Biological Activities and Phenolic Contents of Argania Spinosa L (Sapotaceae) Leaf Extract

Total Page:16

File Type:pdf, Size:1020Kb

Biological Activities and Phenolic Contents of Argania Spinosa L (Sapotaceae) Leaf Extract Dakiche et al Tropical Journal of Pharmaceutical Research December 2016; 15 (12): 2563-2570 ISSN: 1596-5996 (print); 1596-9827 (electronic) © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved. Available online at http://www.tjpr.org http://dx.doi.org/10.4314/tjpr.v15i12.5 Original Research Article Biological activities and phenolic contents of Argania spinosa L (Sapotaceae) leaf extract Hadjira Dakiche1,2*, Mustapha Khali1, Ahmed Khaled Abu-el-Haija3, Ammar Al- Maaytah3 and Qosay Ali Al-Balas3 1Laboratoire de Protection et de Valorisation des Ressources Agro-Biologiques, Faculté de Sciences de la Nature et de Vie, Université Saâd Dahlab Blida, BP 270, Route de Soumaâ, Blida 09000, 2Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques, BP 248, Alger RP, 16004 Alger, Algeria, 3Faculty of Pharmacy, Jordan University of Science and Technology, PO Box 3030, Irbid 22110, Jordan *For correspondence: Email: [email protected]; Tel: +213 555 497 211 Received: 20 June 2016 Revised accepted: 16 November 2016 Abstract Purpose: To investigate the phenolic profile and biological activities of Argania spinosa L. leaves. Methods: The crude methanol extract of leaves of A. spinosa L. Skeels was obtained by ultrasonic extraction, and the total polyphenolic and flavonoid contents were determined by ultra-high performance liquid chromatography-electrospray ionization-quadrupole time-of-flight-mass spectrometry (UPLC-ESI- QTOF-MS). In vitro antioxidant activity was determined by 2,2-di-phenyl-1-picryl-hydrazil (DPPH) radical assay and antimicrobial activity evaluated using agar disk diffusion method against reference pathogenic strains (Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 14579, Yersinia enterocolitica ATCC 23715, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922). Cytotoxic activity was evaluated by methyl-thiazolyldiphenyl-tetrazolium bromide (MTT) assay. Results: The results revealed abundant polyphenols and flavonoids (221.39 ± 5.70 µg GAEq/1 g and 66.86 ± 3.36 µg CAEq/1 g, respectively) in the leaf extract. UPLC-DAD-ESI-QTOF-MS profiling showed the presence of myrecitin-3-galactoside, myrecitin-3-gluctoside, myrecitin-3-xyloside, quercetin-3- galactoside, quercetin-3-glucoside, quercetin-3-arabinofuranoside, and quercetin-3-rhamnoside. DPPH assay of the leaf extract yielded a half-maximal effective concentration (EC50) value of 125.60 ± 1.87 µg. A. spinosa L. leaves exhibited antibacterial activity against Gram (+) and Gram (-) bacteria, with particularly marked activity against B. subtilis (inhibition zone, 16 mm). Cytotoxicity data showed that the extract inhibited the proliferation of PC3 cells (IC50 ~ 600 µg). Conclusion: A. spinosa L. leaf extract is rich in valuable biologically active compounds and could represent a new resource for natural and preventive therapies. Keywords: Argania spinosa, Polyphenols, Flavonoids, Antioxidant, Antibacterial, Cytotoxic Tropical Journal of Pharmaceutical Research is indexed by Science Citation Index (SciSearch), Scopus, International Pharmaceutical Abstract, Chemical Abstracts, Embase, Index Copernicus, EBSCO, African Index Medicus, JournalSeek, Journal Citation Reports/Science Edition, Directory of Open Access Journals (DOAJ), African Journal Online, Bioline International, Open-J-Gate and Pharmacy Abstracts INTRODUCTION [1]. This species is the most original essence of North Africa and is endemic to Algeria, growing Algeria has a diverse collection of flora, the in the southwest of Tindouf Province, from the majority of which may have therapeutic potential. Jebel Ouarkziz to Hamada, and in south-western Argania spinosa L., also known as the Argan Morocco [2]. This tree has been successfully tree, belongs to the Sapotaceae family and cultivated in some regions of northern Algeria, represents the only species of the genus Argania Trop J Pharm Res, December 2016; 15(12): 2563 Dakiche et al such as Stidia (Mostaganem Province), Chlef, method [6]. Gallic acid was used as the standard and Mascara. to generate a calibration curve, and measurements were carried out at 725 nm using Due to the presence of Argan oil, which has, for a UV-vis spectrophotometer (Shimadzu UV centuries, been used in the traditional treatment 1650, Italy). The results were expressed as of rheumatism, constipation, diabetes, and milligrams of gallic acid-equivalent per gram dry respiratory difficulties, as well as wounds and dry mass (mg GAE/g dry mass). skin, the seed kernel is the most valuable part of A. spinosa L. [3]. To date, the use of by-products Total flavonoids were estimated according to the or parts of Argan trees other than the kernel has method of Grand et al [7]. Quercetin was used as been limited (e.g., Argan leaves are used only in the standard to generate the calibration curve, livestock feed). This represents an economic loss and measurements were carried out at 510 nm due to its content of bioactive compounds. using a UV-vis spectrophotometer (Shimadzu UV Several phenolic compounds have been 1650, Italy). Results were expressed as detected in A. spinosa L. leaves from Morocco; milligrams of quercetin-equivalent per gram dry the main constituents are quercetin, hyperoside, mass (mg QAE/g dry mass). and myricetin [4], which have antioxidant, antimicrobial, and cytotoxic activities [5]. Polyphenol characterization by UPLC–ESI– QTOF–MS The present study was conducted to investigate and explore the potential of Argan leaves as a Polyphenolic compounds were identified using by-product, analyze phenolic compounds using an Acquity Ultra-Performance LC system (UPLC) UPLC-DAD-ESI-QTOF-MS and assess the coupled to a XEVO-G2-QTOF (quadrupole time- biological activities and pharmaco-toxicological of-flight) mass spectrometer (Waters properties (e.g., antioxidant, antibacterial, and Corporation, Manchester, United Kingdom), cytotoxic effects) of leaves of the Argan tree. equipped with an electrospray ionization (ESI) source operating in positive and negative modes, EXPERIMENTAL using the binary solvent manager software (Waters Corporation, UK). Plant materials Chromatographic separation was carried out on A. spinosa L. leaves were collected in the Stidia a UPLC HSS T3, C18 column (1.8 μM, 2.1 × 100 o region, Mostaghanem, north-western Algeria mM, Waters Corporation) at 30 C. The mobile (latitude 35°48’N, longitude 0°03’E; altitude 35 phase comprised Solvent (A) (0.1 % formic acid, m) from June to July 2014. The authentication v/v) and Solvent (B) (100 % methanol). The was performed by Professor H. Abdelkrim gradient used was 0–5 min, 0–10 % B; 5–10 min, (National Hight School of Agronomy, Algiers, 10–15 % B; 10–15 min, 15-20 % B; 15–20 min, Algeria). The voucher specimen was deposited in 20–30 % B; 20–30 min, 30-40 % B; and 30–35 the herbarium of Laboratory of Protection and min, 40-0 % B. The solvent flow rate was 0.4 Valorization of Agro-biological Resources, mL/min. The injection volume was 2 μL. University Saad Dahleb Blida, Algeria. The leaves were air-dried, powdered, and conserved Analysis was performed at a wavelength of 280 until used. nm, data depending MS scanning from 50 to 1500 m/z. Analysis was performed in positive Preparation of extract and negative ion modes using an electrospray source with a source temperature of 120 °C and Powdered A. spinosa L. leaves (20 g) were a desolvation temperature of 500 °C, a capillary defatted using petroleum ether (60 – 80 °C) and voltage of 1 kV, and a sampling cone voltage of then extracted with 200 ml of methanol/water (70/30, v/v) in an ultrasonic bath for 30 min. The 30 eV. Highly pure N2 (˃ 99.9995 %) was used solvent was then evaporated, and the extract as the desolvation gas (flow rate, 10 L/h) and was obtained with a yield of 13 %. The extract cone gas (flow rate, 1000 L/H). was stored in the dark at -20 °C until use. Chemical analysis Data were acquired using the MassLynx software (version 4.1, Waters). The exact masses and MS Total polyphenol and flavonoid determination fragmentation data were compared with those in the respect for phytochemicals and Mass Bank The total phenolics in the methanolic extract metabolomics data banks and in previous reports were determined by the Folin and Ciocalteu to identify phenolic compounds. Trop J Pharm Res, December 2016; 15(12): 2564 Dakiche et al Antioxidant assay Cytotoxicity assay DPPH assay of the methanolic extract of leaves The PC-3 human prostate cancer cell line was of A. spinosa L was carried out as described by used (ATCC). Cells were plated in RPMI 1640 Blois [8]. Dried extract was weighed and medium supplemented with 10 % FBS and 1 % reconstituted to known concentrations. The penicillin-streptomycin. All cultures were extract (0.3 mL) and a methanolic solution of incubated at 37 °C in a humidified atmosphere containing 5 % CO2. Cells were plated at 5.0 × DPPH (2.7 mL; 5 mg in 100 mL methanol) were 3 mixed and incubated at room temperature for 1 h 10 /well in 96-well plates and allowed to attach in the dark. The absorbance at 515 nm was then for 24 h. Cells were then treated for 24 h under measured (Shimadzu UV 1650, Italy). A control the conditions described above in the presence of 50–1000 μg/mL plant extract dissolved in solution was prepared with 0.3 mL methanol and DMSO followed by filtration through a 0.22-μm 2.7 mL methanolic solution of DPPH. Ascorbic syringe filter. Stock solutions were further diluted acid and Trolox were used as reference in culture medium to the appropriate compounds. Radical scavenging activity is concentrations. DMSO (1 %) functioned as a expressed as the percentage inhibition of DPPH vehicle control and had no effect on cell growth (D) as in Eq 1. [10]. D (%) = {(Ac – At)/Ac}100 …………… (1) The methyl-thiazolyldiphenyl-tetrazolium bromide (MTT) assay, which is based on conversion of where Ac and As are the absorbance of control the yellow tetrazolium salt-MTT to purple and test samples, respectively.
Recommended publications
  • Factors Affecting Invasive Species Abundance: the Barbary Ground Squirrel on Fuerteventura Island, Spain Marta López-Darias1,* and Jorge M
    Zoological Studies 47(3): 268-281 (2008) Factors Affecting Invasive Species Abundance: the Barbary Ground Squirrel on Fuerteventura Island, Spain Marta López-Darias1,* and Jorge M. Lobo2 1Departamento de Biología Aplicada, Estación Biológica de Doñana (CSIC), Pabellón del Perú, Avenida María Luisa s/n, Sevilla, E-41013, Spain 2Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales (CSIC), Calle José Gutiérrez Abascal 2, Madrid, E-28006, Spain. E-mail:[email protected] (Accepted November 14, 2007) Marta López-Darias and Jorge M. Lobo (2008) Factors affecting invasive species abundance: the Barbary ground squirrel on Fuerteventura Island, Spain. Zoological Studies 47(3): 268-281. We assessed the determinants of habitat selection by the Barbary ground squirrel (Atlantoxerus getulus) at Fuerteventura (Canary Is., Spain). We implemented general linear model (GLM) procedures to analyze the relationships between squirrel abundances and 4 kinds of variables related to the biological requirements of the species (environment, food resources, biotic interactions, and refuge/shelter). We performed a variance partitioning analysis between the most explicative categories to explore correlation patterns. The time of year and weather conditions of the census clearly influenced the number of individuals observed. Shelter variables were the best correlates of both the abundance of squirrels and the number of their scat. Although food resources were less important, the presence of certain plant species was correlated with squirrel abundance, while general environmental variables and interactions with other mammals did not affect its distribution. These results improve our understanding of the ecology and the establishment of this highly successful introduced species, providing basic knowledge for use with future management strategies in the Canarian Archipelago.
    [Show full text]
  • Algeria / February 2020
    FACT SHEET > Algeria / February 2020 ALGERIA February 2020 UNHCR is providing international UNHCR provides protection and UNHCR leads the provision of protection, assistance and assistance to refugees and humanitarian assistance to services to Sahrawi refugees in asylum-seekers in urban areas. Sahrawi refugees, which the five camps near Tindouf in In 2019, 109 refugees departed involves coordination with all south-western Algeria. for resettlement to third countries. humanitarian actors in Tindouf. FUNDING USD 37.4 M requested for Algeria Operation received as of 10 March 2020: Funded 12% USD 4.6 M Shelter remains a critical need for Sahrawi families. Unfunded 88% © UNHCR / R. Fraser USD 32.7 M UNHCR PRESENCE Staff: 48 National Staff 10 International Staff 2 National UNVs Offices: 1 Representation in Algiers and 1 Sub-Office in Tindouf UNHCR partner Danish Refugee Council ’s business network workshop in Boujdour camp, attended by 18 Sahrawi refugees representing 16 businesses. © DRC / M. Nassima Aissaoui www.unhcr.org 1 FACT SHEET > Algeria / February 2020 Working with Partners ■ UNHCR is a member of the United Nations Country Team and works closely with the Algerian Government and with 13 partners in Algeria, three in Algiers and ten in Tindouf. UNHCR’s partners are: Algerian Red Crescent (ARC), Association des Femmes Algériennes pour le Développement (AFAD), Asociación de Trabajadores y Técnicos sin Fronteras (ATTSF), Danish Refugee Council (DRC), Humanité et Inclusion (HI), Enfants Refugiés du Monde (ERM), Green Tea Association, Movement for Peace, Disarmament & Liberty (MPDL), Oxfam, Réseau algérien pour la défense des droits de l'enfant (NADA), Solidaridad Internacional Andalucía (SI-A), Triangle Génération Humanitaire (TGH) and Universidad Autonoma de Madrid (UAM).
    [Show full text]
  • Ethnoeconomical, Ethnomedical, and Phytochemical Study of Argania Spinosa (L.) Skeels: a Review
    ELSEVIER - Journal of Ethnopharmacology Review Article Ethnoeconomical, Ethnomedical, and Phytochemical Study of Argania spinosa (L.) Skeels: A Review. Zoubida Charrouf Dominique Guillaume ABSTRACT. Populations of Morocco South-western part traditionally use the fruits of Argania spinosa (L.) Skeels to prepare an edible oil whose obtention also furnishes, as side product, a cake used to feed the cattle and complements the forage furnished by the leaves of this same plant. Unfortunately, the wood of Argania spinosa is also used for fuel and deforestation is subsequently accelerated since populations are generally eager to replace argan groves by cultures of higher and immediate benefit. However, argan tree, that is particularly well adapted to grow in arid lands, has been proposed by several agencies to slow down the desert progress in northern Africa. In order to incite the South-western Morocco dwellers to reintroduce argan trees, a program aimed to increase the industrial value of Argania spinosa, and beginning by its phytochemical study, is currently carried out in Morocco. The results of these recent studies together with previous knowledge are summarised in this review. KEYWORDS. Morocco, Argania spinosa, argan tree, argan oil, ethnopharmacology, phytochemistry, saponins INTRODUCTION Argan tree (Argania spinosa (L.) Skeels), of the family sapotaceae, is endemic in South- western Morocco where it grows over about 320,000 square miles. For centuries, this slow growing and spiny tree, that may be either shrubby or up to seven or ten meters has played an essential ecological function in this part of Morocco. Indeed, it effectively protects the soil against heavy rain or wind-induced erosion and, furthermore, by shading all kind of cultures, maintains soil fertility.
    [Show full text]
  • Comparative Study of Argan and Olive Fruits and Oils
    435 Comparative study of argan and olive fruits and oils Dalila Demnati1, Sebastián Sánchez2, Rafael Pacheco2, Mohamed Zahar1 & Leopoldo Martínez2 1- Institut Agronomique et Vétérinaire Hassan. BP 6202, 10101, Rabat, Morocco. Email: [email protected] 2- Universidad de Jaén, Campus Las Lagunillas 23071 Jaén, Spain. Email: [email protected] Abstract This study was conducted to compare argan and olive fruits and virgin oils. Dry argan fruits, traditional and semiautomatic extracted argan oils, from roasted and unroasted seeds, from Essaouira’s area, were studied. Morphological characteristics of argan fruit were determined and compared with the ‘Picual’ olive’s ones. The results showed certain similarities between the two fruits. The quality parameters analyzed were acidity and peroxide value, K270, K232 and ΔK, total phenols and oil stability, comparing them with those of ‘Picual’ virgin olive oil. Quality parameters corresponded to the Moroccan Standard for edible virgin argan oil. Traditional argan oil showed the lowest stability whereas semiautomatic edible oil presented the highest one. However, virgin olive oil showed higher phenol content and better oxidative stability than the virgin argan oils. Keywords: Argan, Olive, Fruit, Quality Parameters, Phenols, Oil Stability. Etude comparative des caractéristiques des fruits et des huiles d’argan et d’olive Résumé Cette étude a pour but de comparer le fruit et l’huile d’argan et d’olive vierges. Les fruits secs d’arganier, les huiles d’argan obtenues par un procédé traditionnel ou semi-automatique, à partir d’amandons torréfiés ou non, de la région d’Essaouira, ont été étudiés. Les caractéristiques morphologiques du fruit de l’arganier ont été déterminées et comparées à ceux de l’olive, variété ‘Picual’.
    [Show full text]
  • ISTA List of Stabilised Plant Names 7Th Edition
    ISTA List of Stabilised Plant Names 7th Edition ISTA Nomenclature Committee Chair Dr. M. Schori Published by All rights reserved. No part of this publication may be The International Seed Testing Association (ISTA) reproduced, stored in any retrieval system or transmitted in Richtiarkade 18, CH- 8304 Wallisellen, Switzerland any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without prior ©2021 International Seed Testing Association (ISTA) permission in writing from ISTA. ISBN 978-3-906549-77-4 Valid from: 16.06.2021 ISTA List of Stabilised Plant Names 1st Edition 1966 ISTA Nomenclature Committee Chair: Prof P. A. Linehan 2nd Edition 1983 ISTA Nomenclature Committee Chair: Dr. H. Pirson 3rd Edition 1988 ISTA Nomenclature Committee Chair: Dr. W. A. Brandenburg 4th Edition 2001 ISTA Nomenclature Committee Chair: Dr. J. H. Wiersema 5th Edition 2007 ISTA Nomenclature Committee Chair: Dr. J. H. Wiersema 6th Edition 2013 ISTA Nomenclature Committee Chair: Dr. J. H. Wiersema 7th Edition 2019 ISTA Nomenclature Committee Chair: Dr. M. Schori 7th Edition 2 ISTA List of Stabilised Plant Names Table of Contents A .............................................................................................................................................................. 7 B ............................................................................................................................................................ 21 C ...........................................................................................................................................................
    [Show full text]
  • Argania Spinosa)
    plants Article Isolation and Functional Analysis of a PISTILLATA-like MADS-Box Gene from Argan Tree (Argania spinosa) Marwa Louati 1 , Blanca Salazar-Sarasua 2 , Edelín Roque 2, José Pío Beltrán 2, Amel Salhi Hannachi 1 and Concepción Gómez-Mena 2,* 1 Faculty of Sciences of Tunis, Campus Farhat Hached El Manar, University of Tunis El Manar, Tunis 2092, Tunisia; [email protected] (M.L.); [email protected] (A.S.H.) 2 Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universitat Politècnica de València, 46022 Valencia, Spain; [email protected] (B.S.-S.); [email protected] (E.R.); [email protected] (J.P.B.) * Correspondence: [email protected] Abstract: Argan trees (Argania spinosa) belong to a species native to southwestern Morocco, playing an important role in the environment and local economy. Argan oil extracted from kernels has a unique composition and properties. Argan trees were introduced in Tunisia, where hundreds of trees can be found nowadays. In this study, we examined reproductive development in Argan trees from four sites in Tunisia and carried out the functional characterization of a floral homeotic gene in this non-model species. Despite the importance of reproductive development, nothing is known about the genetic network controlling flower development in Argania spinosa. Results obtained in several plant species established that floral organ development is mostly controlled by MADS-box genes and, in particular, APETALA3 (AP3) and PISTILLATA (PI) homologs are required for proper petal and stamen identity. Here, we describe the isolation and functional characterization Citation: Louati, M.; Salazar-Sarasua, B.; Roque, E.; Beltrán, J.P.; Salhi of a MADS-box gene from Argania spinosa.
    [Show full text]
  • Facts and Misconceptions on the Palaearctic Existence of the Striped
    Mammalia 2017; aop Boris Kryštufek, Cătălin Stanciu, Danijel Ivajnšič*, Sidi Imad Cherkaoui and Franc Janžekovič Facts and misconceptions on the Palaearctic existence of the striped ground squirrel https://doi.org/10.1515/mammalia-2017-0060 echo the exclusive ecological requirements of species Received May 26, 2017; accepted July 27, 2017 and their evolutionary history in response to past eco- logical and geological processes (Lomolino et al. 2006). Abstract: The striped ground squirrel has a wide distri- Species’ ranges can be studied at various temporal and bution in the Ethiopian region but is restricted to a small spatial scales provided they are already documented. isolated area in Palaearctic Africa. This fragment was first Before a distributional map can be produced, data on recorded in the late 1940s in the Souss Valley (Morocco), spatial occurrence must be collected in the field. Despite however, not a single new observation has been published its obvious simplicity, field work constitutes a crucial step in the following decades. In September 2016 we surveyed and affects the consistency of analyses which may depend the Souss Valley and found squirrels at 43 sites within upon sophisticated tools and concepts. Incomplete or the triangle between Agadir–Taroudant–Tiznit. Occupied misleading distributional data will unavoidably compro- sites were not distributed at random but occurred between mise subsequent analyses and assessments. an altitude of 45–254 m and on a substrate with coarse tex- In this study we have addressed the only Palaearctic ture containing >65% sand. The vast majority of the sites occurrence of the striped ground squirrel Euxerus with squirrels (69%) were classified as suburban, culti- erythropus (Geoffroy Saint-Hilaire 1803) (formerly Xerus vated or both.
    [Show full text]
  • CIRCULAR Issue No
    FDACS-P-01915 CIRCULAR Issue No. 40 | October 2018 Florida Department of Agriculture and Consumer Services Division of Plant Industry The Buckthorns (Genus Sideroxylon): An Underappreciated Group of Florida Native Plants Paul T. Corogin; Bureau of Entomology, Nematology and Plant Pathology [email protected] or 1-888-397-1517 INTRODUCTION Tucked away amongst the rich diversity of Florida plant life surrounding us, one plant group can easily escape our notice: the genus Sideroxylon, belonging to the pantropical family Sapotaceae (sapodilla family). This circular will introduce the Sideroxylon species native to North America, featuring in detail species adapted to the temperate zone that may be of interest to the southern United States (U.S.). Some are endangered in Florida, and some are Florida endemics. Certain species have landscaping potential, but have long been ignored, but a few species are occasionally available from native plant nurseries (Betrock’s Plant Search 2018; FNPS 2018). Species of Sideroxylon attract pollinators when blooming, and birds and wildlife when fruiting; thus, they can be desirable additions to any Florida landscape. Sapotaceae are recognized by the presence of milky sap, brownish T-shaped hairs, fasciculate inflorescences (flowers in a bundle) and seeds with a large scar at one end (Pennington 1990, 1991). This woody family makes a large contribution to tropical plant biodiversity, being a major floristic component of tropical lowland wet forests in the Americas, Asia, Africa and the Pacific Islands (Gentry 1988). Sapotaceous plants are also economically important to humans. “Sapote” comes from the Nahuatl word meaning sweet fruit; most species bear such a fruit (e.g., the sapodilla and mamey sapote) (Smith et al.
    [Show full text]
  • Download Download
    BORN IN THE MEDITERRANEAN: Alicia Vicente,3 Ma Angeles´ Alonso,3 and COMPREHENSIVE TAXONOMIC Manuel B. Crespo3* REVISION OF BISCUTELLA SER. BISCUTELLA (BRASSICACEAE) BASED ON MORPHOLOGICAL AND PHYLOGENETIC DATA1,2 ABSTRACT Biscutella L. ser. Biscutella (5 Biscutella ser. Lyratae Malin.) comprises mostly annual or short-lived perennial plants occurring in the Mediterranean basin and the Middle East, which exhibit some diagnostic floral features. Taxa in the series have considerable morphological plasticity, which is not well correlated with clear geographic or ecologic patterns. Traditional taxonomic accounts have focused on a number of vegetative and floral characters that have proved to be highly variable, a fact that contributed to taxonomic inflation mostly in northern Africa. A detailed study and re-evaluation of morphological characters, together with recent phylogenetic data based on concatenation of two plastid and one nuclear region sequence data, yielded the basis for a taxonomic reappraisal of the series. In this respect, a new comprehensive integrative taxonomic arrangement for Biscutella ser. Biscutella is presented in which 10 taxa are accepted, namely seven species and three additional varieties. The name B. eriocarpa DC. is reinterpreted and suggested to include the highest morphological variation found in northern Morocco. Its treatment here accepts two varieties, one of which is described as new (B. eriocarpa var. riphaea A. Vicente, M. A.´ Alonso & M. B. Crespo). In addition, the circumscriptions of several species, such as B. boetica Boiss. & Reut., B. didyma L., B. lyrata L., and B. maritima Ten., are revisited. Nomenclatural types, synonymy, brief descriptions, cytogenetic data, conservation status, distribution maps, and identification keys are included for the accepted taxa, with seven lectotypes and one epitype being designated here.
    [Show full text]
  • Second International Training Courses on Marine Molecular Taxonomy” October 18 - 25, 2014 Mostaganem-ALGERIA
    Marine Molecular Taxonomy” Second International October 18 - 25, 2014 Training Courses on Mostaganem-ALGERIA 2014 Inter-Islamic Science and Technology Network on Oceanography, Izmir - TURKEY University of Abdelhamid Ibn Badis Mostaganem - ALGERIA University of Oran Es-Sénia- ALGERİA National High School of Marine Sciences & Coastal Management - ALGERIA AN OVERVIEW OF THE PROGRAM University of KwaZulu-Natal-SOUTH AFRICA University of Annaba, ALGERIA INOC- Mostaganem University September 2014 IDB Second International Training Courses on Marine Molecular Taxonomy” October 18 - 25, 2014 Mostaganem-ALGERIA Second International “Training Courses on Marine Molecular Taxonomy” Mostaganem-ALGERIA, October 18 - 25, 2014 An Overview of the TCMMT Program Prepared by Abdelouahab CHOUIKHI Inter-Islamic Science and Technology Network on Oceanography, Izmir - TURKEY & Karim MEZALI University of Abdelhamid Ibn Badis Mostaganem - Algeria Sponsors: Islamic Development Bank, Jeddah-SAUDI ARABIA Direction Générale de la Recherche Scientifique et du Développement Technologique - MESRS, Algiers-ALGERIA. INOC - University of Mostaganem September 2014 2 Second International Training Courses on Marine Molecular Taxonomy” October 18 - 25, 2014 Mostaganem-ALGERIA Marine Molecular Taxonomy” Second International October 18 - 25, 2014 Training Courses on Mostaganem-ALGERIA 2014 Table of Contents Title Page Venue of the training courses 4 Where to stay? Introduction 5 Objective of the training courses 5 Timetable 7 Syllabus of the lectures 13 LECTURERS 19 Organizational
    [Show full text]
  • 交战和危险地带一览表– 2016 年1 月12 日list of War and Risk Zones
    交战和危险地带一览表 – 2016 年 1 月 12 日 List of War and Risk Zones - Last update: January. 12, 2016 A. 交战地带: A. War zones: 阿富汗:全国 Afghanistan: whole country 阿尔及利亚:塔曼拉塞特省萨赫勒地区。 Algeria: Sahel region in Tamanrasset province 阿塞拜疆:纳戈尔诺-卡拉巴赫共和国 Azerbaijan: Nagorno-Karabakh Republic 布基纳法索:以北地区但不包括加桑,布塞,塞巴。 Burkina Faso: area north of but not including cities of Gassan, Boussé and Sebba 布隆迪:全国地区 Burundi: Whole country 喀麦隆:极北省,喀麦隆北省尼日利亚阿达马瓦州边境 40km 以 Cameroon: Far North province, within 40km of the border with 内,巴卡西半岛,在与乍得边境 40km,并在与中非共和国边境 Nigeria’s Adamawa state in Cameroon’s North province, the Bakassi Peninsula, within 40km of the border with Chad, and within 40km of 40km。 the border with the Central African Republic 中非共和国:全国 Central African Republic: whole country 乍得共和国:与中非共和国陆地边界 30 公里内。远北地区的喀麦 Chad: 30 km from land border with Central African Republic, 30 km 隆省边界 30 公里内。与尼日尔、提贝斯提、博尔库、恩内迪、瓦 from land border with Cameroonian province of Far North, 100 km from land border with Niger, Tibesti, Borkou, Ennedi, Wadi Fira, 迪菲拉、瓦达伊和西拉地区陆地边境 100 公里内。 Ouaddaï and Sila regions 吉布提:去厄立特里亚边境。 Djibouti: to the border with Eritrea 刚果民主共和国(刚果金):南基伍省,北基伍省,东方省的东 Democratic Republic of the Congo: North- and South Kivu state, 部,马尼埃马省,加丹加省,和与中非共和国边境 70km。 eastern Orientale state, Maniema state, Katanga state, and 70 km from border with Central African Republic 埃及:西奈半岛(在沙姆沙伊赫的外围屏障,包括机场和沙姆玛 Egypt:Sinai peninsula (with the exception of the area within the 雅,hadaba,纳马湾,鲨鱼海湾地区纳布克地区除外) Sharm el Sheikh perimeter barrier, which includes the airport and the areas of Sharm el Maya, Hadaba, Naama Bay, Sharks Bay and Nabq) 厄立特里亚:在厄立特里亚与吉布提和埃塞俄比亚边境 25 公里。 Eritrea: within 25km of Eritrea’s border with Djibouti and Ethiopia 埃塞俄比亚:与苏丹和南苏丹边境地区。厄立特里亚边境区域,包 Ethiopia: Border areas with Sudan and South Sudan.
    [Show full text]
  • L'espace Ksourien Monographie Du Ksar Echeikh, Ouled Said, (Timimoun)
    République Algérienne Démocratique et populaire Ministère de l’enseignement supérieur et de recherche scientifique Université Saad Dahlab de Blida 1 Institut d’Architecture et d’Urbanisme Mémoire de fin d’étude en vue de l’obtention du diplôme de Master II en Architecture Option : Architecture et Habitat Thème : L’Espace Ksourien Monographie du Ksar Echeikh, Ouled Said, (Timimoun) Présenté par : Mlle. Sihem ZEBOUDJI Encadré par : Dr. Arch. SAIDI MOHAMED Promotion 2017 - 2018 REMERCIEMENTS Mes vifs remerciements à Monsieur SAIDI pour son encadrement et ses orientations. Que le Dr. Arch. AIT SAADI notre Porteur de Master trouve ici toute ma reconnaissance pour son aide constante et ses conseils précieux. Je tiens à remercier mes amis de Timimoun et de Ouled Said pour leur accueil et leur hospitalité. Je rends hommage à leur grande gentillesse. Une grande reconnaissance à l’Administration du Département d’Architecture de l’université Saad Dahleb,Blida et en l’occurrence le personnel de la bibliothèque. 2 SOMMAIRE REMERCIEMENTS SOMMAIRE RESUME CHAPITRE I : CHAPITRE INTRODUCTIF Introduction I- Présentation de la ville de Ouled Said ………………………….10 II- Problématique spécifique ………………………………………. 11 III- Démarche méthodologique …………………………………..….12 IV- Structure du mémoire ………………………………………..…. 15 CHAPITRE II : ETAT DE LA CONNAISSANCE Introduction II-1- Notions et définitions La crise de la ville……………………………………………….………..17 Le débat sur la ville en Algérie…………………………………………...18 Bilan des instruments d’aménagement et d’urbanisme en Algérie……….18 Critique
    [Show full text]