World subgenus Fervidobombus (Hymenoptera: Apidae): congruence of molecular and morphological data Sydney A. Camerona,* and Paul H. Williamsb a Department of Entomology, 320 Morrill Hall, 505 S. Goodwin Ave., University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA b Department of Entomology, The Natural History Museum, Cromwell Road, London SW7 5BD, UK Received 24 July 2002; revised 29 January 2003 Abstract We present new DNA sequence data (12S,16S,and opsin gene fragments) and morphological characters of the male genitalia for a phylogenetic analysis of the bumble bee subgenus Fervidobombus. There is no significant incongruence between the three molecular data sets,and little incongruence between the DNA and morphology. Simultaneous analysis of all the data partitions resulted in a tree that was entirely congruent with the All-DNA tree. Optimization of the geographic locations of the taxa onto the tree topology using dispersal/vicariance analysis suggests a complex picture of spread and diversification of Fervidobombus from the Old World into the southern New World. There is a phylogenetic component to their spread into tropical rain forest,as the two primary rain forest species (Bombus transversalis and Bombus pullatus) comprise a monophyletic clade,along with a third species, Bombus atratus, which is widely distributed in South America,including lowland subtropical habitats. Ó 2003 Elsevier Science (USA). All rights reserved. Keywords: Bombini; Molecular systematics; Morphology; Social insects; Neotropical; Biogeography; Dispersal/vicariance; DIVA 1. Introduction as far as the southern tip of Chile (Franklin,1913; Labougle,1990; Milliron,1973; map in Williams,1998).
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