bioRxiv preprint doi: https://doi.org/10.1101/2020.10.18.342550; this version posted October 19, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Gulf of Mexico blue hole harbors high levels of novel microbial lineages 2 3 Patin NV1,2*, Dietrich ZA3, Stancil A4, Quinan M4, Beckler JS4, Hall ER5, Culter J5, Smith 4 CG6, Taillefert M7, Stewart FJ1,2,8 5 6 1. School of Biological Sciences, Georgia Institute of Technology, Atlanta GA 30332 7 2. Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta 8 GA 30332 9 3. Bowdoin College, Brunswick ME 04011 10 4. Harbor Branch Oceanographic Institute, Florida Atlantic University, Ft. Pierce FL 11 34946 12 5. Mote Marine Laboratory, Sarasota FL 34236 13 6. St. Petersburg Coastal and Marine Science Center, United States Geological Survey, 14 St. Petersburg FL 33701 15 7. School of Earth & Atmospheric Sciences, Georgia Institute of Technology, Atlanta GA 16 30332 17 8. Department of Microbiology & Immunology, Montana State University, Bozeman MT 18 59717 19 20 * Corresponding author:
[email protected] 21 22 Abstract 23 24 Exploration of oxygen-depleted marine environments has consistently revealed novel 25 microbial taxa and metabolic capabilities that expand our understanding of microbial 26 evolution and ecology. Marine blue holes are shallow karst formations characterized by 27 low oxygen and high organic matter content. They are logistically challenging to sample, 28 and thus our understanding of their biogeochemistry and microbial ecology is limited.