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® Product Catalogue 2017 © Liofilchem® s.r.l. Liofilchem Roseto degli Abruzzi, Italy Est. 1983 Clinical and Industrial Microbiology Ready culture media in 55 mm contact plates Individually packed in transparent blister # Product with minimum order Description Packaging Ref. Baird Parker Agar + Neutralizing 20 plates 15331 Selective medium for Staphylococcus aureus isolation with inactivation of disinfectants. Cetrimide Agar E.P. 20 plates 15332 Selective medium for Pseudomonas aeruginosa isolation. Cetrimide Agar + Neutralizing E.P. 20 plates 15386 # Selective medium for Pseudomonas aeruginosa isolation with inactivation of disinfectants. Legionella BCYE Agar ISO 11731 20 plates 15334 Non-selective medium for Legionella spp. isolation. ISO 11731-2 Legionella GVPC Agar ISO 11731 20 plates 15376 Selective medium for Legionella spp. isolation. ISO 11731-2 MacConkey Agar E.P. 20 plates 15329 Selective medium for gram-negatives isolation. Mannitol Salt Agar EP, USP, JP 20 plates 15328 Selective medium for staphylococci isolation. m-Faecal Coliform Agar 20 plates 15368 Selective medium for fecal coliforms isolation and enumeration. Plate Count Agar ISO 4833 20 plates 15325 Medium for surfaces and air total bacterial count. Plate Count Agar + Neutralizing ISO 4833 20 plates 15336 Medium for surfaces total bacterial count with inactivation of disinfectants. Plate Count Agar + Neutralizing (Irradiated) ISO 4833 20 plates 15336S Medium for surfaces total bacterial count with inactivation of disinfectants. Plate Count Agar + TTC + Neutralizing ISO 4833 20 plates 15360 Medium for surfaces total bacterial count with inactivation of disinfectants. Rose Bengal Agar + CAF APHA 20 plates 15374 Selective medium for yeasts and moulds isolation and enumeration. Rose Bengal Agar + CAF + Neutralizing 20 plates 15385 # Selective medium for yeasts and moulds isolation and enumeration. R2 Agar E.P. 20 plates 15354 # Medium for heterotrophic microorganisms count in drinking water. Sabouraud Dextrose Agar E.P. 20 plates 15327 Medium for yeasts and moulds isolation. Sabouraud Dextrose Agar + Lactamator 500 IU + Neutralizing (Irradiated) 20 plates 15345S Medium for yeasts and moulds isolation with inactivation of β-lactam antibiotics and disinfectants. Sabouraud Dextrose Agar + 0.1% Penase + Neutralizing (Irradiated) 20 plates 15333S Medium for yeasts and moulds isolation with inactivation of disinfectants and penicillins. Sabouraud Agar + Neutralizing (Irradiated) 20 plates 15323S Medium for yeasts and moulds isolation with inactivation of disinfectants. 400 plates 15323KS Sabouraud CAF Agar (Irradiated) 20 plates 15380S Selective medium for yeasts and moulds isolation. Sabouraud CAF Agar + Neutralizing 20 plates 15365 Selective medium for yeasts and moulds isolation with inactivation of disinfectants. Slanetz-Bartley Agar + Neutralizing 20 plates 15362 Selective medium for fecal streptococci enumeration and isolation with inactivation of disinfectants. Tryptic Soy Agar (Irradiated) E.P. 20 plates 15340S Medium for surfaces and air total bacterial count. Tryptic Soy Agar + Neutralizing (Irradiated) E.P. 20 plates 15339S Medium for surfaces and air total bacterial count with inactivation of 400 plates 15339KS disinfectants. 46 ® Product Catalogue 2017 © Liofilchem® s.r.l. Liofilchem Roseto degli Abruzzi, Italy Est. 1983 Clinical and Industrial Microbiology Ready culture media in 55 mm contact plates Individually packed in transparent blister # Product with minimum order Description Packaging Ref. Tryptic Soy Agar + 0.1% Penase + Neutralizing 20 plates 15330 # Medium for surfaces and air total bacterial count with inactivation of disinfectants and penicillins. Tryptic Soy Agar + 0.1% Penase + Neutralizing (Irradiated) 20 plates 15330S # Medium for surfaces and air total bacterial count with inactivation of disinfectants and penicillins. Tryptic Soy Agar + Penase + Cephalosporinase + Neutralizing 20 plates 15343 # Medium for microbial detection with inactivation of β-lactam antibiotics and disinfectants. Tryptic Soy Agar + Lactamator 500 UI + Neutralizing (Irradiated) 20 plates 15341S # Irradiated medium for non fastidious bacteria growth with inactivation of β- lactam antibiotics and disinfectants. Violet Red Bile Glucose Agar ISO 21528-2 20 plates 15375 Selective medium for enterobacteria isolation and enumeration. E.P. Violet Red Bile Lactose Agar ISO 4832 20 plates 15326 Selective medium for coliforms isolation and enumeration. Violet Red Bile Glucose Agar + Neutralizing ISO 21528-2 20 plates 15382 Selective medium for Enterobacteriaceae isolation and enumeration with inactivation of disinfectants. Violet Red Bile Lactose Agar + Neutralizing ISO 4832 20 plates 15361 Selective medium for coliforms isolation and enumeration with inactivation of disinfectants. Ready culture media in 55 mm contact plates Packed in two piles of 10 plates # Product with minimum order Description Packaging Ref. Plate Count Agar + Neutralizing ISO 4833 20 plates 15336L # Medium for surfaces total bacterial count with inactivation of disinfectants. Plate Count Agar + TTC + Neutralizing ISO 4833 20 plates 15360L # Medium for surfaces total bacterial count with inactivation of disinfectants. Tryptic Soy Agar + Neutralizing (Irradiated) E.P. 20 plates 15739S # Medium for surfaces and air total bacterial count with inactivation of disinfectants. Violet Red Bile Glucose Agar ISO 21528-2 20 plates 15375L # Selective medium for enterobacteria isolation and enumeration. E.P. Violet Red Bile Lactose Agar ISO 4832 20 plates 15326L # Selective medium for coliforms isolation and enumeration. 47 ® Product Catalogue 2017 © Liofilchem® s.r.l. Liofilchem Roseto degli Abruzzi, Italy Est. 1983 Clinical and Industrial Microbiology Ready culture media in 60 mm petri dishes # Product with minimum order Individually packed in transparent blister Description Packaging Ref. MEMBRANE DISC FILTERS Mixed cellulose ester membrane for the isolation and enumeration of bacteria by Membrane Filter Technique. 47 mm - 0.45 μm - white 100 filters 97000 47 mm - 0.45 μm - black 100 filters 97013 47 mm - 0.22 μm - white 100 filters 97014 Aeromonas Agar 20 plates 163622 # Selective medium for Aeromonas spp. isolation. Baird Parker Agar ISO 6888-1 20 plates 163512 Selective medium for Staphylococcus aureus isolation. Bile Aesculin Azide Agar ISO 7899-2 20 plates 163572 Selective medium for group D streptococci isolation. C-EC MF Agar 20 plates 163862 Chromogenic and fluorogenic medium for isolating and detecting Escherichia coli and other coliforms in water. Chromatic™ EC X-Gluc Agar 20 plates 163722 # Chromogenic medium for the detection of E. coli in water and foodstuffs. Iron Sulfite Agar 20 plates 163372 # Medium for thermophilic anaerobes determination. Legionella Agar (GVPC) ISO 11731 20 plates 163392 Selective medium for Legionella spp. isolation. ISO 11731-2 Legionella MWY Agar ISO 11731 20 plates 163382 # Selective medium for Legionella spp. isolation. ISO 11731-2 MacConkey Agar E.P. 20 plates 163542 Selective medium for enterobacteria isolation. m-CP Agar 98/83/EC 20 plates 163612 Selective medium for Clostridium perfringens isolation from water. m-Faecal Coliform Agar APHA 20 plates 163442 Selective medium for fecal coliforms isolation and enumeration. M-Green Yeast & Mould Agar 20 plates 163642 # Selective medium for detection and count of yeasts and moulds in beverages. M-Green Yeast & Mould Agar + CAF 20 plates 163812 # Selective medium for detection and count of yeasts and moulds in beverages. M-Green Yeast & Mould Agar pH modified 20 plates 163842 # Selective medium for detection and count of yeasts and moulds in beverages. Mycosel Agar 20 plates 163412 # Selective medium for pathogenic fungi isolation. Orange Serum Agar 20 plates 163732 # Medium for the isolation and enumeration of microorganisms associated with spoilage of citrus fruits and derivatives. Plate Count Agar ISO 4833 20 plates 163452 Medium for bacterial count in water and foodstuffs determination. Cetrimide Agar E.P. 20 plates 163472 Selective medium for Pseudomonas aeruginosa isolation. Pseudomonas CN Agar ISO 12780 20 plates 163592 Selective medium for Pseudomonas spp. isolation. R2 Agar E.P. 20 plates 163672 Medium for heterotrophic microorganisms count in drinking water. Sabouraud Dextrose Agar E.P. 20 plates 163402 Medium for yeasts and moulds isolation. Slanetz-Bartley Agar (m-Enterococcus A.) ISO 7899-2 20 plates 163462 Selective medium for fecal streptococci enumeration and isolation. SPS Agar APHA 20 plates 163522 Medium for C. perfringens and sulfite reducing bacteria isolation and count. Streptococcal KF+TTC Agar APHA 20 plates 163482 Selective medium for fecal streptococci isolation and enumeration. 48 ® Product Catalogue 2017 © Liofilchem® s.r.l. Liofilchem Roseto degli Abruzzi, Italy Est. 1983 Clinical and Industrial Microbiology Ready culture media in 60 mm petri dishes # Product with minimum order Individually packed in transparent blister Description Packaging Ref. TBX Agar ISO 16649 20 plates 163652 Selective chromogenic medium for detecting and enumerating E. coli in food. Tergitol Agar ISO 9308-1 20 plates 163562 Selective medium for coliforms enumeration and identification. Tergitol + TTC Agar ISO 9308-1 20 plates 163492 Selective medium for coliforms enumeration and identification. Tryptone Glucose Extract Agar + TTC 20 plates 163742 # Medium for cultivating and enumerating microorganisms in water and dairy products. Wort Agar 20 plates 163802 Medium for the cultivation, isolation and enumeration or enrichment of fungi, especially
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    ISSN: 08526834 | E-ISSN: 2337-389X Journal of BIOLOGICAL RESEARCHES Volume 24| No. 2| June| 2019 Original Article Profile of microbial community of naturally fermented Sumbawa mare’s milk using next-generation sequencing Yoga Dwi Jatmiko*, Irfan Mustafa, Tri Ardyati Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, Indonesia Abstract This study aimed to investigate the bacterial and fungal/yeast diversity in naturally fermented Sumbawa mare’s milk through a next-generation se- quencing approach, and evaluate the quality of fermented mare’s milk based on the presence of pathogenic or undesirable microorganisms. Microbial density determined using plate count agar (total aerobic bacteria), de Man Rogosa Sharpe agar (Lactobacillus), M17 agar (Lactococcus) and yeast peptone dextrose agar supplemented with streptomycin 50 ppm (yeast). Nutritional content and acidity level of each fermented milk sample were also evaluated. Genomic DNA was extracted using FastDNA Spin (MPBIO). The total gDNA was further analyzed using illumina high-throughput se- quencing (paired-end reads), and the sequence results were analysed using QIIME v.1.9.1 to generate diversity profiles. The difference in nutrient content of mare’s milk was thought to affect the density and diversity of microbes that were able to grow. Fermented mare’s milk samples from Sum- bawa had the highest bacterial diversity compared to samples from Bima and Dompu. However, fermented mare’s milk from Dompu had the best quality which was indicated by the absence of bacteria that have the potential to be pathogenic or food spoilage, such as members of the Enterobacte- riaceae family (Enterobacter, Klebsiella and Escherichia-Shigella) and Pseudomonas.
  • The Use of a Single Growth Medium for Environmental Monitoring Of

    The Use of a Single Growth Medium for Environmental Monitoring Of

    European Journal of Parenteral & Pharmaceutical Sciences 2016; 21(2): 50-55 © 2016 Pharmaceutical and Healthcare Sciences Society The use of a single growth medium for environmental monitoring of pharmacy aseptic units using tryptone soya agar with 1% glucose John Rhodes1*, Jennifer Feasby1, Wayne Goddard1, Alison Beaney2 and Mike Baker3 1 North Tees and Hartlepool NHS Foundation Trust, Stockton-on-Tees, UK 2 Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle-upon-Tyne, UK 3 Pharma Quality Consulting, Cheshire, UK The suitability of tryptone soya agar, Sabouraud dextrose agar and tryptone soya agar with 1% glucose plates for general environmental monitoring was compared. Plates were incubated at three different temperatures to assess an optimal temperature for growth. Results indicated that there are benefits from using tryptone soya agar with 1% glucose incubated at 25°C as an all-purpose medium for environmental monitoring. Key words: Tryptone soya agar, Sabouraud dextrose agar, tryptone soya agar with 1% glucose, environmental monitoring of aseptic rooms, settle plates, finger dabs. Introduction any test methods or suitable media. For cleanroom monitoring of UK National Health Service (NHS) aseptic services, more The choice of a microbiological growth medium is not simple. detailed guidance has been provided4 for environmental settle The Difco Manual has proven to be a comprehensive source of plate agars. It indicates that the media recommended is: information since the first edition appeared in 1927. The tenth edition was published in 1984 and found its way into most 1 “standardised on tryptone soya for bacterial count (this microbiology laboratories . The guide for the selection of will also detect yeasts and moulds to an extent) and culture media formed a 9-page table and contained many agars Sabouraud dextrose medium for the selective and broths for isolation, differentiation and propagation of determination of yeasts and moulds.” 4 different classes of micro-organisms.