Of the 16 Strains Obtained from Salty Soil in Mongolia, Six Strains Were
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Actinobacterial Diversity of the Ethiopian Rift Valley Lakes
ACTINOBACTERIAL DIVERSITY OF THE ETHIOPIAN RIFT VALLEY LAKES By Gerda Du Plessis Submitted in partial fulfillment of the requirements for the degree of Magister Scientiae (M.Sc.) in the Department of Biotechnology, University of the Western Cape Supervisor: Prof. D.A. Cowan Co-Supervisor: Dr. I.M. Tuffin November 2011 DECLARATION I declare that „The Actinobacterial diversity of the Ethiopian Rift Valley Lakes is my own work, that it has not been submitted for any degree or examination in any other university, and that all the sources I have used or quoted have been indicated and acknowledged by complete references. ------------------------------------------------- Gerda Du Plessis ii ABSTRACT The class Actinobacteria consists of a heterogeneous group of filamentous, Gram-positive bacteria that colonise most terrestrial and aquatic environments. The industrial and biotechnological importance of the secondary metabolites produced by members of this class has propelled it into the forefront of metagenomic studies. The Ethiopian Rift Valley lakes are characterized by several physical extremes, making it a polyextremophilic environment and a possible untapped source of novel actinobacterial species. The aims of the current study were to identify and compare the eubacterial diversity between three geographically divided soda lakes within the ERV focusing on the actinobacterial subpopulation. This was done by means of a culture-dependent (classical culturing) and culture-independent (DGGE and ARDRA) approach. The results indicate that the eubacterial 16S rRNA gene libraries were similar in composition with a predominance of α-Proteobacteria and Firmicutes in all three lakes. Conversely, the actinobacterial 16S rRNA gene libraries were significantly different and could be used to distinguish between sites. -
Actinobacteria in Algerian Saharan Soil and Description Sixteen New
Revue ElWahat pour les Recherches et les Etudes Vol.7n°2 (2014) : 61 – 78 Revue ElWahat pour les recherches et les Etudes : 61 – 78 ISSN : 1112 -7163 Vol.7n°2 (2014) http://elwahat.univ-ghardaia.dz Diversity of Actinobacteria in Algerian Saharan soil and description of sixteen new taxa Bouras Noureddine 1,2 , Meklat Atika 1, Boubetra Dalila 1, Saker Rafika 1, Boudjelal Farida 1, Aouiche Adel 1, Lamari Lynda 1, Zitouni Abdelghani 1, Schumann Peter 3, Spröer Cathrin 3, Klenk Hans -Peter 3 and Sabaou Nasserdine 1 1- Affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria; [email protected] 2- Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, Ghardaïa 47000, Algeria; 3- Leibniz Institute DSMZ - Germ an Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany. Abstract _ The goal of this study was to investigate the biodiversity of actinobacteria in Algerian Saharan soils by using a polyphasic taxonomic approach based on the phenotypic and molecular studies (16S rRNA gene sequence analysis and DNA-DNA hybridization ). A total of 323 strains of actinobacteria were isolated from different soil samples, by a dilution agar plating method, using selective isolation media without or with 15-20% of NaCl (for halophilic strains). The morphological and chemotaxonomic characteristics (diaminopimelic acid isomers, whole-cell sugars, menaquinones, cellular fatty acids and diagnostic phospholipids) of the strains were consistent with those of members of the genus Saccharothrix , Nocardiopsis , Actinopolyspora , Streptomonospora , Saccharopolyspora , Actinoalloteichus , Actinokineospora and Prauserella . -
Genomic and Phylogenomic Insights Into the Family Streptomycetaceae
bioRxiv preprint doi: https://doi.org/10.1101/2020.07.08.193797; this version posted July 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Genomic and phylogenomic insights into the family Streptomycetaceae 2 lead to proposal of Charcoactinosporaceae fam. nov. and 8 novel genera 3 with emended descriptions of Streptomyces calvus 4 Munusamy Madhaiyan1, †, * Venkatakrishnan Sivaraj Saravanan2, † Wah-Seng See-Too3, † 5 1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 6 Singapore 117604; 2Department of Microbiology, Indira Gandhi College of Arts and Science, 7 Kathirkamam 605009, Pondicherry, India; 3Division of Genetics and Molecular Biology, 8 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 9 Malaysia 10 *Corresponding author: Temasek Life Sciences Laboratory, 1 Research Link, National 11 University of Singapore, Singapore 117604; E-mail: [email protected] 12 †All these authors have contributed equally to this work 13 Abstract 14 Streptomycetaceae is one of the oldest families within phylum Actinobacteria and it is large and 15 diverse in terms of number of described taxa. The members of the family are known for their 16 ability to produce medically important secondary metabolites and antibiotics. In this study, 17 strains showing low 16S rRNA gene similarity (<97.3 %) with other members of 18 Streptomycetaceae were identified and subjected to phylogenomic analysis using 33 orthologous 19 gene clusters (OGC) for accurate taxonomic reassignment resulted in identification of eight 20 distinct and deeply branching clades, further average amino acid identity (AAI) analysis showed 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.08.193797; this version posted July 8, 2020. -
Molecular Characterization of Nocardiopsis Species from Didwana Dry Salt Lake of Rajasthan, India
Published online: March 15, 2021 ISSN : 0974-9411 (Print), 2231-5209 (Online) journals.ansfoundation.org Research Article Molecular characterization of Nocardiopsis species from Didwana dry salt lake of Rajasthan, India Khushbu Parihar Mycology and Microbiology Laboratory, Department of Botany, JNV University, Jodhpur-342001 Article Info (Rajasthan), India https://doi.org/10.31018/ Alkesh Tak jans.v13i1.2574 Mycology and Microbiology Laboratory, Department of Botany, JNV University, Jodhpur-342001 Received: February 10, 2021 (Rajasthan), India Revised: March 9, 2021 Praveen Gehlot* Accepted: March 13, 2021 Mycology and Microbiology Laboratory, Department of Botany, JNV University, Jodhpur-342001 (Rajasthan), India Rakesh Pathak ICAR-Central Arid Zone Research Institute, Jodhpur- 342003 (Rajasthan), India Sunil Kumar Singh ICAR-Central Arid Zone Research Institute, Jodhpur- 342003 (Rajasthan), India *Corresponding author. Email: [email protected] How to Cite Parihar, K. et al. (2021). Molecular characterization of Nocardiopsis species from Didwana dry salt lake of Rajasthan, India. Journal of Applied and Natural Science, 13(1): 396 - 401. https://doi.org/10.31018/jans.v13i1.2574 Abstract The genus Nocardiopsis is well known to produce secondary metabolites especially antibacterial bioactive compound. Isolation and characterization of bioactive compounds producing novel isolates from unusual habitats are crucial. The present study was aimed to explore Didwana dry salt lake of Rajasthan state in India for the isolation and characterization of actinomycetes. The isolated actinomycetes isolates were characterized based on culture characteristics, biochemical tests and 16S rRNA gene sequencing. The 16S rRNA gene sequence analysis revealed that all the five isolates inhabiting soil of the said dry salt lake of Didwana, Rajasthan belonged to four species of Nocardiopsis viz., N. -
Successful Drug Discovery Informed by Actinobacterial Systematics
Successful Drug Discovery Informed by Actinobacterial Systematics Verrucosispora HPLC-DAD analysis of culture filtrate Structures of Abyssomicins Biological activity T DAD1, 7.382 (196 mAU,Up2) of 002-0101.D V. maris AB-18-032 mAU CH3 CH3 T extract H3C H3C Antibacterial activity (MIC): S. leeuwenhoekii C34 maris AB-18-032 175 mAU DAD1 A, Sig=210,10 150 C DAD1 B, Sig=230,10 O O DAD1 C, Sig=260,20 125 7 7 500 Rt 7.4 min DAD1 D, Sig=280,20 O O O O Growth inhibition of Gram-positive bacteria DAD1 , Sig=310,20 100 Abyssomicins DAD1 F, Sig=360,40 C 75 DAD1 G, Sig=435,40 Staphylococcus aureus (MRSA) 4 µg/ml DAD1 H, Sig=500,40 50 400 O O 25 O O Staphylococcus aureus (iVRSA) 13 µg/ml 0 CH CH3 300 400 500 nm 3 DAD1, 7.446 (300 mAU,Dn1) of 002-0101.D 300 mAU Mode of action: C HO atrop-C HO 250 atrop-C CH3 CH3 CH3 CH3 200 H C H C H C inhibitior of pABA biosynthesis 200 Rt 7.5 min H3C 3 3 3 Proximicin A Proximicin 150 HO O HO O O O O O O O O O A 100 O covalent binding to Cys263 of PabB 100 N 50 O O HO O O Sea of Japan B O O N O O (4-amino-4-deoxychorismate synthase) by 0 CH CH3 CH3 CH3 3 300 400 500 nm HO HO HO HO Michael addition -289 m 0 B D G H 2 4 6 8 10 12 14 16 min Newcastle Michael Goodfellow, School of Biology, University Newcastle University, Newcastle upon Tyne Atacama Desert In This Talk I will Consider: • Actinobacteria as a key group in the search for new therapeutic drugs. -
Corynebacterium Sp.|NML98-0116
1 Limnochorda_pilosa~GCF_001544015.1@NZ_AP014924=Bacteria-Firmicutes-Limnochordia-Limnochordales-Limnochordaceae-Limnochorda-Limnochorda_pilosa 0,9635 Ammonifex_degensii|KC4~GCF_000024605.1@NC_013385=Bacteria-Firmicutes-Clostridia-Thermoanaerobacterales-Thermoanaerobacteraceae-Ammonifex-Ammonifex_degensii 0,985 Symbiobacterium_thermophilum|IAM14863~GCF_000009905.1@NC_006177=Bacteria-Firmicutes-Clostridia-Clostridiales-Symbiobacteriaceae-Symbiobacterium-Symbiobacterium_thermophilum Varibaculum_timonense~GCF_900169515.1@NZ_LT827020=Bacteria-Actinobacteria-Actinobacteria-Actinomycetales-Actinomycetaceae-Varibaculum-Varibaculum_timonense 1 Rubrobacter_aplysinae~GCF_001029505.1@NZ_LEKH01000003=Bacteria-Actinobacteria-Rubrobacteria-Rubrobacterales-Rubrobacteraceae-Rubrobacter-Rubrobacter_aplysinae 0,975 Rubrobacter_xylanophilus|DSM9941~GCF_000014185.1@NC_008148=Bacteria-Actinobacteria-Rubrobacteria-Rubrobacterales-Rubrobacteraceae-Rubrobacter-Rubrobacter_xylanophilus 1 Rubrobacter_radiotolerans~GCF_000661895.1@NZ_CP007514=Bacteria-Actinobacteria-Rubrobacteria-Rubrobacterales-Rubrobacteraceae-Rubrobacter-Rubrobacter_radiotolerans Actinobacteria_bacterium_rbg_16_64_13~GCA_001768675.1@MELN01000053=Bacteria-Actinobacteria-unknown_class-unknown_order-unknown_family-unknown_genus-Actinobacteria_bacterium_rbg_16_64_13 1 Actinobacteria_bacterium_13_2_20cm_68_14~GCA_001914705.1@MNDB01000040=Bacteria-Actinobacteria-unknown_class-unknown_order-unknown_family-unknown_genus-Actinobacteria_bacterium_13_2_20cm_68_14 1 0,9803 Thermoleophilum_album~GCF_900108055.1@NZ_FNWJ01000001=Bacteria-Actinobacteria-Thermoleophilia-Thermoleophilales-Thermoleophilaceae-Thermoleophilum-Thermoleophilum_album -
Study of Dental Fluorosis in Subjects Related to a Phosphatic Fertilizer
Indian Journal of Geo Marine Sciences Vol. 46 (06), June 2017, pp. 1116-1127 Diversity and enzymatic profile of bacterial flora in the gut of an estuarine fish, Mugil jerdoni Ankita A. Datta, Amit K. Sharma, Rahul Kundu & Satya P. Singh* UGC-CAS Department of Biosciences, Saurashtra University, Rajkot 360 005, India *[E-mail: [email protected]] Received 12 August 2015 ; revised 07 December 2015 In order to examine the bacterial diversity and enzymatic potential, the isolates were screened for the amylolytic, cellulolytic, lipolytic and proteolytic activities using selective media. Significant proportion of the isolates (44%) exhibited lipase activity, while only few (11%) had protease activity. The 16S rRNA gene sequence analysis revealed that most of the isolates related to the genera Bacillus, Acinetobacter, Staphylococcus, Aeromonas, Psychrobacter, Dietzia and Isoptericola. Examined bacteria displayed significant tolerance against varying salt concentrations. Most of the isolates displayed antagonism against 10 selected target organisms. Bacteria were also assessed for their resistance and sensitivity against different antibiotics. Staphylococcus epidermis MJMG8.1 and Dietzia sp. MJMG8.2 grew significantly in the presence of different organic solvents. [Key Words: Microbial enzymes, antibiotic resistance, 16S rRNA sequencing, phylogeny, microbial diversity, fish-gut microflora] Introduction The culturable bacterial flora in the gut of Staphylococcus, unidentified anaerobes and yeast are freshwater and marine water fishes has been explored reported to produce exogenous enzymes. Amylases, in limited sense 1. Bacterial population in the fish gut proteases, lipases, cellulases, chitinases and few is governed by various environmental and intrinsic others have been produced by the gut flora 5, 7. factors such as species, developmental stage, feeding Studies have revealed that the gut microflora prevents strategy, structure of the digestive system and other the establishment of opportunistic pathogens in the physiological factors 2. -
Selective Isolation, Characterisation and Identification of Streptosporangia
SELECTIVE ISOLATION, CHARACTERISATION AND IDENTIFICATION OF STREPTOSPORANGIA Thesissubmitted in accordancewith the requirementsof theUniversity of Newcastleupon Tyne for the Degreeof Doctor of Philosophy by Hong-Joong Kim B. Sc. NEWCASTLE UNIVERSITY LIBRARY ____________________________ 093 51117 X ------------------------------- fn L:L, Iýý:, - L. 51-ý CJ - Departmentof Microbiology, The Medical School,University of Newcastleupon Tyne December1993 CONTENTS ACKNOWLEDGEMENTS Page Number PUBLICATIONS SUMMARY INTRODUCTION A. AIMS 1 B. AN HISTORICAL SURVEY OF THE GENUS STREPTOSPORANGIUM 5 C. NUMERICAL SYSTEMATICS 17 D. MOLECULAR SYSTEMATICS 35 E. CHARACTERISATION OF STREPTOSPORANGIA 41 F. SELECTIVE ISOLATION OF STREPTOSPORANGIA 62 MATERIALS AND METHODS A. SELECTIVE ISOLATION, ENUMERATION AND 75 CHARACTERISATION OF STREPTOSPORANGIA B. NUMERICAL IDENTIFICATION 85 C. SEQUENCING OF 5S RIBOSOMAL RNA 101 D. PYROLYSIS MASS SPECTROMETRY 103 E. RAPID ENZYME TESTS 113 RESULTS A. SELECTIVE ISOLATION, ENUMERATION AND 122 CHARACTERISATION OF STREPTOSPORANGIA B. NUMERICAL IDENTIFICATION OF STREPTOSPORANGIA 142 C. PYROLYSIS MASS SPECTROMETRY 178 D. 5S RIBOSOMAL RNA SEQUENCING 185 E. RAPID ENZYME TESTS 190 DISCUSSION A. SELECTIVE ISOLATION 197 B. CLASSIFICATION 202 C. IDENTIFICATION 208 D. FUTURE STUDIES 215 REFERENCES 220 APPENDICES A. TAXON PROGRAM 286 B. MEDIA AND REAGENTS 292 C. RAW DATA OF PRACTICAL EVALUATION 295 D. RAW DATA OF IDENTIFICATION 297 E. RAW DATA OF RAPID ENZYME TESTS 300 ACKNOWLEDGEMENTS I would like to sincerely thank my supervisor, Professor Michael Goodfellow for his assistance,guidance and patienceduring the course of this study. I am greatly indebted to Dr. Yong-Ha Park of the Genetic Engineering Research Institute in Daejon, Korea for his encouragement, for giving me the opportunity to extend my taxonomic experience and for carrying out the 5S rRNA sequencing studies. -
Elucidating the Molecular Physiology of Lantibiotic NAI-107 Production in Microbispora ATCC-PTA-5024
Downloaded from orbit.dtu.dk on: Sep 28, 2021 Elucidating the molecular physiology of lantibiotic NAI-107 production in Microbispora ATCC-PTA-5024 Gallo, Giuseppe; Renzone, Giovanni; Palazzotto, Emilia; Monciardini, Paolo; Arena, Simona; Faddetta, Teresa; Giardina, Anna; Alduina, Rosa; Weber, Tilmann; Sangiorgi, Fabio Total number of authors: 15 Published in: B M C Genomics Link to article, DOI: 10.1186/s12864-016-2369-z Publication date: 2016 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Gallo, G., Renzone, G., Palazzotto, E., Monciardini, P., Arena, S., Faddetta, T., Giardina, A., Alduina, R., Weber, T., Sangiorgi, F., Russo, A., Spinelli, G., Sosio, M., Scaloni, A., & Puglia, A. M. (2016). Elucidating the molecular physiology of lantibiotic NAI-107 production in Microbispora ATCC-PTA-5024. B M C Genomics, 17(42). https://doi.org/10.1186/s12864-016-2369-z General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. -
Nocardiopsis Algeriensis Sp. Nov., an Alkalitolerant Actinomycete Isolated from Saharan Soil
Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil Noureddine Bouras, Atika Meklat, Abdelghani Zitouni, Florence Mathieu, Peter Schumann, Cathrin Spröer, Nasserdine Sabaou, Hans-Peter Klenk To cite this version: Noureddine Bouras, Atika Meklat, Abdelghani Zitouni, Florence Mathieu, Peter Schumann, et al.. Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil. Antonie van Leeuwenhoek, Springer Verlag, 2015, 107 (2), pp.313-320. 10.1007/s10482-014-0329-7. hal- 01894564 HAL Id: hal-01894564 https://hal.archives-ouvertes.fr/hal-01894564 Submitted on 12 Oct 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. 2SHQ$UFKLYH7RXORXVH$UFKLYH2XYHUWH 2$7$2 2$7$2 LV DQ RSHQ DFFHVV UHSRVLWRU\ WKDW FROOHFWV WKH ZRUN RI VRPH 7RXORXVH UHVHDUFKHUVDQGPDNHVLWIUHHO\DYDLODEOHRYHUWKHZHEZKHUHSRVVLEOH 7KLVLVan author's YHUVLRQSXEOLVKHGLQhttp://oatao.univ-toulouse.fr/20349 2IILFLDO85/ http://doi.org/10.1007/s10482-014-0329-7 7RFLWHWKLVYHUVLRQ Bouras, Noureddine and Meklat, Atika and Zitouni, Abdelghani and Mathieu, Florence and Schumann, Peter and Spröer, Cathrin and Sabaou, Nasserdine and Klenk, Hans-Peter Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil. (2015) Antonie van Leeuwenhoek, 107 (2). 313-320. ISSN 0003-6072 $Q\FRUUHVSRQGHQFHFRQFHUQLQJWKLVVHUYLFHVKRXOGEHVHQWWRWKHUHSRVLWRU\DGPLQLVWUDWRU WHFKRDWDR#OLVWHVGLIILQSWRXORXVHIU Nocardiopsis algeriensis sp. -
Report on 31 Unrecorded Bacterial Species in Korea That Belong to the Phylum Actinobacteria
Journal of Species Research 5(1):113, 2016 Report on 31 unrecorded bacterial species in Korea that belong to the phylum Actinobacteria JungHye Choi1, JuHee Cha1, JinWoo Bae2, JangCheon Cho3, Jongsik Chun4, WanTaek Im5, Kwang Yeop Jahng6, Che Ok Jeon7, Kiseong Joh8, Seung Bum Kim9, Chi Nam Seong10, JungHoon Yoon11 and ChangJun Cha1,* 1Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Korea 2Department of Biology, Kyung Hee University, Seoul 02447, Korea 3Department of Biological Sciences, Inha University, Incheon 22212, Korea 4School of Biological Sciences, Seoul National University, Seoul 08826, Korea 5Department of Biotechnology, Hankyong National University, Anseong 17579, Korea 6Department of Life Sciences, Chonbuk National University, Jeonju-si 54896, Korea 7Department of Life Science, Chung-Ang University, Seoul 06974, Korea 8Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, Korea 9Department of Microbiology, Chungnam National University, Daejeon 34134, Korea 10Department of Biology, Sunchon National University, Suncheon 57922, Korea 11Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 16419, Korea *Correspondent: [email protected] To discover and characterize indigenous species in Korea, a total of 31 bacterial strains that belong to the phylum Actinobacteria were isolated from various niches in Korea. Each strain showed the high sequence similarity (>99.1%) with the closest bacterial species, forming a robust phylogenetic clade. These strains have not been previously recorded in Korea. According to the recently updated taxonomy of the phylum Actinobacteria based upon 16S rRNA trees, we report 25 genera of 13 families within 5 orders of the class Actinobacteria as actinobacterial species found in Korea. -
Exploiting the Aerobic Endospore-Forming Bacterial
Couto et al. BMC Microbiology (2015) 15:240 DOI 10.1186/s12866-015-0575-5 RESEARCH ARTICLE Open Access Exploiting the aerobic endospore-forming bacterial diversity in saline and hypersaline environments for biosurfactant production Camila Rattes de Almeida Couto, Vanessa Marques Alvarez, Joana Montezano Marques, Diogo de Azevedo Jurelevicius and Lucy Seldin* Abstract Background: Biosurfactants are surface-active biomolecules with great applicability in the food, pharmaceutical and oil industries. Endospore-forming bacteria, which survive for long periods in harsh environments, are described as biosurfactant producers. Although the ubiquity of endospore-forming bacteria in saline and hypersaline environments is well known, studies on the diversity of the endospore-forming and biosurfactant-producing bacterial genera/species in these habitats are underrepresented. Methods: In this study, the structure of endospore-forming bacterial communities in sediment/mud samples from Vermelha Lagoon, Massambaba, Dois Rios and Abraão Beaches (saline environments), as well as the Praia Seca salterns (hypersaline environments) was determined via denaturing gradient gel electrophoresis. Bacterial strains were isolated from these environmental samples and further identified using 16S rRNA gene sequencing. Strains presenting emulsification values higher than 30 % were grouped via BOX-PCR, and the culture supernatants of representative strains were subjected to high temperatures and to the presence of up to 20 % NaCl to test their emulsifying activities in these extreme conditions. Mass spectrometry analysis was used to demonstrate the presence of surfactin. Results: A diverse endospore-forming bacterial community was observed in all environments. The 110 bacterial strains isolated from these environmental samples were molecularly identified as belonging to the genera Bacillus, Thalassobacillus, Halobacillus, Paenibacillus, Fictibacillus and Paenisporosarcina.