Drug Chemistry Procedure Manual 1

SECTION A: Preliminary Tests

Marquis Reagent ...... A - 1

Duquenois-Levine Reagent ...... A - 2

Cobalt Thiocyanate Reagent ...... A - 3

Ferric Chloride Reagent ...... A - 4

Koppanyi Reagent...... A - 5

Potassium Permanganate Reagent ...... A - 6

p-Dimethylaminobenzaldehyde Reagent (PDMAB) ...... A - 7

Froehde’s Reagent...... A - 8

Mecke’s Reagent...... A - 9

Silver Nitrate Reagent...... A - 10

Zwikker Reagent...... A - 11

Barium Chloride Reagent ...... A - 12

Methanolic Potassium Hydroxide Reagent ...... A - 13

Secondary Amine Reagent #1...... A - 14

Secondary Amine Reagent #2...... A - 15

SECTION B: Polarized Light Microscopy

Polarized Light Microscopy...... B - 1

5% Mercuric Chloride With Optional 0.05 N Hydrochloric Acid Solution...... B - 2

Wagenaar Reagent...... B - 3

Drug Chemistry Procedure Manual 2

SECTION B: Polarized Light Microscopy (continued)

Gold Chloride in 20% Acetic Acid With Optional 0.05 N Hydrochloric Acid Solution ...... B - 4

Free Acid Using 2% Sodium Hydroxide and 5% Sulfuric Acid Solutions...... B - 5

0.05 N Hydrochloric Acid...... B - 6

Methanol ...... B - 7

Distilled Water ...... B - 8

Volatility Test Using Gold Chloride in Water With Concentrated Sodium Hydroxide Solution ...... B - 9

Volatility Test Using Gold Chloride in Dilute Phosphoric Acid With 10% Sodium Hydroxide...... B - 10

50% Acetic Acid and Gold Chloride in 50% Acetic Acid...... B - 11

Microscopic Examination of Hashish Using Chloral Hydrate ...... B - 12

Microscopic Examination of Hashish Using Concentrated Sodium Hydroxide...... B - 13

Microscopic Examination of Hashish Using Chloroform ...... B - 14

Microscopic Examination of Hashish Using Petroleum Ether...... B - 15

SECTION C: Thin-Layer Chromatography

9:1 Developing Solvent...... C - 1

2:1 Developing Solvent...... C - 2

4:1 Developing Solvent...... C - 3

10:1 Developing Solvent (Ethanol) ...... C - 4

Drug Chemistry Procedure Manual 3

SECTION C: Thin-Layer Chromatography (continued)

10:1 Developing Solvent (Methanol)...... C - 5

T1 Developing Solvent ...... C - 6

18:1 Developing Solvent...... C - 7

S1 Developing Solvent ...... C - 8

1:1 Developing Solvent...... C - 9

T2 Developing Solvent ...... C - 10

Iodoplatinate Visualizing Reagent...... C - 11

p-DMAB Visualizing Reagent ...... C - 12

Fast Blue B Visualizing Reagent...... C - 13

Sulfuric Acid Visualizing Reagent ...... C - 14

Iodine Visualizing Reagent...... C - 15

SECTION D: Extractions and Separations

Extraction of Organic Bases ...... D - 1

Extraction of Organic Acids ...... D - 2

Extraction of Volatile Organic Bases ...... D - 3

Extraction of Amphoteric Compounds (including Morphine, Hydrocodone and other Alkaloids)...... D - 4

Dry Solvent Extractions of Drugs Utilizing Hexane/Ammonia ...... D - 5

Dry Solvent Extractions of Drugs Utilizing Chloroform/Ammonia...... D - 6

Separation of Organic Acids and Bases by Solvent Wash...... D - 7

Drug Chemistry Procedure Manual 4

SECTION D: Extractions and Separations (continued)

Extraction of Psilocybe Mushrooms ...... D - 8

Extraction of Anabolic Steroids from Vegetable Oils ...... D - 9

Separation of Cocaine Base and Benzocaine Base...... D - 10

Separation of Cocaine Base and Procaine Base...... D - 11

Separation of Cocaine Hydrochloride and Dimethylerephthalate ...... D - 12

Separation of Cocaine Hydrochloride and Lidocaine Hydrochloride...... D - 13

Separation of Dextropropoxyphene Napsylate and Acetaminophen ...... D - 14

Extraction of Diethylpropion from a Time-release Preparation ...... D - 15

Extraction of Lysergic Acid Diethylamide (LSD) ...... D - 16

Extraction of Lysergic Acid Diethylamide (LSD) from Sugar Cubes ...... D - 17

Separation of Methamphetamine Hydrochloride and Dimethyl Sulfone ...... D - 18

Separation of Cocaine Hydrochloride and Nicotinamide ...... D - 19

Separation of Cocaine from Procaine, Benzocaine, Dimethylterephthalate, and Nicotinamide ...... D - 20

SECTION E: UV Spectrophotometry

Shimadzu Ultraviolet Spectrophotometer UV-260 / UV-265 ...... E - 1

SECTION F: Infrared Spectrophotometry

Mattson Galaxy 2020 FTIR Spectrophotometer...... F - 1

Nicolet Impact 410 FTIR Spectrophotometer...... F - 2

Drug Chemistry Procedure Manual 5

SECTION G: Gas Chromatograph/Mass Spectrometer

Hewlett-Packard 5890 Series II GC Interfaced to the Hewlett-Packard 5970 MSD ...... G - 1

SECTION H: Random Sampling

Random Sampling of Multiple Packages or Units ...... H - 1