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(12) United States Patent (10) Patent No.: US 6,911,331 B2 Famodu Et Al

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(12) United States Patent (10) Patent No.: US 6,911,331 B2 Famodu Et Al USOO691.1331 B2 (12) United States Patent (10) Patent No.: US 6,911,331 B2 Famodu et al. (45) Date of Patent: Jun. 28, 2005 (54) CHORISMATE BIOSYNTHESIS ENZYMES William E. Dyer et al., Journ. of Biol Chem., vol. 265 1608–1614, 1990, A cDNA Encoding 3-Deoxy-D-arabino (75) Inventors: Omolayo O. Famodu, Newark, DE heptulosonate 7-Phosphate Synthase from Solanum tubero (US); William D. Hitz, Wilmington, sum L., XP-002129967. DE (US); Jonathan E. Lightner, Jorn Gorlach et al., Plant Mol. Biol., vol. 23:697–706, 1993, Mulino, OR (US) Differential expression of tomato (LycopersicOn eSculentum L.) genes encoding Shikimate pathway isoenzymes. I. (73) Assignee: E. I. du Pont de Nemours and 3-Deoxy-D-arabino-heptuloSonate 7-phosphate Synthase, Company, Wilmington, DE (US) XP-002121067. James D. Jones et al., Plant Physiol., vol. 108(4): 1413-1421, (*) Notice: Subject to any disclaimer, the term of this 1995, Impaired Wound Induction of patent is extended or adjusted under 35 3-Deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) U.S.C. 154(b) by 27 days. Synthase and Altered Stem Development in Transgenic Potato Plants Expressing a DAHP Synthase Antisense Con (21) Appl. No.: 10/105,729 struct, XP-002121068. Klaus M. Herrmann, Plant Physiol., vol. 107:Jul. 12, 1995. (22) Filed: Mar 22, 2002 The shikimate Pathway as an Entry to Aromatic Secondary (65) Prior Publication Data Metabolism, XP-002121069. EMBL Database Accession No.: AI677182, May 25, 1999, US 2003/0003557 A1 Jan. 2, 2003 Walbot, V., Maize ESTs from various cDNA libraries sequenced at Stanford University, XP-002121070. Related U.S. Application Data EMBL Database Accession No.: AI615213, Apr. 26, 1999, Walbot, V., Maize ESTs from various cDNA libraries (63) Continuation-in-part of application No. 09/743,210, filed on sequenced at Stanford University, XP-002129968. Feb. 23, 2001, now abandoned. (60) Provisional application No. PCT/US99/16352, filed on Jul. EMBL Database Accession No.: AI443687, Mar. 16, 1999, 20, 1999, and provisional application No. 60/093.611, filed Shoemaker, R. et al., Public Soybean EST Project, on Jul. 21, 1998. XP-002129969. EMBL Database Accession No.: AUO68686, Jun. 7, 1999, (51) Int. Cl." ........................... C12P21/06; C12N 9/00; Sasaki, T. et al., XP-002129970. C12N 1/20; C12N 15/00; CO7H 21/04 Yunxia Wang et al., Plant Physiol, vol. 97:847-848, 1991, (52) U.S. Cl. ............................... 435/193; 435/4; 435/6; Cloning and Nucleotide Sequence of a Complementary 435/69.1; 435/183; 435/252.3; 435/320.1; DNA Encoding 3-Deoxy-D-arabino-Heptulosonate 536/23.2; 536/23.6 7-Phosphate Synthase from Tobacco. (58) Field of Search ............................... 435/4, 6, 69.1, Brian Keith et al., PNAS, vol. 88:8821-8825, Oct. 1991, 435/183, 193, 252.3, 320.1; 536/23.2, 23.6 Differential induction of 3-deoxy-D-arabino-heptu loSonate 7-phosphate Synthase genes in Arabidopsis (56) References Cited thaliana by wounding and pathogenic attack. Jianmin Zhao et al., Plant Phys., vol. 100:1075–1076, 1992, U.S. PATENT DOCUMENTS Cloning and Sequencing of a Second cDNA Encoding 5,187,071 A 2/1993 Fischer et al. 3-Deoxy-D-arabino-Heptulosonate 7-Phosphate Synthase from Solanum tuberosum L. FOREIGN PATENT DOCUMENTS National Center for Biotechnology Information General EP O 608 722 A1 8/1994 Identifier No. 2398679, Sep. 12, 1997, Wind, J.C. et al., EP O 832 978 A2 4/1998 Three differentially expressed 3-deoxy-D-arabino-heptu WO WO95/33843 12/1995 loSonate 7-phsophate Synthase genes in Morinda citrifolia. WO WO 97/26366 7/1997 National Center for Biotechnology Information General WO WO 98/03661 1/1998 Identifier No. 2546988, Wind, J.C. et al., Three differentially expressed 3-deoxy-D-arabino-heptuloSonate 7-phsophate OTHER PUBLICATIONS Synthase genes in Morinda citrifolia. Dyer et al. Accession A35016. Jul. 20, 1990.* (Continued) Attwood et al. Which craft is best in bioinformatics? Com put. Chem. 2001, vol. 25(4), pp. 329-339.* Primary Examiner Manjunath N. Rao Ponting, C.P. ISSues in predicting protein function from ASSistant Examiner-Christian L. Fronda sequence. Brief. Bioinform. Mar. 2001, vol. 2(1), pp. (57) ABSTRACT 19-29.* This invention relates to an isolated nucleic acid fragment EMBL Database Accession No. Y14797.1, Wind, J.C. et al., encoding a DAHP synthetase. The invention also relates to Jan. 1, 1998, Three differentially expressed 3-deoxy-D-ara the construction of a chimeric gene encoding all or a portion bino-heptuloSonate 7-phosphate Synthase genes in Morinda of the DAHP synthetase, in Sense or antisense orientation, citrifolia, XP-002129965. wherein expression of the chimeric gene results in produc EMBL Database Accession No. AF012864, Batz, O. et al., tion of altered levels of the DAHP synthetase in a trans 09-11-197, Extensive reprogramming of cellular metabo formed host cell. lism by fungal elicitor of infection in parsley Suggests a new perception of defense-related genes, XP-002129966. 7 Claims, No Drawings US 6,911,331 B2 Page 2 OTHER PUBLICATIONS EMBL Sequence Library Data Accession No.: AA586083, Sep. 13, 1997, Newman, T. et al., Genes galore: a summary National Center for Biotechnology Information General of methods for accessing results from large-Scale partial Identifier No. 584777, Dyer, W.E. et al., A cDNa encoding Sequencing of anonymous Arabidopsis cDNa clones. 3-deoxy-D-arabino-heptuloSonate 7-phosphate Synthase from Solanum tuberosum L. Gary Millar et al., FEBS Letters, vol. 200(1):Nov. 17, 1986, National Center for Biotechnology Information General The complete amino acid Sequence of 3-dehydroquinate Identifier No. 114193, May 30, 2000, Wang, Y. et al., synthase of Escherichia coli K-12. Cloning and nucleotide Sequence of a complementary DNA National Center for Biotechnology Information General encoding 3-deoxy-D-arabino-heptuloSonate 7-phosphate Identifier No. 114181, May 30, 2000, Millar, G. et al., The Synthase from tobacco. complete amino acid Sequence of 3-dehydroquinate Syn Gloria K. Muday et al., Plant Phys., vol. 98:496-500, 1992, thase of Escherichia coli K-12. Wounding Induces One of Two isoenzymes of 3-Deoxy EMBL Sequence Library Data Accession No.: P34002, Feb. D-arabino-Heptulosonate 7-Phosphate Synthase in 1, 1994, Martin, P. R. et al., Characterization of pilo, a nw Solanum tuberoSun L. gene required for the biogenesis of type 4 fimbriae in Robert J. Ganson et al., Plant Phys., vol. 82:203-210, 1986, Pseudomonas aeruginosa. The Two-isozyme System of 3-Deoxy-D-arabino-Heptu losonate 7-Phosphate Synthase in Nicotiana SilveStris and Nicholas Nikolaides et al., Tetrahedron Letters, vol. Other Higher Plants. 30(12): 1461-1464, 1989, Design and Synthesis of Substrate PR. Srinivasan et al., J. Biol. Chem., vol. 234:716–722, Analogs for the Inhibition of Dehydrogquinate Synthase. 1959, 2-Keto-3-deoxy-D-arabo-heptonic Acid 7, Phos John W. Frost et al., Biochemistry, vol. 23:4470–4475, 1984, phate Synthetase. Dehydroquinate Synthase from Escherichia coli: Purifica EMBL Sequence Library Data Accession No.: AI731017, tion, Cloning, and Construction of Overproducers of the Jun. 12, 1999, Blewitt, M. et al., ESTs from Developing Enzyme. Cotton Fiber. D. L. Pomplianao et al., J. Am. Chem. Soc., vol. EMBL Sequence Library Data Accession No. AI728073, 111:1866–1871, 1989, Probing Lethal Metabolic Pekturba Jun. 12, 1999, Blewitt, M. et al., ESTs from Developing tions in Plants with Chemical Inhibition of Dehdyroquinate Cotton Fiber. Synthase. EMBL Sequence Library Data Accession No.: AI489566, Genbank Accession No.: AIO65473, Jul. 24, 1998, Schutz, Mar. 17, 1999, Alcala, J. et al., Generation of STSs from K. et al., Expressed Sequence tags from Z. mayS. Tomato Carpel Tissue. Stephen Bornemann et al., Journ. of Biol. Chem., vol. EMBL. Sequence Library Data Accession No.: AIO65473, 270(39):22811-22815, 1995, Escherichia coli Chorismate Jul. 27, 1998, Schutz, K. et al., Expressed Sequence tags Synthase Catalyzes the Conversion of from Z. mayS. (6S)-6-Fluoro-50enolypyruvylshikimate-3-phosphate to EMBL Sequence Library Data Accession No.: AI637200, 6-Fluorochorismate. Apr. 27, 1999, Walbot, V., Maize ESTs from various cDNA EMBL Sequence Library Data Accession No.: C72774, Sep. libraries sequenced at Stanford University. 19, 1997, Sasaki, T. et al., Rice cDNA from Panicle at EMBL Sequence Library Data Accession No.: AU56551, Flowering Stage. Sasaki, T. et al., Rice cDNA from mature leaf. EMBL Sequence Library Data Accession No.: AA750226, Jan. 21, 1998, Nahm, B. H. et al., Large-scale Sequencing Markus Bischoff et al., Plant Mol. Biol., vol. 31:69–76, Analysis of STSs from Rice Immature Seed. 1996, Cloning of a cDNA encoding a 3-dehydrogguinate Andreas Schaller et al., Journ. of Biol. Chem., vol. Synthase from a higher plant, and analysis of the organ-spe 256(32):21434–21438, 1991, Molecular Cloning and Analy cific and elicitor-induced expression of the corresponding sis of cDNA Coding for Chorismate Synthase from the gene. Higher Plant Corydalis Sempervirens Pers. Craig M. Stephens et al., ESSential Cysteines in Jorn Gorlach et al., Plant Mol. Biol., vol. 23:707-716, 1993, 3-Deoxy-D-arabino-heptulosonate-7-phosphate Synthase Differential expression of tomoato (LCopersicon esculentum from Escherichia coli, J. Biol. Chem., Mar. 25, 1992, vol. L.) genes encoding Shikimate pathway isoenzymes. II. Cho 267, No. 9, pp. 5762-5767. rismate Synthase. Prem S. Subramaniam et al., Substrate Ambiguity of Genbank Accession No.: L33595, Jul. 11, 1994, Lim, C.O. 3-Deoxy-D-manno-Octulosonate 8-Phosphate Synthase et al., Expressed Sequence tags of Chinese cabbage flower from Neisseria gonorrhoeae in the Context of its Member bud cDNA. ship in a Protein Family Containing a Subset of 3-Deoxy Genbank Accession No.: AA586083, Sep. 11, 1997, New D-arabino-Heptulosonate 7-Phosphate Synthases, J.
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