中国科技论文在线 Pusillimonas Oleiphila Sp. Nov., a New Member Of
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Metaproteogenomic Insights Beyond Bacterial Response to Naphthalene
ORIGINAL ARTICLE ISME Journal – Original article Metaproteogenomic insights beyond bacterial response to 5 naphthalene exposure and bio-stimulation María-Eugenia Guazzaroni, Florian-Alexander Herbst, Iván Lores, Javier Tamames, Ana Isabel Peláez, Nieves López-Cortés, María Alcaide, Mercedes V. del Pozo, José María Vieites, Martin von Bergen, José Luis R. Gallego, Rafael Bargiela, Arantxa López-López, Dietmar H. Pieper, Ramón Rosselló-Móra, Jesús Sánchez, Jana Seifert and Manuel Ferrer 10 Supporting Online Material includes Text (Supporting Materials and Methods) Tables S1 to S9 Figures S1 to S7 1 SUPPORTING TEXT Supporting Materials and Methods Soil characterisation Soil pH was measured in a suspension of soil and water (1:2.5) with a glass electrode, and 5 electrical conductivity was measured in the same extract (diluted 1:5). Primary soil characteristics were determined using standard techniques, such as dichromate oxidation (organic matter content), the Kjeldahl method (nitrogen content), the Olsen method (phosphorus content) and a Bernard calcimeter (carbonate content). The Bouyoucos Densimetry method was used to establish textural data. Exchangeable cations (Ca, Mg, K and 10 Na) extracted with 1 M NH 4Cl and exchangeable aluminium extracted with 1 M KCl were determined using atomic absorption/emission spectrophotometry with an AA200 PerkinElmer analyser. The effective cation exchange capacity (ECEC) was calculated as the sum of the values of the last two measurements (sum of the exchangeable cations and the exchangeable Al). Analyses were performed immediately after sampling. 15 Hydrocarbon analysis Extraction (5 g of sample N and Nbs) was performed with dichloromethane:acetone (1:1) using a Soxtherm extraction apparatus (Gerhardt GmbH & Co. -
Potential for and Distribution of Enzymatic Biodegradation of Polystyrene by Environmental Microorganisms
materials Communication Potential for and Distribution of Enzymatic Biodegradation of Polystyrene by Environmental Microorganisms Liyuan Hou and Erica L.-W. Majumder * Department of Chemistry, SUNY College of Environmental Science and Forestry, Syracuse, NY 13210, USA; [email protected] * Correspondence: [email protected] or [email protected]; Tel.: +1-3154706854 Abstract: Polystyrene (PS) is one of the main polymer types of plastic wastes and is known to be resistant to biodegradation, resulting in PS waste persistence in the environment. Although previous studies have reported that some microorganisms can degrade PS, enzymes and mechanisms of microorganism PS biodegradation are still unknown. In this study, we summarized microbial species that have been identified to degrade PS. By screening the available genome information of microorganisms that have been reported to degrade PS for enzymes with functional potential to depolymerize PS, we predicted target PS-degrading enzymes. We found that cytochrome P4500s, alkane hydroxylases and monooxygenases ranked as the top potential enzyme classes that can degrade PS since they can break C–C bonds. Ring-hydroxylating dioxygenases may be able to break the side-chain of PS and oxidize the aromatic ring compounds generated from the decomposition of PS. These target enzymes were distributed in Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes, suggesting a broad potential for PS biodegradation in various earth environments and microbiomes. Our results provide insight into the enzymatic degradation of PS and suggestions for realizing the biodegradation of this recalcitrant plastic. Citation: Hou, L.; Majumder, E.L. Keywords: plastics; polystyrene biodegradation; enzymatic biodegradation; monooxygenase; alkane Potential for and Distribution of hydroxylase; cytochrome P450 Enzymatic Biodegradation of Polystyrene by Environmental Microorganisms. -
Taxonomic Hierarchy of the Phylum Proteobacteria and Korean Indigenous Novel Proteobacteria Species
Journal of Species Research 8(2):197-214, 2019 Taxonomic hierarchy of the phylum Proteobacteria and Korean indigenous novel Proteobacteria species Chi Nam Seong1,*, Mi Sun Kim1, Joo Won Kang1 and Hee-Moon Park2 1Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea 2Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea *Correspondent: [email protected] The taxonomic hierarchy of the phylum Proteobacteria was assessed, after which the isolation and classification state of Proteobacteria species with valid names for Korean indigenous isolates were studied. The hierarchical taxonomic system of the phylum Proteobacteria began in 1809 when the genus Polyangium was first reported and has been generally adopted from 2001 based on the road map of Bergey’s Manual of Systematic Bacteriology. Until February 2018, the phylum Proteobacteria consisted of eight classes, 44 orders, 120 families, and more than 1,000 genera. Proteobacteria species isolated from various environments in Korea have been reported since 1999, and 644 species have been approved as of February 2018. In this study, all novel Proteobacteria species from Korean environments were affiliated with four classes, 25 orders, 65 families, and 261 genera. A total of 304 species belonged to the class Alphaproteobacteria, 257 species to the class Gammaproteobacteria, 82 species to the class Betaproteobacteria, and one species to the class Epsilonproteobacteria. The predominant orders were Rhodobacterales, Sphingomonadales, Burkholderiales, Lysobacterales and Alteromonadales. The most diverse and greatest number of novel Proteobacteria species were isolated from marine environments. Proteobacteria species were isolated from the whole territory of Korea, with especially large numbers from the regions of Chungnam/Daejeon, Gyeonggi/Seoul/Incheon, and Jeonnam/Gwangju. -
Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater
sustainability Article Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater Tamara Nazina 1,2,* , Tamara Babich 1, Nadezhda Kostryukova 1, Diyana Sokolova 1, Ruslan Abdullin 1, Tatyana Tourova 1, Vitaly Kadnikov 3, Andrey Mardanov 3, Nikolai Ravin 3, Denis Grouzdev 3 , Andrey Poltaraus 4, Stepan Kalmykov 5, Alexey Safonov 6, Elena Zakharova 6, Alexander Novikov 2 and Kenji Kato 7 1 Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia; [email protected] (T.B.); [email protected] (N.K.); [email protected] (D.S.); [email protected] (R.A.); [email protected] (T.T.) 2 V.I. Vernadsky Institute of Geochemistry and Analytical Chemistry of Russian Academy of Sciences, 119071 Moscow, Russia; [email protected] 3 Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia; [email protected] (V.K.); [email protected] (A.M.); [email protected] (N.R.); [email protected] (D.G.) 4 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119071 Moscow, Russia; [email protected] 5 Chemical Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; [email protected] 6 Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071 Moscow, Russia; [email protected] (A.S.); [email protected] (E.Z.) 7 Faculty of Science, Department of Geosciences, Shizuoka University, 422-8529 Shizuoka, Japan; [email protected] -
Appendix 1. Validly Published Names, Conserved and Rejected Names, And
Appendix 1. Validly published names, conserved and rejected names, and taxonomic opinions cited in the International Journal of Systematic and Evolutionary Microbiology since publication of Volume 2 of the Second Edition of the Systematics* JEAN P. EUZÉBY New phyla Alteromonadales Bowman and McMeekin 2005, 2235VP – Valid publication: Validation List no. 106 – Effective publication: Names above the rank of class are not covered by the Rules of Bowman and McMeekin (2005) the Bacteriological Code (1990 Revision), and the names of phyla are not to be regarded as having been validly published. These Anaerolineales Yamada et al. 2006, 1338VP names are listed for completeness. Bdellovibrionales Garrity et al. 2006, 1VP – Valid publication: Lentisphaerae Cho et al. 2004 – Valid publication: Validation List Validation List no. 107 – Effective publication: Garrity et al. no. 98 – Effective publication: J.C. Cho et al. (2004) (2005xxxvi) Proteobacteria Garrity et al. 2005 – Valid publication: Validation Burkholderiales Garrity et al. 2006, 1VP – Valid publication: Vali- List no. 106 – Effective publication: Garrity et al. (2005i) dation List no. 107 – Effective publication: Garrity et al. (2005xxiii) New classes Caldilineales Yamada et al. 2006, 1339VP VP Alphaproteobacteria Garrity et al. 2006, 1 – Valid publication: Campylobacterales Garrity et al. 2006, 1VP – Valid publication: Validation List no. 107 – Effective publication: Garrity et al. Validation List no. 107 – Effective publication: Garrity et al. (2005xv) (2005xxxixi) VP Anaerolineae Yamada et al. 2006, 1336 Cardiobacteriales Garrity et al. 2005, 2235VP – Valid publica- Betaproteobacteria Garrity et al. 2006, 1VP – Valid publication: tion: Validation List no. 106 – Effective publication: Garrity Validation List no. 107 – Effective publication: Garrity et al. -
A Genomic Perspective on a New Bacterial Genus and Species From
Whiteson et al. BMC Genomics 2014, 15:169 http://www.biomedcentral.com/1471-2164/15/169 RESEARCH ARTICLE Open Access A genomic perspective on a new bacterial genus and species from the Alcaligenaceae family, Basilea psittacipulmonis Katrine L Whiteson1,5*, David Hernandez1, Vladimir Lazarevic1, Nadia Gaia1, Laurent Farinelli2, Patrice François1, Paola Pilo3, Joachim Frey3 and Jacques Schrenzel1,4 Abstract Background: A novel Gram-negative, non-haemolytic, non-motile, rod-shaped bacterium was discovered in the lungs of a dead parakeet (Melopsittacus undulatus) that was kept in captivity in a petshop in Basel, Switzerland. The organism is described with a chemotaxonomic profile and the nearly complete genome sequence obtained through the assembly of short sequence reads. Results: Genome sequence analysis and characterization of respiratory quinones, fatty acids, polar lipids, and biochemical phenotype is presented here. Comparison of gene sequences revealed that the most similar species is Pelistega europaea, with BLAST identities of only 93% to the 16S rDNA gene, 76% identity to the rpoB gene, and a similar GC content (~43%) as the organism isolated from the parakeet, DSM 24701 (40%). The closest full genome sequences are those of Bordetella spp. and Taylorella spp. High-throughput sequencing reads from the Illumina-Solexa platform were assembled with the Edena de novo assembler to form 195 contigs comprising the ~2 Mb genome. Genome annotation with RAST, construction of phylogenetic trees with the 16S rDNA (rrs) gene sequence and the rpoB gene, and phylogenetic placement using other highly conserved marker genes with ML Tree all suggest that the bacterial species belongs to the Alcaligenaceae family. -
Bioremediation of Clay with High Oil Content and Biological Response After Restoration Xiaokang Li1, Jinling Li1*, Chengtun Qu1,2, Tao Yu1 & Mingming Du1
www.nature.com/scientificreports OPEN Bioremediation of clay with high oil content and biological response after restoration Xiaokang Li1, Jinling Li1*, Chengtun Qu1,2, Tao Yu1 & Mingming Du1 The clay with high oil content form soil lumps, which is hard for microbes to repair. In this paper, the bioaugmentation and biostimulation technology were applied to improve the bioremediation efect of the soil with high oil content, that modifed by local cow dung and sandy soil, the ecological toxicity of the soil after restoration was further analyzed. After 53 days of bioremediation, the degradation efciency with respect to the total petroleum hydrocarbons (TPH) content reached 76.9% ± 2.2%. The soil bacterial count of M5 group reached log10 CFU/g soil = 7.69 ± 0.03 and the results were better than other experimental groups. The relative abundances of petroleum-degrading bacteria added to M5 remained high (Achromobacter 9.44%, Pseudomonas 31.06%, and Acinetobacter 14.11%), and the proportions of some other indigenous bacteria (Alcanivorax and Paenibacillus) also increased. The toxicity of the bioremediated soil was reduced by seed germination and earthworm survival experiments. During the mining, transportation, and refning of oil and gas, the leakage of amounts of oil causes lasting envi- ronmental damage. Nearly 3 billion tons of oil is exploited annually worldwide, of which 8 million tons fow into the environment, producing as much as 1 billion tons of contaminated soil and sludge. Petroleum oil bind to soil particles, reducing soil porosity and obstructing the efective absorption of nutrients by petroleum-degrading bacteria. Moreover, petroleum oil can also attach to the surfaces of plant roots and prevents plants from absorb- ing nutrients, leading to the death of various plants and the subsequent worsening of soil pollution 1. -
S1. the Genomes of the Order Burkholderiales (Taxid:80840) in The
S1. The genomes of the order Burkholderiales (taxid:80840) in the NCBI Genome List (http://www.ncbi.nlm.nih.gov/genome/browse/) 05-09-2014 N Organism/Name Kingdom Group SubGroup Size (Mb) Chr Plasmids Assemblies 1. Burkholderiales bacterium JGI 0001003-L21 Bacteria Proteobacteria Betaproteobacteria 0,070281 - - 1 2. Comamonadaceae bacterium JGI 0001003-E14 Bacteria Proteobacteria Betaproteobacteria 0,113075 - - 1 3. Candidatus Zinderia insecticola Bacteria Proteobacteria Betaproteobacteria 0,208564 1 - 1 4. Comamonadaceae bacterium JGI 0001013-A16 Bacteria Proteobacteria Betaproteobacteria 0,295955 - - 1 5. Oxalobacteraceae bacterium JGI 0001004-J12 Bacteria Proteobacteria Betaproteobacteria 0,301938 - - 1 6. Oxalobacteraceae bacterium JGI 0001002-K6 Bacteria Proteobacteria Betaproteobacteria 0,368093 - - 1 7. Burkholderiales bacterium JGI 0001003-J08 Bacteria Proteobacteria Betaproteobacteria 0,394907 - - 1 8. Burkholderiales bacterium JGI 0001002-H06 Bacteria Proteobacteria Betaproteobacteria 0,415398 - - 1 9. Pelomonas Bacteria Proteobacteria Betaproteobacteria 0,507844 - - 2 10. Leptothrix ochracea Bacteria Proteobacteria Betaproteobacteria 0,510792 - - 1 11. Oxalobacteraceae bacterium JGI 0001012-C15 Bacteria Proteobacteria Betaproteobacteria 0,640975 - - 1 12. Burkholderiales bacterium JGI 0001003-A5 Bacteria Proteobacteria Betaproteobacteria 0,686115 - - 1 13. Oxalobacteraceae bacterium JGI 001010-B17 Bacteria Proteobacteria Betaproteobacteria 0,825556 - - 1 14. Rhizobacter Bacteria Proteobacteria Betaproteobacteria 1,05799 - - -
Soil Microbiome Structure and Function in Ecopiles Used to Remediate Petroleum-Contaminated Soil
See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/350063178 Soil Microbiome Structure and Function in Ecopiles Used to Remediate Petroleum-Contaminated Soil Article in Frontiers in Environmental Science · March 2021 DOI: 10.3389/fenvs.2021.624070 CITATIONS READS 0 113 11 authors, including: Mutian Wang Daniel Garrido-Sanz Institute of Technology, Carlow University of Lausanne 2 PUBLICATIONS 8 CITATIONS 26 PUBLICATIONS 237 CITATIONS SEE PROFILE SEE PROFILE Paula San Segundo Miguel Redondo-Nieto Universidad Autónoma de Madrid Universidad Autónoma de Madrid 5 PUBLICATIONS 5 CITATIONS 86 PUBLICATIONS 1,258 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: endophyte inoculants View project Nutrient-recycling-from-pilot-production-to-farms-and-fields - ReNu2Farm View project All content following this page was uploaded by David N Dowling on 15 March 2021. The user has requested enhancement of the downloaded file. ORIGINAL RESEARCH published: 15 March 2021 doi: 10.3389/fenvs.2021.624070 Soil Microbiome Structure and Function in Ecopiles Used to Remediate Petroleum-Contaminated Soil M. Wang 1†, D. Garrido-Sanz 2†, P. Sansegundo-Lobato 2, M. Redondo-Nieto 2, R. Conlon 1, M. Martin 2, R. Mali 3, X. Liu 3, D. N. Dowling 1, R. Rivilla 2 and K. J. Germaine 1* 1EnviroCore, Dargan Research Centre, Institute of Technology Carlow, Carlow, Ireland, 2Departamento de Biología, Facultad de Edited by: Ciencias, Universidad Autónoma de Madrid, Madrid, Spain, 3MicroGen Biotech Ltd., Enterprise, Research and Innovation Centre, Markus Puschenreiter, Green Road, Carlow, Ireland University of Natural Resources and Life Sciences Vienna, Austria The soil microbiome consists of a vast variety of microorganisms which contribute to Reviewed by: Bhabananda Biswas, essential ecosystem services including nutrient recycling, protecting soil structure, and University of South Australia, Australia pathogen suppression. -
Contents of Phd Thesis
博士論文 Isolation and Identification of Novel Species of Heavy-metals Tolerant Bacteria from Pakistan for Their Potential Usage in Bioremediation and in Agriculture (パキスタン由来の重金属耐性を示す新種細菌の 単離•同定及びそのバイオレメディエーションや 農業への応用の可能性についての研究) By SAIRA ABBAS サイラ アバス DEPARTMENT OF APPLIED BIOLOGICAL CHEMISTRY GRADUATE SCHOOL OF AGRICULTURE AND LIFE SCIENCES THE UNIVERSITY OF TOKYO TOKYO-JAPAN 2016 博士論文 Isolation and Identification of Novel Species of Heavy- metals Tolerant Bacteria from Pakistan for Their Potential Usage in Bioremediation and in Agriculture (パキスタン由来の重金属耐性を示す新種細菌の 単離•同定及びそのバイオレメディエーションや 農業への応用の可能性についての研究) By SAIRA ABBAS サイラ アバス DEPARTMENT OF APPLIED BIOLOGICAL CHEMISTRY GRADUATE SCHOOL OF AGRICULTURE AND LIFE SCIENCES THE UNIVERSITY OF TOKYO TOKYO-JAPAN 2016 ( パ of Heavy of Species Novel Identification Isolation and キ ス タ ン 由 来 の 重 金 属 耐 農 性 業 を へ 示 の す 応 新 - 用 種 in from for and Usage Bioremediation Their Potential Pakistan Bacteria Tolerant metals の 細 可 菌 能 の 性 単 に 離 つ • い 同 て 定 の 及 研 び 究 そ ) の バ イ オ レ メ デ ィ エ ー シ ョ ン や サ Agriculture in SAIRA ABBAS イ ラ ア バ ス Isolation and Identification of Novel Species of Heavy- metals Tolerant Bacteria from Pakistan for Their Potential Usage in Bioremediation and in Agriculture (パキスタン由来の重金属耐性を示す新種細菌の 単離•同定及びそのバイオレメディエーションや 農業への応用の可能性についての研究) By SAIRA ABBAS サイラ アバス A thesis submitted as a requirement for the degree of DOCTORATE OF PHILOSOPHY IN ENVIRONMENTAL MICROBIOLOGY AND BIOTECHNOLOGY DEPARTMENT OF APPLIED BIOLOGICAL CHEMISTRY GRADUATE SCHOOL OF AGRICULTURE AND LIFE SCIENCES THE UNIVERSITY OF TOKYO TOKYO-JAPAN 2016 Dedicated to My loving Mother and Father Who always pray To see the bud of Their wishes Bloom into a Flower CERTIFICATE ''It is certified that the contents and form of the thesis entitled “Isolation and Identification of Novel Species of Heavy-metals Tolerant Bacteria from Pakistan for Their Potential Usage in Bioremediation and in Agriculture ” submitted by Ms. -
Castellaniella Gen. Nov., to Accommodate the Phylogenetic Lineage of Alcaligenes Defragrans, and Proposal of Castellaniella Defragrans Gen
International Journal of Systematic and Evolutionary Microbiology (2006), 56, 815–819 DOI 10.1099/ijs.0.63989-0 Castellaniella gen. nov., to accommodate the phylogenetic lineage of Alcaligenes defragrans, and proposal of Castellaniella defragrans gen. nov., comb. nov. and Castellaniella denitrificans sp. nov. P. Ka¨mpfer,1 K. Denger,2 A. M. Cook,2 S.-T. Lee,3 U. Ja¨ckel,1 E. B. M. Denner4 and H.-J. Busse4 Correspondence 1Institut fu¨r Angewandte Mikrobiologie, Justus Liebig Universita¨t, IFZ – Heinrich-Buff-Ring P. Ka¨mpfer 26–32, D-35392 Giessen, Germany [email protected] 2Department of Biology, The University of Konstanz, D-78457 Konstanz, Germany giessen.de 3Department of Biological Sciences, Korean Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejon, 305-701, Republic of Korea 4Institut fu¨r Bakteriologie, Mykologie und Hygiene, Veterina¨rmedizinische Universita¨t Wien, Veterina¨rplatz 1, A-1210 Wien, Austria Comparative 16S rRNA gene sequence analysis indicates that two distinct sublineages exist within the genus Alcaligenes: the Alcaligenes faecalis lineage, comprising Alcaligenes aquatilis and A. faecalis (with the three subspecies A. faecalis subsp. faecalis, A. faecalis subsp. parafaecalis and A. faecalis subsp. phenolicus), and the Alcaligenes defragrans lineage, comprising A. defragrans. This phylogenetic discrimination is supported by phenotypic and chemotaxonomic differences. It is proposed that the A. defragrans lineage constitutes a distinct genus, for which the name Castellaniella gen. nov. is proposed. The type strain for Castellaniella defragrans gen. nov., comb. nov. is 54PinT (=CCUG 39790T=CIP 105602T=DSM 12141T). Finally, on the basis of data from the literature and new DNA–DNA hybridization and phenotypic data, the novel species Castellaniella denitrificans sp. -
1 TITLE: Candidimonas Nitroreducens Sp
1 TITLE: Candidimonas nitroreducens sp. nov. and Candidimonas humi sp. nov. , isolated 2 from sewage sludge compost 3 4 AUTHORS: Ivone Vaz-Moreira1,2, Vânia Figueira1, Ana R. Lopes2, Evie De Brandt 3, Peter 5 Vandamme3, Olga C. Nunes2, Célia M. Manaia1 6 7 1CBQF - Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 8 Porto, Portugal 9 2LEPAE – Departamento de Engenharia Química, Faculdade de Engenharia, Universidade 10 do Porto, 4200-465 Porto, Portugal 11 3 Laboratorium voor Microbiologie, Vakgroep Biochemie en Microbiologie, Universiteit 12 Gent, Gent, Belgium 13 14 15 AUTHOR FOR CORRESPONDENCE: 16 C.M. Manaia, PhD 17 Escola Superior de Biotecnologia 18 Universidade Católica Portuguesa 19 4200-072 Porto 20 Portugal 21 Tel: +351 22 5580059 22 Fax: +351 22 5090351 23 e-mail: [email protected] 24 25 RUNNING TITLE: Candidimonas gen. nov. 26 SUBJECT CATEGORY: NEW TAXA – PROTEOBACTERIA 27 FOOTNOTE: The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene 28 sequence of the isolate SC-089T and SC-092T are FN556191 and FN556192, respectively. 29 1 30 SUMMARY 31 Two bacterial strains isolated from sewage sludge compost (SC-089T and SC-092T) were 32 characterized using a polyphasic approach. The isolates were Gram-negative short rods, 33 catalase- and oxidase-positive, with good growth at 30 ºC, pH 7 and 1 % (w/v) of NaCl. 34 Ubiquinone 8 was the major respiratory quinone and phosphatidylethanolamine, 35 phosphatidylglycerol and diphosphatidylglycerol were amongst the major polar lipids. On 36 basis of the 16S rRNA gene sequence analysis, the strains were observed to be members of 37 the family Alcaligenaceae, but could not be identified as members of any validly named 38 genus.