ABSTRACT ORMOND, ROBERT BRYAN. Advancement in the Man
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ABSTRACT ORMOND, ROBERT BRYAN. Advancement in the Man-In-Simulant-Test Methodology and Development of Next Generation Manikin for Chemical and Biological Protection Research. (Under the direction of Dr. Roger L. Barker and Dr. Keith R. Beck). The Man-In-Simulant-Test (MIST) has been the primary method used to evaluate full ensembles with regards to their chemical protective performance since it was developed by the U.S. Army during the early 1990s. MIST evaluations involve exposing human test subjects wearing full protective ensembles to a low toxicity simulant for chemical warfare agents. The target property that is assessed by the MIST is the ensemble’s ability to prohibit or minimize inward leakage of the simulant through the closures and interfaces such as seams, seals, or zippers. The first area that was addressed by this research was the advancement of the current MIST methodology. A detailed extraction and analysis method was developed to remove the simulant, methyl salicylate (MeS), from the Passive Adsorbent Dosimeters (PADs) that are used to measure the leakage inside the ensemble. The removal of MeS from the adsorbent was shown to have an extraction efficiency greater than 98% across the entire expected range of MeS amounts. The liquid chromatography method was shown to be capable of detecting MeS in the expected ranges, provided an overall limit of quantitation of 30 ng/PAD, and only required 4.5 minutes to analyze a single sample. A bench-scale MIST chamber was developed to quickly expose multiple PADs or materials at various MeS concentrations. The chamber was shown to be capable of maintaining a desired concentration for an extended period of time and could also be repeatedly filled to the same conditions across multiple days. An extensive investigation was conducted to characterize the PADs based on the consistency of their physical properties and the effect of varying the exposure conditions on the measured uptake rate. Within a single lot, the PADs were shown to be relatively consistent in surface area and responded linearly to a range of exposure dosages. A significant variability between PAD lots was observed and should be further investigated. The PAD handling procedures were also investigated, and it is recommended to either extract the PADs within an hour of being exposed or store them at extremely cold temperatures to prohibit any mass transfer from occurring during storage. Various fabrics were investigated to determine their MeS uptake rates in both the bench-scale and full-scale MIST chambers. Fabrics comprised of 100 % polyester or cotton were shown to adsorb very little MeS, but incorporating only 5% spandex greatly increased both the capacity and uptake rate of the fabrics. A 95/5 polyester/spandex jersey fabric was found to have a thirty minute uptake rate very similar to the values reported for human skin, and therefore could potentially serve as a skin simulant in MIST protocols utilizing manikins. The second main area that was addressed by this research was the development of a next generation manikin for use in MIST evaluations. An articulated thermal manikin was attached to an elliptical walker to increase the range of motion. A baseline for the observed variability in MIST results with human subjects was determined by conducting three separate trials with eight human subjects in the MIST Facility at NC State University. These results were compared to three manikin evaluations in the same NFPA 1994 Class 3 Ensemble. It was found that manikins and human subjects can produce consistent MIST results. It was also confirmed that three manikin evaluations produce the just as much variability as three human subjects, and therefore should not be used to replace a standard eight human subject test. Advancement in the Man-In-Simulant-Test Methodology and Development of Next Generation Manikin for Chemical and Biological Protection Research by Robert Bryan Ormond A dissertation submitted to the Graduate Faculty of North Carolina State University in partial fulfillment of the requirements for the Degree of Doctor of Philosophy Fiber and Polymer Science Raleigh, North Carolina 2012 APPROVED BY: _______________________________ ________________________________ Donald B. Thompson Ronald Baynes Committee Member Minor Committee Member _______________________________ ________________________________ Roger L. Barker Keith R. Beck Chair of Advisory Committee Co-Chair of Advisory Committee DEDICATION First and foremost, this dissertation is dedicated to the memory of all the victims of the terrorist attacks on September 11, 2001, and to the soldiers, policemen, firefighters, and other first responders that put their lives in harm’s way each and every day to protect complete strangers. This dissertation is also dedicated to: • To my Grandfather, Ray Maready, for teaching me that in life, just as in checkers, if you don’t make a move you will get blown off the board, and also for showing me that no matter what odds you are given or what adversity you face, you must stay strong in your faith and never give up. • To my Grandmother, Louise, for showing me how to put others first and always take care of my family, and also for teaching me how to make one of the best pizzas you will ever taste. • To my Aunt, Yvonne, and her family for reminding me to always be prepared because you never know when you will need your electrical tape. • To my Uncle Phil, Aunt Angel, and their family for allowing me to experience the world of music and also the world of college football and basketball through a trumpet. • To my sisters, Lisa and Heather, for showing me what true inner strength looks like and how to keep going everyday no matter what stands in your way. • To my nieces, Hailey, Kylie, and Lily, and my nephews, Ethan and Josh, for letting me know how it feels to have someone look up to you. • To my mother, Juanita, for teaching me how to be compassionate, patient, and for molding me into the man that I have become. ii • To my father, Robert, for showing me how to stand firm in my beliefs and never compromise myself to satisfy other people. • To my wife, Sasha, for supporting me, listening when I needed to vent, and for allowing me to do the same for you as we have traveled this road side by side. • To my best friends, my boxer/bulldogs, Cotton and Chroma, who never cared what went right or wrong during the day or what I did or didn’t finish, but always met me at the door only expecting a scratch behind the ears. • Finally, to my entire family for believing in me and encouraging me to never give up and always push forward. I would be a shell of myself without the impact that each and every one of you have had on my life. iii BIOGRAPHY Robert Bryan Ormond was born to Robert and Juanita Ormond on December 12, 1984 in Williamston, NC. With his father being a minister in the Pentecostal Holiness Church, most of his childhood was spent in various parts of eastern North Carolina. He was raised alongside his two older sisters, Lisa and Heather. Bryan graduated as valedictorian of North Johnston High School in Kenly, NC in May of 2003. Following graduation, Bryan moved to Raleigh, NC to attend NC State University’s College of Textiles. As a student, Bryan was heavily involved with many organizations both in the College of Textiles and across the university. He spent two seasons playing trumpet for the Power Sound of the South Marching Band and four seasons as a member of the Wolfpack Basketball Pep Band. Bryan also served as the President of both the Phi Psi National Textile Fraternity and NCSU’s Student Chapter of the AATCC. When not engrossed in his studies, Bryan also worked as an intern at Cotton Incorporated for over three years. In 2007, Bryan graduated Summa Cum Laude with a B.S. in Polymer and Color Chemistry with an American Chemical Society certification. He was named the 2007 AATCC Outstanding College Graduate of the Year and also received both the College of Textiles Leadership Award and the Phi Psi Senior Award for his dedication to the college. After completion of his undergraduate degree, Bryan began pursuing his doctorate at the Center for Research on Textile Protection and Comfort at NC State. Bryan has presented his research on the Man-In-Simulant-Test at multiple conferences both domestic and international and will graduate with a PhD in Fiber and Polymer Science in May 2012. Bryan and his wife, Sasha, were married in August 2008 and now reside in Raleigh, NC with their two boxer/bulldogs, Cotton and Chroma. iv ACKNOWLEDGMENTS I would like to express my appreciation and gratitude to members of my research committee for the guidance that they provided over the past five years. I would like to thank Dr. Roger Barker for serving as my research advisor, allowing me to take my research in the direction that I believed it should go, and pushing me to become an expert in my field. I would also like to specifically thank Dr. Keith Beck for allowing me to learn from his extensive knowledge of analytical chemistry. The experience and techniques that I gained from the time spent with him will be invaluable as I progress into my future career. I would also like to acknowledge and thank all of the current and former members of the TPACC family for the support and guidance that they provided. I am grateful to Shawn Deaton, Don Thompson, Gail Liston, John Morton-Aslanis, Mark Martin, Kevin Ross, and Michelle Lucas. I am also thankful for my fellow graduate students, Lee Gladish, Ashley Bradham, Taylor O’Cain, Andrew White, Lauren Deuser, Jessica Watkins, and Marika Walker, for the countless hours that they put in to help conduct the MIST trials.