USOO691.1331 B2 (12) United States Patent (10) Patent No.: US 6,911,331 B2 Famodu et al. (45) Date of Patent: Jun. 28, 2005 (54) CHORISMATE BIOSYNTHESIS ENZYMES William E. Dyer et al., Journ. of Biol Chem., vol. 265 1608–1614, 1990, A cDNA Encoding 3-Deoxy-D-arabino (75) Inventors: Omolayo O. Famodu, Newark, DE heptulosonate 7-Phosphate Synthase from Solanum tubero (US); William D. Hitz, Wilmington, sum L., XP-002129967. DE (US); Jonathan E. Lightner, Jorn Gorlach et al., Plant Mol. Biol., vol. 23:697–706, 1993, Mulino, OR (US) Differential expression of tomato (LycopersicOn eSculentum L.) genes encoding Shikimate pathway isoenzymes. I. (73) Assignee: E. I. du Pont de Nemours and 3-Deoxy-D-arabino-heptuloSonate 7-phosphate Synthase, Company, Wilmington, DE (US) XP-002121067. James D. Jones et al., Plant Physiol., vol. 108(4): 1413-1421, (*) Notice: Subject to any disclaimer, the term of this 1995, Impaired Wound Induction of patent is extended or adjusted under 35 3-Deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) U.S.C. 154(b) by 27 days. Synthase and Altered Stem Development in Transgenic Potato Plants Expressing a DAHP Synthase Antisense Con (21) Appl. No.: 10/105,729 struct, XP-002121068. Klaus M. Herrmann, Plant Physiol., vol. 107:Jul. 12, 1995. (22) Filed: Mar 22, 2002 The shikimate Pathway as an Entry to Aromatic Secondary (65) Prior Publication Data Metabolism, XP-002121069. EMBL Database Accession No.: AI677182, May 25, 1999, US 2003/0003557 A1 Jan. 2, 2003 Walbot, V., Maize ESTs from various cDNA libraries sequenced at Stanford University, XP-002121070. Related U.S. Application Data EMBL Database Accession No.: AI615213, Apr. 26, 1999, Walbot, V., Maize ESTs from various cDNA libraries (63) Continuation-in-part of application No. 09/743,210, filed on sequenced at Stanford University, XP-002129968. Feb. 23, 2001, now abandoned. (60) Provisional application No. PCT/US99/16352, filed on Jul. EMBL Database Accession No.: AI443687, Mar. 16, 1999, 20, 1999, and provisional application No. 60/093.611, filed Shoemaker, R. et al., Public Soybean EST Project, on Jul. 21, 1998. XP-002129969. EMBL Database Accession No.: AUO68686, Jun. 7, 1999, (51) Int. Cl." ........................... C12P21/06; C12N 9/00; Sasaki, T. et al., XP-002129970. C12N 1/20; C12N 15/00; CO7H 21/04 Yunxia Wang et al., Plant Physiol, vol. 97:847-848, 1991, (52) U.S. Cl. ............................... 435/193; 435/4; 435/6; Cloning and Nucleotide Sequence of a Complementary 435/69.1; 435/183; 435/252.3; 435/320.1; DNA Encoding 3-Deoxy-D-arabino-Heptulosonate 536/23.2; 536/23.6 7-Phosphate Synthase from Tobacco. (58) Field of Search ............................... 435/4, 6, 69.1, Brian Keith et al., PNAS, vol. 88:8821-8825, Oct. 1991, 435/183, 193, 252.3, 320.1; 536/23.2, 23.6 Differential induction of 3-deoxy-D-arabino-heptu loSonate 7-phosphate Synthase genes in Arabidopsis (56) References Cited thaliana by wounding and pathogenic attack. Jianmin Zhao et al., Plant Phys., vol. 100:1075–1076, 1992, U.S. PATENT DOCUMENTS Cloning and Sequencing of a Second cDNA Encoding 5,187,071 A 2/1993 Fischer et al. 3-Deoxy-D-arabino-Heptulosonate 7-Phosphate Synthase from Solanum tuberosum L. FOREIGN PATENT DOCUMENTS National Center for Biotechnology Information General EP O 608 722 A1 8/1994 Identifier No. 2398679, Sep. 12, 1997, Wind, J.C. et al., EP O 832 978 A2 4/1998 Three differentially expressed 3-deoxy-D-arabino-heptu WO WO95/33843 12/1995 loSonate 7-phsophate Synthase genes in Morinda citrifolia. WO WO 97/26366 7/1997 National Center for Biotechnology Information General WO WO 98/03661 1/1998 Identifier No. 2546988, Wind, J.C. et al., Three differentially expressed 3-deoxy-D-arabino-heptuloSonate 7-phsophate OTHER PUBLICATIONS Synthase genes in Morinda citrifolia. Dyer et al. Accession A35016. Jul. 20, 1990.* (Continued) Attwood et al. Which craft is best in bioinformatics? Com put. Chem. 2001, vol. 25(4), pp. 329-339.* Primary Examiner Manjunath N. Rao Ponting, C.P. ISSues in predicting protein function from ASSistant Examiner-Christian L. Fronda sequence. Brief. Bioinform. Mar. 2001, vol. 2(1), pp. (57) ABSTRACT 19-29.* This invention relates to an isolated nucleic acid fragment EMBL Database Accession No. Y14797.1, Wind, J.C. et al., encoding a DAHP synthetase. The invention also relates to Jan. 1, 1998, Three differentially expressed 3-deoxy-D-ara the construction of a chimeric gene encoding all or a portion bino-heptuloSonate 7-phosphate Synthase genes in Morinda of the DAHP synthetase, in Sense or antisense orientation, citrifolia, XP-002129965. wherein expression of the chimeric gene results in produc EMBL Database Accession No. AF012864, Batz, O. et al., tion of altered levels of the DAHP synthetase in a trans 09-11-197, Extensive reprogramming of cellular metabo formed host cell. lism by fungal elicitor of infection in parsley Suggests a new perception of defense-related genes, XP-002129966. 7 Claims, No Drawings US 6,911,331 B2 Page 2 OTHER PUBLICATIONS EMBL Sequence Library Data Accession No.: AA586083, Sep. 13, 1997, Newman, T. et al., Genes galore: a summary National Center for Biotechnology Information General of methods for accessing results from large-Scale partial Identifier No. 584777, Dyer, W.E. et al., A cDNa encoding Sequencing of anonymous Arabidopsis cDNa clones. 3-deoxy-D-arabino-heptuloSonate 7-phosphate Synthase from Solanum tuberosum L. 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