Supporting Information for: ZmCCT9 enhances maize adaptation to higher latitudes Cheng Huanga, Huayue Suna, Dingyi Xua, Qiuyue Chena, Yameng Lianga, Xufeng Wanga, Guanghui Xua, Jinge Tiana, Chenglong Wanga, Dan Lia, Lishuan Wua, Xiaohong Yanga, Weiwei Jina, John F. Doebleyb,1, and Feng Tiana,1 aNational Maize Improvement Center of China, Beijing Key Laboratory of Crop Genetic Improvement, Laboratory of Crop Heterosis and Utilization, Joint International Research Laboratory of Crop Molecular Breeding, China Agricultural University, Beijing 100193, China; and bDepartment of Genetics, University of Wisconsin, Madison, WI 53706 1To whom correspondence may be addressed. Email:
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[email protected]. SI Materials and Methods Plant Materials. The population of 866 maize-teosinte BC2S3 RILs was obtained from the Maize Genetics Cooperation Stock Center (Maize COOP). This population was derived from a cross between W22, a typical temperate maize (Zea mays ssp. mays) inbred line, as the recurrent parent, and CIMMYT accession 8759, a typical accession of teosinte (Zea mays ssp. parviglumis), as the donor parent. Detailed information on this population has been previously reported (1-4). A maize association panel composed of 513 diverse maize inbred lines (5) was used in the association analysis of the 2.4-kb causative region for qDTA9. To analyse the evolutionary origin of the Harbinger-like element, a panel of 73 diverse teosinte lines (Zea mays ssp. parviglumis) (SI Appendix, Table S1), representing the genetic and geographic variability within extant populations of the progenitor species, was genotyped using primers specific to the Harbinger-like element. Another diverse panel, including 27 maize inbred lines and 19 teosinte accessions (Zea mays ssp.