Synthesis of Three Advanced Biofuels from Ionic Liquid-Pretreated Switchgrass Using Engineered Escherichia Coli
Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli Gregory Bokinskya,b, Pamela P. Peralta-Yahyaa,b, Anthe Georgea,c, Bradley M. Holmesa,c, Eric J. Steena,d, Jeffrey Dietricha,d, Taek Soon Leea,e, Danielle Tullman-Erceka,f, Christopher A. Voigtg, Blake A. Simmonsa,c, and Jay D. Keaslinga,b,d,e,f,1 aJoint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, CA 94608; bQB3 Institute, University of California, San Francisco, CA 94158; cSandia National Laboratories, P.O. Box 969, Livermore, CA 94551; dDepartment of Bioengineering, and ePhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720; fDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720; and gDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 Edited by Alexis T. Bell, University of California, Berkeley, CA, and approved November 2, 2011 (received for review May 2, 2011) One approach to reducing the costs of advanced biofuel production Unfortunately, several challenges must be overcome before from cellulosic biomass is to engineer a single microorganism to lignocellulose can be considered an economically competitive both digest plant biomass and produce hydrocarbons that have feedstock for biofuel production. One of the more significant chal- the properties of petrochemical fuels. Such an organism would lenges is the need for large quantities of glycoside hydrolase (GH) require pathways for hydrocarbon
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