DOCSLIB.ORG

Diversity Analysis, Identification, and Bioprospecting of Lactic Acid Bacteria (LAB) Isolated from Sumbawa Horse Milk

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Diversity Analysis, Identification, and Bioprospecting of Lactic Acid Bacteria (LAB) Isolated from Sumbawa Horse Milk BIODIVERSITAS ISSN: 1412-033X Volume 22, Number 6, June 2021 E-ISSN: 2085-4722 Pages: 3333-3340 DOI: 10.13057/biodiv/d220639 Diversity analysis, identification, and bioprospecting of Lactic Acid Bacteria (LAB) isolated from Sumbawa horse milk KHADIJAH ALLIYA FIDIEN1, BASO MANGUNTUNGI1, LINDA SUKMARINI2,, APON ZAENAL MUSTOPA2, LITA TRIRATNA2, FATIMAH3, KUSDIANAWATI1 1Department of Biotechnology, Faculty of Biotechnology, Universitas Teknologi Sumbawa. Jl. Raya Olat Maras, Moyo Hulu, Sumbawa 84371, West Nusa Tenggara, Indonesia 2Research Center for Biotechnology, Indonesian Institute of Sciences. Jl. Raya Jakarta-Bogor Km 46, Cibinong, Bogor 16911, West Java, Indonesia. Tel.: +62-21-8754587, Fax.: +62-21-8754588, email: [email protected] 3Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. Jl. Tentara Pelajar No. 3A, Cimanggu, Bogor 16111, West Java, Indonesia Manuscript received: 25 April 2021. Revision accepted: 24 May 2021. Abstract. Fidien KA, Manguntungi B, Sukmarini L, Mustopa AZ, Triratna L, Fatimah, Kusdianawati. 2021. Diversity analysis, identification, and bioprospecting of Lactic Acid Bacteria (LAB) isolated from Sumbawa horse milk. Biodiversitas 22: 3333-3340. Sumbawa horse milk has a probiotic potential because of the presence of Lactic Acid Bacteria (LAB). The LAB present in Sumbawa horse milk has been reported to have antimicrobial activities against pathogenic bacteria, including Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, and Vibrio cholerae. However, the potential of LAB from Sumbawa horse milk as antioxidant and antidiabetic is still unexplored. Studies related to the diversity of indigenous bacteria in Sumbawa horse milk based on metagenomic analysis have not been widely studied either. Therefore, this study aimed to determine the diversity of species of indigenous bacteria in Sumbawa horse milk and to identify LAB bioprospecting from Sumbawa horse milk. The diversity of indigenous bacterial species was investigated by the 16S rRNA gene-targeted metagenomic approach from bacterial DNA isolated from Sumbawa horse milk. The identification of LAB was also carried out by the 16S rRNA gene identification method. LAB bioprospecting on antioxidant activity was determined using the DPPH method, while the antidiabetic activity was measured using the α-glucosidase inhibition assay. The diversity analysis of indigenous bacteria based on 16S rRNA gene-based metagenomic revealed at least 7 phyla were relatively abundant in Sumbawa horse milk. The greatest abundance was shown by the phylum Proteobacteria (0.641%) and Firmicutes (0.327%). Enterococcus durans (39.01%) was the species that had the highest abundance in Sumbawa horse milk, followed by Lactococcus garvieae (30.13%) and Lactococcus lactis (19.85%). Moreover, based on the identification of the 16S rRNA gene, eight LAB isolates had similarities to bacterial strains, including Enterococcus faecium DSM 20477, E. faecium NBRC 100486, E. faecium ATCC 19434, E. durans 98D, E. faecalis ATCC 19433, E. faecalis NRBC 100480, Lactococcus lactis subsp. hordniae NBRC 100931 and L. garvieae JCM 10343 with similarity levels of more than 98%. In terms of LAB bioprospecting, the antioxidant assay showed the highest DPPH radical binding activity by L. garvieae L.22PR (43%). Meanwhile, the highest inhibitory activity of α-glucosidase was shown by E. faecium G.6PR (45%). Keywords: Bioprospection, diversity analysis, identification, metagenomics, Lactic Acid Bacteria, Sumbawa horse milk INTRODUCTION 2004). Studies on the microbial community and metagenome functional analysis in milk have been Sumbawa horse milk widely known as wild horse milk, conducted in human milk (Ward et al. 2013) and goat milk is a special commodity from Sumbawa Island, West Nusa (Zhang et al. 2017a). Tenggara, Indonesia. According to Sujaya et al. (2008a), The study on LAB carried out by Sujaya et al. (2008b) Sumbawa horse milk has probiotic potential. It has been revealed that the isolated LAB from Sumbawa horse milk reported that this milk could increase cellular immunity was predominantly belongs to the Lactobacilli. Several LAB against Salmonella thyphimurium infection (Reni et al. species that have been characterized include Lactobacillus 2013). The presence of lactic acid bacteria (LAB) in acidophilus, Lb. brevis, Lb. plantarum, and Lactococcus Sumbawa horse milk provides beneficial health effects, lactis (Antara et al. 2009). Setyowati et al. (2012) and such as growth modulation bacteria beneficial for health in Manguntungi et al. (2018) reported that LAB isolated from the human digestion (Sujaya et al. 2012). Investigations on Sumbawa horse milk had antimicrobial activity against the diversity of indigenous bacteria in Sumbawa horse milk pathogenic bacteria, including Staphylococcus epidermidis, are still limited to LAB and its metabolites (Sujaya et al. S. aureus, Escherichia coli, and Vibrio cholerae. However, 2008b; Setyowati et al. 2012; Nuraida 2015; Manguntungi to the best of our knowledge, there has been no study yet et al. 2018). Moreover, the method used to identify bacteria on bioprospecting of other than antimicrobial activities of present in this milk still depends on microbial cultivation. LAB from Sumbawa horse milk. Metagenomics is a functional analysis method based on Thus, in this study, 16S rRNA gene-based metagenomic microbial DNA sequences in the sample (Handelsman approach was employed to detect populations of 3334 BIODIVERSITAS 22 (6): 3333-3340, June 2021 indigenous species of bacteria found in Sumbawa horse slight modification. Bacterial cell pellet collection was milk. LAB identification from Sumbawa horse milk was performed by transferring 1.5 mL of the M17 broth culture also carried out to determine the isolated species. In to a microtube and centrifuge at 6,000 rpm, 4 °C for 10 addition, bioprospecting on antioxidant and antidiabetic min. The supernatant was then removed. The pellets were activities was also assessed to uncover further the potential suspended with 500 µL TE Buffer pH 8.0 containing 60 of LAB from Sumbawa horse milk. mg/mL lysozyme, then incubated at 37°C for 1 h. After incubation, 200 µL 10% SDS, 100 µL 5 M NaCl, and 80 µL 10% CTAB were added and the mixture was incubated MATERIALS AND METHODS at 60°C for 30 min (inverted every 10 min). A total of 500 µL chloroform was added and then centrifuged at 13,000 Metagenomic DNA isolation of Sumbawa horse milk rpm 4°C for 15 min. The supernatant was transferred into a The metagenomic DNA was isolated from horse milk new microtube and 0.6 x volume isopropanol was added, collected from the Penyaring Village, Sumbawa District, then incubated at -20°C for 2 h. After centrifugation at West Nusa Tenggara. Isolation was carried out following 13,000 rpm 4°C for 10 min, the supernatant was removed the manufacture’s instruction of ZymoBIOMICS™ DNA and the pellet was washed with 1 mL of 70% ethanol. The Miniprep Kit (Zymo Research Corp.) with a slight DNA pellet was then dried overnight before dissolved modification. Briefly, a sample of 5 mL of Sumbawa horse using 27 µL ddH2O and 3 µL RNase (1 mg/mL) and milk was put into a 15 mL falcon and centrifuged at 2,000 incubated at 37°C for 60 min. rpm, 4°C for 10 min. The supernatant was taken and centrifuged again at 6000 rpm, 4°C for 10 min. The pellet Polymerase Chain Reaction (PCR) amplification of 16S so collected was suspended with 750 μL Lysis Solution, rRNA gene then transferred into the Lysis Tubes. The resuspension Amplification of the 16S rRNA gene was performed was then vortexed for ≥ 20 minutes then centrifuged at using a thermal cycler. Each PCR reaction (50 µL) 10,000 rpm for 1 min. A total of 400 μL supernatant was containing: 2.5 µL of 100 ng DNA template; 1 µL for each transferred into a filter in a collection tube and centrifuged 10 mM primer; 1 µL My Taq DNA Polymerase (Bioline), at 8,000 rpm for 1 min. A total of 1200 μL DNA Binding 10 µL 5x My Taq Hs Red Buffer (Bioline), and 34.5 µL Buffer was added and mixed well. A total of 800 μL ddH2O. Oligonucleotides for bacterial PCR 16S rRNA sample was transferred into the column and centrifuged at include forward 8F primer (5'-AGA GTT TGA TCA TGG 10,000 rpm for 1 min. The supernatant was then discarded. CTC AG-3 ') and 15R reverse primer (5'-AAG GAG GTG This stage was carried out twice. After that, a total of 400 ATC CAA CCG CA-3'). The PCR conditions used were as μL Wash Buffer 1 was added to the column in a new follows: pre-denaturation for 5 minutes at 95°C, collection tube and centrifuged for 10 min, then the denaturation at 95°C for 1 min, annealing at 58°C for 1 supernatant was removed. A total of 700 μL Wash Buffer 2 min, an extension at 72°C for 30 sec, and a final extension was added to the column and centrifuged at 10,000 rpm for at 72°C for 7 min. PCR was carried out in 35 cycles. PCR 1 min then the supernatant was removed. A total of 200 μL products were electrophoresed on 1% agarose gel in TAE 1 Wash Buffer 2 was added back to the column on the x Running Buffer at 100 V and stained with ethidium Collection Tube and centrifuged at 10,000 rpm for 1 min. bromide. Electrophoresis gel was visualized with a UV-Vis The column was transferred into a 1.5 mL microtube, and transilluminator. The 1 kb DNA ladder marker was used to 50 μL DNase/RNase Free Water was added and then estimate the size of the DNA fragments. Sequencing for incubated for 1 min before centrifuged at 10,000 rpm for 1 identifying 16S rRNA bacteria was carried out by 1st Base min. The eluted DNA was then confirmed by DNA sequencing service (Axil Scientific Pte Ltd.) The electrophoresis with 1% agarose in 1x TAE Running sequencing results were analyzed using MegaX and Buffer at a voltage of 100 V.
Load more

Recommended publications
  • Dehydrated Culture Media Description Packaging Ref
    Product Catalogue 2016 © Liofilchem® s.r.l. Clinical and Industrial Microbiology Est. 1983 Dehydrated Culture Media Description Packaging Ref. A1 Medium APHA 500 g 610105 Basal liquid medium for fecal coliforms detection in water and food. 100 g 620105 TRITON X 100 supplement 5x5 mL 80046 Acetamide Agar 500 g 610312 Medium for differentiation of nonfermentative, Gram-negative bacteria, especially Pseudomonas aeruginosa, on the basis of acetamide utilization. Acetamide Broth 500 g 610313 Broth for the differentiation of nonfermentative, Gram-negative bacteria, especially Pseudomonas aeruginosa, on the basis of acetamide utilization. Aeromonas Agar Base 500 g 610048 Basal medium for selective isolation of Aeromonas spp. 100 g 620048 Ampicillin supplement 10 vials 81001 Alkaline Peptone Water APHA 500 g 610098 Liquid enrichment medium for Vibrio spp. isolation. 100 g 620098 Amies Transport Medium (with charcoal) 500 g 610152 Semi-solid medium for transport of clinical, environmental specimens and of 100 g 620152 microorganisms. 5 kg 6101525 Amies Transport Medium (w/o charcoal) 500 g 610191 Semi-solid medium for transport of clinical, environmental specimens and of 100 g 620191 microorganisms. 5 kg 6101915 Anaerobic Agar (Brewer) 500 g 610320 Medium for cultivating anaerobic microorganisms. Andrade Lactose Peptone Water 500 g 610118 Liquid medium for coliforms detection with andrade's indicator. 100 g 620118 Andrade Peptone Water 500 g 610119 Liquid enrichment medium with andrade's indicator. 100 g 620119 Antibiotic Agar No.1 E.P. 500 g 610314 Surface medium for the antibiotic assay by Agar-diffusion method. Antibiotic Broth No.3 U.S.P. 500 g 610316 Broth for turbidimetric assay of antibiotics.
    [Show full text]
  • Species Diversity of Lactic Acid Bacteria from Chilled Cooked Meat Products at Expiration Date in Belgian Retail
    SPECIES DIVERSITY OF LACTIC ACID BACTERIA FROM CHILLED COOKED MEAT PRODUCTS AT EXPIRATION DATE IN BELGIAN RETAIL Wim Geeraerts1, Vasileios Pothakos1, Luc De Vuyst1 and Frédéric Leroy1 1 Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium [email protected] Abstract – The bacterial communities of a wide (e.g., salt) and additives (e.g., sodium lactate). variety of chilled cooked meat products (29 different However, current practices often intend to reduce products), originating from pork and poultry, were the amount of salt and additives in view of subjected to extensive sampling. Samples were increasingly stringent consumer demands [1]. As a stored at 4 °C and analyzed at expiration date. result of the typical conditions prevailing in the Bacterial isolates were obtained from MRS agar, packaged and chilled cooked meat products, modified MRS agar, and M17 agar. Next, a specific microbiota develop. Usually, these procedure consisting of (GTG)5-PCR fingerprinting of genomic DNA followed by numerical clustering microbiota mostly consist of psychrophilic and was performed and for each cluster the identity of a psychrotolerant lactic acid bacteria (LAB), in selection of representative isolates was determined particular species of the genera Carnobacterium, by sequencing of the 16S rRNA gene. Based on the Enterococcus, Lactobacillus, and Leuconostoc [2- preliminary results, seven lactic acid bacterium 5]. Some of these LAB have only moderate effects (LAB) species were retrieved and belonged to the on the sensory status, whereas others have a clear following genera: Carnobacterium, Leuconostoc, ability to cause spoilage, including slime Lactobacillus, and Vagococcus.
    [Show full text]
  • Microbiological and Metagenomic Characterization of a Retail Delicatessen Galotyri-Like Fresh Acid-Curd Cheese Product
    fermentation Article Microbiological and Metagenomic Characterization of a Retail Delicatessen Galotyri-Like Fresh Acid-Curd Cheese Product John Samelis 1,* , Agapi I. Doulgeraki 2,* , Vasiliki Bikouli 2, Dimitrios Pappas 3 and Athanasia Kakouri 1 1 Dairy Research Department, Hellenic Agricultural Organization ‘DIMITRA’, Katsikas, 45221 Ioannina, Greece; [email protected] 2 Hellenic Agricultural Organization ‘DIMITRA’, Institute of Technology of Agricultural Products, 14123 Lycovrissi, Greece; [email protected] 3 Skarfi EPE—Pappas Bros Traditional Dairy, 48200 Filippiada, Greece; [email protected] * Correspondence: [email protected] (J.S.); [email protected] (A.I.D.); Tel.: +30-2651094789 (J.S.); +30-2102845940 (A.I.D.) Abstract: This study evaluated the microbial quality, safety, and ecology of a retail delicatessen Galotyri-like fresh acid-curd cheese traditionally produced by mixing fresh natural Greek yogurt with ‘Myzithrenio’, a naturally fermented and ripened whey cheese variety. Five retail cheese batches (mean pH 4.1) were analyzed for total and selective microbial counts, and 150 presumptive isolates of lactic acid bacteria (LAB) were characterized biochemically. Additionally, the most and the least diversified batches were subjected to a culture-independent 16S rRNA gene sequencing analysis. LAB prevailed in all cheeses followed by yeasts. Enterobacteria, pseudomonads, and staphylococci were present as <100 viable cells/g of cheese. The yogurt starters Streptococcus thermophilus and Lactobacillus delbrueckii were the most abundant LAB isolates, followed by nonstarter strains of Lactiplantibacillus, Lacticaseibacillus, Enterococcus faecium, E. faecalis, and Leuconostoc mesenteroides, Citation: Samelis, J.; Doulgeraki, A.I.; whose isolation frequency was batch-dependent. Lactococcus lactis isolates were sporadic, except Bikouli, V.; Pappas, D.; Kakouri, A. Microbiological and Metagenomic for one cheese batch.
    [Show full text]
  • Next Generation Microbiology for the Future
    Next Generation Microbiology for the Future www.msk.or.kr | 1 2014 INTERNATIONAL MEETING of the MICROBIOLOGICAL SOCIETY of KOREA 2 | 2014 International Meeting of the Microbiological Society of Korea 2014 INTERNATIONAL MEETING of Next Generationthe MICROBIOLOGICAL Microbiology for the Future SOCIETY of KOREA Contents • Timetable ············································································································································ 4 • Floor Plan ··········································································································································· 5 • Scientific Programs ···························································································································· 6 • Plenary Lectures······························································································································· 23 PL1 ······································································································································· 24 PL2 ······································································································································· 25 PL3 ······································································································································· 26 PL4 ······································································································································· 27 • Symposia ··········································································································································
    [Show full text]
  • BD Diagnostics - Diagnostic Systems
    Product Catalogue BD Diagnostics - Diagnostic Systems BD - your partner in excellence BD is a leading global medical of diagnosing infectious diseases approximately 28,000 people in technology company that develops, and cancers, and advancing more than 50 countries throughout manufacturers and sells medical research, discovery and production the world. The Company serves devices, instrument systems of new drugs and vaccines. BD’s healthcare institutions, life science and reagents. The Company is capabilities are instrumental in researchers, clinical laboratories, dedicated to improving people’s combating many of the world’s the pharmaceutical industry and health throughout the world. BD is most pressing diseases. Founded in the general public. focused on improving drug delivery, 1897 and headquartered in Franklin enhancing the quality and speed Lakes, New Jersey, BD employs BD Medical BD Diagnostics BD Biosciences > Diabetes Care > Diagnostic Systems > Discovery Labware > Medical Surgical Systems > Preanalytical Systems > Cell Analysis > Ophthalmic Systems > Pharmaceutical Systems BD Medical is among the world’s BD Diagnostics is a leading BD Biosciences is one of the leading suppliers of medical provider of products for the world’s leading businesses bringing devices. BD built the first ever safe collection and transport innovative tools to life scientists, manufacturing facility in the US of diagnostic specimens and clinical researchers and clinicians. to produce syringes and needles instruments for quick, accurate Our customers are involved in in 1906 and has been the leading analysis across a broad range of basic research, drug and vaccine innovator in injection and infusion- infectious diseases, including the discovery and development, based drug delivery ever since. growing problem of healthcare- biopharmaceutical production, associated infections (HAIs).
    [Show full text]
  • Journal of BIOLOGICAL RESEARCHES Volume 24| No
    ISSN: 08526834 | E-ISSN: 2337-389X Journal of BIOLOGICAL RESEARCHES Volume 24| No. 2| June| 2019 Original Article Profile of microbial community of naturally fermented Sumbawa mare’s milk using next-generation sequencing Yoga Dwi Jatmiko*, Irfan Mustafa, Tri Ardyati Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, Indonesia Abstract This study aimed to investigate the bacterial and fungal/yeast diversity in naturally fermented Sumbawa mare’s milk through a next-generation se- quencing approach, and evaluate the quality of fermented mare’s milk based on the presence of pathogenic or undesirable microorganisms. Microbial density determined using plate count agar (total aerobic bacteria), de Man Rogosa Sharpe agar (Lactobacillus), M17 agar (Lactococcus) and yeast peptone dextrose agar supplemented with streptomycin 50 ppm (yeast). Nutritional content and acidity level of each fermented milk sample were also evaluated. Genomic DNA was extracted using FastDNA Spin (MPBIO). The total gDNA was further analyzed using illumina high-throughput se- quencing (paired-end reads), and the sequence results were analysed using QIIME v.1.9.1 to generate diversity profiles. The difference in nutrient content of mare’s milk was thought to affect the density and diversity of microbes that were able to grow. Fermented mare’s milk samples from Sum- bawa had the highest bacterial diversity compared to samples from Bima and Dompu. However, fermented mare’s milk from Dompu had the best quality which was indicated by the absence of bacteria that have the potential to be pathogenic or food spoilage, such as members of the Enterobacte- riaceae family (Enterobacter, Klebsiella and Escherichia-Shigella) and Pseudomonas.
    [Show full text]
  • BD Industry Catalog
    PRODUCT CATALOG INDUSTRIAL MICROBIOLOGY BD Diagnostics Diagnostic Systems Table of Contents Table of Contents 1. Dehydrated Culture Media and Ingredients 5. Stains & Reagents 1.1 Dehydrated Culture Media and Ingredients .................................................................3 5.1 Gram Stains (Kits) ......................................................................................................75 1.1.1 Dehydrated Culture Media ......................................................................................... 3 5.2 Stains and Indicators ..................................................................................................75 5 1.1.2 Additives ...................................................................................................................31 5.3. Reagents and Enzymes ..............................................................................................75 1.2 Media and Ingredients ...............................................................................................34 1 6. Identification and Quality Control Products 1.2.1 Enrichments and Enzymes .........................................................................................34 6.1 BBL™ Crystal™ Identification Systems ..........................................................................79 1.2.2 Meat Peptones and Media ........................................................................................35 6.2 BBL™ Dryslide™ ..........................................................................................................80
    [Show full text]
  • Analysis of the Kefir and Koumiss Microbiota with the Focus on Certain Functional Properties of Selected Lactic Acid Bacteria
    B. Yusuf and U. Gürkan: Kefir and koumiss microbiota and functional properties of selected lactic acid bacteria Original scientific paper UDK: 637.146.1 Analysis of the kefir and koumiss microbiota with the focus on certain functional properties of selected lactic acid bacteria DOI: 10.15567/mljekarstvo.2021.0204 Biçer Yusuf*, Uçar Gürkan Selcuk University, Veterinary Faculty, Food Hygiene and Technology Department, 42130 Konya, Turkey Received: 02.05.2020. / Accepted: 16.02.2021. __________________ *Corresponding author: [email protected] Abstract The aim of this research was to determine the microbiota of commercial kefir, koumiss and homemade kefir samples using metagenomic analysis and compare some probiotic properties of lactic acid bacteria isolated from these beverages and Lactobacillus casei, used in yakult production. One koumiss, 5 commercially available kefir beverages with different brands, and 1 homemade kefir were used as samples. Microbial diversity of kefir and koumiss samples were determined by metagenomic analysis, targeting V1-V2 region of 16S rRNA gene. Streptococcus thermophilus and Lactococcus lactis were detected as dominant in direct DNA isolation from commercially available kefir beverages. Lc. lactis and Leuconostoc mesenteroides were dom- inant in MRS agars, and Lc. lactis were dominant in M17 agars. In kefir beverages produced by kefir grains,Lb. kefiranofaciens was determined as the dominant bacteria. Lb. kefiri and Entero- coccus durans were found dominant in MRS and M17 agars respectively. Lb. kefiranofaciens, Lb. kefiri, and Str. thermophilus were the dominant bacterias of koumiss beverages. Microorgan- isms isolated from kefir and koumiss beverages were found to exhibit basic probiotic properties, similar to the lactic acid bacteria isolated from yakult.
    [Show full text]
  • Ready to Use Culture Media in 3 L Or 5 L Bags
    ® Product Catalogue 2017 © Liofilchem® s.r.l. Liofilchem Roseto degli Abruzzi, Italy Est. 1983 Clinical and Industrial Microbiology Ready culture media in 55 mm contact plates Individually packed in transparent blister # Product with minimum order Description Packaging Ref. Baird Parker Agar + Neutralizing 20 plates 15331 Selective medium for Staphylococcus aureus isolation with inactivation of disinfectants. Cetrimide Agar E.P. 20 plates 15332 Selective medium for Pseudomonas aeruginosa isolation. Cetrimide Agar + Neutralizing E.P. 20 plates 15386 # Selective medium for Pseudomonas aeruginosa isolation with inactivation of disinfectants. Legionella BCYE Agar ISO 11731 20 plates 15334 Non-selective medium for Legionella spp. isolation. ISO 11731-2 Legionella GVPC Agar ISO 11731 20 plates 15376 Selective medium for Legionella spp. isolation. ISO 11731-2 MacConkey Agar E.P. 20 plates 15329 Selective medium for gram-negatives isolation. Mannitol Salt Agar EP, USP, JP 20 plates 15328 Selective medium for staphylococci isolation. m-Faecal Coliform Agar 20 plates 15368 Selective medium for fecal coliforms isolation and enumeration. Plate Count Agar ISO 4833 20 plates 15325 Medium for surfaces and air total bacterial count. Plate Count Agar + Neutralizing ISO 4833 20 plates 15336 Medium for surfaces total bacterial count with inactivation of disinfectants. Plate Count Agar + Neutralizing (Irradiated) ISO 4833 20 plates 15336S Medium for surfaces total bacterial count with inactivation of disinfectants. Plate Count Agar + TTC + Neutralizing ISO 4833 20 plates 15360 Medium for surfaces total bacterial count with inactivation of disinfectants. Rose Bengal Agar + CAF APHA 20 plates 15374 Selective medium for yeasts and moulds isolation and enumeration. Rose Bengal Agar + CAF + Neutralizing 20 plates 15385 # Selective medium for yeasts and moulds isolation and enumeration.
    [Show full text]
  • Kelleherpr Phd2017.Pdf
    UCC Library and UCC researchers have made this item openly available. Please let us know how this has helped you. Thanks! Title Comparative and functional genomic analysis of dairy lactococci Author(s) Kelleher, Philip Publication date 2017 Original citation Kelleher, P. 2017. Comparative and functional genomic analysis of dairy lactococci. PhD Thesis, University College Cork. Type of publication Doctoral thesis Rights © 2017, Philip Kelleher. http://creativecommons.org/licenses/by-nc-nd/3.0/ Item downloaded http://hdl.handle.net/10468/4522 from Downloaded on 2021-10-09T07:19:49Z Comparative and Functional Genomic Analysis of Dairy Lactococci A thesis presented to the National University of Ireland, Cork by Philip Kelleher BSc Pharmaceutical Biotechnology, MSc Bioinformatics & Systems Biology For the degree of PhD in Microbiology School of Microbiology, National University of Ireland, Cork January 2017 Supervisor: Prof. Douwe van Sinderen Head of School: Prof. Gerald Fitzgerald General Table of Contents Abbreviations ………………………………………….…………………. 4 Abstract …………………………………………………….…………….. 6 Chapter I: General introduction ……………………………………….. 12 Chapter II: Performance and flavour-based characterisation of lactococcal starter cultures ……………………………….……………... 93 Chapter III: Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation …………….. 130 Chapter IV: Comparative genomic analysis of the Lactococcus lactis plasmidome and assessment of its technological properties …….…….. 183 Chapter V: Base
    [Show full text]
  • CDC Anaerobe 5% Sheep Blood Agar with Phenylethyl Alcohol (PEA) CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin (KV)
    Difco & BBL Manual Manual of Microbiological Culture Media Second Edition Editors Mary Jo Zimbro, B.S., MT (ASCP) David A. Power, Ph.D. Sharon M. Miller, B.S., MT (ASCP) George E. Wilson, MBA, B.S., MT (ASCP) Julie A. Johnson, B.A. BD Diagnostics – Diagnostic Systems 7 Loveton Circle Sparks, MD 21152 Difco Manual Preface.ind 1 3/16/09 3:02:34 PM Table of Contents Contents Preface ...............................................................................................................................................................v About This Manual ...........................................................................................................................................vii History of BD Diagnostics .................................................................................................................................ix Section I: Monographs .......................................................................................................................................1 History of Microbiology and Culture Media ...................................................................................................3 Microorganism Growth Requirements .............................................................................................................4 Functional Types of Culture Media ..................................................................................................................5 Culture Media Ingredients – Agars ...................................................................................................................6
    [Show full text]
  • Moroccan Camel Milk Microbiota, a Source of New Lactic Acid Bacteria
    Moroccan camel milk microbiota, a source of new lactic acid bacteria Zaina Kadri Promoter: Prof. Dr. Peter Vandamme Co-promoter: Prof. Dr. Mohamed Amar Co-promoter: Prof. Dr. Omar El Farricha Year: 2016 EXAMINATION COMMITTEE Prof. Dr. Saad Ibnsouda Koraichi (Chairman) Faculty of Sciences and Technology Sidi Mohamed Ben Abdellah University, Fez, Morocco Prof. Dr. Mohamed Amar (Promotor) National Center for Scientific and Technical Research, CNRST Laboratory of Microbiology and Molecular Biology, LMBM Rabat, Morocco Prof. Dr. Peter Vandamme (Promotor) Faculty of Sciences Ghent University, Ghent, Belgium Prof. Dr. Omar El Farricha (Promotor) Faculty of Sciences and Technology Sidi Mohamed Ben Abdellah University, Fez, Morocco Prof. Dr. Ir. Jean Swings Faculty of Sciences Ghent University, Ghent, Belgium Prof. Dr. Khalid Sendide Al Akhawayn University, Ifrane, Morocco Prof. Dr. Kurt Houf Faculty of Veterinary Medicine Ghent University, Ghent, Belgium Prof. Dr. Guissi Sanae Faculty of Sciences and Technology Sidi Mohamed Ben Abdellah University, Fez, Morocco Prof. Dr. Ibijbijen Jamal Faculty of Sciences Moulay Ismail University, Meknes, Morocco i ACKNOWLEDGEMENTS I would like to express my sincere gratitude and appreciation to the person who made all this possible, Prof. Dr. Mohamed Amar. You always dedicated me your time, expertise, and good proposal. I would like to express my special thanks for giving me the opportunity to do this work at the Laboratory of Microbiology and Molecular Biol- ogy of the National Center for Scientific and Technical Research. This study was only possible thanks to you. My genuine gratitude and gratefulness are extended to Prof. Peter Vandamme. Your tremendous guidance, your expertise, thoughtful insights into microbiology, un- derstanding, kindness and helpfulness would be the unforgettable blessing of this stage.
    [Show full text]