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Poster Abstracts CORE Metadata, citation and similar papers at core.ac.uk Provided by Edinburgh Research Explorer Edinburgh Research Explorer Poster 033 Follicle homing antigen presenting cells modulate TH2 bias Citation for published version: Bradford, B, Donaldson, D, Else, K & Mabbott, N 2014, 'Poster 033 Follicle homing antigen presenting cells modulate TH2 bias: Conditional knockout of CXCR5 on CD11c+ cells prevents protective TH2 response following T. muris infection' 9th European-Mucosal-Immunology-Group Meeting, Glasgow, United Kingdom, 1/10/14 - 12/10/14, . DOI: 10.13140/2.1.2667.2644 Digital Object Identifier (DOI): 10.13140/2.1.2667.2644 Link: Link to publication record in Edinburgh Research Explorer Document Version: Early version, also known as pre-print General rights Copyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policy The University of Edinburgh has made every reasonable effort to ensure that Edinburgh Research Explorer content complies with UK legislation. If you believe that the public display of this file breaches copyright please contact [email protected] providing details, and we will remove access to the work immediately and investigate your claim. Download date: 05. Apr. 2019 IMMUNOLOGY ABSTRACTS Poster Abstracts 001 002 Cryptosporidium parvum subverts the host innate immune Faecalibacterium prausnitzii strain HTF-F and its extracellular response through manipulation of CRAMP expression during polymeric matrix attenuate clinical parameters in DSS-induced neonatal infection colitis W. Guesdon1, T. Pezier1, F. Drouet1, S. Menard2, F. Laurent1 & O. Rossi1, M. Schwarzer2, T. Hudcovic2, D. Srutkova2, S. Duncan3, S. Lacroix-Lamande1 H. Flint3, J. Samsom4, H. Harmsen5 & J. Wells1 1Animal Health, UMR1282 Inra-Universite, Nouzilly, France, 1Host-Microbe Interactomics, Wageningen University, Wageningen, 2Toxalim, UMR 1331 INRA/INP/UPS, Toulouse, France Netherlands, 2Academy of Sciences of the Czech Republic, Prague, Czech Republic, 3Rowett Institute of Nutrition and Health, University Due to the immaturity of their immune system, neonates are highly Aberdeen, Aberdeen, UK, 4Department of Pediatrics, Erasmus Medical sensitive to intestinal infections. During the neonatal period, antimi- Center, Sophia Children’s Hospital, Rotterdam, Netherlands, crobial peptide (AMP) expression differs substantially from that of 5Department of Medical Microbiology, UMCG, Groningen, Netherlands adults as this is the case for the cathelicidin-related antimicrobial peptide CRAMP expressed preferentially in the neonatal period while Introduction: A decrease in the abundance and biodiversity of intes- conversely other AMPs such as Reg3c are expressed later in life. tinal bacteria within the Firmicutes phylum has been associated with Among enteric neonatal diseases, Cryptosporidiosis is a zoonotic inflammatory bowel disease (IBD). In particular, the anti-inflamma- disease and is highly prevalent in in young infant less than 5 years tory bacterium Faecalibacterium prausnitzii, member of the Firmi- old in underdeveloped country and in neonatal ruminants world- cutes phylum and one of the most abundant species in healthy wide. Cryptosporidium parvum is the etiological agent of this diar- human colon, is underrepresented in the microbiota of IBD patients. rheal disease and infects exclusively epithelial cells. Innate immunity Method: In this study we investigated the capacity of F. prausnitzii is important to control the acute phase of infection in neonates with strain A2-165, the biofilm forming strain HTF-F and the extracellular dendritic cells and IFNc playing a major role. Antimicrobial peptides polymeric matrix (EPM) isolated from strain HTF-F, to suppress inflam- are important contributors of innate immunity, but the role of mation in the mouse dextran sodium sulphate (DSS) colitis model. CRAMP, which is elevated in the intestine of neonates has never Results: The two F. prausnitzii strains have anti-inflammatory effects been investigated during Cryptosporidiosis so far. in the DSS colitis model.F. prausnitzii HTF-F is more effective than In this work, we used the neonatal murine model of cryptosporid- A2-165 partly because of the immune-regulating properties of the iosis and unlike all the other antimicrobial peptides analyzed EPM. The immunomodulatory effects of the EPM are mediated CRAMP expression in the intestinal epithelial cells was significantly through the TLR2-dependent modulation of IL-12 and IL-10 cyto- reduced during infection. This reduced CRAMP expression is inde- kine production in antigen presenting cells. Both F. prausnitzii HTF- pendent of IFNg, a cytokine strongly produced during infection but F and the EPM may have a therapeutic use in IBD. also Myd88 and gut flora independent. When C. parvum infected neonatal mice orally received exogenous CRAMP to compensate the reduced expression of this AMP, the parasitic load of neonates was 003 significantly decreased. In addition, when free parasites were in Roseburia hominis regulates immunity through TLR-5 dependent direct contact with CRAMP, this AMP affects the viability of spor- responses ozoites, the first free infectious form of this parasite. All together, these data suggest that C. parvum induces the reduction of CRAMP A.M. Patterson, E. Monnais, A.G.P. Coutts, N.H. McKenzie, expression to escape the anti-parasiticidal effect of CRAMP. The E. Logan, M. Delday, I.E. Mulder & G. Grant molecular mechanism by which the parasite subverts epithelial- Gut Immunology, Life Sciences Innovation Building, Cornhill Road, derived CRAMP production is currently under investigation. Aberdeen, UK Commensal bacteria play a fundamental role in maintaining immune homeostasis a complex balance between immune stimulation and appro- priate responsiveness and immune suppression and tolerance. Break- down of these immune regulatory processes lead to chronic inflammation associated with intestinal dysbiosis. The immune modulat- ing properties of Roseburia hominis, a flagellate gut anaerobe, were investigated in colonised conventional and colitic mouse models. Oral administration of Roseburia hominis to conventional mice with colitis caused by Dextran Sodium Sulphate altered intestinal immune responses, attenuated gut inflammation and reduced the severity of colitis. This protection was associated with a modulation and expansion of the T reg- ulatory cell population triggered by R. hominis. The role of flagellin in directing these immune responses was investigated in vitro and in vivo. Part funded by RESAS, Scotland and part funded by GT Biologics. ª 2014 The Authors. © 2014 John Wiley & Sons Ltd, Immunology, 143 (Suppl. 1), 12–42 Abstracts 13 004 Stimulation of adenotonsillar MNC by CpG-DNA significantly A specific tolerance inducing vector for therapeutic treatment of increased TFH number and that was correlated with HA-specific autoimmune diseases antibody production following influenza antigen stimulation. Co-incubation with purified plasmacytoid dendritic cells significantly C. Hansson, K. Schon€ & N. Lycke enhanced the CpG-DNA-mediated antibody production. Microbiology and Immunology, Gothenburg University, Gothenburg, Conclusion: A prominent number of TFH were shown in human Sweden NALT especially in children and that were highly active in B cell- Introduction: We have developed a novel platform for intranasal treat- help for antibody production. CpG-DNA promoted NALT TFH cells ment of autoimmune diseases in which ADP-ribosylation determines which correlated with the enhancement of HA-specific antibody pro- whether immunity or tolerance is induced. Hence, cholera toxin duction. To enhance vaccine immunogenicity by promoting TFH A1-subunit based immunomodulation through the CTA1-peptide-DD function may be an effective vaccination strategy. fusion protein promotes enhancement, while inactive mutants induce suppression. Whereas the target population after intranasal administra- tion of both constructs was CD103+CD11blow DC’s, both active and inactive mutants induced strong CD4 T cell priming, but they differen- 006 tially affected CD4 T cell differentiation. This way inactive mutant con- A20/TNFAIP3 as a brake on intestinal inflammation and structs generated regulatory CD4 T-cells producing IL-10 and effectively tumorigenesis preventing experimental autoimmune encephalitis (EAE) and collagen- L. Vereecke1, S.V. Silva2, T. Billiet3, J.H. van Es4, C. McGuire1, induced arthritis (CIA), when carrying relevant peptides. Targeted DCs K. Slowicka1, M. Sze1, M. van den Born4, G.D. Hertogh5, expressed low levels of CD80, CD86 and CD40, while, by contrast, H. Clevers4, J. Raes6, P. Rutgeerts3, S. Vermeire3, R. Beyaert1 & ADP-ribosylating CTA1-peptide-DD constructs gave significantly G. van Loo1 enhanced co-stimulation. Suppression was global in that regulatory 1Inflammation Research Centre, Technologiepark 927, Ghent, Belgium, T-cells following treatment were able to suppress adoptively transferred 2Department of Microbiology and Immunology, Rega In, Belgium, € naıve CD4 T cells and could subsequently maintain tolerance. 3Department of Clinical and Experimental Medicine, Leuven, Belgium, Conclusion: The use of specifically tolerance-inducing fusion pro- 4Hubrecht Institute for Developmental Biology and S, Utrecht, teins may be a way forward in the search of effective treatments Netherlands,
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