Laboratory Practicals in Physiology

Total Page:16

File Type:pdf, Size:1020Kb

Laboratory Practicals in Physiology Laboratory Practicals in Physiology Contents I. BLOOD................................................................................................................................................................4 1. Methods of blood sampling .............................................................................................................................4 2. Anticoagulants .................................................................................................................................................4 3. Blood typing and compatibility tests ...............................................................................................................5 4. Bleeding time ..................................................................................................................................................8 5. Clotting time ....................................................................................................................................................8 6. Prothrombin time (Quick-time) .......................................................................................................................9 7. Partial thromboplastin time (PTT, theoretically only) ................................................................................... 10 8. Thrombin time (theoretically only) ............................................................................................................... 11 9. Hematocrit ..................................................................................................................................................... 11 10. Erythrocyte sedimentation rate as measured by theWestergren method ..................................................... 11 11. Blood cells counts ....................................................................................................................................... 12 12. Osmotic fragility (resistance) of red blood cells.......................................................................................... 15 13. RBC's diameter (Price-Jones curve, in theory) ............................................................................................ 15 14. Staining index (in theory) ............................................................................................................................ 16 15. The blood smear (differential leukocyte count) .......................................................................................... 16 II. CIRCULATION AND RESPIRATION ....................................................................................................... 19 1. Experiments on the isolated rat heart preparation (Langendorf perfusion) ................................................... 19 2. Electrocardiography ...................................................................................................................................... 20 3. Characteristics of the arterial pulse ............................................................................................................... 24 4. Observation of pulsation of the external jugular vein ................................................................................... 25 5. Blood pressure measurement ......................................................................................................................... 25 6. Percussion over the chest .............................................................................................................................. 26 7. Auscultation over the heart and lung ............................................................................................................. 27 8. The effect of physical exercise on the circulation ......................................................................................... 28 9. Spirometry ..................................................................................................................................................... 28 10. Determination of the respiratory pressure ................................................................................................... 31 11. Effect of the breathing cycle on the circulation ........................................................................................... 31 12. The cold-pressor test ................................................................................................................................... 32 13. Triple response of the skin .......................................................................................................................... 32 III. EXCRETION ................................................................................................................................................. 33 1. The Color, Smell and Turbidity of Urine ...................................................................................................... 33 2. The pH of the Urine ...................................................................................................................................... 33 3. The Specific Gravity of the Urine ................................................................................................................. 33 4. Quantity of the Urine ..................................................................................................................................... 34 5. Microscopic Investigation of the Urine Sediment ......................................................................................... 34 6. Detection of UBG in Urine with Ehrlich's Aldehyde test. ............................................................................. 36 7. Detection of Calcium According to Sulkowitsch .......................................................................................... 36 8. Detection of Sugar According to Nylander and Fehling: .............................................................................. 37 9. Detection of Proteins ..................................................................................................................................... 37 10. Detection of Blood and its Decomposition Products with the Benzidine Test ............................................ 38 11. Detection of Acetone ................................................................................................................................... 39 12. Detection of Bile Pigment ........................................................................................................................... 40 13. Detection of pus with Donne's Test (principle) ........................................................................................... 40 14. Rapid Strip Tests ......................................................................................................................................... 40 15. The Effect of ADH on Rats ......................................................................................................................... 41 16. Dilution and Concentration Tests in Humans (principle) ............................................................................ 41 17. Counting Cellular Elements in Urine According to Addis's method ........................................................... 42 18. Determination of Clearance ......................................................................................................................... 42 IV. ALIMENTARY CANAL .............................................................................................................................. 44 1. The pH of Saliva ........................................................................................................................................... 44 2. The detection of proteins ............................................................................................................................... 44 3. Investigation of saliva-amylase and maltase ................................................................................................. 44 4. Obtaining Gastric Juice, investigation of the acidity of gatsric juice ............................................................ 44 5. Detection of lactic acid in the gastric juice (Uffelmann's reaction). .............................................................. 45 6. Investigation of the protein digesting function of pepsin in gastric content. ................................................. 45 V. CENTRAL NERVOUS SYSTEM ................................................................................................................. 46 1. Investigation of Blood-brain barrier and neurogen inflammation in the rat .................................................. 46 2 2. Tendon Reflex (synonyms: proprioceptive reflex, stretch reflex, monosynaptic reflex) .............................. 46 3. Skin Reflexes (synonyms: flexor reflex, protective reflex, polysynaptic reflex) .......................................... 47 4. Investigation of Tremor ................................................................................................................................. 48 5. Investigation of Reaction Time ..................................................................................................................... 48 6. Electroencephalogram (EEG) ........................................................................................................................ 49 VI. THE ORGANS OF SENSE ..........................................................................................................................
Recommended publications
  • Thomas Addis (1881—1949): Mixing Patients, Rats, and Politics
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Kidney International, Vol. 37 (1990), pp. 833—840 HISTORICAL ARCHIVE CARL W. GOi-FSCHALK, EDITOR Thomas Addis (1881—1949): Mixing patients, rats, and politics STEVEN J. PEITZMAN Department of Medicine, Division of Nephrology and Hypertension, The Medical College of Pennsylvania, Philadelphia, Pennsylvania, USA In the early decades of the twentieth century, with the threatat the Laboratory of the Royal College of Physicians of Edin- of epidemic infectious diseases already in decline, attentionburgh, one of Great Britain's pioneering medical research shifted to the chronic maladies: hypertension, atherosclerosis,enterprises, also supported by the Carnegie Trusts. obesity, cancer, diabetes—and nephritis, or Bright's disease. Ray Lyman Wilbur (1875—1949), dean of the young Stanford New chemical methods devised by Otto Folin (1867—1934) atMedical School in 1911, "thought it would be a good thing to Harvard and Donald D. Van Slyke (1883—1971) at the Rock-bring in a young scientist from Scotland if the right one could be efeller Institute Hospital empowered the investigation of renalfound who had been trained in German as well as British and metabolic disorders. Folin's colorimetric system provideduniversities, and who was likely to develop in some promising rapid measurement of creatinine, urea, and uric acid, while Vanfield of research" [3]. So Wilbur sent a cable of invitation to Slyke's gasometric analyses allowed quantification of urea andEdinburgh, and the young Scotsman accepted the unlikely total carbon dioxide. Also in the first decades of the twentiethposition: in 1911 Stanford was still a relatively isolated and century, the reform of medical schools provided new opportu-little-known medical school in San Francisco (the school moved nities for academic medical careers.
    [Show full text]
  • Simplified Quantitative Methods for Bacteriuria and Pyuria
    J Clin Pathol: first published as 10.1136/jcp.16.1.32 on 1 January 1963. Downloaded from J. clin. Path. (1963), 16, 32 Simplified quantitative methods for bacteriuria and pyuria JAMES McGEACHIE AND ARTHUR C. KENNEDY From the University Departments of Bacteriology and Medicine, Royal Infirmary, Glasgow SYNOPSIS Although pyelonephritis is a common disease, it escapes clinical detection in an un- desirably high proportion of patients. The present unsatisfactory diagnostic position would be much improved by widespread screening of patients by simple yet reasonably accurate methods. Bacterial counts by the pour-plate technique and estimates of the white cell excretion per hour or day, while undoubtedly of diagnostic value, are probably unsuitable for use on a wide scale. In an attempt to find more convenient procedures a simplified stroke-plate method of bacterial counting and a simplified quantitative white cell count method were devised and applied to over 1,000 mid-stream urine samples from 398 patients. Good correlation was obtained between the simpler stroke-plate method of bacterial counting and the more time-consuming pour-plate method. The quantitative white cell procedure was a much more sensitive index of pyuria than wet-film micro- scopy, and comparison with the bacterial count results showed that it gave a useful indication of urinary infection. It is suggested that a quantitative bacterial count should replace non-quantitativecopyright. culture methods when urinary infection is suspected and that the quantitative white cell count should be performed as a routine part of the initial clinical and laboratory assessment of all patients, followed by a bacterial count if pyuria is revealed.
    [Show full text]
  • Schedule of Benefits for Laboratory Services
    SCHEDULE OF BENEFITS For LABORATORY SERVICES April 1, 1999 LABORATORY MEDICINE PREAMBLE: SPECIFIC ELEMENTS In addition to the common elements, (see General Preamble to the Schedule of Benefits, Physician Services under the Health Insurance Act), all services listed under Laboratory Medicine from L001 to L699 (including L900 codes), L701 to L799 and under the "Laboratory Medicine in Private Office" listings in the Diagnostic and Therapeutic Procedures Section of the Schedule, when performed by a physician for his/her own patients, include the following specific elements: A. Carrying out the laboratory procedure, including collecting specimens where not separately billable, and processing of specimens. B. Interpreting and/or providing the results of the procedure, where not interpreted by a physician under an L800 code, even where the interpreting physician is another physician. C. Discussion with and providing advice and information to the patient or patient's representative(s), whether by telephone or otherwise, on matters related to the service. D. Providing premises, equipment, supplies and personnel for the specific elements and for any aspect(s) of the specific elements, of any service(s) covered by a corresponding L800 code that is (are) performed at the place in which the laboratory procedure is performed. OTHER TERMS AND DEFINITIONS 1. The patient documentation and specimen handling benefit (see code L700 below) is applicable to all insured procedures, except for those listed under anatomical pathology, histology and cytology, the fees for which cover any administrative cost. This benefit is not applicable to referred-in samples, since the collecting laboratory will already have claimed the patient documentation and specimen collection benefit.
    [Show full text]
  • HEMATURIA PADA ANAK Pasal 72 Undang-Undang Nomor 19 Tahun 2002 Tentang Hak Cipta
    HEMATURIA PADA ANAK Pasal 72 Undang-Undang Nomor 19 Tahun 2002 tentang Hak Cipta: (1) Barangsiapa dengan sengaja dan tanpa hak melakukan perbuatan sebagaimana dimaksud dalam Pasal 2 ayat (1) atau Pasal 49 ayat (1) dan ayat (2) dipidana dengan pidana penjara masing-masing paling singkat 1 (satu) bulan dan/atau denda paling sedikit Rp 1.000.000,00 (satu juta rupiah), atau pidana penjara paling lama 7 (tujuh) tahun dan/atau denda paling banyak Rp 5.000.000.000,00 (lima miliar rupiah). (2) Barangsiapa dengan sengaja menyiarkan, memamerkan, mengedarkan, atau menjual kepada umum suatu Ciptaan atau barang hasil pelanggaran Hak Cipta atau Hak Terkait sebagaimana dimaksud pada ayat (1) dipidana dengan pidana penjara paling lama 5 (lima) tahun dan/atau denda paling banyak Rp 500.000.000,00 (lima ratus juta rupiah). (3) Barangsiapa dengan sengaja dan tanpa hak memperbanyak penggunaan untuk kepentingan komersial suatu Program Komputer dipidana dengan pidana penjara paling lama 5 (lima) tahun dan/atau denda paling banyak Rp 500.000.000,00 (lima ratus juta rupiah). (4) Barangsiapa dengan sengaja melanggar Pasal 17 dipidana dengan pidana penjara paling lama 5 (lima) tahun dan/atau denda paling banyak Rp 1.000.000.000,00 (satu miliar rupiah). (5) Barangsiapa dengan sengaja melanggar Pasal 19, Pasal 20, atau Pasal 49 ayat (3) dipidana dengan pidana penjara paling lama 2 (dua) tahun dan/ atau denda paling banyak Rp 150.000.000,00 (seratus lima puluh juta rupiah). (6) Barangsiapa dengan sengaja dan tanpa hak melanggar Pasal 24 atau Pasal 55 dipidana dengan pidana penjara paling lama 2 (dua) tahun dan/atau denda paling banyak Rp 150.000.000,00 (seratus lima puluh juta rupiah).
    [Show full text]
  • Disorders of Urinary Tract
    DISORDERS OF URINARY TRACT {In March 1949 the Frank E. Bunts Educational Institute presented a continuation course on Diabetes, the proceedings of which were later published in the Cleveland Clinic Quarterly. These proved so popular that it has been decided to publish the proceedings of the similar course on Medical and Surgical Disorders of the Urinary Tract held on November 17, 18, and 19, 1949.—Ed.) PYELONEPHRITIS AND GLOMERULONEPHRITIS Robert D. Taylor, M.D. Both pyelonephritis and glomerulonephritis can cause proteinuria, pyuria, hema- turia, cylindruria, and eventually hypertension and renal failure. Glomerulonephritis has been more widely studied and hence, in spite of an incidence of only 0.7 per cent, is a more common diagnosis in the presence of abnormal urinary findings than is pye- lonephritis which was observed in 5.6 per cent of 3607 postmortems. Glomerulone- phritis is a relentlessly progressive disease while pyelonephritis can be cured or controlled. Clinical Differential Diagnosis A. Chronic Pyelonephritis B. Chronic Glomerulonephritis 1. History 1. History More common among women; often There is no sex predominance. There is follows marriage or pregnancy. Recent or sometimes history of acute hemorrhagic remote history of chills, fever with dysuria. glomerulonephritis. More often protein- Nocturia is more frequent during active uria is discovered during routine exam- phase. Edema is rare save in terminal ination. Edema is frequent. Nocturia state. gradually increases and becomes con- tinuous. 2. Physical Findings 2. Physical Findings Patients may appear acutely or chron- Unless the process is acute or terminal, ically ill. Blood pressure is usually normal patients appear well. Blood pressure is and becomes elevated only as renal failure often normal, commonly moderately ele- supervenes; occasionally acute hyper- vated early in course; high in later phases.
    [Show full text]
  • Others, That the Antistreptolysin Titer of the Serum Titers of 200 Units Or Over
    STUDIES OF THE VARIATIONS IN THE ANTISTREPTOLYSIN TITER OF THE BLOOD SERUM FROM PATIENTS WITH HEMORRHAGIC NEPHRITIS. II. OBSERVATIONS ON PATIENTS SUFFERING FROM STREPTOCOCCAL INFECTIONS, RHEUMATIC FEVER AND ACUTE AND CHRONIC HEMORRHAGIC NEPHRITIS By WARFIELD T. LONGCOPE 1 (From the Medical Clinic, the School of Medicine, Johns Hopkins University and Hospital, Baltimore) (Received for publication January 15, 1936) The observations recorded in the preceding sec- of the adult under normal conditions usually lies tion form a fairly satisfactory background for between 25 and 50 units and rarely exceeds 100 further investigations. In these studies, which units. Persons with chronic disease or acute in- may be regarded as controls, it was found that fections, not known to be associated with or com- the titer of the antistreptolysin of the serum plicated by hemolytic streptococcal infections, are varied within narrow limits from one normal per- more likely to show slightly higher titers than the son to another, 75 per cent giving titers between normal. 25 and 50 units. It was, in addition, noted that We have selected several forms of hemolytic the antistreptolysin titer remained fairly constant streptococcal infections for study. There are 19 in the same individual from month to month, pro- cases of erysipelas, 18 cases of scarlatina, 14 cases vided a hemolytic streptococcal infection did not of acute tonsillitis due to B. hemolytic strepto- supervene. The antistreptolysin in the serum cocci, and 7 cases of miscellaneous infections due from patients affected by a variety of chronic dis- to hemolytic streptococci, from two of which mi- eases behaved in much the same manner as in nute hemolytic streptococci were recovered in normal persons, for only 14 out of 84 patients pure culture by Dr.
    [Show full text]
  • 1. the Color, Smell and Turbidity of Urine 2. the Ph of the Urine 3. The
    V. EXCRETION 1. The Color, Smell and Turbidity of Urine The color of normal urine ranges from colorless through straw, yellow, amber and brown and depends on the concentration of various pigments (urochrome, uroerythrin, urobilin). The color of urine can be influenced by: - pH: acidic urine usually darker. - concentration: pale yellow urine is usually hypotonic, while dark urine is hypertonic (except in osmotic diuresis -e.g. in glucosuria- where the pale colored urine has high specific weight). The color of urine might change due to different substances or under pathologic conditions. Interpretation of atypical urine color is as follows: yellow/orange: concentrated urine, urobilinogen, bilirubin, red/red-brown: red blood cells, hemoglobin, myoglobin, phenolphtalein (laxative), blackberries, menstrual contamination, porphyrin, amidazophen brown-black/black: melanin, methemoglobin, homogentisic acid, (the homogentisic acid defect: homogentisic acid does not decompose, but rather is transformed into alkaptochrome while standing) fecal contamination blue-green: pseudomonas infection, chlorophyll, biliverdin, methylene blue, milky/cloudy: pyuria, lipids, mucus, radiographic dye, microorganisms The observation of turbidity or lack of it in a urine specimen is made by the examination of a well mixed, uncentrifuged sample held against a good light source. Terms: clear, hazy, cloudy, opaque. 2. The pH of the Urine The pH of the urine is depending on the acid-base equilibrium of the organism and may vary between pH 4 and 8. In the case of a normal mixed diet it is slightly acidic. Protein-rich diets shift the pH into the acidic range, while vegetables shift it towards a basic value. The pH of the urine can be determined simply with indicator paper: dip the reagent strip into the urine and remove immediately.
    [Show full text]
  • Laboratory Emerging Pathogens Initiative (Epi) Roll up Modifications Technical and User Manual
    LABORATORY EMERGING PATHOGENS INITIATIVE (EPI) ROLL UP MODIFICATIONS TECHNICAL AND USER MANUAL PATCH LR*5.2*281 Version 5.2 June 2004 Department of Veterans Affairs VistA Health System Design and Development Preface The Veterans Health Information Systems and Architecture (VistA) Laboratory Emerging Pathogens Initiative (EPI) Rollup Modifications Patch LR*5.2*281 Technical and User Manual provides assistance for installing, implementing, and maintaining the EPI software application enhancements. Intended Audience The intended audience for this manual includes the following users and functionalities: • Veterans Health Administration (VHA) facility Information Resource Management (IRM) staff (will be important for installation and implementation of this package) • Laboratory Information Manager (LIM) (will be important for installation and implementation of this package) • Representative from the Microbiology section in support of the Emerging Pathogens Initiative (EPI) Rollup enhancements (i.e., director, supervisor, or technologist) (will be important for installation and implementation of this package especially with parameter and etiology determinations; may also have benefit from local functionality) • Total Quality Improvement/Quality Improvement/Quality Assurance (TQI/QI/QA) staff or persons at the VHA facility with similar function (will be important for implementation of this package given broad-ranging impact on medical centers and cross-cutting responsibilities that extend beyond traditional service lines; may also have benefit from local functionality) • Infection Control Practitioner (likely to have benefit from local functionality) NOTE: It is highly recommend that the Office of the Director (00) at each VHA facility designate a person or persons who will be responsible for the routine implementation of this patch (both at the time of this installation and afterwards) and to take the lead in trouble-shooting issues that arise with the routine functioning of the process.
    [Show full text]
  • The Effect of the Toxemias of Pregnancy on Renal Function
    THE EFFECT OF THE TOXEMIAS OF PREGNANCY ON RENAL FUNCTION C. A. Elden, … , F. D. Sinclair Jr., W. C. Rogers J Clin Invest. 1936;15(3):317-322. https://doi.org/10.1172/JCI100781. Research Article Find the latest version: https://jci.me/100781/pdf THE EFFECT OF THE TOXEMIAS OF PREGNANCY ON RENAL FUNCTION By C. A. ELDEN, F. D. SINCLAIR, JR., AND W. C. ROGERS (From the Department of Obstetrics and Gynecology, the University of Rochester School of Medicine and Dentistry, Rochester, New York) (Received for publication February 5, 1936) In a previous contribution (1) it was shown RESULTS that there is a wider range of normal values for Preeclampsia (Table I) renal function tests in normal women in the last This group includes twenty cases of preeclamp- trimester of pregnancy than in normal non-preg- sia varying from the milder forms with elevated nant individuals. The blood urea clearance (2) blood pressure and albuminuria to those verging was shown to vary between 60 and 118 per cent on eclampsia. They are about equally divided be- of normal. The total protein content of the urine tween the two subgroups. There was no history was within normal limits. The urinary sediment of nephritis in any of the cases. In nine of the count of Addis (3) showed the casts to vary from twenty cases there was a history of a previous 0 to 10,000, the red blood cells to range from preeclampsia. The cases with a history of a pre- 47,000 to 1,900,000 and the white blood and epi- vious toxemia are about equally divided between thelial cells to vary from 25,000 to 6,000,000.
    [Show full text]
  • A History of Urine Microscopy
    Clin Chem Lab Med 2015; 53(Suppl): S1453–S1464 Review J. Stewart Cameron* A history of urine microscopy DOI 10.1515/cclm-2015-0479 Keywords: history; microscopy; renal disease; urinary Received March 17, 2015; accepted March 30, 2015; previously sediment. published online June 16, 2015 Abstract: The naked-eye appearance of the urine must “When the patient dies the kidneys may go to the pathologist, but have been studied by shamans and healers since the Stone while he lives the urine is ours. It can provide us day by day, month by month, and year by year with a serial story of the major events within Age, and an elaborate interpretation of so-called Uroscopy the kidney. The examination of the urine is the most essential part of began around 600 AD as a form of divination. A 1000 years the physical examination of any patient...” (Thomas Addis, 1948 [1]). later, the first primitive monocular and compound micro- scopes appeared in the Netherlands, and along with many other objects and liquids, urine was studied from around Urine examination before micros- 1680 onwards as the enlightenment evolved. However, the crude early instruments did not permit fine study because copy and chemistry: the stone age of chromatic and linear/spherical blurring. Only after to 1580 complex multi-glass lenses which avoided these problems had been made and used in the 1820s in London by Lis- From the earliest days of shamans and healers in the Pal- ter, and in Paris by Chevalier and Amici, could urinary aeolithic era, perhaps 30–40,000 years or more ago, the microscopy become a practical, clinically useful tool in urine coming out of the body has been examined by the the 1830s.
    [Show full text]
  • Appendix I: Reference Intervals
    Appendix I: Reference Intervals AMIN Y. BARAKAT The following reference intervals are guidelines usually needed in the diagnosis and management of renal disease. Values may vary according to the methodology used. Laboratory values are given in conventional and international units. A few references have been used in the formu­ lation of this table (1-5); values from other sources have been referenced separately. The prefixes for units are the standard ones approved by the Conference Generale des Poids et Mesures, 1964, the International Union of Pure and Applied Chemistry and the International Federation of Clin­ ical Chemistry. Few other abbreviations are used: B, blood; Ca, calcium; d, day; EDT A, ethylenediaminetetraacetate, edetic acid; f, female; hr, hour; m, male; min, minute; mo, month; RBC, red blood cell; P, plasma; S, serum; U, urine; wk, week; yr, year. Conventional International Test Specimen units units g/dl giL Albumin S Premature 3.0-4.2 30-42 NB 3.6-5.4 36-54 Infant 4.0-5.0 40-50 Thereafter 3.5-5.0 35-50 U Look under urine protein ng/dl nmoljL Aldosterone P (heparin, EDT A) Newborn: 5-60 0.14-1.7 S I wk-I yr: 1-160 0.03-4.4 1-3 yr: 5-60 0.14-1.7 3-5 yr: <5-80 <0.14-2.2 5-7 yr: <5-50 <0.14-1.4 7-11 yr: 5-70 0.14-1.9 II-IS yr: <5-50 <0.14-1.4 414 Amin Y. Barakat Conventional International Test Specimen units units ngJdl nmol/L Adult: Average sodium diet Supine: 3-10 0.08-0.3 Upright F: 5-30 0.14-0.8 M: 6-22 0.17-0.61 2-3 X higher during pregnancy Adrenal vein: 200-800 ngJdl 5.5-22 Low sodium diet: increases 2-5 fold Florinef
    [Show full text]
  • FUNCTIONAL ASPECTS of the DIFFERENTIAL DIAGNOSIS of RENAL DISEASE JAN BROD, MI.D., D.Sc
    Postgrad Med J: first published as 10.1136/pgmj.39.449.121 on 1 March 1963. Downloaded from POSTGRAD. MED. J. (I963), 39, 12I FUNCTIONAL ASPECTS OF THE DIFFERENTIAL DIAGNOSIS OF RENAL DISEASE JAN BROD, MI.D., D.Sc. Director, Institute for Cardiovascular Researc3t, Prague 4, Czechoslovakia IF we read through both the basic papers which process, which might be present only in other William Bright wrote some 136 and 127 years ago, portions of the organ. In chronic pyelonephritis, and which laid the foundations of clinical nephro- for example, we can find quite normal areas of logy, we can recognize in the detailed clinical kidney in close approximation to areas severely description the nephrotic syndrome, the effects altered by the chronic process. of severe hypertension and the signs of chronic However, morphological criteria are but one renal or cardiac insufficiency. These are the end- consequence of the disease-process-the other stages which follow almost all types of chronic consequence consisting of the renal functional renal disease, and these late phenomena cannot be changes, which are no less specific than the used to differentiate between the various morpho- details of micro-morphology. Our own work, logical entities which the pathologists later con- and that of others, has led us to the conclusion structed from the original tissue of ' Bright's that a study of renal function can give us a great disease '. Such a differentiation was, of course, to deal of information not only concerning the degree no purpose at the period when therapeutic of the disease process-hitherto its main applica-by copyright.
    [Show full text]